Figure 1. Detection of ROS in seedling leaves of two wheat cultivars under low temperature condition. (A)(B) ROS detection by DCF-staining. M808 seedlings were grown for two weeks under the standard condition (A) and then treated with low temperature for 1 d (B). White bar = 100 µm. (C) ROS detection in mitochondria by DHR-staining of low temperature-treated seedlings of M808. White bar = 10µm. (D) ROS production by DAB infiltration assay in CS and M808. Two-week-old seedlings were treated with and without low temperature for 1 d. (E) Comparison of ROS production levels revealed by DCF-staining between CS and M808. The two-week-old seedlings were treated with low temperature for 1 d.