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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10066R-4

Symbol

Fer1

Full Name

48 related 1

CG No

CG33323

Old CG No

CG10066

Synonyms

CG10066, CG33323, BcDNA:RH30329, CG14611, Fer1

Accession No (Link to NCBI)

NM_206455.1

Inserted Chr.

Insertional Mutation

semi-lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

pupal semi-lethal

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Jafari S, Alkhori L, Schleiffer A, Brochtrup A, Hummel T, Alenius M.<br> Combinatorial activation and repression by seven transcription factors specify Drosophila odorant receptor expression.<br> PLoS Biol (2012) 10(3) e1001280 [ PubMed ID = 22427741 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAATTTCGACTTGGAGGCCAC

Primer Seq. 3'

aatctagaggtaccGGATAATTTTCTTGGGCGGCT

Predicted Fragment Size

IR fragment full Seq

0001 CAATTTCGAC TTGGAGGCCA CGATGGCGCG CCACTTCTTC GAGGGATCGC AGGCCACCAA 
0061 CGCCTCCACC TCCAGCTCCG ACTATTTTTT TTGGGGACGA GCACAGCTCG GAGAGCGATG 
0121 ACGAGGACGA TGCCTATAGC AGTGGCTTCA ACAGCGACCA GGAGGAACAC CGAGAAGACC 
0181 CGGCGGAGCC ACAAGCCCCG GCGCTTGAAG TGCGCCTCCC AAATGGCCCA GCAGCGGCAG 
0241 GCGGCCAATC TCCGCGAGCG CCGCCGGATG CAGAGCATCA ACGAGGCGTT CGAGGGTCTG 
0301 CGCACCCACA TTCCCACGCT GCCCTACGAG AAGCGACTAA GCAAGGTGGA CACACTCAAG 
0361 CTGGCCATAA GCTACATAAC CTTCCTCAGC GAGATGGTCA AGAAGGACAA GAACGGCAAC 
0421 GAGCCGGGCC TAAGTCTGCA GCGTAACTAC CAAAAGGAGC CGCCCAAGAA AATTATCC

in silico PCR Fragment

0001 CAATTTCGAC TTGGAGGCCA CGATGGCGCG CCACTTCTTC GAGGGATCGC AGGCCACCAA 
0061 CGCCTCCACC TCCAGCTCCG ACTA-TTTTT TTGGGGACGA GCACAGCTCG GAGAGCGATG 
0121 ACGAGGACGA TGCCTATAGC AGTGGCTTCA ACAGCGACCA GGA-GAACAC CGAGAAGACC 
0181 CGGCGGAGCC ACAAGCCCCG GCGCTTGAAG TGCGCCTCCC AAATGGCCCA GCAGCGGCAG 
0241 GCGGCCAATC TCCGCGAGCG CCGCCGGATG CAGAGCATCA ACGAGGCGTT CGAGGGTCTG 
0301 CGCACCCACA TTCCCACGCT GCCCTACGAG AAGCGACTAA GCAAGGTGGA CACACTCAAG 
0361 CTGGCCATAA GCTACATAAC CTTCCTCAGC GAGATGGTCA AGAAGGACAA GAACGGCAAC 
0421 GAGCCGGGCC TAAGTCTGCA GCGTAACTAC CAAAAGGAGC CGCCCAAGAA AATTATCC

Assemble Data

<PRE>

10066R-4.IR_full       1   CAATTTCGACTTGGAGGCCACGATGGCGCGCCACTTCTTCGAGGGATCGCAGGCCACCAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-4.in silico     1   CAATTTCGACTTGGAGGCCACGATGGCGCGCCACTTCTTCGAGGGATCGCAGGCCACCAA 60


10066R-4.IR_full       61  CGCCTCCACCTCCAGCTCCGACTA<span class="snp"><tt>T</tt></span>TTTTTTTGGGGACGAGCACAGCTCGGAGAGCGATG 120
                           ||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||
10066R-4.in silico     61  CGCCTCCACCTCCAGCTCCGACTA<span class="snp"><tt>-</tt></span>TTTTTTTGGGGACGAGCACAGCTCGGAGAGCGATG 120


10066R-4.IR_full       121 ACGAGGACGATGCCTATAGCAGTGGCTTCAACAGCGACCAGGA<span class="snp"><tt>G</tt></span>GAACACCGAGAAGACC 180
                           |||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||
10066R-4.in silico     121 ACGAGGACGATGCCTATAGCAGTGGCTTCAACAGCGACCAGGA<span class="snp"><tt>-</tt></span>GAACACCGAGAAGACC 180


