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NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -


In our method, Cas9-gRNA is specifically expressed in germl cells to induce germline mutations.

For each gene, a transgenic line ubiquitously expressing a gRNA from the U6 promoter is established.

The U6-gRNA line is crossed to the nos-Cas9 line, which expresses Cas9 specifically in germ cells.

Progeny of the cross carry both the nos-Cas9 and U6-gRNA transgenes, which leads to formatin of an active Cas9-gRNA complex specifically in germ cells.

Male founder animals expressing Cas9-gRNA are crossed to wild-type females and their offspring are screend for an induced mutation by molecular genotyping.

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The NIG-Fly is a part of the National BioResource Project which started in 2002.