NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10089R-3

Symbol

CG10089

Full Name

CG10089

CG No

CG10089

Old CG No

CG10089

Synonyms

CG10089

Accession No (Link to NCBI)

NM_168548.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Hosono C, Matsuda R, Adryan B, Samakovlis C.<br> Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.<br> Nat Cell Biol (2015) 17(12) 1569-76 [ PubMed ID = 26551273 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTATCGCGACTCCAAGGATCA

Primer Seq. 3'

aatctagaggtaccTGTCGCGGTTCTGCAGCAACT

Predicted Fragment Size

IR fragment full Seq

0001 CTATCGCGAC TCCAAGGATN CACGCTCAGC TGGAAAGATT CAAGATCTCG CACATCATCG 
0061 CCATACATGA CAGTCCGCGG CGTCTGCNTG CCGGACAAGC ACTACCTGTG CGTNGATGGC 
0121 CTCGGATACG CCAGACCAGA ATCTCTCCCA ATACTTTTTC CGTCTGCAAC GACNTTCATA 
0181 CGACGCCGCC CGGNTTCGCG AAGGCAACGT ACTCATCCAC TGCCTGGCGG GGATGTCGCN 
0241 CTCGGTNACC gtggccgTGG CCTATATCAT GACGGCCACA CACCTGAACT GGAAGGAGGC 
0301 ACTTAAGGTG GTTCGANCTG GTCGCGCTGT AGCCAATCCA AATGCTGGAT TCCAGAGCCA 
0361 ACTGCAGGAG TTTGAGCAGT TTAAGCTGTC CGAGGANCGC CGCCGCTTGA GGGAACGCTT 
0421 CCCATCCTCG GCTCTGGAGC AACTGGATCG CACTAAGGTG GCCACCGCGC TGGACAACTA 
0481 CCAGGAGTTG CTGCAGAANC GCGACA

in silico PCR Fragment

0001 CTATCGCGAC TCCAAGGAT- CACGCTCAGC TGGAAAGATT CAAGATCTCG CACATCATCG 
0061 CCATACATGA CAGTCCGCGG CGTTTGC-TG CCGGACAAGC ACTACCTGTG CGT-GATGGC 
0121 CTCGGATACG CCAGACCAGA ATCTCTCCCA ATAC-TTTTC CGTCTGCAAC GAC-TTCATA 
0181 C-ACGCCGCC CGGCTTCGCG AAGGCAACGT ACTCATCCAC TGCCTGGCGG GGATGTCGCG 
0241 CTCGGTGACC GTGGCCGTGG CCTATATCAT GACGGCCACA CACCTGAACT GGAAGGAGGC 
0301 ACTTAAGGTG GTTCGAGCTG GTCGCGCTGT AGCCAATCCA AATGCTGGAT TCCAGAGCCA 
0361 ACTGCAGGAG TTTGAGCAGT TTAAGCTGTC CGAGGAACGC CGCCGCTTGA GGGAACGCTT 
0421 CCCATCCTCG GCTCTGGAGC AACTGGATCG CACTAAGGTG GCCACCGCGC TGGACAACTA 
0481 CCAGGAGTTG CTGCAGAACC GCGACA

Assemble Data

<PRE>

10089R-3.IR_full       1   CTATCGCGACTCCAAGGAT<span class="snp"><tt>N</tt></span>CACGCTCAGCTGGAAAGATTCAAGATCTCGCACATCATCG 60
                           |||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||
10089R-3.in silico     1   CTATCGCGACTCCAAGGAT<span class="snp"><tt>-</tt></span>CACGCTCAGCTGGAAAGATTCAAGATCTCGCACATCATCG 60


10089R-3.IR_full       61  CCATACATGACAGTCCGCGGCGT<span class="snp"><tt>C</tt></span>TGC<span class="snp"><tt>N</tt></span>TGCCGGACAAGCACTACCTGTGCGT<span class="snp"><tt>N</tt></span>GATGGC 120
                           |||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||
10089R-3.in silico     61  CCATACATGACAGTCCGCGGCGT<span class="snp"><tt>T</tt></span>TGC<span class="snp"><tt>-</tt></span>TGCCGGACAAGCACTACCTGTGCGT<span class="snp"><tt>-</tt></span>GATGGC 120


10089R-3.IR_full       121 CTCGGATACGCCAGACCAGAATCTCTCCCAATAC<span class="snp"><tt>T</tt></span>TTTTCCGTCTGCAACGAC<span class="snp"><tt>N</tt></span>TTCATA 180
                           ||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||
10089R-3.in silico     121 CTCGGATACGCCAGACCAGAATCTCTCCCAATAC<span class="snp"><tt>-</tt></span>TTTTCCGTCTGCAACGAC<span class="snp"><tt>-</tt></span>TTCATA 180


10089R-3.IR_full       181 C<span class="snp"><tt>G</tt></span>ACGCCGCCCGG<span class="snp"><tt>N</tt></span>TTCGCGAAGGCAACGTACTCATCCACTGCCTGGCGGGGATGTCGC<span class="snp"><tt>N</tt></span> 240
                           |<span class="snp"><tt>&nbsp;</tt></span>|||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
10089R-3.in silico     181 C<span class="snp"><tt>-</tt></span>ACGCCGCCCGG<span class="snp"><tt>C</tt></span>TTCGCGAAGGCAACGTACTCATCCACTGCCTGGCGGGGATGTCGC<span class="snp"><tt>G</tt></span> 240


