Strain number Strain Name Sex Plasmid integrated Plasmid autonomous Prophage Genotype Parent donor Parent recipient Methods Latest marker Selection Source Culture condition Other remarks Reference (RRC ID) Reference (exclude RRC) ME8166 YK4101 F- araD139 pyrC46 gyrA thi thyA rpsL DE((lac)U169) flaA371 YK410 TD./transduction Y. Komeda 30106 ME8167 YK4102 F- thi gyrA flaB349 pyrC46 araD139 rpsL DE((lac)U169) thyA YK410 F'-duction Y. Komeda 30106 ME8168 YK4103 F- thi flaC101 araD139 DE((lac)U169) rpsL pyrC46 gyrA thyA YK410 F'-duction Y. Komeda 30106 ME8169 YK4104 F- flaD867 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 F'-duction Y. Komeda 30106 ME8170 YK4105 F- flaE694 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 TD./transduction Y. Komeda 30106 ME8171 YK4106 F- hag-726 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 F'-duction Y. Komeda 30106 ME8172 YK4111 F- flaN1861 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 TD./transduction Y. Komeda 30106 ME8173 YK4112 F- flaO1862 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 TD./transduction Y. Komeda 30106 ME8174 YK4113 F- flaP871 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 F'-duction Y. Komeda 30106 ME8175 YK4114 F- flaQ1083 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 F'-duction Y. Komeda 30106 ME8176 YK4115 F- DE((lac)U169) flaR1004 araD139 rpsL thi pyrC46 gyrA thyA YK410 F'-duction Y. Komeda 30106 ME8177 YK4116 F- flb-4116 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA his YK410 Y. Komeda 30106 ME8178 YK4117 F- motD4117 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA his YK410 Y. Komeda 30106 ME8179 YK4118 F- flaL4118(amber) araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA his YK410 Y. Komeda 30106 ME8180 YK4119 F- motA4119 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA his YK410 Y. Komeda 30106 ME8181 YK4120 F- flaQ4120 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8182 YK4121 F- flaH4121 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8183 YK4122 F- flaU4122 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8184 YK4123 F- motA4123 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8185 YK4124 F- motA4124 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8186 YK4125 F- gyrA flaB4125 araD139 DE((lac)U169) rpsL thi pyrC46 thyA YK410 Y. Komeda 30106 ME8187 YK4126 F- flaB4126 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8188 YK4127 F- flaC4127 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8189 YK4128 F- flbB4128 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8190 YK4129 F- flbB4129 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8191 YK4130 F- hag-4130 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8192 YK4131 F- flbB4131 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8193 YK4132 F- flaL-M4132 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8194 YK4133 F- flaH4133? araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8195 YK4134 F- flaBC4134 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8196 YK4135 F- flaBC4135 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8197 YK4136 F- flaI4136 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8198 YK4137 F- flaI4137 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8199 YK4138 F- flaBC4138 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8200 YK4139 F- flbC4139 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8201 YK4142 F- flaBC4142 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8202 YK4143 F- flaL-M4143 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8203 YK4144 F- flaP4144 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8204 YK4145 F- flaN4145 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8205 YK4146 F- hag-4146 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8206 YK4147 F- flaC4147 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8207 YK4148 F- flaM4148 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8208 YK4149 F- gyrA flaPQR4149 araD139 DE((lac)U169) rpsL thi pyrC46 thyA YK410 Y. Komeda 30106 ME8209 YK4150 F- flaR4150 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8210 YK4151 F- flaK4151(amber) araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8211 YK4152 F- hag-4152 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8212 YK4153 F- flaC4153 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8213 YK4154 F- flaP4154 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8214 YK4155 F- flaI4155 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8215 YK4156 F- flaL-M4156 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8216 YK4157 F- flaH4157 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8217 YK4158 F- flbC4158 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8218 YK4159 F- flbC4159 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8219 YK4160 F- flaA4160 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8220 YK4161 F- hag-4161 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8221 YK4162 F- flaI4162 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8222 YK4163 F- hag-4163 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8223 YK4164 F- flaY4164 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8224 YK4165 F- flaB4165 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8225 YK4166 F- flaB4166 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8226 YK4167 F- flaB4167 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8227 YK4168 F- flaA-R araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda flaA-R(bush:hard to tell) 30106 ME8228 YK4169 F- flaL-M4169 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8229 YK4170 F- flaR4170 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8230 YK4171 F- flaH4171 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8231 YK4172 F- flaH4172 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8232 YK4173 F- fla?4173 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8233 YK4174 F- motA4174 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8234 YK4175 F- flaI4175 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8235 YK4176 F- flaT4176 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8236 YK4177 F- flaS-T4177 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8237 YK4178 F- flaC4178 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8238 YK4179 F- motA4179 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8239 YK4180 F- flaQ-P? araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda flaQ-P?(hard to tell) 30106 ME8240 YK4181 F- flaD4181 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8241 YK4182 F- fla?4182 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda fla?4182(hard to tell) 30106 ME8242 YK4183 F- motB4183 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8243 YK4184 F- flaI4184 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8244 YK4185 F- flaI?4185 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8245 YK4186 F- flaU4186 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8246 YK4187 F- flaI4187 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8247 YK4188 F- flaI4188 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8248 YK4189 F- flaB4189 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8249 YK4190 F- flaC4190 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8250 YK4191 F- flaB4191 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8251 YK4192 F- hag-4192 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8252 YK4193 F- rpsL flaN4193 araD139 DE((lac)U169) thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8253 YK4194 F- flaI?4194 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8254 YK4195 F- flaG4195 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8255 YK4196 F- flaH4196 araD139 DE((lac)U169) rpsL thi pyrC gyrA thyA YK410 Y. Komeda 30106 ME8256 YK4197 F- flaH4197 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8257 YK4198 F- flaH4198 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8258 YK4199 F- flaH4199 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8259 YK4200 F- flaH4200 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA YK410 Y. Komeda 30106 ME8574 YK4516 F- hag::Tn10 araD139 DE((lac)U169) rpsL thi pyrC46 gyrA thyA P1S YK5016 YK410 hag::Tn10 TcR Y. Komeda 29867 ME5302 PC33 Lac- F- thi thyA str lac dnaG3 PC33/F lac SPONTANEOUS (F lac)- Lac- on EMB ME5303 PC33SSm Thy+#1 F- thi lac dnaG3 G6 PC33 Lac-(ME5302) CROSS str+ Thy+ His+ 35664 ME5304 HfrT266 Hfr F HfrP4X8(proB -> lac) thi arg dnaB266 his str P4X8 CRT26617 CROSS dnaB266, Hfr Lac+ Met+ ME5310 HfrT42Thy- Hfr F HfrP13(pyrE <- dnaA) his thy mel mtl dnaB42 HfrT42 SPONTANEOUS thy- TrimetoprimeR ME5312 HfrPC33 Hfr F HfrP4X8(proB -> lac) thy str dnaG3 P4X8 PC33 Lac-(ME5302) CROSS dnaG3 Lac+ Met+ StrR ME5314 KT129 F- trp(am) tyr(am) thy thr his sup-126 polA12 dapE9 ptsI7 T. Nagata ME5320 KS268 F- thyA lacY-A482 lig(ts7) str R. Okazaki ME5321 HfrKS268 Hfr F HfrP4X8(proB -> lac) thyA lig(ts7) str P4X8 KS268(ME5302) CROSS Hfr Lac+ StrR ME5325 H677 F- his-68 tyrA2 purC50 tonA2 lacY1 tsx-70 supE44? gal-6 trp-45 rpsL125 malT1 lambda_R xyl-7 mtl-2 thi-1 M. Abe Note by M. Yamada, see back side 29998, 29936 ME5327 DF44zwf27 Hfr F HfrH(pyrB <- pil) pgi zwf Y. Komeda 29938, 29901, 29879 ME5330 PC35 Hfr F HfrC(purE -> lip) met dnaG3 HfrPC3 LC102(ME7769) CROSS dnaG3 ArgG+ Thy+ ME5331 PC33 Spc F- leu thy str lac spc dnaG3 CRT266spc PC33 spc SpcR Y. Hirota ME5332 PC34SS Spc F- leu his purE spc lac dnaG3 E5014(ME8617) PC34 str+ SpcR ME5333 S1814 F+ F sup endA str thyA dnaH1814 T. Komano ME5334 S1814Thy+ F+ F endA sup str dnaH18 W3110 S1814 thy+ Thy+ ME5335 S1814spc F+ F endA sup spc thyA dnaH1814 E5014 S1814 strS SpcR ME5337 CRT4624(KLF11+) F+ F thi thr leu his arg lacY xyl malA mtl ara galB str tonA dnaA46 LC102recA/KLF11 CRT4624 CROSS KLF11+ Arg+ Pro+ ME5338 CRT4624Flac+ F+ F thi thr leu his arg lacY xyl malA mtl ara galB str tonA dnaA46 LC169 CRT4624 CROSS Flac+ Lac+ StrR ME5339 CRT4624F8+ F+ F thi thr leu his arg lacY xyl malA mtl ara galB str tonA dnaA46 JE407 CRT4624 CROSS F8+ Gal+ StrR ME5340 CRT4624R100-1+ F- Other thi thr leu his arg lacY xyl malA mtl ara galB str tonA dnaA46 JE1916 CRT4624 CROSS R100-1+ CMR Met+ ME5341 LC343F15+ F+ F leu thyA DE(proB-lac) dnaA46 JE905 LC343 CROSS F15+ Thy+ Met+ ME5342 LC343F8+ F+ F leu thyA DE(proB-lac) dnaA46 LC102thy-(F8+) LC343 CROSS F8+ Pro+ PurE+ Arg+ Met+ ME5343 LC343F9+ F+ F leu thyA DE(proB-lac) dnaA46 JE3359 LC343 CROSS F9+ Met+ ME5344 LC343Sm F- leu thyA DE(proB-lac) str dnaA46 LC102thy- LC343 str StrR ME5347 PC5 F- leu thyA str dnaA5 C. Savastopoulos 29978 ME5350 AB1255 F- thi-1 ilvA201 argH1 metB1 his-1 xyl-7 malA1 mtl-2 ara-13 lacY1 or Z4 gal-6 str tonA2 tsx5 supE44 B. Bachmann ME5351 AB2277 F- ilvD145 metE46 his-4 trp-3 pro-2 thi-1,2 or 12 mtl-1 mal-1 ara-9 gal-2 lac-114 ton-1 str-8 or 9 Y. Hirota ME5352 AB2826SR F- aroB351 str F. Gibson 30003 ME5354 AB3292 F- pabA his-4 pro-2 arg-3 ilv-7 str F. Gibson ME5355 AN92 F- thi pro arg trp tyr phe aroB F. Gibson Require shikimic acid(10_micro_M) in minimal medium ME5356 AN193 F- entA403 thi-1 leu-6 proC14 trpE48 mtl-1 xyl-5 lacY1 ara-14 str-109 azi-6 tonA23 tsx-67 supE44 B. Bachmann 29983 ME5357 AT713 F- thi-1 argA21 cysC43 lysA22 mtl-2 xyl-7 malA1 str-104 supE44 B. Bachmann ME5358 AT718 F- argA21 cysG44 his-1 malA1 mal-18(?) xyl-7 mtl-2 str-104 supE44 B. Bachmann ME5360 AT2255 F- leu tonA lac gal his str malA xyl mtl argG6 metB1 thyA1 deo D. A. Glaser ME5361 AT2279 F- met pro mtl mal str ara his trp gal-2 lac ilvD88 ton tsx thi C. Savastopoulos ME5363 AT2538 F- thi-1 pyrE60 argE3 his-4 proA2 thr-1 leu-6 mtl-1 xyl-5 ara-14 galK2 lacY1 strA31 supE44 B. Bachmann ME5364 AT2699 F- argG6 metC his-1 thyA3 malA1 gal-6 lacY1 or Z4 mtl-2 str tsx-64 supE44 B. Bachmann ME5365 AT3143 F- pyrF30 ilv-277 met-65 his-53 purE41 proC24 pdxC3 xyl-14 lacY29 str-97 cycA1 cyc-2 tonA2 tsx-63 B. Bachmann ME5366 AX729 lac thi str A. Taketo ME5367 CR34T2 F- thi thr leu thyA deoC CR34 SPONTANEOUS S. Yasuda ME5368 CSH54 F- DE(proB-lac) supF trp pyrF his thi str T. Icho ME5369 CU59 F- proC ilvF405 H. E. Umbarger 29915 ME5370 CU309, UTH4067 F- his tyrA trp purC guaA H. E. Umbarger 29915 ME5371 CU310 F- nadB3 H. E. Umbarger 29915 ME5373 DCSP4 F- dapD his met ilv str trp mtl xyl AT980 LC102Thy- dapD Pro+ StrR trp is leaky. ME5374 DCSP6 F- trp leu argG ilv his met dapD purE str mtl xyl AT980 LC102thy- CROSS dapD Pro+ StrR ME5375 DCSP7 F- argG thy ilv his dapD purE str mtl xyl AT980 LC102thy- dapD Pro+ StrR ME5376 DCSP17 F- purE ilv argG leu his uvrA str HfrT42TR2 PC0021(ME5481) CROSS uvrA Met+ StrR ME5377 DCSP22 F- purE argG leu his uvrA str HfrT42TR2 DCSP6(ME5374) CROSS uvrA Met+ StrR ME5383 E726,H724 F- his tyrA trp purC guaB thi gal lac str B. Egan 29880 ME5385 EM1003 F- aroC purF his trp str ara gal lac mal xyl E. Mcfall 30012, 29776 ME5386 EM1005 F- dsdA1 purE his trp E. Mcfall 29776 ME5387 EM3003 F- aroC purC lac thi str dsdA7 mal E. Mcfall 30012 ME5390 FF8018 F- proC str ptsI W. Epstein -> T. Nagata 29972 ME5393 JC2915 F- thr-1 leu-6 thi-1 supE44 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 str-31 tsx-33 sup-37 cysC43 R. Lloyd 29765 ME5395 JM70 F- thr leu arg his trp cysE R. J. Kadner 29883 ME5396 JP1430 F- pyrB59 thi-1 his-1 purC1 xylA1 malA1 valS351 str-48 A. F. Egan ts growth ME5397 JP5042 F- cycA352 purA rif-356 str-724 A. F. Egan supplement 40_micro_g/ml Ade 29896 ME5398 KL231 F- leuS31 thyA6 deoC1 strA120 B. Bachmann ts growth 29988 ME5401 KL399 F- thi-1 metE70 leu-6 proC32 malA38 hisF860 thyA54 lacZ36 ara-14 mtl-1 xyl-5 str-109 spc-15 recA200 R. Lloyd recA200-ts recA 29927 ME5402 KY5054 F- thr leu metB thi lac his gal malA xyl mtl ara tonA str azi stv-54 T. Yura ts-growth streptovalicin and rifampicin resistant ME5403 LC102Thy F- proC purE argG leu trp thi ile his metB ara lacY gal xyl str tsx thy LC102(JE5597) SPONTANEOUS thy Trimethoprim Y. Nishimura ME5404 LC102PolA12#1 F- thi leu argG ilv his trp purE proC str lacY gal xyl mtl polA12 CROSS polA12 Met+ StrR ME5405 LD195 F- Other PI Lysogen nrdB cdd dcd his argG metB leu lacY or Z malA xyl mtl gal tpp(=deoA) str J. A. Fuchs 29774 ME5406 MI1746 F- brnQ2 thi metE purE trp lysA ara xyl str tonA tsx M. Iaccarino 29920 ME5407 MI183a F- ilvH611 thi-1 metE proC purE trp lysA ara xyl lacZ azi str tonA tsx M. Iaccarino 29929 ME5409 MP1 F- uxuB8 thr leu pro thyA arg(H) str J. Robert-Baudouy 29923, 29921 ME5410 N43 F- acrA1 lac str gal ara H. Nakamura 29783 ME5411 N1235 F- thi thr leu lacY mtl xyl ara galK his proA argE tsx supE37 str J. L. Rosner 29894 ME5412 N1310 F- Other P1-Clr100 thi thr leu lacY mtl xyl ara galK his proA argE tsx supE37 str J. L. Rosner 29894 ME5413 N2047 F- metB1 his rnc-105 ranA2074 D. Apirion 29784 ME5414 N2074 F- thi-1 argH1 nadB4 lacY1 gal-6 malA1 xyl-7 ara-13 mtl-2 str-9 tonA2 supE44 ranA2074 D. Apirion 29784 ME5415 N2077 F- lacY1 thi-1 argH1 nadB4 gal-6 malA1 xyl-7 ara-13 mtl-2 str-9 tonA2 supE44 rnc-105 D. Apirion 29784 ME5418 PB3 F- his tolC uxaC str J. Robert-Baudouy ts-growth 30045, 29923 ME5419 PBT1 F- his thyA metC uxaC1 argG str J. Robert-Baudouy KmR provably rec- (UVS, unable to P1.transduction) 29923 ME5421 PC0172, H1029 F- thi his tyrA trp guaB lacY gal xyl mal tonA tsx str Phabagen Collection ME5422 PC0207, H1173 F- thi his tyrA trp proA purH guaC lacY gal xyl mtl mal tonA tsx str phx Phabagen Collection ME5423 PC0291, H1233 F- leu proA pdxA pyrA lacY gal xyl ara mtl mal tsx str Phabagen Collection ME5424 PC0361, KMBL49 thr leu thi cdd pyrF thyA lacY tonA deo supE Phabagen Collection P. G. de Hann ME5425 PC0700, H888 F- thi his trp purG lacY gal xyl mtl mal tonA str Phabagen Collection ME5427 PC1528, AT2681 F- argH his purF glyA thi lac xyl mtl mal str Phabagen Collection ME5428 PL8-31 F- thr-1 leu-6 thi-1 proA2 hisC3 metG87 metK86 serA25 glc-1 lacY1 galK2 mtl-1 xyl-5 ara-14 str-25 (lambda)- supE44(?) Y. Komeda ME5430 RE1 F- proA trp his lacY strA E. C. R. Reeve ME5431 RE26 F- proA trp his lacY1 tsx E. C. R. Reeve ME5432 RE315 F- proA trp his lacY tsx strB E. C. R. Reeve strB : 5_gamma_SmR ME5433 RE103 F- proA23 trp-30 his-51 lac-28 cmlA1 rpsL101 E. C. R. Reeve -> B. Bachmann -> M. Ohta CM : 6_micro_g/ml : + 10_micro_g/ml : - 29995, 29949, 29940 ME5434 RK1032 F- thr leu argE3 proA2 his pyrE60 thi R. J. Kadner 29883 ME5435 S343Spc#3 F- aroD argEH X-? man gal lac mtl str spc E5014 S343 spc SpcR Y. Nishimura ME5436 S491 F- his proA galT22 lac uraP tonA R. Lloyd 29765 ME5437 SH10 F- ilv trpE tonB tdk-1 tsx S. Hiraga -> S. Yasuda ME5438 S_phi_200 F- metB purB str DE(add-uid-man) P. Nygaard 29763 ME5439 S_phi_333 F- metB purB str add P. Nygaard 29763 ME5440 S_phi_609 F- ara thi hpt pup(=deoD) purH or J str DE(pro-gpt-lac) P. Nygaard -> Y. Takeda 30014, 29763 ME5441 SP10 F- arg met thi his xyl mtl mal str bglA bglB bglR bglS S. Schaefler 29932 ME5442 SP33 F- bglR bglD S. Schaefler 29932 ME5443 TK405m F- lacZ gal rha malA kdpABC5 trkA trkD W. Epstein require 115mM K+ 66250, 29987 ME5444 TolVIII F- tolC K. Onodera chromosomal markers are unknown without tolC ME5445 TrpS F- trpS10330 M. J. Marinus ts-growth ME5447 W3110His-SmR F- his str T. Icho ME5450 Wsuc17 F- sucB9 (lambda)- B. Bachmann 29969 ME5452 Y73 F- F female3 lac-1103 mal-5 lys leu str H. Uchida -> Y. Nishimura tight Lys-, female3(W3876) ME5454 AN144 Hfr F HfrP13(pyrE <- dnaA) metB ubiA420 F. Gibson 29891 ME5456 AT997 Hfr F HfrKL16(lysA -> serA) thi-1 rel-1 dapC15 B. Bachmann ME5457 AT980 Hfr F HfrKL16(lysA -> serA) thi-1 rel-1 dapD2 B. Bachmann supplement 50_micro_g/ml DAP ME5458 AT982 Hfr F HfrKL16(lysA -> serA) thi-1 dapD4 rel-1 B. Bachmann supplement 50_micro_g/ml DAP 29980 ME5459 AT1243 Hfr F HfrH(valS <- uxuAB) pyrE metE Y. Hirota no fertility 30178 ME5461 AT2455 Hfr F HfrH(pyrB <- thr) thi-1 cysG44 mal-18 rel-1 B. Bachmann ME5462 AT2459 Hfr F HfrH(pyrB <- thr) thi-1 serB22 rel-1 B. Bachmann ME5463 AT2472 Hfr F HfrH(pyrB <- thr) thi-1 rel-1 aroE24 B. Bachmann ME5467 CM8 Hfr F HfrP4X(proB -> lac) uxu8 metB1 J. Robert-Baudouy 29923 ME5471 E771,KL164 Hfr F HfrKL16(lysA -> serA) thi-1 thyA24 nalB14 rel-1 B. Egan 29961 ME5472 ES430 Hfr F HfrH(pyrB <- thr) thi-1 rel-1 malB29 B. Bachmann Mal+ (by M. Yamada) ME5474 HfrCU482 Hfr F HfrH(pyrB <- thr) asd M. Schwatz 29957 ME5475 HfrAB1886 Hfr F HfrP4X8(proB -> lac) his arg str uvrA HfrP4X8 AB1886 CROSS Hfr Lac+ Met+ ME5476 JC182-9 Hfr F Hfr(thyA <- argG pyrB <- thr) thi argR purC W. Maas double male 29985 ME5478 KS5 Hfr F HfrH(pyrB <- thr) DE(gal-uvrB) Y. Komeda 29889 ME5479 MH1 Hfr F HfrP4X(proB -> lac) uxaC1 metB1 J. Robert-Baudouy 35648, 29923, 29921, 4924 ME5480 MI247 Hfr F HfrH(pyrB <- thr) brnR6(Am) thi his lac(Am) M. Iaccarino 29920 ME5481 PC0021 Hfr F HfrP4X8(proB -> lac) metB pyrA purH argF P. G. de Haan 29998 ME5484 PK191 Hfr F HfrPK191(cheC <- supD) thi DE(proB-lac) sup56 B. Bachmann ME5485 Pr11purE+ Hfr F HfrP4X8(proB -> lac) leu metB ile thi argG xyl mtl str his trp gal AB1157 Pr11 purE+ PurE+ ME5486 RE82 Hfr F Hfr(lac <- gal) metB E. C. R. Reeve ME5488 1278RecA F- thr leu arg his trp str lac malA xyl mtl tonA dnaB1278 recA1 LC248 JE11278 CROSS recA1 Thy+ StrR ME8781 CAG18442 F- thr-34::Tn10 NK5148 MG1655 thr-34::Tn10 TcR M. Singer requires Thr
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8782 CAG12093 F- car-96::Tn10 NK6034 MG1655 car-96::Tn10 TcR M. Singer requires uracil and Arg
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8783 CAG12095 F- zac-3051::Tn10 FMJ201 MG1655 zac-3051::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8784 CAG12025 F- zad-220::Tn10 panD SJ16 MG1655 zad-220::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8785 CAG18436 F- zae-502::Tn10 JW353 MG1655 zae-502::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803, 861 ME8786 CAG18447 F- proAB81(proAB::Tn10) CAG1681 MG1655 proAB81::Tn10 TcR M. Singer requires Pro
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8787 CAG12080 F- zah-281::Tn10 RS1071 MG1655 zah-281::Tn10 TcR M. Singer from CAG12049
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8788 CAG18439 F- lacI42::Tn10 lacZ(U118) CAG1538 MG1655 lacI42::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 35386, 35355, 35443, 29803, 4951, 3283 ME8790 CAG12148 F- tsx-247::Tn10 P2719 MG1655 tsx-247::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8791 CAG12017 F- zba-3054::Tn10 SG20253 MG1655 zba-3054::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8792 CAG12154 F- zbb-3055::Tn10 M145 MG1655 zbb-3055::Tn10 TcR M. Singer Co-td purE/zeb-1::Tn10 : 8%
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8793 CAG12171 F- purE79::Tn10 NK6051 MG1655 purE79::Tn10 TcR M. Singer requires purines
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8794 CAG12021 F- zbc-3105::Tn10 MG1655 zbc-3105::Tn10 TcR M. Singer Co-td purE/zbc-3105::Tn10 : 4%
