|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3803R-1 |
| Symbol |
CG3803 |
Full Name |
CG3803 |
| CG No |
CG3803 |
Old CG No |
CG3803 |
| Synonyms |
CG3803 |
| Accession No (Link to NCBI) |
NM_138011.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCATCAGCACTCCGAAAAGCGA |
| Primer Seq. 3' |
aatctagaggtaccGGGAGGCCAATCTATACTGGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CATCAGCACT CCGAAAAGCG ACAAGACCGT CGGAAGATGG TTCCTGGGAG TCAGCGGCAT
0061 GGTCTTCGTG GCCGTTGGTC TAGGCGGAGT AACCCGACTG ACAGAGTCCG GACTGTCCAT
0121 GGTCACTTGG AAGCTGACGG GTGAACGTAT GCCCCGAACC CAGGAGGAAT GGGTGCAGGA
0181 GTTCAATCTC TACCAGCAAT ACCCAGAGTA CAAGCTAAAG AATGTGAATA TGACCGTGGA
0241 GGAGTTCAAG TTCATTTTCA TGATGGAGTA CATGCACAGG ATGTGGGGTC GTGCCATCGG
0301 CGGGTTCTTC CTTTTGCCCG CTGTTTACTT CTGGAGAAAG GGATACTTCT GTGCGAAGAC
0361 CAAGAAGAGC GTCCTGCTGC TGGGCACACT GATTGGCCTG CAAGGTCTGA TGGGATGGTA
0421 CATGGTCAAG TCCGGGCTGG AAGACAGGTT CCAGGATCCC AACGACGTGC CACGGGTATC
0481 CCAGTATAGA TTGGcCTCCC |
| in silico PCR Fragment |
0001 CATCAGCACT CCGAAAAGCG ACAAGACCGT CGGAAGATGG TTCCTGGGAG TCAGCGGCAT
0061 GGTCTTCGTG GCCGTTGGTC TAGGCGGAGT AACCCGACTG ACAGAGTCCG GACTGTCCAT
0121 GGTCACTTGG AAGCTGACGG GTGAACGTAT GCCCCGAACC CAGGAGGAAT GGGTGCAGGA
0181 GTTCAATCTC TACCAGCAAT ACCCAGAGTA CAAGCTAAAG AATGTGAATA TGACCGTGGA
0241 GGAGTTCAAG TTCATTTTCA TGATGGAGTA CATGCACAGG ATGTGGGGTC GTGCCATCGG
0301 CGGGTTCTTC CTTTTGCCCG CTGTTTACTT CTGGAGAAAG GGATACTTCT GTGCGAAGAC
0361 CAAGAAGAGC GTCCTGCTGC TGGGCACACT GATTGGCCTG CAAGGTCTGA TGGGATGGTA
0421 CATGGTCAAG TCCGGGCTGG AAGACAGGTT CCAGGATCCC AACGACGTGC CACGGGTATC
0481 CCAGTATAGA TTGGCCTCCC |
| Assemble Data |
3803R-1.IR_full 1 CATCAGCACTCCGAAAAGCGACAAGACCGTCGGAAGATGGTTCCTGGGAGTCAGCGGCAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico 1 CATCAGCACTCCGAAAAGCGACAAGACCGTCGGAAGATGGTTCCTGGGAGTCAGCGGCAT 60
3803R-1.IR_full 61 GGTCTTCGTGGCCGTTGGTCTAGGCGGAGTAACCCGACTGACAGAGTCCGGACTGTCCAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico 61 GGTCTTCGTGGCCGTTGGTCTAGGCGGAGTAACCCGACTGACAGAGTCCGGACTGTCCAT 120
3803R-1.IR_full 121 GGTCACTTGGAAGCTGACGGGTGAACGTATGCCCCGAACCCAGGAGGAATGGGTGCAGGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico 121 GGTCACTTGGAAGCTGACGGGTGAACGTATGCCCCGAACCCAGGAGGAATGGGTGCAGGA 180
3803R-1.IR_full 181 GTTCAATCTCTACCAGCAATACCCAGAGTACAAGCTAAAGAATGTGAATATGACCGTGGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico 181 GTTCAATCTCTACCAGCAATACCCAGAGTACAAGCTAAAGAATGTGAATATGACCGTGGA 240
3803R-1.IR_full 241 GGAGTTCAAGTTCATTTTCATGATGGAGTACATGCACAGGATGTGGGGTCGTGCCATCGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico 241 GGAGTTCAAGTTCATTTTCATGATGGAGTACATGCACAGGATGTGGGGTCGTGCCATCGG 300
3803R-1.IR_full 301 CGGGTTCTTCCTTTTGCCCGCTGTTTACTTCTGGAGAAAGGGATACTTCTGTGCGAAGAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico 301 CGGGTTCTTCCTTTTGCCCGCTGTTTACTTCTGGAGAAAGGGATACTTCTGTGCGAAGAC 360
3803R-1.IR_full 361 CAAGAAGAGCGTCCTGCTGCTGGGCACACTGATTGGCCTGCAAGGTCTGATGGGATGGTA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico 361 CAAGAAGAGCGTCCTGCTGCTGGGCACACTGATTGGCCTGCAAGGTCTGATGGGATGGTA 420
