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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10059R-1

Symbol

MAGE

Full Name

MAGE

CG No

CG10059

Old CG No

CG10059

Synonyms

CG10059, MAGE

Accession No (Link to NCBI)

NM_141445.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACATCCTTGATCATACCGCCC

Primer Seq. 3'

aatctagaggtaccTTGCTCGAATGTGAACTCCGC

Predicted Fragment Size

IR fragment full Seq

0001 ACATCCTTGA TCATACCGCC CAGAAGATTC CCATAAAGGA CAAGGATTTG ATAGCGGTGG 
0061 CTGGGGACAA GAGCGAACTG AAGAANACGC CTGCCGCTGG TCACTAATCT GCTGGCCGAA 
0121 ACATTCGGTA TAATACTAAC CCCACTGGAC GCGACCACCA AGACGTTCAT CTGCACTGCG 
0181 GAGGAGCCGG TGGCCTCCAT TNCANCGAGC TGNACGCCGG CTCAACGGCC GCAGTTCACG 
0241 CTGCTGTATA TTATTCTCAT GTACATCTTN CCTGCGCGGC AACCGCATCG AGGACTCGAA 
0301 ACTGTACGTT ATGCTGGAGA TGCTCAACAT CTATCCAGAC GAAGAACATG GCTACTTCGG 
0361 CCCCAATCTG CGCAAACAGA TCGAGGAGAC GTTTGTGAAG CAACAATATC TGAAGCGGGA 
0421 ACGCTCACAG CTAAGTGCTT ACGATGATTC CAAGACTTTC TTCCTTTGGG GACCACGCGC 
0481 CAAGGCGGAG TTCACATTCG AGCAA

in silico PCR Fragment

0001 ACATCCTTGA TCATACCGCC CAGAAGATTC CCATAAAGGA CAAGGATTTG ATAGCGGTGG 
0061 CTGGGGACAA GAGCGAACTG AAGAA-ACGC CTGCCGCTGG TCACTAATCT GCTGGCCGAA 
0121 ACATTCGGTA TAATACTAAC CCCACTGGAC GCGACCACCA AGACGTTCAT CTGCACTGCG 
0181 GAGGAGCCGG TGGCCTCCAT T-CA-CGAGC TG-ACGCCGG CTCAACGGCC GCAGTTCACG 
0241 CTGCTGTATA TTATTCTCAT GTACATCTT- CCTGCGCGGC AACCGCATCG AGGACTCGAA 
0301 ACTGTACGTT ATGCTGGAGA TGCTCAACAT CTATCCAGAC GAAGAACATG GCTACTTCGG 
0361 CCCCAATCTG CGCAAACAGA TCGAGGAGAC GTTTGTGAAG CAACAATATC TGAAGCGGGA 
0421 ACGCTCACAG CTAAGTGCTT ACGATGATTC CAAGACTTTC TTCCTTTGGG GACCACGTGC 
0481 CAAGGCGGAG TTCACATTCG AGCAA

Assemble Data

<PRE>

10059R-1.IR_full       1   ACATCCTTGATCATACCGCCCAGAAGATTCCCATAAAGGACAAGGATTTGATAGCGGTGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10059R-1.in silico     1   ACATCCTTGATCATACCGCCCAGAAGATTCCCATAAAGGACAAGGATTTGATAGCGGTGG 60


10059R-1.IR_full       61  CTGGGGACAAGAGCGAACTGAAGAA<span class="snp"><tt>N</tt></span>ACGCCTGCCGCTGGTCACTAATCTGCTGGCCGAA 120
                           |||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||
10059R-1.in silico     61  CTGGGGACAAGAGCGAACTGAAGAA<span class="snp"><tt>-</tt></span>ACGCCTGCCGCTGGTCACTAATCTGCTGGCCGAA 120


10059R-1.IR_full       121 ACATTCGGTATAATACTAACCCCACTGGACGCGACCACCAAGACGTTCATCTGCACTGCG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10059R-1.in silico     121 ACATTCGGTATAATACTAACCCCACTGGACGCGACCACCAAGACGTTCATCTGCACTGCG 180


10059R-1.IR_full       181 GAGGAGCCGGTGGCCTCCATT<span class="snp"><tt>N</tt></span>CA<span class="snp"><tt>N</tt></span>CGAGCTG<span class="snp"><tt>N</tt></span>ACGCCGGCTCAACGGCCGCAGTTCACG 240
                           |||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>|||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||
10059R-1.in silico     181 GAGGAGCCGGTGGCCTCCATT<span class="snp"><tt>-</tt></span>CA<span class="snp"><tt>-</tt></span>CGAGCTG<span class="snp"><tt>-</tt></span>ACGCCGGCTCAACGGCCGCAGTTCACG 240


