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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10083R-2 |
| Symbol |
CG10083 |
Full Name |
CG10083 |
| CG No |
CG10083 |
Old CG No |
CG10083 |
| Synonyms |
CG10083 |
| Accession No (Link to NCBI) |
NM_140400.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.<br> The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.<br> Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Kamimura K, Koyama T, Habuchi H, Ueda R, Masu M, Kimata K, Nakato H.<br> Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling.<br> J Cell Biol (2006) 174(6) 773-8
[
PubMed ID = 16966419
]
[
RRC reference
]
Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo K, Ueda R, Gehring WJ, Furukubo-Tokunaga K.<br> Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.<br> Proc Natl Acad Sci U S A (2006) 103(39) 14417-22
[
PubMed ID = 16971484
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTCACCTTACCATCAATGCCC |
| Primer Seq. 3' |
aatctagaggtaccACCCACACGAGAGCCAATCAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTCACCTTAC CATCAATGCC CGGAACGAGG ACGATATTGA CTTGGATCGG CTTCTCAAGA
0061 AGCTGAGCAC CGTGAGCTCA GCNTACAGCT TTAAGGAACC TCGAGGCGCC ATGGAGGAGC
0121 AGAAGGCNCC GGTGGGAACC AACTATACTC GGGTCATTCC CACCAAGGAG TTAAACGCCA
0181 GCGTTATGCA GGACTTCTGG AAAAAGGAGG AGGCCGAGGA GAAATTGCGA CAGGAGGCAG
0241 AGAAGGAATC CAAGCGGCTT GAGCTTCAAA AGTTGGAGCA GGAGCAACGT AGTCGCGAGG
0301 AGAAGGAGCA CAAGGAGCGG GAGAAGTTGG TCATAAGCAC CACCAAGCTC CAGCCGGCCC
0361 ACGTACCCAT CAAAACGTCA CCGCAACCTT TGAGCCCTGA GAAAACTGCA CCAGGATTTG
0421 CCAACAATCT GACCGATgNG GAGCGCATGC GTCAGGCGAG GAACCAGGAG GCCCGCGAAT
0481 TGATTGGCTC TCGTGTGGGT |
| in silico PCR Fragment |
0001 CTCACCTTAC CATCAATGCC CGGAACGAGG ACGATATTGA CTTGGATCGG CTTCTCAAGA
0061 AGCTGAGCAC CGTGAGCTCA GCCTACAGCT TTAAGGAACC TCGAGGCGCC ATGGAGGAGC
0121 AGAAGGCGCC GGTGGGAACC AACTATACTC GGGTCATTCC CACCAAGGAG TTAAACGCCA
0181 GCGTTATGCA GGACTTCTGG AAAAAGGAGG AGGCCGAGGA GAAATTGCGA CAGGAGGCAG
0241 AGAAGGAATC CAAGCGGCTT GAGCTTCAAA AGTTGGAGCA GGAGCAACGT AGTCGCGAGG
0301 AGAAGGAGCA CAAGGAGCGG GAGAAGTTGG TCATAAGCAC CACCAAGCTC CAGCCGGCCC
0361 ACGTACCCAT CAAAACGTCA CCGCAACCTT TGAGCCCTGA GAAAACTGCA CCAGGATTTG
0421 CCAACAATCT GACCGATGCG GAGCGCATGC GTCAGGCGAG GAACCAGGAG GCCCGCGAAT
0481 TGATTGGCTC TCGTGTGGGT |
| Assemble Data |
<PRE>
10083R-2.IR_full 1 CTCACCTTACCATCAATGCCCGGAACGAGGACGATATTGACTTGGATCGGCTTCTCAAGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10083R-2.in silico 1 CTCACCTTACCATCAATGCCCGGAACGAGGACGATATTGACTTGGATCGGCTTCTCAAGA 60
10083R-2.IR_full 61 AGCTGAGCACCGTGAGCTCAGC<span class="snp"><tt>N</tt></span>TACAGCTTTAAGGAACCTCGAGGCGCCATGGAGGAGC 120
||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||
10083R-2.in silico 61 AGCTGAGCACCGTGAGCTCAGC<span class="snp"><tt>C</tt></span>TACAGCTTTAAGGAACCTCGAGGCGCCATGGAGGAGC 120
10083R-2.IR_full 121 AGAAGGC<span class="snp"><tt>N</tt></span>CCGGTGGGAACCAACTATACTCGGGTCATTCCCACCAAGGAGTTAAACGCCA 180
|||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||
10083R-2.in silico 121 AGAAGGC<span class="snp"><tt>G</tt></span>CCGGTGGGAACCAACTATACTCGGGTCATTCCCACCAAGGAGTTAAACGCCA 180
10083R-2.IR_full 181 GCGTTATGCAGGACTTCTGGAAAAAGGAGGAGGCCGAGGAGAAATTGCGACAGGAGGCAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10083R-2.in silico 181 GCGTTATGCAGGACTTCTGGAAAAAGGAGGAGGCCGAGGAGAAATTGCGACAGGAGGCAG 240
10083R-2.IR_full 241 AGAAGGAATCCAAGCGGCTTGAGCTTCAAAAGTTGGAGCAGGAGCAACGTAGTCGCGAGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10083R-2.in silico 241 AGAAGGAATCCAAGCGGCTTGAGCTTCAAAAGTTGGAGCAGGAGCAACGTAGTCGCGAGG 300
10083R-2.IR_full 301 AGAAGGAGCACAAGGAGCGGGAGAAGTTGGTCATAAGCACCACCAAGCTCCAGCCGGCCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10083R-2.in silico 301 AGAAGGAGCACAAGGAGCGGGAGAAGTTGGTCATAAGCACCACCAAGCTCCAGCCGGCCC 360