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8795 CAG12149 F- zbd-601::Tn10 RK4342 MG1655 zbd-601::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8796 CAG12077 F- zbe-280::Tn10 SK2257 MG1655 zbe-280::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803, 4985 ME8797 CAG18433 F- zbf-3057::Tn10 P2217 MG1655 zbf-3057::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8799 CAG18493 F- zbh-29::Tn10 CAG18392 MG1655 zbh-29::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8801 CAG18478 F- zbj-1230::Tn10 RW1230 MG1655 zbj-1230::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8802 CAG12094 F- zcb-3059::Tn10 DC304 MG1655 zcb-3059::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8803 CAG18466 F- zcc-282::Tn10 DC305 MG1655 zcc-282::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8804 CAG12078 F- zce-726::Tn10 TL212 MG1655 zce-726::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8805 CAG18463 F- zcf-117::Tn10 RS3242 MG1655 zcf-117::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8806 CAG18497 F- fadR13::Tn10 RS3040 MG1655 fadR13::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8807 CAG12016 F- zcg-3060::Tn10 ORN125 MG1655 zcg-3060::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8809 CAG12169 F- zch-506::Tn10 JW380 MG1655 zch-506::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8810 CAG12028 F- zci-233::Tn10 PK1085 MG1655 zci-233::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8811 CAG12081 F- zcj-3061::Tn10 EC2111 MG1655 zcj-3061::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8812 CAG12026 F- trg-2::Tn10 FMJ200 MG1655 trg-2::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8813 CAG18461 F- zcd-235::Tn10 PLK1269 MG1655 zcd-235::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8814 CAG18459 F- zde-234::Tn10 PLK1253 MG1655 zde-234::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8815 CAG18462 F- zdg-603::Tn10 UT152 MG1655 zdg-603::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8816 CAG12151 F- zdh-925::Tn10 DF949 MG1655 zdh-925::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8817 CAG18464 F- zdi-276::Tn10 BJW72 MG1655 zdi-276::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8818 CAG18465 F- zdj-225::Tn10 DC369 MG1655 zdj-225::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8819 CAG12074 F- zea-3068::Tn10 DC374 MG1655 zea-3068::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8820 CAG18486 F- eda-51::Tn10 N3041 MG1655 eda-51::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8821 CAG12068 F- zeb-3190::Tn10 MG1655 zeb-3190::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8822 CAG12156 F- uvrC279::Tn10 N3024 MG1655 uvrC279::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8823 CAG18451 F- zed-3069::Tn10 MG1655 zed-3069::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8824 CAG12099 F- zee-3129::Tn10 DC411 MG1655 zee-3129::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8825 CAG12179 F- mgl-500::Tn10 LA5606 MG1655 mgl-500::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8826 CAG12098 F- zeg-722::Tn10 LA5651 MG1655 zeg-722::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8827 CAG12177 F- zeh-298::Tn10 CS1230 MG1655 zeh-298::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8828 CAG12178 F- zei-723::Tn10 DL2 MG1655 zei-723::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8829 CAG18484 F- zej-223::Tn10 DC334 MG1655 zej-223::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8831 CAG18467 F- zfb-1::Tn10 RS3116 MG1655 zfb-1::Tn10 TcR M. Singer formerly zfa-1::Tn10
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8832 CAG18468 F- nupC510::Tn10 S_phi_1024 MG1655 nupC510::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8833 CAG18470 F- purC80::Tn10 NK6056 MG1655 purC80::Tn10 TcR M. Singer requires purines
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8835 CAG18481 F- zff-208::Tn10 BW280 MG1655 zff-208::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8838 CAG18642 F- zfh-3131::Tn10 MG1655 zfh-3131::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8839 CAG12173 F- cysC95::Tn10 N3002 MG1655 cysC95::Tn10 TcR M. Singer requires cys
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8840 CAG12079 F- fuc-3072::Tn10 JK1015 MG1655 fuc-3072::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8841 CAG12135 F- recD1901::Tn10 MG1655 recD1901::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8842 CAG18709 F- zgc-3074::Tn10 DC367 MG1655 zgc-3074::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8843 CAG12168 F- zgd-210::Tn10 DF264 MG1655 zgd-210::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8844 CAG18472 F- nupG511::Tn10 S_phi_1023 MG1655 nupG511::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8845 CAG18475 F- metC162::Tn10 NK6027 MG1655 metC162::Tn10 TcR M. Singer requires met
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 35355, 29803 ME8846 CAG12184 F- tolC210::Tn10 P2727 MG1655 tolC210::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 35436, 29803 ME8847 CAG12152 F- zgh-3075::Tn10 CAG18164 MG1655 zgh-3075::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8848 CAG12072 F- zgj-203::Tn10 SK2262 MG1655 zgj-203::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803, 4985 ME8849 CAG12153 F- zha-6::Tn10 DV6 MG1655 zha-6::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8850 CAG12071 F- zhb-3082::Tn10 JW375 MG1655 zhb-3082::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8851 CAG12159 F- zhc-9::Tn10 DV9 MG1655 zhc-9::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8854 CAG18452 F- zhe-3085::Tn10 CAG8007 MG1655 zhe-3085::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8855 CAG18450 F- zhf-50::Tn10 CAG2228 MG1655 zhf-50::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8856 CAG18638 F- zhg-3086::Tn10 MG1655 zhg-3086::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8857 CAG18640 F- zhj-3076::Tn10 MG1655 zhj-3076::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8858 CAG12163 F- zib-207::Tn10 BW322 MG1655 zib-207::Tn10 TcR M. Singer formerly zia-207::Tn10
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8859 CAG18492 F- zic-4901::Tn10 RK4901 MG1655 zic-4901::Tn10 TcR M. Singer formerly zib-4901::Tn10
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8860 CAG18499 F- zid-501::Tn10 JW355 MG1655 zid-501::Tn10 TcR M. Singer formerly zic-501::Tn10
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8861 CAG18501 F- zie-296::Tn10 SK2210 MG1655 zie-296::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8862 CAG18431 F- ilv-500::Tn10 MBGO MG1655 ilv-500::Tn10 TcR M. Singer requires ile and val
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8864 CAG18496 F- fadAB101::Tn10 RS3087 MG1655 fadAB101::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8865 CAG18495 F- zih-35::Tn10 SK1861 MG1655 zih-35::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8867 CAG12185 F- argE86::Tn10 CAG8396 MG1655 argE86::Tn10 TcR M. Singer requires Arg
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 35355, 29803 ME8869 CAG18498 F- zjb-504::Tn10 RS162 MG1655 zjb-504::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8870 CAG12164 F- malF3089::Tn10 TST3 MG1655 malF3089::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8871 CAG18488 F- zjd-2231::Tn10 AKK231 MG1655 zjd-2231::Tn10 TcR M. Singer Co-td melB/zjd-2231::Tn10 : 15% ampC/zjd-2231::Tn10 : 50%
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8872 CAG18427 F- zje-2241::Tn10 AKK241 MG1655 zje-2241::Tn10 TcR M. Singer Co-td melB/zje-2241::Tn10 : 81% ampC/zje-2241::Tn10 : 66%
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8873 CAG12073 F- cycA30::Tn10 GR401 MG1655 cycA30::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8874 CAG12019 F- zjh-920::Tn10 DF1062 MG1655 zjh-920::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8875 CAG18429 F- zji-6::Tn10 SK597 MG1655 zji-6::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8876 CAG18430 F- zjj-202::Tn10 SK472 MG1655 zjj-202::Tn10 TcR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8877 CAG18425 F- thr-3091::Tn10kan CAG18442(ME8781) OTHER METHODS (Tn10kan conversion) thr-3091::Tn10kan KmR M. Singer requires Thr
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8878 CAG18620 car-3092::Tn10kan CAG12093(ME8782) OTHER METHODS (Tn10kan conversion) car-3092::Tn10kan KmR M. Singer requires uracil and Arg
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8879 CAG12131 zac-3093::Tn10kan CAG12095(ME8783) OTHER METHODS (Tn10kan conversion) zac-3093::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8880 CAG12105 zad-3094::Tn10kan CAG12025(ME8784) OTHER METHODS (Tn10kan conversion) zad-3094::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8881 CAG18580 zae-3095::Tn10kan MG1655 OTHER METHODS (Tn10kan conversion) zae-3095::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8882 CAG18515 proAB3096::Tn10kan CAG18447(ME8786) OTHER METHODS (Tn10kan conversion) proAB3096::Tn10kan KmR M. Singer requires Pro
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8883 CAG18633 zag-3198::Tn10kan MG1655 OTHER METHODS (Tn10kan conversion) zag-3198::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8884 CAG18420 lacI3098::Tn10kan lacZ(U118) CAG18439(ME8788) OTHER METHODS (Tn10kan conversion) lacI3098::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 35355, 29803 ME8886 CAG18413 tsx-3100::Tn10kan CAG12148(ME8790) OTHER METHODS (Tn10kan conversion) tsx-3100::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8887 CAG12107 zba-3101::Tn10kan CAG12017(ME8791) OTHER METHODS (Tn10kan conversion) zba-3101::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8889 CAG12116 zbc-3200::Tn10kan CAG12021(ME8794) OTHER METHODS (Tn10kan conversion) zbc-3200::Tn10kan KmR M. Singer Co-td purE/zbc-3200::Tn10kan : 4%
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8891 CAG12123 zbe-3105::Tn10kan CAG12077(ME8796) OTHER METHODS (Tn10kan conversion) zbe-3105::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8892 CAG18514 zbf-3106::Tn10kan CAG18433(ME8797) OTHER METHODS (Tn10kan conversion) zbf-3106::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8893 CAG18341 nadA3052::Tn10kan CAG12147(ME8798) OTHER METHODS (Tn10kan conversion) nadA3052::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8894 CAG18531 zbh-3108::Tn10kan CAG18493(ME8799) OTHER METHODS (Tn10kan conversion) zbh-3108::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8896 CAG18528 zbj-3110::Tn10kan CAG18478(ME8801) OTHER METHODS (Tn10kan conversion) zbj-3110::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8897 CAG12130 zcb-3111::Tn10kan CAG12094(ME8802) OTHER METHODS (Tn10kan conversion) zcb-3111::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8898 CAG18613 zcc-3112::Tn10kan CAG18466(ME8803) OTHER METHODS (Tn10kan conversion) zcc-3112::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8899 CAG12124 zce-3113::Tn10kan CAG12078(ME8804) OTHER METHODS (Tn10kan conversion) zce-3113::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8900 CAG18516 zcf-1314::Tn10kan CAG18463(ME8805) OTHER METHODS (Tn10kan conversion) zcf-1314::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8901 CAG18544 fadR3115::Tn10kan CAG18497(ME8806) OTHER METHODS (Tn10kan conversion) fadR3115::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8902 CAG12106 zcg-3116::Tn10kan CAG12016(ME8807) OTHER METHODS (Tn10kan conversion) zcg-3116::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8903 CAG18551 zch-3117::Tn10kan CAG12169(ME8809) OTHER METHODS (Tn10kan conversion) zch-3117::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8904 CAG18579 trp-3117::Tn10kan OTHER METHODS (Tn10kan conversion) trp-3117::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8905 CAG12111 zci-3118::Tn10kan trp CAG12028(ME8810) OTHER METHODS (Tn10kan conversion) zci-3118::Tn10kan KmR M. Singer requires Trp
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8906 CAG12108 trg-3120::Tn10kan CAG12026(ME8812) OTHER METHODS (Tn10kan conversion) trg-3120::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8907 CAG18576 zdc-3117::Tn10kan CAG18461(ME8813) OTHER METHODS (Tn10kan conversion) zdc-3117::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8908 CAG18567 zdg-3121::Tn10kan CAG18462(ME8815) OTHER METHODS (Tn10kan conversion) zdg-3121::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8910 CAG18568 zdh-3122::Tn10kan CAG12151(ME8816) OTHER METHODS (Tn10kan conversion) zdh-3122::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8911 CAG18518 zdi-3123::Tn10kan CAG18464(ME8817) OTHER METHODS (Tn10kan conversion) zdi-3123::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8912 CAG18578 zdj-3124::Tn10kan CAG18465(ME8818) OTHER METHODS (Tn10kan conversion) zdj-3124::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8913 CAG12122 zea-3125::Tn10kan CAG12074(ME8819) OTHER METHODS (Tn10kan conversion) zea-3125::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803, 4985 ME8915 CAG12126 zeb-3199::Tn10kan OTHER METHODS (Tn10kan conversion) zeb-3199::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8916 CAG18563 zed-3128::Tn10kan CAG18451(ME8823) OTHER METHODS (Tn10kan conversion) zed-3128::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8917 CAG12176 zee-3189::Tn10kan CAG12099(ME8824) OTHER METHODS (Tn10kan conversion) zee-3189::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8918 CAG12100 zeg-3130::Tn10kan CAG12098(ME8826) OTHER METHODS (Tn10kan conversion) zeg-3130::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8919 CAG18577 zeh-3142::Tn10kan CAG12177(ME8827) OTHER METHODS (Tn10kan conversion) zeh-3142::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8920 CAG12183 zei-3143::Tn10kan CAG12178(ME8828) OTHER METHODS (Tn10kan conversion) zei-3143::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8921 CAG18552 zej-3144::Tn10kan CAG18484(ME8829) OTHER METHODS (Tn10kan conversion) zej-3144::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8922 CAG18522 zfb-3135::Tn10kan CAG18467(ME8831) OTHER METHODS (Tn10kan conversion) zfb-3135::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8923 CAG18565 nupC3146::Tn10kan CAG18468(ME8832) OTHER METHODS (Tn10kan conversion) nupC3146::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8924 CAG18632 zfc-3071::Tn10kan OTHER METHODS (Tn10kan conversion) zfc-3071::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8925 CAG18524 purC3137::Tn10kan CAG18470(ME8833) OTHER METHODS (Tn10kan conversion) purC3137::Tn10kan KmR M. Singer requires purines
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8926 CAG18631 zfe-3138::Tn10kan MG1655 OTHER METHODS (Tn10kan conversion) zfe-3138::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8927 CAG18570 zff-3139::Tn10kan CAG18481(ME8835) OTHER METHODS (Tn10kan conversion) zff-3139::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 35627, 29803 ME8929 CAG18608 pheA3141::Tn10kan CAG12158(ME8837) OTHER METHODS (Tn10kan conversion) pheA3141::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8930 CAG18562 zfi-3143::Tn10kan MG1655 OTHER METHODS (Tn10kan conversion) zfi-3143::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8931 CAG12182 cysC3152::Tn10kan CAG12173(ME8839) OTHER METHODS (Tn10kan conversion) cysC3152::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8932 CAG12115 fuc-3154::Tn10kan CAG12079(ME8840) OTHER METHODS (Tn10kan conversion) fuc-3154::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8934 CAG18604 zgd-3156::Tn10kan CAG12168(ME8843) OTHER METHODS (Tn10kan conversion) zgd-3156::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8935 CAG18559 nupG3157::Tn10kan CAG18472(ME8844) OTHER METHODS (Tn10kan conversion) nupG3157::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8937 CAG18574 zgh-3159::Tn10kan CAG12152(ME8847) OTHER METHODS (Tn10kan conversion) zgh-3159::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8938 CAG12127 zgj-3198::Tn10kan CAG12072(ME8848) OTHER METHODS (Tn10kan conversion) zgj-3198::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8939 CAG18605 zha-3168::Tn10kan CAG12153(ME8849) OTHER METHODS (Tn10kan conversion) zha-3168::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8940 CAG12120 zhb-3169::Tn10kan CAG12071(ME8850) OTHER METHODS (Tn10kan conversion) zhb-3169::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8941 CAG18606 zhc-3170::Tn10kan CAG12159(ME8851) OTHER METHODS (Tn10kan conversion) zhc-3170::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8944 CAG18573 zhf-3174::Tn10kan CAG18450(ME8855) OTHER METHODS (Tn10kan conversion) zhf-3174::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8945 CAG18639 zhi-3087::Tn10kan MG1655 OTHER METHODS (Tn10kan conversion) zhi-3087::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8946 CAG12175 zia-3077::Tn10kan MG1655 OTHER METHODS (Tn10kan conversion) zia-3077::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8947 CAG18569 zib-3160::Tn10kan CAG12163(ME8858) OTHER METHODS (Tn10kan conversion) zib-3160::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8948 CAG18572 zic-3161::Tn10kan CAG18492(ME8859) OTHER METHODS (Tn10kan conversion) zic-3161::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8949 CAG18558 zid-3162::Tn10kan CAG18499(ME8860) OTHER METHODS (Tn10kan conversion) zid-3162::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8950 CAG18592 zie-3163::Tn10kan CAG18501 OTHER METHODS (Tn10kan conversion) zie-3163::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8951 CAG18599 ilv-3164::Tn10kan CAG18431(ME8862) OTHER METHODS (Tn10kan conversion) ilv-3164::Tn10kan KmR M. Singer requires ile and val
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8952 CAG18557 fadAB3165::Tn10kan CAG18496(ME8864) OTHER METHODS (Tn10kan conversion) fadAB3165::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8953 CAG18601 zih-3166::Tn10kan CAG18495(ME8865) OTHER METHODS (Tn10kan conversion) zih-3166::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8957 CAG18615 zjb-3179::Tn10kan CAG18498(ME8869) OTHER METHODS (Tn10kan conversion) zjb-3179::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8958 CAG18609 malF3180::Tn10kan CAG12164(ME8870) OTHER METHODS (Tn10kan conversion) malF3180::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8959 CAG12119 malE::Tn10kan OTHER METHODS (Tn10kan conversion) malE::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8960 CAG18630 zjc-3181::Tn10kan MG1655 OTHER METHODS (Tn10kan conversion) zjc-3181::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8961 CAG18571 zjd-3182::Tn10kan CAG18488(ME8871) OTHER METHODS (Tn10kan conversion) zjd-3182::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8962 CAG18555 zje-3183::Tn10kan CAG18427(ME8872) OTHER METHODS (Tn10kan conversion) zje-3183::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8963 CAG12114 cycA3185::Tn10kan CAG12073(ME8873) OTHER METHODS (Tn10kan conversion) cycA3185::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8964 CAG12110 zjh-3186::Tn10kan CAG12019(ME8874) OTHER METHODS (Tn10kan conversion) zjh-3186::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8965 CAG18610 zji-3187::Tn10kan CAG18429(ME8875) OTHER METHODS (Tn10kan conversion) zji-3187::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8966 CAG18619 zjj-3188::Tn10kan CAG18430(ME8876) OTHER METHODS (Tn10kan conversion) zjj-3188::Tn10kan KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8968 CAG18637 zdf-3062::Tn5 MG1655 zdf-3062::Tn5 KmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8969 CAG12027 zdd-230::Tn9 PK1220 MG1655 zdd-230::Tn9 CmR M. Singer Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME8970 CAG12033 M. Singer wild type from MS8002 MG1655(B.Bachmann)
Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain. 29803 ME5491 HfrT43 Hfr F HfrP4X(proB -> lac) thi dnaB43 P4X8 CR34BT43 CROSS Hfr Lac+ Met+ 36018 ME5494 Hfr432-19 Hfr F HfrP13(pyrE <- dnaA) malA thr leu arg trp str lac mtl dnaB432 P13 JE10432 CROSS Hfr Xyl+ Cys+ ME6038 LE392 F- hsdR514(RK- MK-) supF58 lacY1 or DE(lacIZY)6 galK2 galT22 metB1 trpR55 (lambda)- supE44 ED8654 ME6039 MAL103 F- Mu.ctsDE((proAB-lacIPOZYA)XIII) strA Mu.dl(ApR,lac) 29726 ME6040 QDsup3 F- supIII pro host strain of T4GT7 30084, 30066 ME6041 LC248(pTT245) Hfr F HfrKL16-99(thy -> serA,F) Other recA1 thi (lambda)- B. Low pTT245:(dnaX+, dnaZ+, TcR from pBR322) ME6042 PNS1 Other versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the CI gene of coliphage 434 30023 ME6043 LC169 F+ F DE(proB-lac) ME6044 LC102 R100+ F- Other ile argG ara purE xyl mtl leu proC gal str tsx lac trp(E?) thi his metB ME6045 LC102 R100+-1 F- Other gal proC leu purE trp(E?) thi his metB ile argG ara lac xyl mtl str tsx ME6046 JF390 Other unknown marker host Foulds ME6047 W3350 F- galK galT (lambda)- Toshio Nagata ME6048 W3350(P2) F- Other p2 galK galT (lambda)- Toshio Nagata ME6050 ED2446 F- F- trp spc his DE((proB-lac)XIII) ED2446 CURING/Temp. lac- F- Lac- at 42C ME6051 2300 U 178M F- arg his thi str xyl lambda_R lacI3 lacZ178 Y. Ohshima ME6052 2300 LacZ lambda_S F- Su- arg? lacZ lambda_S P13 ME6051(=2300 U 178M) CROSS xyl+ lambda_S Su- Xyl+ Cys+ His+ most probably lacI3 ME6054 CSH44C F- λ+ cured DE(lac) tonA thi ME6053 CURING/Temp. lambda_- Lac- at 40C ME6055 ED2446 F-/F lac F+ F trp spc DE(lac-pro)XIII his LC169 ME6050(ED2446 F-) CROSS F-lac Lac+ SpcR ME6056 ED2446 F-/Ft62 lac F+ F trp spc DE(lac-pro)XIII his 200PSFt62Lac ME6050(ED2446 F-) CROSS Ft62-lac Lac+ SpcR at30C ME6057 LC343/F lac F+ F leu DE(proB-lac) dnaA(T46) thy ME6055 (ED2446 F-/F lac) LC343 CROSS F-lac Lac+ His+ Trp+ ME6058 LC343/Ft62 lac F+ F leu DE(pro-lac) dnaA(T46) thy ME6056 (ED2446 F-/Ft62 lac) LC343 CROSS lac+ Lac+ His+ Trp+ ME6060 XA35 Mal+ F- lac str mal+ Mal+ lacI3,O+,P+,ZdelM111,Y+(90%expression) ME6061 CRT4624/F lac F+ F arg dnaA(T46) thr leu thi lacY xyl malA mtl ara galB str tonA ME6055 (ED2446 F-/F lac) CRT4624 CROSS F-lac+ Lac+ StrR ME6062 CRT4624/Ft62 lac F+ F leu arg lacY dnaA(T46) thr xyl malA mtl ara galB str tonA thi his ME6056 (ED2446 F-/Ft62 lac) CRT4624 CROSS Ft62-lac Lac+ StrR Transfer frequency of Ft62-lac is very low. ME6063 W4573 RecA F- gal xyl str recA ara lac LC248 JE1024 CROSS thy+ recA- Thy+ StrR ME6064 CRT46/Ft62 lac F+ F leu thi thy ilv lac mal (lambda)- dnaA(T46) thr ME6056 (ED2446 F-/Ft62 lac) CRT46 CROSS Ft62-lac+ Lac+ His+ Trp+ Thr marker is leaky ME6065 CRT46/F-lac F+ F leu thi thy ilv lac malA dnaA(T46) thr ME6055 (ED2446 F-/F lac) CRT46 CROSS F-lac+ Lac+ His+ Trp+ ME6066 ED2446 F-/Ft113-lac F+ F tsx DE(lac-pro)XIII his trp spc 200psFt113lac ME6050 (ED2446 F-) CROSS Lac+ SpcR ME6068 HFR P13 LacZ Hfr F HfrP13(pyrE <- dnaA) lambda_S arg? thi? str lacZ P13 ME6051(=2300 U 178M) CROSS Xyl+ Cys+ His+ ME6069 W4573/Ft62 lac F+ F gal xyl str ara lac ME6058 (LC343/Ft62 lac) W4573 CROSS Lac+ StrR at 30C ME6070 TK405M F Lac F+ F gal malA kdpABC5 trkA trkD rha lacZ ME6055 (ED2446 F-/F lac) TK405M CROSS F-lac+ Lac+ His+ Trp+ ME6071 LC343 R F- DE(proB-lac) thy leu LC343 SPONTANEOUS TR TR ME6072 LC343 S-3 Hfr F Hfr(polC -> proA) leu DE(proB-lac) dnaA(T46) thy ME6057 (LC343/F lac) integrated F-lac TR phenotype lac+ TR ME6073 LC343 S-6 Hfr F Hfr(xyl -> ile) leu DE(proB-lac) dnaA(T46) thy ME6057 (LC343/F lac) integrated F-lac TR phenotype lac+ TR ME6074 LC343 S-8 Hfr F Hfr(gal,his -> xyl) leu DE(proB-lac) dnaA(T46) thy ME6057 (LC343/F lac) integrated F-lac TR originally high fertility, later no fertility phenotype Lac+ TR ME6075 CRT46R F- thi thr leu thy ilv lac mal CRT46 SPONTANEOUS TR TR true revertant of suppressor mutation ME6078 W4573LysA F- mtl gal str lysA lac ara xyl LC607 JE1024 lysA Thy+ ME6079 F- AroE F- DE(pro-lac)XIII his str aroE trp MA1065argG+aroE- ME6050 (ED2446 F-) CROSS StrR PurC+ ME6080 AroE/F-lac F+ F DE(pro-lac)XIII trp aroE his str ME6055 (ED2446 F-) ME6079 (F- AroE) CROSS Lac+ StrR ME6081 F- DapC F- dapC str AT997 W4100 CROSS StrR ProA+ ME6082 E5014Rif F+ F supE50 thi rel mal spc-12 DE(proB-lac)XIII E5014 SPONTANEOUS Rif (50_micro_g/ml) Co-td; argH/rif : 42% mutation at rpoB gene ME6083 AroE Spc/F-lac F+ F trp spc aroE his DE(pro-lac)XIII E5014 ME6080 (AroE/F-lac) SpcR ME6085 CD-4 Hfr F HfrC proA3 malA38 lac-3 metD88 tsx-76 metB1 W. Epstein no growth on D-methionine 30182, 29752 ME6086 PC34/F-lac F+ F dnaG thi leu purE his str lac can ME6055 (ED2446 F-/F lac) PC34 CROSS StrR Lac+ ME6087 F- DE(lac-pro) thi LC169 SPONTANEOUS Lac- ME6088 CR34T2 F- thy thr lac leu thi CR34 SPONTANEOUS growth on 2_micro_g/ml thymine ME6089 FRAG-5 F- gal lac kdpA,B,C5 thi rha W. Epstein ME6090 TK110 F- gal lac kdpA,B,C5 trkB110 thi rha W. Epstein ME6091 LU-53 F- glpR lacI3 strA galR W. Epstein ME6092 LU-53 Crp 5a F- glpR strA galR lacI3 crp5a W. Epstein 55810 ME6093 CFE-11 Hfr F Hfr312 lac thi str crp5a leu thr W. Epstein ME6094 LC148/F-lac F+ F DE(pro-lac) leu thy ME6055 (ED2446 F-/F lac) LC148 CROSS Lac+ Trp+ His+ thy: 2_micro_g/ml ME6095 LC169 AroE F+ F str DE(proB-lac) aroE MA1065AroEArgG+ LC169 StrR ME6096 LC169AroESpc F+ F spc DE(proB-lac) aroE E5014 ME6095 (LC109 AroE) SpcR ME6097 LC169 AroE+ Spc F+ F DE(proB-lac) spc E5014 ME6095 (LC169 AroE) SpcR ME6099 LC148/F15 F+ F DE(pro-lac) thy leu LC102thy-/F15 LC148 Thy+ PurE+ Trp+ His+ Arg+ Ile+ MetA+ ME6102 TK110/Flac F+ F rha kdpA,B,C5 gal lac trkB110 thi LC169 ME6090 (TK110) CROSS F-lac+ Lac+ Pro+ ME6103 FRAG-5/Flac F+ F lac kdpA,B,C5 gal rha thi LC169 ME6089 (FRAG-5) CROSS F-lac+ Lac+ Pro+ ME6104 W3110(lambda_857) F- λ+ lambda_cI857 IN(rrnD-rrnE)1 lambda_cI857 W3110 lambda_-immune grow at 30C lyse and releare _lambda_ phage at 42C ME6105 TK405M T46 F- gal? mal? dnaA(T46) kdpA,B,C5 trkA trkD lac? HfrT46P13(Y. Takeda) TK405M CROSS dnaA(T46) Rha+ Thr+ Leu+ His+ ME6106 TK405M TrkD+ F- trkA kdpA,B,C5 lac? mal? gal? HfrT46P13 TK405M CROSS trkD+ Rha+ Thr+ Leu+ His+ ME6107 TK405M Rha+ F- gal? kdpA,B,C5 trkA trkD lac? mal? HfrT46P13 TK405M CROSS Rha+ Thr+ Leu+ His+ ME6108 TK405M T46 TrkD+ F- dnaA(T46) lacZ gal? mal? trkA kdpA,B,C5 HfrT46P13 TK405M CROSS Rha+ Thr+ Leu+ His+ ME6109 TK405M T46D+/Flac F+ F trkA dnaA(T46) gal? mal? lacZ kdpA,B,C5 LC169 ME6108 (TK405M T46 TrkD+) CROSS Lac+ Pro+ ME6110 ER Thy F- thyA asn thi ER OTHER METHODS (Trimethoprim) thyA Thy- ME6111 ER Thy SM F- thi asn thyA str ME6110 (ER Thy) str StrR ME6112 ER RecA SM F- asnB32 recA1 str thi asnA31 LC248 ME6111 (ER Thy SM) CROSS recA Thy+ StrR 30026 ME6114 E. coli K-235 F- Other prototroph Y. Takagaki ME6115 W2252SMRMet-(ColE2) F- Other Y. Takagaki ME6116 20 SO F- malA or B thi str lacZ Y. Ohshima ME6117 CA38(ColE3 ColI) Other Y. Takagaki ME6118 OB10Rec-3623 Other gal trp rec Y. Takagaki ME6119 45A69/T6R F+ F lac DE(trp)A T6R DE(trp)B T1R thi Y. Takagaki SmS ME6120 W3110 SMR (ColE3-CA38) Other IN(rrnD-rrnE)1 Y. Takagaki ME6121 K12-317 Other Reeves ME6122 M-K260 Other Reeves 29781 ME6123 B-K260 Other Reeves 29781 ME6124 D-CA23 Other Reeves 29781 ME6125 Ib-P9 Other Reeves 29781 ME6126 E3-CA38 Other Reeves 29781 ME6127 X-K235 Other Reeves 29781 ME6128 J53/R386 F- Other met pro N. Datta ME6129 J53/R1 F- Other pro met N. Datta ME6130 J53/R124 F- Other pro met N. Datta ME6131 J53-1/Sa F- Other met pro N. Datta ME6132 J53/R16 F- Other pro met N. Datta ME6133 J53/R6K F- Other pro met N. Datta ME6134 J53/R483 F- Other pro met N. Datta ME6135 J53/R391 F- Other met pro N. Datta ME6136 J53/RP4 F- Other pro met N. Datta ME6137 J53NalR/RA1 F- Other met pro nal C. Monti-Bragadin 29787 ME6138 J53/JR66A F- Other pro met C. Monti-Bragadin 29787 ME6139 J53/R1 F- Other met pro C. Monti-Bragadin 29787 ME6140 J53/R391 F- Other pro met C. Monti-Bragadin 29787 ME6141 J53NalR/pTM89 F- Other nal pro met C. Monti-Bragadin 29787 ME6142 J53/R16 F- Other met pro C. Monti-Bragadin 29787 ME6143 J53/R6K F- Other met pro C. Monti-Bragadin 29787 ME6144 J62/Hly F- Other his pro trp C. Monti-Bragadin 29787 ME6145 J53/R387 F- Other met pro C. Monti-Bragadin 29787 ME6146 J53NalR/R64 F- Other nal met pro C. Monti-Bragadin 29787 ME6147 J53/RP4 F- Other met pro C. Monti-Bragadin 29787 ME6148 J53NalR/Sa F- Other nal met pro C. Monti-Bragadin 29787 ME6149 J53NalR/R124 F- Other pro met nal C. Monti-Bragadin 29787 ME6150 C600/Fo-lac Other lacY thr leu thi C. Monti-Bragadin 29787 ME6151 J53NalR/pTM338 F- Other pro met nal C. Monti-Bragadin 29787 ME6152 J53/R401 F- Other pro met C. Monti-Bragadin 29787 ME6153 J53NalR/R69(IP) Other pro met nal C. Monti-Bragadin 29787 ME6154 J53/R57-6 F- Other met pro C. Monti-Bragadin 29787 ME6155 J53/R483 F- Other met pro C. Monti-Bragadin 29787 ME6156 J53/R46 F- Other pro met C. Monti-Bragadin 29787 ME6157 J53NalR/ColB-K98 F- Other nal met pro C. Monti-Btagadin 29787 ME6158 TK405M Spc F-lac F+ F trkA405 trkD rha gal lacZ malA kdpABC5 JE6082 JE6070 spc SpcR no growth on K=0.1mM ME6159 TK405M Spc Trk+/F-lac F+ F gal lacZ malA kdpABC5 trkD rha ME6082 (E5014Rif) ME6070 (TK405M F Lac) spc trkA+ SpcR growth on K=0.1mM ME6160 E110 F- argE3 str-31 tsx-33 sup-37 (lambda)- thr-1 proA2 mtl-1 xyl-5 ara-14 galK2 glmS lacY1 thi-1 leu-6 his-4 requires 200_micro_g/ml glucosamine E110 = AB1157 glmS 30014 ME6161 TK110 Spc TrkB+/F-lac F+ F kdpABC5 thi rha gal lac spc ME6082 (E5014Rif=JE5592) ME6102 (TK110/Flac) trkB+spc SpcR ME6162 TK110 Spc/F-lac F+ F spc thi rha gal lac kdpABC5 trkB ME6082 (E5014Rif=JE5592) ME6102 (TK110/Flac) spc SpcR ME6163 C600 SmR (pSC138) Other lac thi thr leu pSC138 DNA C600 Str TF. ApR Ap 20_micro_g/ml 29780 ME6165 N611 F- polA(N611) trp str gal W3623 MUTAGENESIS/NTG M. Sekiguchi casamino acid requiring 29947 ME6166 N611 T20 F- thy trp gal str polA(N611) ME6165 (N611) SPONTANEOUS thy- trimethoprimR requires casamino acid and thymine (20_micro_g/ml) ME6167 N611 T2 F- gal trp thy polA(N611) str ME6166 (N611 T20) SPONTANEOUS thy- (2_micro_g/ml) growth on 2_micro_g/ml thymine requires casamino acid and thymine (2_micro_g/ml) ME6168 N611 HfrC Hfr F HfrC thy polA(N611) W2252 ME6166 (N611 T20) CROSS HfrC Gal+ Met+ SmR Trp+ ME6169 C600 SM T20 F- thy str lacY thi leu thr C600 Str thy- trimethoprim resistant requires 20_micro_g/ml thymine ME6170 C600 SM T2 F- str thr leu thi lacY thy ME6169 (C600 SM T20) SPONTANEOUS thy- (2_micro_g/ml) growth on thymine 2_micro_g/ml requires 2_micro_g/ml thymine ME6171 C600 SM T2 (pSC138) F+ Other str thy thr leu thi lacY pSC138 DNA ME6170 (C600 SM T2) TF. pSC138 ApR 20_micro_g/ml 29780 ME6172 LC343(pSC138) F+ Other dnaA(T46) thy leu DE(proB-lac) pSC138 DNA LC343 TF. pSC138 ApR 20_micro_g/ml 29780 ME6173 CRT46 SM PolA(N611) F- leu thi thy str dnaA(T46) polA(N611) thr ME6168 (N611 HfrC) CRT46 Spc CROSS polA(N611) Ilv+ StrR ME6177 N4752 F- leu sts-4752 str gal rns-19 cys D. Apirion 29993 ME6178 N5352 F- sts-4752 str spc gal rns-19 leu cys D. Apirion 29993 ME6179 #153(lambda_Tna+) λ+ lambda_tnaI21 supF tnaA DE(trpA-E) tonB W. Brammar -> Y. Hirota UV-inducible, plaque former 29772 ME6180 LS670(phi_80 Tna+Bgl+) φ80- (phi_80hdtna+ bgl+) (phi_80h) metE gal ilvA rbsK L. Soll -> Y. Hirota defective UV-inducible ME6181 803 SuIII+ RecA F- recA met supF hsdR hsdM M. Sugiura ME6183 CV530C F- leuA371 DE(lac-pro) thi CV530 CURING/Temp. lambda_- 42C survivor CV530 = CV437(lambda_pleu12 S7 cI857) 29734 ME6185 W3110(lambda_imm21 nin5) F- λ+ lambda_imm21 nin5 IN(rrnD-rrnE)1 lambda_imm21 nin5 W3110 lambda ME6186 JC7623 F- xyl-5 leu-6 thr-1 proA2 mtl-1 his-4 ara-14 galK2 lacY1 thi-1 recB21 recC22 sbcB15 argE3 str-31 tsx-33 supE344 A. J. Clark ME6187 JC8111 F- str-31 tsx-33 supE344 recB21 recC22 recF143 sbcB15 lacY1 thi-1 leu-6 thr-1 xyl-5 ara-14 galK2 mtl-1 proA2 his-4 argE3 A. J. Clark ME6188 JC8411 F- str-31 thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 tsx-33 supE344 recF143 A. J. Clark ME6189 JC8171 F- str-31 tsx-33 supE344 recB21 recC22 recL152 sbcB15 argE3 his-4 proA2 mtl-1 xyl-5 ara-14 galK2 lacY1 thi-1 leu-6 thr-1 A. J. Clark ME6190 JC8471 F- str-31 tsx-33 supE344 recL152 thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 A. J. Clark ME6191 JC5495 F- thi-1 thr-1 leu-6 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 str-31 tsx-33 supE344 recA13 recB21 A. J. Clark ME6192 JC5544 F- recC22 tsx-33 str-31 supE344 recA13 thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 A. J. Clark ME6193 JC5547 F- recC22 his-4 thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 argE3 str-31 tsx-33 supE344 recA13 recB21 A. J. Clark ME6194 SP9Mal+ F- his met bglA7 bglB13 bglR2 arg ilv thi W3110 SP9 mal+ Mal+ ME6195 SP10Mal+ F- his bglA7 bglB13 bglR1 bglS4 thi met arg W3110 SP10 mal+ Mal+ ME6196 KL141mal+ F- argG gadR str pyrE relA1 thi-1 gadS gltS rbs thyA W3110 KL141 mal+ Mal+ KL141 is malA ME6197 CV530C Str F- leuA371 thi str DE(lac-pro) LC607 ME6183 (CV530C) str StrR ME6198 F- DE(pro-lac) leuA thi DE(gal bio) DB530 (lambda_cI857R) CURING/Temp. lambda_-gal-bio- 42C possibly deleted at gal-bio region ME6199 CV530C Gal- F- leuA371 bio gal thi DE(lac-pro) ME6183(lambda_cI857 Xisl) lambda_-gal-bio- 42C possibly deleted at the gal-bio region ME6200 CV530C Gal- UvrB- F- thi DE(gal-bio-uvrB) DE(lac-pro) leuA371 ME6183 (CV530C) gal- bio uvrB 42C S. Yasuda Grow poorly on L agar at high temp ME6203 CV530C Hfr11 Hfr F HfrP4X(argF -> lac) rifR leuA371 GF11 (Y. Nishimura) ME6183 (CV530C) CROSS RifR Trp+ Pro+ Lac+ ME6204 CV530C Hfr 93 Hfr F HfrP4X(argF -> lac) leuA371 rif GF93 ME6183 (CV530C) CROSS RifR Trp+ Pro+ Lac+ ME6205 MO611 T46 str thi pro trp his thr dnaA(T46) leu HfrT46P13 MO611 CROSS Arg+ StrR (lac arg)? ME6206 LC256 Rif F- leu thy ilv rif ME6082 (E5014 Rif) LC256 rif RifR (50_micro_g/ml) ME6207 CRT46 Rif F- mal rif leu thy ilv dnaA(T46) thi lac thr ME6082 (E5014 Rif) CRT46 RifR (50_micro_g/ml) ME6208 W3110(lambda_cI857Sam7) F- λ+ lambda_cI857 Sam7 IN(rrnD-rrnE)1 lambda_cI857Sam7 phage W3110 lambda_R ME6209 C600SMT2(lambda_cI857Sam7) F- λ+ lambda_cI857Sam7 thr thi str thy leu lambda_cI857Sam7 ME6170 (C600 Sm T2) lambda_R ME6210 F- recA leu ilv rif LC248 ME6206 (LC256 Rif) CROSS RifR Thy+ ME6212 CRT46 SM Ilv+ TR F- thi thy str spc mal lac thr leu ME6111 (ER Thy Sm) CRT46 Spc Ilv+ ME6213 CRT46SmTR F- thy ilv str spc mal lac thi leu thr ME6111 (ER Thy Sm) CRT46 Spc TR ME6215 HfrP4X T46-19 Hfr F HfrP4X8 dnaT46 ilv str thi leu thr HfrP4X8 CRT46 Spc StrR Lac+ ME6217 HfrP4X T46-15 Hfr F HfrP4X8(argF -> lac) str HfrP4X8 CRT46 Spc Lac+ StrR ME6218 CRT46 Rif RecA-2 F- recA thr leu ilv dnaA(T46) LC248 ME6207 (CRT46 Rif) CROSS dnaA recA Thy+ RifR (lac mal)? ME6221 HfrP4X Ilv TL Met Str Hfr F HfrP4X8(argF -> lac) thr str ilv met leu HfrP4X8 ME6213 (CRT46 Sm TR) CROSS Lac+ StrR ME6222 HfrP4X T46 SM RecA-2 Hfr F HfrP4X(argF -> lac) recA str ilv LC248 ME6214 (HfrP4X/T46-1) CROSS Thy+ StrR ME6223 W3110(P2) Other P2 IN(rrnD-rrnE)1 H. Yamagishi ME6224 W3110(lambda_gt.lambda_c) F- λ+ lambda_gt.lambda_c IN(rrnD-rrnE)1 lambda_gt.lambda_c W3110 lambda_gt.lambda_c phage; H. Yamagishi heat inducible 29876 ME6225 AN120 F- argE3 str uncA401 thi-1 AN119 JP58(J. Pittard) Ilv+ J. D. Butlin, Anraku, Takagaki 29877 ME6226 LC248 T2 Hfr F HfrKL16(thyA -> serA) thi recA thy LC248 T20 growth on 2_micro_g/ml thymine growth on 2_micro_g/ml thymine ME6228 BK269 F+ Other thi str DE(leu-pro) ara B. C. Kline 29740 ME6229 BK272 Other ara DE(leu-pro) str thi B. C. Kline 29740 ME6232 CR34(pML31) Other tonA supE44 thy lac thi leu thr D. Helinski strS 29771 ME6242 JE5525 R100-1 Other pps recA1 man lpm trp gal str A. Nishimura ME6244 JE5525 R+538 F drd Other str recA1 trp gal man pps lpm ME6250 Hfr F HfrP4X8(argF -> lac) dnaB(T42) grpP ME6252 LC248 Hfr F HfrKL16(thyA -> serA) (lambda)- recA1 thi ME6253 P3478 F- deoC2 (lambda)- IN(rrnD-rrnE)1 polA1 thyA36 30041, 29966 ME6256 PA3364 his thi xyl mtl str lambda_R endI26 polA sup+ ME6257 LA5325 F- recA thi DE(proB-lac) leuA rif ME6259 JE6112 Thy F- thy str thi asn recA A. Nishimura ME6266 LC607 PolA11 F- lys polA11 purE trp proC leu thi lacZ xyl ara tonA tsx str W3623PolA11 LC607 PolA11 Met+ A. Nishimura ME6267 LC607 PolA12 F- thi leu xyl proC lys trp purE lacZ ara tonA str tsx polA12 N1126 LC607 PolA12 Met+ A. Nishimura ME6268 LC607 PolA1 F- tsx polA1 ara xyl lacZ tonA thi leu proC lys trp purE str PA3364PolA1 LC607 PolA1 Met+ A. Nishimura ME6270 LC607 PolA107 F- tsx trp lys proC leu thi lacZ xyl ara tonA str purE polA107 KL406 LC607 PolA107 Met+ A. Nishimura ME6271 KL406 thi leu polA107 malA hisF thyA cysC lacZ ara mtl xyl str spc proC 855 ME6272 ER(F-) F- thi asn ER CURING/AO F- (Q_beta_R) A. Nishimura ME6274 F- str asn thy ME6111 (ER Thy Sm) CURING/AO F- (Q_beta_R) A. Nishimura ME6276 F- thi asn recA str LC248 ME6274 recA- Thy+ SmR A. Nishimura ME6279 F- asn thi thy str recA ME6276 MUTAGENESIS/Others (trimethoprime) thy- trimethoprimeR A. Nishimura thy(2_micro_g/ml) ME6299 PYT10 Hfr F HfrP4X8(argF -> lac) ME7101 N1126 str-109 xyl-5 mtl-1 ara-14 lacZ36 cysC43 thyA54 hisF860 malA38 proC32 leu-6 thi spc-15 polA12 29927 ME7260 JC411 F- supE44 tonA2 thy deo polAts214 leuB6 hisG1 argG6 metB1 lacY1 gal-6 xyl-7 mtl-2 malA1 rpsL104 lambda_R (lambda)- thy (20_micro_g/ml) 29912, 29906, 863, 29942, 29965 ME7741 GM31,CGSC5125 tsx supE tonA str mtl xyl galKT lac ara thi his dcm leu thr A. Oka CGSC(B.Bachmann & M.Berlin) 29919 ME7744 GM31 Thy- ara thr leu dcm his thi lac galKT xyl mtl str tonA tsx supE thy thy- TrimethoprimeR A. Oka 29919 ME7745 GM31 RecA F- galKT xyl supE tonA tsx str mtl recA thr leu dcm his thi ara lac LC248 ME7744 (GM31 Thy-) CROSS recA Thy+ SmR A. Oka 29919 ME7760 Lin6 Hfr F HfrC(purE -> lip) thi glpT ME7761 W620 F- rel-1 supE44 galK30 thi-1 pyrD36 str-129 ME7762 LC161 F- str leu argH tsx (lambda)- proA ME7763 CV-2 F HfrC(purE -> lip) plsA2(=adk-2) glpD3 glpRC2 phoA8 tonA22 T2R rel-1 (lambda) Ts mutant 29895 ME7764 W3899 F- nad Cavalli 257 nad(=nic) ME7765 BW9093 F- leu-6 thr-1 str-31 argE3 his-4 galK2 xthA3 30083, 30073, 29911 ME7766 W3110 CysB cysB ME7767 W4573 F- T6S lac-85 gal-2 str mtl xyl-2 mal-1 ara-2 ME7768 W3637 Spc1 F- metB1 spc-1 (lambda)- ME7769 LC102 F- mtl gal proC tsx str leu purE trp(E?) thi his metB ile argG ara lac xyl ME7770 LC193#1 F- trp purE metA or metB proC leu thi his ile argG lacY xyl ara mtl gal malA tsx str (lambda)- lambda_R ME7771 LC256 F- ilv thy leu ME7772 LC607 F- str (lambda)- lambda_S ara tsx tonA xyl lacZ thi leu proC metE lys trp purE 29981 ME7774 AB3303 Gal+ F- argE3 his-4 str-700 or str-704 tsx-29 or tsx-358 lacY1? galK2? xyl-5? mtl-1? pabB3 thi-1 ME7777 W4637 F- T6R (lambda)- pur lac-85 ME7778 W3110 F- IN(rrnD-rrnE)1 B. Bachman -> Kohara -> Nishimura Sigmatype A, slow growth on high level adenine ME7779 W4100 F- λ+ proA or B (lambda)+ str ME7780 AT1325 Lip-9 F- proA2 his-4 thi-1 str-35 purB15 mtl-1 galK2 xyl-5 lacY1 supE44 lip-9 29955 ME7781 CRT266 F- str-109 thi-1 metE70 leu-6 proC32 malA38 hisF860 thyA54 cysC43 lacZ36 ara-14 mtl-1 xyl-5 spc-15 single colony#1 ME7783 W2252 Hfr F HfrC(purE -> lip) (lambda)- metB 30002 ME7784 P4X8 Hfr F HfrP4X8 (lambda)- metB ME7785 610 Hfr F HfrIOR-3 leu (lambda)- thy ME7786 JC1553/KLF10,CGSC4261 F+ F his-1 metB1 argG6 xyl-7 tsx-1 lambda_R (lambda)- supE44 str-104 recA1 lacY1 gal-6 malA1 tonA2 mtl-2 leu-6 CGSC(B.Bachmann & M.Berlin) This strain must be grown on medium containing arg his leu and lacking met ME7787 JC1553 F- tonA2 argG6 metB1 his-1 leu-6 recA mtl-2 xyl-7 malA1 gal-6 lacY1 str-104 tsx-1 lambda_R (lambda)- supE44 JC1553/KLF10 CURING/AO CGSC(B.Bachmann & M.Berlin) ME8001 F143,CGSC4291 F+ F lambda_R thi-1 tyr-2 pyrD34 his-68 trp-45 thyA33 recA1 mtl-2 xyl-7 malA1 galK35 strA118 (lambda)- KL259 K. B. Low, CGSC(B.Bachmann & M.Berlin) ME8002 KL16,CGSC4245 Hfr F HfrKL16-PO45(thyA -> serA) relA1 thi-1 (lambda)- K. B. Low, CGSC(B.Bachmann & M.Berlin) 29942 ME8003 F142,CGSC4279 F+ F galK35 thi-1 tyrA2 pyrD34 his-68 trp-45 recA1 mtl-2 xyl-7 malA1 strA118 lambda_R (lambda)- KL253 K. B. Low, CGSC(B.Bachmann & M.Berlin) ME8004 MX419,CGSC5350 Hfr F HfrPO44-S(his -> mgl) thi-1 relA1 lacZ2210 nalA21 supD74 (lambda)- tsx-85 strA183 M. Oeschger, CGSC(B.Bachmann & M.Berlin) 29770 ME8005 SB1803,CGSC4929 F- supE44 (lambda)- metG83 leu-6 thr-1 thi-1 hisC3 proA2 mtl-1 xyl-5 ara-14 galK2 lacY1 strA25 B. Blumenthal, CGSC(B.Bachmann & M.Berlin) 29887 ME8006 KL983,CGSC4240 Hfr F HfrKL983-PO53(dsdA -> supN) (lambda)- mglP1 lacY1 xyl-7 K. B. Low, CGSC(B.Bachmann & M.Berlin) ME8007 F198,CGSC4344 F+ F (lambda)- proC43 ptsI40 bglB6 recA1 strA150 relA1? FF7040 W. Epstein, CGSC(B.Bachmann & M.Berlin) ME8008 594Srl(LSRL110(lambda_d Srl)recA44#13) F- λ+ sup+ (Su-) srl 594 Srl H. Ogawa ME8009 CA274 Hfr trp(am) lac(Am) J. Verten ME8010 H680,CGSC5038 F- tyrA2 his-68 trp-45 purB51 lacY1 gal-6 str-125 tonA2 tsx-70 supE63 lambda_R (lambda)- xyl-7 mtl-2 malA1 thi-1 P. G. Dehaan, CGSC(B.Bachmann & M.Berlin) H680 = PC0254 ME8011 YAA1,CGSC5011 F- lambda_R thi-1 his-68 trp-45 mtl-2 xyl-7 malA1 galK35 fabA2 str-118 (lambda)- J. Cronan JR., CGSC(B.Bachmann & M.Berlin) 29898 ME8012 LA12,CGSC5084 F+ F ptsG1 thi-1 rel-1 (lambda)- B. Magasanik, CGSC(B.Bachmann & M.Berlin) 30000 ME8013 W4597 F8 F+ F galU W4597 T. Fukasawa ME8015 W3350 F- gal-1 gal-2 T. Icho ME8016 M65(lambda_cI857.S7) F- λ+ lambda_cI857.S7 gal ton strA M65 T. Icho 29956 ME8017 AT2531,CGSC4516 F- argH1 supE44? tsx-23 or -25 his-1 purF1 mtl-2 xyl-7 malA1 ara-13 lacY1 or Z4 str-8,-9 or -14 tonA2 or -14 serA24 thi-1 A. L. Taylor ,CGSC(B.Bachmann & M.Berlin) D-serine sensitive ME8018 PA335 F- his-1 str-9 lacY1 thi-1 supE44 argH1 leu-6 thr-1 gal-6(galb) tonA2 mtl-2 ara-13 xyl-7 malA1 T. Icho ME8019 X'181A,CSH59 F- pyrC trp strA thi CSH(J.H.Miller) ME8020 C600,CR34 F- lacY1 thr-1 leu-6 thi-1 supE44 tonA21 ME8021 KL96 Hfr F HfrKL96-PO44(his -> mgl) spoT1 thi-1 rel-1 ME8022 JA200 F+ F DE(trpE)5 recA lacY thi gal xyl ara mtl thr-1 leu-6 J. Carbon 29773 ME8023 159_lambda F- λ+ lambda_ind- uvrA157 galK2 rpsL200 H. Muriaido M. Silverman 29873 ME8024 X178,CSH48 F+ F φ80- his thi CSH(J.H.Miller) ME8025 AB4090,CGSC4090 F- tsx-61 (lambda)- ilv-276 tdk-1 CGSC(B.Bachmann & M.Berlin) 29975 ME8026 JK266,CGSC5473 F+ F trpA62 dadR1 (lambda)- purB56 trpE61 tna-5 J. Kuhn, CGSC(B.Bachmann & M.Berlin) ME8027 CSH54,X715 F- thi strA his DE(lac-pro) pyrF trp supF(suIII) ME8028 CR63 His-1 SupD60 F+ F his supD60 CR63 T. Icho ME8029 CA167,CGSC5401 Hfr F HfrPO1(vals <- attp4) lacZ13 lacI22 relA1 thi-1 supC70 S. Brenner, CGSC(B.Bachmann & M.Berlin) ME8030 CSH25A F- φ80- phi_80H supF SupF = (SuIII) ME8031 ND40,CGSC5215 F+ F trp-49 DE(lac4680) str recA E. Ohtsubo, CGSC(B.Bachmann & M.Berlin) ME8032 YA149,CGSC4500 Hfr F HfrPO1(valS <- attP4) (lambda)- rel-1 thi-1 pyrF40 Hfr3000 F. Jacob, CGSC(B.Bachmann & M.Berlin) YA149 = AT2242 30175 ME8033 W3110(lambda_cI857) F- λ+ lambda_cI857 IN(rrnD-rrnE)1 T. Icho ME8034 LC611(ColV2+) F- Other azi thr leu thi colE1R colVR LC611 Y. Takeda ME8035 Q13 Hfr F pnp tyr (lambda)+ met rna D10 K. Isono 29937 ME8036 KH802R-MHSR F- supE lacY met galK hsdR KH802 W. Blattner hsdR- hsdM+ ME8037 DF1647 F- his tyr rpsL edd pyrD DF10 D. Fraenkel 29938 ME8038 KM1593 F- uvrA157 galK2 supF rpsL200 159 K. Macentee W. Epstein ME8039 WD5021A F- rpsL gal-2 gal-1 ME8039 = 594 M. Silverman ME8040 KM1591 F- uvrA157 rpsL200 galK2 supD 159 K. Macentee W. Epstein ME8041 X7026 F- DE(lac-pro) thi supE M. Silverman ME8042 SF8 F- recB hsdR hsdM recC lop-11 supE44 gal-96 rpsL leuB6 thi-1 thr J. Zieg ME8043 RP445 F- thi thr(Am) leu his met(Am) ara xyl mtl eda rpsL J. Rarkinson ME8044 AW569 F- tar thi lac gal-1 gal-2 xyl ara rpsL tonA tsx tsr his leu K. Oosawa - Y. Imae ME8045 EJ798 F- tyr(am) lac(Am) tonA(Am)? tsx(Am) bfe thyA gyrA his ilv rpsL trp(am) EJ2001 Y. Komeda ME8046 KL188,CGSC4211 F- pyrD34 his-68 trp-45 thyA25 mtl-2 xyl-7 malA1 galK35 rpsL118 thi-1 K. B. Low, CGSC(B.Bachmann & M.Berlin) ME8047 W3110(lambda_papa) F- λ+ lambda_papa IN(rrnD-rrnE)1 W3110 T. Icho ME8048 CA7902 Hfr F HfrH(valS <- uxuAB) thi cya relA1 CA8000 J. Beckwith T. Yokota 29182 ME8049 GP1 Hfr F HfrH(valS <- uxuAB) relA1 thi cya HfrH T. Yokota 29881 ME8050 GP7 F- rpsL31 his-4 proA2 cya thi-1 GP1 AB1157 T. Yokota 29881 ME8053 MS1276 F- galK2 thi-1 thr-1 leuB6 proA2 his-4 argE3 rpsL31 uvrC34 lacY1 mtl-1 xyl-5 ara-14 tsx-33 supE44 thyA hag-208(a.g.type) MS1275 M. Silverman 29905 ME8054 MS726 RecA F- thyA recA hag-726 uvrC34 galU rpsL31 his-4 argE3 MS726 M. Silverman 29905 ME8055 MS1350 NalR F- galU gyrA uvrC34 his-4 rpsL31 argE3 thyA MS1350 Y. Komeda ME8056 MS1032 F- proA2 leuB6 thr-1 thi-1 xyl-5 mtl-1 tsx-33 supE44 recA13 hag-208(a.g.type) lacY1 galK2 rpsL31 argE3 his-4 ara-14 AB2463 M. Silverman 29905 ME8057 MS5016 F- λ+ lambda_T3MS1861 his-4 argE3 thyA rpsL31 galU uvrC34 flaN1861 M. Silverman ME8058 YN2092 F- rpsL nusA1 trpD9778 Y. Nakamura ME8059 GL1 F- pel-21 gal W3102 S. W. Emmons 29785 ME8060 GL1 Su2 F- gal supE pel-21 GL1(=ME8059) S. W. Emmons ME8061 CA8000 Hfr F HfrH(valS <- uxuAB) relA1 thi-1 J. Beckwith ME8062 CA8306 Hfr F HfrH(valS <- uxuAB) relA1 thi-1 DE(cya) J. Beckwith 29918 ME8063 CA8404 Hfr F HfrH(valS <- uxuAB) DE(cya) thi-1 relA1 crp* CA8306(=ME8062) J. Beckwith crp* ; cAMP independent 29730, 29728 ME8064 CA8445 Hfr F HfrH(valS <- uxuAB) thi-1 relA1 DE(crp) DE(cya) CA8404(=ME8063) J. Beckwith 29728 ME8065 W4597 F- galU K-12 T. Fukasawa 30173 ME8066 JC5072 Hfr F HfrKL16-PO45(lysA -> serA) recA67 spc-300 ilv-318 thr-300 A. J. Clark ME8067 WD8014 F- galU mutD aziR rpsL L. Enguist mutD: Genetics 100:7-18(1982) J. Bacteriol. 117:477-487(1974) 35441, 29729 ME8068 JE5504 Hfr F HfrC(purE -> leuS) aroD man pps Y. Nishimura ME8070 YMEL F+ F supF58 mel-1 K-12 T. Icho ME8072 JC411 (ColE1) F- Other mtl-2 xyl-7 malA1 lacY1 or lacZ4 rpsL104 his-1 leuB6 metB1 argG6 supE44 tsx-1 tonA2 gal-6 JC411 D. Helinsky ME8075 RH214,CGSC5827 F- his-204 leu-57 trp-76 argA76 ilv-667 lacZ332 thi-1 xyl-7 mtl-2 malA1 rpsL135 R. K. Herman, CGSC(B.Bachmann & M.Berlin) leu-57, trp-76, his-204, argA76, ilv-667, lacZ332; frameshift mutation 29779 ME8076 JK268,CGSC5564 F- purB58 tna-5 trpA62 trpE61 dadR1 J. Kuhn, CGSC(B.Bachmann & M.Berlin) supplement 40_micro_g/ml Ade ME8077 P678-54 F- gal-6 ara-13 tsx lacY1 azi leuB6 xyl-7 rpsL malA1 mtl-2 tonA2 supE44 thr-1 minB minA thi-1 P678 H. I. Adler -> P. Matsumura "sequence (FASTQ format)
