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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6620R-3 |
| Symbol |
ial |
Full Name |
IplI-aurora-like kinase |
| CG No |
CG6620 |
Old CG No |
CG6620 |
| Synonyms |
aurora B, AurB, IAL1, DmAurB, DmAuroraB, aurB, CG6620, IAL, DmAIRK2, ial, Ial |
| Accession No (Link to NCBI) |
NM_057988.3 |
| Inserted Chr. |
X |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Le Bras S, Rondanino C, Kriegel-Taki G, Dussert A, Le Borgne R.
Genetic identification of intracellular trafficking regulators involved in Notch-dependent binary cell fate acquisition following asymmetric cell division.
J Cell Sci (2012) 125(Pt 20) 4886-901
[
PubMed ID = 22825875
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGACGCTTTCCCGCGCGAAG |
| Primer Seq. 3' |
aatctagaggtaccTTGAGGGCATTCGCCACTTGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGACGCTTT CCCGCGCGAA GcaCGCCAAC CGCAACCACC TGCCGCACCT GCTGGCCAAG
0061 GTGCCCGAGG AGCACCAGGA GCCGATCAAG AATATGTGCC TCAAGATGAT GTCCCACGAC
0121 GCATACGGAC AGCCATACGA TTGGAGTCCC CGGGACTTTG AGATGGGCGC CCACTTGGGA
0181 CGCGGCAAAT TTGGACGTGT CTANCTTGGC GCNGGGAGCG CCACTCGCCA CTATCTGGTG
0241 GCCATGAAGG TGATGTTCAA AGAGGAGCTG CGCAAGGGCT GCGTGCAGCG TCAGGTTCTA
0301 CGCGAGATCG AGATCCAGTC GCGACTGAAG CACCCGCACA TCCTGCGCCT GCTCACTTGG
0361 TTCCACGACG AGAGCCGCAT ATATCTGGCC CTGGAAATTG CGTCCGAGGG CGAGCTGTTC
0421 AAGCACCTGC GTGGCGCACC CAATCATCGA TTCGATGAAC CGCGGTCTGC GAAGTACACC
0481 TACCAAGTgG CGAATGCCCT CAA |
| in silico PCR Fragment |
0001 ATGACGCTTT CCCGCGCGAA GCACGCCAAC CGCAACCACC TGCCGCACCT GCTGGCCAAG
0061 GTGCCCGAGG AGCACCAGGA GCCGATCAAG AATATGTGCC TCAAGATGAT GTCCCACGAC
0121 GCATACGGAC AGCCATACGA TTGGAGTCCC CGGGACTTTG AGATGGGCGC CCACTTGGGA
0181 CGCGGCAAAT TTGGACGTGT CTA-CTTGGC GC-GGGAGCG CCACTCG-CA CTATCTGGTG
0241 GCCATGAAGG TGATGTTCAA AGAGGAGCTG CGCAAGGGCT GCGTGCAGCG TCAGGTTCTA
0301 CGCGAGATCG AGATCCAGTC GCGACTGAAG CACCCGCACA TCCTGCGCCT GCTCACTTGG
0361 TTCCACGACG AGAGCCGCAT CTATCTGGCC CTGGAAATTG CGTCCGAGGG CGAGCTGTTC
0421 AAGCACCTGC GTGGCGCACC CAATCATCGA TTCGATGAAC CGCGGTCTGC GAAGTACACC
0481 TACCAAGTGG CGAATGCCCT CAA |
| Assemble Data |
6620R-3.IR_full 1 ATGACGCTTTCCCGCGCGAAGCACGCCAACCGCAACCACCTGCCGCACCTGCTGGCCAAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6620R-3.in silico 1 ATGACGCTTTCCCGCGCGAAGCACGCCAACCGCAACCACCTGCCGCACCTGCTGGCCAAG 60
6620R-3.IR_full 61 GTGCCCGAGGAGCACCAGGAGCCGATCAAGAATATGTGCCTCAAGATGATGTCCCACGAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6620R-3.in silico 61 GTGCCCGAGGAGCACCAGGAGCCGATCAAGAATATGTGCCTCAAGATGATGTCCCACGAC 120
6620R-3.IR_full 121 GCATACGGACAGCCATACGATTGGAGTCCCCGGGACTTTGAGATGGGCGCCCACTTGGGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6620R-3.in silico 121 GCATACGGACAGCCATACGATTGGAGTCCCCGGGACTTTGAGATGGGCGCCCACTTGGGA 180
6620R-3.IR_full 181 CGCGGCAAATTTGGACGTGTCTANCTTGGCGCNGGGAGCGCCACTCGCCACTATCTGGTG 240
||||||||||||||||||||||| |||||||| |||||||||||||| ||||||||||||
6620R-3.in silico 181 CGCGGCAAATTTGGACGTGTCTA-CTTGGCGC-GGGAGCGCCACTCG-CACTATCTGGTG 240
6620R-3.IR_full 241 GCCATGAAGGTGATGTTCAAAGAGGAGCTGCGCAAGGGCTGCGTGCAGCGTCAGGTTCTA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6620R-3.in silico 241 GCCATGAAGGTGATGTTCAAAGAGGAGCTGCGCAAGGGCTGCGTGCAGCGTCAGGTTCTA 300
6620R-3.IR_full 301 CGCGAGATCGAGATCCAGTCGCGACTGAAGCACCCGCACATCCTGCGCCTGCTCACTTGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6620R-3.in silico 301 CGCGAGATCGAGATCCAGTCGCGACTGAAGCACCCGCACATCCTGCGCCTGCTCACTTGG 360
