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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1007R-2 |
| Symbol |
emc |
Full Name |
extra macrochaetae |
| CG No |
CG1007 |
Old CG No |
CG1007 |
| Synonyms |
Emc, CG1007, l(3)05592, Dm0688, 0977/09, 0587/01, 0203/10, 0094/26, gov, ms(3)61CD, Ach, l(3)j4E11, l(3)04322, emc |
| Accession No (Link to NCBI) |
NM_079152.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Puig-Barbe A, Dettmann S, Nirello VD, Moor H, Azami S, Edgar BA, Varga-Weisz P, Korzelius J, de Navascués J.<br> A bHLH interaction code controls bipotential differentiation and self-renewal in the Drosophila gut.<br> Cell Rep (2025) 44(3) 115398
[
PubMed ID = 40089983
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACGGGGAGAACGCCGAGATGA |
| Primer Seq. 3' |
aatctagaggtaccCTGTCTGCTGTCTTTCTCCGC |
| Predicted Fragment Size |
497 |
| IR fragment full Seq |
0001 ACGGGGAGAA CGCCGAGATG AAGATGTATC TGTCCAAACT GNAAGGACCT CGTTCCGTTC
0061 ATGCCCAAGA ACAGGAAGCT CACCAAGCTG GAGATCATCC AGCACGTCAT CGACTACATC
0121 TGCGACCTGC AGACCGAGCT GGAGACGCAC CCCGAGATGG GCAACTTCGA TTGCGGCANG
0181 CGCTCTGACG GCGGTGAACG GACTCCACGA GGACGAGGAC AGCGACATGG AGGATGCGGA
0241 TGNCGAGGCA GAAGCGGNAA GTCGATCCAG ATATCCTCGC CCAGCGCCTG AATGCCGAGC
0301 AGCCGGCGAN AAGTCNTCTA GTCCCGCCGC CCGTCTCCCG CTTACCGATC GCCAAACGCC
0361 CAACACTCTT GTGGCGCCCG CCCATCCGCA GCAGCATCAG CAGCAGCAGC AACTGCAACT
0421 GCAGCAGCAA CAACTGCAAT CACAGCAGCA ACTGTCCAAC AGTTTAGcAA CGCCACAGAA
0481 TGCGGAGAAA GACAGCAGAC AG |
| in silico PCR Fragment |
0001 ACGGGGAGAA CGCCGAGATG AAGATGTATC TGTCCAAACT G-AAGGACCT CGTTCCGTTC
0061 ATGCCCAAGA ACAGGAAGCT CACCAAGCTG GAGATCATCC AGCACGTCAT CGACTACATC
0121 TGCGACCTGC AGACCGAGCT GGAGACGCAC CCCGAGATGG GCAACTTCGA -TGCGGCAGC
0181 CGCTCTGACG GCGGTGAACG GACTCCACGA GGACGAGGAC AGCGACATGG AGGATGCGGA
0241 TGCCGAGGCA GAAGCGG-AA GTCGATCCAG ATATCCTCGC CCAGCGCCTG AATGCCGAGC
0301 AGCCGGCGA- AAGTC-TCTA GTCCCGCCGC CCGTCTCCCG CTTACCGATC GCCAAACGCC
0361 CAACACTCTT GTGGCGCCCG CCCATCCGCA GCAGCATCAG CAGCAGCAGC AACTGCAACT
0421 GCAGCAGCAA CAACTGCAAT CACAGCAGCA ACTGTCCAAC AGTTTAGCAA CGCCACAGAA
0481 TGCGGAGAAA GACAGCAGAC AG |
| Assemble Data |
<PRE>
1007R-2.IR_full 1 ACGGGGAGAACGCCGAGATGAAGATGTATCTGTCCAAACTG<span class="snp"><tt>N</tt></span>AAGGACCTCGTTCCGTTC 60
|||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||
1007R-2.in silico 1 ACGGGGAGAACGCCGAGATGAAGATGTATCTGTCCAAACTG<span class="snp"><tt>-</tt></span>AAGGACCTCGTTCCGTTC 60
