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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10001R-1 |
| Symbol |
AR-2 |
Full Name |
Allatostatin Receptor 2 |
| CG No |
CG10001 |
Old CG No |
CG10001 |
| Synonyms |
CG10001, D.AlstR2, DAR-2, DAR, anon-WO0170980.116, anon-WO0170980.115, anon-WO0131005.7, AR-2 |
| Accession No (Link to NCBI) |
NM_079820.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCGCCATCACGGGATTCTTCG |
| Primer Seq. 3' |
aatctagaggtaccGCTGATGAAGAAGGTGACCTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TCGCCATCAC GGGATTCTTC GGCAACCTGC TGGTCATCCT GGTGGTGGTC TTCAACAACA
0061 ACATGCGCTC CACCACCAAC CTGATGATTG TCAATCTGGC TGCCGCTGAT CTGATGTTCG
0121 TAATCCTCTG CATTCCCTTC ACGGCCACCG ATTACATGGT GTACTACTGG CCATATGGAA
0181 GGTTCTGGTG CCGCAGTGTC CAGTACCTGA TTGTGGTGAC CGCCTTCGCC TCCATCTACA
0241 CGCTGGTGCT AATGTCCATC GATCGGTTCC TGGCGGTGGT TCATCCCATT CGCTCGCGGA
0301 TGATGAGGAC GGAGAACATT ACCCTGATTG CCATCGTGAC TCTGTGGATC GTGGTGCTGG
0361 TCGTTTCGGT GCCAGTGGCC TTCACCCACG ACGTGGTGGT GGATTACGAT GCAAAGAAGA
0421 ACATCACCTA CGGCATGTGC ACCTTCACGA CGAACGACTT CCTTGGTCCG CGCACCTACC
0481 AGGTCACCTT CTTCATCAGC |
| in silico PCR Fragment |
0001 TCGCCATCAC GGGATTCTTC GGCAACCTGC TGGTCATCCT GGTGGTGGTC TTCAACAACA
0061 ACATGCGCTC CACCACCAAC CTGATGATTG TCAATCTGGC TGCCGCTGAT CTGATGTTCG
0121 TAATCCTCTG CATTCCCTTC ACGGCCACCG ATTACATGGT GTACTACTGG CCATATGGAA
0181 GGTTCTGGTG CCGCAGTGTC CAGTACCTGA TTGTGGTGAC CGCCTTCGCC TCCATTTACA
0241 CGCTGGTGCT GATGTCCATC GATCGGTTCC TGGCGGTGGT TCATCCCATT CGCTCGCGGA
0301 TGATGAGGAC GGAGAACATT ACCCTGATTG CCATCGTGAC TCTGTGGATC GTGGTGCTGG
0361 TCGTTTCGGT GCCAGTGGCC TTCACCCACG ACGTGGTGGT GGATTACGAT GCAAAGAAGA
0421 ACATCACCTA CGGCATGTGC ACCTTCACGA CGAACGACTT CCTTGGTCCG CGCACCTACC
0481 AGGTCACCTT CTTCATCAGC |
| Assemble Data |
10001R-1.IR_full 1 TCGCCATCACGGGATTCTTCGGCAACCTGCTGGTCATCCTGGTGGTGGTCTTCAACAACA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10001R-1.in silico 1 TCGCCATCACGGGATTCTTCGGCAACCTGCTGGTCATCCTGGTGGTGGTCTTCAACAACA 60
10001R-1.IR_full 61 ACATGCGCTCCACCACCAACCTGATGATTGTCAATCTGGCTGCCGCTGATCTGATGTTCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10001R-1.in silico 61 ACATGCGCTCCACCACCAACCTGATGATTGTCAATCTGGCTGCCGCTGATCTGATGTTCG 120
10001R-1.IR_full 121 TAATCCTCTGCATTCCCTTCACGGCCACCGATTACATGGTGTACTACTGGCCATATGGAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10001R-1.in silico 121 TAATCCTCTGCATTCCCTTCACGGCCACCGATTACATGGTGTACTACTGGCCATATGGAA 180
10001R-1.IR_full 181 GGTTCTGGTGCCGCAGTGTCCAGTACCTGATTGTGGTGACCGCCTTCGCCTCCATCTACA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
10001R-1.in silico 181 GGTTCTGGTGCCGCAGTGTCCAGTACCTGATTGTGGTGACCGCCTTCGCCTCCATTTACA 240
10001R-1.IR_full 241 CGCTGGTGCTAATGTCCATCGATCGGTTCCTGGCGGTGGTTCATCCCATTCGCTCGCGGA 300
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
10001R-1.in silico 241 CGCTGGTGCTGATGTCCATCGATCGGTTCCTGGCGGTGGTTCATCCCATTCGCTCGCGGA 300
10001R-1.IR_full 301 TGATGAGGACGGAGAACATTACCCTGATTGCCATCGTGACTCTGTGGATCGTGGTGCTGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10001R-1.in silico 301 TGATGAGGACGGAGAACATTACCCTGATTGCCATCGTGACTCTGTGGATCGTGGTGCTGG 360
10001R-1.IR_full 361 TCGTTTCGGTGCCAGTGGCCTTCACCCACGACGTGGTGGTGGATTACGATGCAAAGAAGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10001R-1.in silico 361 TCGTTTCGGTGCCAGTGGCCTTCACCCACGACGTGGTGGTGGATTACGATGCAAAGAAGA 420
