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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10036R-1 |
| Symbol |
otp |
Full Name |
orthopedia |
| CG No |
CG10036 |
Old CG No |
CG10036 |
| Synonyms |
Otp, w26, bk24, W26, CG2965, CG10036, BcDNA:RE58095, otp, orthopedia, Orthopedia |
| Accession No (Link to NCBI) |
NM_079075.4 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACTATCCGGACATCTTCATGC |
| Primer Seq. 3' |
aatctagaggtaccTTGGTGAGTTTGCTGTTGCTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACTATCCGGA CATCTTCATG CGCGAGGAGA TCGCCATGCG GATTGGCCTC ACTGAGTCCC
0061 GCGTTCAGGT CTGGTTCCAG AACCGCCGTG CCAAGTGGAA GAAGCGCAAG AAGACAACCA
0121 ATGTCTTCCG CACCCCGGGC GCCCTGCTGC CCTCCCATGG ACTTCCGCCG TTTGGAGCGA
0181 ATATAACTAA TATCGCCATG GGCGATGGTC TCTGTGGCAC AGGAATGTTT GGCGGAGATC
0241 GCTGGAGCGT GGGTGTCAAT CCAATGACGG CAGGCGACTC CATGATGTAC CAGCACAGTG
0301 TGGGCGGAGT CAGTTGTGGG CCCAGTGGTT CGCCGAGCGC CACCACCCCG CCGAACATGA
0361 ACAGCTGCTC CTCGGTGACC CCGCCGCCAC TTTCCGCGCA GCCGAACTCC AGCCAGAACG
0421 AGCTGAACGG CGAGCCCATG CCGCTGCATC AGCAGCAACA ACAACAGACT CACCAACATC
0481 AGCAACAGCA AACTCACCAA |
| in silico PCR Fragment |
0001 ACTATCCGGA CATCTTCATG CGCGAGGAGA TCGCCATGCG GATTGGCCTC ACTGAGTCCC
0061 GCGTTCAGGT CTGGTTCCAG AACCGCCGTG CCAAGTGGAA GAAGCGCAAG AAGACAACCA
0121 ATGTCTTCCG CACCCCGGGC GCCCTGCTGC CCTCCCATGG ACTTCCGCCG TTTGGGGCCA
0181 ATATAACCAA TATCGCCATG GGCGATGGTC TCTGTGGCAC GGGAATGTTT GGCGGAGATC
0241 GCTGGAGCGT GGGTGTCAAT CCAATGACGG CAGGCGACTC CATGATGTAC CAGCACAGTG
0301 TGGGCGGAGT CAGTTGTGGG CCCAGTGGTT CGCCGAGCGC CACCACCCCG CCGAACATGA
0361 ACAGCTGCTC CTCGGTGACC CCGCCGCCAC TTTCCGCGCA GCCGAACTCC AGCCAAAACG
0421 AGCTGAACGG CGAGCCCATG CCGCTGCACC AGCAGCAACA ACAACAGACT CACCAACATC
0481 AGCAACAGCA AACTCACCAA |
| Assemble Data |
10036R-1.IR_full 1 ACTATCCGGACATCTTCATGCGCGAGGAGATCGCCATGCGGATTGGCCTCACTGAGTCCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10036R-1.in silico 1 ACTATCCGGACATCTTCATGCGCGAGGAGATCGCCATGCGGATTGGCCTCACTGAGTCCC 60
10036R-1.IR_full 61 GCGTTCAGGTCTGGTTCCAGAACCGCCGTGCCAAGTGGAAGAAGCGCAAGAAGACAACCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10036R-1.in silico 61 GCGTTCAGGTCTGGTTCCAGAACCGCCGTGCCAAGTGGAAGAAGCGCAAGAAGACAACCA 120
10036R-1.IR_full 121 ATGTCTTCCGCACCCCGGGCGCCCTGCTGCCCTCCCATGGACTTCCGCCGTTTGGAGCGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||| || |
10036R-1.in silico 121 ATGTCTTCCGCACCCCGGGCGCCCTGCTGCCCTCCCATGGACTTCCGCCGTTTGGGGCCA 180
10036R-1.IR_full 181 ATATAACTAATATCGCCATGGGCGATGGTCTCTGTGGCACAGGAATGTTTGGCGGAGATC 240
||||||| |||||||||||||||||||||||||||||||| |||||||||||||||||||
10036R-1.in silico 181 ATATAACCAATATCGCCATGGGCGATGGTCTCTGTGGCACGGGAATGTTTGGCGGAGATC 240
10036R-1.IR_full 241 GCTGGAGCGTGGGTGTCAATCCAATGACGGCAGGCGACTCCATGATGTACCAGCACAGTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10036R-1.in silico 241 GCTGGAGCGTGGGTGTCAATCCAATGACGGCAGGCGACTCCATGATGTACCAGCACAGTG 300
10036R-1.IR_full 301 TGGGCGGAGTCAGTTGTGGGCCCAGTGGTTCGCCGAGCGCCACCACCCCGCCGAACATGA 360
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10036R-1.in silico 301 TGGGCGGAGTCAGTTGTGGGCCCAGTGGTTCGCCGAGCGCCACCACCCCGCCGAACATGA 360
10036R-1.IR_full 361 ACAGCTGCTCCTCGGTGACCCCGCCGCCACTTTCCGCGCAGCCGAACTCCAGCCAGAACG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
10036R-1.in silico 361 ACAGCTGCTCCTCGGTGACCCCGCCGCCACTTTCCGCGCAGCCGAACTCCAGCCAAAACG 420
10036R-1.IR_full 421 AGCTGAACGGCGAGCCCATGCCGCTGCATCAGCAGCAACAACAACAGACTCACCAACATC 480
