|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10066R-7 |
| Symbol |
Fer1 |
Full Name |
48 related 1 |
| CG No |
CG33323 |
Old CG No |
CG10066 |
| Synonyms |
CG10066, CG33323, BcDNA:RH30329, CG14611, Fer1 |
| Accession No (Link to NCBI) |
NM_206455.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Bauke AC, Sasse S, Matzat T, Klämbt C.
A transcriptional network controlling glial development in the Drosophila visual system.
Development (2015) 142(12) 2184-93
[
PubMed ID = 26015542
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAATTTCGACTTGGAGGCCAC |
| Primer Seq. 3' |
aatctagaggtaccGGATAATTTTCTTGGGCGGCT |
| Predicted Fragment Size |
476 |
| IR fragment full Seq |
0001 CAATTTCGAC TTGGAGGCCA CGATGGCGCG CCACTTCTTC GAGGGATCGC AGGCCACCAA
0061 CGCCTCCACC TCCAGCTCCG ACTATTTTTT TTGGGGACGA GCACAGCTCG GAGAGCGATG
0121 ACGAGGACGA TGCCTATAGC AGTGGCTTCA ACAGCGACCA GGAGGAACAC CGAGAAGACC
0181 CGGCGGAGCC ACAAGCCCCG GCGCTTGAAG TGCGCCTCCC AAATGGCCCA GCAGCGGCAG
0241 GCGGCCAATC TCCGCGAGCG CCGCCGGATG CAGAGCATCA ACGAGGCGTT CGAGGGTCTG
0301 CGCACCCACA TTCCCACGCT GCCCTACGAG AAGCGACTAA GCAAGGTGGA CACACTCAAG
0361 CTGGCCATAA GCTACATAAC CTTCCTCAGC GAGATGGTCA AGAAGGACAA GAACGGCAAC
0421 GAGCCGGGCC TAAGTCTGCA GCGTAACTAC CAAAAGGAGC CGCCCAAGAA AATTATCC |
| in silico PCR Fragment |
0001 CAATTTCGAC TTGGAGGCCA CGATGGCGCG CCACTTCTTC GAGGGATCGC AGGCCACCAA
0061 CGCCTCCACC TCCAGCTCCG ACTA-TTTTT TTGGGGACGA GCACAGCTCG GAGAGCGATG
0121 ACGAGGACGA TGCCTATAGC AGTGGCTTCA ACAGCGACCA GGA-GAACAC CGAGAAGACC
0181 CGGCGGAGCC ACAAGCCCCG GCGCTTGAAG TGCGCCTCCC AAATGGCCCA GCAGCGGCAG
0241 GCGGCCAATC TCCGCGAGCG CCGCCGGATG CAGAGCATCA ACGAGGCGTT CGAGGGTCTG
0301 CGCACCCACA TTCCCACGCT GCCCTACGAG AAGCGACTAA GCAAGGTGGA CACACTCAAG
0361 CTGGCCATAA GCTACATAAC CTTCCTCAGC GAGATGGTCA AGAAGGACAA GAACGGCAAC
0421 GAGCCGGGCC TAAGTCTGCA GCGTAACTAC CAAAAGGAGC CGCCCAAGAA AATTATCC |
| Assemble Data |
10066R-7.IR_full 1 CAATTTCGACTTGGAGGCCACGATGGCGCGCCACTTCTTCGAGGGATCGCAGGCCACCAA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-7.in silico 1 CAATTTCGACTTGGAGGCCACGATGGCGCGCCACTTCTTCGAGGGATCGCAGGCCACCAA 60
10066R-7.IR_full 61 CGCCTCCACCTCCAGCTCCGACTATTTTTTTTGGGGACGAGCACAGCTCGGAGAGCGATG 120
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
10066R-7.in silico 61 CGCCTCCACCTCCAGCTCCGACTA-TTTTTTTGGGGACGAGCACAGCTCGGAGAGCGATG 120
10066R-7.IR_full 121 ACGAGGACGATGCCTATAGCAGTGGCTTCAACAGCGACCAGGAGGAACACCGAGAAGACC 180
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
10066R-7.in silico 121 ACGAGGACGATGCCTATAGCAGTGGCTTCAACAGCGACCAGGA-GAACACCGAGAAGACC 180
10066R-7.IR_full 181 CGGCGGAGCCACAAGCCCCGGCGCTTGAAGTGCGCCTCCCAAATGGCCCAGCAGCGGCAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-7.in silico 181 CGGCGGAGCCACAAGCCCCGGCGCTTGAAGTGCGCCTCCCAAATGGCCCAGCAGCGGCAG 240
10066R-7.IR_full 241 GCGGCCAATCTCCGCGAGCGCCGCCGGATGCAGAGCATCAACGAGGCGTTCGAGGGTCTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-7.in silico 241 GCGGCCAATCTCCGCGAGCGCCGCCGGATGCAGAGCATCAACGAGGCGTTCGAGGGTCTG 300
10066R-7.IR_full 301 CGCACCCACATTCCCACGCTGCCCTACGAGAAGCGACTAAGCAAGGTGGACACACTCAAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-7.in silico 301 CGCACCCACATTCCCACGCTGCCCTACGAGAAGCGACTAAGCAAGGTGGACACACTCAAG 360
