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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1063R-2 |
| Symbol |
Itp-r83A |
Full Name |
Inositol 1,4,5,-tris-phosphate receptor |
| CG No |
CG1063 |
Old CG No |
CG1063 |
| Synonyms |
itpr, IP3R, IP[[3]]R, DmInsP[[3]]R, DmInsP[[3R]], InsP[[3]]R, dip, IP[[3]] receptor, CG1063, Itp-r[1], InsP[[3]], unnamed, l(3)05616, DIP, l(3)j5B4, Itp-r42B, Itp-r, Insp3R, IP, anon-WO02059370.49, Itp-r83A |
| Accession No (Link to NCBI) |
NM_169061.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
semi-lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Agrawal N, Lawler K, Davidson CM, Keogh JM, Legg R, INTERVAL, Barroso I, Farooqi IS, Brand AH.
Predicting novel candidate human obesity genes and their site of action by systematic functional screening in Drosophila.
PLoS Biol (2021) 19(11) e3001255
[
PubMed ID = 34748544
]
[
RRC reference
]
Ravi P, Trivedi D, Hasan G.
FMRFa receptor stimulated Ca2+ signals alter the activity of flight modulating central dopaminergic neurons in Drosophila melanogaster.
PLoS Genet (2018) 14(8) e1007459
[
PubMed ID = 30110323
]
[
RRC reference
]
Deb BK, Pathak T, Hasan G.
Store-independent modulation of Ca(2+) entry through Orai by Septin 7.
Nat Commun (2016) 7 11751
[
PubMed ID = 27225060
]
[
RRC reference
]
Chakraborty S, Hasan G.
Spontaneous Ca2+ Influx in Drosophila Pupal Neurons Is Modulated by IP3-Receptor Function and Influences Maturation of the Flight Circuit.
Front Mol Neurosci (2017) 10 111
[
PubMed ID = 28473752
]
[
RRC reference
]
Richhariya S, Jayakumar S, Abruzzi K, Rosbash M, Hasan G.
A pupal transcriptomic screen identifies Ral as a target of store-operated calcium entry in Drosophila neurons.
Sci Rep (2017) 7 42586
[
PubMed ID = 28195208
]
[
RRC reference
]
Chakraborty S, Deb BK, Chorna T, Konieczny V, Taylor CW, Hasan G.
Mutant IP3 receptors attenuate store-operated Ca2+ entry by destabilizing STIM-Orai interactions in Drosophila neurons.
J Cell Sci (2016) 129(20) 3903-3910
[
PubMed ID = 27591258
]
[
RRC reference
]
Jayakumar S, Richhariya S, Reddy OV, Texada MJ, Hasan G.
Drosophila larval to pupal switch under nutrient stress requires IP3R/Ca(2+) signalling in glutamatergic interneurons.
Elife (2016) 5
[
PubMed ID = 27494275
]
[
RRC reference
]
Restrepo S, Basler K.
Drosophila wing imaginal discs respond to mechanical injury via slow InsP3R-mediated intercellular calcium waves.
Nat Commun (2016) 7 12450
[
PubMed ID = 27503836
]
[
RRC reference
]
Agrawal T, Hasan G.
Maturation of a central brain flight circuit in Drosophila requires Fz2/Ca²⁺ signaling.
Elife (2015) 4
[
PubMed ID = 25955970
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Pathak T, Agrawal T, Richhariya S, Sadaf S, Hasan G.
Store-Operated Calcium Entry through Orai Is Required for Transcriptional Maturation of the Flight Circuit in Drosophila.
J Neurosci (2015) 35(40) 13784-99
[
PubMed ID = 26446229
]
[
RRC reference
]
Subramanian M, Jayakumar S, Richhariya S, Hasan G.
Loss of IP3 receptor function in neuropeptide secreting neurons leads to obesity in adult Drosophila.
BMC Neurosci (2013) 14 157
[
PubMed ID = 24350669
]
[
RRC reference
]
Agrawal T, Sadaf S, Hasan G.
A genetic RNAi screen for IP₃/Ca²⁺ coupled GPCRs in Drosophila identifies the PdfR as a regulator of insect flight.
PLoS Genet (2013) 9(10) e1003849
[
PubMed ID = 24098151
]
[
RRC reference
]
Murmu MS, Stinnakre J, Martin JR.
Presynaptic Ca2+ stores contribute to odor-induced responses in Drosophila olfactory receptor neurons.
J Exp Biol (2010) 213(Pt 24) 4163-73
[
PubMed ID = 21112997
]
[
RRC reference
]
Sadaf S, Birman S, Hasan G.
Synaptic activity in serotonergic neurons is required for air-puff stimulated flight in Drosophila melanogaster.
PLoS One (2012) 7(9) e46405
[
PubMed ID = 23029511
]
[
RRC reference
]
Kain P, Badsha F, Hussain SM, Nair A, Hasan G, Rodrigues V.
Mutants in phospholipid signaling attenuate the behavioral response of adult Drosophila to trehalose.
