|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10076R-2 |
| Symbol |
spir |
Full Name |
spire |
| CG No |
CG10076 |
Old CG No |
CG10076 |
| Synonyms |
p150Spir, p150-Spir, 38C.37, 38C.34, l(2)08327, CG10076, Spir, CG18621, spir |
| Accession No (Link to NCBI) |
NM_165323.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Fairchild MJ, Islam F, Tanentzapf G.<br> Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.<br> PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGAAGACGCCTTCCTGTCCAC |
| Primer Seq. 3' |
aatctagaggtaccACGCTCTGTTGATGACGCTGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGAAGACGCC TTCCTGTCCA CATCGCCGGA CAGCGCCAAC GgcGATGCCC AACACAAGCT
0061 GCCAGCGNAC CAGCTGgcCA TGTCCTCCAG TGCGCATCCC CAGCAGGCGG GCCAAgcccG
0121 CCCNCNCATN CCTGCAAGCC TTCCACCGCT GCTCCTCGCC AGAACAGTGC GTCACGNTGC
0181 ACGACATCCT GGACTCCTTT AAGGCGCCGC TCTCCGAGGA CCAGGCCTGG GCCCTGATCC
0241 ACCAGTTCGC CGGGCTCTAC CACCAGGTGG CCGTACAAGC GCACACCTGT GCCGCGGACT
0301 ACGAGGCCGC CCTGCCCACC GGATTCGAGC TGCACTTCCA TCGCGACGGC AGCGTCCACT
0361 TCTCCGGCCC GGATCAGCTG ACGCCTAAGG AGCAACTGCA GCAGGAGCAG ATCCCCCTCC
0421 CCCCGCAGCA CGACGTGATT GTGGATCAGC CGGACCACAG CGCCAGCAGC AGCGGCGACA
0481 GCAGCGTCAT CAACAGAGCG T |
| in silico PCR Fragment |
0001 GGAAGACGCC TTCCTGTCCA CATCGCCGGA CAGCGCCAAC GGCGATGCCC AACACAAGCT
0061 GCCAGCGGAC CAGCTGGCCA TGTCCTCCAG TGCGCATCCC CAGCAGGCGG GCCAAGCCCG
0121 CCCGCTCAT- CCTGCAAGCC TTCCACCGCT GCTCCTCGCC AGAACAGTGC GTCACGCTGC
0181 ACGACATCCT GGACTCCTTT AAGGCGCCGC TCTCCGAGGA CCAGGCCTGG GCCCTGATCC
0241 ACCAGTTCGC CGGGCTCTAC CACCAGGTGG CCGTACAAGC GCACACCTGT GCCGCGGACT
0301 ACGAGGCCGC CCTGCCCACC GGATTCGAGC TGCACTTCCA TCGCGACGGC AGCGTCCACT
0361 TCTCCGGCCC GGATCAGCTG ACGCCTAAGG AGCAACTGCA GCAGGAGCAG ATCCCCCTCC
0421 CCCCGCAGCA CGACGTGATT GTGGATCAGC CGGACCACAG CGCCAGCAGC AGCGGCGACA
0481 GCAGCGTCAT CAACAGAGCG T |
| Assemble Data |
<PRE>
10076R-2.IR_full 1 GGAAGACGCCTTCCTGTCCACATCGCCGGACAGCGCCAACGGCGATGCCCAACACAAGCT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-2.in silico 1 GGAAGACGCCTTCCTGTCCACATCGCCGGACAGCGCCAACGGCGATGCCCAACACAAGCT 60
10076R-2.IR_full 61 GCCAGCG<span class="snp"><tt>N</tt></span>ACCAGCTGGCCATGTCCTCCAGTGCGCATCCCCAGCAGGCGGGCCAAGCCCG 120
|||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-2.in silico 61 GCCAGCG<span class="snp"><tt>G</tt></span>ACCAGCTGGCCATGTCCTCCAGTGCGCATCCCCAGCAGGCGGGCCAAGCCCG 120
10076R-2.IR_full 121 CCC<span class="snp"><tt>N</tt></span>C<span class="snp"><tt>N</tt></span>CAT<span class="snp"><tt>N</tt></span>CCTGCAAGCCTTCCACCGCTGCTCCTCGCCAGAACAGTGCGTCACG<span class="snp"><tt>N</tt></span>TGC 180
|||<span class="snp"><tt> </tt></span>|<span class="snp"><tt> </tt></span>|||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||
10076R-2.in silico 121 CCC<span class="snp"><tt>G</tt></span>C<span class="snp"><tt>T</tt></span>CAT<span class="snp"><tt>-</tt></span>CCTGCAAGCCTTCCACCGCTGCTCCTCGCCAGAACAGTGCGTCACG<span class="snp"><tt>C</tt></span>TGC 180
10076R-2.IR_full 181 ACGACATCCTGGACTCCTTTAAGGCGCCGCTCTCCGAGGACCAGGCCTGGGCCCTGATCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-2.in silico 181 ACGACATCCTGGACTCCTTTAAGGCGCCGCTCTCCGAGGACCAGGCCTGGGCCCTGATCC 240
10076R-2.IR_full 241 ACCAGTTCGCCGGGCTCTACCACCAGGTGGCCGTACAAGCGCACACCTGTGCCGCGGACT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-2.in silico 241 ACCAGTTCGCCGGGCTCTACCACCAGGTGGCCGTACAAGCGCACACCTGTGCCGCGGACT 300
