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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10034R-2 |
| Symbol |
tj |
Full Name |
traffic jam |
| CG No |
CG10034 |
Old CG No |
CG10034 |
| Synonyms |
maf-L, CG10034, Dmaf, PL3, maf1, fs(2)eoPL3, fs(2)eo2, fs(2)eo-B, tj |
| Accession No (Link to NCBI) |
NM_136125.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Panchal T, Chen X, Alchits E, Oh Y, Poon J, Kouptsova J, Laski FA, Godt D.
Specification and spatial arrangement of cells in the germline stem cell niche of the Drosophila ovary depend on the Maf transcription factor Traffic jam.
PLoS Genet (2017) 13(5) e1006790
[
PubMed ID = 28542174
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Gunawan F, Arandjelovic M, Godt D.
The Maf factor Traffic jam both enables and inhibits collective cell migration in Drosophila oogenesis.
Development (2013) 140(13) 2808-17
[
PubMed ID = 23720044
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACGACACGTACGTTCAGGAGT |
| Primer Seq. 3' |
aatctagaggtaccGTGGTGTGCGTAAGTCTGAGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACGACACGTA CGTTCAGGAG TTTGATCTGC ACCACCTGGA GGTGGGCGGA AATGGAGCTG
0061 GCCAATCGCA TGGAGCCGGT GCCGCGTCCC AGGTCACGAC TTTGGGCCAT GTGAAGCGCG
0121 AGGATCACAG TCCACCGCAG CCGGTGGCGG TTAAATGGAC GACAATCCAC GGCGATCCCA
0181 CGAATCCGGA CGATGACCCG GACTCGCTGC CGCTGTCCGG ACCAGAGCCC GCCGGAGCAG
0241 TGGAGGTCTC GCCATCGCCG CACATCAAGC TGAGGTCCTT CTCCGGCCAT CACCAGTGGC
0301 ACATGGACGA NCGGCGCCTG CAACCGCTCT CTCCGCCACC AGAGCAGTAT GGTCCGCTGC
0361 CCGGACAGGC GATTCTGGTG AACACATCTT CGGGAGCGGG CGTTCCGGGC GGAGTGCCCT
0421 CAACGCCGCC GGAAACGCCA CCAGTAGTGG GCTCGCCcAC GGGCAGCAGC AGCTGTCCGG
0481 CTCAGACTTA CGCACACCAC |
| in silico PCR Fragment |
0001 ACGACACGTA CGTTCAGGAG TTTGATCTGC ACCACCTGGA GGTGGGCGGA AATGGAGCTG
0061 GCCAATCGCA TGGAGCCGGT GCCGCGTCCC AGGTCACGAC TTTGGGCCAT GTGAAGCGCG
0121 AGGATCACAG TCCACCGCAG CCGGTGGCGG TTAAATGGAC GACAATCCAC GGCGATCCCA
0181 CGAATCCGGA CGATGACCCG GACTCGCTGC CGCTGTCCGG ACCAGAGCCC GCCGGAGCAG
0241 TGGAGGTCTC GCCATCGCCG CACATCAAGC TGAGGTCCTT CTCCGGCCAT CACCAGTGGC
0301 ACATGGACGA ACGGCGCCTG CAACCGCTCT CTCCGCCACC AGAGCAGTAT GGTCCGCTGC
0361 CCGGACAGGC GATTCTGGTG AACACATCTT CGGGAGCGGG CGTTCCGGGC GGAGTGCCCT
0421 CAACGCCGCC GGAAACGCCA CCAGTAGTGG GCTCGCCCAC GGGCAGCAGC AGCTGTCCGG
0481 CTCAGACTTA CGCACACCAC |
| Assemble Data |
10034R-2.IR_full 1 ACGACACGTACGTTCAGGAGTTTGATCTGCACCACCTGGAGGTGGGCGGAAATGGAGCTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10034R-2.in silico 1 ACGACACGTACGTTCAGGAGTTTGATCTGCACCACCTGGAGGTGGGCGGAAATGGAGCTG 60
10034R-2.IR_full 61 GCCAATCGCATGGAGCCGGTGCCGCGTCCCAGGTCACGACTTTGGGCCATGTGAAGCGCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10034R-2.in silico 61 GCCAATCGCATGGAGCCGGTGCCGCGTCCCAGGTCACGACTTTGGGCCATGTGAAGCGCG 120
10034R-2.IR_full 121 AGGATCACAGTCCACCGCAGCCGGTGGCGGTTAAATGGACGACAATCCACGGCGATCCCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10034R-2.in silico 121 AGGATCACAGTCCACCGCAGCCGGTGGCGGTTAAATGGACGACAATCCACGGCGATCCCA 180
10034R-2.IR_full 181 CGAATCCGGACGATGACCCGGACTCGCTGCCGCTGTCCGGACCAGAGCCCGCCGGAGCAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10034R-2.in silico 181 CGAATCCGGACGATGACCCGGACTCGCTGCCGCTGTCCGGACCAGAGCCCGCCGGAGCAG 240
10034R-2.IR_full 241 TGGAGGTCTCGCCATCGCCGCACATCAAGCTGAGGTCCTTCTCCGGCCATCACCAGTGGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10034R-2.in silico 241 TGGAGGTCTCGCCATCGCCGCACATCAAGCTGAGGTCCTTCTCCGGCCATCACCAGTGGC 300
10034R-2.IR_full 301 ACATGGACGANCGGCGCCTGCAACCGCTCTCTCCGCCACCAGAGCAGTATGGTCCGCTGC 360
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
