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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10045R-6 |
| Symbol |
GstD1 |
Full Name |
Glutathione S transferase D1 |
| CG No |
CG10045 |
Old CG No |
CG10045 |
| Synonyms |
GST-1, GSTD1, DmGSTD1-1, CG10045, T5, DmGSTD1, gstD1, DmGST1, DmGST-1, Gst, GST1, gst-D1-1, GST, G-S-T, Gst1-1, GST1-1, unnamed, GSt1-1, Gst1, DmGst1, GSTase, Gst1.1, GstD1 |
| Accession No (Link to NCBI) |
NM_079602.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACTTCTACTACCTGCCCGGCT |
| Primer Seq. 3' |
aatctagaggtaccACCTCGAATGTGGACACGGTT |
| Predicted Fragment Size |
499 |
| IR fragment full Seq |
0001 ACTTCTACTA CCTGCCCGGC TCCTCCCCCT GCCGCTCCGT GATCATGACC GCCAAGGCCG
0061 TGGGCGTCGA GCTGAACAAG AAGCTGCTCA ACCTGCAGGC CGGTGAGCAC CCTGAAGCCG
0121 GAGTTCCTGA AGATCAATCC CCAGCACACC ATTCCCACGC TGGTGGACAA CGGATTCGCG
0181 CTGTGGGAGT CCCGCGCCAT CCAGGTGTAT TTGGTGGAGA AGTACGGCAA GACCGACTCC
0241 CTGTACCCTA AGTGCCCCAA GAAGCGCGCC GTGATCAATC AGCGCCTGTA CTTCGACATG
0301 GGAACGCTGT ACCAGAGCTT CGCCAACTAC TACTACCCAC AGGTGTTCGC CAAGGCGCCC
0361 GCCGATCCAG AGGCCTTCAA GAAGATCGAG GCCGCCTTCG AGTTCCTGAA CACCTTCCTG
0421 GAGGGACAGG ACTACGCCGC CGGTGACTCC CTTACCGTAG CCGACANTTG CCCTGGTGGC
0481 AACCGTGTCC ACATTCGAGG T |
| in silico PCR Fragment |
0001 ACTTCTACTA CCTGCCCGGC TCCTCCCCCT GCCGCTCCGT GATCATGACC GCCAAGGCCG
0061 TGGGCGTCGA GCTGAACAAG AAGCTGCTCA ACCTGCAGGC CGGTGAGCA- CCTGAAGCCG
0121 GAGTTCCTGA AGATCAATCC CCAGCACACC ATTCCCACGC TGGTGGACAA CGGATTCGCG
0181 CTGTGGGAGT CCCGCGCCAT CCAGGTGTAT TTGGTGGAGA AGTACGGCAA GACCGACTCC
0241 CTGTACCCTA AGTGCCCCAA GAAGCGCGCC GTGATCAATC AGCGCCTGTA CTTCGACATG
0301 GGAACGCTGT ACCAGAGCTT CGCCAACTAC TACTACCCAC AGGTGTTCGC CAAGGCGCCC
0361 GCCGATCCAG AGGCCTTCAA GAAGATCGAG GCCGCCTTCG AGTTCCTGAA CACCTTCCTG
0421 GAGGGACAGG ACTACGCCGC CGGTGACTCC CTTACCGTAG CCGACA-TTG CCCTGGTGGC
0481 AACCGTGTCC ACATTCGAGG T |
| Assemble Data |
10045R-6.IR_full 1 ACTTCTACTACCTGCCCGGCTCCTCCCCCTGCCGCTCCGTGATCATGACCGCCAAGGCCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10045R-6.in silico 1 ACTTCTACTACCTGCCCGGCTCCTCCCCCTGCCGCTCCGTGATCATGACCGCCAAGGCCG 60
10045R-6.IR_full 61 TGGGCGTCGAGCTGAACAAGAAGCTGCTCAACCTGCAGGCCGGTGAGCACCCTGAAGCCG 120
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
10045R-6.in silico 61 TGGGCGTCGAGCTGAACAAGAAGCTGCTCAACCTGCAGGCCGGTGAGCA-CCTGAAGCCG 120
10045R-6.IR_full 121 GAGTTCCTGAAGATCAATCCCCAGCACACCATTCCCACGCTGGTGGACAACGGATTCGCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10045R-6.in silico 121 GAGTTCCTGAAGATCAATCCCCAGCACACCATTCCCACGCTGGTGGACAACGGATTCGCG 180
10045R-6.IR_full 181 CTGTGGGAGTCCCGCGCCATCCAGGTGTATTTGGTGGAGAAGTACGGCAAGACCGACTCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10045R-6.in silico 181 CTGTGGGAGTCCCGCGCCATCCAGGTGTATTTGGTGGAGAAGTACGGCAAGACCGACTCC 240
10045R-6.IR_full 241 CTGTACCCTAAGTGCCCCAAGAAGCGCGCCGTGATCAATCAGCGCCTGTACTTCGACATG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10045R-6.in silico 241 CTGTACCCTAAGTGCCCCAAGAAGCGCGCCGTGATCAATCAGCGCCTGTACTTCGACATG 300
10045R-6.IR_full 301 GGAACGCTGTACCAGAGCTTCGCCAACTACTACTACCCACAGGTGTTCGCCAAGGCGCCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10045R-6.in silico 301 GGAACGCTGTACCAGAGCTTCGCCAACTACTACTACCCACAGGTGTTCGCCAAGGCGCCC 360
