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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10574R-2 |
| Symbol |
I-2 |
Full Name |
Inhibitor-2 |
| CG No |
CG10574 |
Old CG No |
CG10574 |
| Synonyms |
I-2Dm, Dm I-2, CG10574, I-2, Inhibitor-2, inhibitor-2, I-2PP1 |
| Accession No (Link to NCBI) |
NM_079289.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.
Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.
Genetics (2012) 192(2) 741-52
[
PubMed ID = 22798488
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGCCCACAGCTACCCTGCAAG |
| Primer Seq. 3' |
aatctagaggtaccAAACGTCCGGAGCTGGAAGCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGCCCACAGC TACCCTGCAA ggGTATACTA AAGACTTCGC GTAgcNTCGA CAAGTCGGGA
0061 GCCAGTTTTC GCAAAAGTGC CAAGTTCGAT GAGCTGAACG TGATGCAGAC CTTCCATCCG
0121 GCCGACAAGG ACTACGGACA CATGAAAATC GATGAGCCCA AAACGCCGTA CAACTACACG
0181 GAGGGCTTCG ACGAGAACAG GGACGAGCTG GACACGGAGT TGCTAGTGGA GAAACTCCGC
0241 ATAGCGGCTN AACACACAGC CGTCGACAGA GAGCATCGAG GACGATGGTT CCTCCGGTGA
0301 TGACCAGCCC CTTAGCGAAG AGGAGCGACA ACGGCGGCGC GAATTCGANG CGACNNCGTA
0361 AGGCCCACTA TCGCGAGTTT GAGGCCGTCA AACTGGCACG AAAACTGATC CAGGAGGAGG
0421 ACGACGACGA TGACGACGAG GACAAGGGTG CGGATAGCCG GcCCTCGGGC TCGTCACAGG
0481 GcNCTTCCAG CTCCGGACGT TT |
| in silico PCR Fragment |
0001 AGCCCACAGC TACCCTGCAA GGGTATACTA AAGACTTCGC GTAGCTTCGA CAAGTCGGGA
0061 GCCAGTTTTC GCAAAAGTGC CAAGTTCGAT GAGCTGAACG TGATGCAGAC CTTCCATCCG
0121 GCCGACAAGG ACTACGGACA CATGAAAATC GATGAGCCCA AAACGCCGTA CAACTACACG
0181 GAGGGCTTCG ACGAGAACAG GGACGAGCTG GACACGGAGT TGCTAGTGGA GAAACTCCGC
0241 ATAGCGGCT- AACACACAGC CGTCGACAGA GAGCATCGAG GACGATGGTT CCTCCGGTGA
0301 TGACCAGCCC CTTAGCGAAG AGGAGCGACA ACGGCGGCGC GAATTCGA-G CGACGCCGTA
0361 AGGCCCACTA TCGCGAGTTT GAGGCCGTCA AACTGGCACG AAAACTGATC CAGGAGGAGG
0421 ACGACGACGA TGACGACGAG GACAAGGGTG CGGATAGCCG GCCCTCGGGC TCGTCACAGG
0481 GCGCTTCCAG CTCCGGACGT TT |
| Assemble Data |
10574R-2.IR_full 1 AGCCCACAGCTACCCTGCAAGGGTATACTAAAGACTTCGCGTAGCNTCGACAAGTCGGGA 60
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
10574R-2.in silico 1 AGCCCACAGCTACCCTGCAAGGGTATACTAAAGACTTCGCGTAGCTTCGACAAGTCGGGA 60
10574R-2.IR_full 61 GCCAGTTTTCGCAAAAGTGCCAAGTTCGATGAGCTGAACGTGATGCAGACCTTCCATCCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10574R-2.in silico 61 GCCAGTTTTCGCAAAAGTGCCAAGTTCGATGAGCTGAACGTGATGCAGACCTTCCATCCG 120
10574R-2.IR_full 121 GCCGACAAGGACTACGGACACATGAAAATCGATGAGCCCAAAACGCCGTACAACTACACG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10574R-2.in silico 121 GCCGACAAGGACTACGGACACATGAAAATCGATGAGCCCAAAACGCCGTACAACTACACG 180
10574R-2.IR_full 181 GAGGGCTTCGACGAGAACAGGGACGAGCTGGACACGGAGTTGCTAGTGGAGAAACTCCGC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10574R-2.in silico 181 GAGGGCTTCGACGAGAACAGGGACGAGCTGGACACGGAGTTGCTAGTGGAGAAACTCCGC 240
10574R-2.IR_full 241 ATAGCGGCTNAACACACAGCCGTCGACAGAGAGCATCGAGGACGATGGTTCCTCCGGTGA 300
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
10574R-2.in silico 241 ATAGCGGCT-AACACACAGCCGTCGACAGAGAGCATCGAGGACGATGGTTCCTCCGGTGA 300
10574R-2.IR_full 301 TGACCAGCCCCTTAGCGAAGAGGAGCGACAACGGCGGCGCGAATTCGANGCGACNNCGTA 360
|||||||||||||||||||||||||||||||||||||||||||||||| ||||| ||||
