NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10501R-1

Symbol amd Full Name alpha methyl dopa-resistant

CG No CG10501 Old CG No CG10501

Synonyms CG10501, X04695, l(2)amd alpha-methyldopa hypersensitive, l(2)amd, fAMD, l(2)amd[H], l(2)37Bk, CG17345, amd

Accession No (Link to NCBI) NM_057244.3

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGATGCCAAGGAGTTTCGGG

Primer Seq. 3'

aatctagaggtaccGTAGTTGGCCACAGCTTGTTC

Predicted Fragment Size

IR fragment full Seq

0001 TGGATGCCAA GGAGTTTCGG GAATTCGGCA AGGCCGCCAT TGACTACATA GCCGACTATC 
0061 TGGAGAATAT TCGGGATGAC GACGTACTGC CCAATGTGGA GCCAGGCTAT CTGTTGGACC 
0121 TGCTGCCCAC AGAGATGCCG GAAGAGCCCG AAGCGTGGAA GGATGTCCTC GGCGACATTA 
0181 GTCGCGTCAT CAAGCCGGGA CTGACCCACT GGCAGTCGCC TCACATGCAT GCCTACTACC 
0241 CCACCAGCAC CTCGTATCCC TCCATTGTGG GCGAGATGCT GGCCAGCGGG TTCGGCGTCA 
0301 TCGGATTCAG CTGGATCTGC AGTCCCGCCT GCACAGAACT GGAGGTGGTG GTCATGGACT 
0361 GGCTGGCCAA GTTCCTGAAG CTGCCCGCAC ACTTCCAGCA CGCCAGCGAT GGACCAGGAG 
0421 GCGGGGTGAT CCAGGGATCA GCTAGCGAGG CTGTGTTGGT GGCTGTCCTA GCTGCCAGGG 
0481 AACAATGCTG TGGCCAACTA C

in silico PCR Fragment

0001 TGGATGCCAA GGAGTTTCGG GAATTCGGCA AGGCCGCCAT TGACTACATA GCCGACTATC 
0061 TGGAGAATAT TCGGGATGAC GACGTACTGC CCAATGTGGA GCCAGGCTAT CTGTTGGACC 
0121 TGCTGCCCAC AGAGATGCCG GAAGAGCCCG AAGCGTGGAA GGATGTCCTC GGCGACATTA 
0181 GTCGCGTCAT CAAGCCGGGA CTGACCCACT GGCAGTCGCC TCACATGCAT GCCTACTACC 
0241 CCACCAGCAC CTCGTATCCC TCCATTGTGG GCGAGATGCT GGCCAGCGGG TTCGGCGTCA 
0301 TCGGATTCAG CTGGATCTGC AGTCCCGCCT GCACAGAACT GGAGGTGGTG GTCATGGACT 
0361 GGCTGGCCAA GTTCCTGAAG CTGCCCGCAC ACTTCCAGCA CGCCAGCGAT GGACCAGGAG 
0421 GCGGGGTGAT CCAGGGATCA GCTAGCGAGG CTGTGTTGGT GGCTGTCCTA GCTGCCAGGG 
0481 AACAA-GCTG TGGCCAACTA C

Assemble Data


10501R-1.IR_full       1   TGGATGCCAAGGAGTTTCGGGAATTCGGCAAGGCCGCCATTGACTACATAGCCGACTATC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10501R-1.in silico     1   TGGATGCCAAGGAGTTTCGGGAATTCGGCAAGGCCGCCATTGACTACATAGCCGACTATC 60


10501R-1.IR_full       61  TGGAGAATATTCGGGATGACGACGTACTGCCCAATGTGGAGCCAGGCTATCTGTTGGACC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10501R-1.in silico     61  TGGAGAATATTCGGGATGACGACGTACTGCCCAATGTGGAGCCAGGCTATCTGTTGGACC 120


10501R-1.IR_full       121 TGCTGCCCACAGAGATGCCGGAAGAGCCCGAAGCGTGGAAGGATGTCCTCGGCGACATTA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10501R-1.in silico     121 TGCTGCCCACAGAGATGCCGGAAGAGCCCGAAGCGTGGAAGGATGTCCTCGGCGACATTA 180


10501R-1.IR_full       181 GTCGCGTCATCAAGCCGGGACTGACCCACTGGCAGTCGCCTCACATGCATGCCTACTACC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10501R-1.in silico     181 GTCGCGTCATCAAGCCGGGACTGACCCACTGGCAGTCGCCTCACATGCATGCCTACTACC 240


