|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1004R-3 |
| Symbol |
rho |
Full Name |
rhomboid |
| CG No |
CG1004 |
Old CG No |
CG1004 |
| Synonyms |
Rho-1, CG1004, Rho1, rhomboid/veinlet, ve, rho1, rho-1, Rho, DMRHOb, RHO, rhom, RHOb, DMRHO, DRORHO, Ve, DMRHOa, rho |
| Accession No (Link to NCBI) |
NM_079159.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGTTCCACCTGGGCTTCAAT |
| Primer Seq. 3' |
aatctagaggtaccATCAGGTTGAAAACGATGGCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGTTCCACC TGGGCTTCAA TATCGTCATC CAGCTGTTCT TTGGCATTCC CCTGGAGGTG
0061 ATGCACGGCA CGGCCAGGAT CGGCGTGATC TACATGGCGG GCGTTTTTGC CGGATCCCTG
0121 GGCACCAGTG TCGTCGACTC GGAGGTCTTC CTGGTGGGCG CCAGCGGTGG CGTCTATGCC
0181 CTGTTGGCCG CTCATCTGGC CAACATTACA CTGAACTATG CGCACATGAA GAGCGCATCC
0241 ACGCAACTCG GATCCGTTGT CATCTTTGTC TCCTGCGATC TGGGCTATGC TCTCTACACC
0301 CAATACTTCG ATGGAAGCGC CTTCGCCAAG GGTCCCCAGG TGTCGTACAT TGCCCACCTG
0361 ACGGGAGCCC TGGCAGGACT AACGATCGGC TTCCTGGTGC TAAAGAACTT CGGTCATCGG
0421 GAGTACGAGC AGCTCATCTG GTGGCTAGCG CTGGGCGTCT ACTGTGCCTT CACCGTCTTC
0481 GCCATCGTTT TCAACCTGAT |
| in silico PCR Fragment |
0001 TGGTTCCACC TGGGCTTCAA TATCGTCATC CAGCTGTTTT TTGGCATTCC CCTGGAGGTG
0061 ATGCACGGCA CGGCCAGGAT CGGCGTGATC TACATGGCGG GCGTTTTTGC CGGATCCCTG
0121 GGCACCAGTG TCGTCGACTC GGAGGTCTTC CTGGTGGGCG CCAGCGGTGG CGTCTATGCC
0181 CTGTTGGCCG CACATCTGGC CAACATTACA CTGAACTATG CGCACATGAA GAGCGCATCC
0241 ACGCAACTCG GATCCGTTGT CATCTTTGTC TCCTGCGATC TGGGCTATGC TCTCTACACC
0301 CAATACTTCG ATGGAAGCGC CTTCGCCAAG GGTCCCCAGG TGTCGTACAT TGCCCACCTG
0361 ACGGGAGCCC TGGCAGGACT AACGATCGGC TTTCTGGTGC TAAAGAACTT CGGTCACCGA
0421 GAGTACGAGC AGCTCATCTG GTGGCTAGCG TTGGGCGTCT ACTGTGCCTT CACCGTCTTC
0481 GCCATCGTTT TCAACCTGAT |
| Assemble Data |
1004R-3.IR_full 1 TGGTTCCACCTGGGCTTCAATATCGTCATCCAGCTGTTCTTTGGCATTCCCCTGGAGGTG 60
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
1004R-3.in silico 1 TGGTTCCACCTGGGCTTCAATATCGTCATCCAGCTGTTTTTTGGCATTCCCCTGGAGGTG 60
1004R-3.IR_full 61 ATGCACGGCACGGCCAGGATCGGCGTGATCTACATGGCGGGCGTTTTTGCCGGATCCCTG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1004R-3.in silico 61 ATGCACGGCACGGCCAGGATCGGCGTGATCTACATGGCGGGCGTTTTTGCCGGATCCCTG 120
1004R-3.IR_full 121 GGCACCAGTGTCGTCGACTCGGAGGTCTTCCTGGTGGGCGCCAGCGGTGGCGTCTATGCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1004R-3.in silico 121 GGCACCAGTGTCGTCGACTCGGAGGTCTTCCTGGTGGGCGCCAGCGGTGGCGTCTATGCC 180
1004R-3.IR_full 181 CTGTTGGCCGCTCATCTGGCCAACATTACACTGAACTATGCGCACATGAAGAGCGCATCC 240
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
1004R-3.in silico 181 CTGTTGGCCGCACATCTGGCCAACATTACACTGAACTATGCGCACATGAAGAGCGCATCC 240
1004R-3.IR_full 241 ACGCAACTCGGATCCGTTGTCATCTTTGTCTCCTGCGATCTGGGCTATGCTCTCTACACC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1004R-3.in silico 241 ACGCAACTCGGATCCGTTGTCATCTTTGTCTCCTGCGATCTGGGCTATGCTCTCTACACC 300
1004R-3.IR_full 301 CAATACTTCGATGGAAGCGCCTTCGCCAAGGGTCCCCAGGTGTCGTACATTGCCCACCTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1004R-3.in silico 301 CAATACTTCGATGGAAGCGCCTTCGCCAAGGGTCCCCAGGTGTCGTACATTGCCCACCTG 360
1004R-3.IR_full 361 ACGGGAGCCCTGGCAGGACTAACGATCGGCTTCCTGGTGCTAAAGAACTTCGGTCATCGG 420
|||||||||||||||||||||||||||||||| ||||||||||||||||||||||| ||