https://ddbj.nig.ac.jp/search/entry/sra-run/DRR058062

Genome information: BFAJ01000001-BFAJ01000077 (77 entries)" 50386, 30184 ME8079 AB1157 F- supE44 thr-1 leuB6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL31 tsx-33 sup-37 AB1133 Howard(flaND) proA2=DE(gpt-proA)DE62 864, 30006, 3338, 3312, 3298, 3252, 866, 865 ME8080 AB1885,CGSC1885 F- thi-1 argE3 his-4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33 supE44 leuB6 thr-1 uvrB5 AB1157 P. Howard, CGSC(B.Bachmann & M.Berlin) 30006, 3338, 3298, 864 ME8081 AB1886,CGSC1886 F- supE44 uvrA6 thr-1 leuB6 thi-1 argE3 his-4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33 AB1157 P. Howard, CGSC(B.Bachmann & M.Berlin) 30006, 861 ME8082 AB1899,CGSC1889 F- rpsL31 ara-14 supE44 tsx-33 mtl-1 galK2 lacY1 proA2 his-4 argE3 thi-1 leuB6 thr-1 lon-1 xyl-5 AB1157(=ME8079) P. Howard, CGSC(B.Bachmann & M.Berlin) 30177 ME8083 AB2470,CGSC2470 F- his-4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33 supE44 thi-1 argE3 leuB6 thr-1 recB21 AB1157(=ME8079) P. Howard, CGSC(B.Bachmann & M.Berlin) 4914, 4648, 30007 ME8084 AB2474,CGSC2474 F- lacY1 rpsL31 tsx-33 supE44 ara-14 xyl-5 mtl-1 argE3 proA2 his-4 lex-1 leuB6 thr-1 thi-1 uvrA6 galK2 AB1886(=ME8081) P. Howard, CGSC(B.Bachmann & M.Berlin) slow grower 29948 ME8085 AB2494,CGSC2494 F- supE44? lex-1 thr-1 metB1 his-4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33? P. Howard, CGSC(B.Bachmann & M.Berlin) slow grower 29948 ME8086 AB3058,CGSC3058 F- thi-1 recC22 thr-1 leuB6 proA2 his-4 argE3 deoB16 ara-16 lacY1 galK2 xyl-5 mtl-1 rpsL31 tsx-33 supE44 CGSC(B.Bachmann & M.Berlin) 29951 ME8087 AB2463 F- recA13 his-4 argE3 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 rpsL31 tsx-33 supE44 proA2 leuB6 thr-1 AB1157(=ME8079) P. Howard -> T. Icho 3338, 865, 864 ME8088 AB2495 F- xyl-5 mtl-1 rpsL31 tsx-33 supE44 trp-35 thyA20 thyR13 thr-1 leuB6 proA2 his-4 argE3 thi-1 lacY1 galK2 ara-14 T. Icho ME8089 EJ862 NalR F- rpsL hag(S+) thyA ara leu trp his argG xyl mtl ilv metA or metB thi gyrA EJ862 Y. Komeda Nal (10_micro_g/ml) ME8090 MS1278 F- lacY1 leuB6 thr-1 hag-208(a,g) mtl-1 proA2 his-4 thi-1 argE3 supE44 tsx-33 rpsL31 galK2 ara-14 xyl-5 thyA MS1277 M. Silverman ME8091 TS241 F- rnc(ts) ME8092 EC-1 F thi DE(lac-pro) EC-0 J. Beckwith 29183 ME8093 YE100 ME8094 EJ710 F- thi trp his argG rpsL leu ara xyl mtl ilv metA or metB gyrA uvrC34 thyA EJ802 Y. Komeda 29735 ME8095 EJ711 F- uvrC34 thyA trp his argG ilv metA or metB thi mtl xyl ara rpsL leu hag-711(a,g) gyrA EJ710(=ME8094) Y. Komeda motile ME8096 F147,CGSC4323 F+ F trp-45 thi-1 pyrD34 tyrA2 thyA33 recA1 galK99 rpsL118 KL262 K. B. Low,CGSC(B.Bachmann & M.Berlin) ME8097 F150 F+ F argG6 lacY1 gal-6 malA1 xyl-7 mtl-2 recA1 leuB6 his-1 metB1 supE44 tsx-1 tonA2 rpsL104 JC1553 D. Fraenkel CGSC4326 delete uvrC 29984 ME8098 F126,CGSC4253 F+ F thi-1 pyrD34 his-68 trp-45 recA1 mtl-2 xyl-7 malA1 galK35 rpsL1181 KL181 K. B. Low, CGSC(B.Bachmann & M.Berlin) ME8099 F125,CGSC4320 F+ F trp-45 thi-1 pyrD34 his-68 recA1 mtl-2 xyl-7 malA1 galK35 rpsL118 KL181 K. B. Low, CGSC(B.Bachmann & M.Berlin) ME8100 594 Srl F- λ+ lambda_d(srl,recA44#13) srl-110 594 H. Ogawa ME8101 EJ772 F- gyrA rpsL sup-126 bfe? lac(Am) tonA(Am)? tsx(Am)? thyA his ilv trp(am) tyr(am) EJ763 Y. Komeda KY1394 EJ759 sup-126 = SuI(ts) ME8102 CSH1 F- rpsL trp thi lacZ CSH(J.H.Miller) ME8103 CSH2 F- rpsL trp lacZ thi CSH(J.H.Miller) ME8104 CSH3 F- lacZ rpsL thi trp CSH(J.H.Miller) ME8105 CSH4 F- trp rpsL thi lacZ CSH(J.H.Miller) ME8106 CSH5 F- trp rpsL thi lacZ CSH(J.H.Miller) ME8107 CSH6 F- lacZ rpsL thi trp CSH(J.H.Miller) ME8108 CSH7 F- lacY(Am) thi rpsL CSH(J.H.Miller) CSH7 = M7047 ME8109 CSH8 F- trp thi rpsL lacZ CSH(J.H.Miller) ME8110 CSH11 F- trp thi rpsL lacZ CSH(J.H.Miller) ME8111 CSH11A F- lacZ thi trp rpsL CSH(J.H.Miller) ME8112 CSH12A F+ F lac(DE(21)) lacI rpsL CSH(J.H.Miller) ME8113 YK4140 F- DE(lac)U169 araD139 flaS4140 thyA gyrA pyrC46 thi rpsL YK410 Y. Komeda 30106 ME8114 F108,CGSC4262 F+ F ara-13 xyl-7 mtl-2 malA1 cys-46 thi-1 leuB6 thr-1 lysA24 supE44 lacY1 tonA2 gal-6 MA50 K.B.Low, CGSC(B.Bachmann & M.Berlin) ME8115 YK4141 F- araD139 thyA flaB4141 pyrC46 DE(lac)U169 rpsL thi gyrA YK410 Y. Komeda 30106 ME8116 CSH26 F- DE(lac-pro) ara thi CSH(J.H.Miller) ME8117 CSH29 F- trpB thi CSH(J.H.Miller) ME8118 CSH32 F- thi trpE CSH(J.H.Miller) ME8119 CSH33,WD5017 F+ F thi CSH(J.H.Miller) ME8120 CSH40 F+ F DE(lac-pro) thi CSH(J.H.Miller) ME8122 CSH28 F+ F his trp supF DE(lac-pro) rpsL pyrF thi CSH(J.H.Miller) ME8123 CSH38 F+ F thi supE DE(lac-pro) CSH(J.H.Miller) ME8124 CSH17,H145 F+ F thi supE DE(lac-pro) CSH(J.H.Miller) ME8125 CSH18 F+ F DE(lac-pro) supE thi CSH(J.H.Miller) CSH18 = H125 ME8126 CSH19,H138 F+ F DE(lac-pro) thi supE CSH(J.H.Miller) ME8127 CSH13,H120 F+ F supE DE(lac-pro) thi CSH(J.H.Miller) ME8128 CSH14,H111 F+ F thi supE DE(lac-pro) CSH(J.H.Miller) ME8129 CSH15,H119 F+ F thi DE(lac-pro) supE CSH(J.H.Miller) ME8130 CSH20B F+ F DE(lac-pro) supE thi CSH(J.H.Miller) ME8132 CSH35,E7101 F+ F DE(lac-pro) supE thi CSH(J.H.Miller) ME8133 D33 Hfr F HfrC lacIQ met J. R. Sadler 30038 ME8134 D1203 F+ F supE44 endA recA13 hsdM hsdR thi-1 leuB6 proA2 gal lacZ4 HB101(=ME8568) J. R. Sadler 30038 ME8135 D1204 F+ F lacZ4 thi-1 leuB6 hsdR hsdM recA13 supE44 endA rpsL proA2 gal HB101 J. R. Sadler 30038 ME8136 D1210 F- lacIQ proA2 leuB6 thi-1 hsdR hsdM supE44? endA? rpsL recA56 gal HB101(=ME8568) J. R. Sadler 30038 ME8137 CSH24,EC-0 F+ F supE DE(lac-pro) thi J. Beckwith, CSH(J.H.Miller) ME8138 CSH44,BMH479 F- λ+ lambda_cI857 St68 h80 lambda_cI857 St68 h80 d-lac+ tonA thi DE(lac) Icho & Komeda, Tokyo University, CSH(J.H.Miller) 30192 ME8139 CSH45 F- λ+ lambda_cI857S7 trpR thi DE(lac) CSH(J.H.Miller) ME8140 CSH46,M96 F- λ+ lambda_cI857 St68 h80 thi ara DE(lac-pro) CSH(J.H.Miller) ME8141 CSH54,X7150 F- thi his rpsL trp supF DE(lac-pro) mal pyrF CSH(J.H.Miller) ME8142 CSH55,X7026N F- DE(lac-pro) thi supE gyrA X7026 CSH(J.H.Miller) ME8143 CSH56,X68C F- supD ara DE(lac-pro) thi gyrA CSH(J.H.Miller) ME8144 CSH57B F- lac mtl gal tsx thi ara xyl rpsL leu purE trp his metA or B,ilvA argG CSH(J.H.Miller) ME8145 CSH41 F+ F DE(lac-pro) galE thi Y. Komeda, CSH(J.H.Miller) ME8146 CSH59,X181a F- thi pyrC trp rpsL CSH(J.H.Miller) ME8147 CSH60,RA-2 Hfr F sup CSH(J.H.Miller) ME8148 CSH71 Hfr F HfrH(valS <- uxuAB) DE(gal-att80_lambda_bio uvrB) thi CSH(J.H.Miller) CSH71=RW361 ME8149 CSH73 Hfr F HfrH(valS <- uxuAB) thi DE(lac) DE(ara-leu) CSH(J.H.Miller) ME8150 CSH53 (=X8632) F- φ80- phi_80 d(lacI-Z+) thi ara DE(lac-pro) rpsL CSH(J.H.Miller) ME8151 CSH51 F- φ80- phi_80d-lac+ rpsL thi DE(lac-pro) ara CSH(J.H.Miller) CSH51 = X7700 ME8152 CSH68 Hfr F Hfr6(lac <- proC) malB met mtl CSH(J.H.Miller) CSH68 = Hfr6 ME8153 CSH64 Hfr F HfrKL14(tolC <- cca) thi CSH(J.H.Miller) CSH64 = KL14 ME8154 CSH62 Hfr F HfrH(valS <- uxuAB) thi CSH(J.H.Miller) CSH62 = CA8000 ME8155 CSH47A Hfr F HfrKL25(pyrE <- dnaA) sup CSH(J.H.Miller) CSH47A = KL25 ME8156 CSH70 Hfr F HfrP4X(argF -> lac) argE metB thi CSH(J.H.Miller) ME8157 CSH69 Hfr F HfrKL99(fabA <- pyrC) thi CSH(J.H.Miller) ME8158 CSH34,E7089 F+ F thi DE(lac-pro) supE CSH(J.H.Miller) ME8159 CSH21 F+ F supE gyrA thi DE(lac-pro) CSH(J.H.Miller) ME8160 CSH20A F supE thi DE(lac-pro) CSH(J.H.Miller) ME8161 CSH66 F- λ+ lambda_cI857 S7 p-lac5(I-Z+Y-) DE(lac) thi CSH(J.H.Miller) CSH66 = M7133 ME8162 CSH67 Hfr F HfrR5(leuA -> tonA) λ+ thi (lambda)+ lac gal xyl mtl malA CSH(J.H.Miller) "CSH67 = R5

Genome information: BFAK01000001-BFAK01000087 (87 entries)" 50386 ME8163 CSH74 Hfr F HfrKL16(thyA -> serA) thi CSH(J.H.Miller) CSH74 = K46, KL16 ME8164 CSH49 F- φ80- phi_80d-lacI phi_80h p-trp thi rpsL ara DE(lac-pro) DE(trp)A-D CSH(J.H.Miller) ME8165 CSH72 F+ F CSH(J.H.Miller) ME8261 LA5641 F- ptsF25 flbB5301 DE(argF-lac)U169 rpsL1150 araD139 gyrA glpR DE(glpT-glpA)593 mgl-500::Tn10 relA deoC1 LA5606 TL45 30060 ME8262 LA5642 F- gyrA araD139 DE(argF-lac)U169 rpsL1150 flbB5301 deoC1 ptsF25 relA zef-704::Tn10 DE(glpT-glpA)593 glpR LA5564 TL45 Co-td: cdd : 20% gat : 40% 30044 ME8263 LA5651 F- λ+ (lambda)+ zeg-722::Tn10 gyrA thr-1 leuB6 lacY1 phoA14 rpsL114 malA1 metB1 his gatA mglB550 DL4 AW550 Co-td; gyrA-20% mgl - 7% 30060 ME8264 LA5652 F- cir::Mud(Ap,lac) thi tsx DE(proAB-lac) malPQ::Tn10 aroB HS203 H1300 30060 ME8265 LA5579 F- flbB5301 zee-700::Tn10 fpk araD139 DE(argF-lac)U169 rpsL1150 deoC1 ptsF25 relA DE(glpT-glpA)593 gyrA glpR LA5531 TL45 Co-td fpk/zee-700::Tn10 : 30% cdd/zee-700::Tn10 : 60% 30044 ME8266 Derival MC4100 FlbB+ cir::Tn10 F- araD139 relA ptsF25 deoC1 rpsL1150 DE(argF-lac)U169 cir::Tn10 Co-td fpk/cir::Tn10 : 50% cdd/cir::Tn10 : 30% 3283 ME8267 LS5394 F- trpA61 (lambda)- dadR fadR::Tn5 purB58 trpE D. N. William supplement 40_micro_g/ml Ade ME8268 SY593 F- srl::Tn10 recA56 S. Yasuda SY593 = W3110 recA56 srl::Tn10 ME8269 JC10289 F- tsx-33 DE(srl-recA)306::Tn10 supE44 thr-1 leuB6 proA2 his-4 argE3 thi-1 mtl-1 ara-14 lacY1 galK2 xyl-5 rpsL31 29761 ME8270 ED1032 Hfr F HfrPO201 TP3 DE(gpt-lac)5 relA1 rpsE2123 thi-1 supE44 TP3 = lac transpored to Hfr transfer terminus 29897 ME8271 MCL30 Hfr F HfrPO45(thyA -> serA) thi-1 DE(srl-recA)306::Tn10 30058 ME8272 MCL31 Hfr F HfrPO201 TP3(=lac transposed to Hfr transfer terminus) DE(gpt-lac)5 relA1 rpsE2123 thi-1 supE44 DE(srl-recA)306::Tn10 30058 ME8273 BW280,CGSC6097 Hfr F HfrPO45 of HfrKL16(thyA -> serA) spoT1 thi-1 (lambda)- zfe-208::Tn10 nadB7 ung-1 relA1 CGSC(B.Bachmann & M.Berlin) 29754, 30087, 30097 ME8275 D6434 F- gyrA rpoB supP argE(Am) DE(lac-pro)XIII supP=Su6+ 30027, 30052 ME8276 AW1013 F- rpsL1156 relA1 tsx::Tn5 araD139 thi DE(argF-lac)U169 ptsF25 deoC1 flbB5301 Andrew Wright->Wanner->Nishimura Pedigree : MC4100 AW1013 = 2054 30104 ME8277 BW1247 F- phoR68 DE(lac)169 phoM451 zaa::Tn10 thi rpsL proC::Tn5 V1283 phoM TcR 30104 ME8278 DB6960 ilv::Tn10 thi pro hsr sup David Botstein->Wanner->Nishimura strain 294 derived hsr(=hsdR) DB6960 = V436 30104 ME8279 CH1524 trpA36 zib-137::Tn10 lysA xyl-4 ilvD130 argH1 Tn10 is inserted distal to mtl with respect to xyl. 30059, 3187 ME8280 CH1525 (inserted between mtl and xyl) zia-138::Tn10 argH1 ilvD130 xyl-4 lysA trpA36 30059 ME8281 LCB482 mtl-10 rpsL175 supE44 tonA21 thr-1 leuB6 thi-1 lct-2 lacY1 Coli Genetic Stock Center Strain 5186 30059 ME8282 CAG1128 galK zai::Tn10 proC leu thr supE tonA lacY thi EJ403 CAG1125 Pro- TcR P1(EJ403) to CAG1125 select tetR(Tn10) screen pro- 30074 ME8283 CAG1138 F- thr lacY lon zai::Tn10 galK supE thi leu tonA SG4044 CAG1128 lon (mucoid) Pro+ 30074 ME8284 JT16 trpE9829 pyrF cysB216 M. D. Yudkin 30103 ME8285 SK1105 leu pyrF::Tn5 trpE5 thr MG1300 MV10 pyrF::Tn5 KmR MV10(Kahn et al.1979) 30062, 30076 ME8286 MC4100 F- flbB5301 tonA21 rpsL150 DE(argF-lac)U169 araD139 thiA deoC1 ptsF25 relA1 Schultz et. al.(1982) 30061, 29997 ME8287 8 Hfr F HfrC(purE -> lip) λ+ phoA8 glpD3 glpR2 relA1 tonA22 (lambda)+ Hayashi et al(1964) glpD strains should be maintained on media containing G3P or glycerol (LB tryptone, antibiotic medium 3 etc) only in the presence of 10mM glucose to avoid groth stasis. 30061, 29997 ME8288 SH205 Hfr F HfrC(purE -> lip) λ+ (lambda)+ phoA8 glpD3 glpR2 relA1 tonA22 zah-735::Tn10 DE(argF-lac)U169 glpD strains should be maintained on media containing G3P or glycerol (LB, tryptone, antibiotic medium 3 etc) only in the presence of 10mM glucose to avoid growth stasis 29997, 30061 ME8289 SH206 Hfr F HfrC(purE -> lip) (lambda)+ phoA8 glpD3 glpR2 relA1 tonA22 zai-737::Tn10 DE(argF-lac)U169 glpD strains should be maintained on medium containing G3P or glycerol(LB, trypton, antibiotic medium 3 etc) only in the presence of 10mM glucose to avoid growth stasis. 30061, 29997 ME8290 BW9116 Hfr F HfrKL16 zdh-201::Tn10 thi-1 DE(xthA-pncA)90 relA1 spoT1? zdh-201::Tn10; near ackB, co-transduced gap pyk 30109, 30020, 29723 ME8291 DC335 F- aroC his zfa::Tn10 str Co-td ackA/zfa::Tn10 : 16% pta /zfa::Tn10 : 68% 29723, 30109, 30020 ME8292 DC356 F- zgb-6::Tn10 fadR adhC Co-td ; fda pgk 29723, 30109, 30020 ME8293 DC372 F- adhC fadR srl::Tn10 srl::Tn10 is co-transduced with eno 29723, 30020, 30109 ME8294 ET1214 F- rpsL9 zig-219::Tn10 thi-1 lacY1 gal-6 malA1 xyl-7 mtl-2 tonA2 tsx-68 (lambda)- supE44 lambda_R zig-219::Tn10 = zig-2::Tn10 Co-td tpi/zig-219::Tn10 : 44% glnA/zig-219::Tn10 : 85% rha/zig-219::Tn10 : 20% pfkA 30020, 30109, 29723 ME8295 JW375 F- Fru supE44 zhb-511::Tn10 metB relA nalA Co-td mdh Fru: Genetically uncharacterized mutation preventing growth on fructose 30020, 29723, 30109 ME8298 TL212 F- malTC-1 DE(malE)444 zce-726::Tn10 araD139 rpsL150 DE(argF-lac)U169 deoC1 relA1 thiA ptsF25 flbB5301 Co-td ptsG/zce-726::Tn10 : 80% 29723, 30109, 30020 ME8299 UM119 F- katF2 thr-1 leuB6 thi-1 argE3 his-4 proA2 lacY1 galK2 ara-14 xyl-5 mtl-1 rpsL tsx-33 supE44 katE1 pfkB::Tn10 864, 30063, 865 ME8300 UM120 Hfr F HfrH katE12::Tn10 thi 30019 ME8301 UM122 Hfr F HfrH katF13::Tn10 thi 30019 ME8302 UM156 F- azi-6 rpsL109 tonA23 tsx-67 supE44 malA38 xthA katF3 kat-3b cysI::Tn10 lacY1 thi-1 met-160 ilvA681 argG77 his-208 trpE28 purE42 proC83 leuB6 ara-14 galK2 xyl-5 mtl-1 supplement 40_micro_g/ml Ade 30019 ME8303 UM197 F- purE42 leuB6 proC83 trpE38 his-208 argG77 ilvA681 metA160 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 azi-6 rpsL109 tonA23 tsx-67 supE44 malA38 xthA katG17::Tn10 29862, 865, 864 ME8304 CS1129 F+ F ompR151 recA lac 29826 ME8305 RK4784 F- rpsL150 DE(argF-lac)U169 araD139 recA1 deoC1 flbB5301 DE(btuB) metE70 non gyrA219 thi zeh::Tn10 DE(ompC) 29826 ME8306 RK4786 F- DE(btuB) fibB5301 deoC1 rpsL150 recA1 araD139 metE70 non gyrA219 ptsF25 thi ompF::Tn5 DE(argF-lac)U169 29826 ME8307 RK4788 F- DE(argF-lac)U169 araD139 recA1 rpsL150 fibB5301 deoC1 thi ptsF25 gyrA219 non metE70 DE(btuB) DE(ompA) zcb::Tn10 29826 ME8308 RK4792 F- malP::Tn10 ompR151 DE(btuB) metE70 non gyrA219 thi deoC1 flbB5301 rpsL150 relA1 araD139 DE(argF-lac)U169 29826 ME8311 IQ399 F- argH thi-1 lacY1 or lacZ4 xyl-1 mtl-2 rpsL8,9 or 14 (lambda)- hisS210 supE44? zfe-99::Tn5 glyA6 relA1? hisS210 = Ts 29754, 30097, 30087 ME8312 IQ417 F- deoC1 zfe-208::Tn10 glyA6 DE(argF-lac)U169 araD139 relA1 rpsL150 flbB5301 tonA21 thi ptsF25 30087, 30097, 30067, 29754 ME8313 205(#17) Hfr F HfrC metB proA3 metD88 lac-3 rnh::Tn3 relA1 tsx-76 KH1192 30018, 29958 ME8314 112(#2) Hfr F HfrC(purE -> lip) metB proA3 metD88 lac-3 tsx-76 relA1 zaf::Tn3 KH1192 30018, 29958 ME8315 BH2008 F- ilvC::Tn5 argH1 metE46 rpsL hsdR galK galU araD139? 29857 ME8316 A95 Hfr F HfrC(purE -> lip) ilv fip-1 zie-1::Tn5 29857 ME8317 A179 Hfr F HfrC(purE -> lip) λ+ (lambda)+ fip::Kan fip(=trxA) 29857 ME8318 PC2zjj-202::Tn10 F- dnaC2 thy leu str zjj-202::Tn10 29860, 29978 ME8319 PC5zib-501::Tn10 F- leu zib-501::Tn10 dnaA thy str 29978, 29860 ME8320 UT5028 F+ serB thi zji-1::Tn10 29823 ME8321 SS3012 F- (lambda)- P1S zei-298::Tn10 ompF::Tn5 met-90 azi-9 rpsL his-85 trpR trpA lacZ lacI22 gyrA pro-48 DE(ompC178) 29823 ME8323 IT1528 F- ilvY864::Tn10 gal-3 DE(cya) rho-15 29850 ME8324 IT1534 F- zjb::Tn10 gal-3 ssb-113 29850 ME8326 AI372 F- trp(am) tyr(am) tonA(Am) tsx(Am) btuB sup-126 lac(Am) ilv::Tn10 uncA401 his 30068 ME8327 AI102 F- rbs-102::Tn10 30068 ME8328 EJ1396 H1-i-vh2 H2-enx rhl zcg-2::Tn10 thr leu trp his arg thi lac str 30082 ME8329 PT214 Hfr F HfrH dgk-6 mdoB::Tn10 30022 ME8330 BN904 F- thi-1 rpsL129 relA1? (lambda)- galK30 fur::Tn5(B20) gltA6 pyrD36 supE44 29871 ME8331 BN905 F- purB15 lip-9 fur::Tn5(B20) thi-1 proA2 his-4 mtl-1 xyl-5 galK2 lacY1 strA35 (lambda)- supE44? 