NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5854R-4

Symbol CG5854 Full Name CG5854

CG No CG5854 Old CG No CG5854

Synonyms anon-WO0118547.306, CG5854

Accession No (Link to NCBI) NM_142939.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTCTGAAAAGCCGACTGTTCT

Primer Seq. 3'

aatctagaggtaccCCAGTTCCTTCTCCACCTTGA

Predicted Fragment Size

IR fragment full Seq

0001 NTCTGAAAAG CCGACTGTTC TGATTTTGGG TGGCTGCGGC TTCATTGGAC GCAACTTGGC 
0061 CACTTATCTG CTGGACAATG AACTGGCGCA GGAAATACGA CTGGCCGATA AGACCCCTCC 
0121 GCAGATGGCA TGGCTAAACG AGGAGCAGAC GCGGGTCTTC GAGAGCGATC GGGTGGAGTT 
0181 CTGCAGCGCT AACCTAATAA ACGCCGCCTC ATGCAANGGC GGCTTTTGCG CCACACCCNC 
0241 ACCACTGGCC GGGCCTGGGA CATTGTCATC AATTGTGCGG CCGAGACGCG TGCCAACCAG 
0301 GATGATGCCG TATACAAGGA GGGTATCCTG AAGCTCAGTT TGAACTGCGC CAATGAGGCG 
0361 GCTAACCAGC GGGTCAAACG ATACGTGGAA CTCAGCTCTG GTTGTGTGAA CAGCAGCGAA 
0421 AAGACGCCGT TAAAAGNGGA CTGCAAGANG GANCCCTGGA CgGGCGTgGC CAAGCAGAAG 
0481 CTCAAGGTGG AGAAGGAACT GG

in silico PCR Fragment

0001 GTCTGAAAAG CCGACTGTTC TGATTTTGGG TGGCTGCGGC TTCATTGGAC GCAACTTGGC 
0061 CACTTATCTG CTGGACAATG AACTGGCGCA GGAAATACGA CTGGCCGATA AGACCCCTCC 
0121 GCAGATGGCA TGGCTAAACG AGGAGCAGAC GCGGGTCTTC GAGAGCGATC GGGTGGAGTT 
0181 CTGCAGCGCG AACCTAATAA ACGCCGCCTC ATGCAA-GGC GGCTTTTGCG CCACACCC-C 
0241 ACCACTGGAC GGGCCTGGGA CATTGTCATC AATTGTGCGG CCGAGACGCG TGCCAACCAG 
0301 GATGATGCCG TATACAAGGA GGGTATCCTG AAGCTCAGTT TGAACTGCGC CAATGAGGCG 
0361 GCTAACCAGC GGGTCAAACG ATACGTGGAA CTCAGCTCTG GTTGTGTGAA CAGCAGCGAA 
0421 AAGACGCCGT TAAAAGAGGA CTGCAAGACG GATCCCTGGA CGGGCGTGGC CAAGCAGAAG 
0481 CTCAAGGTGG AGAAGGAACT GG

Assemble Data


5854R-4.IR_full        1   TCTGAAAAGCCGACTGTTCTGATTTTGGGTGGCTGCGGCTTCATTGGACGCAACTTGGC 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5854R-4.in silico      1   TCTGAAAAGCCGACTGTTCTGATTTTGGGTGGCTGCGGCTTCATTGGACGCAACTTGGC 59


5854R-4.IR_full       61  CACTTATCTGCTGGACAATGAACTGGCGCAGGAAATACGACTGGCCGATAAGACCCCTCC 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5854R-4.in silico     61  CACTTATCTGCTGGACAATGAACTGGCGCAGGAAATACGACTGGCCGATAAGACCCCTCC 119


5854R-4.IR_full       121 GCAGATGGCATGGCTAAACGAGGAGCAGACGCGGGTCTTCGAGAGCGATCGGGTGGAGTT 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5854R-4.in silico     121 GCAGATGGCATGGCTAAACGAGGAGCAGACGCGGGTCTTCGAGAGCGATCGGGTGGAGTT 179


5854R-4.IR_full       181 CTGCAGCGCTAACCTAATAAACGCCGCCTCATGCAANGGCGGCTTTTGCGCCACACCCNC 239
                          ||||||||| |||||||||||||||||||||||||| ||||||||||||||||||||| |
5854R-4.in silico     181 CTGCAGCGCGAACCTAATAAACGCCGCCTCATGCAA-GGCGGCTTTTGCGCCACACCC-C 239


