NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 11966R-1

Symbol CG11966 Full Name CG11966

CG No CG11966 Old CG No CG11966

Synonyms CG11966

Accession No (Link to NCBI) NM_141591.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTATCTGCTCGATGGACACAA

Primer Seq. 3'

aatctagaggtaccAGCTATCGGCCACGGAGTAAA

Predicted Fragment Size

IR fragment full Seq

0001 GTATCTGCTC GATGGACACA AGCTGTTGCT GGAGCATGAC CTGGACTCCC TGCCAAGTGA 
0061 TGCGGATCAG AAAAACTCGC TGGACGTGCT GGACAATCTG CTGCTGAATG GATCGTCGCT 
0121 GAATGCGCTG TCGGATCTAA AGCCACTGCC GCCCTTCACA GGCTACACGG GCCACCTGTC 
0181 CATCAATGGG ATCTCCGGCC ACCATTATCA TGCCATTGCC CAGAGACTGC CGGATGAGAG 
0241 CAACAACAAC TACATGCAGA GTGCCTATCA CCCGCAGAAC CAATCGAATC CCACATCCAC 
0301 CACGCAATCG AATGGGGGCA GCAATAGCAA CTCCAACAAC TCCAATGAGC ACAACATAGT 
0361 ATCCTCCTCC ACCTGCTTAC CAGAAAGTGT ACTCAGCGGG AGCGGCGGAG GAGGAGGTGG 
0421 CAACGATGCC TGcCNGGCGG ACGTGAAGCT CTTCGCCGAT AGTCAACTAG ATTCTAAGCT 
0481 TTACTCCGTG GCCGATAGCT

in silico PCR Fragment

0001 GTATCTGCTC GATGGACACA AGCTGTTGCT GGAGCATGAC CTGGACTCCC TGCCAAGTGA 
0061 TGCGGATCAG AAAAACTCGC TGGACGTGCT GGACAATCTG CTGCTGAATG GATCGTCGCT 
0121 GAATGCGCTG TCGGATCTAA AGCCACTGCC GCCCTTCACA GGCTACACGG GCCACCTGTC 
0181 CATCAATGGG ATCTCCGGCC ACCATTATCA TGCCATTGCC CAGAGACTGC CGGATGAGAG 
0241 CAACAACAAC TACATGCAGA GTGCCTATCA CCCGCAGAAC CAATCGAATC CCACATCCAC 
0301 CACGCAATCG AATGGGGGCA GCAATAGCAA CTCCAACAAC TCCAATGAGC ACAACATAGT 
0361 ATCCTCCTCC ACCTGCTTAC CAGAAAGTGT ACTCAGCGGG AGCGGCGGAG GAGGAGGTGG 
0421 CAACGATGCC TGCCTGGCGG ACGTGAAGCT CTTCGCCGAT AGTCAACTAG ATTCTAAGCT 
0481 TTACTCCGTG GCCGATAGCT

Assemble Data


11966R-1.IR_full       1   GTATCTGCTCGATGGACACAAGCTGTTGCTGGAGCATGACCTGGACTCCCTGCCAAGTGA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11966R-1.in silico     1   GTATCTGCTCGATGGACACAAGCTGTTGCTGGAGCATGACCTGGACTCCCTGCCAAGTGA 60


11966R-1.IR_full       61  TGCGGATCAGAAAAACTCGCTGGACGTGCTGGACAATCTGCTGCTGAATGGATCGTCGCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11966R-1.in silico     61  TGCGGATCAGAAAAACTCGCTGGACGTGCTGGACAATCTGCTGCTGAATGGATCGTCGCT 120


11966R-1.IR_full       121 GAATGCGCTGTCGGATCTAAAGCCACTGCCGCCCTTCACAGGCTACACGGGCCACCTGTC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11966R-1.in silico     121 GAATGCGCTGTCGGATCTAAAGCCACTGCCGCCCTTCACAGGCTACACGGGCCACCTGTC 180


11966R-1.IR_full       181 CATCAATGGGATCTCCGGCCACCATTATCATGCCATTGCCCAGAGACTGCCGGATGAGAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11966R-1.in silico     181 CATCAATGGGATCTCCGGCCACCATTATCATGCCATTGCCCAGAGACTGCCGGATGAGAG 240


