NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10067R-1

Symbol

Act57B

Full Name

Actin 57B

CG No

CG10067

Old CG No

CG10067

Synonyms

Actin, actin, CG10067, T10, C, CG30294, act 57A, M, act57A, Act57A, BEST:RE38067, anon-EST:CL2c12, Act57B

Accession No (Link to NCBI)

NM_079076.3

Inserted Chr.

lll

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Nakaoka T, Iga M, Yamada T, Koujima I, Takeshima M, Zhou X, Suzuki Y, Ogihara MH, Kataoka H.
Deep sequencing of the prothoracic gland transcriptome reveals new players in insect ecdysteroidogenesis.
PLoS One (2017) 12(3) e0172951 [ PubMed ID = 28257485 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGAAGTTGCTGCTCTGGTCGT

Primer Seq. 3'

aatctagaggtaccCATAACCCTCATAGATGGGGA

Predicted Fragment Size

IR fragment full Seq

0001 TGAAGTTGCT GCTCTGGTCG TTGACAATGG CTCCGGCATG TGCAAGGCCg GTTTCGCCGG 
0061 TGATGACGCT CCCCGTGCCG TCTTCCCCTC CATCGTCGGT CGTCCACGCC ACCAGGGTGT 
0121 GATGGTCGGT ATGGGCCAGA AGGACTCGTA CGTCGGTGAT GAGGCCCAGT CCAAGCGTGG 
0181 TATCCTCACC CTGAAATACC CCATCGAGCA CGGTATCATC ACCAACTGGG ATGATATGGA 
0241 GAAGATCTGG CACCACACCT TCTACAACGA GCTGCGTGTT GCCCCCGAGG AGCACCCCGT 
0301 CCTGCTGACT GAGGCCCCCC TGAACCCCAA GGCTAACCGC GAGAAGATGA CCCAGATCAT 
0361 GTTTGAGACC TTCAACTCGC CCGCCATGTA CGTCGCCATC CAGGCCGTGC TCTCCCTGTA 
0421 CGCCTCCGGT CGTACCACCG GTATCGTTCT GGACTCCGGT GATGGTGTCT CCCACACCGT 
0481 CCCCATCTAT GAGGGTTATG

in silico PCR Fragment

0001 TGAAGTTGCT GCTCTGGTCG TTGACAATGG CTCCGGCATG TGCAAGGCCG GTTTCGCCGG 
0061 TGATGACGCT CCCCGTGCCG TCTTCCCCTC CATCGTCGGT CGTCCACGCC ACCAGGGTGT 
0121 GATGGTCGGT ATGGGCCAGA AGGACTCGTA CGTCGGTGAT GAGGCCCAGT CCAAGCGTGG 
0181 TATCCTCACC CTGAAATACC CCATCGAGCA CGGTATCATC ACCAACTGGG ATGATATGGA 
0241 GAAGATCTGG CACCACACCT TCTACAACGA GCTGCGTGTT GCCCCCGAGG AGCACCCCGT 
0301 CCTGCTGACT GAGGCCCCCC TGAACCCCAA GGCTAACCGC GAGAAGATGA CCCAGATCAT 
0361 GTTTGAGACC TTCAACTCGC CCGCCATGTA CGTCGCCATC CAGGCCGTGC TCTCCCTGTA 
0421 CGCCTCCGGT CGTACCACCG GTATCGTTCT GGACTCCGGT GATGGTGTCT CCCACACCGT 
0481 CCCCATCTAT GAGGGTTATG

Assemble Data


10067R-1.IR_full       1   TGAAGTTGCTGCTCTGGTCGTTGACAATGGCTCCGGCATGTGCAAGGCCGGTTTCGCCGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-1.in silico     1   TGAAGTTGCTGCTCTGGTCGTTGACAATGGCTCCGGCATGTGCAAGGCCGGTTTCGCCGG 60


10067R-1.IR_full       61  TGATGACGCTCCCCGTGCCGTCTTCCCCTCCATCGTCGGTCGTCCACGCCACCAGGGTGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-1.in silico     61  TGATGACGCTCCCCGTGCCGTCTTCCCCTCCATCGTCGGTCGTCCACGCCACCAGGGTGT 120


10067R-1.IR_full       121 GATGGTCGGTATGGGCCAGAAGGACTCGTACGTCGGTGATGAGGCCCAGTCCAAGCGTGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-1.in silico     121 GATGGTCGGTATGGGCCAGAAGGACTCGTACGTCGGTGATGAGGCCCAGTCCAAGCGTGG 180


10067R-1.IR_full       181 TATCCTCACCCTGAAATACCCCATCGAGCACGGTATCATCACCAACTGGGATGATATGGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-1.in silico     181 TATCCTCACCCTGAAATACCCCATCGAGCACGGTATCATCACCAACTGGGATGATATGGA 240