3803R-1.IR_full 421 CATGGTCAAGTCCGGGCTGGAAGACAGGTTCCAGGATCCCAACGACGTGCCACGGGTATC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico 421 CATGGTCAAGTCCGGGCTGGAAGACAGGTTCCAGGATCCCAACGACGTGCCACGGGTATC 480
3803R-1.IR_full 481 CCAGTATAGATTGGCCTCCC 500
||||||||||||||||||||
3803R-1.in silico 481 CCAGTATAGATTGGCCTCCC 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_138011.2 |
CG3803-RA (CG3803), mRNA |
| 0.2 |
1 |
2 |
3 |
7 |
NM_136875.2 |
CG17509-RA (CG17509), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_206278.1 |
CG5406-RC, transcript variant C (sif), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_079908.2 |
CG5406-RA, transcript variant A (sif), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_057983.2 |
CG8823-RA (Lip3), mRNA |
| 0 |
0 |
1 |
4 |
9 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_001032052.1 |
CG33715-RE, transcript variant E (Msp-300), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_176579.1 |
CG33203-RC (CG33203), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_080102.3 |
CG10506-RA (Aats-gln), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_143665.2 |
CG2041-RA (lgs), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_176570.1 |
CG33083-RA (Gr97a), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_176694.1 |
CG3126-RB, transcript variant B (C3G), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132122.2 |
CG3126-RA, transcript variant A (C3G), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_134961.1 |
CG10019-RA (CG10019), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_078690.2 |
CG12530-RA, transcript variant A (Cdc42), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_167677.1 |
CG12530-RB, transcript variant B (Cdc42), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_001038969.1 |
CG12753-RB, transcript variant B (CG12753), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_142285.1 |
CG12753-RA, transcript variant A (CG12753), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_134281.1 |
CG8651-RC, transcript variant C (trx), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_057421.2 |
CG8651-RD, transcript variant D (trx), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_057422.2 |
CG8651-RB, transcript variant B (trx), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001014621.1 |
CG8651-RE, transcript variant E (trx), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_134282.1 |
CG8651-RA, transcript variant A (trx), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_142784.2 |
CG13852-RA (mats), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_137971.2 |
CG5428-RA (CG5428), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_142267.2 |
CG5916-RA (CG5916), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_133148.2 |
CG8034-RA (CG8034), mRNA |
| 0 |
0 |
0 |
3 |
12 |
NM_078670.2 |
CG7092-RA (Dhc16F), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_168171.1 |
CG32397-RA (CG32397), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| ++ |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
|
|
|
|
