NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10062R-2

Symbol CG10062 Full Name CG10062

CG No CG10062 Old CG No CG10062

Synonyms anon-WO0140519.226, CG10062

Accession No (Link to NCBI) NM_137572.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564 [ PubMed ID = 34239451 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAGCATGGCCAACGAGGAGAG

Primer Seq. 3'

aatctagaggtaccACGGATGATTCGGTCCCGACT

Predicted Fragment Size

IR fragment full Seq

0001 CAGCATGGCC AACGAGGGAG AGCGCCGTGG AGTTCCTGCG CGCCGAGGTA GCCAAAGTGG 
0061 AGTCCGAAAT GAGCGACCTT CTGGAGATCG AAGTGGATGT GCAGCAGGCC AGTGGAGCCT 
0121 ACATGCACTG GGAGATGGTC AACATGTATC AGGGCATTCA GAATGTGGTG GTGAAACTCT 
0181 CCGAAAAGAA CTCGACCAAC GAGAATTACC TGCTGATCAA TAGTCACTAC GATTCGGTGC 
0241 CCGGAAGTCC AGGAGCTGGC GATGATGGTT CCATGGTCGT TACCATGTTG GAGGTGATGC 
0301 GTGTGATAGC CAAATCTGGT GATCCACTGG CCCATCCCAT TGTCTTTCTG TTCAATGGAG 
0361 CTGAGGAGAA TCCTCTGCAG GCATCACATG CCTTCATTAC CCAACACAAA TGGGCCAAGA 
0421 ACTGCAAAGC TCTCATCAAC TTGGACTCAG CCGGATCTGG TGGCCGGGAG ATTCTCTTCC 
0481 AGTCGGGACC GAATCATCCG T

in silico PCR Fragment

0001 CAGCATGGCC AACGA-GGAG AGCGCCGTGG AGTTCCTGCG CGCCGAGGTA GCCAAAGTGG 
0061 AGTCCGAAAT GAGCGACCTT CTGGAGATCG AAGTGGATGT GCAGCAGGCC AGTGGAGCCT 
0121 ACATGCACTG GGAGATGGTC AACATGTATC AGGGCATTCA GAATGTGGTG GTGAAACTCT 
0181 CCGAAAAGAA CTCGACCAAC GAGAATTACC TGCTGATCAA TAGTCACTAC GATTCGGTGC 
0241 CCGGAAGTCC AGGAGCTGGC GATGATGGTT CCATGGTCGT TACCATGTTG GAGGTGATGC 
0301 GTGTGATAGC CAAATCTGGT GATCCACTGG CCCATCCCAT TGTCTTTCTG TTCAATGGAG 
0361 CTGAGGAGAA TCCTCTGCAG GCATCACATG CCTTCATTAC CCAACACAAA TGGGCCAAGA 
0421 ACTGCAAAGC TCTCATCAAC TTGGACTCAG CCGGATCTGG TGGCCGGGAG ATTCTCTTCC 
0481 AGTCGGGACC GAATCATCCG T

Assemble Data


10062R-2.IR_full       1   CAGCATGGCCAACGAGGGAGAGCGCCGTGGAGTTCCTGCGCGCCGAGGTAGCCAAAGTGG 60
                           ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
10062R-2.in silico     1   CAGCATGGCCAACGA-GGAGAGCGCCGTGGAGTTCCTGCGCGCCGAGGTAGCCAAAGTGG 60


10062R-2.IR_full       61  AGTCCGAAATGAGCGACCTTCTGGAGATCGAAGTGGATGTGCAGCAGGCCAGTGGAGCCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10062R-2.in silico     61  AGTCCGAAATGAGCGACCTTCTGGAGATCGAAGTGGATGTGCAGCAGGCCAGTGGAGCCT 120


10062R-2.IR_full       121 ACATGCACTGGGAGATGGTCAACATGTATCAGGGCATTCAGAATGTGGTGGTGAAACTCT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10062R-2.in silico     121 ACATGCACTGGGAGATGGTCAACATGTATCAGGGCATTCAGAATGTGGTGGTGAAACTCT 180


10062R-2.IR_full       181 CCGAAAAGAACTCGACCAACGAGAATTACCTGCTGATCAATAGTCACTACGATTCGGTGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10062R-2.in silico     181 CCGAAAAGAACTCGACCAACGAGAATTACCTGCTGATCAATAGTCACTACGATTCGGTGC 240