10067R-1.IR_full       241 GAAGATCTGGCACCACACCTTCTACAACGAGCTGCGTGTTGCCCCCGAGGAGCACCCCGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-1.in silico     241 GAAGATCTGGCACCACACCTTCTACAACGAGCTGCGTGTTGCCCCCGAGGAGCACCCCGT 300


10067R-1.IR_full       301 CCTGCTGACTGAGGCCCCCCTGAACCCCAAGGCTAACCGCGAGAAGATGACCCAGATCAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-1.in silico     301 CCTGCTGACTGAGGCCCCCCTGAACCCCAAGGCTAACCGCGAGAAGATGACCCAGATCAT 360


10067R-1.IR_full       361 GTTTGAGACCTTCAACTCGCCCGCCATGTACGTCGCCATCCAGGCCGTGCTCTCCCTGTA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-1.in silico     361 GTTTGAGACCTTCAACTCGCCCGCCATGTACGTCGCCATCCAGGCCGTGCTCTCCCTGTA 420


10067R-1.IR_full       421 CGCCTCCGGTCGTACCACCGGTATCGTTCTGGACTCCGGTGATGGTGTCTCCCACACCGT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10067R-1.in silico     421 CGCCTCCGGTCGTACCACCGGTATCGTTCTGGACTCCGGTGATGGTGTCTCCCACACCGT 480


10067R-1.IR_full       481 CCCCATCTATGAGGGTTATG 500
                           ||||||||||||||||||||
10067R-1.in silico     481 CCCCATCTATGAGGGTTATG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_079076.3	CG10067-RA, transcript variant A (Act57B), mRNA
96.68	466	2	3	2	NM_166414.1	CG10067-RB, transcript variant B (Act57B), mRNA
13.07	63	158	154	65	NM_001014726.1	CG4027-RC, transcript variant C (Act5C), mRNA
13.07	63	158	154	65	NM_001014725.1	CG4027-RD, transcript variant D (Act5C), mRNA
13.07	63	158	154	65	NM_167053.1	CG4027-RA, transcript variant A (Act5C), mRNA
13.07	63	158	154	65	NM_078497.2	CG4027-RB, transcript variant B (Act5C), mRNA
11.61	56	126	142	105	NM_169525.1	CG18290-RB, transcript variant B (Act87E), mRNA
11.61	56	126	142	105	NM_057743.3	CG18290-RA, transcript variant A (Act87E), mRNA
11.41	55	148	132	69	NM_079643.1	CG5178-RA (Act88F), mRNA
10.58	51	158	102	95	NM_079486.3	CG7478-RA (Act79B), mRNA
0.2	1	3	4	2	NM_143725.2	CG18039-RA (KaiRIA), mRNA
0	0	40	159	149	NM_078901.2	CG12051-RA (Act42A), mRNA
0	0	3	13	52	NM_057689.1	CG5409-RA (Arp53D), mRNA
0	0	3	3	2	NM_057627.3	CG8996-RB, transcript variant B (wal), mRNA
0	0	3	3	2	NM_165847.1	CG8996-RA, transcript variant A (wal), mRNA
0	0	2	2	6	NM_141038.2	CG9389-RA (CG9389), mRNA
0	0	2	2	5	NM_057258.2	CG10699-RA, transcript variant A (Lim3), mRNA
0	0	2	2	5	NM_165277.1	CG10699-RB, transcript variant B (Lim3), mRNA
0	0	1	8	4	NM_078648.2	CG9901-RA (Arp14D), mRNA
0	0	1	6	3	NM_168072.1	CG15010-RA, transcript variant A (ago), mRNA
0	0	1	6	3	NM_168073.1	CG15010-RB, transcript variant B (ago), mRNA
0	0	1	6	3	NM_079198.2	CG15010-RC, transcript variant C (ago), mRNA
0	0	1	2	7	NM_001038734.1	CG16902-RC (Hr4), mRNA
0	0	1	2	6	NM_001042804.1	CG2174-RB, transcript variant B (Myo10A), mRNA
0	0	1	2	6	NM_132441.1	CG2174-RA, transcript variant A (Myo10A), mRNA
0	0	1	2	5	NM_001042881.1	CG8486-RC, transcript variant C (CG8486), mRNA
0	0	1	2	5	NM_164795.2	CG8486-RB, transcript variant B (CG8486), mRNA
0	0	1	2	5	NM_135344.2	CG8486-RA, transcript variant A (CG8486), mRNA
0	0	0	9	26	NM_079607.2	CG6174-RA (Arp87C), mRNA
0	0	0	9	20	NM_079244.2	CG7558-RA (Arp66B), mRNA

Back