10501R-1.IR_full       241 CCACCAGCACCTCGTATCCCTCCATTGTGGGCGAGATGCTGGCCAGCGGGTTCGGCGTCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10501R-1.in silico     241 CCACCAGCACCTCGTATCCCTCCATTGTGGGCGAGATGCTGGCCAGCGGGTTCGGCGTCA 300


10501R-1.IR_full       301 TCGGATTCAGCTGGATCTGCAGTCCCGCCTGCACAGAACTGGAGGTGGTGGTCATGGACT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10501R-1.in silico     301 TCGGATTCAGCTGGATCTGCAGTCCCGCCTGCACAGAACTGGAGGTGGTGGTCATGGACT 360


10501R-1.IR_full       361 GGCTGGCCAAGTTCCTGAAGCTGCCCGCACACTTCCAGCACGCCAGCGATGGACCAGGAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10501R-1.in silico     361 GGCTGGCCAAGTTCCTGAAGCTGCCCGCACACTTCCAGCACGCCAGCGATGGACCAGGAG 420


10501R-1.IR_full       421 GCGGGGTGATCCAGGGATCAGCTAGCGAGGCTGTGTTGGTGGCTGTCCTAGCTGCCAGGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10501R-1.in silico     421 GCGGGGTGATCCAGGGATCAGCTAGCGAGGCTGTGTTGGTGGCTGTCCTAGCTGCCAGGG 480


10501R-1.IR_full       481 AACAATGCTGTGGCCAACTAC 501
                           ||||| |||||||||||||||
10501R-1.in silico     481 AACAA-GCTGTGGCCAACTAC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_057244.3	CG10501-RA, transcript variant A (amd), mRNA
35.47	171	14	8	14	NM_165278.1	CG10501-RB, transcript variant B (amd), mRNA
0.2	1	3	2	3	NM_140314.2	CG10698-RA (GRHRII), mRNA
0	0	5	13	37	NM_165280.1	CG10697-RB, transcript variant B (Ddc), mRNA
0	0	5	13	37	NM_165279.1	CG10697-RC, transcript variant C (Ddc), mRNA
0	0	5	13	37	NM_078876.4	CG10697-RA, transcript variant A (Ddc), mRNA
0	0	2	3	2	NM_132203.2	CG15333-RA (CG15333), mRNA
0	0	2	2	5	NM_134993.1	CG15440-RA (CG15440), mRNA
0	0	1	6	2	NM_057337.2	CG3978-RA, transcript variant A (pnr), mRNA
0	0	1	5	3	NM_143259.1	CG5521-RA (CG5521), mRNA
0	0	1	4	5	NM_080317.2	CG2647-RA (per), mRNA
0	0	1	3	5	NM_142882.1	CG17083-RA (CG17083), mRNA
0	0	1	3	4	NM_143613.2	CG12063-RA (CG12063), mRNA
0	0	1	3	4	NM_165812.1	CG30029-RA (CG30029), mRNA
0	0	1	3	2	NM_134900.2	CG17260-RA (CG17260), mRNA
0	0	1	2	6	NM_139648.1	CG15022-RA (CG15022), mRNA
0	0	1	2	5	NM_164568.1	CG31773-RA (CG31773), mRNA
0	0	1	2	5	NM_079221.2	CG7018-RB, transcript variant B (Ets65A), mRNA
0	0	1	2	5	NM_079985.2	CG5206-RA (bon), mRNA
0	0	1	2	5	NM_137133.2	CG10143-RA (Adgf-E), mRNA
0	0	1	2	4	NM_001043114.1	CG33484-RD, transcript variant D (zormin), mRNA
0	0	1	2	3	NM_167739.2	CG1801-RA (CG1801), mRNA
0	0	1	2	3	NM_167127.1	CG32726-RA (CG32726), mRNA
0	0	1	2	3	NM_130555.1	CG14772-RA (CG14772), mRNA
0	0	1	2	3	NM_079609.2	CG6019-RA (mus308), mRNA
0	0	0	13	32	NM_078955.2	CG3454-RA (Hdc), mRNA
0	0	0	6	9	NM_165026.1	CG16975-RA, transcript variant A (CG16975), mRNA
0	0	0	6	9	NM_135762.2	CG16975-RB, transcript variant B (CG16975), mRNA
0	0	0	5	4	NM_132977.1	CG12997-RA (CG12997), mRNA
0	0	0	5	4	NM_176068.2	CG15218-RA, transcript variant A (CycK), mRNA

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