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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
14435R-1 |
| Symbol |
CG14435 |
Full Name |
CG14435 |
| CG No |
CG14435 |
Old CG No |
CG14435 |
| Synonyms |
CG14435 |
| Accession No (Link to NCBI) |
NM_132131.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Nicolson S, Manning JA, Lim Y, Jiang X, Kolze E, Dayan S, Umargamwala R, Xu T, Sandow JJ, Webb AI, Kumar S, Denton D.
The Drosophila ZNRF1/2 homologue, detour, interacts with HOPS complex and regulates autophagy.
Commun Biol (2024) 7(1) 183
[
PubMed ID = 38360932
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
PLoS One (2011) 6(9) e24168
[
PubMed ID = 21931660
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACCACCGGTCAGGGTTTCAAT |
| Primer Seq. 3' |
aatctagaggtaccTTGCACACGGGACACTTGATA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACCACCGGTC AGGGTTTCAA TCTGCTGCGC ACTTTGCCCG GCCTGCAGGT GTACCACAAC
0061 CAGAACTCCA CGTCCATCGA TCGCCAGCGG GCGCGGAAGT CTCAGCTCCG TGCCGGATAT
0121 ACAGCAGCAG GCGCAGCAGC AGCAGCAACA GGGATCGATC ACCTCGTCCG GCAGCAGTCC
0181 GCATTCGCAT CCGCATGCGG CGCACGGCCA TCCGGCCGTC GGTGTCACCG TATCCGTGTC
0241 CGGTAATGCG AATGCCAATA GCAACAGCAA CTTCACAGCC GCCGGGAACA ACAATGGATC
0301 CGCCATGCAG TCCTACATGC AGCAGAGACG CACTCTCGGC GATTCCATAA GGGACATGTC
0361 AATGACCGGC GGCAGCATCG CCGAGAGCAT CGCCCTGGCC GCTTCCGCCA CGTCCGCCAA
0421 TGGGATCGGT CGTGTCTACA CGGCCACCTC GCTTCCATCG CACATTTGGT CCTTCAATGG
0481 TATCAAGTGT CCCGTATGCA A |
| in silico PCR Fragment |
0001 ACCACCGGTC AGGGTTTCAA TCTGCTGCGC ACTTTGCCCG GCCTGCAGGT GTACCACAAC
0061 CAAAACTCCA CGTCCATCGA TCGCCAGCGG GCGCGG-AGT CTCAGCTCCG TGCCGGATAT
0121 ACAGCAGCAG GCGCAGCAGC AGCAGCAACA GGGATCGATC ACCTCGTCCG GCAGCAGTCC
0181 GCATTCGCAT CCGCATGCCG CGCACGGCCA TCCGGCCGTC GGTGTCACCG TATCCGTGTC
0241 CGGTAATGCG AATGCCAATA GCAACAGCAA CTTCACAGCC GCCGGGAACA ACAATGGATC
0301 CGCCATGCAG TCCTACATGC AGCAGAGACG CACTCTCGGC GATTCCATAA GGGACATGTC
0361 AATGACCGGC GGCAGCATCG CCGAGAGCAT CGCCCTGGCC GCTTCCGCCA CGTCCGCCAA
0421 TGGGATCGGT CGTGTCTACA CGGCCACCTC GCTTCCATCG CACATTTGGT CCTTCAATGG
0481 TATCAAGTGT CCCGTGTGCA A |
| Assemble Data |
14435R-1.IR_full 1 ACCACCGGTCAGGGTTTCAATCTGCTGCGCACTTTGCCCGGCCTGCAGGTGTACCACAAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14435R-1.in silico 1 ACCACCGGTCAGGGTTTCAATCTGCTGCGCACTTTGCCCGGCCTGCAGGTGTACCACAAC 60
14435R-1.IR_full 61 CAGAACTCCACGTCCATCGATCGCCAGCGGGCGCGGAAGTCTCAGCTCCGTGCCGGATAT 120
|| ||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
14435R-1.in silico 61 CAAAACTCCACGTCCATCGATCGCCAGCGGGCGCGG-AGTCTCAGCTCCGTGCCGGATAT 120
14435R-1.IR_full 121 ACAGCAGCAGGCGCAGCAGCAGCAGCAACAGGGATCGATCACCTCGTCCGGCAGCAGTCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14435R-1.in silico 121 ACAGCAGCAGGCGCAGCAGCAGCAGCAACAGGGATCGATCACCTCGTCCGGCAGCAGTCC 180
14435R-1.IR_full 181 GCATTCGCATCCGCATGCGGCGCACGGCCATCCGGCCGTCGGTGTCACCGTATCCGTGTC 240
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
14435R-1.in silico 181 GCATTCGCATCCGCATGCCGCGCACGGCCATCCGGCCGTCGGTGTCACCGTATCCGTGTC 240
14435R-1.IR_full 241 CGGTAATGCGAATGCCAATAGCAACAGCAACTTCACAGCCGCCGGGAACAACAATGGATC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14435R-1.in silico 241 CGGTAATGCGAATGCCAATAGCAACAGCAACTTCACAGCCGCCGGGAACAACAATGGATC 300
14435R-1.IR_full 301 CGCCATGCAGTCCTACATGCAGCAGAGACGCACTCTCGGCGATTCCATAAGGGACATGTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14435R-1.in silico 301 CGCCATGCAGTCCTACATGCAGCAGAGACGCACTCTCGGCGATTCCATAAGGGACATGTC 360