10059R-1.IR_full       241 CTGCTGTATATTATTCTCATGTACATCTT<span class="snp"><tt>N</tt></span>CCTGCGCGGCAACCGCATCGAGGACTCGAA 300
                           |||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||
10059R-1.in silico     241 CTGCTGTATATTATTCTCATGTACATCTT<span class="snp"><tt>-</tt></span>CCTGCGCGGCAACCGCATCGAGGACTCGAA 300


10059R-1.IR_full       301 ACTGTACGTTATGCTGGAGATGCTCAACATCTATCCAGACGAAGAACATGGCTACTTCGG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10059R-1.in silico     301 ACTGTACGTTATGCTGGAGATGCTCAACATCTATCCAGACGAAGAACATGGCTACTTCGG 360


10059R-1.IR_full       361 CCCCAATCTGCGCAAACAGATCGAGGAGACGTTTGTGAAGCAACAATATCTGAAGCGGGA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10059R-1.in silico     361 CCCCAATCTGCGCAAACAGATCGAGGAGACGTTTGTGAAGCAACAATATCTGAAGCGGGA 420


10059R-1.IR_full       421 ACGCTCACAGCTAAGTGCTTACGATGATTCCAAGACTTTCTTCCTTTGGGGACCACG<span class="snp"><tt>C</tt></span>GC 480
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||
10059R-1.in silico     421 ACGCTCACAGCTAAGTGCTTACGATGATTCCAAGACTTTCTTCCTTTGGGGACCACG<span class="snp"><tt>T</tt></span>GC 480


10059R-1.IR_full       481 CAAGGCGGAGTTCACATTCGAGCAA 505
                           |||||||||||||||||||||||||
10059R-1.in silico     481 CAAGGCGGAGTTCACATTCGAGCAA 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_141445.2	CG10059-RA (MAGE), mRNA
0	0	4	2	2	NM_134741.2	CG4947-RA (Tgt), mRNA
0	0	3	3	3	NM_138105.1	CG13594-RA (CG13594), mRNA
0	0	2	6	12	NM_078582.3	CG32659-RA (Ten-a), mRNA
0	0	2	2	5	NM_133104.2	CG7282-RA (CG7282), mRNA
0	0	1	6	3	NM_132457.1	CG11122-RA (CG11122), mRNA
0	0	1	4	2	NM_169654.2	CG31302-RC, transcript variant C (CG31302), mRNA
0	0	1	4	2	NM_169652.2	CG31302-RA, transcript variant A (CG31302), mRNA
0	0	1	4	2	NM_169653.2	CG31302-RB, transcript variant B (CG31302), mRNA
0	0	1	3	4	NM_080193.3	CG5504-RA, transcript variant A (l(2)tid), mRNA
0	0	1	3	4	NM_206210.1	CG5504-RB, transcript variant B (l(2)tid), mRNA
0	0	1	3	4	NM_206209.1	CG5504-RC, transcript variant C (l(2)tid), mRNA
0	0	1	3	2	NM_139805.1	CG10077-RA, transcript variant A (CG10077), mRNA
0	0	1	2	4	NM_169560.1	CG2988-RA (ems), mRNA
0	0	1	2	3	NM_145757.2	CG30084-RC, transcript variant C (CG30084), mRNA
0	0	1	2	3	NM_140943.2	CG32227-RA (CG32227), mRNA
0	0	1	2	2	NM_140761.2	CG7441-RA (CG7441), mRNA
0	0	1	2	2	NM_143104.1	CG11856-RA (Nup358), mRNA
0	0	0	5	7	NM_001015251.1	CG40460-PB.3 (CG40460), mRNA
0	0	0	5	7	NM_001015249.1	CG40460-PH.3 (CG40460), mRNA
0	0	0	5	7	NM_001015248.1	CG40460-PE.3 (CG40460), mRNA
0	0	0	5	7	NM_001015246.1	CG40460-PI.3 (CG40460), mRNA
0	0	0	5	7	NM_001015250.1	CG40460-PK.3 (CG40460), mRNA
0	0	0	5	7	NM_001015252.1	CG40460-PG.3 (CG40460), mRNA
0	0	0	5	7	NM_001015247.1	CG40460-PJ.3 (CG40460), mRNA
0	0	0	5	7	NM_001015243.1	CG40460-PC.3 (CG40460), mRNA
0	0	0	5	7	NM_001015245.1	CG40460-PF.3 (CG40460), partial mRNA
0	0	0	5	7	NM_001015244.1	CG40460-PD.3 (CG40460), mRNA
0	0	0	5	3	NM_132468.2	CG1394-RA (CG1394), mRNA
0	0	0	5	3	NM_139461.1	CG15877-RA (CG15877), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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