NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10076R-1

Symbol

spir

Full Name

spire

CG No

CG10076

Old CG No

CG10076

Synonyms

p150Spir, p150-Spir, 38C.37, 38C.34, l(2)08327, CG10076, Spir, CG18621, spir

Accession No (Link to NCBI)

NM_165323.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.<br> A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.<br> Genetics (2010) 186(3) 943-57 [ PubMed ID = 20813879 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGAAGACGCCTTCCTGTCCAC

Primer Seq. 3'

aatctagaggtaccACGCTCTGTTGATGACGCTGC

Predicted Fragment Size

IR fragment full Seq

0001 GGAAGACGCC TTCCTGTCCA CATCGCCGGA CAGCGCCAAC GgcGATGCCC AACACAAGCT 
0061 GCCAGCGNAC CAGCTGgcCA TGTCCTCCAG TGCGCATCCC CAGCAGGCGG GCCAAgcccG 
0121 CCCNCNCATN CCTGCAAGCC TTCCACCGCT GCTCCTCGCC AGAACAGTGC GTCACGNTGC 
0181 ACGACATCCT GGACTCCTTT AAGGCGCCGC TCTCCGAGGA CCAGGCCTGG GCCCTGATCC 
0241 ACCAGTTCGC CGGGCTCTAC CACCAGGTGG CCGTACAAGC GCACACCTGT GCCGCGGACT 
0301 ACGAGGCCGC CCTGCCCACC GGATTCGAGC TGCACTTCCA TCGCGACGGC AGCGTCCACT 
0361 TCTCCGGCCC GGATCAGCTG ACGCCTAAGG AGCAACTGCA GCAGGAGCAG ATCCCCCTCC 
0421 CCCCGCAGCA CGACGTGATT GTGGATCAGC CGGACCACAG CGCCAGCAGC AGCGGCGACA 
0481 GCAGCGTCAT CAACAGAGCG T

in silico PCR Fragment

0001 GGAAGACGCC TTCCTGTCCA CATCGCCGGA CAGCGCCAAC GGCGATGCCC AACACAAGCT 
0061 GCCAGCGGAC CAGCTGGCCA TGTCCTCCAG TGCGCATCCC CAGCAGGCGG GCCAAGCCCG 
0121 CCCGCTCAT- CCTGCAAGCC TTCCACCGCT GCTCCTCGCC AGAACAGTGC GTCACGCTGC 
0181 ACGACATCCT GGACTCCTTT AAGGCGCCGC TCTCCGAGGA CCAGGCCTGG GCCCTGATCC 
0241 ACCAGTTCGC CGGGCTCTAC CACCAGGTGG CCGTACAAGC GCACACCTGT GCCGCGGACT 
0301 ACGAGGCCGC CCTGCCCACC GGATTCGAGC TGCACTTCCA TCGCGACGGC AGCGTCCACT 
0361 TCTCCGGCCC GGATCAGCTG ACGCCTAAGG AGCAACTGCA GCAGGAGCAG ATCCCCCTCC 
0421 CCCCGCAGCA CGACGTGATT GTGGATCAGC CGGACCACAG CGCCAGCAGC AGCGGCGACA 
0481 GCAGCGTCAT CAACAGAGCG T

Assemble Data

<PRE>

10076R-1.IR_full       1   GGAAGACGCCTTCCTGTCCACATCGCCGGACAGCGCCAACGGCGATGCCCAACACAAGCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-1.in silico     1   GGAAGACGCCTTCCTGTCCACATCGCCGGACAGCGCCAACGGCGATGCCCAACACAAGCT 60


10076R-1.IR_full       61  GCCAGCG<span class="snp"><tt>N</tt></span>ACCAGCTGGCCATGTCCTCCAGTGCGCATCCCCAGCAGGCGGGCCAAGCCCG 120
                           |||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-1.in silico     61  GCCAGCG<span class="snp"><tt>G</tt></span>ACCAGCTGGCCATGTCCTCCAGTGCGCATCCCCAGCAGGCGGGCCAAGCCCG 120


10076R-1.IR_full       121 CCC<span class="snp"><tt>N</tt></span>C<span class="snp"><tt>N</tt></span>CAT<span class="snp"><tt>N</tt></span>CCTGCAAGCCTTCCACCGCTGCTCCTCGCCAGAACAGTGCGTCACG<span class="snp"><tt>N</tt></span>TGC 180
                           |||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||
10076R-1.in silico     121 CCC<span class="snp"><tt>G</tt></span>C<span class="snp"><tt>T</tt></span>CAT<span class="snp"><tt>-</tt></span>CCTGCAAGCCTTCCACCGCTGCTCCTCGCCAGAACAGTGCGTCACG<span class="snp"><tt>C</tt></span>TGC 180