Chem Senses (2010) 35(8) 663-73
[
PubMed ID = 20543015
]
[
RRC reference
]
Agrawal N, Venkiteswaran G, Sadaf S, Padmanabhan N, Banerjee S, Hasan G.
Inositol 1,4,5-trisphosphate receptor and dSTIM function in Drosophila insulin-producing neurons regulates systemic intracellular calcium homeostasis and flight.
J Neurosci (2010) 30(4) 1301-13
[
PubMed ID = 20107057
]
[
RRC reference
]
Subramanian M, Metya SK, Sadaf S, Kumar S, Schwudke D, Hasan G.
Altered lipid homeostasis in Drosophila InsP3 receptor mutants leads to obesity and hyperphagia.
Dis Model Mech (2013) 6(3) 734-44
[
PubMed ID = 23471909
]
[
RRC reference
]
Murmu MS, Stinnakre J, Réal E, Martin JR.
Calcium-stores mediate adaptation in axon terminals of olfactory receptor neurons in Drosophila.
BMC Neurosci (2011) 12 105
[
PubMed ID = 22024464
]
[
RRC reference
]
Usui-Aoki K, Matsumoto K, Koganezawa M, Kohatsu S, Isono K, Matsubayashi H, Yamamoto MT, Ueda R, Takahashi K, Saigo K, Mikoshiba K, Yamamoto D.
Targeted expression of Ip3 sponge and Ip3 dsRNA impaires sugar taste sensation in Drosophila.
J Neurogenet (2005) 19(3-4) 123-41
[
PubMed ID = 16540404
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAAGCTTCCTGCACTTGGGGG |
| Primer Seq. 3' |
aatctagaggtaccGGAGCGAAGCTTGTAGAAGGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CAAGCTTCCT GCACTTGGGG GACATTGTGT cCCTCTATGC GGAGGGCAGC GTTTGCGNTT
0061 TCTTGAGCAC ACTCGGCCTG GTCGATGATC GCACAGTGGT GTGTCCCGAG GCCGGAGATC
0121 TCAGCTGTCC GCCTAAGAAG TTCAGGGACT GCCTCATCAA AATATGCCCC ATGAACCGCT
0181 ATTCTGCGCA NAAGCAATTT TGGAAAGCAG CTAAGCAATC GGCCAGCTCC AATACGGATC
0241 CCAATCTCCT CAAGCGTTTG CATCATGCGG CGGAGATCGA GAAGAAACAA AACGAGACGG
0301 AGAACAAGAA GCTGCTGGGC ACCGTCAATC CAATATGGAC GAGCTGTGGT GCAGCTACTG
0361 CATNTTGAAG TCCAACAAAT ATTTAACAGT TAACAAGCGA CTGCCGTCGC TGCTGGAGAA
0421 GAATgNCATG CGCGTTTATC TGGATGCCAA CGGNAACGAG GGGTCGTGGT TCTACATCAA
0481 GCCCTTCTAN ANGCTTCGCN CC |
| in silico PCR Fragment |
0001 CAAGCTTCCT GCACTTGGGG GACATTGTGT CCCTCTATGC GGAGGGCAGC GTTTGCGGTT
0061 TCTTGAGCAC ACTCGGCCTG GTCGATGATC GCACAGTGGT GTGTCCCGAG GCCGGAGATC
0121 TCAGCTGTCC GCCTAAGAAG TTCAGGGACT GCCTCATCAA AATATGCCCC ATGAACCGCT
0181 ATTCTGCGCA GAAGCAATTT TGGAAAGCAG CTAAGCAATC GGCCAGCTCC AATACGGATC
0241 CCAATCTCCT CAAGCGTTTG CATCATGCGG CGGAGATCGA GAAGAAACAA AACGAGACGG
0301 AGAACAAGAA GCTGCTGGGC ACC-TCAATC CAATATGGAC GAGCTGTGGT GCAGCTACTG
0361 CAT-TTGAAG TCCAACAAAT ATTTAACAGT TAACAAGCGA CTGCCGTCGC TGCTGGAGAA
0421 GAATGCCATG CGCGTTTATC TGGATGCCAA CGGGAACGAG GGGTCGTGGT TCTACATCAA
0481 GCCCTTCTAC AAGCTTCGCT CC |
| Assemble Data |
1063R-2.IR_full 1 CAAGCTTCCTGCACTTGGGGGACATTGTGTCCCTCTATGCGGAGGGCAGCGTTTGCGNTT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
1063R-2.in silico 1 CAAGCTTCCTGCACTTGGGGGACATTGTGTCCCTCTATGCGGAGGGCAGCGTTTGCGGTT 60
1063R-2.IR_full 61 TCTTGAGCACACTCGGCCTGGTCGATGATCGCACAGTGGTGTGTCCCGAGGCCGGAGATC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1063R-2.in silico 61 TCTTGAGCACACTCGGCCTGGTCGATGATCGCACAGTGGTGTGTCCCGAGGCCGGAGATC 120
1063R-2.IR_full 121 TCAGCTGTCCGCCTAAGAAGTTCAGGGACTGCCTCATCAAAATATGCCCCATGAACCGCT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1063R-2.in silico 121 TCAGCTGTCCGCCTAAGAAGTTCAGGGACTGCCTCATCAAAATATGCCCCATGAACCGCT 180
1063R-2.IR_full 181 ATTCTGCGCANAAGCAATTTTGGAAAGCAGCTAAGCAATCGGCCAGCTCCAATACGGATC 240
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
1063R-2.in silico 181 ATTCTGCGCAGAAGCAATTTTGGAAAGCAGCTAAGCAATCGGCCAGCTCCAATACGGATC 240
1063R-2.IR_full 241 CCAATCTCCTCAAGCGTTTGCATCATGCGGCGGAGATCGAGAAGAAACAAAACGAGACGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1063R-2.in silico 241 CCAATCTCCTCAAGCGTTTGCATCATGCGGCGGAGATCGAGAAGAAACAAAACGAGACGG 300