6620R-3.IR_full 361 TTCCACGACGAGAGCCGCATATATCTGGCCCTGGAAATTGCGTCCGAGGGCGAGCTGTTC 420
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
6620R-3.in silico 361 TTCCACGACGAGAGCCGCATCTATCTGGCCCTGGAAATTGCGTCCGAGGGCGAGCTGTTC 420
6620R-3.IR_full 421 AAGCACCTGCGTGGCGCACCCAATCATCGATTCGATGAACCGCGGTCTGCGAAGTACACC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6620R-3.in silico 421 AAGCACCTGCGTGGCGCACCCAATCATCGATTCGATGAACCGCGGTCTGCGAAGTACACC 480
6620R-3.IR_full 481 TACCAAGTGGCGAATGCCCTCAA 503
|||||||||||||||||||||||
6620R-3.in silico 481 TACCAAGTGGCGAATGCCCTCAA 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057988.3 |
CG6620-RA (ial), mRNA |
| 0.82 |
4 |
2 |
2 |
5 |
NM_132479.2 |
CG1597-RA (CG1597), mRNA |
| 0 |
0 |
6 |
2 |
9 |
NM_142495.1 |
CG14299-RA, transcript variant A (CG14299), mRNA |
| 0 |
0 |
6 |
2 |
8 |
NM_001014640.1 |
CG14299-RB, transcript variant B (CG14299), mRNA |
| 0 |
0 |
2 |
6 |
6 |
NM_078922.2 |
CG4486-RA (Cyp9b2), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_166420.1 |
CG30293-RA (CG30293), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_138005.1 |
CG13562-RA (CG13562), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_137633.1 |
CG16739-RA (CG16739), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_167072.1 |
CG3861-RB, transcript variant B (l(1)G0030), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_132091.2 |
CG3861-RA, transcript variant A (l(1)G0030), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_078520.2 |
CG2212-RA, transcript variant A (sws), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_001014545.1 |
CG33519-RB (Unc-89), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_142112.2 |
CG8279-RA (Pde6), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_169371.1 |
CG6584-RD, transcript variant D (SelR), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_206473.1 |
CG6584-RF, transcript variant F (SelR), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_141773.2 |
CG6584-RA, transcript variant A (SelR), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_169370.2 |
CG6584-RB, transcript variant B (SelR), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_169369.1 |
CG6584-RE, transcript variant E (SelR), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_169368.1 |
CG6584-RC, transcript variant C (SelR), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_140297.2 |
CG9760-RB (CG9760), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_079710.2 |
CG6570-RA (lbl), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_139802.2 |
CG32392-RB, transcript variant B (CG32392), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_168180.1 |
CG32392-RA, transcript variant A (CG32392), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168468.1 |
CG32091-RB (CG32091), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_140760.2 |
CG7430-RA (CG7430), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_134984.2 |
CG3652-RA (CG3652), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_078722.2 |
CG17941-RA (ds), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_057545.3 |
CG14513-RA (yemalpha), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_139615.2 |
CG15006-RA (CG15006), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_078605.1 |
CG6211-RA (gce), mRNA |
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