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
10036R-1.in silico 421 AGCTGAACGGCGAGCCCATGCCGCTGCACCAGCAGCAACAACAACAGACTCACCAACATC 480
10036R-1.IR_full 481 AGCAACAGCAAACTCACCAA 500
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10036R-1.in silico 481 AGCAACAGCAAACTCACCAA 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
16 |
NM_206187.2 |
CG10036-RB, transcript variant B (otp), mRNA |
| 100 |
482 |
0 |
2 |
16 |
NM_001043098.1 |
CG10036-RC, transcript variant C (otp), mRNA |
| 100 |
482 |
0 |
2 |
16 |
NM_079075.4 |
CG10036-RA, transcript variant A (otp), mRNA |
| 0.82 |
4 |
5 |
7 |
11 |
NM_133014.2 |
CG6269-RA (unc-4), mRNA |
| 0.62 |
3 |
5 |
3 |
3 |
NM_079110.2 |
CG11182-RA (PHDP), mRNA |
| 0.62 |
3 |
3 |
11 |
6 |
NM_164547.1 |
CG2808-RB, transcript variant B (CG2808), mRNA |
| 0.62 |
3 |
2 |
5 |
42 |
NM_133154.2 |
CG12199-RA, transcript variant A (kek5), mRNA |
| 0.62 |
3 |
2 |
5 |
42 |
NM_167647.2 |
CG12199-RB, transcript variant B (kek5), mRNA |
| 0.41 |
2 |
5 |
29 |
116 |
NM_168237.1 |
CG17888-RD, transcript variant D (Pdp1), mRNA |
| 0.41 |
2 |
4 |
6 |
15 |
NM_140568.1 |
CG5830-RA (CG5830), mRNA |
| 0.41 |
2 |
3 |
18 |
86 |
NM_001014681.1 |
CG33553-RD, transcript variant D (Doa), mRNA |
| 0.41 |
2 |
3 |
18 |
86 |
NM_001043298.1 |
CG33553-RH, transcript variant H (Doa), mRNA |
| 0.41 |
2 |
3 |
18 |
83 |
NM_001014679.1 |
CG33553-RC, transcript variant C (Doa), mRNA |
| 0.41 |
2 |
3 |
9 |
38 |
NM_079940.4 |
CG16973-RA, transcript variant A (msn), mRNA |
| 0.41 |
2 |
3 |
9 |
27 |
NM_206250.2 |
CG16973-RC, transcript variant C (msn), mRNA |
| 0.41 |
2 |
3 |
9 |
27 |
NM_206252.2 |
CG16973-RB, transcript variant B (msn), mRNA |
| 0.41 |
2 |
3 |
9 |
27 |
NM_206251.2 |
CG16973-RD, transcript variant D (msn), mRNA |
| 0.41 |
2 |
3 |
9 |
27 |
NM_206249.2 |
CG16973-RE, transcript variant E (msn), mRNA |
| 0.2 |
1 |
10 |
5 |
18 |
NM_057606.4 |
CG1759-RA, transcript variant A (cad), mRNA |
| 0.2 |
1 |
10 |
5 |
18 |
NM_134301.3 |
CG1759-RB, transcript variant B (cad), mRNA |
| 0.2 |
1 |
9 |
16 |
43 |
NM_140800.2 |
CG6884-RA (MED11), mRNA |
| 0.2 |
1 |
9 |
4 |
22 |
NM_136503.2 |
CG12769-RA, transcript variant A (CG12769), mRNA |
| 0.2 |
1 |
9 |
4 |
21 |
NM_165585.1 |
CG12769-RB, transcript variant B (CG12769), mRNA |
| 0.2 |
1 |
6 |
27 |
90 |
NM_057489.3 |
CG4722-RA (bib), mRNA |
| 0.2 |
1 |
5 |
4 |
14 |
NM_169293.1 |
CG8208-RA, transcript variant A (MBD-like), mRNA |
| 0.2 |
1 |
5 |
4 |
12 |
NM_141650.2 |
CG8208-RB, transcript variant B (MBD-like), mRNA |
| 0.2 |
1 |
4 |
3 |
11 |
NM_164743.1 |
CG31906-RA (CG31906), mRNA |
| 0.2 |
1 |
2 |
11 |
31 |
NM_057265.3 |
CG1264-RA (lab), mRNA |
| 0 |
0 |
22 |
107 |
358 |
NM_168571.2 |
CG32133-RA (CG32133), mRNA |
| 0 |
0 |
22 |
83 |
256 |
NM_135077.2 |
CG14023-RA (CG14023), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
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| ++ |
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| DB |
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| + |
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| DC |
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| ++ |
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| DM |
 |
| + |
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| VM |
 |
| + |
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| VB |
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