10089R-3.IR_full       241 CTCGGT<span class="snp"><tt>N</tt></span>ACCGTGGCCGTGGCCTATATCATGACGGCCACACACCTGAACTGGAAGGAGGC 300
                           ||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
10089R-3.in silico     241 CTCGGT<span class="snp"><tt>G</tt></span>ACCGTGGCCGTGGCCTATATCATGACGGCCACACACCTGAACTGGAAGGAGGC 300


10089R-3.IR_full       301 ACTTAAGGTGGTTCGA<span class="snp"><tt>N</tt></span>CTGGTCGCGCTGTAGCCAATCCAAATGCTGGATTCCAGAGCCA 360
                           ||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||
10089R-3.in silico     301 ACTTAAGGTGGTTCGA<span class="snp"><tt>G</tt></span>CTGGTCGCGCTGTAGCCAATCCAAATGCTGGATTCCAGAGCCA 360


10089R-3.IR_full       361 ACTGCAGGAGTTTGAGCAGTTTAAGCTGTCCGAGGA<span class="snp"><tt>N</tt></span>CGCCGCCGCTTGAGGGAACGCTT 420
                           ||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
10089R-3.in silico     361 ACTGCAGGAGTTTGAGCAGTTTAAGCTGTCCGAGGA<span class="snp"><tt>A</tt></span>CGCCGCCGCTTGAGGGAACGCTT 420


10089R-3.IR_full       421 CCCATCCTCGGCTCTGGAGCAACTGGATCGCACTAAGGTGGCCACCGCGCTGGACAACTA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10089R-3.in silico     421 CCCATCCTCGGCTCTGGAGCAACTGGATCGCACTAAGGTGGCCACCGCGCTGGACAACTA 480


10089R-3.IR_full       481 CCAGGAGTTGCTGCAGAA<span class="snp"><tt>N</tt></span>CGCGACA 506
                           ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||
10089R-3.in silico     481 CCAGGAGTTGCTGCAGAA<span class="snp"><tt>C</tt></span>CGCGACA 506

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_140397.1	CG10089-RD, transcript variant D (CG10089), mRNA
100	482	0	0	0	NM_168548.1	CG10089-RA, transcript variant A (CG10089), mRNA
100	482	0	0	0	NM_168549.1	CG10089-RB, transcript variant B (CG10089), mRNA
100	482	0	0	0	NM_168550.1	CG10089-RC, transcript variant C (CG10089), mRNA
0.2	1	2	3	6	NM_001043084.1	CG34123-RD, transcript variant D (CG34123), mRNA
0.2	1	2	3	6	NM_001043083.1	CG34123-RB, transcript variant B (CG34123), mRNA
0.2	1	2	3	6	NM_001043085.1	CG34123-RC, transcript variant C (CG34123), mRNA
0	0	4	2	10	NM_168785.1	CG14080-RA, transcript variant A (Mkp3), mRNA
0	0	4	2	10	NM_140830.1	CG14080-RB, transcript variant B (Mkp3), mRNA
0	0	3	2	3	NM_132292.2	CG7246-RA (CG7246), mRNA
0	0	2	2	2	NM_134494.2	CG12235-RA (Arp11), mRNA
0	0	1	3	10	NM_133143.1	CG8002-RA (rictor), mRNA
0	0	1	3	2	NM_137958.2	CG3906-RA (CG3906), mRNA
0	0	1	2	4	NM_142868.1	CG13830-RA (CG13830), mRNA
0	0	1	2	4	NM_165678.2	CG1884-RB, transcript variant B (Not1), mRNA
0	0	1	2	4	NM_136653.3	CG1884-RA, transcript variant A (Not1), mRNA
0	0	1	2	4	NM_166056.1	CG10110-RB, transcript variant B (cpsf), mRNA
0	0	1	2	4	NM_206111.1	CG10110-RA, transcript variant A (cpsf), mRNA
0	0	1	2	3	NM_131940.1	CG15912-RA (CG15912), mRNA
0	0	1	2	3	NM_079845.2	CG7951-RA (sima), mRNA
0	0	1	2	2	NM_137148.2	CG10202-RA (CG10202), mRNA
0	0	1	2	2	NM_136571.2	CG8262-RA (CG8262), mRNA
0	0	1	2	2	NM_137833.1	CG4610-RA (CG4610), mRNA
0	0	0	7	8	NM_133113.1	CG7378-RA (CG7378), mRNA
0	0	0	7	7	NM_078523.2	CG2252-RB, transcript variant B (fs(1)h), mRNA
0	0	0	7	6	NM_206647.1	CG2252-RC, transcript variant C (fs(1)h), mRNA
0	0	0	7	6	NM_167144.2	CG2252-RA, transcript variant A (fs(1)h), mRNA
0	0	0	7	6	NM_206646.1	CG2252-RD, transcript variant D (fs(1)h), mRNA
0	0	0	7	6	NM_206645.1	CG2252-RE, transcript variant E (fs(1)h), mRNA
0	0	0	5	4	NM_170359.1	CG5508-RB, transcript variant B (CG5508), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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