11966R-1.IR_full       241 CAACAACAACTACATGCAGAGTGCCTATCACCCGCAGAACCAATCGAATCCCACATCCAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11966R-1.in silico     241 CAACAACAACTACATGCAGAGTGCCTATCACCCGCAGAACCAATCGAATCCCACATCCAC 300


11966R-1.IR_full       301 CACGCAATCGAATGGGGGCAGCAATAGCAACTCCAACAACTCCAATGAGCACAACATAGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11966R-1.in silico     301 CACGCAATCGAATGGGGGCAGCAATAGCAACTCCAACAACTCCAATGAGCACAACATAGT 360


11966R-1.IR_full       361 ATCCTCCTCCACCTGCTTACCAGAAAGTGTACTCAGCGGGAGCGGCGGAGGAGGAGGTGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11966R-1.in silico     361 ATCCTCCTCCACCTGCTTACCAGAAAGTGTACTCAGCGGGAGCGGCGGAGGAGGAGGTGG 420


11966R-1.IR_full       421 CAACGATGCCTGCCNGGCGGACGTGAAGCTCTTCGCCGATAGTCAACTAGATTCTAAGCT 480
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
11966R-1.in silico     421 CAACGATGCCTGCCTGGCGGACGTGAAGCTCTTCGCCGATAGTCAACTAGATTCTAAGCT 480


11966R-1.IR_full       481 TTACTCCGTGGCCGATAGCT 500
                           ||||||||||||||||||||
11966R-1.in silico     481 TTACTCCGTGGCCGATAGCT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_141591.1	CG11966-RA (CG11966), mRNA
0.41	2	3	7	6	NM_132073.1	CG14445-RA (CG14445), mRNA
0.41	2	2	7	13	NM_132246.2	CG10555-RA (CG10555), mRNA
0.41	2	2	7	13	NM_142622.1	CG4360-RA (CG4360), mRNA
0.41	2	2	3	6	NM_057398.3	CG8049-RA, transcript variant A (Btk29A), mRNA
0.41	2	2	3	6	NM_164805.1	CG8049-RC, transcript variant C (Btk29A), mRNA
0.41	2	2	3	6	NM_164804.1	CG8049-RD, transcript variant D (Btk29A), mRNA
0.41	2	2	3	6	NM_057397.3	CG8049-RB, transcript variant B (Btk29A), mRNA
0.2	1	5	2	2	NM_079956.2	CG18455-RA, transcript variant A (Optix), mRNA
0.2	1	5	2	2	NM_165584.1	CG18455-RB, transcript variant B (Optix), mRNA
0.2	1	4	8	14	NM_001014706.1	CG13376-RA (CG13376), mRNA
0.2	1	2	7	12	NM_140759.2	CG5546-RA (MED19), mRNA
0.2	1	2	5	12	NM_142649.2	CG4000-RA (CG4000), mRNA
0.2	1	2	2	12	NM_141009.2	CG32432-RA (CG32432), mRNA
0	0	5	3	5	NM_206382.1	CG5151-RB, transcript variant B (CG5151), mRNA
0	0	5	3	5	NM_140587.1	CG5151-RA, transcript variant A (CG5151), mRNA
0	0	4	15	35	NM_079093.2	CG9888-RA (Fib), mRNA
0	0	4	9	33	NM_144026.1	CG11458-RA (CG11458), mRNA
0	0	4	4	11	NM_136202.4	CG31678-RA (CG31678), mRNA
0	0	3	10	23	NM_169653.2	CG31302-RB, transcript variant B (CG31302), mRNA
0	0	3	10	23	NM_169652.2	CG31302-RA, transcript variant A (CG31302), mRNA
0	0	3	10	23	NM_169654.2	CG31302-RC, transcript variant C (CG31302), mRNA
0	0	3	9	29	NM_142751.1	CG5778-RA (CG5778), mRNA
0	0	3	6	7	NM_080124.3	CG31695-RA (scw), mRNA
0	0	3	5	17	NM_206250.2	CG16973-RC, transcript variant C (msn), mRNA
0	0	3	5	17	NM_079940.4	CG16973-RA, transcript variant A (msn), mRNA
0	0	3	5	17	NM_206251.2	CG16973-RD, transcript variant D (msn), mRNA
0	0	3	5	17	NM_206252.2	CG16973-RB, transcript variant B (msn), mRNA
0	0	3	4	11	NM_133012.2	CG32560-RA (CG32560), mRNA
0	0	3	4	3	NM_139646.1	CG12607-RB (CG12607), mRNA

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