10034R-2.in silico 301 ACATGGACGAACGGCGCCTGCAACCGCTCTCTCCGCCACCAGAGCAGTATGGTCCGCTGC 360
10034R-2.IR_full 361 CCGGACAGGCGATTCTGGTGAACACATCTTCGGGAGCGGGCGTTCCGGGCGGAGTGCCCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10034R-2.in silico 361 CCGGACAGGCGATTCTGGTGAACACATCTTCGGGAGCGGGCGTTCCGGGCGGAGTGCCCT 420
10034R-2.IR_full 421 CAACGCCGCCGGAAACGCCACCAGTAGTGGGCTCGCCCACGGGCAGCAGCAGCTGTCCGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10034R-2.in silico 421 CAACGCCGCCGGAAACGCCACCAGTAGTGGGCTCGCCCACGGGCAGCAGCAGCTGTCCGG 480
10034R-2.IR_full 481 CTCAGACTTACGCACACCAC 500
||||||||||||||||||||
10034R-2.in silico 481 CTCAGACTTACGCACACCAC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
5 |
NM_136125.1 |
CG10034-RA (tj), mRNA |
| 0 |
0 |
4 |
3 |
2 |
NM_142346.1 |
CG5246-RA (CG5246), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_138265.2 |
CG13916-RA (SA-2), mRNA |
| 0 |
0 |
2 |
4 |
10 |
NM_176556.1 |
CG33106-RA, transcript variant A (mask), mRNA |
| 0 |
0 |
2 |
4 |
10 |
NM_176557.1 |
CG33106-RB, transcript variant B (mask), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_132384.2 |
CG2962-RA (CG2962), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_164830.3 |
CG18405-RA, transcript variant A (Sema-1a), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001042789.1 |
CG13366-RB, transcript variant B (CG13366), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_130492.2 |
CG13366-RA, transcript variant A (CG13366), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_057719.3 |
CG8274-RA (Mtor), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_137663.1 |
CG13437-RA (CG13437), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_135057.1 |
CG9121-RA (CG9121), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_136367.1 |
CG30431-RA (CG30431), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167985.1 |
CG14955-RB, transcript variant B (CG14955), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_139512.1 |
CG14955-RA, transcript variant A (CG14955), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_166940.1 |
CG32796-RB, transcript variant B (CG32796), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168473.2 |
CG32088-RA (CG32088), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_167436.1 |
CG6170-RB, transcript variant B (HDAC6), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_167437.1 |
CG6170-RC, transcript variant C (HDAC6), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132789.2 |
CG6170-RA, transcript variant A (HDAC6), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_078977.2 |
CG10897-RA, transcript variant A (tou), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057999.4 |
CG4602-RA (Srp54), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_170105.1 |
CG31451-RA (CG31451), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168670.1 |
CG32158-RB, transcript variant B (CG32158), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166327.1 |
CG15118-RD, transcript variant D (CG15118), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137552.2 |
CG15118-RB, transcript variant B (CG15118), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166325.1 |
CG15118-RA, transcript variant A (CG15118), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166326.1 |
CG15118-RC, transcript variant C (CG15118), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_168019.2 |
CG32279-RA (dro2), mRNA |
| 0 |
0 |
0 |
7 |
13 |
NM_143645.1 |
CG1976-RA (RhoGAP100F), mRNA |
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