29871 ME8332 BN900 F- nagA1 fur::Tn5(B20) strA155 rpoB352 metB1 argH1 29871 ME8333 JF496 Hfr F HfrR1(mtl<-ilv) rbs-4 metB1 spoT1 bglR13 asnA31 relA1 nagB2 asnB50::Tn5 ubiF411 35194, 30027 ME8334 CBK001 F- thyA DE(lacA-argF)? pheA::Tn5 lambda_- W3110 ThyA 30087, 29759 ME8335 CBK012 F- lambda_- DE(lacA-argF)? thyA leu::Tn5 W3110 ThyA 29759, 30087 ME8336 CBK017 F- thyA DE(lacA-argF)? (lambda)- argE::Tn5 W3110 ThyA 29759, 30087 ME8337 CBK040 F- thyA metE::Tn5 lambda_- DE(lacA-argF)? W3110 ThyA 29759, 30087 ME8339 CBK130 F- lambda_- thyA proAB::Tn5 DE(lacA-argF)? W3110 ThyA 30087, 29759 ME8340 CBK140 F- thyA lysA::Tn5 (lambda)- DE(lacA-argF)? W3110 ThyA 29759, 30087 ME8341 CBK236 F- (lambda)- DE(lacA-argF)? hisG::Tn5 thyA W3110 ThyA 30087, 29759 ME8342 CBK252 F- thyA ilvC711::Tn5 lambda_- DE(lacA-argF)? W3110 ThyA 29759, 30087 ME8343 CBK044 F- DE(lacA-argF)? thyA thrA::Tn5 (lambda)- W3110 ThyA 30087, 29759 ME8344 CBK031 F- DE(lacA-argF)? thyA carA or B::Tn5 (lambda)- W3110 ThyA 29759, 30087 ME8345 CBK076 F- lambda_- thyA proAB::Tn5 DE(lacA-argF)? W3110 ThyA 29759, 30087 ME8346 CBK026 F- DE(lacA-argF)? thyA purE::Tn5 (lambda)- W3110 ThyA 30087, 29759 ME8347 CBK005 F- DE(lacA-argF) (lambda)- pyrC or D::Tn5 thyA W3110 ThyA 30087, 29759 ME8348 CBK070 F- DE(lacA-argF)? (lambda)- trpA or B::Tn5 thyA W3110 ThyA 29759, 30087 ME8349 CBK142 F- (lambda)- DE(lacA-argF)? thyA purF::Tn5 W3110 ThyA 29759, 30087 ME8350 CBK110 F- dapA or E::Tn5 thyA DE(lacA-argF) (lambda)- W3110 ThyA 29759, 30087 ME8351 CBK225 F- DE(lacA-argF) thyA purCG or I::Tn5 (lambda)- W3110 ThyA 30087, 29759 ME8352 CBK081#1,#2 F- thyA glyA::Tn5 lambda_- W3110 ThyA 30087, 29759 ME8353 CBK096 F- thyA cysHD or C::Tn5 (lambda)- DE(lacA-argF) W3110 ThyA 30087, 29759 ME8354 CBK141 F- DE(lacA-argF) thyA aspB::Tn5 (lambda)- W3110 ThyA aspB(=gltB) 30087, 29759 ME8355 CBK286 F- DE(lacA-argF) (lambda)- cysE::Tn5 thyA W3110 ThyA 30087, 29759 ME8356 CBK046 F- lambda_- metJBLF or M::Tn5 DE(lacA-argF)? thyA W3110 ThyA 30087, 29759 ME8361 CBK018 F- (lambda)- DE(lacA-argF)? thyA serB::Tn5 W3110 ThyA 29759, 30087 ME8362 AC109 glpR(?) glpK phoA8 ilv ugpA704::Tn10 29859 ME8363 HR221 Hfr F HfrKL16(thyA -> serA) gyrA zhf::Tn10 ilvA::Tn5 DE(glpTA) 29859 ME8364 HR203 Hfr F HfrKL16(thyA -> serA) zhf::Tn10 29859 ME8365 HW21 Hfr F HfrH(valS <- uxuAB) glpR zhe::Tn10 glpD (lambda)- 30064 ME8366 HW60 Hfr F HfrC(purE -> lip) glpD hemA30 zch::Tn10 glpR hemA30: require 40_micro_g/ml of delta-aminolevulinic acid 30064 ME8367 GS606 thi zgb224::Tn10 glyA::Mucts serA25 lambda_S pheA905 29856 ME8368 JC8947(=CH1221) F- galK2 argE3 rpsL31 tsx-33 supE44 sup-37 umuC::Tn5 thr-1 leuB6 thi-1 lacY1 ara-14 xyl-5 mtl-1 proA2 his-4 29825 ME8369 CH1418 lambda_R (lambda)- supE63 thi-1 his-68 e14-1272::Tn10 tyrA2 trp-45 purB51 lacY1 gal-6 mtl-2 xyl-7 malA1 str-125 tonA2 tsx-70 29825 ME8370 CH1407 F- lacY1 galK2 str-20 tsx-57 tfr-5 mtl-1 xyl-5 e14-1272::Tn10 fabD1 thi-1 gltA5 ara-14 fabD1 = TS 29825 ME8371 TG11 Hfr F HfrPO3 of HfrP4X(argF -> lac ) metB1 uxaA1 tdc::Tn5 relA1 30069 ME8372 AE1122 Hfr argG6 metB1 zeb-1::Tn10 thyA rpsL104 Co-td zwf/zeb-1::Tn10 : 58% 29853 ME8373 CK1640 F- mtl DE(argF-lac)U169 araD139 relA1 rpsL150 thi zia::Tn10 purE ptsF25 tonA21 deoC1 flbB5301 29853 ME8374 DB821 F- thi nusA1 ptsF25 tonA21 deoC1 flbB5301 relA1 rpsL150 zgj::Tn10 DE(argF-lac)U169 araD139 29853 ME8377 RS3338 F- fadL71::Tn10 fadR 29853 ME8378 SY798 F- Other hfl-1 DE(lac)M445 lac-1 his argE rpsL mtl xyl DE(recA-srl) zje::Tn10 Co-td ompC/zje::Tn10 : 43% hfl /zje::Tn10 : 10% 29853 ME8379 SY657 F- mtl-1 supE44 argE3 his-4 proA2 leuB6 tsx-33 thr-1 xyl-5 galK2 lacY1 thi-1 sup-37 zai::Tn10 ara-14 str-31 Co-td proC/zai::Tn10 : 18% 29807 ME8380 SY844.2 F+ F DE(lac-pro)XIII argE(Am) nalA rifR leu::Tn5 DE(lac-pro)XIII = DE(gpt-lac)5 29807 ME8381 SY929 F- metD thr-1 leuB6 proA2 his-4 argE3 str-31 ara-14 zae-13::Tn10 drpA1 lacY1 galK2 xyl-5 mtl-1 tsx-33 supE44 sup-37 drpA = TS 29807 ME8383 GW2730 argE3 leu-6 thr-1 his-4 DE(lac)U169 rpsL31 galK2 sulA11 tif-1 ilv lexA71::Tn5 tif-1 = recA441 sulA11 = sfiA ilv = Ts 29827 ME8384 GW5229 lon-100 tsx::Tn10 29827 ME8385 SJ16 F- (lambda)- gyrA216 relA1 metB zad-220::Tn10 panD2 lambda_R 29861 ME8386 JFC124 F+ F Other DE(lac-pro) supL2 asuE9 zce-464::Tn10 purB51 gyrA DE(tonB-trpAB) argE(UAG) his 29854, 29870 ME8387 JFC152 F+ argE(UAG) his DE(tonB-trpAB) gyrA DE(lac-pro) zie-530::Tn5 ilvD188(UAA) 29854, 29870 ME8388 JFC206 F+ F pit-10 DE(tonB-trpAB) supL2 DE(lac-pro) gyrA argE(UAG) his zhg-525::Tn10 29870, 29854 ME8389 JFC208 F+ F zhg-458::Tn10 DE(tonB-trpAB) his argE(UAG) gyrA DE(lac-pro) supL2 pit-10 29870, 29854 ME8390 L-48 F- gltA5 fadD85 lct-1 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL20 tsx-57 tfr-5 supE44 29870, 29854 ME8391 MK60L/IQ1 F+ F supL2 DE(tonB-trpAB) his argE(UAG) gyrA DE(lac-pro) 29854, 29870 ME8393 RS3245 F- traA62 dadR1 trpE617 tna-5 be-120::Tn10 29832 ME8394 RS3263 F+ fadR::Tn5 bef-108::Tn10 29832 ME8395 SBS821 F- Other Mu+ ? appA1 pyrD34 trp-45 his-68 thyA25 thi deoR33 galK35 xyl-7 mtl-2 malA1 rpsL118 lambda_R (lambda)- putA::Tn5 29836 ME8396 SBS1088 Hfr F HfrC(purE -> lip) zcc::Tn10 relA pit-10 tonA22 rpsL appA1 Co-td appA/zcc::Tn10 : 60% ME8397 LS5786 F+ thi-1 metE68 relA fadR::Tn5 fadAB::Tn10 30032 ME8398 LS6745 F- metB lacY fad-5 supE supF zif::Tn10 trpR galK hsdR Co-td fadAB/zif::Tn10 : 95% 30032 ME8399 TS T3 F- tonA21 malT::Tn10 flbB5301 ptsF25 thiA relA1 deoC1 DE(argF-lac)U169 araD139 rpsL150 30021 ME8400 TL84 Hfr F HfrC(purE -> lip) λ+ phoA8 glpD3 glpR2 spoT1 relA1 pit-10 fhuA22 ompF627 fadL701 glpKi plsB26 plsX50 zjb-750::Tn10 (lambda)+ 30021 ME8401 TL112 Hfr F HfrC λ+ plsB26 phoA8 glpD3 glpR2 relA1 spoT1 pit-10 fhuA22 ompF627 fadL701 glpKi gyrA zce-726::Tn10 (lambda)+ Co-td ptsG/zce-726::Tn10 : 80% 30021 ME8402 TL247 F+ spoT1 zce-727::Tn10 ptsG21 thi-1 relA1 30021 ME8403 TL270 F- λ+ mtl-1 (lambda)+ zce-727::Tn10 supE44 tfr-5 tsx-57 thi-1 lct-1 ara-14 lacY1 galK2 xyl-5 fabD89 gltA5 rpsL20 30021 ME8404 TL329 F+ (fabF200?) spoT1 relA1 thi-1 ptsG21 zcf-229::Tn10 30021 ME8405 TL323 Hfr F HfrC(purE -> lip) λ+ fadL701 relA1 spoT1 pit-10 fhuA22 ompF627 glpR2 glpKi malE::Tn5-7 zce-727::Tn10 (lambda)+ phoA8 glpD3 30021 ME8406 TL324 Hfr F HfrC(purE -> lip) zce-727::Tn10 phoA8 glpD3 glpR2 relA1 spoT1 pit-10 fhuA22 ompF627 fadL701 glpKi plsX50 malE::Tn5-7 30021 ME8407 TL325 Hfr F HfrC(purE -> lip) λ+ (lambda)+ glpR2 relA1 spoT1 pit-10 fhuA22 ompF627 fadL701 glpKi plsB26 malE::Tn5-7 zce-727::Tn10 glpD3 phoA8 30021 ME8408 P4X8 Tsx::Tn5 Hfr F HfrP4X(argF -> lac) tsx::Tn5 (lambda)- metB ME8409 610 zaf-2::Tn3 Hfr F HfrIOR-3 leu zaf-2::Tn3(ApR) (lambda)- thy lacIZYA inverted 30018, 29958 ME8410 KN673 thr his trpE9829(Am) tyrA sup-126 dnaG2903 tolC663 thy zgh-641::Tn10 ilv All media are to be supplemented with 50_micro_g/ml thymine. 29863 ME8411 KN696 F- gal zgh-696::Tn10 thyA thr leu his met trp thi lac mal xyl mtl ara tonA rpsL azi All media are to be supplemented with 50_micro_g thymine/ml. 29863 ME8412 GW2100 F- leuB6 his-4 thi-1 argE3 lacY1 galK2 ara-14 xyl-5 mtl-1 tsx-33 rpsL31 umuC122::Tn5 thr-1 proA2 sup-37 supE44 30055 ME8413 GC2418 Hfr F HfrKL16(thyA -> serA) srl::Tn10 psd rif-1 ME8414 DM49 ara lexA3 thr pro leu his arg gal lac xyl str S. Casaregora 29882 ME8415 GC2597 leu his lac gal malB srl::Tn10 sfiA::Tn5 pyrD thr str sfiC 30055 ME8416 GC4276 argE lac thi xyl ara rpsL mtl tsx his thr sfiB114 ME8417 GC4670 lacY leu thr lon::Tn10 ME8418 NK6051,CGSC6186 Hfr thi DE(gpt-lac) spoT relA purE::Tn10 CGSC(B.Bachmann & M.Berlin) require Ade (40_micro_g/ml) and Vitamine B1 29821 ME8419 K797,CGSC6456 F- aroA phoR::Tn10 purE his ilv metB lacY xyl rpsL cycA cycB? tsx tonA? CGSC(B.Bachmann & M.Berlin) 29821 ME8420 ECL510 F- araD139 DE(glpD)102 ptsF25 DE(frd)101 zjd::Tn10 thi sdh-9 flbB relA rpsL DE(lac)U169 Co-td frd/zjd::Tn10 : 70% 29858 ME8421 ECL512 F- araD139 fnr ptsF25 flbB relA rpsL DE(glpD)102 zci::Tn10 DE(lac)U169 thi sdh-9 29858 ME8422 ECL289 Hfr F HfrC(purE -> fep) λ+ T2R (lambda)+ tonA22 relA1 argA::Tn10 eno 30093 ME8423 IQ85 F- flbB ptsF25 zhc::Tn10 rpsL deoC1 secY spc tonA21 araD139 thi DE(lac)U169 relA between str and spc secY = ts-24 30086 ME8424 BD1467 Hfr F HfrPO45 λ+ tyrA::Tn10 ung-1 nadB (lambda)+ relA1 thi use strain name for publication 29864 ME8425 BW313 F+ spoT1 ung-1 relA1 dut-1 Can make phage _lambda_ having uracil. Assign strain name BW313 when describing this strain in a manuscript. F+=KL16 derivative ME8426 SG1501 F- DE(lac) DE(lon)100 non-3::DE(Tn10) araD139 rpsL ilv::Tn5 same as SG20150(=ME8427) but DE(Tet) ME8427 SG20150 F- non3::Tn10 araD139 rpsL ilv::Tn5 DE(lac) DE(lon)100 ME8428 YYC201 Hfr F HfrC(purE -> fep) pfl-1 DE(aroP-aceEF) (lambda)- zbi::Tn10 pps-1 YYC187(lambda)- = YYC201 30078 ME8429 CY377 zac::Tn10 nalA Fru metB relA DE(aroP-aceEF) leu Fru; Genetically uncharacterized mutation preventing growth on fluctose. 30033 ME8430 EL33 suO uvrA6 rpsL200 thi malE57::Tn5 DE(kdp-phr)214 29855, 3242 ME8432 X2844,CGSC6683 F- (lambda)- tsx-462::Tn10 CGSC(B.Bachmann & M.Berlin) ME8433 YYC188,CGSC6776 Hfr F HfrC(purE -> fep) λ+ lambda_cI857 pps-4 pfl-1 poxB11 zbi-927::Tn10 thi-1 ton-54? DE(aroP-aceF)73 azi-14? lacZ608(Am) rpsL104 CGSC(B.Bachmann & M.Berlin) This strain must be grown at a temperature below 33C. 30078 ME8434 LCB274,CGSC6544 F- leuB6 thr-1 lacY1 zbj-274::Tn9 rpsL thi-1 CGSC(B.Bachmann & M.Berlin) ME8435 RW1230,CGSC6392 F- mtl-1 (lambda)- supE44? DE(gpt-proA)62 zbj-1230::Tn10 hisG4 thi-1 lacY1 galK2 xyl-5 CGSC(B.Bachmann & M.Berlin) Co-td aroA/zbj-1230::Tn10 : 88% ME8437 NK6702,CGSC6190 F- (lambda)- IN(rrnD-rrnE)1 man-6::Tn9 CGSC(B.Bachmann & M.Berlin) ME8438 UT152#1,#2,#3,CGSC6583 F- zdg::Tn10 or zdf::Tn10 ara nalA DE(lac-pro) rifR argE(Am) thi tyrS565 CGSC(B.Bachmann & M.Berlin) Co-td: tyrS(Ts) ME8439 DF949,CGSC6792 Hfr F HfrC(purE -> lip) ompF627(T2R) tonA22 zdh-925::Tn10 gnd-1 relA1 pit-10 spoT1 CGSC(B.Bachmann & M.Berlin) 30036 ME8440 DC369,CGSC6355 F+? zdj-225::Tn10 fadR16 butD12(=atoC) mel-1 supF58 CGSC(B.Bachmann & M.Berlin) Co-td gap /zdj-225::Tn10 : 58% fadD/zdj-225::Tn10 : 42% ptsM/zdj-225::Tn10 : 25% ME8441 JW381,CGSC6095 F- mtl-1 thi-1 (lambda)- zed-508::Tn10 supD60 his-4 rpsL136 xyl-5 metF159 eda-50 DE(gal-att_lambda)99 tsx-78 lacY1 tonA31 leuB6 ara-14 thr-1 CGSC(B.Bachmann & M.Berlin) Co-td supD/zed-508::Tn10 : 89% ME8442 N3024,CGSC6658 F- uvrC279::Tn10 (lambda)- IN(rrnD-rrE)1 CGSC(B.Bachmann & M.Berlin) ME8443 DF264,CGSC6381 Hfr F HfrC(purE -> fep) zgd-210::Tn10 relA1 pit-10 tonA22 spoT1 T2R pgk-2 CGSC(B.Bachmann & M.Berlin) ME8444 NK6027(=CGSC6179) Hfr F HfrH(valS <- uxuAB) spoT1 (lambda)- DE(gpt-lac)5 relA1 metC162::Tn10 thi-1 ME8445 S1228,CGSC6432 F- rpsL272 thr-36 trp-88 his-218 lysA35 ilv-688 metF178 lac-97 xyl-19 tsx-460 zhj-429::Tn10 (lambda)- CGSC(B.Bachmann & M.Berlin) Co-td; bisC: 30% xyl : 50% 30099 ME8446 A238 Hfr F HfrC(purE -> lip) λ+ zie-1::Tn5 trxB zbj-1230::Tn10 (lambda)+ supD trxA(Am) 29831 ME8447 KD1996 F- pro thi ton polA12 recQ61::Tn3 ilvD145 trpC3 mtl-1 malA1 ara-9 galK2 lac-114 rpsL 861, 29837 ME8448 KD2157 F- ara-9 malA1 mtl-1 thyR thyA::Tn5 thi pro trpC3 his-4 ilvD145 metE46 ton rpsL lac-114 galK2 29837 ME8450 RK4342,CGSC6402 F- his218 pro-3 DE(lac)6 entA403 zbc-601::Tn10 supE44(=glnV44) rpsL109 xyl-5 or xyl-7 ilvC7 metB1 (lambda)- CGSC(B.Bachmann & M.Berlin) zbc-601::Tn10 is close to entA. Deletion 6 is the allele lac72 of Lederberg strain W4032,a deletion of the entire lac operon. ME8451 SK2257,CGSC6422 F- deoC1? rpsL120 thyA6 zbe-280::Tn10 CGSC(B.Bachmann & M.Berlin) zbe-280::Tn10 cotransduces 76% with leuS and also cotransduces with rna. 29837 ME8452 JW1071 Hfr F HfrPO1 of HfrH(valS <- uxuAB) spoT1 (lambda)- relA1 lacZ125 trp-49 zbf-507::Tn10 Co-td supE/zbf-507::Tn10 : 47% pgm /zbf-507::Tn10 : 93% trp-49 = Brenner's trp-8(Am) lacZ125 = Jacob's lacY14(Am) 29723, 30109, 30020 ME8453 SG1039 F- ilv::Tn5 araD139 DE(argF-lac)U169 flbB5301 deoC1 rpsL150 relA1 zaj-403::Tn10 tonA21 thi ptsF25 proCYA221 30016 ME8454 SG20043 tonA21 rpsL150 flbB5301 deoC1 relA1 thi ptsF25 araD139 DE(argF-lac)U169 cps-3::Tn10 DE(lon) 860, 30016, 3287 ME8455 SG20252 F- relA1 rpsL150 flbB5301 deoC1 tonA21 thi ptsF25 zba-300::Tn10 DE(lon-100)? DE(argF-lac)U169 araD139 30016 ME8456 SG20262 F- DE(argF-lac)U169 relA1 rpsL150 flbB5301 deoC1 tonA21 thi ptsF25 DE(lon) his-2204::Tn10 araD139 30016 ME8457 YMC22 thi glnF208::Tn10 endA hsr DE(argF-lac)U169 Hut+ klebs hutCklebs supplement 1mg/ml glutamine 30030, 29721 ME8458 YMC26#3 endA glnD::Tn10 hutCklebs hsr Hut+klebs DE(lac)U169 thi 29816 ME8459 TH16#4 hutCklebs glnA::Tn5 DE(argF-lac)U169 Hut+klebs hsr thi endA T. Hunt supplement 1mg/ml Gln 30030, 4950, 35465 ME8460 LA5693 F- mtl-1 xyl-5 ara-14 galK2 his-4 proA2 argE3 thi-1 leuB6 thr-1 fts(BUG6) supE44 sup-37 zab::Tn10 proC::Tn5 DE(argF-lac)U169 tsx-33 str-31 29849 ME8461 BD18 Hfr F HfrH(valS <- uxuAB) DE(crp)96 rpsL DE(cya)851 thi zhd-732::Tn10 3187, 29849 ME8462 JB42 Hfr F HfrG6(metC <- argG) malTC-1 lamB102 zjb-729::Tn10 29849 ME8463 Pop1153 Hfr F HfrG6(metC <- argG) malE254 his 29849 ME8464 MM310 F- zja-742::Tn10 relA deoC1 flbB malTC-1 tonA21 thi ptsF25 araD139 DE(argF-lac)U169 rpsL150 30072 ME8465 JB100 Hfr F HfrG6(metC <- argG) ompR::Tn10 malTC-1 29849 ME8466 JB105 Hfr F HfrG6(metC <- argG) ompR::Tn10(TcS) malTC-1 zjb-729::Tn10 DE(mal)E444 29824 ME8467 RP1362 tar-362::Mu tsx-78 tonA31 xyl-5 mtl-1 ara-14 thi-1 rpsL136 DE(lac)U169 eda-50 metF(Am)159 his-4 dl(Ap,lac) 29833 ME8468 RP1365 metF(Am)159 eda-50 DE(lac)U169 rpsL136 thi-1 ara-14 mtl-1 xyl-5 tonA31 tsx-78 tap-365::Mudl(Ap,lac) his-4 29833 ME8469 RP5724 metF(Am)159 tsr-501::Tn5 leuB6 his-4 eda-50 rpsL136 thi-1 ara-14 lacY1 mtl-1 xyl-5 tonA31 tsx-78 29833 ME8470 BW6160,CGSC6756 Hfr F HfrB8(lac <- tsx) relA1 zdh-57::Tn10 (lambda)- lambda_R metB1 spoT1 CGSC(B.Bachmann & M.Berlin) ME8471 BW7620,CGSC6813 Hfr F HfrKL99(fadA <- pyrC) relA1 spoT1 thi-1 lac-42 (lambda)- zed-977::Tn10 CGSC(B.Bachmann & M.Berlin) ME8472 BW5660,CGSC6753 Hfr F HfrPK19(flaD <- supD) relA1? srl-300::Tn10 supE44 DE(gpt-lac)5 thi-1 (lambda)- spoT1? CGSC(B.Bachmann & M.Berlin) ME8473 BW6159,CGSC6755 Hfr F HfrKL14(tolC <- cca) ilv-691::Tn10 (lambda)- relA1 spoT1 thi-1 CGSC(B.Bachmann & M.Berlin) ME8474 BW6175,CGSC6763 Hfr F HfrPK3(malA <- xyl) lacY1 thr-1 leuB6 azi-15 tonA21 thi-1 argE86::Tn10 (lambda)- supE44 CGSC(B.Bachmann & M.Berlin) ME8475 BW6164,CGSC6759 Hfr F HfrRa-2(rha <- metB) thr-43::Tn10 supE42 (lambda)- malB28(lambda)R Sfa-4 CGSC(B.Bachmann & M.Berlin) ME8477 BW6165,CGSC6760 Hfr F HfrP801(leu -> pan) lambda_ind- argE86::Tn10 mtl-2 xyl-7 lacY1 or lac-40 ara-41 CGSC(B.Bachmann & M.Berlin) ME8478 BW6156,CGSC6754 Hfr F HfrP4X(argF -> lac) (lambda)- relA1 spoT1 metB1 zie::Tn10 CGSC(B.Bachmann & M.Berlin) ME8479 BW7261,CGSC6787 Hfr F HfrC(purE -> fep) T2R leu-63::Tn10 tonA22 DE(argF-lac)U169 ompF627 relA1 spoT1 CGSC(B.Bachmann & M.Berlin) ME8480 BW7623,CGSC6815 Hfr F HfrB7(ksgD -> trg) spoT1? relA1? (lambda)- purE79::Tn10 CGSC(B.Bachmann & M.Berlin) supplement 40_micro_g/ml Ade ME8481 BW7622,CGSC6814 Hfr F HfrKL96(his -> mgl) spoT1 (lambda)- relA1 trpB114::Tn10 thi-1 CGSC(B.Bachmann & M.Berlin) 856 ME8482 BW5659,CGSC6752 Hfr F HfrKL98(dsdA -> supN) mglP1 lacY1 xyl-7 (lambda)- zdh-57::Tn10 CGSC(B.Bachmann & M.Berlin) ME8483 BW6163,CGSC6758 Hfr F HfrKL16(lysA -> serA) relA1 (lambda)- zed-977::Tn10 spoT1 thi-1 CGSC(B.Bachmann & M.Berlin) ME8484 BW6169,CGSC6762 Hfr F HfrAB313(rbs -> ilvE) tsx-1? gal-6 (lambda)- argA81::Tn10 thi-1 leuB6 tonA2? lacY1 or lacZ4 supE44 CGSC(B.Bachmann & M.Berlin) ME8485 BW6166,CGSC6761 Hfr F HfrJ4(P10)(argE -> purA) malB16 supE44 (lambda)- lambda_R zhf-721::Tn10 thi-1 CGSC(B.Bachmann & M.Berlin) F is intefrated in malB. ME8486 BW2000 Hfr F HfrG6(metC <- argG) mgl::Tn10 his 29872 ME8487 BW2001 Hfr F HfrG6 galP::Tn10 his 29872 ME8488 21336 F- supE44 mtl-1 tsx-33 rpsL31 xyl-5 sup-37 uvrD260::Tn5 thr-1 leuB6 proA2 his-4 thi-1 argE3 lacY1 galK2 ara-14 21336 = GW3703 uvrD260::Tn5 = AB1157 uvrD260::Tn5 29865 ME8489 YYC162 Hfr F HfrC(purE -> lip) λ+ lambda_cI857 poxB1 pps-4 pfl-1 DE(aroP-aceEF) rpsL 30047, 30078 ME8490 YYC199 Hfr F HfrC(purE -> fep) pfl-1 rpsL DE(aroP-aceEF) zbj::Tn10 pps-4 YYC180 CURING/Temp. (lambda_cI857)- TR 30078, 30047 ME8491 YYC176 zje-1::Tn10 ampA73 psd-2 30047, 30078 ME8492 RDK1481 xyl-5 mtl-1 supE44 kdgK51 pheA18::Tn10 sbcB15 recC22 recB21 argE3 his-4 thi-1 proA2 leu-6 thr-1 rpsL31 galK2 lacY1 ara-14 29834 ME8493 RDK1540 supE44 recN1502::Tn5 argE3 his-4 leu-6 proA2 thr-1 thi-1 rpsL31 galK2 lacY1 ara-14 xyl-5 mtl-1 kdgK51 29834 ME8494 RDK1541 lacY1 xyl-5 mtl-1 supE44 thi-1 rpsL31 galK2 ara-14 kdgK51 recO1504::Tn5 argE3 his-4 leu-6 proA2 thr-1 29834 ME8495 DV8 F- zha-6::Tn10 metB1 panD2 panF1 grown on XEb1 met beta_-ala 29838 ME8496 DV14 F- panF1 metB1 panD2 zhc-12::Tn10 grown on XEb1 met beta_-ala 29838 ME8497 DV46 F- aroE353 panD2 panF1 zhc-9::Tn10 grown on XEb1 met beta_-ala trp phe tyr Shi 29838 ME8498 MW1104 Hfr F cdh-4::Tn10 30024 ME8499 MW1300 Hfr F zii-1::Tn10 30024 ME8551 PK191,CGSC4316 Hfr F HfrPK19(cheC <- his) (lambda)- sup-56(unmapped amber suppressor) DE5 thi-28 CGSC(B.Bachmann & M.Berlin) 29912, 29906, 29965, 29942 ME8553 KL99,CGSC4242 Hfr F HfrKL99(pyrD <- pyrC) (lambda)- thi-1 rel-1 lac-42 CGSC(B.Bachmann & M.Berlin) 29942, 29906, 29965, 29912 ME8554 W3213(Hfr13),CGSC4453 Hfr F HfrPO57(lac <- proC) metB1 rel-1 CGSC(B.Bachmann & M.Berlin) ME8555 R3,CGSC4895 Hfr F HfrPO111(leu -> tonA) thr-1 leu-6 thi-1 lacY1 gal-3 mtl-2 xyl-7 ara-13 malA1 tonA2 lambda_R supE44 CGSC(B.Bachmann & M.Berlin) ME8556 KL209,CGSC4315 Hfr F HfrJ4(P10)(argE -> purA) malB16 (lambda)- lambda_R supE44 CGSC(B.Bachmann & M.Berlin) 29942, 29965, 29912, 29906 ME8557 KL25,CGSC4244 Hfr F HfrPO46(pyrE <- ilvE) supE42 (lambda)- CGSC(B.Bachmann & M.Berlin) 29906, 29942, 29965, 29912 ME8558 KL228,CGSC4318 Hfr F HfrAB313(rbs -> ilvE) leu-6 gal-6 lacY1 or lacZ4 (lambda)- supE44 thi-1 CGSC(B.Bachmann & M.Berlin) 29942, 29912, 29906, 29965 ME8559 KL16,CGSC4245 Hfr F HfrPO45(lysA -> serA) (lambda)- thi-1 rel-1 CGSC(B.Bachmann & M.Berlin) 29942, 29912, 29906, 29965 ME8560 AT978,CGSC4544 Hfr F HfrKL16(lysA -> serA) thi-1 dapE9 (lambda)- rel-1 CGSC(B.Bachmann & M.Berlin) 29980 ME8561 KL983,CGSC4240 Hfr F HfrKL98(dsdA -> supN) lacY1 or lacZ4 (lambda)- xyl-7 mglP1 CGSC(B.Bachmann & M.Berlin) 29942, 29912, 29906, 29965 ME8562 KL96 (=AT2572),CGSC4243 Hfr F HfrPO44(his -> purF) thi-1 rel-1 (lambda)- CGSC(B.Bachmann & M.Berlin) 29942, 29965, 29906, 29912 ME8563 HFR44,CGSC4230 Hfr F HfrPO63(aroD -> his) ilv-282 argR40 argS41 argA42 CGSC(B.Bachmann & M.Berlin) 29942, 29968 ME8564 KL208,CGSC4314 Hfr F HfrB7(rac -> aroD) rel-1? (lambda)- CGSC(B.Bachmann & M.Berlin) This strain is suppressor-free. 29912, 29965, 29906, 29942 ME8565 9226-9 Hfr F HfrP4X argH thr leu thy his thi rif? ME8566 KY4124 Hfr F HfrH(pyrB <- thr) (lambda) rpsL140 relA1 met-82 mtr-24 29950, 29756 ME8567 AB1157-81-1 F- leu thr tsx gal proA his mtl xyl argE3 thi str nal ara 29869 ME8568 HB101 F- leu hsdS20(r-B,m-B) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 supE44 (lambda)- thi "This strain is a good host for large-scale growth and purification of plasmid.
sequence (FASTQ format)
https://ddbj.nig.ac.jp/search/entry/sra-run/DRR058061" 3251, 51302, 35355, 35598, 35597, 29962, 4960, 3303, 3253 ME8569 DH1 F- hsdR17(r-K,m-K) supE44 relA1? (lambda)- thi-1 gyrA96 endA1 recA1 KL16-99 MM294 CROSS 4967, 35422, 35552 ME8570 JM103 F+ F hsdR sbcB endA supE strA thi DE(lac-pro) A host for growth of the single-stganded phage M13 and its reconbinants. (E.coli K-12 E.coli B hybrid) 29962 ME8572 JM107 (RK-,MK+) F+ F DE(lac-proAB) hsdR17 supE44 relA1 traD36 endA1 gyrA96 thi 56353, 29828 ME8573 JM105 F+ F hsdR4 sbcB15 endA strA thi DE(lac-pro) JM105 contains partial supE activity. 29868 ME8575 N2058 F- proA2 ruv-59::Tn10 thi-1 his-4 argE3 thr-1 leuB6 ara-14 lacY1 galK2 xyl-5 mtl-1 supE44 tsx-33 rpsL31 29839, 30070 ME8576 CS81 galK2 xyl-5 mtl-1 supE44 tsx-33 proA2 his-4 thi-1 ruv-52 eda-51::Tn10 thr-1 rpsL31 argE3 leuB6 ara-14 lacY1 30070, 29839 ME8577 JC12334 F- tsx-33 rpsL31 tna-300::Tn10 recF143 thi-1 his-4 proA2 argE3 thr-1 leuB6 ara-14 lacY1 galK2 xyl-5 mtl-1 supE44 30039, 3201, 30091 ME8578 CS101 F- xyl-5 recA269::Tn10 ruv-51 thi-1 his-4 argE3 thrB1007 mtl-1 gyrA262 supE44? tsx-33? rpsL31 30039, 30091 ME8579 FB191 d(ApR lac) DE(pro-lac)XIII mtl-1 xyl-5 supE44? tsx-33? eda-51::Tn10 thi-1 argE3 uvrA278::Mu 30091, 30039 ME8580 N1671 Hfr F HfrC(purE -> lip) car-98::Tn10 relA1 tonA221 K. B.Low 30039, 30091 ME8581 N1672 Hfr F HfrH(valS <- uxuAB) thi-1 relA1 lacZ98::Tn10 30039, 30091 ME8582 KF1053 Hfr F HfrPO45(thyA -> serA) recE101::Tn10 ilv-318 thr-300 sbcA111::Tn5 SDB1041 29982, 30042 ME8583 KF1127 F- supE44 zde-264::Tn10 uidA1 argE3 lacY1 galK2 manA4 mtl-1 tsx-29 KF1102 GMS407 TcR 30042, 29982 ME8584 KF1225 F- mtl-1 zdd-263::Tn5 argE3 lacY1 galK2 manA4 tsx-29 supE44 uidA1 KF1166 GMS407 KmR 3283, 35355, 30042, 29982 ME8585 KF1271 F- lambda_R (lambda)- zdd-262::IS10-CamR-IS10 leuB6 tonA2 lacY1 or Z4 tsx-1 supE44 ksgB1 argG6 rpsL104 malA1 xyl-7 mtl-2 metB1 KF1260 FS173 29982, 30042 ME8586 KF1298 F- mtl-1 tsx-29 supE44 uidA1 zdc-261::Tn5 argE3 lacY1 galK2 manA4 KF1290 GMS407 30042, 29982 ME8587 KF1322 F- deo-27 his-317 [rfa]_phi_XS-EcoliC zdc-261::Tn5 zdb-260::Tn10 lacZ4 gal-44 supE44 endA1 KF1298 JC4583 29982, 30042 ME8588 KF1366 F- zdc-261::Tn5 zde-264::Tn10 zda-268::IS10-CamR-IS10 leuB6 tonA2 lacY1 or Z4 tsx-1 supE44 ksgB1 argG6 rpsL104 malA1 lambda_R xyl-7 mtl-2 metB1 (lambda)- KF1290 FS173 30042, 29982 ME8589 KF1344 F- endA1 deo-27 [rfa]_phi_XS-EcoliC supE44 gal-44 lacZ4 sbcA6 recC22 recB21 zda-268::IS10-CamR-IS10 KF1325 JC5176 30042, 29982 ME8590 PLK1110 zde-234::Tn10 rpsL supE trp pheA argA BD342 30043 ME8591 PLK1241 F- iclR7 zdd-230::Tn9 fnr-1 pyrF287 DE(trp)E5 gal-25 rpsL195 rac trpR72 PLK1220 PLK983 TcR 30043 ME8592 PLK1273 F- zdc-235::Tn10 rac iclR7 trpR72 rpsL195 gal-25 DE(trp)E5 pyrF287 fnr-1 PLK983 30043 ME8593 PLK1165 F- DE(rac) trg-2::Tn10 trpR trpA9605 his-29 ilv pro arg thyA deoB or deoC tsx RP3342 PLK1091 TcR 30043 ME8595 BD1467 GlyA::Tn5 Hfr F HfrPO45(thyA -> serA) relA1 glyA::Tn5 tyrA::Tn10 nadB ung-1 thi CBK081 BD1467 glyA::Tn5 KmR ME8596 BD1467 DapA or E::Tn5 Hfr F HfrPO45(thyA -> serA) thi relA1 dapA or E::Tn5 tyrA::Tn10 nadB ung-1 CBK110 BD1467 dapA or E::Tn5 KmR supplement 50_micro_g/ml DAP ME8597 BD1467 PurF::Tn5 Hfr F HfrPO45(thyA -> serA) tyrA::Tn10 purF::Tn5 ung-1 nadB thi relA1 CBK142 BD1467 purF::Tn5 KmR ME8598 BW5659 PurF::Tn5 Hfr F HfrKL98(dsd -> supN) xyl-7 zdh-57::Tn10 lacY1 purF::Tn5 (lambda)- mglP CBK142 BW5659 purF::Tn5 KmR ME8599 PK191 Zeg-722::Tn10 Hfr F HfrPK191(flaD <- serU) zeg-722::Tn10 thi DE(lac-proB) supD56 LA5651 PK191(ME=5484) zeg-722::Tn10 TcR ME8600 DG30 F- galK2 lacY1 hppT29 hsdS14 tyrB507 thi-1 mtl-1 rpsL31 tsx-33 supE44 recB21 recC22 sbcB15 (lambda)- argE3 ilvE12 hisG4 aspC13 proA2 xyl-5 tyrB507 and aspC13 requires 50_micro_g/ml Tyr and Phe even in L-broth 29736, 29720 ME8601 DG34 F- thi-1 proA2 aspC13 his-4 ilvE12 argE3 hsdS14 hppT29 lacY1 galK2 xyl-5 mtl-1 rpsL31 tsx-33 (lambda)- supE44 recB21 recC22 sbcB15 rpsL31 = strA31 aspC13 requires 50_micro_g/ml Tyr and Phe even in L-broth 29720, 29736 ME8602 DG44 F- supE44 hppT29 lacY1 galK2 xyl-5 mtl-1 rpsL31 tsx-33 (lambda)- recB21 recC22 sbcB15 hsdS14 tyrB507 thi-1 argE3 his-4 aspC13 proA2 rpsL31 = strA31 tyrB507 and aspC13 requires 50_micro_g/ml Tyr and Phe even in L-broth 29736, 29720 ME8603 RE103 F- cmlA1 strA101 trp-30 his-51 lac-28 proA23 strA101 = rpsL101 29949, 29995, 29940 ME8604 JP2144 F- trpA9605 tyrR366 his-85 ilv-632 tsx-84 (lambda)- The trp and his markers are suppressed by amber suppressers. 