NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10055R-2

Symbol CG10055 Full Name CG10055

CG No CG10055 Old CG No CG10055

Synonyms RE52393, CG10055

Accession No (Link to NCBI) NM_141446.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCAGCTGCTGCTGCTTCTTC

Primer Seq. 3'

aatctagaggtaccTCAGCTTGTAGGTGCGCTCGT

Predicted Fragment Size

IR fragment full Seq

0001 AGCAGCTGCT GCTgCTTCTT CTTCCGCTGG TGAGGTGTTT GGTCAGGAAG CGGGGTCGGG 
0061 GNTTGGCGGA AGNGGAGGCG TTGCATGTGG ATCGCATAAG AAACTGCGAT ATATTCGTTG 
0121 ACGCGCACGA TCTTTATTTC TACGCAGATG TTAAGCTGTA CAAATTCCAC AAGCCGTCGC 
0181 ACCTTCGACT TGCGAGAGCT GAAGACGCTG TTGATAAAGT TCAACACGAC AGAGAATCAC 
0241 TACCAAATTT GCCTGCGCTG TCTGCCCCTA ACAGCGGGTG CCCAGGAAGG ACCCGGTGGA 
0301 GAAAGAGGCG GAGACGGTGG TACGGGGGAT GCAGCCAAGC AGTGGAATAG TCGCAAAGAG 
0361 TACATAGCCG CATTCCTGCA CCTACCCACA CTGAGTGCCA ATTTGCCGGA CAAGCAGCCT 
0421 CTGGTGCAGG AATGGAAACT CAAACGAATC ACCGATCAGT GCCAATTGCA GCTCGACGAC 
0481 CACGAGCGCA CCTACAAGCT GA

in silico PCR Fragment

0001 AGCAGCTGCT GCTGCTTCTT CTTCCGCTGG TGAGGAGTTT GGTCAGGAAG CGGGGTCGGG 
0061 G-TTGGCGGA AGCGGAGGCG TTGCATGTGG ATCGCATAAG AAACTGCGAT ATATTCGTTG 
0121 ACGCGCACGA TCTTTATTTC TACGCAGATG TTAAGCTGTA CAAATTCCAC -AGCCGTCGC 
0181 ACCTTCGACT TGCGAGAGCT GAAGACGCTG TTGATAAAGT TCAACACGAC AGAGAATCAC 
0241 TACCAAATTT GCCTGCGCTG TCTGCCCCTA ACAGCGGGTG CCCAGGAAGG ACCCGGTGGA 
0301 GAAAGAGGCG GAGGCGGTGG TACGGGGGAT GCAGCCAAGC AGTGGAATAG TCGCAAAGAG 
0361 TACATAGCCG CATTCCTGCA CCTACCCACA CTGAGTGCCA ATTTGCCGGA CAAGCAGCCT 
0421 CTGGTGCAGG AATGGAAACT CAAACGAATC ACCGATCAGT GCCAATTGCA GCTCGACGAC 
0481 CACGAGCGCA CCTACAAGCT GA

Assemble Data


10055R-2.IR_full       1   AGCAGCTGCTGCTGCTTCTTCTTCCGCTGGTGAGGTGTTTGGTCAGGAAGCGGGGTCGGG 60
                           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
10055R-2.in silico     1   AGCAGCTGCTGCTGCTTCTTCTTCCGCTGGTGAGGAGTTTGGTCAGGAAGCGGGGTCGGG 60


10055R-2.IR_full       61  GNTTGGCGGAAGNGGAGGCGTTGCATGTGGATCGCATAAGAAACTGCGATATATTCGTTG 120
                           | |||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
10055R-2.in silico     61  G-TTGGCGGAAGCGGAGGCGTTGCATGTGGATCGCATAAGAAACTGCGATATATTCGTTG 120


10055R-2.IR_full       121 ACGCGCACGATCTTTATTTCTACGCAGATGTTAAGCTGTACAAATTCCACAAGCCGTCGC 180
                           |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
10055R-2.in silico     121 ACGCGCACGATCTTTATTTCTACGCAGATGTTAAGCTGTACAAATTCCAC-AGCCGTCGC 180


10055R-2.IR_full       181 ACCTTCGACTTGCGAGAGCTGAAGACGCTGTTGATAAAGTTCAACACGACAGAGAATCAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10055R-2.in silico     181 ACCTTCGACTTGCGAGAGCTGAAGACGCTGTTGATAAAGTTCAACACGACAGAGAATCAC 240


