NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10013R-1

Symbol

CG10013

Full Name

CG10013

CG No

CG10013

Old CG No

CG10013

Synonyms

CG10013

Accession No (Link to NCBI)

NM_141918.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACCCCTACTGGTCGAGAGTTC

Primer Seq. 3'

aatctagaggtaccCGATGGTCTTGACTCCGTTGC

Predicted Fragment Size

IR fragment full Seq

0001 ACCCCTACTG GTCGAGAGTT CCAATGTGTC CCAATTGATA GAGGTCAGCC CCAATGGGAT 
0061 CAGTTGGCAC GAGAAGAACG TGGAGTACCG CATCATAATG GACGAGTTCT TCGATATAAT 
0121 CACCAGGACG AATGCTGGCG ACGTGCCCAT TACCTTAGTC GAAATGAAGG GTAGTCATGT 
0181 GTCATTTGGA TTATATCTCC GTAGGATTAG GTACAGCATT TTGGATGCGC TCACCATTAA 
0241 TGTGGAACTG GGGGAGCTGC CAATGTGTAT TTCCCCATAC AATTCCTGTT CCCTGCACTG 
0301 CAGCAACTGT ACGAATGAAA TCATTGGGCA AAGGCAGTAT TATCATATCC AGGAGGTTCC 
0361 GATAACGACC ATCTTGCCGC AGAACTACTT CTGTCCCCGC AACAGGATCC CAGTGTATCC 
0421 TTCGGAGGAG GAGCTCTATT ACGGCCTCAA CTATCTGGTC GTATGCTCCG AGCTGCTCGG 
0481 CAACGGAGTC AAGACCATCG

in silico PCR Fragment

0001 ACCCCTACTG GTCGAGAGTT CCAATGTGTC CCAATTGATA GAGGTCAGCC CCAATGGGAT 
0061 CAGTTGGCAC GAGAAGAACG TGGAGTACCG CATCATAATG GACGAGTTCT TCGATATAAT 
0121 CACCAGGACG AATGCTGGCG ACGTGCCCAT TACCTTAGTC GAAATGAAGG GTAGTCATGT 
0181 GTCATTTGGA TTATATCTCC GTAGGATTAG GTACAGCATT TTGGATGCGC TCACCATTAA 
0241 TGTGGAACTG GGGGAGCTGC CAATGTGTAT TTCCCCATAC AATTCCTGTT CCCTGCACTG 
0301 CAGCAACTGT ACGAATGAAA TCATTGGGCA AAGGCAGTAT TATCATATCC AGGAGGTTCC 
0361 GATAACGACC ATCTTGCCGC AGAACTACTT CTGTCCCCGC AACAGGATCC CAGTGTATCC 
0421 TTCGGAGGAG GAGCTCTATT ACGGCCTCAA CTATCTGGTC GTATGCTCCG AGCTGCTCGG 
0481 CAACGGAGTC AAGACCATCG

Assemble Data


10013R-1.IR_full       1   ACCCCTACTGGTCGAGAGTTCCAATGTGTCCCAATTGATAGAGGTCAGCCCCAATGGGAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10013R-1.in silico     1   ACCCCTACTGGTCGAGAGTTCCAATGTGTCCCAATTGATAGAGGTCAGCCCCAATGGGAT 60


10013R-1.IR_full       61  CAGTTGGCACGAGAAGAACGTGGAGTACCGCATCATAATGGACGAGTTCTTCGATATAAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10013R-1.in silico     61  CAGTTGGCACGAGAAGAACGTGGAGTACCGCATCATAATGGACGAGTTCTTCGATATAAT 120


10013R-1.IR_full       121 CACCAGGACGAATGCTGGCGACGTGCCCATTACCTTAGTCGAAATGAAGGGTAGTCATGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10013R-1.in silico     121 CACCAGGACGAATGCTGGCGACGTGCCCATTACCTTAGTCGAAATGAAGGGTAGTCATGT 180


10013R-1.IR_full       181 GTCATTTGGATTATATCTCCGTAGGATTAGGTACAGCATTTTGGATGCGCTCACCATTAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10013R-1.in silico     181 GTCATTTGGATTATATCTCCGTAGGATTAGGTACAGCATTTTGGATGCGCTCACCATTAA 240