10001R-1.IR_full 421 ACATCACCTACGGCATGTGCACCTTCACGACGAACGACTTCCTTGGTCCGCGCACCTACC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10001R-1.in silico 421 ACATCACCTACGGCATGTGCACCTTCACGACGAACGACTTCCTTGGTCCGCGCACCTACC 480
10001R-1.IR_full 481 AGGTCACCTTCTTCATCAGC 500
||||||||||||||||||||
10001R-1.in silico 481 AGGTCACCTTCTTCATCAGC 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
3 |
NM_079820.2 |
CG10001-RA (AR-2), mRNA |
| 1.03 |
5 |
10 |
27 |
32 |
NM_166982.1 |
CG2872-RC, transcript variant C (AlstR), mRNA |
| 1.03 |
5 |
10 |
27 |
32 |
NM_079961.2 |
CG2872-RB, transcript variant B (AlstR), mRNA |
| 0 |
0 |
3 |
4 |
12 |
NM_078681.2 |
CG32540-RA (CCKLR-17D3), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_132580.1 |
CG15729-RA (CG15729), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_168443.1 |
CG11799-RA, transcript variant A (Mnf), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_140183.1 |
CG11799-RE, transcript variant E (Mnf), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_168446.1 |
CG11799-RD, transcript variant D (Mnf), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_168444.1 |
CG11799-RB, transcript variant B (Mnf), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_168445.1 |
CG11799-RC, transcript variant C (Mnf), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_001043138.1 |
CG17672-RA (CG17672), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_168218.1 |
CG32372-RA (CG32372), mRNA |
| 0 |
0 |
1 |
3 |
10 |
NM_168778.1 |
CG3979-RB, transcript variant B (Indy), mRNA |
| 0 |
0 |
1 |
3 |
10 |
NM_168779.1 |
CG3979-RC, transcript variant C (Indy), mRNA |
| 0 |
0 |
1 |
3 |
10 |
NM_079426.2 |
CG3979-RA, transcript variant A (Indy), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_078609.2 |
CG11654-RA (Ahcy13), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132429.2 |
CG2202-RA (CG2202), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_079725.2 |
CG7050-RA (Nrx-1), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_143033.2 |
CG5807-RA (CG5807), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_168103.1 |
CG7507-RB, transcript variant B (Dhc64C), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079205.3 |
CG7507-RA, transcript variant A (Dhc64C), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_205944.1 |
CG33298-RB, transcript variant B (CG33298), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_205943.1 |
CG33298-RA, transcript variant A (CG33298), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140872.1 |
CG9330-RA (CG9330), mRNA |
| 0 |
0 |
0 |
10 |
3 |
NM_057951.2 |
CG9210-RA, transcript variant A (Ac13E), mRNA |
| 0 |
0 |
0 |
10 |
3 |
NM_001014745.1 |
CG9210-RB, transcript variant B (Ac13E), mRNA |
| 0 |
0 |
0 |
9 |
14 |
NM_132661.1 |
CG12726-RA (CG12726), mRNA |
| 0 |
0 |
0 |
8 |
4 |
NM_001014758.2 |
CG33517-RC, transcript variant C (D2R), mRNA |
| 0 |
0 |
0 |
8 |
2 |
NM_001014757.2 |
CG33517-RD, transcript variant D (D2R), mRNA |
| 0 |
0 |
0 |
8 |
2 |
NM_001014760.2 |
CG33517-RA, transcript variant A (D2R), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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