National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock discarded request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10006R-2 
 Symbol CG10006  Full Name CG10006 
 CG No CG10006  Old CG No CG10006 
 Synonyms CG10006 
 Accession No (Link to NCBI) NM_140475.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCCGCATGCCACAAATGTAAC 
 Primer Seq. 3' 
aatctagaggtaccTGATGCTGGCATAGATCCAAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCCGCATGCC ACAAATGTAA CGTATTACTT TGAGCCGACA CGAGTCAATG CCACCGATGA 
0061 GGAAGCTTTG GAGCTGCAAA TCTCTCCTCG TGGCAATCAC TCAAATTCAC AGTCGGAGGC 
0121 CGAGGTTCCG GAGTTCCTAG AAATTCATGA CCCCAAGCAC CGACATCCGA GGGAGAGTTC 
0181 TGAACCTGTT GCCGCTTGCT TATCACCGAA GTCTTTACTT TCCCTCATCG TGAACCACAA 
0241 CGATCTCCAC AAAAACATTG CCTACCGCAA GCTGCTCATT AAGAATGACG TTAGCTCAAC 
0301 TGATGGCGTC CACAATTCTG AAGAGGAATA CAACGAATTC ATCAACTCGG TGCGGATCAC 
0361 TCCGCGAGCT TTTATGAAAC TCTGTCCAGC TCTATTAGCC CAGATCGATA ATGGAGTTTG 
0421 CAAGCAACCA GCTGTACGAT CAGTAAACCT TAACGATAAG AACCAGAAGA TATGGTACGC 
0481 TTGGATCTAT GCCAGCATCA  
 in silico PCR Fragment 
0001 GCCGCATGCC ACAAATGTAA CGTATTACTT TGAGCCGACA CGAGTCAATG CCACCGATGA 
0061 GGAAGCTTTG GAGCTGCAAA TCTCTCCTCG TGGCAATCAC TCAAATTCAC AGTCGGAGGC 
0121 CGAGGTTCCG GAGTTCCTAG AAATTCATGA CCCCAAGCAC CGACATCCGA GGGAGAGTTC 
0181 TGAACCTGTT GCCGCTTGCT TATCACCGAA GTCTTTACTT TCCCTCATCG TGAACCACAA 
0241 CGATCTCCAC AAAAACATTG CCTACCGCAA GCTGCTCATT AAGAATGACG TTAGCTCAAC 
0301 TGATGGCGTC CACAATTCTG AAGAGGAATA CAACGAATTC ATCAACTCGG TGCGGATCAC 
0361 TCCGCGAGCT TTTATGAAAC TCTGTCCAGC TCTATTAGCC CAGATCGATA ATGGAGTTTG 
0421 CAAGCAACCA GCTGTACGAT CAGTAAACCT TAACGATAAG AACCAGAAGA TATGGTACGC 
0481 TTGGATCTAT GCCAGCATCA  
 Assemble Data 

10006R-2.IR_full       1   GCCGCATGCCACAAATGTAACGTATTACTTTGAGCCGACACGAGTCAATGCCACCGATGA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10006R-2.in silico     1   GCCGCATGCCACAAATGTAACGTATTACTTTGAGCCGACACGAGTCAATGCCACCGATGA 60


10006R-2.IR_full       61  GGAAGCTTTGGAGCTGCAAATCTCTCCTCGTGGCAATCACTCAAATTCACAGTCGGAGGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10006R-2.in silico     61  GGAAGCTTTGGAGCTGCAAATCTCTCCTCGTGGCAATCACTCAAATTCACAGTCGGAGGC 120


10006R-2.IR_full       121 CGAGGTTCCGGAGTTCCTAGAAATTCATGACCCCAAGCACCGACATCCGAGGGAGAGTTC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10006R-2.in silico     121 CGAGGTTCCGGAGTTCCTAGAAATTCATGACCCCAAGCACCGACATCCGAGGGAGAGTTC 180


10006R-2.IR_full       181 TGAACCTGTTGCCGCTTGCTTATCACCGAAGTCTTTACTTTCCCTCATCGTGAACCACAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10006R-2.in silico     181 TGAACCTGTTGCCGCTTGCTTATCACCGAAGTCTTTACTTTCCCTCATCGTGAACCACAA 240


