Annual report of National Bioresource Project-Wheat II. DNA resources, 2007
Yasunari Ogihara1, Kanako Kawaura1, Hisako Imamura1, Keiichi Mochida2
1 Plant Genome Science Division, Kihara Institute for Biological Research, Yokohama City University, Maioka-cho Totsuka-ku, Yokohama 244-0813
2 RIKEN Plant Science Center, Suehiro-cho Tsurumi-ku, Yokohama 230-0045
Corresponding author: Yasunari Ogihihara
E-mail: yogihara@yokohama-cu.ac.jp
In the first year of the NBRP-Wheat II, DNA resource section is continuously promoting the systematic collection of cDNA clones and ESTs in addition to the stock of DNA resources collected in the first stage. The sequences of the full length cDNA clones were also collected supported by the NBRP Genome program in 2007. Achievements during April 2007 to March 2008 are reported here.
Collection of DNA resources: Total of ca. 85000 ESTs from four cDNA libraries were sequenced. These libraries were constructed from seedling of Norin 4 wheat under various conditions, namely seedlings treated with water (control), these infected with wheat blast fungus at 23oC, these incubated at 23oC after infection with oat blast fungus, and these incubated at 27oC after infection with oat blast fungus. Independent 5387 full length cDNA clones were selected and completed their sequencing. 14 TAC clones, including certain genes, were screened.
Maintenance of DNA resources: Approximately 40000 cDNA clones and 14 TAC clones were newly stored. EST information was processed to open at KOMUGI.
Distribution of DNA resources: 138 cDNA clones were distributed, of which 89 clones were sent to domestic researchers and 49 clones to foreign institutes. 14 TAC clones were sent to domestic researchers.
Related activity of DNA resources: The oligo-DNA microarray harboring 38k gene probes of common wheat were developed under the collaboration with Agilent Technology and distributed to domestic researchers. TILLING lines of Chinese Spring wheat were produced and all genomic DNA extracted from the TILLING lines were stored for use.