Figure 2. SDS-PAGE analysis of high-molecular-weight glutenin subunits. The Glu-A1, B1 and D1 alleles present in the four cultivars used in this work were determined by analysis of HMWG subunit patterns. Lane 1: 'Haruyutaka', 2: 'Takunekomugi', 3: 'Harunoakebono', 4: 'Norin 61'.