1007R-2.IR_full 61 ATGCCCAAGAACAGGAAGCTCACCAAGCTGGAGATCATCCAGCACGTCATCGACTACATC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1007R-2.in silico 61 ATGCCCAAGAACAGGAAGCTCACCAAGCTGGAGATCATCCAGCACGTCATCGACTACATC 120
1007R-2.IR_full 121 TGCGACCTGCAGACCGAGCTGGAGACGCACCCCGAGATGGGCAACTTCGA<span class="snp"><tt>T</tt></span>TGCGGCA<span class="snp"><tt>N</tt></span><span class="snp"><tt>G</tt></span> 180
||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>
1007R-2.in silico 121 TGCGACCTGCAGACCGAGCTGGAGACGCACCCCGAGATGGGCAACTTCGA<span class="snp"><tt>-</tt></span>TGCGGCA<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span> 180
1007R-2.IR_full 181 CGCTCTGACGGCGGTGAACGGACTCCACGAGGACGAGGACAGCGACATGGAGGATGCGGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1007R-2.in silico 181 CGCTCTGACGGCGGTGAACGGACTCCACGAGGACGAGGACAGCGACATGGAGGATGCGGA 240
1007R-2.IR_full 241 TG<span class="snp"><tt>N</tt></span>CGAGGCAGAAGCGG<span class="snp"><tt>N</tt></span>AAGTCGATCCAGATATCCTCGCCCAGCGCCTGAATGCCGAGC 300
||<span class="snp"><tt> </tt></span>||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||
1007R-2.in silico 241 TG<span class="snp"><tt>C</tt></span>CGAGGCAGAAGCGG<span class="snp"><tt>-</tt></span>AAGTCGATCCAGATATCCTCGCCCAGCGCCTGAATGCCGAGC 300
1007R-2.IR_full 301 AGCCGGCGA<span class="snp"><tt>N</tt></span>AAGTC<span class="snp"><tt>N</tt></span>TCTAGTCCCGCCGCCCGTCTCCCGCTTACCGATCGCCAAACGCC 360
|||||||||<span class="snp"><tt> </tt></span>|||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||
1007R-2.in silico 301 AGCCGGCGA<span class="snp"><tt>-</tt></span>AAGTC<span class="snp"><tt>-</tt></span>TCTAGTCCCGCCGCCCGTCTCCCGCTTACCGATCGCCAAACGCC 360
1007R-2.IR_full 361 CAACACTCTTGTGGCGCCCGCCCATCCGCAGCAGCATCAGCAGCAGCAGCAACTGCAACT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1007R-2.in silico 361 CAACACTCTTGTGGCGCCCGCCCATCCGCAGCAGCATCAGCAGCAGCAGCAACTGCAACT 420
1007R-2.IR_full 421 GCAGCAGCAACAACTGCAATCACAGCAGCAACTGTCCAACAGTTTAGCAACGCCACAGAA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1007R-2.in silico 421 GCAGCAGCAACAACTGCAATCACAGCAGCAACTGTCCAACAGTTTAGCAACGCCACAGAA 480
1007R-2.IR_full 481 TGCGGAGAAAGACAGCAGACAG 502
||||||||||||||||||||||
1007R-2.in silico 481 TGCGGAGAAAGACAGCAGACAG 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
479 |
2 |
16 |
56 |
NM_079152.3 |
CG1007-RA (emc), mRNA |
| 1.87 |
9 |
55 |
134 |
259 |
NM_132665.1 |
CG15753-RA (CG15753), mRNA |
| 1.87 |
9 |
39 |
