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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10014R-2 |
| Symbol |
CG10014 |
Full Name |
CG10014 |
| CG No |
CG10014 |
Old CG No |
CG10014 |
| Synonyms |
CG10014 |
| Accession No (Link to NCBI) |
NM_141907.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
larval lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGATGTGGACATCCTAGAGGAG |
| Primer Seq. 3' |
aatctagaggtaccGGTACTGTCGAGAGATTTACG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GATGTGGACA TCCTAGAGGA GCTGCTGCAG AAGTGCAAGT TTACCGACGA GGAGCTGGTC
0061 ATGTCACGGC CGGCCATCGA GAGTGCCTAC AAGCGCTTCG TGGACGCCTA TATGTCCGCC
0121 GAACGTGGAG CCGATGGAAC GGAGCTACTC TACCAGTACT TCCGGGACAG GGAACTGAAG
0181 CGTATCAACG AGGCCATGCG CAACCAGGCA GCCATCACGA TCCAGGCGGC TTGGCGAGGA
0241 TACTGGGTTC GCCTCCAGTT CCCGCAAGAA GTTTGCGTTT GTGTCTGCGC AGCGGAAAAA
0301 GAGGACGACG GGGAGGAAAA GCGCAGGGAA CAGGCAGCGA GCGTTCTTCA GAGATTCTTC
0361 CGCAAGGTTA TGTTGCGCGT GGTTACCAAG CCAATCGTAG ACCCCTGTGC CGAACCAACG
0421 GAACCAACTG AAGTGGATGC TAGCTCTTCA CCGGATACAG CAAAGGATGG CTATGACGAC
0481 GTAAATCTCT CGACAGTACC |
| in silico PCR Fragment |
0001 GATGTGGACA TCCTAGAGGA GCTGCTGCAG AAGTGCAAGT TTACCGACGA GGAGCTGGTC
0061 ATGTCACGGC CGGCCATCGA GAGTGCCTAC AAGCGCTTCG TGGACGCCTA TATGTCCGCC
0121 GAACGTGGAG CCGATGGAAC GGAGCTACTC TACCAGTACT TCCGGGACAG GGAACTGAAG
0181 CGTATCAACG AGGCCATGCG CAACCAGGCA GCCATCACGA TCCAGGCGGC TTGGCGAGGA
0241 TACTGGGTTC GCCTCCAGTT CCCGCAAGAA GTTTGCGTTT GTGTCTGCGC AGCGGAAAAA
0301 GAGGACGACG GGGAGGAAAA GCGCAGGGAA CAGGCAGCGA GCGTTCTTCA GAGATTCTTC
0361 CGCAAGGTTA TGTTGCGCGT GGTTACCAAG CCAATCGTAG ACCCCTGTGC CGAACCAACG
0421 GAACCAACTG AAGTGGATGC TAGCTCTTCA CCGGATACAG CAAAGGATGG CTATGACGAC
0481 GTAAATCTCT CGACAGTACC |
| Assemble Data |
10014R-2.IR_full 1 GATGTGGACATCCTAGAGGAGCTGCTGCAGAAGTGCAAGTTTACCGACGAGGAGCTGGTC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10014R-2.in silico 1 GATGTGGACATCCTAGAGGAGCTGCTGCAGAAGTGCAAGTTTACCGACGAGGAGCTGGTC 60
10014R-2.IR_full 61 ATGTCACGGCCGGCCATCGAGAGTGCCTACAAGCGCTTCGTGGACGCCTATATGTCCGCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10014R-2.in silico 61 ATGTCACGGCCGGCCATCGAGAGTGCCTACAAGCGCTTCGTGGACGCCTATATGTCCGCC 120
10014R-2.IR_full 121 GAACGTGGAGCCGATGGAACGGAGCTACTCTACCAGTACTTCCGGGACAGGGAACTGAAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10014R-2.in silico 121 GAACGTGGAGCCGATGGAACGGAGCTACTCTACCAGTACTTCCGGGACAGGGAACTGAAG 180
10014R-2.IR_full 181 CGTATCAACGAGGCCATGCGCAACCAGGCAGCCATCACGATCCAGGCGGCTTGGCGAGGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10014R-2.in silico 181 CGTATCAACGAGGCCATGCGCAACCAGGCAGCCATCACGATCCAGGCGGCTTGGCGAGGA 240
10014R-2.IR_full 241 TACTGGGTTCGCCTCCAGTTCCCGCAAGAAGTTTGCGTTTGTGTCTGCGCAGCGGAAAAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10014R-2.in silico 241 TACTGGGTTCGCCTCCAGTTCCCGCAAGAAGTTTGCGTTTGTGTCTGCGCAGCGGAAAAA 300
10014R-2.IR_full 301 GAGGACGACGGGGAGGAAAAGCGCAGGGAACAGGCAGCGAGCGTTCTTCAGAGATTCTTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10014R-2.in silico 301 GAGGACGACGGGGAGGAAAAGCGCAGGGAACAGGCAGCGAGCGTTCTTCAGAGATTCTTC 360
10014R-2.IR_full 361 CGCAAGGTTATGTTGCGCGTGGTTACCAAGCCAATCGTAGACCCCTGTGCCGAACCAACG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10014R-2.in silico 361 CGCAAGGTTATGTTGCGCGTGGTTACCAAGCCAATCGTAGACCCCTGTGCCGAACCAACG 420