5854R-4.IR_full       241 ACCACTGGCCGGGCCTGGGACATTGTCATCAATTGTGCGGCCGAGACGCGTGCCAACCAG 299
                          |||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
5854R-4.in silico     241 ACCACTGGACGGGCCTGGGACATTGTCATCAATTGTGCGGCCGAGACGCGTGCCAACCAG 299


5854R-4.IR_full       301 GATGATGCCGTATACAAGGAGGGTATCCTGAAGCTCAGTTTGAACTGCGCCAATGAGGCG 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5854R-4.in silico     301 GATGATGCCGTATACAAGGAGGGTATCCTGAAGCTCAGTTTGAACTGCGCCAATGAGGCG 359


5854R-4.IR_full       361 GCTAACCAGCGGGTCAAACGATACGTGGAACTCAGCTCTGGTTGTGTGAACAGCAGCGAA 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5854R-4.in silico     361 GCTAACCAGCGGGTCAAACGATACGTGGAACTCAGCTCTGGTTGTGTGAACAGCAGCGAA 419


5854R-4.IR_full       421 AAGACGCCGTTAAAAGNGGACTGCAAGANGGANCCCTGGACGGGCGTGGCCAAGCAGAAG 479
                          |||||||||||||||| ||||||||||| ||| |||||||||||||||||||||||||||
5854R-4.in silico     421 AAGACGCCGTTAAAAGAGGACTGCAAGACGGATCCCTGGACGGGCGTGGCCAAGCAGAAG 479


5854R-4.IR_full       481 CTCAAGGTGGAGAAGGAACTGG 501
                          ||||||||||||||||||||||
5854R-4.in silico     481 CTCAAGGTGGAGAAGGAACTGG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	592	0	0	0	NM_170099.1	CG5854-RB, transcript variant B (CG5854), mRNA
78.71	466	2	3	2	NM_142939.1	CG5854-RA, transcript variant A (CG5854), mRNA
0.33	2	1	1	1	NM_142857.2	CG17121-RA (CG17121), mRNA
0.16	1	5	9	13	NM_079001.2	CG3886-RA (Psc), mRNA
0.16	1	2	3	2	NM_001043127.1	CG34121-RA (CG34121), mRNA
0	0	4	7	13	NM_001038734.1	CG16902-RC (Hr4), mRNA
0	0	4	2	7	NM_001043262.1	CG34157-RE, transcript variant E (Dys), mRNA
0	0	3	6	2	NM_134938.2	CG3238-RA (CG3238), mRNA
0	0	3	6	2	NM_141702.2	CG12806-RA (Teh1), mRNA
0	0	3	5	2	NM_132109.1	CG3198-RA (CG3198), mRNA
0	0	3	4	11	NM_132908.2	CG4453-RA (Nup153), mRNA
0	0	3	3	5	NM_176121.1	CG33183-RC, transcript variant C (Hr46), mRNA
0	0	3	3	3	NM_135112.1	CG11030-RA (CG11030), mRNA
0	0	3	3	2	NM_140424.1	CG9007-RA (CG9007), mRNA
0	0	3	2	10	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
0	0	3	2	10	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
0	0	3	2	5	NM_057617.3	CG10497-RA, transcript variant A (Sdc), mRNA
0	0	3	2	4	NM_132650.1	CG10617-RA (CG10617), mRNA
0	0	2	7	14	NM_143226.1	CG5467-RA (CG5467), mRNA
0	0	2	7	2	NM_079558.4	CG31453-RA (CG31453), mRNA
0	0	2	6	13	NM_139541.1	CG12017-RA (CG12017), mRNA
0	0	2	5	9	NM_079470.2	CG10564-RA (Ac78C), mRNA
0	0	2	5	4	NM_079471.2	CG18023-RA, transcript variant A (Eip78C), mRNA
0	0	2	5	4	NM_168892.1	CG18023-RB, transcript variant B (Eip78C), mRNA
0	0	2	4	2	NM_133108.2	CG32542-RA (CG32542), mRNA
0	0	2	2	3	NM_134735.1	CG4785-RA (CG4785), mRNA
0	0	1	7	4	NM_176737.1	CG9213-RA (CG9213), mRNA
0	0	1	6	28	NM_132413.1	CG15295-RA (CG15295), mRNA
0	0	1	6	5	NM_080320.2	CG3653-RB, transcript variant B (kirre), mRNA
0	0	1	6	4	NM_165746.1	CG4001-RB, transcript variant B (Pfk), mRNA

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