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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4974R-1 |
| Symbol |
dally |
Full Name |
division abnormally delayed |
| CG No |
CG4974 |
Old CG No |
CG4974 |
| Synonyms |
CG4974, CT15952, gem, Dally, clone 1.63, l(3)06464, anon-EST:Liang-1.63, dally |
| Accession No (Link to NCBI) |
|
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tseng CY, Su YH, Yang SM, Lin KY, Lai CM, Rastegari E, Amartuvshin O, Cho Y, Cai Y, Hsu HJ.
Smad-Independent BMP Signaling in Somatic Cells Limits the Size of the Germline Stem Cell Pool.
Stem Cell Reports (2018) 11(3) 811-827
[
PubMed ID = 30122445
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGACCTGGATGGTATCCACCAC |
| Primer Seq. 3' |
aatctagaggtaccCCCAGTTGGCCATTGCTGTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
|
| in silico PCR Fragment |
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| Assemble Data |
4974R-1.IR full 1 ---------------------------------------------------------AGA 60
|||
4974R-1.in silico 1 GACCTGGATGGTATCCACCACCACCAGCACCACCTGCACAGCGCCACCACGCACCACAGA 60
4974R-1.IR full 61 CGTCGCCTACAGCGGGACTCGCGGNCGAAGGACGC-GTTGGGGGATCGACCCACCAGTGT 120
|||||||||||||||||||||||| |||||||||| ||||||||||||||||||||||||
4974R-1.in silico 61 CGTCGCCTACAGCGGGACTCGCGGGCGAAGGACGCCGTTGGGGGATCGACCCACCAGTGT 120
4974R-1.IR full 121 GATGCCGTCAAGAGCTACTTCGAATCGATCGACATCAAGTCGAGTGGGACTTACAGCGAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-1.in silico 121 GATGCCGTCAAGAGCTACTTCGAATCGATCGACATCAAGTCGAGTGGGACTTACAGCGAA 180
4974R-1.IR full 181 AAAGGCGCCATTTGTGGCGGAAACTGCTGCAACAATGCCACGGAGCTGGAGCTGCGGGAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-1.in silico 181 AAAGGCGCCATTTGTGGCGGAAACTGCTGCAACAATGCCACGGAGCTGGAGCTGCGGGAC 240
4974R-1.IR full 241 AAAGCCGCCGGAATGTTCGAGCAACTGCTTCATCATCACACCAGCAGCCTGCGCGGAGTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-1.in silico 241 AAAGCCGCCGGAATGTTCGAGCAACTGCTTCATCATCACACCAGCAGCCTGCGCGGAGTC 300
4974R-1.IR full 301 CTCGAGACGAATGCCAAACAGTTCCAAAGTCACGTCCTGGAATTGGCGCAGATTTCGGAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-1.in silico 301 CTCGAGACGAATGCCAAACAGTTCCAAAGTCACGTCCTGGAATTGGCGCAGATTTCGGAG 360
4974R-1.IR full 361 AACATGACCCACTCGCTCTTTTCGAAGGTTTATACCCGTATGGTGCCTTCATCGCGTATG 420
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4974R-1.in silico 361 AACATGACCCACTCGCTCTTTTCGAAGGTTTATACCCGTATGGTGCCTTCATCGCGTATG 420
4974R-1.IR full 421 ATGATCCATCAACTGTACACCGAAATCATGAACCATCTGATTTACACCTCCAACTATACG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4974R-1.in silico 421 ATGATCCATCAACTGTACACCGAAATCATGAACCATCTGATTTACACCTCCAACTATACG 480