29917 ME8605 CT101 F- hpcR1 sad-1 This is a derivative of E.coli C. 30107, 30101 ME8606 RC63 F- thi-1 gal-55 ansA3 relA1 spoT1 hsdR4 (lambda)- 30077 ME8607 S_phi_1024 Hfr F HfrPO1(valS <- attP4) thi-1 nupC510::Tn10 relA1 metC69 spoT1 (lambda)- ME8608 S_phi_1023 Hfr F HfrPO1(valS <- attP4) thi-1 spoT1 nupG511::Tn10 relA1 (lambda)- ME8609 JM2267 F- cts rpsL150 relA1 DE(his-gnd)295 flbB5301 araD139 DE(argF-lac)169 (lambda)- deoC1 Ap gor-1::Mu araD139 was transduced in from E. coli B. 30035 ME8610 CO100 Hfr F HfrPO2A(purE -> fep) T2R tonA22 ompF627 relA1 pit-10 spoT1 dgoA1 29731 ME8611 K01 Hfr F HfrPO3(argF -> lac) kdgK1 metB1 relA1 spoT1 29884 ME8612 S1316 F- deoC1 (lambda)- araD139 DE(argF-lac)169 bioA24 zbh-428::Tn10 flbB5301 ptsF25 relA1 rpsL150 bisC9::Mu cts Co-td chlA/zbh-428::Tn10 : 90% The thr-leu region of this strain, including araD139, came from E. coli B. 30099 ME8613 GT3 Upp- ush 30040 ME8614 GM33[pLA7],CGSC5126 Other (lambda)- dam-3 IN(rrnO-rrnE)1 sup-85 CGSC(B.Bachmann & M.Berlin) selective plates should not be incubated for longer than 24hrs 30071 ME8615 AC4 pyrE ush purA 30040 ME8616 KL723 F+ F thi-1 thr-1 leuB6 proA2 his-4 recA13 argE3 ara-14 lacY1 galK2 xyl-7 mtl-1 rpsL31 tsx-33 (lambda)- supE44 This strain should be maintained on medium containing thi, arg, his and lacking leu, pro, thr. 29906 ME8618 KL719,CGSC4282 F tsx-67 supE44 (lambda)+ tonA23 rpsL109 azi-6 mtl-1 xyl-5 lacZ36 ara-14 thi-1 metE70 recA1 trpE38 purE42 proC32 leuB6 CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium containing B1, met, try, leu and lacking ade, pro. 29934 ME8619 KL718,CGSC4287 F+ F malA1 pyrD34 trp-45 his-68 tyrA2 recA1 thi-1 galK35 xyl-7 mtl-2 rpsL118 lambda_R (lambda)- CGSC(B.Bachmann & M.Berlin) This strain should be maintained on a medium containing thi, ura, try, his, tyr and with galactose as carbon source. rpsL118 = strA118 F'152 is also known as the F2-gal of Jacob 29906, 29749 ME8620 KL731,CGSC4254 F+ F supE44 lambda_R (lambda)- tsx-1 tonA2 rpsL104 malA1 mtl-2 xyl-7 gal-6 lacY1 metB1 argG6 thyA23 recA1 hisG1 leuB6 CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium containing arg, his, leu, met and lacking thy. rpsL104 = strA104 29906 ME8621 KL708,CGSC4248 F+ F lambda_R (lambda)- leuB6 hisG1 recA1 argG6 metB1 lacY1 gal-6 xyl-7 mtl-2 malA1 rpsL104 tonA2 tsx-1 supE44 CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium containing his, leu, met and lacking arg. This strain grows slowly. rpsL104 = strA104 29906 ME8622 MAF1/JC1553,CGSC4289 F rpsL104 leuB6 hisG1 recA1 argG6 metB1 lacY1 gal-6 xyl-7 mtl-2 malA1 tonA2 tsx-1 supE44 lambda_R (lambda)- CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium leu, his, met and lacking arg. It grows slowly on such a medium. It gives a Mtl phenotype on MacConkey-Mtl medium. rpsL104 = strA104 29906 ME8623 DFF1/JC1553,CGSC4326 F+ F supE44 malA1 mtl-2 xyl-7 gal-6 lacY1 metB1 argG6 recA1 hisG1 leuB6 lambda_R (lambda)- rpsL104 tonA2 tsx-1 CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium containing leu, met, arg and lacking his. F150 is reported to have deleted uvrC. rpsL104 = str104 29984 ME8624 KL704,CGSC4280 F+ F hisG1 tsx-1 recA1 argG6 metB1 lacY1 mtl-2 gal-6 xyl-7 tonA2 rpsL104 malA1 supE44 lambda_R (lambda)- leuB6 CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium containing leu, arg, met, and lacking his. rpsL104 = strA104 29906 ME8625 KL709,CGSC4279 F+ F xy1-7 pyrD34 trp-45 his-68 tyrA2 recA1 thi-l galK35 mtl-2 malA1 rpsL118 lambda_R (lambda)- CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium containing thi, his, try, ura and lacking tyr. Watch out for faster-growing clones bearing further partial deletions of F142. rpsL118 = strA118 29906 ME8626 KL711,CGSC4291 F+ F his-68 tyrA2 recA1 thyA33 thi-1 galK35 xyl-7 mtl-2 malA1 rpsL118 lambda_R (lambda)- pyrD34 trp-45 CGSC(B.Bachmann & M.Berlin) This strain should be maintaing on medium containing thi, his, ura, try, thy. F143 probably carries the relA1 mutation. rpsL118 = strA118 29906 ME8627 KL701,CGSC4256 F+ F lambda_R trp-45 his-68 recA1 thi-1 galK35 xyl-7 mtl-2 malA1 rpsL118 pyrD34 (lambda)- CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium containing thi, ura, his and lacking try. rpsL118 = strA118 29906 ME8628 KL703,CGSC4253 F+ F malA1 mtl-2 xyl-7 galK35 thi-1 recA1 his-68 trp-45 pyrD34 lambda_R (lambda)- rpsL118 CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium with galactose as carbon source, containing thi, his, and lacking ura, try. The strain shows a Gal phenotype on MacConkey-Gal medium. rpsL118 = strA118 29906 ME8629 F500/GMS724,CGSC5505 F+ F supE44? lacY1 galK2 man-4 rpsL700 tsx-29? recA1 aroD6 metB1 CGSC(B.Bachmann & M.Berlin) This strain should be maintained on minimal medium with mannose as carbon source containing met and lacking aromatic amino acids. rpsL700 = strA700 29767 ME8630 F506/JE5519,CGSC5760 F+ F rpsL aroD argE lac gal man nalA recA1 CGSC(B.Bachmann & M.Berlin) 29737, 29746 ME8631 KL728,CGSC4258 F+ F lambda_R leuB6 hisG1 recA1 argG6 metB1 lacY1 gal-6 xyl-7 mtl-2 malA1 rpsL104 tonA2 tsx-1 supE44 (lambda)- CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium containing arg, his, leu, and lacking met. rpsL104 = strA104 29906 ME8632 KL706,CGSC4265 F+ F (lambda)- leuB6 hisG1 recA1 argG6 metB1 lacY1 gal-6 xyl-7 mtl-2 malA1 rpsL104 tonA2 tsx-1 lambda_R CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium containing arg, his, leu and lacking met. rpsL104 = strA104 29906 ME8633 KL729,CGSC4260 F+ F (lambda)- supE44 lambda_R tsx-1 tonA2 rpsL104 malA1 mtl-2 xyl-7 gal-6 lacY1 metB1 argG6 recA1 hisG1 leuB6 CGSC(B.Bachmann & M.Berlin) This strain should be maintained on medium containing arg, his, leu and lacking met. It is a slow-grower. Watch out for fast-growing clones that have lost all or part of F112. F'112 may carry mal and 28lambda_R 29906 ME8635 KL720,CGSC4247 F+ F lambda_R supE44 malA1 xyl-7 mtl-2 rpsL104 tonA2 tsx-1 (lambda)- leuB6 hisG1 recA1 argG6 metB1 lacY1 gal-6 CGSC(B.Bachmann & M.Berlin) This strain must be maintained on medium containing his, leu, met and lacking arg. ME8637 AB3254 F- aroH367 thi-1 argE3 proA2 galK2 lacY1 tsx-29 30008 ME8639 AT722 F- thi-1 pyrE41 argG6 rbs-1 malA1 str supE44 his pro thr leu B. Bachmann ME8640 GM33 dam-3 W3110 ME8643 pUC-4K/JE7853-8 RecA Hfr F HfrC(purE -> lip) srl::Tn10 recA56 his 30051 ME8644 BW9109 thi-1 xyl-5 hisG4(ochre) argE3(ochre) lacY1 galK2 ara-14 mtl-1 supE44(Am SuII) rpsL31 tsx-33 DE(xth-pncA)90 Rac- leuB6(Am SuIII) thr-1 DE(gpt-proA)62 BW9101 BW171 Pps+ Bernard Weiss DE(xth-pncA)90 + markers of AB1157 29768 ME8648 MM383::Tn5 F- polA12 thy rha str lac zig::Tn5 R. Fukuda -> H. Hara 29888 ME8649 JM83 φ80- ara DE(lac-proAB) rpsL (phi_80)+ (lacZ)DE(M15) 29828, 56351 ME8651 YN27 F+ F tsx argG metB his leu recA supE44 mtl xyl mal gal lacY str tonA Nagata ME8652 YN34 F+ F lacY metB his leu recA supE44 mtl xyl mal gal argG str tonA tsx Nagata ME8653 YN33 F+ F tsx tonA str lacY gal mal xyl mtl supE44 recA leu his metB argG Nagata ME8654 YN41 F+ F mal argG metB his leu recA supE44 mtl xyl gal lacY str tonA tsx Nagata ME8655 YN28 F+ F his proA recA mtl xyl ara galK lacY str tsx supE44 thi thr leu argE Nagata ME8656 IQ105 F+ F mtl thi pyrD his trp recA xyl malA galK str Nagata ME8657 IQ108 F+ F strA galK malA xyl mtl recA tyrA trp his pyrD thi Nagata ME8658 YN38 F+ F tsx supE trpE leu thi metE purE proC recA mtl xyl ara lacZ str azi tonA Nagata IQ109 ME8660 YN50 F+ F xyl argG metB his thyA leu recA mtl malA gal lacY str tonA tsx supE44 Nagata ME8661 IQ112 F+ F xyl tonA thyA thi pyrB relA ara lacY gal malA rpsL (lambda)- thr leu pro hisG Nagata ME8662 IQ114 F+ F met? Nagata ME8663 IQ115 F+ F arg? Nagata ME8671 C600 Zia-138::Tn10 F- lacY1 thi-1 tonA21 thr-1 zia-138::Tn10 leu-6 supE44 ME8280 (=CH1525) C600(#1) TcR ME8679 JE8501 F- purE dapD2 argG ile thi ara his galK malA xyl mtl str trp leu ME8680 E486 F- thi leu thr str dnaE486 lac thy 4914, 861 ME8681 JE7094 Mal+ F- metD proA metB argG dap lys str DE(lac) tsx 30182 ME8684 GIA37 Mal+ F- argF pyrF thyA his leu argI supE phx azi tsx mal lacY str codA 29922 ME8686 JE7858 F+ F purE tsx::Tn5 proC zai::Tn10 araA ME8687 607 plsA F- lys adk(=plsA) str thi trp metE proC leu xyl ara tonA tsx Ts mutant 30050 ME8692 PSM3 F- glnP glnPo metC thi 30029 ME8695 KL285 F- serC13 serS14 trp-45 his-68 thi-1? rpsL118 malA1 xyl-7 mtl-2 Ts(-NaCl) 29914 ME8697 KT1042 F- tsx tonA trp leu lacY gal his argG ile metB xyl mtl thi str ara dirE42 Ts 30092 ME8701 PA340 F- rpsL9 lambda_R (lambda)- supE44 thr-1 gltB31 tonA2 gdh-1 mtl-2 xyl-7 malA1 gal-6 lacY1 ara-14 argH1 hisG1 thi-1 leuB6 29721 ME8707 JE5513 Hfr F HfrC(purE -> lip) lpp man pps 29724 ME8708 FS173 F- λ+ metB1 ksgB1 leuB6 tonA2 lacY1 or 84 tsx-1 supE44 argG6 rpsL104 malA1 xyl-7 mtl-2 (lambda)+ 29982, 30042 ME8709 JP1112 Hfr F HfrH? galE thi pheS353 str Ts 29990 ME8715 11-2 F- his-4 strA136 nalA pgsA444 pgsB thr-1 leu-6 Ts 29757 ME8723 E101 F- str-9 supE44 thy leu-6 nrdA tonA21 lacY1 thi-1 thr-1 Ts ME8728 E729 F- ara-13 mtl-2 tonA2 supE44 purI66 nadB4 thi-1 argH1 str-9 lacY1 gal-6 malA1 xyl-7 purI66 = purL E729 = PA3306 29980 ME8732 A74 Mal+ F- metB1 argG6 mtl-2 str-50 xyl-7 alaS3 his-1 leu-6 Ts 29931 ME8734 AT713 Mal+ Cys+ F- (lambda)- supE44? argA21 lysA22 thi-1 str-104 mtl-2 xyl-7 ME8736 MA163 Mal+ F- supE44? ara-13 gal-6 tonA2 (lambda)- mtl-2 lacY1 strA133 hisG1 thi-1 leuB6 thr-1 speA1 xyl-7 MA163 = JE6663 29902 ME8739 PC3 F- leu thy str dnaG3 ME8742 W945 T1-2_lambda_- F- galK2 mdh-2 thr-1 leuB6 trp-59 thi-1 ara-14 lacY1 xyl-5 mtl-1 (lambda)- supE44? rpsL265 29777, 29971 ME8743 D23 F- sloB1 his-51 trp-30 proA23 lac-28 strA173 ampA1 tsx-81 envB1 29782, 29959 ME8744 L8 F- galK2 fabE22 thi-1 gltA5 str-20 ara-14 lacY1 xyl-5 mtl-1 tfr-5 tsx-57 Ts 29719 ME8750 PA3092 F- tonA mel malA xyl mtl lacY str trp his thy argH thi leu thr ME8752 MFT1181 Mal+ F- tonA ftsE1181 thr leu thi argH thy his trp str lacY mtl xyl mel Ts ME8758 LC173 Mal+ F- thr thi dnaA46 thy ilv lac leu ME8758 = CRT46 Mal+ Ts ME8765 9223 F- str his thi rpoC92 lac Ts 29743 ME8766 PA505 (MB)DE(101c) Mal+ F- Other P1 metA thy arg (P1)+ str ME8767 PA505 (MB)DE(101c) F- Other P1 thy metA str (P1)+ arg DE(malB) ME8768 JGC155 F- λ+ ssb-1 gal (lambda)+ str Ts 29732 ME8769 PA3092 Mal+ F- thr lacY mtl his xyl mel tonA argH trp thy str thi leu ME8770 CS8 AspTL Hfr F HfrC(purE -> lip) T2R metB1 rel-1 tonA22 gltC8 aspA22 29775, 29964 ME8775 PC2 F- dnaC2 leu thi lac str Ts 3233 ME8778 KL235 Hfr F HfrC(purE -> lip) supD32 relA1 tonA22 pit-10 T2R spoT1 serS15 phoA(Am) Ts 29988 ME8779 LC102 Thy+RecA1 str leu recA1 tsx purE trp(E?) thi his metB ile argG ara lac xyl mtl gal proC JE5595 (LC248 KL16-99) LC102 Thy- CROSS recA1 Thy+ StrR Y. Nishimura PurE- : other markers are not tested. ME8780 CH1692 DE(lac-514) tolC::Tn10 topA57(Am) argA supD74(ts) rpsL 29804 ME8971 CAG5051 Hfr F HfrH(valS <- uxuAB) relA1 supQ80 nadA57::Tn10 thi-1 spoT ME8893(=CAG18341) HfrH nadA57::Tn10 TcR M. Singer 29803 ME8972 CAG12206 Hfr F HfrH(valS <- uxuAB) spoT supQ80 thi-1 nadA3052::Tn10kan relA1 ME8798(=CAG12147) HfrH nadA3052::Tn10kan KmR M. Singer 29803 ME8973 CAG5052 Hfr F HfrKL227(89' -> 6') btuB3191::Tn10 relA1 metB1 CAG3032 KL227 btuB3191::Tn10 TcR M. Singer 29803 ME8974 CAG12204 Hfr F HfrKL227(89' -> 6') btuB3192::Tn10kan relA1 metB1 CAG8408 KL227 btuB3192::Tn10kan KmR M. Singer 29803 ME8975 CAG5053 Hfr F HfrKL208(12' -> 35') relA1 zbc-3105::Tn10 CAG12123 KL208 zbc-3105::Tn10 TcR M. Singer 29803 ME8976 CAG12203 Hfr F HfrKL208(12' -> 35') relA1 zbc-3200::Tn10kan CAG12046 KL208 zbc-3200::Tn10kan KmR M. Singer 29803 ME8978 CAG12202 Hfr F HfrKL96(his -> mgl) thi-1 trpB3193::Tn10kan relA1 CAG18579(=ME8904) KL96 trpB3193::Tn10kan KmR M. Singer 29803 ME8979 CAG5055 Hfr F HfrKL16(thyA -> serA) thi-1 zed-3069::Tn10 relA1 ME8916(=CAG18563) KL16 zed-3069::Tn10 TcR M. Singer 29803 ME8980 CAG12200 Hfr F HfrKL16(thyA -> serA) zed-3128::Tn10kan relA1 thi-1 ME8823(=CAG18451) KL16 zed-3128::Tn10kan KmR M. Singer 29803 ME8981 CAG8160 Hfr F HfrKL14(tolC <- cca) relA1 thiA39::Tn10 leu CAG18616 KL14 thiA39::Tn10 TcR M. Singer 29803 ME8982 CAG12201#1 Hfr F HfrKL14(tolC <- cca) thiA3178::Tn10kan leu relA1 ME8868(=CAG18500) KL14 thiA3178::Tn10kan KmR M. Singer 29803 ME8983 CAG8209 Hfr F HfrKL228(rbs -> ilv) supE44 thi-1 gal lacY or Z zgh-3075::Tn10 leu ME8937(=CAG18574) KL228 zgh-3075::Tn10 TcR M. Singer 29803 ME8984 CAG12205 Hfr F HfrKL228(rbs -> ilv) leu lacY or Z gal thi-1 supE44 zgh-3159::Tn10kan CAG18164 KL228 zgh-3159::Tn10kan KmR M. Singer 29803 ME8985 K91 Hfr F HfrC ompF627 phoA6 tonA22 garB10 relA1 pit-10 spoT1 T2R PO2A K38=Garen's S26 lambda_- Horiuchi ME8986 K1141 Hfr F HfrC spoT1 PO2A garB10 ompF627 relA1 pit-10 T2R himA[DE(82::Tn10)] phoA6 tonA22 ME8985(=K91) himA[DE(82::Tn10)] TcR Horiuchi ME8987 JA221 F lpp trpE5 leuB6 lacY recA1 29758 ME8988 MM52 F- thi secA DE(lac)U169 araD136 relA rpsL ME8286(=MC4100) Ts-growth 30108 ME8989 W3110ColE1 Other IN(rrnD-rrnE)1 ME8990 K1151 Hfr F HfrC garB10 ompF627 relA1 pit-10 spoT1 hip[DE(3::camR)] phoA6 PO2A tonA22 T2R ME8985(=K91) hip[DE(3::camR)] CMR Horiuchi ME8991 K1174 Hfr F HfrC tonA22 garB10 ompF627 relA1 pit-10 spoT1 T2R PO2A phoA6 hip[DE(3::camR)] himA[DE(82::Tn10)] ME8985(=K91) himA[DE(82::Tn10)],hip[DE(3::camR)] TcR CMR Horiuchi ME8992 JM109#6 F+ F relA1 supE44 hsdR17 thi-1 gyrA96 endA1 pro DE(lac) recAl ME8993 QC774 F- DE(lac)4169 rpsL DE(sodA-lacZ)49 DE(sodB-kan)1-DE(2) CmR KmR S.Yonei 864, 35249, 29848, 29840, 865 ME8994 C600 Su- F- thr-1 tonA21 lacY1 thi-1 leu-6 ME6040 ME8020(=C600) TD./Others (T4GT7) Su- (T4AmC)R ME8994=(T4AmC)R (T4W)S lacY1,tonA21=not tested ME8995 CSH26 PolA12(Tn5)#6 F- thi DE(lac-pro) ara polA12(Tn5) ME8996 AD202 F- tonA21 thi ptsF25 araD139 relA1 rpsL150 flbB5301 deoC1 ompT::Km DE(argF-lac)U169 AD179 ME8286(=MC4100) K. Ito Km 10_micro_g/ml AD202=MC4100 OmpT::Km 74361, 29800 ME8998 B178 galE Y. Yamamoto B178=W3110 galE 4969 ME9000 W3110 Dam-13::Tn9 IN(rrnD-rrnE)1 dam-13::Tn9 Y. Nishimura 30053 ME9001 CT28-3b thr leu his pro arg str dnaC28 thy thi Schubach et al. 29910 ME9002 P678 F- ara-13 tonA2 lacY1 supE44 gal-6(galb) rfbD1 galP63 malT1(lambda_R) xyl-7 mtlA2 thi-1 thr-1 leuB6 Y. Nishimura ME9003 K866 F+ F relA1 his-871 rep-71 gal-3 rpsL181 Y. Nishimura 30048 ME9011 TK2 ME9012 K12 F+ λ+ Y. Nishimura 74357, 3954, 84966 ME9013 Ecoli CR sup S. Yasuda Su+ ME9014 Ecoli Cla S. Yasuda ME9015 E.coli B/r WP2 trp JAPAN BIO ASSAY RESEARCH CENTER UVR 30194 ME9016 E.coli WP2 hcr- trp uvrA JAPAN BIO ASSAY RESEARCH CENTER UVS 30194 ME9017 CRT46 F- dnaA(T46) mal lacY ilv thi thy leu thr Y. Hirota 29976 ME9018 DPB271(=BIK804) F- recD1903::mini-tet (lambda)- 859, 29842, 4926, 30025 ME9019 FS1576 supE44 tonA21 lacY1 recD1009 leuB6 thr-1 thi-1 29841 ME9020 CJ236 Other relA1 dut1 ung1 thi-1 ME9021 BMH71-18 mutS F thi DE(lac-proAB) mutS215::Tn10 supE ME9022 MV1184 F ((phi_80lacZ)DE(M15)) DE(srl-recA)306::Tn10 DE(lac-proAB) ara strA thi ME9023 pCL1920/cL83 Other φ80- ara recA56 (phi_80)+ (lacZ)DE(M15) rpsL DE(lac-proAB) JM83 recA M. INOUYE Spectino mycin-resistance Strepto mycin-sensitive 29802 ME9024 pCL1921/cL83 Other φ80- ara (phi_80)+ rpsL (lacZ)DE(M15) recA56 DE(lac-proAB) JM83 TF. recA M. INOUYE Spectino mycin-resistance Strepto mycin-sensitive 29802 ME9025 GC3403 F- gal sfiA::Mud(Ap,lac)c62 trp::MuC+ DE(lac) thr leu pyrD thi malB(lambda_S) rpsL(strR) sfiC H. Ogura ME9026 JM109(DE3) rec1 DE3(T7 RNA polymerase) relA1 supE44 hsdR17 thi-1 gyrA96 endA1 pro DE(lac) JM109 S. Yasuda ME9027 KNK453(gyrAts) F- uvrA thyA polA nalA(Ts)=gyrAts phx Y. Nishimura 29733 ME9028 W3110 tna::Tn10 parA110 F- parA110(=gyrBts) tna::Tn10 Y. Nishimura 3305, 29805 JE5500 A5 F- lpm his thi argE lacY galK mtl xyl tsx JE5528 JE5588(=AB1360) CROSS lpm AroD+ MalB+ 35657 JE5505 1360aroD+ F- pps lpo5508 tsx-29 xyl-5 lacY1 galK2 thi-1 argE3 proA2 mtl-1 his-4 JE5510 AB1360(=JE5588) lpo AroD+ Y. Nishimura 29755, 29724 JE5506 1360aroD+lpo+4 F- pps mtl-1 tsx-29 thi-1 argE3 proA2 his-4 galK2 lacY1 xyl-5 JE5510 AB1360(=JE5588) AroD+ Y. Nishimura 29724, 29755 JE5507 S343spc F- argE3 galK2 lacY1 str-700 or -704 spc-12 man-1 aroD362 E5014(=JE5592) S343(=JE5593) spc SpcR Y. Nishimura 29724, 29755 JE5508 lpo-1 F- galK2 lacY1 str-700 or -704 spc-12 lpo5508 argE3 JE5517 JE5515 AroD+ Y. Nishimura 29724, 29755 JE5509 17-8 F- xyl-7 mtl-2 malA1 thi-1 argH1 his-1 lysA dapA pps man-1 str-9 lacY1 JE5502 JE5516 AroD+ Y. Nishimura 29724, 29755 JE5510 r11-16 F- thi-1 lpo5508 man-1 pps dapA lysA his-1 argH1 str-9 lacY1 xyl-7 mtl-2 malA1 JE5517 JE5801 lpo AroD+ Y. Nishimura Supplement 50_micro_g/ml DAP 29724, 29755 JE5511 HfrClpm2(=A'2) Hfr F HfrC(purE -> leuS) lpm pps man-1 JE5500 JE5504 lpm AroD+ Y. Nishimura 29755, 29724 JE5512 a19 Hfr F HfrC(purE -> leuS) pps man-1 JE5514 JE5504 AroD+ Y. Nishimura 29724, 29755 JE5513 a55 Hfr F HfrC(purE -> leuS) man lpo pps JE5514 JE5504 lpo AroD+ Y. Nishimura 3222, 29724, 29755 JE5514 1360 aroD+lpo-2 F- lpo his proA argE thi gal lac xyl mtl tsx JE5510 AB1360(=JE5588) lpo AroD+ JE5515 S343 spc gurA F- aroD arg EH gal xyl lac mtl str spc uidA1 GM291(JE5591) JE5507 uidA1 Man+ JE5518 3092DL F- lys dap trp mtl xyl lacY str malA his argH thi JE5707 PA3092 CROSS dap lys Thy+ StrR JE5520 1360 aroD+lpo+10 F- tsx mtl xyl lac gal thi argE proA his JE5510 AB1360(JE5588) AroD+ JE5523 r11-16 pps+ F- thi lpo man dap lys his argH str lac xyl mtl malA W3110 JE5510 Pps+ Supplement 50_micro_g/ml DAP JE5524 R11-16 Pps+Lpo+ F- man malA mtl xyl lac str thi argH his lys dap W3110 JE5510 lpo+ Pps+ Supplement 50_micro_g/ml DAP JE5525 F- str recA1 trp gal man pps lpm ME6236 = re-isolated single colony from JE5525 JE5528 G6 Lpm#7 Hfr F HfrG6(metC <- argG) malB lpm trp JE5596(=G6) MFT1033 CROSS lpm Xyl+ FtsD+ His+ ArgH+ Thi+ Thr+ leu+ JE5530 Pam1 F+ F Other Plkc lpm str gal JE5500 JE5501 lpm Pps+ 4976 JE5531 Ma1 F+ F lpm pps str gal JE5500 JE5501 lpm Man+ JE5535 Pa2 F+ F man str gal lpm JE5500 JE5501 lpm Pps+ JE5546 P1 F+ F aroD str gal man JE5500 JE5501 Pps+ JE5557 Am2 F+ F lpm pps JE5500 JE5501 lpm AroD+ JE5575 K63m3 F+ F his str gal fadD JE5500 K63(=JE5590) Man+ JE5589 K6-1,K6-A F+ F pps str proA2 aroD argA thi H. L. Kornberg aroD is from AB1359 Gur- (Novel & Novel MGG120:319 1973) 29933, 29885 JE5590 K63 F+ F fadD88 his-1 str gal-3 man 29952 JE5593 S343 F- mtl str argEH aroD man gal lac xyl G. Novel & M. Novel man is from K63(=JE5590) aroD is from AB3302 29909 JE5594 LC250, KL163 Hfr F HfrKL16(serA <- lysA) thy recA thi nalA L. Caro, B. Low NalR 10_micro_g/ml (weak resistance because of recA-) JE5595 LC248, KL16-99 Hfr F HfrKL16(serA <- lysA) thi recA1 L. Caro, B. Low JE5599 P4X8 Hfr F HfrP4X8(lac <- proA) metB1 F. Jacob 30172 JE5600 353 Hfr-29 Hfr F HfrP4X8(argF -> lac) tonA ponB353 ts thr leu thy his xyl str JE5599(=P4X8) JE10353 CROSS ponB353 Hfr Lac+ StrR P1.kc poor growth JE5601 X708 F- str arg leu proB lacZ R. CurtissIII JE5605 27aroB1 F- str aroB351 xyl dacA1191 dacB12 AB2826SR(JE5352) JE5691 aroB351 MalA+ JE5612 1a1b#47 F- str tsx DE(lac-72) proA tonA2 metB ponA1104ts ponB353 dacA1191 dacB12 JE5603 JE5606 CROSS ponB353 Xyl+ StrR lysed at 42C should be grown in 1% NaCl L broth JE5613 1a1b#47 Thy1 F- ponA1104ts ponB353 dacA1191 dacB12 thy tsx DE(lac-72) proA tonA2 metB str JE5612 SPONTANEOUS thy TrimethoprimR lysed at 42C should be grown in 1% NaCl L broth JE5615 lalb#47 DL2 F- dap ponB353 dacA1191 dacB12 lys ponAts1104 str metB tonA2 proA DE(lac)72 tsx JE5707 JE5613 CROSS dap lys Thy+StrR lysed at 42C. should be grown in 1% NaCl L broth. Supplement 50_micro_g/ml DAP JE5618 1a1b#47 DL23 F- tsx dacA1191 ponB353 ponAts1104 dacB12 lys dap str thi? tonA2 proA DE(lac)72 JE5707 JE5614 CROSS dap lys Thy+StrR lysed at 42C. should be grown in 1% NaCl L broth. Supplement 50_micro_g/ml DAP JE5619 1a1b#47 DL26 F- proA tsx DE(lac)72 tonA2 metB str dap lys? ponAts1104 ponB353 dacA1191 dacB12 JE5707 JE5614 CROSS dap lys? Thy+StrR lysed at 42C. should be grown in 1% NaCl L broth. Supplement 50_micro_g/ml DAP JE5623 #6-1 F- dacB12 metA or B thi ile mtl his gal trp proC lacY str xyl tsx ftsI730 dacA1191 W3110 JE5631 Leu+ JE5624 #6-2 F- metA or B dacA1191 str mtl xyl ile lacY proC tsx gal trp his thi dacB12 W3110 JE5631 ftsI730+ Leu+ JE5625 353 dac#36 F- dacA1191 dacB12 ts-353 tonA leu mtl xyl str gal tsx ponB353 JE5600 JE5630 CROSS ponB353 Ara+ Thy+ P1S. (thr thi metA or B ile his trp proC lacY) not tested JE5627 980 dac ml Hfr F HfrP4X8(argF -> lac) his str tonA dacA1191 dacB64 thy JE5608 JE5639 MalA+ TS on LA-NaCl JE5630 Pu6 AG1 F- trp proC ara mtl xyl lacY str tsx gal dacB12 dacA1191 leu thi metA or B ile his JE5662 JE5663 dacB12 ArgG+ TS on LA-NaCl JE5634 1a1b#32 LV1 F- tonA2 leuB ilvH611 tsx DE(lac)72 proA str ponAts1104 ponB353 dacA1191 dacB12 MI183e(=JE5774) JE5611 leuB ilvH ValR lysed at 42C. should be grown in 1% NaCl L broth JE5638 353 Hfr CmD2 Hfr F HfrC(purE -> lip) malA DE(lac)72 metB proA JE5602 ME6085(=CD4) MetD+ JE5641 L+ 1- F- DE(lac)72 dacB12 dacA1191 proA str tonA2 ponAts1104 ponB353 tsx JE5623 JE5634 Leu+ TS in 1% NaCl L broth JE5647 1a+1 F- tonA dap lys tsx DE(lac)72 metB spc proA? dacB12 dacA1191 ponAts1104 JE5711 JE5615 CROSS SpcR Leu+ Thi+ argG+ His+ Trp+ PurE+ Supplement 50_micro_g/ml DAP JE5649 1b+2 F- DE(lac)72 tsx lys dap str metB ara leu dacB12 dacA1191 ponAts1104 JE5711 JE5615 CROSS ponB353+ ProA+ Thi+ Ile+? StrR? ArgG+ His+ Trp+ PurE+ Supplement 50_micro_g/ml DAP JE5653 1a+1b+#1 F- mtl dacA1191 dacB12 leu metB ile? xyl spc dap lys tsx DE(lac)72 JE5711 JE5615 CROSS ponA+ ponB+ ProA+ SpcR ArgG+ His+ Trp+ PurE+ PBC-3-? Supplement 50_micro_g/ml DAP JE5654 4+#55 F- DE(lac)72 ponAts1104 dacA1191 proA? tonA metB str spc argG dap lys tsx JE5711 JE5615 CROSS dacB12+ SpcR Leu+ Thi+ Ile+ StrR His+ Trp+ PurE+ Supplement 50_micro_g/ml DAP JE5659 3ts IlvH#13 F- tonA tsx lysA ftsIts (MFT33) ilvH611 thi-1 metE peoC purE trp xyl lacZ str JE5651 MI183e(=JE5774) Leu+ JE5661 MS20 F- mtl dacB12 argG metC nalA his gal lac JE5662 JE5712 CROSS dacB12 MalA+ NalAR JE5664 maleB Hfr F HfrP4X8(argF -> lac) his dacA1191 JE5599(=P4X) JE5710 CROSS MalA+ MetB+ JE5665 C11 F- lambda_Rlambda_- ponA980 lysA argA his mtl supE mal-18? malA gal-6? JE5607 AT718(=JE5447) ponA980 CysG+ ponA is cotransduced with str JE5670 #2 F- DE(lac) str dap lys tonA proA? metB tsx dacB12 dacA1191 ponAts1104 JE5667 JE5649 CROSS Leu+ StrR Supplement 50_micro_g/ml DAP JE5671 #17 F- lys dap str metB tonA proA? DE(lac) tsx dacB12 dacA1191 ponAts1104 JE5667 JE5649 CROSS Leu+ StrR Supplement 50_micro_g/ml DAP JE5679 1238P F- rodA1238 lacY str P4x8(JE5599) JE11238 CROSS PlS Thr+ Leu+ Thy+ StrR Met+ (thi argH his trp mtl xyl malA)not tested JE5683 1a#1 F- DE(lac)72 ponA980 dacA1191 dacB12 leu? mtl xyl malA spc dap lys JE5607 JE5652 CROSS ponA980 Thi+ Met+ Ile+ SpcR Supplement 50_micro_g/ml DAP JE5684 1a#4 F- dacA1191 dacB12 leu? mtl xyl malA spc dap DE(lac)72 lys JE5607 JE5652 CROSS Thi+ Met+ Ile+ SpcR Supplement 50_micro_g/ml DAP JE5685 1b#23 F- proA? tonA ilvH metB leu malA spc dap lys proB353 dacA1191 dacB12 tsx DE(lac)72 JE5666 JE5652 CROSS ponB353 Xyl+ SpcR Supplement 50_micro_g/ml DAP JE5688 OV1 F- dacB12 ponAts1104 rodA(SP6) dap lys metB str sup-126? trpE(Am)? JE5713 JE5649 CROSS rodA(SP6) Lac+ StrR CSH(J.H.Miller) JE5694 B22 F- str argG JE5662 JE5775(=UTH6674) AspB+ JE5695 B23 F- str argG dacB12 JE5662 JE5775(=UTH6674) dacB12 AspB+ JE5700 JE5634/pLC19-19 F+ F Other tsx DE(lac) proA tonA str ponB353 dacA1191 dacB12 leu ilvH ponA1104ts JA200/pLC19-19 JE5634 CROSS pLC19-19 CefhaloridineR(1_micro_g/ml) StrR JE5702 85td6 F- DE(lac)? ponB85-21 ponA1104ts tsx? tonA metB str proA? dacB12 dacA1191 JE5637 JE5658 ponB85-21 MetD+ JE5707 CU4431Lys Hfr F HfrH(pyrB <- pil) dap lys thi Y73(JE5776) CU4431(JE5777) lys Thy+ tight Lys- JE5711 Hfr F HfrP4X8(argF -> lac) ile leu metA or B thi argG his trp purE ara mtl xyl gal spc JE5713 SP6male Hfr F HfrAB313(rbs -> ilv) thi? rodA(SP6) JE5779(=KL228) JE5780(=SP6) CROSS Hfr Ilv+ Leu+ (sup-126 trpE(Am) tyr(Am)) not tested JE5720 64-42 F- trp str dacB64 ts64 thr leu thi argH lac JE5599(=P4X8) JE10064 CROSS dacB64 His+ MetB+ JE5724 AGLS8 F- str argG leuS xyl JE5721 KL231(=JE5452) CROSS argG Thy+ Trp+ PurE+ JE5725 HFI-4 L+ Hfr F HfrP4X8(lac <- proA) thr ftsI730 thi? W3110 JE6602 LeuA+ JE5729 1a#7 F- dacB12 leu? mtl xyl malA spc dap lys DE(lac)72 dacA1191 JE5607 JE5652 CROSS Thi+ Met+ Ile+ SpcR Supplement 50_micro_g/ml DAP JE5730 ml Hfr F HfrC(purE -> lip) malA DE(lac)72 metB proA JE10085(=85.21) ME6085(=CD4) MetD+ Isogenic ponB85.21 = JE5637 JE5732 #21 OV4 F- dacB12 str rodA(SP6) aroB JE5713 JE5706 CROSS rodA(sp6) Lac+ StrR CephaloridineS dacA+?