10066R-4.IR_full       181 CGGCGGAGCCACAAGCCCCGGCGCTTGAAGTGCGCCTCCCAAATGGCCCAGCAGCGGCAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-4.in silico     181 CGGCGGAGCCACAAGCCCCGGCGCTTGAAGTGCGCCTCCCAAATGGCCCAGCAGCGGCAG 240


10066R-4.IR_full       241 GCGGCCAATCTCCGCGAGCGCCGCCGGATGCAGAGCATCAACGAGGCGTTCGAGGGTCTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-4.in silico     241 GCGGCCAATCTCCGCGAGCGCCGCCGGATGCAGAGCATCAACGAGGCGTTCGAGGGTCTG 300


10066R-4.IR_full       301 CGCACCCACATTCCCACGCTGCCCTACGAGAAGCGACTAAGCAAGGTGGACACACTCAAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-4.in silico     301 CGCACCCACATTCCCACGCTGCCCTACGAGAAGCGACTAAGCAAGGTGGACACACTCAAG 360


10066R-4.IR_full       361 CTGGCCATAAGCTACATAACCTTCCTCAGCGAGATGGTCAAGAAGGACAAGAACGGCAAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-4.in silico     361 CTGGCCATAAGCTACATAACCTTCCTCAGCGAGATGGTCAAGAAGGACAAGAACGGCAAC 420


10066R-4.IR_full       421 GAGCCGGGCCTAAGTCTGCAGCGTAACTACCAAAAGGAGCCGCCCAAGAAAATTATCC 478
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-4.in silico     421 GAGCCGGGCCTAAGTCTGCAGCGTAACTACCAAAAGGAGCCGCCCAAGAAAATTATCC 478

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	458	0	0	0	NM_206455.1	CG33323-RA (Fer1), mRNA
0.87	4	2	3	2	NM_132240.2	CG10965-RA (Corp), mRNA
0.43	2	2	3	2	NM_140059.2	CG3448-RB (CG3448), mRNA
0.21	1	5	3	5	NM_166623.1	CG4051-RA (egl), mRNA
0	0	4	5	2	NM_133112.1	CG7349-RA (CG7349), mRNA
0	0	4	4	5	NM_135106.1	CG7236-RA (CG7236), mRNA
0	0	4	2	5	NM_057502.2	CG1322-RB, transcript variant B (zfh1), mRNA
0	0	4	2	5	NM_170522.2	CG1322-RC, transcript variant C (zfh1), mRNA
0	0	4	2	3	NM_170485.1	CG2139-RC, transcript variant C (aralar1), mRNA
0	0	4	2	3	NM_170487.1	CG2139-RB, transcript variant B (aralar1), mRNA
0	0	4	2	3	NM_143538.2	CG2139-RA, transcript variant A (aralar1), mRNA
0	0	4	2	3	NM_170486.1	CG2139-RD, transcript variant D (aralar1), mRNA
0	0	3	3	9	NM_142457.2	CG7669-RA (CG7669), mRNA
0	0	3	2	6	NM_135861.1	CG15287-RA (CG15287), mRNA
0	0	2	4	4	NM_164522.1	CG17257-RA, transcript variant A (CG17257), mRNA
0	0	2	4	4	NM_134897.2	CG17257-RB, transcript variant B (CG17257), mRNA
0	0	2	3	7	NM_166527.1	CG5820-RA, transcript variant A (Gp150), mRNA
0	0	2	3	7	NM_166528.1	CG5820-RB, transcript variant B (Gp150), mRNA
0	0	2	3	7	NM_166529.1	CG5820-RC, transcript variant C (Gp150), mRNA
0	0	2	3	7	NM_057701.3	CG5820-RD, transcript variant D (Gp150), mRNA
0	0	2	3	4	NM_165285.1	CG31797-RA (CG31797), mRNA
0	0	2	3	4	NM_078498.2	CG4336-RA (rux), mRNA
0	0	2	3	2	NM_144393.1	CG18747-RA (CG18747), mRNA
0	0	2	2	8	NM_078604.3	CG32592-RA (hiw), mRNA
0	0	2	2	4	NM_168038.1	CG32264-RD, transcript variant D (CG32264), mRNA
0	0	2	2	3	NM_134577.1	CG1529-RA (CG1529), mRNA
0	0	2	2	3	NM_142053.1	CG14366-RA (CG14366), mRNA
0	0	2	2	2	NM_132396.1	CG2222-RA (Psf3), mRNA
0	0	1	7	9	NM_080049.2	CG11614-RA (nkd), mRNA
0	0	1	6	6	NM_057757.3	CG2714-RA, transcript variant A (crm), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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