10045R-6.IR_full 361 GCCGATCCAGAGGCCTTCAAGAAGATCGAGGCCGCCTTCGAGTTCCTGAACACCTTCCTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10045R-6.in silico 361 GCCGATCCAGAGGCCTTCAAGAAGATCGAGGCCGCCTTCGAGTTCCTGAACACCTTCCTG 420
10045R-6.IR_full 421 GAGGGACAGGACTACGCCGCCGGTGACTCCCTTACCGTAGCCGACANTTGCCCTGGTGGC 480
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
10045R-6.in silico 421 GAGGGACAGGACTACGCCGCCGGTGACTCCCTTACCGTAGCCGACA-TTGCCCTGGTGGC 480
10045R-6.IR_full 481 AACCGTGTCCACATTCGAGGT 501
|||||||||||||||||||||
10045R-6.in silico 481 AACCGTGTCCACATTCGAGGT 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
481 |
0 |
0 |
0 |
NM_001038953.1 |
CG10045-RB, transcript variant B (GstD1), mRNA |
| 100 |
481 |
0 |
0 |
0 |
NM_079602.3 |
CG10045-RA, transcript variant A (GstD1), mRNA |
| 4.57 |
22 |
40 |
50 |
50 |
NM_080173.1 |
CG4181-RA (GstD2), mRNA |
| 3.95 |
19 |
22 |
40 |
78 |
NM_080175.2 |
CG12242-RA (GstD5), mRNA |
| 3.95 |
19 |
16 |
37 |
48 |
NM_080174.2 |
CG11512-RA (GstD4), mRNA |
| 3.11 |
15 |
15 |
34 |
65 |
NM_144456.1 |
CG18548-RA (GstD10), mRNA |
| 2.49 |
12 |
26 |
19 |
40 |
NM_080375.1 |
CG4371-RA (GstD7), mRNA |
| 0.83 |
4 |
3 |
28 |
50 |
NM_141924.1 |
CG10091-RA (GstD9), mRNA |
| 0 |
0 |
14 |
39 |
90 |
NM_080177.1 |
CG4421-RA (GstD8), mRNA |
| 0 |
0 |
12 |
18 |
7 |
NM_137481.2 |
CG17524-RA (GstE3), mRNA |
| 0 |
0 |
8 |
43 |
45 |
NM_176479.1 |
CG4381-RA (GstD3), mRNA |
| 0 |
0 |
4 |
4 |
7 |
NM_001043070.1 |
CG34146-RA (brp), mRNA |
| 0 |
0 |
3 |
4 |
14 |
NM_166279.2 |
CG17534-RA (GstE9), mRNA |
| 0 |
0 |
3 |
4 |
6 |
NM_057629.3 |
CG4379-RA, transcript variant A (Pka-C1), mRNA |
| 0 |
0 |
3 |
4 |
6 |
NM_164866.1 |
CG4379-RB, transcript variant B (Pka-C1), mRNA |
| 0 |
0 |
3 |
4 |
6 |
NM_205950.1 |
CG4379-RC, transcript variant C (Pka-C1), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_141142.1 |
CG6914-RA (CG6914), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_143323.3 |
CG12880-RA (CG12880), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_143666.2 |
CG2009-RA (bip2), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_134701.2 |
CG3883-RA (CG3883), mRNA |
| 0 |
0 |
2 |
3 |
9 |
NM_137061.2 |
CG6337-RA (CG6337), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_141886.2 |
CG3571-RA, transcript variant A (CG3571), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_169446.1 |
CG3571-RB, transcript variant B (CG3571), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_165802.1 |
CG30015-RA, transcript variant A (CG30015), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_079935.2 |
CG8094-RA (Hex-C), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_136777.2 |
CG30015-RB, transcript variant B (CG30015), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132719.2 |
CG32604-RA, transcript variant A (l(1)G0007), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167396.1 |
CG32604-RB, transcript variant B (l(1)G0007), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_079113.2 |
CG4012-RA (gek), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_133075.2 |
CG6461-RA (CG6461), mRNA |
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