10014R-2.IR_full 421 GAACCAACTGAAGTGGATGCTAGCTCTTCACCGGATACAGCAAAGGATGGCTATGACGAC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10014R-2.in silico 421 GAACCAACTGAAGTGGATGCTAGCTCTTCACCGGATACAGCAAAGGATGGCTATGACGAC 480
10014R-2.IR_full 481 GTAAATCTCTCGACAGTACC 500
||||||||||||||||||||
10014R-2.in silico 481 GTAAATCTCTCGACAGTACC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_141907.2 |
CG10014-RA (CG10014), mRNA |
| 0 |
0 |
5 |
2 |
3 |
NM_141825.1 |
CG14708-RA (CG14708), mRNA |
| 0 |
0 |
4 |
3 |
2 |
NM_078870.2 |
CG7157-RA (Acp36DE), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_137555.2 |
CG7097-RB, transcript variant B (CG7097), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_166335.2 |
CG7097-RA, transcript variant A (CG7097), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_165585.1 |
CG12769-RB, transcript variant B (CG12769), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_136503.2 |
CG12769-RA, transcript variant A (CG12769), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_139844.2 |
CG8606-RA, transcript variant A (RhoGEF4), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_168199.1 |
CG8606-RB, transcript variant B (RhoGEF4), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_079861.2 |
CG1455-RA (CanA1), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167847.1 |
CG18214-RC, transcript variant C (trio), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_143703.2 |
CG18214-RA, transcript variant A (trio), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_057516.3 |
CG5403-RA, transcript variant A (retn), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_176254.1 |
CG5403-RB, transcript variant B (retn), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_138001.2 |
CG17658-RA (CG17658), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043313.1 |
CG34155-RA (CG34155), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_168681.2 |
CG3849-RB, transcript variant B (Lasp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_140655.1 |
CG3849-RA, transcript variant A (Lasp), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_057714.3 |
CG12296-RA (klu), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_135705.2 |
CG6618-RB, transcript variant B (Patsas), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_164993.1 |
CG6618-RA, transcript variant A (Patsas), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_135219.2 |
CG11329-RA (CG11329), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166971.1 |
CG32498-RL, transcript variant L (dnc), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_132413.1 |
CG15295-RA (CG15295), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_143692.2 |
CG2999-RA, transcript variant A (unc-13), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_166793.2 |
CG2999-RC, transcript variant C (unc-13), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169968.1 |
CG6706-RA, transcript variant A (GABA-B-R2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079714.2 |
CG6706-RB, transcript variant B (GABA-B-R2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142956.1 |
CG18428-RA (CG18428), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079682.2 |
CG7411-RA (ort), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| +- |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| ++ |
|
|
| Dcompartment |
|
Penetrance |
| ++ |
|
- |
|
|
|
|