10076R-2.IR_full 301 ACGAGGCCGCCCTGCCCACCGGATTCGAGCTGCACTTCCATCGCGACGGCAGCGTCCACT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-2.in silico 301 ACGAGGCCGCCCTGCCCACCGGATTCGAGCTGCACTTCCATCGCGACGGCAGCGTCCACT 360
10076R-2.IR_full 361 TCTCCGGCCCGGATCAGCTGACGCCTAAGGAGCAACTGCAGCAGGAGCAGATCCCCCTCC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-2.in silico 361 TCTCCGGCCCGGATCAGCTGACGCCTAAGGAGCAACTGCAGCAGGAGCAGATCCCCCTCC 420
10076R-2.IR_full 421 CCCCGCAGCACGACGTGATTGTGGATCAGCCGGACCACAGCGCCAGCAGCAGCGGCGACA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-2.in silico 421 CCCCGCAGCACGACGTGATTGTGGATCAGCCGGACCACAGCGCCAGCAGCAGCGGCGACA 480
10076R-2.IR_full 481 GCAGCGTCATCAACAGAGCGT 501
|||||||||||||||||||||
10076R-2.in silico 481 GCAGCGTCATCAACAGAGCGT 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
1 |
3 |
NM_165323.1 |
CG10076-RA, transcript variant A (spir), mRNA |
| 100 |
482 |
0 |
1 |
3 |
NM_080115.2 |
CG10076-RB, transcript variant B (spir), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_165324.1 |
CG10076-RD, transcript variant D (spir), mRNA |
| 0.82 |
4 |
2 |
2 |
4 |
NM_135727.1 |
CG5418-RA (CG5418), mRNA |
| 0.41 |
2 |
3 |
2 |
3 |
NM_134577.1 |
CG1529-RA (CG1529), mRNA |
| 0.2 |
1 |
2 |
18 |
54 |
NM_167330.2 |
CG17762-RD, transcript variant D (tomosyn), mRNA |
| 0.2 |
1 |
2 |
12 |
27 |
NM_167329.2 |
CG17762-RC, transcript variant C (tomosyn), mRNA |
| 0.2 |
1 |
2 |
12 |
27 |
NM_167328.2 |
CG17762-RA, transcript variant A (tomosyn), mRNA |
| 0.2 |
1 |
2 |
12 |
18 |
NM_132591.3 |
CG17762-RB, transcript variant B (tomosyn), mRNA |
| 0 |
0 |
6 |
15 |
107 |
NM_132246.2 |
CG10555-RA (CG10555), mRNA |
| 0 |
0 |
5 |
29 |
101 |
NM_169696.1 |
CG3992-RA, transcript variant A (srp), mRNA |
| 0 |
0 |
5 |
29 |
101 |
NM_169694.1 |
CG3992-RB, transcript variant B (srp), mRNA |
| 0 |
0 |
5 |
6 |
5 |
NM_164710.1 |
CG17378-RB, transcript variant B (CG17378), mRNA |
| 0 |
0 |
5 |
6 |
5 |
NM_135229.2 |
CG17378-RA, transcript variant A (CG17378), mRNA |
| 0 |
0 |
5 |
3 |
4 |
NM_135936.2 |
CG4993-RB, transcript variant B (PRL-1), mRNA |
| 0 |
0 |
5 |
3 |
4 |
NM_165150.1 |
CG4993-RA, transcript variant A (PRL-1), mRNA |
| 0 |
0 |
4 |
28 |
91 |
NM_078514.2 |
CG9653-RA (brk), mRNA |
| 0 |
0 |
4 |
17 |
50 |
NM_206356.2 |
CG33261-RB, transcript variant B (Trl), mRNA |
| 0 |
0 |
4 |
16 |
79 |
NM_168113.2 |
CG32423-RD, transcript variant D (alan-shepard), mRNA |
| 0 |
0 |
4 |
14 |
90 |
NM_001038965.1 |
CG9924-RE, transcript variant E (CG9924), mRNA |
| 0 |
0 |
4 |
14 |
41 |
NM_001038926.1 |
CG33261-RI, transcript variant I (Trl), mRNA |
| 0 |
0 |
4 |
11 |
25 |
NM_167388.1 |
CG32606-RA (CG32606), mRNA |
| 0 |
0 |
4 |
9 |
76 |
NM_001038742.1 |
CG6824-RD, transcript variant D (ovo), mRNA |
| 0 |
0 |
4 |
9 |
76 |
NM_080338.3 |
CG6824-RA, transcript variant A (ovo), mRNA |
| 0 |
0 |
4 |
8 |
101 |
NM_132172.2 |
CG15478-RA (CG15478), mRNA |
| 0 |
0 |
4 |
8 |
65 |
NM_132413.1 |
CG15295-RA (CG15295), mRNA |
| 0 |
0 |
4 |
6 |
51 |
NM_141636.1 |
CG16777-RA (CG16777), mRNA |
| 0 |
0 |
4 |
6 |
13 |
NM_136527.1 |
CG14755-RA (CG14755), mRNA |
| 0 |
0 |
3 |
25 |
55 |
NM_001014527.1 |
CG8787-RB, transcript variant B (Asx), mRNA |
| 0 |
0 |
3 |
25 |
55 |
NM_166057.1 |
CG8787-RA, transcript variant A (Asx), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04320
:
Dorso-ventral axis formation
|
|
|
