General
Information of NIG-FLY RNAi stocks
Q. What kind of fly stocks does NIG-FLY have?
Q. How
can I get a protocol for establishing fly stocks?
Q. How
can I get detailed information on a specific stock?
Q. Is
there any white-eyed fly contaminated?
Q. We
found Cy and Sb flies in some stocks.
Q. Are
there mites in your fly stocks?
Q. I
would like to know NIG-FLY recipe for Drosophila culture medium.
How to
use
Q. How
can I get RNAi fly stocks?
Q. I registered with DGRC, but I have not received my
DGRC ID.
Q. I
cannot log in with my DGRC ID.
Q. Do
I have to sign the MTA every time?
Q. What should I do before ordering transgenic flies?
Q. How
much dose it cost to receive RNAi fly stocks?
Q. When
will the charge be withdrawn from my account?
Q. Is
there any other way to pay except credit cards?
Q. The payment amount on the invoice has changed from the
first mail I received
Q. My
order has been canceled!
Q. Can
I order many stocks at a time?
Q. What
is your shipping schedule?
Q. I
cannot find the fly stocks I am looking for!
Q. I received a package without females/ larvae/ all are
dead.
Q. The
fly stocks I requested were not included in the shipment.
Q. Send
me some fly stock. I would like to culture them for sale.
Q. Send
me virgin flies. / Send me about 100 adult flies for my experiment.
How to use
Q. What kind of fly stocks does NIG-FLY have?
A. We provide RNAi fly stocks that can produce dsRNA by
crossing with an appropriate GAL4 driver and knockdown targeted genes (see “About
RNAi Fly”). You can download a list of full information from the top page
“Download All RNAi Stocks Files”.
Q. How can I get a protocol for establishing fly stocks?
A. Please see “About RNAi Fly--> Method-->
Injection and line establishment--> Fig.2”. Especially, we used w; Sp/SM1;
Pr/TM3 double balancer fly for establishing transgenic fly stocks.
Q. How can I get detailed information on a specific stock?
A. Search the stock ID, ex. 1001R-1, and click the “Stock
ID”. You will jump to “RNAi Stock Detail” which shows general stock
information, vector information, off-target information and so on.
Q. Is there any white-eyed fly contaminated?
A. Sorry but yes, there are possibilities of white-eyed
contamination. White+ is used as a marker but some lines are contaminated with
white-eyed flies. They are originated from a failure of balancing caused by
multiple insertions. We are trying to cure these lines, anyway please check
their eye color before using.
Q. We found Cy and Sb flies in some stocks.
A. We have established RNAi fly lines with SM1; TM3 balancer
chromosome. (w; Sp/SM1, Cy; Pr/TM3, Sb Ser, see About RNAi Fly-->
Method--> Injection and line establishment--> Fig.2). Generally our
stocks have been cleaned up for the balancers, but sometimes both balancers are
left when lethal mutation is caused on the non- P inserted chromosome
Q. Are there mites in your fly stocks?
A. We cannot guarantee that our stocks are mite free. Please
DO NOT mix with your clean stocks before inspection. However, we try keeping
our stocks under clean condition and frequent transfers in order to avoid
mites. If you find any problems in the stocks you received from us, please
contact “flyadmin AT nig DOT ac DOT jp”.
Q. I would like to know DGRC recipe for Drosophila medium.
A. Please see “About NIG-FLY”.
How to use NIG-FLY Stock Center
Q. How can I get RNAi fly stocks?
A. Please follow the procedure described below.
1) Get a DGRC ID by registering yourself from “Register
here!”.
If you agree to our policy, fill in the form and click
“submit”. The principal investigator, person responsible for payments, must
submit this form.
ID and password will be sent to you by email.
2) Search stocks by our searching form. You can use stock ID,
CG number, gene name and symbol(s) as keywords. You can add details using “Advanced
Search” and also upload or past multiple stocks copied from a list.
3) Click “Order” button and make sure your stock is added
into the cart. If you need more stocks, go back to the top page, search and
order again.
4) Click “Request” to order all stocks in your cart.
5) Read carefully instructions.
6) Log in by your DGRC ID and confirm your order. Select your
order type. If you do
NOT select the correct order type, your order will be cancelled even after the
completion of your order.
7) Read MTA and if you agree to our policy, check “agree” box.
If you select “Re-Order”, this process could be skipped.
8) Confirm your order again and proceed to payment.
9) Input your credit card information and complete the
status. You will receive a confirmation mail in a few minutes. If you don’t
receive this mail, contact us (flyadmin AT nig DOT ac DOT jp).
10) We will send you an e-mail with a tracking number when your order is ready for shipping.
Q. I registered with DGRC, but I have not received my DGRC ID.
A. Before you submit, make sure that your email address is
correct. DGRC ID and password will be sent automatically to the registered
email address. If you don’t receive the mail in few minutes, system might be down
or is in some kind of trouble. Please contact us (flyadmin AT nig DOT
ac DOT jp).
Q. I cannot log in with my DGRC ID.
A. Please make sure you have the right ID before you enter
our site. Four-digit number compose DGRC user ID, for example 1234.
