Figure 2.  Phosphorylation of WCOR15 and WCOR14.  (A) in vitro phosphorylation assay.  C, negative control; NA, non-acclimated; A5, 5-d cold-acclimated; 14, WCOR14; 15, WCOR15.  (B) In-gel phosphorylation assay.  Protein extracts from M808 seedling leaves were separated on SDS-PAGE gels containing 0.1 mg/ml of recombinant WCOR15 or WCOR14.  NA, non-acclimated; A1 to A10, 1-d to 10-d cold-acclimated; L, 16h light/8h dark; D, continuous dark.