1004R-3.in silico 361 ACGGGAGCCCTGGCAGGACTAACGATCGGCTTTCTGGTGCTAAAGAACTTCGGTCACCGA 420
1004R-3.IR_full 421 GAGTACGAGCAGCTCATCTGGTGGCTAGCGCTGGGCGTCTACTGTGCCTTCACCGTCTTC 480
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
1004R-3.in silico 421 GAGTACGAGCAGCTCATCTGGTGGCTAGCGTTGGGCGTCTACTGTGCCTTCACCGTCTTC 480
1004R-3.IR_full 481 GCCATCGTTTTCAACCTGAT 500
||||||||||||||||||||
1004R-3.in silico 481 GCCATCGTTTTCAACCTGAT 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079159.2 |
CG1004-RA (rho), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_132478.1 |
CG11757-RA (CG11757), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_080296.2 |
CG16785-RA (fz3), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_170158.1 |
CG5794-RB, transcript variant B (CG5794), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_143018.2 |
CG5794-RD, transcript variant D (CG5794), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_206564.1 |
CG5794-RE, transcript variant E (CG5794), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_206683.2 |
CG1725-RB, transcript variant B (dlg1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_206681.2 |
CG1725-RH, transcript variant H (dlg1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_206684.2 |
CG1725-RE, transcript variant E (dlg1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_206682.2 |
CG1725-RG, transcript variant G (dlg1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_078565.3 |
CG1725-RD, transcript variant D (dlg1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_167282.2 |
CG1725-RA, transcript variant A (dlg1), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_134493.2 |
CG14214-RA (Sec61gamma), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137997.2 |
CG5597-RA (CG5597), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137971.2 |
CG5428-RA (CG5428), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079865.3 |
CG1480-RA (bnk), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142524.2 |
CG6040-RA (CG6040), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142157.1 |
CG6974-RA (CG6974), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139913.1 |
CG13681-RA (CG13681), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141850.2 |
CG14718-RA (CG14718), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142834.2 |
CG6985-RA (CG6985), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_168755.1 |
CG8127-RB, transcript variant B (Eip75B), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_080148.2 |
CG2522-RA (Gtp-bp), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079321.2 |
CG10571-RA (ara), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136894.1 |
CG8860-RA (CG8860), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_144381.1 |
CG15378-RA (lectin-22C), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_164752.2 |
CG12789-RA, transcript variant A (CG12789), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135277.2 |
CG12789-RB, transcript variant B (CG12789), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080104.2 |
CG4032-RA (Abl), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132749.1 |
CG9521-RA (CG9521), mRNA |
|
|
|