10062R-2.IR_full       241 CCGGAAGTCCAGGAGCTGGCGATGATGGTTCCATGGTCGTTACCATGTTGGAGGTGATGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10062R-2.in silico     241 CCGGAAGTCCAGGAGCTGGCGATGATGGTTCCATGGTCGTTACCATGTTGGAGGTGATGC 300


10062R-2.IR_full       301 GTGTGATAGCCAAATCTGGTGATCCACTGGCCCATCCCATTGTCTTTCTGTTCAATGGAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10062R-2.in silico     301 GTGTGATAGCCAAATCTGGTGATCCACTGGCCCATCCCATTGTCTTTCTGTTCAATGGAG 360


10062R-2.IR_full       361 CTGAGGAGAATCCTCTGCAGGCATCACATGCCTTCATTACCCAACACAAATGGGCCAAGA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10062R-2.in silico     361 CTGAGGAGAATCCTCTGCAGGCATCACATGCCTTCATTACCCAACACAAATGGGCCAAGA 420


10062R-2.IR_full       421 ACTGCAAAGCTCTCATCAACTTGGACTCAGCCGGATCTGGTGGCCGGGAGATTCTCTTCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10062R-2.in silico     421 ACTGCAAAGCTCTCATCAACTTGGACTCAGCCGGATCTGGTGGCCGGGAGATTCTCTTCC 480


10062R-2.IR_full       481 AGTCGGGACCGAATCATCCGT 501
                           |||||||||||||||||||||
10062R-2.in silico     481 AGTCGGGACCGAATCATCCGT 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_137572.2	CG10062-RA (CG10062), mRNA
1.24	6	8	23	38	NM_137573.2	CG10073-RA (CG10073), mRNA
1.03	5	9	31	37	NM_165888.2	CG30049-RA (CG30049), mRNA
0.41	2	13	25	23	NM_165889.1	CG30043-RA (CG30043), mRNA
0.2	1	11	25	16	NM_137571.1	CG9416-RA (CG9416), mRNA
0.2	1	5	3	11	NM_166125.2	CG18255-RA, transcript variant A (Strn-Mlck), mRNA
0.2	1	5	3	10	NM_166129.2	CG18255-RD, transcript variant D (Strn-Mlck), mRNA
0.2	1	2	7	3	NM_140083.1	CG18179-RA (CG18179), mRNA
0	0	12	37	39	NM_165887.1	CG30047-RA (CG30047), mRNA
0	0	6	37	76	NM_137569.3	CG11961-RB, transcript variant B (CG11961), mRNA
0	0	6	37	76	NM_166342.1	CG11961-RA, transcript variant A (CG11961), mRNA
0	0	5	14	52	NM_176154.1	CG33012-RA, transcript variant A (CG33012), mRNA
0	0	5	14	27	NM_176155.1	CG33012-RB, transcript variant B (CG33012), mRNA
0	0	3	3	19	NM_165890.2	CG13160-RA (CG13160), mRNA
0	0	3	3	2	NM_165196.1	CG31739-RA (CG31739), mRNA
0	0	2	22	43	NM_137574.1	CG10081-RA (CG10081), mRNA
0	0	2	15	39	NM_137570.1	CG10051-RA (CG10051), mRNA
0	0	2	3	9	NM_142538.1	CG5237-RA (CG5237), mRNA
0	0	2	3	6	NM_136912.2	CG13164-RA, transcript variant A (SIP2), mRNA
0	0	2	3	6	NM_165873.2	CG13164-RC, transcript variant C (SIP2), mRNA
0	0	2	3	6	NM_165874.1	CG13164-RE, transcript variant E (SIP2), mRNA
0	0	2	3	6	NM_176152.1	CG13164-RG, transcript variant G (SIP2), mRNA
0	0	1	7	3	NM_132607.1	CG4661-RA (CG4661), mRNA
0	0	1	6	9	NM_139388.1	CG13930-RA (CG13930), mRNA
0	0	1	5	6	NM_206091.1	CG7734-RD, transcript variant D (shn), mRNA
0	0	1	5	6	NM_057376.2	CG7734-RC, transcript variant C (shn), mRNA
0	0	1	5	6	NM_134279.2	CG7734-RB, transcript variant B (shn), mRNA
0	0	1	5	6	NM_057375.3	CG7734-RA, transcript variant A (shn), mRNA
0	0	1	4	9	NM_001031863.1	CG33950-RD, transcript variant D (trol), mRNA
0	0	1	4	9	NM_001031866.1	CG33950-RA, transcript variant A (trol), mRNA

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