(ponA sup-126 trpE(am) tyr(am)) not tested JE5735 OV1 1a+ 14 F- ile? trp? gal? thi? met? ara? leu? lys dap spc xyl dacB12 rodA(SP6) mtl? JE5711 JE5688 CROSS ponA+ SpcR ArgG+ His+ PurE+ Supplement 50_micro_g/ml DAP JE5739 JE5615/1a+#3 F+ F Other dacB12 tonA metB proA str DE(lac) dacA1191 ponB353 ponAts1104 tsx lys dap JA200/PLC29-47 JE5615 CROSS PLC29-47 TR Sm Supplement 50_micro_g/ml DAP JE5740 JE5615/1b+#3 F+ F Other str ponAts1104 ponB353 dacA1191 dacB12 dap lys tsx DE(lac) tonA metB proA JA200/pLC19-19 JE5615 CROSS pLC19-19 TR StrR JE5742 OV3/1a+ F+ F Other str ponAts1104 rodAsp6 dacB12 dap lys metB Eam? trp sup-126? JA200/pLC29-47 JE5733 CROSS pLC29-47 ColE1R StrR Supplement 50_micro_g/ml DAP JE5743 OV3/1b+3 F+ F Other str lys dap dacB12 rodAsp6 ponAts1104 Eam? trp sup-126? metB JA200/pLC19-19 JE5733 CROSS pLC19-19 ColE1R StrR Supplement 50_micro_g/ml DAP JE5744 JE5733/1b+3.LA.3 F+ F Other dacB12 Eam? trp sup-126? lys dap str leuA(thr thi) rodAsp6 ponAts1104 JE6602 JE5743 CROSS leuA MetB+ StrR Supplement 50_micro_g/ml DAP JE5751 1104-1 F- dacB12 malA str xyl ponAts1104 dacA1191 JE5604 JE5605 ponAts1104 AroB+ JE5756 #24 F- DE(lac) tonA metB aroB ponB353 leuA dacB12 dacA1191 str JE5752 JE5605 CROSS ponB353 leuA Xyl+ StrR No growth on LA-NaCl at 30C JE5762 F- DE(lac) str tonA metB dacA1191 dacB12 ponB353 aroB JE5623 JE5756 LeuA+ ftsI+ isogenic strain of JE5760 & JE5761 JE5766 5755L+3-L1 F- aroB dacB12 str dacA1191 JE5623 JE5755 LeuA+ plS ftsI+ isogenic strain of JE5765 JE5774 MI183e F- xyl leuB ilvH611 thi-1 metE proC purE trp lysA lacZ str tonA tsx M. Iaccarino Supplement 40_micro_g/ml Ade 29929 JE5778 JEY164 F- nalA str arg leu proB lacZ Y. NISHIMURA JE5780 SP6, B6 F- ponA tyr(am) trpE9829(Am) ilv sup-126 rodA pBC1a-, pBC2-, ovoid, ts 29786 JE5785 704 dapD20 F- thi purE leu str ara mtl xyl gal tonA trp his argG ile metA or B JE5731 JE5481 DapD+ ponB+ isogenic strain of JE5784 JE5790 1191 DL-27 F- lys dacA1191 ts1191 thr leu thi argH his dap lacY mtl xyl malA str tonA JE5707 JE11191 CROSS dap lys Thy+ X+ StrR Supplement 50_micro_g/ml DAP JE5791 1191 DL-16 F- his dap lys lacY mtl xyl malA str tonA ts1191 thr leu thi argH JE5707 JE11191 CROSS dap lys Thy+ X+ StrR Supplement 50_micro_g/ml DAP JE5792 1191 DL-28 F- argH his dap lys mtl xyl malA str tonA thi leu thr ts1191 dacA1191 JE5707 JE11191 CROSS dap lys Thy+ X+ StrR Supplement 50_micro_g/ml DAP JE5793 JE4 P1 F+ F lac acrA str JE5664 JE4 PurE+ JE5795 PuG81/F13 F+ F mtl ile thi metA or B ara trp tsx proC lacY leu dacA1191 argG his str xyl W3747 JE5767 CROSS F13 Lac+ StrR DacA- JE5800 Pr33 Hfr F HfrP4X8(lac <- proA) leu metB thi ile mtl xyl str argG his trp purE gal ara JE5664 JE5597 CROSS ProC+ StrR JE5801 r11 F- lys man aroD pps dap thi argH his str lacY mtl xyl malA JE5516 SPONTANEOUS Phenyl mercury acetateR (PMA1mg/ml and cross-streak) Supplement 50_micro_g/ml DAP JE5802 MFT1033 Arg+ FtsD+#6 F- lacY malA mtl xyl mel str lpm thr leu thi trp lys dap cys ilv phe tyr W3110 MFT1033 ftsD+ ArgH+ JE5803 MFT1033 Arg+ FtsD#4 F- trp cys ilv phe tyr dap lys lacY malA mtl cyl mel str leu thi thr W3110 MFT1033 (lpp1 or lpm) ArgH+ ftsD103(TS) 1033 JE5804 #1 F- trp lpm leu lys dap cys ilv lacY JE5596 MFT1033 CROSS Xyl+ FtsD+ ArgH+ Thi+ Thr+ Leu+ His+ Supplement 50_micro_g/ml DAP JE5805 #2 F- leu lpm lacY ilv lys trp JE5596 MFT1033 CROSS Xyl+ FtsD+ ArgH+ Thi+ Thr+ Leu+ His+ JE5811 DL-6 F- lac lys lpm? JE5596 MFT1033 CROSS Xyl+ FtsD+ His+ ArgH+ Thi+ Thr+ Leu+ JE5812 JE346trp+ F- λ+ ind- str tsx phoA rha pil mal1 mtl ara2 xyl2 lac85 purE gal2 JE346 SPONTANEOUS Trp+ revertant JE5813 #1 F- phoA purE lac-85 xyl-2 ara-2 mtl mal-1 str tsx rha pil (lambda)- JE5528 JE5812 CROSS Gal+ StrR JE5814 #15 F- lac-85 xyl-2 ara-2 mtl mal-1 str tsx pho rha pil (lambda)- JE5528 JE5812 CROSS Gal+ StrR JE5815 #29 F- pil trp tsx purE lac85 xyl2 ara2 mtl mal1 str lambda_- pho rha JE5528 JE5812 CROSS Gal+ StrR JE5816 #40 F- lambda_- xyl2 ara2 mtl mal1 str rha pil JE5528 JE5812 CROSS Gal+ StrR JE5817 #6 F- λ+ lambda_ind- purE pil rha phoA tsx str mal-1 mtl ara-2 xyl-2 lac-85 JE5528 JE5812 CROSS Gal+ StrR JE5818 D1 Hfr F HfrG6(metC <- argG) mel str his xyl mtl malA argH trp thi thr ftsD JE5596 MFT1033 CROSS Lac+ Lys+ StrR JE5821 T1 F- tyr lpm ftsD his thr leu thi argH lys dap cys ilv phe lacY malA mtl xyl mel str JE5596 MFT1033 CROSS Trp+ StrR JE5822 T2 F- malA mtl ilv ftsD his thr thi argH lys dap cys xyl phe mel str tyr lacY JE5596 MFT1033 CROSS Trp+ StrR Supplement 50_micro_g/ml DAP JE5824 22 F- xyl ftsD phe tyr lacY lpm malA dap lys argH thi str cys thr mel mtl JE5596 MFT1033 CROSS Trp+ StrR Supplement 50_micro_g/ml DAP JE5825 23 F- malA thi argH lys dap cys ilv phe tyr lacY thr mtl xyl mel str lpm ftsD JE5596 MFT1033 CROSS Trp+ StrR Supplement 50_micro_g/ml DAP JE5826 KT130 F- rif sup-126 thr trpE9826(Am) tyr(am) thy his aroC(?) dapE9 T. Nagata JE5827 Dap-2 F- trpE(Am) tyr(am) thy dapE9 rif thr JE5528 JE5826 CROSS AroC+ MalB+ RifR Supplement 50_micro_g/ml DAP JE5829 Dap-His-4 F- tyr(am) trpE(Am) thr sup-126 his dapE9 rif thy JE5528 JE5826 CROSS AroC+ MalB+ RifR JE5831 KT130 NalA F- thy thr trpE9826(Am) tyr(am) his aro(?) dapE9 rif sup-126 nalA JE5594 JE5826 nalA NalR(50_micro_g/ml) JE5845 K27 F- fadD88 N. Otsuji 29952 JE5859 17-16 F- malA mtl xyl lacY str thi argH his lys dap pps man JE5502 JE5516 AroD+ Supplement 50_micro_g/ml DAP JE5860 17-17 F- man argH pps lpm thi str lacY xyl mtl malA his lys dap JE5502 JE5516 lpm AroD+ Supplement 50_micro_g/ml DAP JE5870 ma20 Hfr F HfrC(leuS <- purE) JE5514 JE5504 Man+AroD+ JE5879 p17 Hfr F HfrC(purE -> lip) man JE5514 JE5504 Pts+ JE5896 r13 F- lacY man aroD pps dap lys thi argH his str mtl xyl malA JE5516 SPONTANEOUS Phenyl mercury acetateR (PMA1mg/ml and cross-streak) Supplement 50_micro_g/ml DAP JE5899 K63m1fadD+ F+ F gal str lpm his W3110 JE5538 fadD+ FadD+ JE5900 K63m2fadD+ F+ F his gal str W3110 JE5539 fadD+ FadD+ JE5901 17-8arg+1 F- xyl mtl malA pps man dap lys his thi str lacY W3110 JE5509 argH+ ArgH+ Supplement 50_micro_g/ml DAP JE5902 17-15arg+2 F- pps dap lys his thi str lacY mtl xyl malA man lpm W3110 JE5858 argH+ Arg+ Supplement 50_micro_g/ml DAP JE5903 r11-16arg+4 F- dap pps lpo malA xyl mtl man lacY str thi his lys W3110 JE5510 argH+ Arg+ Supplement 50_micro_g/ml DAP JE5935 HfrC mgp125 Hfr F HfrC(leuS <- purE) uidA pps man JE5591 JE5504 uidA AroD+ JE5939 Lpo Rec#17 F- man str thi xyl malA? mtl? recA1 his pps lpo JE5594 JE5510 CROSS recA1 Lys+ Dap+ ArgH+ StrR JE5940 HfrC map3 x LC102#4 F- gal argG his aroD pps man str JE5504 JE5597 CROSS man pps aroD PurE+ ProC+ Leu+ Thi+ Met+ Ile+ Trp+ StrR JE5942 R11-16 Thy F- thy malA mtl xyl lac str thi argH his lys dap pps man lpo JE5510 SPONTANEOUS thy TrimethoprimR Supplement 50_micro_g/ml DAP JE5948 AO2,2 F- uidAl aroD argEH gal xyl lac mtl str spc JE5517 CURING/AO Aro- JE5951 AO3 F- str gal trp pps lpm man JE5950 CURING/AO Man- JE5964 5 F- metC gal lac dacB12 mtl malA thy pps nalA JE5953 JE5787 Man+ AroD+ JE5975 TrMP5 F- rif str thy galK2 JE5664 JE5585 CROSS Trp+ RifR JE5977 MPH19 F- rif galK2 str thy his trp JE5664 JE5585 CROSS Man+ RifR JE5980 ThS6 F- trp man lpm pps rif galK2 JE5664 JE5585 CROSS Thy+ RifR JE5984 KL253/F2-gal F+ F str-118 malA1 thi-1 pyrD34 his-68 trp-45 tyrA2 recA1 mtl-2 xyl-7 galK35 B. J. Bachmann 29912, 29906, 29942 JE5990 64DL1 F- str tonA dacB64 ts64 dap lys thr leu thi argH trp lacY mtl xyl malA JE5707 JE10064 CROSS dap lys Thy+ StrR Supplement 50_micro_g/ml DAP JE6602 HFI-4 Hfr F HfrP4X(argF -> lac) thr (thi) ftsI leuA Nishimura 730-60' JE6183(=CV530C) CROSS ftsI Leu+ JE6603 FI7301 F- ftsI thi DE(pro-lac) Nishimura 730AB JE6183 ftsI Leu+ JE6604 PC-1237=TKL-11 F- tonA tsx phx supE ths dra sus uvrB vtr murE thr leu thi pyrF codA thyA argG ilvA his lacY PC 0417 E. J. J. Lugtenberg no growth/LB+-NaCl at 41C 29899, 29893 JE6605 MURE 3703 F- thi DE(pro-lac) murE PC1237 CV 530 C murE leuA+ salt resque type ts, complete ts in DNB JE6607 MURF 4206 F- thi murF DE(pro-lac) PC1242=JE6606 JE6183 murF leu+ JE6611 MRA 2201 F- murC DE(pro-lac) thi ST222=JE6610 JE6183 mra Leu+ JE6613 MRA 6401 F- ddl DE(pro-lac) thi ST640=JE6612 JE6183 mra Leu+ JE6617 FA 8401 F- ftsZ84 thi DE(pro-lac) LC605T841 JE6183 ftsZ Leu+ JE6622 FI 3301 F- thi ftsI33 DE(pro-lac) JE5651 JE6183 ftsI Leu+ JE6624 MURF 4221 F- thi murF DE(pro-lac) thy MURF4206 OTHER METHODS (TMP) thy JE6631 Hfr F Hfr? (thi) str polA1 P4X8 JE6268 polA1 AA+ Lac+ JE6637 JE6268 Rif#1 F- thi purE trp lys proC leu lacZ xyl ara mtl mal polA1 tonA tsx str rif (man) (gal) (mel) E5014 Rif JE6268 rif RifR high background of TD(spontaneous?) JE6638 JE6268 rif nal#1 F- purE trp lys proC leu thi nalA rif str tsx tonA polA1 (mel) (gal) (man) mal mtl lacZ ara xyl Lin623 JE6268 rif#1 nalA NalAR high background of TD(spontaneous?) JE6642 JRG902 F- nalA39 menC1 trpA9761 (lambda)- gal-25 trpR72 iclR7 rpsL195 menC: no growth on minimal glycerol fumarate with anaerobic 29738 JE6647 JE6268 rif nal ftsI F- tsx thi proC lys trp purE xyl ara mtl mal (man) (gal) (mel) ftsI730 polA1 tonA lacZ str rif nalA FI7301=JE6603 JE6268 rif nal #1=JE6638 ftsI Leu+ Supplement 40_micro_g/ml Ade JE6649 C600 Sm F- tonA thr leu thi lacY supE str C600 StrR JE6651 C600 Sm recA#1 F- str thi lacY tonA supE thr recA1 leu LC248 C600 Sm thy #1=JE6650 CROSS recA Thy+ 862 JE6653 CR63 F- (su1) supD prototroph Takeda JE6654 ER F+ F asnB32 thi-1 relA1? lambda_- asnA31 Meyenberg -> Bachmann -> Takeda. 78.12.14 revision F+ 79.5.15 revision asnA,B asn100_micro_g/ml 29960, 29750 JE6655 K-1100 F- endA HfrC W208(F- thr leu) A. Sugiura 29986 JE6656 AX621 F- proA arg thi leu thr ftsA1882 his mtl ara xyl gal rpsL lac J. R. Walker (78.8:3) 29747 JE6657 AX732 F- rpsL nadC thr sep-2158 J. R. Walker JE6658 TKF10 F- thr thi pyrF thyA lac tonA ftsA10 leu H. Wijsman -> J. R. Walker 29747 JE6659 KL 266 F- leu6 thi-1 metE70 proC32 malA38 hisF860 thyA54 cysC43 lacZ36 ara-14 mtl-1 xyl-5 str109 spc15 JE6660 UH-Ac2 F+? F mel-1 supF58? aceE2 trp-26 U. Henning -> Bachmann 30011 JE6661 JRG596 Hfr F HfrH(valS <- uxuAB) thyA56 metB1 azi-14 ton-54 tsx-87? DE73 pps-1 relA1 J. R. Guest -> Bachmann DE73 = DE(aroP-aceF) apparently no longer an Hfr 29739 JE6662 EWH193 F- thyA3 argG6 metJ107 rpoB306 lacY1 gal-6 malA1 supE44? rpsL-8,9,104 tsx-64 hisG1 metK110 E. W. Hafner -> Bachmann 29744 JE6665 B78A F- lacY1 leuB6 thi galR61 strA129 supE44 galR61 is a constitutive mutation 30176 JE6672 Lin201 Hfr F HfrPO2A of C(purE -> lip fep) glpA25 glpD10 relA1 phoA8 tonA22 T2R pit-10 soiT1 phoA8 = DE10 JE6673 Lin206 Hfr F HfrPO2A of C(purE -> lip fep) tonA22 pit-10 spoT1 T2R relA1 phoA8 glpA9 Bachmann phoA8(=DE10) 29967 JE6674 Lin402 Hfr F HfrPO2A of C(purE -> lip fep) spoT1 phoA8 glpA9 relA1 DE14 pit-10 T2R tonA22 E. CC Lin -> Bachmann phoA8(=DE10) DE14(=glpR-malA) 29967 JE6800 12 td1 F- trp dacB12 thr leu thi argH thy his lacY mtl xyl malA str tonA W3110 JE10012 TR JE6802 12 DL2 F- trp dap lys lacY mtl xyl malA str tonA dacB12 ts12 thr leu thi argH his JE5707 JE10012 CROSS dap lys Thy+ StrR Supplement 50_micro_g/ml DAP JE6809 ilv66 F- metB malA str his gal tonA ara thi argH tsx lac mtl JE5662 JE5436 CROSS IlvA+ StrR JE6810 Met-3 F- str tonA ara thi argH ilvA mtl xyl malA his gal tsx lac JE5662 JE5436 CROSS MetB+ StrR JE6811 ilv1 F- str his gal tsx lac tonA ara thi argH metB mtl xyl malA JE5662 JE5436 CROSS IlvA+ StrR JE6813 Xyl-5 F- lac his gal str tonA ara thi argH ilvA mtl malA tsx JE5662 JE5436 CROSS Xyl+ StrR JE6814 Met-1 F- malA tonA ara thi ilvA mtl xyl str his gal tsx lac JE5662 JE5436 CROSS MetB+ StrR JE6816 Trp-Lac23 Hfr F HfrP4X8(argF -> lac) thi tonA str malA xyl mtl his thy argH thr leu JE5662 PA3092 CROSS Trp+ Lac+ StrR JE6820 Lac15 Hfr F HfrP4X8(argF -> lac) str thy his trp mtl malA JE5662 PA3092 CROSS Lac+ StrR JE6839 T4 F- gal str malA mtl his metC argG lac tsx JE5662 JE5450 CROSS Thy+StrR JE6846 12G22 F- dacB12 aspB str JE5662 JE5775 dacB12 ArgG+ cf. dacB+ isogenic strain = JE6847 JE6847 12G23 F- aspB str JE5662 JE5775 ArgG+ dacB+ isogenic strain of JE6846 JE6850 S12 F- his metC dacB12 gal nalA lac mtl JE6364 JE5661 ArgG+ JE6851 S13 F- metC lac gal his nalA mtl JE6364 JE5661 dacB+ ArgG+ JE6852 R1 F- gal mtl argR metC nalA his lac JE6364 JE5661 argR dacB+ ArgG+ JE6855 KLF41 AO1 F- metC dacB12 mtl malA str thy nalA his gal lac JE6853 CURING/AO Mal- JE6866 AB14 DL2 Hfr F HfrP4X8(lac <- proA) malA str his dacA1191 lys dap dacB64 JE5707 JE6864 CROSS dap lys Thy+StrR Supplement 50_micro_g/ml DAP JE6868 AB14 DL6 Hfr F HfrP4X8(lac <- proA) malA dacB64 dacA1191 dap lys str JE5707 JE6864 CROSS dap lys Thy+StrR Supplement 50_micro_g/ml DAP JE6884 LacM31 F- xyl mtl JE5599 JE10980 CROSS Lac+ MetB+ JE6885 LacM16 F- str xyl malA ponA980 mtl JE5599 JE10980 CROSS Lac+ MetB+ JE6889 PA505 MS11 XM F- argH str metA asd W3110 JE6347 mtl+ Xyl+ asd : requires dap JE6890 Asd67 F- str metA argH malA JE5607 JE6889 CROSS Asd+ StrR JE6892 Asd27 F- argH metA str JE5607 JE6889 CROSS Asd+ StrR JE6893 Asd64 F- malA metA argH xyl str JE5607 JE6889 CROSS Asd+ StrR JE6895 Asd8 F- mtl xyl argH metA str JE5607 JE6889 CROSS Asd+ StrR JE6912 D31 F- lys his pps aroD man thi argH mtl xyl malA str JE5607 JE5516 CROSS Dap+ StrR JE6913 D67 F- thi pps malA lys aroD man str xyl mtl argH JE5607 JE5516 CROSS Dap+ StrR JE6914 D68 F- mtl thi argH xyl malA str lys JE5607 JE5516 CROSS Dap+ StrR JE6922 980C2 F- str lysA argA his mtl malA mal-18? gal-6? supE JE5607 JE5447 CysG+ JE6923 980C3 F- gal-6? str supE mal-18? malA mtl his argA lysA ponA980 JE5607 JE5447 ponA980 CysG+ JE6924 980C7 F- malA mtl lysA his gal-6? argA supE mal-18? JE5607 JE5447 CysG+ ponA+ isogenic strain of JE5665 JE6925 C11-9 F- ponA980 his mtl gal-6? supE argA lysA JE5435 JE5665 Mal+ JE6926 C11-10 F- supE lysA argA his mtl gal-6? JE5435 JE5665 ponA+ Mal+ JE6927 C11-15 F- mtl gal-6? supE ponA980 lysA argA aroB his JE5435 JE5665 Mal+ JE6928 C11-17 F- gal-6? supE lysA argA aroB his mtl JE5435 JE5665 ponA+ Mal+ JE6929 151 td1 F- argH thy his trp lacY mtl xyl malA str tonA thi leu thr rodA151 ponA151 JE5453 JE10151 TR JE6971 Py22 F- str purC his thi argH mal JE6898 JE5455 CROSS PyrB+ Thy+ JE6973 Py3 F- str purC his valS? thi mal JE6898 JE5455 CROSS PyrB+ Thy+ JE6976 Py37 F- str his thr valS? ponBS980 leu thi argH mal purC JE6898 JE5455 CROSS PyrB+ Thy+ JE6978 704 DL10 F- lys thr ts704 ponB704 dap argH thi leu xyl malA str tonA mtl lacY trp his JE5707 JE10704 CROSS dap lys Thy+ StrR Supplement 50_micro_g/ml DAP JE6987 704 X224 Hfr F HfrP4X8(lac <- proA) str dacA1191 ts thr leu thi argH lys thy his trp malA ponB704? JE6866 JE10704 CROSS Xyl+ Trp+ Dap+ JE6989 704X213 Hfr F HfrP4X8(argF -> lac) str ponB704? dacA1191 dacB64 ts thr leu thi argH lys his malA JE6866 JE10704 CROSS dacB64 dacA1191 Xyl+ Trp+ Dap+ JE6990 704X214 Hfr F HfrP4X8(argF -> lac) his lys str mal dacA1191 dacB64 ts thi argH JE6866 JE10704 CROSS dacB64 dacA1191 Xyl+ Trp+ Dap+ JE6991 704XT211 F- lys thy his mal lac ponB704 dacB64 ts thr leu thi argH trp str JE6866 JE10704 CROSS Xyl+ Thy+ Trp+ Dap+ JE7012 151male30 Hfr F HfrP4X8(lac <- proA) rodA151 trp argH JE5599 JE10151 CROSS Hfr Lac+ MetB+ MeeillinamR, TS on LA-NaCl JE7015 1116P101 F- rodAts1116 lacY str thi argH his trp mtl xyl malA JE5599 JE11116 CROSS PlS Thr+ Leu+ Thy+ StrR MetB+ JE7018 157P13 F- rodA157 lacY str thi argH his trp mtl xyl malA JE5599 JE10157 CROSS PlS Thr+ Leu+ Thy+ StrR Met+ JE7023 KLF1/AB2463 F+ F supE44 recA13 lacY1 galK2 mtl-1 xyl-5 argE3 ara-14 str-31 tsx-33 his-4 proA2 thi-1 leu-6 thr-1 B. J. Bachmann 29906, 29942, 29912 JE7025 KLF1 AO F- thi argH thy his trp lacY mtl tonA str malA xyl ftsI730 thr leu JE7024 CURING/AO Thr-Leu- JE7036 730 rev1 F- trp lacY mtl xyl malA str tonA thr leu thi argH thy his JE10730 SPONTANEOUS TR PenGR at 30C, PenG+ at 42C JE7057 Pu6 AG1 dnaB F- ara leu tsx gal his str thi dacB12 dacA1191 dnaB(T43) proC lac trp xyl mtl ile JE5361 JE5630 CROSS dnaB(T43) Met+ StrR JE7058 Pu6 AG1 dnaB/pLC26-6 F+ F Other leu dnaB(T43) dacA1191 dacB12 thi str his gal tsx ara ile mtl xyl trp proC lac JA200/pLC26-6 JE7056 CROSS pLC26-6 ColE1R StrR JE7083 353-11aroB F-? aroB dacB12? tsx DE(lac) tonA metB str ponB353 JE5435 JE7064 aroB Mal+ JE7090 AGLS8 thy#26 F- xyl leuS thy str argG JE5724 SPONTANEOUS thy TrimethoprimR JE7091 #26-21 F- str xyl argG leuS lys dap JE5707 JE7090 CROSS dap lys Thy+ StrR JE7093 26-21 TR24 F- str lys argG xyl dap JE5453 JE7091 LeuS+ JE7094 26-21 tr24#52 F- lys malA metB metD proA DE(lac) tsx argG dap str JE6085 JE7093 CROSS metD malA Xyl+ StrR JE7313 JE5757 1b+ DapD2 F- metB ponAts1104 dacA1191 dacB12 dapD leuA str DE(lac) JE5481 JE5757 dapD PonB+ (Cephaloridine 1_micro_g/mlR) JE7315 JE5760 1b+ dapD7 F- DE(lac) ftsI730 metB dacA1191 dacB12 dapD aroB str JE5481 JE5760 dapD PonB+ (Cephaloridine 1_micro_g/mlR) JE7325 F- thyA argH thi lacY malA mtl xyl tonA supE str trp leu thr his JE10911 SPONTANEOUS TR 3234 JE7334 F- str T1R T5R trp purE leu lys metE thi sup(Am SU+) ara lacY xyl JE7332 AN105 proC+ JE7336 F- lacZ str T1+ T5+ sup(Am SU+) tgt trp lys purE leu metE thi ara xyl JE7332 AN105 proC+ JE7338 F+ F str lac purE tsx JE7332 JE4 acrA+ JE7339 F+ F tsx purE tgt str lac JE7332 JE4 acrA+ JE7344 ? ara ileS proC galK trp his argG xyl mtl metA or B tgt JE7331 LC102 CROSS PurE+ ThyA+ JE7345 ? mtl metA or B ileS ara proC galK trp his argG xyl JE7331 LC102 CROSS PurE+ ThyA+ JE7348 ? xyl mtl thi ara leu azi tonA lacY entA trp str JE7330 AN193 CROSS ProC+ His+ ThyA+ JE7349 ? tgt ara leu azi tonA lacY entA trp str xyl mtl thi JE7330 AN193 CROSS ProC+ His+ ThyA+ JE7403 AB47-18 F- (lambda)- sup-37 supE44 tsx-33 str-31 mtl-1 xyl-5 ara-14 galK2 lacY1 argE3 his-4 proA2 thi-1 leu-6 thr-1 ts ponB pfv(PBP-5-) James T. Park(Prof.) parent = AB1157 ts(salt effect+ filament), PlS mucoid colony, Cephaloridine-hypersensitive JE7404 lip+AB#4 F- xyl-5 thi-1 his-4 proA2 purB15 mtl-1 galK2 lacY1 str-35 supE44 JE7403 JE5453 Lip+ cf. PBP-5- isogenic = JE7401 & JE7402 penG MIC = 20_micro_g/ml, TR, PlS JE7410 JE7092 Lip+#3 F- argG lys dap str xyl JE5663 JE7092 Lip+ P1S cf. dacA- isogenic = JE7411 JE7411 JE7092 lip+#47 F- argG lys dap dacA1191 str xyl JE5663 JE7092 dacA1191 Lip+ PenGS, P1S cf. dacA+ isogenic = JE7410 Supplement 50_micro_g/ml DAP JE7418 7092 lip+#9 F- argG xyl str pfv dap lys JE7403 JE7092 pfv Lip+ Supplement 50_micro_g/ml