4974R-1.IR full 481 AACAGCAATGGCCAACTGGG 500
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4974R-1.in silico 481 AACAGCAATGGCCAACTGGG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079259.2 |
division abnormally delayed CG4974-RA (dally), mRNA |
| 0.82 |
4 |
5 |
2 |
6 |
NM_079321.2 |
araucan CG10571-RA (ara), mRNA |
| 0.82 |
4 |
4 |
6 |
19 |
NM_143061.1 |
tenectin CG13648-RA (tnc), mRNA |
| 0.62 |
3 |
12 |
29 |
52 |
NM_143342.1 |
CG5514-RB, transcript variant B (CG5514), mRNA |
| 0.62 |
3 |
12 |
29 |
52 |
NM_170361.1 |
CG5514-RA, transcript variant A (CG5514), mRNA |
| 0.41 |
2 |
2 |
17 |
18 |
NM_057805.2 |
toucan CG9660-RA, transcript variant A (toc), mRNA |
| 0.41 |
2 |
2 |
14 |
15 |
NM_176229.1 |
CG33147-RA (CG33147), mRNA |
| 0.41 |
2 |
2 |
5 |
12 |
NM_135286.1 |
CG6739-RA (CG6739), mRNA |
| 0.2 |
1 |
5 |
13 |
21 |
NM_080362.2 |
SAM-motif ubiquitously expressed punctatedly localized protein CG31868-RA (Samuel), mRNA |
| 0.2 |
1 |
4 |
8 |
20 |
NM_132370.1 |
CG2989-RA (CG2989), mRNA |
| 0.2 |
1 |
3 |
6 |
48 |
NM_134527.1 |
CG15322-RA (CG15322), mRNA |
| 0.2 |
1 |
2 |
9 |
30 |
NM_132004.2 |
CG4136-RA (CG4136), mRNA |
| 0.2 |
1 |
2 |
5 |
13 |
NM_132498.1 |
CG1572-RA, transcript variant A (CG1572), mRNA |
| 0.2 |
1 |
2 |
5 |
13 |
NM_167289.1 |
CG1572-RB, transcript variant B (CG1572), mRNA |
| 0.2 |
1 |
2 |
5 |
10 |
NM_057980.3 |
Accessory gland-specific peptide 32CD CG4605-RA (Acp32CD), mRNA |
| 0.2 |
1 |
2 |
5 |
9 |
NM_167023.1 |
CG32770-RA (CG32770), mRNA |
| 0 |
0 |
10 |
12 |
37 |
NM_132391.2 |
CG15307-RA (CG15307), mRNA |
| 0 |
0 |
9 |
29 |
38 |
NM_140417.2 |
CG17364-RA, transcript variant A (CG17364), mRNA |
| 0 |
0 |
9 |
29 |
38 |
NM_176328.1 |
CG17364-RB, transcript variant B (CG17364), mRNA |
| 0 |
0 |
9 |
19 |
47 |
NM_137672.1 |
CG15225-RA (CG15225), mRNA |
| 0 |
0 |
9 |
15 |
33 |
NM_132214.1 |
CG15335-RB (CG15335), mRNA |
| 0 |
0 |
9 |
9 |
28 |
NM_143571.2 |
CG15544-RA (CG15544), mRNA |
| 0 |
0 |
8 |
9 |
24 |
NM_078544.2 |
lozenge CG1689-RA (lz), mRNA |
| 0 |
0 |
7 |
14 |
47 |
NM_134552.3 |
RhoGAP19D CG1412-RA (RhoGAP19D), mRNA |
| 0 |
0 |
7 |
13 |
34 |
NM_169047.1 |
l(3)82Fd CG32464-RD, transcript variant D (l(3)82Fd), mRNA |
| 0 |
0 |
7 |
11 |
29 |
NM_132009.2 |
CG11473-RA (CG11473), mRNA |
| 0 |
0 |
7 |
9 |
27 |
NM_206663.1 |
CG12075-RB, transcript variant B (CG12075), mRNA |
| 0 |
0 |
7 |
9 |
27 |
NM_132278.2 |
CG12075-RA, transcript variant A (CG12075), mRNA |
| 0 |
0 |
7 |
9 |
21 |
NM_143633.1 |
CG2187-RA (CG2187), mRNA |
| 0 |
0 |
7 |
8 |
33 |
NM_080120.2 |
male-specific opa containing gene CG14560-RA (msopa), mRNA |
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