10006R-2.IR_full       241 CGATCTCCACAAAAACATTGCCTACCGCAAGCTGCTCATTAAGAATGACGTTAGCTCAAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10006R-2.in silico     241 CGATCTCCACAAAAACATTGCCTACCGCAAGCTGCTCATTAAGAATGACGTTAGCTCAAC 300


10006R-2.IR_full       301 TGATGGCGTCCACAATTCTGAAGAGGAATACAACGAATTCATCAACTCGGTGCGGATCAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10006R-2.in silico     301 TGATGGCGTCCACAATTCTGAAGAGGAATACAACGAATTCATCAACTCGGTGCGGATCAC 360


10006R-2.IR_full       361 TCCGCGAGCTTTTATGAAACTCTGTCCAGCTCTATTAGCCCAGATCGATAATGGAGTTTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10006R-2.in silico     361 TCCGCGAGCTTTTATGAAACTCTGTCCAGCTCTATTAGCCCAGATCGATAATGGAGTTTG 420


10006R-2.IR_full       421 CAAGCAACCAGCTGTACGATCAGTAAACCTTAACGATAAGAACCAGAAGATATGGTACGC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10006R-2.in silico     421 CAAGCAACCAGCTGTACGATCAGTAAACCTTAACGATAAGAACCAGAAGATATGGTACGC 480


10006R-2.IR_full       481 TTGGATCTATGCCAGCATCA 500
                           ||||||||||||||||||||
10006R-2.in silico     481 TTGGATCTATGCCAGCATCA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140475.1  CG10006-RA (CG10006), mRNA 
0.2   1  2  6  5  NM_165818.1  CG30033-RB (CG30033), mRNA 
0   0  3  5  2  NM_139701.1  CG4769-RA (CG4769), mRNA 
0   0  3  2  5  NM_001038858.1  CG8585-RB, transcript variant B (Ih), mRNA 
0   0  3  2  5  NM_001038859.1  CG8585-RE, transcript variant E (Ih), mRNA 
0   0  3  2  5  NM_001038856.1  CG8585-RC, transcript variant C (Ih), mRNA 
0   0  3  2  4  NM_001038857.1  CG8585-RD, transcript variant D (Ih), mRNA 
0   0  2  4  7  NM_135957.2  CG31738-RB, transcript variant B (CG31738), mRNA 
0   0  1  5  3  NM_131923.2  CG6121-RA (Tip60), mRNA 
0   0  1  2  4  NM_058161.3  CG5595-RA (Sce), mRNA 
0   0  0  5  6  NM_140842.1  CG9629-RA (CG9629), mRNA 
0   0  0  5  3  NM_080713.3  CG10746-RA (fok), mRNA 
0   0  0  4  3  NM_169846.1  CG31043-RB, transcript variant B (gukh), mRNA 
0   0  0  4  3  NM_001032021.1  CG31043-RC, transcript variant C (gukh), mRNA 
0   0  0  4  3  NM_169845.1  CG31043-RA, transcript variant A (gukh), mRNA 
0   0  0  4  2  NM_141732.1  CG3999-RA (CG3999), mRNA 
0   0  0  4  2  NM_137570.1  CG10051-RA (CG10051), mRNA 
0   0  0  3  8  NM_168753.1  CG5535-RB, transcript variant B (CG5535), mRNA 
0   0  0  3  8  NM_140762.1  CG5535-RA, transcript variant A (CG5535), mRNA 
0   0  0  3  6  NM_176229.1  CG33147-RA (CG33147), mRNA 
0   0  0  3  6  NM_138036.2  CG3209-RA, transcript variant A (CG3209), mRNA 
0   0  0  3  6  NM_143380.3  CG1520-RA, transcript variant A (WASp), mRNA 
0   0  0  3  6  NM_170378.2  CG1520-RB, transcript variant B (WASp), mRNA 
0   0  0  3  6  NM_176578.1  CG1520-RC, transcript variant C (WASp), mRNA 
0   0  0  3  5  NM_132267.4  CG12109-RB (Caf1-180), mRNA 
0   0  0  3  5  NM_132849.1  CG15602-RA (CG15602), mRNA 
0   0  0  3  4  NM_165011.2  CG31764-RB, transcript variant B (vir-1), mRNA 
0   0  0  3  4  NM_140821.2  CG3819-RA (CG3819), mRNA 
0   0  0  3  4  NM_165010.2  CG31764-RA, transcript variant A (vir-1), mRNA 
0   0  0  3  4  NM_143367.2  CG10011-RA (CG10011), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
++ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.