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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10059R-2 |
| Symbol |
MAGE |
Full Name |
MAGE |
| CG No |
CG10059 |
Old CG No |
CG10059 |
| Synonyms |
CG10059, MAGE |
| Accession No (Link to NCBI) |
NM_141445.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACATCCTTGATCATACCGCCC |
| Primer Seq. 3' |
aatctagaggtaccTTGCTCGAATGTGAACTCCGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACATCCTTGA TCATACCGCC CAGAAGATTC CCATAAAGGA CAAGGATTTG ATAGCGGTGG
0061 CTGGGGACAA GAGCGAACTG AAGAANACGC CTGCCGCTGG TCACTAATCT GCTGGCCGAA
0121 ACATTCGGTA TAATACTAAC CCCACTGGAC GCGACCACCA AGACGTTCAT CTGCACTGCG
0181 GAGGAGCCGG TGGCCTCCAT TNCANCGAGC TGNACGCCGG CTCAACGGCC GCAGTTCACG
0241 CTGCTGTATA TTATTCTCAT GTACATCTTN CCTGCGCGGC AACCGCATCG AGGACTCGAA
0301 ACTGTACGTT ATGCTGGAGA TGCTCAACAT CTATCCAGAC GAAGAACATG GCTACTTCGG
0361 CCCCAATCTG CGCAAACAGA TCGAGGAGAC GTTTGTGAAG CAACAATATC TGAAGCGGGA
0421 ACGCTCACAG CTAAGTGCTT ACGATGATTC CAAGACTTTC TTCCTTTGGG GACCACGCGC
0481 CAAGGCGGAG TTCACATTCG AGCAA |
| in silico PCR Fragment |
0001 ACATCCTTGA TCATACCGCC CAGAAGATTC CCATAAAGGA CAAGGATTTG ATAGCGGTGG
0061 CTGGGGACAA GAGCGAACTG AAGAA-ACGC CTGCCGCTGG TCACTAATCT GCTGGCCGAA
0121 ACATTCGGTA TAATACTAAC CCCACTGGAC GCGACCACCA AGACGTTCAT CTGCACTGCG
0181 GAGGAGCCGG TGGCCTCCAT T-CA-CGAGC TG-ACGCCGG CTCAACGGCC GCAGTTCACG
0241 CTGCTGTATA TTATTCTCAT GTACATCTT- CCTGCGCGGC AACCGCATCG AGGACTCGAA
0301 ACTGTACGTT ATGCTGGAGA TGCTCAACAT CTATCCAGAC GAAGAACATG GCTACTTCGG
0361 CCCCAATCTG CGCAAACAGA TCGAGGAGAC GTTTGTGAAG CAACAATATC TGAAGCGGGA
0421 ACGCTCACAG CTAAGTGCTT ACGATGATTC CAAGACTTTC TTCCTTTGGG GACCACGTGC
0481 CAAGGCGGAG TTCACATTCG AGCAA |
| Assemble Data |
<PRE>
10059R-2.IR_full 1 ACATCCTTGATCATACCGCCCAGAAGATTCCCATAAAGGACAAGGATTTGATAGCGGTGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10059R-2.in silico 1 ACATCCTTGATCATACCGCCCAGAAGATTCCCATAAAGGACAAGGATTTGATAGCGGTGG 60
10059R-2.IR_full 61 CTGGGGACAAGAGCGAACTGAAGAA<span class="snp"><tt>N</tt></span>ACGCCTGCCGCTGGTCACTAATCTGCTGGCCGAA 120
|||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||
10059R-2.in silico 61 CTGGGGACAAGAGCGAACTGAAGAA<span class="snp"><tt>-</tt></span>ACGCCTGCCGCTGGTCACTAATCTGCTGGCCGAA 120
10059R-2.IR_full 121 ACATTCGGTATAATACTAACCCCACTGGACGCGACCACCAAGACGTTCATCTGCACTGCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10059R-2.in silico 121 ACATTCGGTATAATACTAACCCCACTGGACGCGACCACCAAGACGTTCATCTGCACTGCG 180
10059R-2.IR_full 181 GAGGAGCCGGTGGCCTCCATT<span class="snp"><tt>N</tt></span>CA<span class="snp"><tt>N</tt></span>CGAGCTG<span class="snp"><tt>N</tt></span>ACGCCGGCTCAACGGCCGCAGTTCACG 240
|||||||||||||||||||||<span class="snp"><tt> </tt></span>||<span class="snp"><tt> </tt></span>|||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||
10059R-2.in silico 181 GAGGAGCCGGTGGCCTCCATT<span class="snp"><tt>-</tt></span>CA<span class="snp"><tt>-</tt></span>CGAGCTG<span class="snp"><tt>-</tt></span>ACGCCGGCTCAACGGCCGCAGTTCACG 240
10059R-2.IR_full 241 CTGCTGTATATTATTCTCATGTACATCTT<span class="snp"><tt>N</tt></span>CCTGCGCGGCAACCGCATCGAGGACTCGAA 300
|||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||
10059R-2.in silico 241 CTGCTGTATATTATTCTCATGTACATCTT<span class="snp"><tt>-</tt></span>CCTGCGCGGCAACCGCATCGAGGACTCGAA 300
10059R-2.IR_full 301 ACTGTACGTTATGCTGGAGATGCTCAACATCTATCCAGACGAAGAACATGGCTACTTCGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10059R-2.in silico 301 ACTGTACGTTATGCTGGAGATGCTCAACATCTATCCAGACGAAGAACATGGCTACTTCGG 360
10059R-2.IR_full 361 CCCCAATCTGCGCAAACAGATCGAGGAGACGTTTGTGAAGCAACAATATCTGAAGCGGGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10059R-2.in silico 361 CCCCAATCTGCGCAAACAGATCGAGGAGACGTTTGTGAAGCAACAATATCTGAAGCGGGA 420