10066R-7.IR_full 361 CTGGCCATAAGCTACATAACCTTCCTCAGCGAGATGGTCAAGAAGGACAAGAACGGCAAC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-7.in silico 361 CTGGCCATAAGCTACATAACCTTCCTCAGCGAGATGGTCAAGAAGGACAAGAACGGCAAC 420
10066R-7.IR_full 421 GAGCCGGGCCTAAGTCTGCAGCGTAACTACCAAAAGGAGCCGCCCAAGAAAATTATCC 478
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10066R-7.in silico 421 GAGCCGGGCCTAAGTCTGCAGCGTAACTACCAAAAGGAGCCGCCCAAGAAAATTATCC 478
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
458 |
0 |
0 |
0 |
NM_206455.1 |
CG33323-RA (Fer1), mRNA |
| 0.87 |
4 |
2 |
3 |
2 |
NM_132240.2 |
CG10965-RA (Corp), mRNA |
| 0.43 |
2 |
2 |
3 |
2 |
NM_140059.2 |
CG3448-RB (CG3448), mRNA |
| 0.21 |
1 |
5 |
3 |
5 |
NM_166623.1 |
CG4051-RA (egl), mRNA |
| 0 |
0 |
4 |
5 |
2 |
NM_133112.1 |
CG7349-RA (CG7349), mRNA |
| 0 |
0 |
4 |
4 |
5 |
NM_135106.1 |
CG7236-RA (CG7236), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_057502.2 |
CG1322-RB, transcript variant B (zfh1), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_170522.2 |
CG1322-RC, transcript variant C (zfh1), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_170485.1 |
CG2139-RC, transcript variant C (aralar1), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_170487.1 |
CG2139-RB, transcript variant B (aralar1), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_143538.2 |
CG2139-RA, transcript variant A (aralar1), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_170486.1 |
CG2139-RD, transcript variant D (aralar1), mRNA |
| 0 |
0 |
3 |
3 |
9 |
NM_142457.2 |
CG7669-RA (CG7669), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_135861.1 |
CG15287-RA (CG15287), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_164522.1 |
CG17257-RA, transcript variant A (CG17257), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_134897.2 |
CG17257-RB, transcript variant B (CG17257), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_166527.1 |
CG5820-RA, transcript variant A (Gp150), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_166528.1 |
CG5820-RB, transcript variant B (Gp150), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_166529.1 |
CG5820-RC, transcript variant C (Gp150), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_057701.3 |
CG5820-RD, transcript variant D (Gp150), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_165285.1 |
CG31797-RA (CG31797), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_078498.2 |
CG4336-RA (rux), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_144393.1 |
CG18747-RA (CG18747), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_078604.3 |
CG32592-RA (hiw), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_168038.1 |
CG32264-RD, transcript variant D (CG32264), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_134577.1 |
CG1529-RA (CG1529), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_142053.1 |
CG14366-RA (CG14366), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132396.1 |
CG2222-RA (Psf3), mRNA |
| 0 |
0 |
1 |
7 |
9 |
NM_080049.2 |
CG11614-RA (nkd), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_057757.3 |
CG2714-RA, transcript variant A (crm), mRNA |
|
|
|