10083R-2.IR_full 361 ACGTACCCATCAAAACGTCACCGCAACCTTTGAGCCCTGAGAAAACTGCACCAGGATTTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10083R-2.in silico 361 ACGTACCCATCAAAACGTCACCGCAACCTTTGAGCCCTGAGAAAACTGCACCAGGATTTG 420
10083R-2.IR_full 421 CCAACAATCTGACCGATG<span class="snp"><tt>N</tt></span>GGAGCGCATGCGTCAGGCGAGGAACCAGGAGGCCCGCGAAT 480
||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||
10083R-2.in silico 421 CCAACAATCTGACCGATG<span class="snp"><tt>C</tt></span>GGAGCGCATGCGTCAGGCGAGGAACCAGGAGGCCCGCGAAT 480
10083R-2.IR_full 481 TGATTGGCTCTCGTGTGGGT 500
||||||||||||||||||||
10083R-2.in silico 481 TGATTGGCTCTCGTGTGGGT 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_140400.1 |
CG10083-RA (CG10083), mRNA |
| 0 |
0 |
6 |
4 |
26 |
NM_144032.1 |
CG14580-RA (CG14580), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_131983.2 |
CG17764-RA (CG17764), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_141332.2 |
CG1218-RA (CG1218), mRNA |
| 0 |
0 |
2 |
5 |
18 |
NM_138220.1 |
CG13889-RA (CG13889), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165189.1 |
CG17927-RB, transcript variant B (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165192.1 |
CG17927-RM, transcript variant M (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165191.1 |
CG17927-RL, transcript variant L (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165190.1 |
CG17927-RK, transcript variant K (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_078863.4 |
CG17927-RH, transcript variant H (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165188.1 |
CG17927-RI, transcript variant I (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165187.1 |
CG17927-RA, transcript variant A (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165186.1 |
CG17927-RD, transcript variant D (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165185.1 |
CG17927-RF, transcript variant F (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165184.1 |
CG17927-RJ, transcript variant J (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165183.1 |
CG17927-RE, transcript variant E (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165182.1 |
CG17927-RG, transcript variant G (Mhc), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_165181.1 |
CG17927-RC, transcript variant C (Mhc), mRNA |
| 0 |
0 |
2 |
4 |
8 |
NM_142363.4 |
CG5407-RB, transcript variant B (Sur-8), mRNA |
| 0 |
0 |
2 |
4 |
8 |
NM_169758.1 |
CG5407-RA, transcript variant A (Sur-8), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_168670.1 |
CG32158-RB, transcript variant B (CG32158), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_141589.2 |
CG11963-RA (CG11963), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_168493.1 |
CG17146-RB, transcript variant B (Adk1), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_165627.1 |
CG8740-RC, transcript variant C (CG8740), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_165626.1 |
CG8740-RB, transcript variant B (CG8740), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_136567.2 |
CG8740-RA, transcript variant A (CG8740), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_169798.1 |
CG31196-RD, transcript variant D (14-3-3epsilon), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_169797.1 |
CG31196-RB, transcript variant B (14-3-3epsilon), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_169796.1 |
CG31196-RA, transcript variant A (14-3-3epsilon), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_169799.1 |
CG31196-RC, transcript variant C (14-3-3epsilon), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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