10574R-2.in silico 301 TGACCAGCCCCTTAGCGAAGAGGAGCGACAACGGCGGCGCGAATTCGA-GCGACGCCGTA 360
10574R-2.IR_full 361 AGGCCCACTATCGCGAGTTTGAGGCCGTCAAACTGGCACGAAAACTGATCCAGGAGGAGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10574R-2.in silico 361 AGGCCCACTATCGCGAGTTTGAGGCCGTCAAACTGGCACGAAAACTGATCCAGGAGGAGG 420
10574R-2.IR_full 421 ACGACGACGATGACGACGAGGACAAGGGTGCGGATAGCCGGCCCTCGGGCTCGTCACAGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10574R-2.in silico 421 ACGACGACGATGACGACGAGGACAAGGGTGCGGATAGCCGGCCCTCGGGCTCGTCACAGG 480
10574R-2.IR_full 481 GCNCTTCCAGCTCCGGACGTTT 502
|| |||||||||||||||||||
10574R-2.in silico 481 GCGCTTCCAGCTCCGGACGTTT 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
21 |
NM_079289.2 |
CG10574-RA (I-2), mRNA |
| 1.65 |
8 |
2 |
6 |
27 |
NM_167022.1 |
CG32771-RA (CG32771), mRNA |
| 0.82 |
4 |
4 |
5 |
18 |
NM_164671.1 |
CG9088-RB, transcript variant B (lid), mRNA |
| 0.82 |
4 |
4 |
5 |
18 |
NM_078762.4 |
CG9088-RA, transcript variant A (lid), mRNA |
| 0.82 |
4 |
2 |
7 |
28 |
NM_131924.2 |
CG4857-RB (CG4857), mRNA |
| 0.82 |
4 |
2 |
2 |
18 |
NM_001043220.1 |
CG34127-RA (CG34127), mRNA |
| 0.62 |
3 |
19 |
26 |
82 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0.62 |
3 |
6 |
3 |
13 |
NM_079106.2 |
CG4817-RA (Ssrp), mRNA |
| 0.62 |
3 |
5 |
3 |
51 |
NM_176498.1 |
CG9297-RA, transcript variant A (CG9297), mRNA |
| 0.62 |
3 |
5 |
2 |
38 |
NM_176497.1 |
CG9297-RB, transcript variant B (CG9297), mRNA |
| 0.41 |
2 |
9 |
11 |
36 |
NM_078981.2 |
CG8967-RA (otk), mRNA |
| 0.41 |
2 |
6 |
22 |
51 |
NM_001032052.1 |
CG33715-RE, transcript variant E (Msp-300), mRNA |
| 0.41 |
2 |
6 |
16 |
30 |
NM_132548.2 |
CG15736-RA (Chrac-16), mRNA |
| 0.41 |
2 |
3 |
5 |
9 |
NM_136906.1 |
CG13170-RA (CG13170), mRNA |
| 0.41 |
2 |
2 |
3 |
11 |
NM_170330.1 |
CG5954-RB, transcript variant B (l(3)mbt), mRNA |
| 0.41 |
2 |
2 |
3 |
11 |
NM_079805.2 |
CG5954-RA, transcript variant A (l(3)mbt), mRNA |
| 0.2 |
1 |
13 |
23 |
32 |
NM_079842.2 |
CG17958-RA (Sry-delta), mRNA |
| 0.2 |
1 |
12 |
23 |
30 |
NM_132095.2 |
CG3918-RA (CG3918), mRNA |
| 0.2 |
1 |
6 |
11 |
25 |
NM_142921.2 |
CG10225-RA (CG10225), mRNA |
| 0.2 |
1 |
6 |
6 |
31 |
NM_165735.2 |
CG12919-RA, transcript variant A (egr), mRNA |
| 0.2 |
1 |
6 |
6 |
31 |
NM_206069.1 |
CG12919-RB, transcript variant B (egr), mRNA |
| 0.2 |
1 |
5 |
8 |
34 |
NM_131944.1 |
CG3546-RA (CG3546), mRNA |
| 0.2 |
1 |
4 |
18 |
45 |
NM_137261.1 |
CG15709-RA (CG15709), mRNA |
| 0.2 |
1 |
4 |
12 |
32 |
NM_143801.2 |
CG5935-RB, transcript variant B (Dek), mRNA |
| 0.2 |
1 |
4 |
12 |
32 |
NM_166198.1 |
CG5935-RC, transcript variant C (Dek), mRNA |
| 0.2 |
1 |
4 |
12 |
32 |
NM_206139.2 |
CG5935-RD, transcript variant D (Dek), mRNA |
| 0.2 |
1 |
4 |
12 |
32 |
NM_166199.2 |
CG5935-RA, transcript variant A (Dek), mRNA |
| 0.2 |
1 |
2 |
2 |
6 |
NM_139419.1 |
CG13925-RA (CG13925), mRNA |
| 0 |
0 |
15 |
20 |
41 |
NM_141852.1 |
CG12594-RA (CG12594), mRNA |
| 0 |
0 |
12 |
12 |
22 |
NM_078601.2 |
CG9533-RA (rut), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| +- |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| + |
|
| VB |
|
| + |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
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|