14435R-1.IR_full 361 AATGACCGGCGGCAGCATCGCCGAGAGCATCGCCCTGGCCGCTTCCGCCACGTCCGCCAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14435R-1.in silico 361 AATGACCGGCGGCAGCATCGCCGAGAGCATCGCCCTGGCCGCTTCCGCCACGTCCGCCAA 420
14435R-1.IR_full 421 TGGGATCGGTCGTGTCTACACGGCCACCTCGCTTCCATCGCACATTTGGTCCTTCAATGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14435R-1.in silico 421 TGGGATCGGTCGTGTCTACACGGCCACCTCGCTTCCATCGCACATTTGGTCCTTCAATGG 480
14435R-1.IR_full 481 TATCAAGTGTCCCGTATGCAA 501
||||||||||||||| |||||
14435R-1.in silico 481 TATCAAGTGTCCCGTGTGCAA 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
3 |
7 |
34 |
NM_132131.2 |
CG14435-RA (CG14435), mRNA |
| 2.28 |
11 |
13 |
42 |
232 |
NM_132004.2 |
CG4136-RA (CG4136), mRNA |
| 1.86 |
9 |
21 |
52 |
144 |
NM_142597.2 |
CG12254-RA (MED25), mRNA |
| 1.24 |
6 |
65 |
492 |
1073 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 1.24 |
6 |
65 |
492 |
1073 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 1.24 |
6 |
65 |
492 |
1073 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 1.24 |
6 |
17 |
67 |
354 |
NM_080364.3 |
CG5461-RA, transcript variant A (bun), mRNA |
| 1.24 |
6 |
17 |
64 |
345 |
NM_001042894.1 |
CG5461-RF, transcript variant F (bun), mRNA |
| 1.24 |
6 |
2 |
5 |
27 |
NM_139839.3 |
CG32381-RA (unc-13-4A), mRNA |
| 1.03 |
5 |
3 |
13 |
29 |
NM_079309.2 |
CG5654-RA (yps), mRNA |
| 0.82 |
4 |
23 |
69 |
170 |
NM_168837.1 |
CG17233-RC, transcript variant C (CG17233), mRNA |
| 0.82 |
4 |
23 |
69 |
170 |
NM_140939.2 |
CG17233-RB, transcript variant B (CG17233), mRNA |
| 0.82 |
4 |
23 |
66 |
108 |
NM_206483.1 |
CG17233-RB, transcript variant B (CG17233), mRNA, sub-group O CG3143-RB, transcript variant B (foxo), mRNA |
| 0.82 |
4 |
23 |
66 |
108 |
NM_206482.1 |
CG17233-RB, transcript variant B (CG17233), mRNA, sub-group O CG3143-RB, transcript variant B (foxo), mRNA, sub-group O CG3143-RC, transcript variant C (foxo), mRNA |
| 0.82 |
4 |
23 |
66 |
108 |
NM_142073.3 |
CG17233-RB, transcript variant B (CG17233), mRNA, sub-group O CG3143-RB, transcript variant B (foxo), mRNA, sub-group O CG3143-RC, transcript variant C (foxo), mRNA, sub-group O CG3143-RA, transcript variant A (foxo), mRNA |
| 0.82 |
4 |
22 |
57 |
140 |
NM_168836.1 |
CG17233-RA, transcript variant A (CG17233), mRNA |
| 0.82 |
4 |
12 |
65 |
164 |
NM_001043134.1 |
CG7368-RB (CG7368), mRNA |
| 0.82 |
4 |
7 |
33 |
86 |
NM_168444.1 |
CG11799-RB, transcript variant B (Mnf), mRNA |
| 0.82 |
4 |
7 |
33 |
86 |
NM_168445.1 |
CG11799-RC, transcript variant C (Mnf), mRNA |
| 0.82 |
4 |
7 |
33 |
86 |
NM_168446.1 |
CG11799-RD, transcript variant D (Mnf), mRNA |
| 0.82 |
4 |
7 |
33 |
86 |
NM_140183.1 |
CG11799-RE, transcript variant E (Mnf), mRNA |
| 0.82 |
4 |
7 |
33 |
86 |
NM_168443.1 |
CG11799-RA, transcript variant A (Mnf), mRNA |
| 0.82 |
4 |
7 |
25 |
41 |
NM_131968.2 |
CG32772-RA (CG32772), mRNA |
| 0.62 |
3 |
41 |
192 |
480 |
NM_166030.1 |
CG8118-RB, transcript variant B (mam), mRNA |
| 0.62 |
3 |
41 |
192 |
480 |
NM_080376.1 |
CG8118-RA, transcript variant A (mam), mRNA |
| 0.62 |
3 |
24 |
116 |
302 |
NM_169781.1 |
CG31243-RE, transcript variant E (cpo), mRNA |
| 0.62 |
3 |
24 |
116 |
302 |
NM_169782.1 |
CG31243-RA, transcript variant A (cpo), mRNA |
| 0.62 |
3 |
24 |
116 |
300 |
NM_169783.1 |
CG31243-RB, transcript variant B (cpo), mRNA |
| 0.62 |
3 |
21 |
63 |
250 |
NM_080105.2 |
CG31243-RF, transcript variant F (cpo), mRNA |
| 0.62 |
3 |
21 |
43 |
124 |
NM_130717.3 |
CG32782-RC, transcript variant C (tlk), mRNA |
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