10076R-1.IR_full       181 ACGACATCCTGGACTCCTTTAAGGCGCCGCTCTCCGAGGACCAGGCCTGGGCCCTGATCC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-1.in silico     181 ACGACATCCTGGACTCCTTTAAGGCGCCGCTCTCCGAGGACCAGGCCTGGGCCCTGATCC 240


10076R-1.IR_full       241 ACCAGTTCGCCGGGCTCTACCACCAGGTGGCCGTACAAGCGCACACCTGTGCCGCGGACT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-1.in silico     241 ACCAGTTCGCCGGGCTCTACCACCAGGTGGCCGTACAAGCGCACACCTGTGCCGCGGACT 300


10076R-1.IR_full       301 ACGAGGCCGCCCTGCCCACCGGATTCGAGCTGCACTTCCATCGCGACGGCAGCGTCCACT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-1.in silico     301 ACGAGGCCGCCCTGCCCACCGGATTCGAGCTGCACTTCCATCGCGACGGCAGCGTCCACT 360


10076R-1.IR_full       361 TCTCCGGCCCGGATCAGCTGACGCCTAAGGAGCAACTGCAGCAGGAGCAGATCCCCCTCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-1.in silico     361 TCTCCGGCCCGGATCAGCTGACGCCTAAGGAGCAACTGCAGCAGGAGCAGATCCCCCTCC 420


10076R-1.IR_full       421 CCCCGCAGCACGACGTGATTGTGGATCAGCCGGACCACAGCGCCAGCAGCAGCGGCGACA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10076R-1.in silico     421 CCCCGCAGCACGACGTGATTGTGGATCAGCCGGACCACAGCGCCAGCAGCAGCGGCGACA 480


10076R-1.IR_full       481 GCAGCGTCATCAACAGAGCGT 501
                           |||||||||||||||||||||
10076R-1.in silico     481 GCAGCGTCATCAACAGAGCGT 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	1	3	NM_165323.1	CG10076-RA, transcript variant A (spir), mRNA
100	482	0	1	3	NM_080115.2	CG10076-RB, transcript variant B (spir), mRNA
100	482	0	0	0	NM_165324.1	CG10076-RD, transcript variant D (spir), mRNA
0.82	4	2	2	4	NM_135727.1	CG5418-RA (CG5418), mRNA
0.41	2	3	2	3	NM_134577.1	CG1529-RA (CG1529), mRNA
0.2	1	2	18	54	NM_167330.2	CG17762-RD, transcript variant D (tomosyn), mRNA
0.2	1	2	12	27	NM_167329.2	CG17762-RC, transcript variant C (tomosyn), mRNA
0.2	1	2	12	27	NM_167328.2	CG17762-RA, transcript variant A (tomosyn), mRNA
0.2	1	2	12	18	NM_132591.3	CG17762-RB, transcript variant B (tomosyn), mRNA
0	0	6	15	107	NM_132246.2	CG10555-RA (CG10555), mRNA
0	0	5	29	101	NM_169696.1	CG3992-RA, transcript variant A (srp), mRNA
0	0	5	29	101	NM_169694.1	CG3992-RB, transcript variant B (srp), mRNA
0	0	5	6	5	NM_164710.1	CG17378-RB, transcript variant B (CG17378), mRNA
0	0	5	6	5	NM_135229.2	CG17378-RA, transcript variant A (CG17378), mRNA
0	0	5	3	4	NM_135936.2	CG4993-RB, transcript variant B (PRL-1), mRNA
0	0	5	3	4	NM_165150.1	CG4993-RA, transcript variant A (PRL-1), mRNA
0	0	4	28	91	NM_078514.2	CG9653-RA (brk), mRNA
0	0	4	17	50	NM_206356.2	CG33261-RB, transcript variant B (Trl), mRNA
0	0	4	16	79	NM_168113.2	CG32423-RD, transcript variant D (alan-shepard), mRNA
0	0	4	14	90	NM_001038965.1	CG9924-RE, transcript variant E (CG9924), mRNA
0	0	4	14	41	NM_001038926.1	CG33261-RI, transcript variant I (Trl), mRNA
0	0	4	11	25	NM_167388.1	CG32606-RA (CG32606), mRNA
0	0	4	9	76	NM_001038742.1	CG6824-RD, transcript variant D (ovo), mRNA
0	0	4	9	76	NM_080338.3	CG6824-RA, transcript variant A (ovo), mRNA
0	0	4	8	101	NM_132172.2	CG15478-RA (CG15478), mRNA
0	0	4	8	65	NM_132413.1	CG15295-RA (CG15295), mRNA
0	0	4	6	51	NM_141636.1	CG16777-RA (CG16777), mRNA
0	0	4	6	13	NM_136527.1	CG14755-RA (CG14755), mRNA
0	0	3	25	55	NM_001014527.1	CG8787-RB, transcript variant B (Asx), mRNA
0	0	3	25	55	NM_166057.1	CG8787-RA, transcript variant A (Asx), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme04320 : Dorso-ventral axis formation

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