10013R-1.IR_full       241 TGTGGAACTGGGGGAGCTGCCAATGTGTATTTCCCCATACAATTCCTGTTCCCTGCACTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10013R-1.in silico     241 TGTGGAACTGGGGGAGCTGCCAATGTGTATTTCCCCATACAATTCCTGTTCCCTGCACTG 300


10013R-1.IR_full       301 CAGCAACTGTACGAATGAAATCATTGGGCAAAGGCAGTATTATCATATCCAGGAGGTTCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10013R-1.in silico     301 CAGCAACTGTACGAATGAAATCATTGGGCAAAGGCAGTATTATCATATCCAGGAGGTTCC 360


10013R-1.IR_full       361 GATAACGACCATCTTGCCGCAGAACTACTTCTGTCCCCGCAACAGGATCCCAGTGTATCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10013R-1.in silico     361 GATAACGACCATCTTGCCGCAGAACTACTTCTGTCCCCGCAACAGGATCCCAGTGTATCC 420


10013R-1.IR_full       421 TTCGGAGGAGGAGCTCTATTACGGCCTCAACTATCTGGTCGTATGCTCCGAGCTGCTCGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10013R-1.in silico     421 TTCGGAGGAGGAGCTCTATTACGGCCTCAACTATCTGGTCGTATGCTCCGAGCTGCTCGG 480


10013R-1.IR_full       481 CAACGGAGTCAAGACCATCG 500
                           ||||||||||||||||||||
10013R-1.in silico     481 CAACGGAGTCAAGACCATCG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_141918.1	CG10013-RA (CG10013), mRNA
0	0	1	3	2	NM_001014565.1	CG10491-RB, transcript variant B (vn), mRNA
0	0	1	3	2	NM_079218.2	CG10491-RA, transcript variant A (vn), mRNA
0	0	0	5	5	NM_141785.1	CG4706-RA (CG4706), mRNA
0	0	0	5	3	NM_142334.1	CG3995-RA (CG3995), mRNA
0	0	0	5	2	NM_133107.1	CG7326-RA (CG7326), mRNA
0	0	0	4	7	NM_136538.2	CG30359-RA (CG30359), mRNA
0	0	0	4	5	NM_140494.1	CG13457-RB, transcript variant B (CG13457), mRNA
0	0	0	4	5	NM_168610.1	CG13457-RA, transcript variant A (CG13457), mRNA
0	0	0	4	4	NM_138261.2	CG9166-RA (312), mRNA
0	0	0	4	3	NM_079052.2	CG4909-RA (POSH), mRNA
0	0	0	4	3	NM_164950.1	CG31723-RA (CG31723), mRNA
0	0	0	4	2	NM_132713.1	CG11584-RB (CG11584), mRNA
0	0	0	3	7	NM_206419.1	CG32438-RD, transcript variant D (Smc5), mRNA
0	0	0	3	7	NM_141077.3	CG32438-RA, transcript variant A (Smc5), mRNA
0	0	0	3	7	NM_079505.2	CG1119-RA, transcript variant A (Gnf1), mRNA
0	0	0	3	7	NM_168924.2	CG32438-RB, transcript variant B (Smc5), mRNA
0	0	0	3	7	NM_001014605.1	CG1119-RB, transcript variant B (Gnf1), mRNA
0	0	0	3	5	NM_143699.2	CG11352-RC, transcript variant C (jim), mRNA
0	0	0	3	5	NM_001014601.1	CG11352-RD, transcript variant D (jim), mRNA
0	0	0	3	5	NM_168964.2	CG11352-RB, transcript variant B (jim), mRNA
0	0	0	3	4	NM_132430.2	CG11207-RA (feo), mRNA
0	0	0	3	4	NM_139691.1	CG10673-RA (CG10673), mRNA
0	0	0	3	3	NM_166134.2	CG8395-RA (Rrp42), mRNA
0	0	0	3	3	NM_141925.1	CG10035-RA (CG10035), mRNA
0	0	0	3	2	NM_165891.1	CG30045-RA (CG30045), mRNA
0	0	0	3	2	NM_001031999.1	CG17117-RF, transcript variant F (hth), mRNA
0	0	0	3	2	NM_141706.1	CG5358-RA (Art4), mRNA
0	0	0	2	8	NM_169704.1	CG32855-RA (CG32855), mRNA
0	0	0	2	6	NM_057357.2	CG1569-RA (rod), mRNA

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