10055R-2.IR_full       241 TACCAAATTTGCCTGCGCTGTCTGCCCCTAACAGCGGGTGCCCAGGAAGGACCCGGTGGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10055R-2.in silico     241 TACCAAATTTGCCTGCGCTGTCTGCCCCTAACAGCGGGTGCCCAGGAAGGACCCGGTGGA 300


10055R-2.IR_full       301 GAAAGAGGCGGAGACGGTGGTACGGGGGATGCAGCCAAGCAGTGGAATAGTCGCAAAGAG 360
                           ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
10055R-2.in silico     301 GAAAGAGGCGGAGGCGGTGGTACGGGGGATGCAGCCAAGCAGTGGAATAGTCGCAAAGAG 360


10055R-2.IR_full       361 TACATAGCCGCATTCCTGCACCTACCCACACTGAGTGCCAATTTGCCGGACAAGCAGCCT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10055R-2.in silico     361 TACATAGCCGCATTCCTGCACCTACCCACACTGAGTGCCAATTTGCCGGACAAGCAGCCT 420


10055R-2.IR_full       421 CTGGTGCAGGAATGGAAACTCAAACGAATCACCGATCAGTGCCAATTGCAGCTCGACGAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10055R-2.in silico     421 CTGGTGCAGGAATGGAAACTCAAACGAATCACCGATCAGTGCCAATTGCAGCTCGACGAC 480


10055R-2.IR_full       481 CACGAGCGCACCTACAAGCTGA 502
                           ||||||||||||||||||||||
10055R-2.in silico     481 CACGAGCGCACCTACAAGCTGA 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	9	7	NM_141446.1	CG10055-RA (CG10055), mRNA
0.62	3	5	6	15	NM_142431.1	CG7187-RA, transcript variant A (Ssdp), mRNA
0.62	3	5	6	15	NM_169794.1	CG7187-RB, transcript variant B (Ssdp), mRNA
0.62	3	5	5	13	NM_169795.1	CG7187-RC, transcript variant C (Ssdp), mRNA
0.41	2	5	10	10	NM_167373.1	CG32629-RA (CG32629), mRNA
0.41	2	5	7	10	NM_170155.1	CG31127-RA (Wsck), mRNA
0.41	2	5	4	22	NM_001014537.1	CG15112-RD, transcript variant D (ena), mRNA
0.41	2	5	4	22	NM_166329.1	CG15112-RA, transcript variant A (ena), mRNA
0.41	2	5	4	22	NM_001014536.1	CG15112-RE, transcript variant E (ena), mRNA
0.41	2	5	4	22	NM_166330.1	CG15112-RC, transcript variant C (ena), mRNA
0.41	2	4	8	21	NM_142622.1	CG4360-RA (CG4360), mRNA
0.41	2	3	6	12	NM_132936.2	CG4768-RA (CG4768), mRNA
0.41	2	2	5	8	NM_057632.3	CG10072-RA (sgl), mRNA
0.2	1	6	7	13	NM_168648.1	CG32156-RB, transcript variant B (Mbs), mRNA
0.2	1	6	7	12	NM_168646.1	CG32156-RC, transcript variant C (Mbs), mRNA
0.2	1	4	10	18	NM_080335.2	CG2984-RA (Pp2C1), mRNA
0.2	1	4	9	44	NM_078601.2	CG9533-RA (rut), mRNA
0	0	5	12	48	NM_132959.2	CG8949-RA (CG8949), mRNA
0	0	5	7	21	NM_170350.1	CG12878-RB, transcript variant B (btz), mRNA
0	0	5	5	18	NM_134510.1	CG12703-RA (CG12703), mRNA
0	0	4	3	6	NM_136033.2	CG12750-RA (ncm), mRNA
0	0	3	11	13	NM_167032.1	CG10706-RB, transcript variant B (SK), mRNA
0	0	3	11	13	NM_167031.1	CG10706-RA, transcript variant A (SK), mRNA
0	0	3	11	13	NM_206631.1	CG10706-RF, transcript variant F (SK), mRNA
0	0	3	11	13	NM_167029.1	CG10706-RC, transcript variant C (SK), mRNA
0	0	3	7	14	NM_166992.2	CG2904-RA (ec), mRNA
0	0	3	6	9	NM_133104.2	CG7282-RA (CG7282), mRNA
0	0	3	4	5	NM_001038879.1	CG11303-RB, transcript variant B (TM4SF), mRNA
0	0	3	4	5	NM_057900.3	CG11303-RA, transcript variant A (TM4SF), mRNA
0	0	2	13	18	NM_057309.2	CG6534-RA (slou), mRNA

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