1063R-2.IR_full 301 AGAACAAGAAGCTGCTGGGCACCGTCAATCCAATATGGACGAGCTGTGGTGCAGCTACTG 360
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
1063R-2.in silico 301 AGAACAAGAAGCTGCTGGGCACC-TCAATCCAATATGGACGAGCTGTGGTGCAGCTACTG 360
1063R-2.IR_full 361 CATNTTGAAGTCCAACAAATATTTAACAGTTAACAAGCGACTGCCGTCGCTGCTGGAGAA 420
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1063R-2.in silico 361 CAT-TTGAAGTCCAACAAATATTTAACAGTTAACAAGCGACTGCCGTCGCTGCTGGAGAA 420
1063R-2.IR_full 421 GAATGNCATGCGCGTTTATCTGGATGCCAACGGNAACGAGGGGTCGTGGTTCTACATCAA 480
||||| ||||||||||||||||||||||||||| ||||||||||||||||||||||||||
1063R-2.in silico 421 GAATGCCATGCGCGTTTATCTGGATGCCAACGGGAACGAGGGGTCGTGGTTCTACATCAA 480
1063R-2.IR_full 481 GCCCTTCTANANGCTTCGCNCC 502
||||||||| | ||||||| ||
1063R-2.in silico 481 GCCCTTCTACAAGCTTCGCTCC 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
1 |
6 |
NM_169061.1 |
CG7896-RA (CG7896), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_169060.1 |
CG7896-RA (CG7896), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA |
| 0.2 |
1 |
4 |
2 |
6 |
NM_169390.2 |
CG31374-RB, transcript variant B (CG31374), mRNA |
| 0.2 |
1 |
4 |
2 |
6 |
NM_176460.1 |
CG31374-RC, transcript variant C (CG31374), mRNA |
| 0.2 |
1 |
4 |
2 |
6 |
NM_169391.1 |
CG31374-RA, transcript variant A (CG31374), mRNA |
| 0.2 |
1 |
3 |
2 |
2 |
NM_141388.2 |
CG1249-RA (CG1249), mRNA |
| 0 |
0 |
5 |
3 |
3 |
NM_142467.2 |
CG7685-RA (CG7685), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_170178.1 |
CG31381-RA (CG31381), mRNA |
| 0 |
0 |
3 |
5 |
5 |
NM_176539.1 |
CG33110-RA (CG33110), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_164707.1 |
CG11199-RB, transcript variant B (Liprin-alpha), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_135223.2 |
CG11199-RA, transcript variant A (Liprin-alpha), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_132740.1 |
CG15890-RA (CG15890), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_132789.2 |
CG6170-RA, transcript variant A (HDAC6), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167437.1 |
CG6170-RC, transcript variant C (HDAC6), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167436.1 |
CG6170-RB, transcript variant B (HDAC6), mRNA |
| 0 |
0 |
2 |
2 |
12 |
NM_142405.1 |
CG18012-RA (CG18012), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_176104.1 |
CG33087-RC (CG33087), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165623.1 |
CG30356-RA (CG30356), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_079067.2 |
CG11949-RA, transcript variant A (cora), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_166338.1 |
CG11949-RD, transcript variant D (cora), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_166336.1 |
CG11949-RC, transcript variant C (cora), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_166337.1 |
CG11949-RB, transcript variant B (cora), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_135483.2 |
CG31712-RA (CG31712), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_139978.3 |
CG6781-RA (CG6781), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_169810.1 |
CG18617-RA, transcript variant A (Vha100-2), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_142465.1 |
CG18617-RB, transcript variant B (Vha100-2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_138985.2 |
CG8947-RA (26-29-p), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168235.1 |
CG17888-RB, transcript variant B (Pdp1), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168236.1 |
CG17888-RG, transcript variant G (Pdp1), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_139458.2 |
CG1317-RB (CG1317), mRNA |
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