Fig. 2. RT-PCR analysis of differentially expressed gene transcripts after inoculation of wheat genotypes, Sonalika and Yangmai 6 with Bs culture. Transcript accumulation was analysed using total RNA extracted from leaves harvested at 0, 8, 24, 48 and 72 h after inoculation with Bs and mock-treated plants. RT-PCR products were detected in agrose gels stained with EtBr. Ubiquitin gene of barley was used to equalize the amount of cDNA for further analysis.