10059R-2.IR_full 421 ACGCTCACAGCTAAGTGCTTACGATGATTCCAAGACTTTCTTCCTTTGGGGACCACG<span class="snp"><tt>C</tt></span>GC 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||
10059R-2.in silico 421 ACGCTCACAGCTAAGTGCTTACGATGATTCCAAGACTTTCTTCCTTTGGGGACCACG<span class="snp"><tt>T</tt></span>GC 480
10059R-2.IR_full 481 CAAGGCGGAGTTCACATTCGAGCAA 505
|||||||||||||||||||||||||
10059R-2.in silico 481 CAAGGCGGAGTTCACATTCGAGCAA 505
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_141445.2 |
CG10059-RA (MAGE), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_134741.2 |
CG4947-RA (Tgt), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_138105.1 |
CG13594-RA (CG13594), mRNA |
| 0 |
0 |
2 |
6 |
12 |
NM_078582.3 |
CG32659-RA (Ten-a), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_133104.2 |
CG7282-RA (CG7282), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_169654.2 |
CG31302-RC, transcript variant C (CG31302), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_169652.2 |
CG31302-RA, transcript variant A (CG31302), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_169653.2 |
CG31302-RB, transcript variant B (CG31302), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_080193.3 |
CG5504-RA, transcript variant A (l(2)tid), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_206210.1 |
CG5504-RB, transcript variant B (l(2)tid), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_206209.1 |
CG5504-RC, transcript variant C (l(2)tid), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139805.1 |
CG10077-RA, transcript variant A (CG10077), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169560.1 |
CG2988-RA (ems), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_145757.2 |
CG30084-RC, transcript variant C (CG30084), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140943.2 |
CG32227-RA (CG32227), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140761.2 |
CG7441-RA (CG7441), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_143104.1 |
CG11856-RA (Nup358), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001015251.1 |
CG40460-PB.3 (CG40460), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001015249.1 |
CG40460-PH.3 (CG40460), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001015248.1 |
CG40460-PE.3 (CG40460), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001015246.1 |
CG40460-PI.3 (CG40460), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001015250.1 |
CG40460-PK.3 (CG40460), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001015252.1 |
CG40460-PG.3 (CG40460), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001015247.1 |
CG40460-PJ.3 (CG40460), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001015243.1 |
CG40460-PC.3 (CG40460), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001015245.1 |
CG40460-PF.3 (CG40460), partial mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001015244.1 |
CG40460-PD.3 (CG40460), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_132468.2 |
CG1394-RA (CG1394), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_139461.1 |
CG15877-RA (CG15877), mRNA |
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| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| ++ |
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| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
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| VB |
 |
| |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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