“Drosophila Genetic Resource Center (DGRC) Kyoto,
Japan”, and “Drosophila Genomics Resource Center (DGRC) Bloomington, USA” are
completely different organizations. NIG-FLY and
Q. Do I have to sign the MTA every time?
A. Yes, we require the MTA for each new stock. Please contract
the MTA whenever you select “New Order”.
Q. What should I do before ordering transgenic flies?
A. Please complete all the necessary formalities at your
institute and your country. Transgenic flies at NIG-FLY are regulated under
Japanese laws and the Cartagena Protocol on Bio-safety of the Convension on
Biological Diversity. The regulation at the P1A level is required regarding
safe handling, storage, transport and use.
Q. How much dose it cost to receive RNAi fly stocks?
A. We charge 180 JPY per stock, 720 JPY per plasmid
, 2900 JPY per antibody(Culture Sup)
, 4330 JPY per antibody(Purified)
, 1230 JPY handling fee per
shipment and shipping fee. You can find the shipment fee to your destination here
.
Q. How should I pay?
A. We accept payment by credit card (
VISA or MasterCard only), which system is provided through a cryptographically protected
connection, SSL (Secure Socket Layer). To complete placing the order, you have
to fulfill your credit card information (such as card numbers and expiration
date), however this information will NOT be transmitted to NIG-FLY.
Pre-clearance period is from the day you place order until shipment is settled.
Actual clearance will be settled on the day we confirm your shipment.
Pre-clearance period should complete in
60 days
, otherwise your order will be
canceled.
Please see “Payment and Fee Information”.
Q. When will the charge be withdrawn from my account?
A. Clearance information will be sent to your credit card
company at the end of the month. Usually, you will be charged in two to three
months after the clearance date. If you have any questions, please contact your
credit card company.
Q. Is there any other way to pay except credit cards?
A. Sorry we only accept credit card.
Q. The payment amount on the invoice has changed from the first mail I received
A. We put rough fee information in the first confirmation
mail. During the shipment status, fee will be re-calculated after we check the
actual weight. Please check your final invoice attached to the shipment confirmation
mail.
Q. My order has been canceled!
A. There are few reasons for cancellation.
1) When you ask us to cancel the order.
2) When your credit card expire before the shipment. Please
make sure your card won’t expire at least in 90 days, during the pre-clearance
term.
Q. Can I order many stocks at a time?
A. 200 stocks a month per one lab/PI is the limit we can
accept.
Q. What is your shipping schedule?
A. NIG-FLY stock center ships fly stocks every Tuesday (once
a week). Orders, which MTA procedure has completed by Wednesday, will be
shipped next Tuesday. We will send a shipment confirmation mail with a tracking
number of the package as soon as we clarify the shipment.
We do not ship during Golden Week Holidays (early May),
Summer Bon Holidays (mid-August) and New Year Holidays. Please see “Shipping
Schedule”. Orders received after the deadline will be shipped after the
holidays.
Q. I cannot find the fly stocks I am looking for!
A. Please try using other keywords. You can also download
full information from the top page “Download All RNAi Stocks Files”. Sometimes
we hide weak stocks temporally from the list until they become available for
shipment. In this case, please wait a few weeks and search it again.
Q. I cannot place an order!
A.
1) Please see "How to order RNAi Stocks"
2) Check your OS & browser combination.
3) Erase all cookies and cashes left in you browser, both
from "shigen.lab.nig.ac.jp" and "www.shigen.nig.ac.jp".
Access directly to the following address and place an order again. http://www.shigen.nig.ac.jp/fly/nigfly/
If you still have problems, please let us know the details by email
(flyadmin AT nig DOT ac DOT jp).
Q. I received a package without females/ larvae/ all are dead.
A. We strictly check all of the fly stocks before dispatch.
Unfortunately, in case you find any stocks under those circumstances, please
let us know by stock ID(s), request ID, DGRC ID and name of PI as soon as
possible. We will send flies again for free. However, if the package was
confirmed to be delivered normally into your country, we cannot guarantee after
the condition, such as custom procedures. In some cases we should ask you to
place a new order.
Q. The fly stocks I requested were not included in the shipment.
A. Sometimes stocks are too weak or the demand for one
certain stock is too great for us to supply. The reason is printed on the invoice,
which is attached to the shipment confirmation mail. Stocks will be shipped as
soon as they become available for shipment with no additional fee. NIG-FLY
tries to delete weak stocks from our distribution list, however occasionally
the list might not been updated when you placed the order.
Q. Send me some flies. I would like to culture them for sale.
A. We will not send flies for the person/organization for the
purposes of profit-making business.
Q. Send me virgin flies. / Send me about 100 adult flies for my experiment.
A. Sorry, but NIG-FLY does not provide service of technical
assistance for your experiments. We will send enough flies for the next generation.
Please use the new born flies as you need.
Q. How can I express acknowledgements in papers?
A. Thank you very much. We suggest the following statement to be used: "The fly stock was obtained from NIG-Fly Stock Center." Please add an acknowledgement when publishing your research work using our RNAi fly stocks. Also, if you enter publication information to our “Reference Feedback“ system, your information will be added to our “RNAi stock detail” page. Many users will be able to access and share your paper information. Your cooperation would be very helpful for us to operate the center with continuous support from Japanese government.