Fig. 1. Amplification of the PCR marker, pAWRC. 1, in the 1Rⁱ and 1R^Br dissection lines, and in the positive and negative control lines. The 1Rⁱ, 1RⁱL, 1RⁱS and 1R^Br lines had normal 1R chromosome of Imperial rye, its long arm, its short arm and normal 1R chromosome derived from a common wheat cultivar Burgas 2. For the structural characteristics of the dissection lines, see Table 1. Single asterisks indicate that the rearranged chromosomes are translocations, and the others are deletions.