209 |
529 |
NM_169696.1 |
CG3992-RA, transcript variant A (srp), mRNA |
| 1.87 |
9 |
39 |
209 |
529 |
NM_169694.1 |
CG3992-RB, transcript variant B (srp), mRNA |
| 1.67 |
8 |
45 |
135 |
329 |
NM_165217.1 |
CG6667-RA, transcript variant A (dl), mRNA |
| 1.67 |
8 |
45 |
135 |
329 |
NM_165218.1 |
CG6667-RB, transcript variant B (dl), mRNA |
| 1.67 |
8 |
30 |
135 |
415 |
NM_134552.3 |
CG1412-RA (RhoGAP19D), mRNA |
| 1.67 |
8 |
29 |
149 |
328 |
NM_141240.1 |
CG14656-RA (CG14656), mRNA |
| 1.67 |
8 |
26 |
139 |
343 |
NM_001032019.1 |
CG3992-RD, transcript variant D (srp), mRNA |
| 1.67 |
8 |
23 |
79 |
219 |
NM_137046.3 |
CG6191-RA (CG6191), mRNA |
| 1.67 |
8 |
16 |
85 |
225 |
NM_001043260.1 |
CG34157-RD, transcript variant D (Dys), mRNA |
| 1.67 |
8 |
12 |
68 |
164 |
NM_165017.1 |
CG12287-RB, transcript variant B (pdm2), mRNA |
| 1.67 |
8 |
12 |
61 |
134 |
NM_130728.1 |
CG15240-RA (CG15240), mRNA |
| 1.67 |
8 |
3 |
29 |
58 |
NM_137567.1 |
CG9811-RA (Rgk1), mRNA |
| 1.46 |
7 |
98 |
400 |
1029 |
NM_080376.1 |
CG8118-RA, transcript variant A (mam), mRNA |
| 1.46 |
7 |
98 |
400 |
1029 |
NM_166030.1 |
CG8118-RB, transcript variant B (mam), mRNA |
| 1.25 |
6 |
16 |
67 |
187 |
NM_079318.2 |
CG10488-RA, transcript variant A (eyg), mRNA |
| 1.25 |
6 |
16 |
67 |
187 |
NM_001014582.1 |
CG10488-RB, transcript variant B (eyg), mRNA |
| 1.25 |
6 |
10 |
57 |
107 |
NM_206109.1 |
CG8118-RC, transcript variant C (mam), mRNA |
| 1.25 |
6 |
4 |
62 |
166 |
NM_164960.1 |
CG32830-RA (CG32830), mRNA |
| 1.25 |
6 |
4 |
21 |
56 |
NM_132350.1 |
CG15321-RA (CG15321), mRNA |
| 1.25 |
6 |
3 |
10 |
35 |
NM_136202.4 |
CG31678-RA (CG31678), mRNA |
| 1.04 |
5 |
29 |
104 |
269 |
NM_001032408.1 |
CG33956-RD, transcript variant D (kay), mRNA |
| 1.04 |
5 |
25 |
151 |
353 |
NM_134527.1 |
CG15322-RA (CG15322), mRNA |
| 1.04 |
5 |
12 |
83 |
176 |
NM_136672.2 |
CG1688-RA (CG1688), mRNA |
| 1.04 |
5 |
11 |
97 |
293 |
NM_142210.1 |
CG6118-RA (CG6118), mRNA |
| 1.04 |
5 |
9 |
62 |
146 |
NM_206684.2 |
CG1725-RE, transcript variant E (dlg1), mRNA |
| 1.04 |
5 |
9 |
62 |
146 |
NM_206682.2 |
CG1725-RG, transcript variant G (dlg1), mRNA |
| 1.04 |
5 |
9 |
62 |
146 |
NM_078565.3 |
CG1725-RD, transcript variant D (dlg1), mRNA |
| 1.04 |
5 |
9 |
62 |
146 |
NM_167282.2 |
CG1725-RA, transcript variant A (dlg1), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04350
:
TGF-beta signaling pathway
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