DAP JE7419 7418 argG+#1 F- dap lys str xyl pfv JE5662 JE7418 ArgG+ cf. dacB12- isogenic = JE7421 & 7422 Supplement 50_micro_g/ml DAP JE7421 7148 argG+#3 F- pfv lys str xyl dacB12 dap JE5662 JE7418 dacB12 ArgG+ cf. dacB+ isogenic = JE7419 & 7420 Supplement 50_micro_g/ml DAP JE7439 SP6 trAB#5 F- trpE(Am) sup-126 tyr(am) ponA ilv JE7403 JE5780 RodA+ JE7440 SP6 trAB#6 F- ilv pfv sup-126 trpE(Am) tyr(am) ponA JE7403 JE5780 pfv RodA+ JE7445 ls1#10 F- thyA deoC str JE7403 JE5452 LeuS+ JE7456 4+ F- argG xyl str JE5693 JE5724 LeuS+ JE7474 6-L4 Hfr F HfrP4X8(argF -> lac) str spc lys psx+- his trp gal dap pro leu ara thi? mtl JE5711 JE7572 CROSS Lac+ SpcR PurE+ JE7484 MFT1181F+ pLC26-6#3 F+ F Other thr leu thi argH thy his trp lacY mtl xyl malA str tonA ftsE JA200/pLC26-6 MFT1181 CROSS pLC26-6 ColE1i StrR JE7488 CD4-m3 Hfr F HfrC(purE -> lip) tsx DE(lac) ponB proA metB malA JE7403 JE6085 ponB MetD+ JE7495 EntA+5#14 F- azi tonA tsx thi leu proC supE44 trpE mtl xyl lacY ara str JE7403 JE7493 EntA+ JE7518 Lip+ LeuS- 2 F- thi his proA purB mtl xyl galK lacY str supE leuS JE5724 JE5453 leuS Lip+ JE7535 EM3003 Mal+ 1 F- thi lac purF aroC arg dsdA7 str W3110 JE6329 Mal+ JE7539 6 F- thi lac str arg JE6983 JE7535 CROSS AroC+ MalA+ JE7542 41 F- str thi arg lys pbpS704 lac JE6983 JE7535 CROSS AroC+ MalA+ JE7543 46 F- arg lac thi lys str JE6983 JE7535 CROSS AroC+ MalA+ JE7544 8 F- 704 PBP-S1 lys thi arg str lac his JE6983 JE7535 CROSS AroC+ MalA+ JE7545 10 F- PBP-S1 arg thi lys his 704 lac str JE6983 JE7535 CROSS AroC+ MalA+ JE7547 4+ F- trp his nalA JE7540 JE7546 PurF+ JE7549 1 F- aroC nalA trp his JE7540 JE7546 PurF+ JE7551 E101 Nal-82 F- leu-6 nrdA thr-1 tonA21 nalA lacY1 thi-1 supE44 str JE5778 E101 NalR JE7559 4 F- dcd mtl gal tpp str his argG nrdB nalA ftsI cdd metB leu lacY or Z malA xyl JE7556 JE6333 NalR JE7564 JE6983 nal1 Hfr F HfrP4X8(lac <- proA) his ts704 dacB64 nalA str malA mtl thi? PBP-S1 704 dacA1191 argH lys JE5778 JE6983 NalR JE7565 JM477 F- str gnd kga ptsF leu kga(=eda) 29874 JE7566 21 F- nalA PBP-S1 gnd eda str lys 704 JE7564 JE7565 CROSS PtsF+ ArgH+ JE7567 27 F- gnd eda str lys nalA JE7564 JE7565 CROSS PtsF+ ArgH+ JE7569 30 F- str gnd eda JE7564 JE7565 CROSS PtsF+ ArgH+ JE7570 12 F- his str PBP-S1704 JE7564 JE7565 CROSS PtsF+ ArgH+ JE7571 10 F- str his JE7564 JE7565 CROSS PtsF+ ArgH+ JE7572 LS174 F- dap sup(Am SU3.A81 TS) lac str psx+- rodA pro his trp lys J. T. Park JE7573 JE6347 MalA F- metA str malA mtl xyl asd argA JE6347 SPONTANEOUS malA lambda_VR asd : requires dap JE7574 JE6927 MalA F- argA malA ponA980 aroB lysA his mtl gal-6? supE44 JE6927 SPONTANEOUS malA lambda_VR JE7584 td2 Hfr F HfrP4X8(argF -> lac) nadC ilvH leu thi thr JE5774 JE7476 Sep+ JE7598 MI183eL+730#2 F- xyl lys trp ftsI730 proC metE thi purE tsx tonA str lacZ JE5725 JE5774 ftsI730 Leu+ Supplement 40_micro_g/ml Ade JE7600 MI183eL+730#5 F- thi ilvH611 metE proC purE trp lys xyl lacZ str tonA tsx JE5725 JE5774 Leu+ JE7601 MI183eL+730#6 F- lacZ str tonA tsx thi metE proC purE trp lys gal xyl JE5725 JE5774 Leu+ JE7602 MI183eL+33#1 F- tsx str ftsI tonA thi metE proC purE trp lys xyl lacZ JE5651 JE5774 ftsI Leu+ Supplement 40_micro_g/ml Ade ftsI(MFT33) JE7604 MI183eL+33#4 F- lys thi metE proC purE trp gal- xyl lacZ str tonA tsx JE5651 JE5774 Leu+ Supplement 40_micro_g/ml Ade JE7605 MI183eL+33#6 F- str tonA tsx ilvH611 thi metE proC purE trp lys xyl lacZ JE5651 JE5774 Leu+ Supplement 40_micro_g/ml Ade JE7606 MI183eL+AX#10 F- lys tonA tsx lacZ xyl str trp purE proC metE thi JE7480 JE5774 Leu+ Supplement 40_micro_g/ml Ade JE7607 MI183eL+AX#12 F- sep2158 tonA str lacZ xyl tsx gal- lys trp purE proC metE thi JE7480 JE5774 sep2158 Leu+ Supplement 40_micro_g/ml Ade JE7608 MI183eL+730#12 F- str thi metE proC purE trp lys xyl lacZ tonA tsx JE5725 JE5774 Leu+ Supplement 40_micro_g/ml Ade JE7609 MI183eL+AX#11 F- lacZ xyl lys trp purE proC metE thi sep2158 tsx tonA str JE7480 JE5774 sep2158 Leu+ Supplement 40_micro_g/ml Ade JE7614 R32 F- mtl trp str ts top-10 lacY malA xyl thy his argH thi? leu thr P4X8 JE10010 CROSS TR TR StrR Rolf Sternglanz TS on L-agar (-NaCl) JE7616 6111male Hfr F HfrG6(metC <- argG) asnA asnB thi? str JE5596 JE6274 CROSS Hfr Thy+ His+ JE7617 #21 F- xyl rodA sp6 spc dap lys leu? ara? met? thi? ile? trp? gal? JE5711 JE5688 ponA+ SpcRArgG+His+PurE+ Supplement 50_micro_g/ml DAP JE7618 LM49 Hfr F HfrP4X8(Lac <- proA) thy his trp top-250? mtl xyl malA str JE5599 JE7626 CROSS Lac+ Met+ JE7623 LM42 Hfr F HfrP4X8(argF -> lac) his top-250? leu thr argH malA JE5599 JE7626 CROSS Lac+ Met+ JE7626 IL21 F- his trp lacY mtl xyl malA str tonA ts thy argH thi leu thr top-250 ? JE10250 TR Rolf Sternglanz TS on L-agar (-NaCl) JE7627 F- tsx-76? lac-3 proA3 leuA371 ilvH611 metB1 malA38 lysA dapA tonA2 dacB12 ponB704 str JE5784 JE7657 ponB704 MetD+ Supplement 50_micro_g/ml DAP JE7636 MA12 F- thi mtl xyl serA str nalA his trp galK lacY tonA? thr? glc? metK? proA leu ara JE7631 JE7561 CROSS MetG+ ArgH+ JE8471 IO-7012 ilv DE(cya)854 his trp J. Beckwith -> Y. Kaziro -> Y. Takabe 35444 JE8472 GP11 crpS metE ilv cya Y. Takebe JE8473 GP11 SpecR spc cya ilv metE crpS JE6082 GP11 SpecR JE8474 SM32 F- rpsL gal DE(lon-100) pyrD his 30054 JE8476 LC158 F- arg lac gal ara xyl mtl T6R lambda_S str lon thi met his pro leu thr AB1899 non-mucoid variant derived from AB1899 30174 JE8478 GY6130 F- thi thyA36 dra-34 ura-49 supE lacY1 tonA101 lexB30 thr-4 leu-8 lexB30(=recA430) 864 JE8479 QR140(=AB2494) F- thi? metB thr leu his pro gal xyl str lexA1 Hideyuki Ogawa 864 JE8480 DM1187 F- strA31 tif-1 gal lac pro spr-51 lexA3 sfiA11 leu thr his-4 Hideyuki Ogawa JE8481 Rec+ Clark -> Grete Kellenberger JE8482 RecB21 Clark -> Grete Kellenberger UV test OK(1982.1.18) JE8483 RecC22 Clark -> Grete Kellenberger UV test OK(1982.1.18) JE8484 LC607 RecA F- trp purE lys metE proC leu thi lacZ xyl ara tsx tonA str (lambda)- lambda_S recA JE8486 LC250 Hfr F HfrKL16-99(thyA -> serA) thi nalR recA1 (lambda)- JE8487 female3- phe- (rec+) female3 phe JE8488 female3 recA1 JE8489 Hfr HMS83 Hfr F HfrP4X8(argF -> lac) thy polB1 polA1 thi lys JE8491 JE6632 Hfr F HfrP4X(argF -> lac) (thi)? polA1 JE8492 W3623 trp gal str H. Ogawa -> Oka 29977 JE8494 H102616 F+ F endI polB1 str thyA thr leu polA1 JE8495 AX727 F- lac dnaZ str thi JE8497 K802 grpA80 lac str (dnaB) Haruo Saito 29741 JE8498 K1702 F- rpsL31 grpC170 leuB6 lacY1 supE44 rfbD1 thi-1 malT1(lambda_R) ara-14 galK2 xyl-5 mtl-1 mgl-51 kdgK51 DE(gpt-proA)62 hisG4(Oc) argE3 (lambda)- Rac- Haruo Saito grpC170(= dnaK): a little leaky Ts thr- -> thr+ JE5517 JE5515/F'm-p- F+ F aroD gal xyl lac mtl str spc uidA1 argEH JE5587 JE5515 CROSS AroD+ JE5521 P12 Hfr F HfrC(leuS <- purE) man lpo JE5514 JE5504 lpo Pps+ JE5527 lpo rec F- lpo thi xyl str lac? mtl? malA? pps man recA1 nalA his LC250(JE5594) JE5510 CROSS recA1 Lys+ Dap+ ArgH+ StrR JE5533 Gp1 Hfr F HfrC(leuS <- purE) lpm JE5502 JE5503 lpm UidA+ JE5544 G3 Hfr F HfrC(leuS <- purE) pps JE5502 JE5503 UidA+ 35657 JE5549 102X2.1 rec1/F'lpo+ m1.2 F+ F gal recA1 man lpm pps trp str JE5550 JE5525 CROSS F506 Man+ Arg+ JE5550 S343 rec/F'lpo+ F+ F nalA recA1 man aroD argEH gal xyl lac mtl str JE5554 JE5519 CROSS F'man+aroD+ Man+ AroD+ StrR F'lpo+ isolated by Dr. Y. Hirota JE5551 102X2.1 F- str gal trp pps lpm man JE5511 LC102(=JE5597) CROSS man lpm pps Leu+ Met+ Thi+ Ile+ StrR ArgG+ His+ purE+ proC+ JE5553 102X2.1 rec1/F'lpo#1 F+ F str lpm pps recA1 man trp gal JE5555 JE5525 CROSS F3033 Pps+ JE5554 KL208 Hfr F HfrKL208(man <- rac) rel-1? CGSC(B.Bachmann & M.Berlin) suppressor-free 29942, 29906, 29912 JE5556 Gpm3 Hfr F HfrC(leuS <- purE) lpm man JE5502 JE5503 lpm UidA+ JE5558 Ap2 F+ F man JE5500 JE5501 AroD+ JE5559 Gm6 Hfr F HfrC(leuS <- purE) man pps JE5502 JE5503 UidA+ JE5565 A'10 Hfr F HfrC(leuS <- purE) man pps JE5500 JE5504 AroD+ JE5583 p3 F+ F str gal aroD man JE5500 JE5501 Pps+ JE5586 102X2.1 rif F- galK2 trp man lpm pps str rif JE6082 JE5551 rif RifR JE5587 102X2.1/F'm-p- F+ F pps trp gal str lpm man JE5548 SPONTANEOUS Man- JE5591 GM291 Hfr F HfrP4X(lac <- proA) uidA1 G. Novel & M. Novel 29909 JE5596 G6 Hfr F HfrG6(metC <- argG) malB his Y. Hirota (Pasteur Institut) 30001, 35657 JE5602 353X42 F- tonA leu str arg? thr? ponB353 JE5600 JE5601 CROSS ponB353 PlS ProB+ Thy+ TR, AmpS JE5604 1104P7 F- trp? argH? thi? his? mtl? xyl? lac? malA str ponA1104 P4X8(JE5599) JE11104 CROSS ponA1104 PlS Thr+ Leu+ Thy+ X+ StrR MetB+ TR JE5607 LacM3 Hfr F HfrP4X8(lac <- proA) mtl xyl malA ponA980 P4x8(JE5599) JE10980 CROSS ponA980 Hfr Lac+ MetB+ P1S T5S TR JE5632 27'8 F- dacB12 dacA1191 aroB351 xyl str JE5605 SPONTANEOUS MeeillinamR TS on LA-NaCl JE5637 M4 Hfr F HfrC(leuS <- purE) tonA2 DE(lac)72 malA proA metB ponB85-21 85-21(JE10085) CD4(=JE6085) ponB85-21 MetD+ JE5640 L+1m F- ilvH611 tonA2 proA DE(lac)72 tsx dacA1191 dacB12 str ponA1104 ponB353 JE5623 JE5634 Leu+ TS in 1% NaCl L broth JE5646 1a#65 F- dacA1191 ponA1104 str DE(lac)72 dacB12 JE5603 JE5606 CROSS Xyl+ StrR ponA1104 : Ts JE5657 1atsmD4 F- dacA1191 ponA1104 dacB12 tsx? DE(lac72?) proA? metD1 metB str CD4(JE6085) JE5606 CROSS metD1 Xyl+ StrR L-Met 100_micro_g/ml supplemented JE5666 JE5603LV2 Hfr F HfrC(leuS <- purE) ilvH611 leuB ponB353 tonA DE(lac)72 malA proA metB MI183e(JE5774) JE5603 leu IlvH JE5668 1b.3ts#25 Hfr F HfrC(leuS <- purE) proA (malA)DE(lac)72 tsx ponB353 ftsI tonA metB JE5651 JE5666 ftsIts Leu+ ftsI : ts(MFT33) JE5673 528P1 F- his argH thi str malA ponA528 xyl mtl lacY trp P4X8(JE5599) JE10528 CROSS Pls X+ Thr+ Leu+ Thy+ StrR Met+ JE5674 568P18 F- ts568 ponA568 malA str thi argH his trp mtl xyl lacY P4X8(JE5599) JE10568 CROSS Pls Thr+ Leu+ Thy+ StrR Met+ JE5675 1022P3 F- str ponA1022 ts1022 malA thi argH his trp mtl xyl lacY P4X8(JE5599) JE11022 CROSS Pls Thr+ Leu+ Thy+ StrR Met+ JE5676 1099P8 F- mtl trp his argH thi str malA ponA1099 lacY xyl P4X8(JE5599) JE11099 CROSS Pls X+ Thr+ Leu+ Thy+ StrR Met+ JE5678 1262P3 F- str thi argH his trp mtl xyl lacY ponA1262 ts1262 malA P4X8(JE5599) JE11262 CROSS Pls X+ Leu+ Thy+ StrR Met+ Thr+ JE5680 A10 F- gal his nalA thy malA mtl dacB12 metC str lac JE5662 JE5712 CROSS dacB12 ArgG+ NalAR JE5689 1a-8 F+ F Other ftsI ponAts1104 ponB353 dacA1191 dacB12 tsx DE(lac72) proA tonA str JE5667 JE5699 CROSS ftsIts Leu+ MetB+ StrR ftsI : ts(MFT33) JE5692 LS9 F- str deoC thy JE5663 KL231(=JE5452) LeuS+ (TR) JE5697 JE5626/pLC29-47 F+ F Other dacA1191 dacB12 ponA980 malA xyl str JA200/pLC29-47 JE5626 CROSS pLC29-47 ColE1R StrR JE5699 JE5634/pLC29-47 F+ F Other dacA1191 ponB353 leu str tonA proA DE(lac) tsx ilvH ponAts1104 dacB12 JA200/pLC29-47 JE5634 CROSS pLC29-47 TR StrR JE5704 730rec/pLC26-6 F+ F Other thr argH his trp mtl xyl malA lacY str tonA recA1 leu thi ftsI730 JA200/pLC26-6 JE5717 CROSS pLC26-6 TR StrR JE5706 #21 F- str aroB metB tonA proA DE(lac) tsx dacB12 dacA1191 ponB353+ JE5667 JE5605 CROSS ponB353 Xyl+ StrR JE5719 1191-31 Hfr F HfrP4X8(lac <- proA) malA dacA1191 mtl xyl str his P4x8(JE5599) JE11191 CROSS Hfr X+ Lac+ MetB+ JE5728 3092 mal+ rif F- str lacY xyl thi mtl trp his thy argH thr leu rif tonA JE6082 PA3092 rif RifR Mal+ reverted JE5737 JE5666L+ 3ts ilvH Hfr F HfrC(leuS <- purE) ponB353 DE(lac) tsx metB proA tonA ilvH611 ftsI malA JE5659 JE5666 ftsItsMFT33 LeuB+ ftsI : ts(MFT33) JE5738 1ats 1b85.21 dacDL 22 F- metB DE(lac) tsx malA? ara? tonA? proA? lys ponAts1104 dacA1191 dacB12 ponB85.21 dap str JE5637 JE5649 CROSS ponB85.21 Leu+ StrR should be grown in L broth + NaCl1%+sncrose12%+MgSO4 0.2% 30C JE5745 21ov4/1b+#1 F+ F Other proA? trpE(Am)? tyr(am)? sup-126? str aroB dacA+? dacB12 rodAsp6 JA200/pLC19-19 JE5732 CROSS pLC19-19 ColE1RstrR CephaloridimeS JE5746 JE5732/1b+.1a- 3 F+ F Other dacA+? str proA? sup-126? trpE(Am)? tyr(am)? ponA980 rodAsp6 dacB12 JE5607 JE5745 ponA980 AroB+ ponA+ isogenic strain=JE5773 JE5747 21ov4LA2/3+#3 F+ F Other aroB ilvH leuA dacB12 rodAsp6 metB str JA200/pLC26-6 JE5734 CROSS pLC26-6 LeuA+ strR JE5748 21ov4LA2/1b+#2 F+ F Other leuA ilvH aroB str metB rodAsp6 dacB12 JA200/pLC19-19 JE5734 CROSS pLC19-19 ColE1R strR CephaloridimeS JE5749 JE5734/1b+ 1a-3 F+ F Other ilvH str metB rodAsp6 dacB12 leuA JE5607 JE5748 AroB+ ponA+ isogenic strain of JE5750 JE5752 Hfr F HfrC(leuS <- purE) DE(lac) metB tsx tonA leuA ponB353 proA malA JE6183 JE5726 leuA Thr+ JE5753 JE5752M+1 Hfr F HfrC(leuS <- purE) DE(lac) proA tonA leuA ponB353 tsx malA W3110 JE5752 metB+ MetB+ JE5754 1b-17 F+ F Other dacA1191 ponAts1104 ponB353 ftsI dacB12 tsx DE(lac)72 proA tonA str metB? JE5667 JE5700 CROSS ftsI : ts(MFT33) Leu+ StrR NaCl inhibits growth at 30C in L broth JE5755 #22 F- str aroB leuA dacB12 dacA1191 JE5752 JE5605 CROSS leuA Xyl+ StrR JE5758 JE5757/26-6#1 F+ F Other dacA1191 str DE(lac) tonA metB ponAts1104 ponB353 leuA dacB12 JA200/pLC26-6 JE5757 CROSS pLC26-6 LeuA+ StrR should be grown in 1% NaCl-L broth at 30C JE5767 PuG81 F- thi metA or B ile mtl xyl str lacY his trp tsx argG proC dacA1191 leu ara JE5664 LC102(=JE5597) CROSS dacA1191 PurE+ StrR JE5770 JA200/PLC19-19 F+ F Other dacA1191 ponA980 ftsI730 dacB12 str JA200/pLC19-19 JE5769 CROSS pLC19-19 ColE1R StrR JE5788 1191 td1 F- tonA dacA1191 X- thr leu thi argH thy his trp lacY mtl xyl malA str JE5593 JE11191 TR JE5828 his-3 F- his sup-126 rif thy tyr(am) trpE(Am) thr JE5528 JE5826 CROSS AroC+ MalB+ RifR JE5830 Trp-3 nalA1 Hfr F HfrG6(metC <- argG) lpm nalA lac malB JE5594 JE5807 nalA NalR (50_micro_g/ml) JE5839 A5/F'1334#1 F+ F his mtl galK lacY argE thi lpm tsx xyl EJ294/F'1334 JE5500 CROSS F'1334 His+ PyrD+ Thy+ Lpm- JE5846 K27 fadD+1 F- JE5500 JE5845 FadD+ JE5850 A314 Hfr F HfrP4X(lac <- proA) thi eda-2 metB N. Otsuji eda- = no growth on 0.2% glucuronic acid 29890 JE5851 A314 eda+2 Hfr F HfrP4X(lac <- proA) thi metB JE5500 JE5850 Eda+ JE5855 KLF48/KL159 F+ F gal thi-1 his-4 aroD5 proA2 recA1 xyl-5 or -7 tsx-1? or -29? supE44? lambda_- lac CGSC(B.Bachmann & M.Berlin) nalS 29912, 29942, 29906 JE5856 A5/KLF48#2 F+ F thi lpm galK lacY argE his tsx xyl mtl JE5855 JE5500 CROSS KLF48 His+ Pro+ Lpm- JE5868 MA27 Hfr F HfrC(leuS <- purE) lpo JE5514 JE5504 lpo Man+ AroD+ JE5874 M31 Hfr F HfrC(leuS <- purE) pps aroD lpo JE5514 JE5504 lpo Man+ JE5875 M3 Hfr F HfrC(leuS <- purE) lpo pps JE5514 JE5504 lpo Man+ JE5877 P4 Hfr F HfrC(leuS <- purE) aroD man JE5514 JE5504 Pps+ JE5882 P14 Hfr F HfrC(leuS <- purE) man lpo JE5514 JE5504 lpo Pps+ JE5893 A1 Hfr F HfrC(leuS <- purE) man JE5514 JE5504 AroD+ JE5897 A55/R68+ Hfr F HfrC(leuS <- purE) Other man pps lpo PAO1688 JE5513 CROSS R68 TCR Leu+ 29875, 29996, 30191 JE5898 A19/R68+ Hfr F HfrC(leuS <- purE) Other pps man PAO1688 JE5512 CROSS R68 TCR Leu+ 29996, 30191, 29875 JE5904 W3110 f1a++ F- IN(rrnD-rrnE)1 W3110 SPONTANEOUS swarm on NGA good swarmer JE5906 lpo-8 f1a++ F- gal lpo pps his proA argE thi lac xyl mtl tsx JE5505 SPONTANEOUS swarm on NGA good swarmer JE5907 lpo+4 gal+ f1a++ F- proA his pps tsx mtl xyl lac thi argE W3110 JE5506 gal+ Gal+ good swarmer JE5908 lpo-8 gal+ f1a++ F- lac mtl tsx lpo pps his proA argE thi xyl W3110 JE5505 gal+ Gal+ good swarmer JE5913 1360aroD+lpo-17 F- proA his man lpo thi lac gal tsx mtl xyl argE JE5510 JE5588 lpo AroD+ JE5916 lpo-2 F- Other Plkc str lpo argEH gal xyl lac mtl spc JE5517 JE5515 AroD+ JE5919 2 F- spc argEH gal xyl lac mtl str JE5508 JE5507 Man+ AroD+ JE5932 17 Hfr F HfrC(leuS <- purE) pps? JE5808 JE5504 Man+AroD+ JE5946 #4/F'lpo F+ F his argG str gal man aroD pps JE5555 JE5940 CROSS F3033 AroD+ Lac+ JE5947 5548AO F- pps gal str lpm man trp JE5548 CURING/AO Man- JE5950 102X2.1/F'lpo#10 F+ F pps man lpm trp gal str JE5555 JE5551 CROSS F3033 Pps+Arg+ JE5958 AO2 F- str lpm man pps trp gal JE5555 JE5551 CURING/AO Man- JE5971 G5 F- str pps lpm man trp rif thy JE5664 JE5585 CROSS Gal+RifR 35657 JE5972 TrG39 F- thy dacA1191 pps rif man lpm str JE5664 JE5585 CROSS dacA1191 Trp+RifR JE5973 TrG50 F- str man lpm pps thy rif JE5664 JE5585 CROSS Trp+RifR JE5974 Tr3 F- lpm rif str thy pps galK2 man JE5664 JE5585 CROSS Trp+RifR JE5976 MP44 F- thy str rif galK2 trp JE5664 JE5585 CROSS Man+RifR JE5978 ThH83 F- galK2 rif str his pps lpm man trp JE5664 JE5585 CROSS Thy+RifR JE5979 Th2 F- pps lpm man trp rif galK2 str JE5664 JE5585 CROSS Thy+RifR JE5991 64-3AB27 F- malA str his trp gal tsx lac tonA ara thi mtl JE5681 JE5434 CROSS IlvE+Thr+Leu+ JE6600 (CV530C att_lambda_)DE F- DE(lac-pro) leuA371 DE(gal-uvrB) thi CV530C DE(att_lambda_/_lambda_pleu i21nin5) SPONTANEOUS lambda_ phage curing from Yasuda JE6601 Hfr MFT33-1 Hfr F HfrP4X str leu ftsI33 (thi) P4x8 MFT33 CROSS ftsI33 on D-lac thr leu B1 ts:salt resque JE6606 PC1242=TKL-46 F- vtr his murF lacY xyl tonA tsx phx supE ths dra sus uvrB thr leu thi pyrF codA thyA argG ilvA PC 0417 E. J. J. Lugtenberg salt repairable 29899, 29892 JE6608 H-1119 F- purE murC E. J. J. Lugtenberg lysis/LB+-NaCl 41C-1h 29899, 29892 JE6610 ST222 F- thi trp azi strA ara mtl xyl gal lac murC thy his thr leu JE1011 salt repairable? 29900 JE6612 ST640 F- thi leu thr ara mtl str azi thy his trp lac mra gal xyl JE1011 salt repairable? (incomplete septation) 29900 JE6616 MRA 7201 F- mraST772 DE(pro-lac) thi ST772(=JE6615) JE6183 mra leu+ JE6623 MURE3721 F- DE(pro-lac) murE thy thi MURE3703=JE6605 OTHER METHODS (TMP) thy JE6625 MFT84 recA lacY1 malA1 xyl-7 mtl-2 mel tonA2 supE44 recA X- ftsB84 thr-1 leu-6 thi-1 argH1 his-1 trp-1 str-9 LC248 MFT84 thy CROSS recA Thy+ JE6627 MFT100 recA str-9 lacY1 malA1 xyl-7 mtl-2 mel tonA2 supE44 recA trp-1 his-1 argH1 thi-1 leu-6 thr-1 ftsB84 LC248 MFT100 thy CROSS recA? Thy+ MFT100 is UVSS, so that recA? JE6629 Hfr F HfrP4X polA1 (thi) mtl xyl P4X8 JE6268 CROSS A.A+ Lac+ JE6635 Hfr polA1-7 Hfr F HfrP4X xyl mtl (thi) polA1 P4X8 JE6268 CROSS polA1 a.a+ Lac+ JE6643 GT601 F- leu thr tonA mel mtl xyl malA lacY argH thyA trp his str supE thi Lin 6 MFT84 glpT TR JE6650 C600Sm thy#1 F- thr thi thy lacY tonA str supE leu C600Sm SPONTANEOUS thy TMP 862 JE6664 MA 135 F- proA-2 speB2 can-1 argE3 his trp thi lac str W. Maas, -> CGSC(B.Bachmann & M.Berlin) 29902, 29970 JE6669 AN180 F- str thi-1 argE3 AN119 JP58 ilu+ J. D. Butlin -> ? -> Auraku -> Takagaki -> isogenic strain to AN120 29877 JE6702 C600 pTSO 109 F- Other thr supE tonA lacY thi leu TF. AmpR DNA was given by M. Takanami pTSO 109 : Bam site5-HaeIII site2 of pSY221 + Bam HI* of pBR322 29753 JE6703 C600 pTSO 116 F- Other supE tonA lacY thi leu thr TF. AmpR DNA was given by M. Takanami pTSO116 : HaeIII site 2-3 of pSY221 + Bam HI* of pBR322 29753 JE6704 C600 pTSO 123 Other lacY tonA supE thr leu thi TF. AmpR DNA was given by M. Takanami pTSO 123 : HaeIII of pSY221 (contig ori) + BamHI* of pBR322 JE6705 C600 pTSO125 F- Other thi lacY tonA supE thr leu TF. AmpR DNA was given by M. Takanami pTSO125 : HaeIII of pSY221 contig ori + BamHI* of pBR322 29753 JE6706 C600 pTSO157 F- Other thi supE tonA lacY thr leu TF. AmpR DNA was given by M. Takanami pTSO157 : DE(BglII-BglII) of pTSO125 JE6707 C600 pTSO 164 F- Other thi leu thr lacY tonA supE TF. AmpR DNA was given by M. Takanami pTSO 164 : circularization of HindIII-A of pTSO 123 JE6708 C600 pTSO 177 F- Other leu thr thi lacY tonA supE TF. AmpR DNA was given by M. Takanami pTSO 177 : circularization of XhoI and SalI digest of pTSO125 JE6709 C600 pTSO 175 Other thr supE tonA lacY thi leu TF. AmpR DNA was prepaired from C600 pTSO175 obtained from Takanami JE6710 C600 pTSO 182 Other thr supE tonA lacY thi leu TF. AmpR DNA was prepared from C600 pTSO182 obtained from Takanami pTSO 182 : SalI-A of pTSO175+SalI-B(ori) of pTSO169 pTSO 169 : SalI-A* of pBR322 + HaeIII-D(ori) of pSY221 JE6711 C600 pTSO 190 Other supE thr leu thi lacY tonA C600 TF. DNA from Oka pTSO 190 : circularization of HindIII-A* of pTSO 182 JE6712 C600 pTSO 202 Other thr supE tonA lacY thi leu C600 TF. pTSO 202 : circularization of BamHI-A* of pTSO 196 JE6713 C600 pTSO 183 Other thi supE thr tonA lacY leu C600 TF. DNA from A. Oka pTSO 183 : same as pTSO 182 except orientation of ori JE6714 C600 pTSO 208 F- Other tonA thr leu thi lacY supE TF. DNA from A. Oka JE6715 C600 pTSO 209 F- Other leu thi lacY tonA supE thr TF. DNA from A. Oka JE6804 met16 F- str his tsx gal lac tonA ara thi mtl JE5662 JE5436 CROSS MetB+StrR JE6807 met34 F- argH lac gal his str malA mtl tsx thi ara tonA JE5662 JE5436 CROSS MetB+StrR JE6815 xyl12 F- mtl metB argH thi ara tonA lac tsx gal his str ilvA JE5662 JE5436 CROSS Xyl+StrR JE6823 TL39 F- lacY mtl xyl trp his thy malA str JE5662 PA3092 CROSS Thr+Leu+StrR JE6828 Hfr F HfrP4X8(lac <- proA) xyl JE5599 JE5716 CROSS MalA+Thy+MetB+ JE6834 TSX20 F- argH thr leu thi rif mtl his trp lacY JE5662 JE5728 CROSS Thy+RifR JE6835 TSX44 F- trp lacY thr dacB12 leu thi rif argH mtl his JE5662 JE5728 CROSS Thy+RifR JE6836 TX48 F- trp lacY thr leu thi rif argH mtl str his JE5662 JE5728 CROSS Thy+RifR JE6848 12BG21 F- dacB12 str JE5662 JE5775 argG+ AspB+ JE6849 12GB21 F- str dacB12 JE5662 JE5775 aspB+ ArgG+ JE6875 980rev1 F- mtl xyl malA ponA980 thi his thy str tonA thr argH leu ponBS980 trp lacY JE10980 SPONTANEOUS TR JE6877 TLS70 F- ponA980 ponB980 tonA thi argH thy his trp lacY mtl xyl malA str JE5599 JE10980 CROSS Thr+ Leu+ StrR JE6878 TLS1 F- trp his thy ponA980 str malA xyl mtl lacY JE5599 JE10980 CROSS Thr+ Leu+ StrR JE6886 G1 Hfr F HfrP4X8(lac <- proA) xyl thi? met? ile? str argG mtl JE5607 LC102thy(=JE6888) CROSS Gal+ StrR 35657 JE6887 G2 F- thi? met? purE? xyl mtl ara leu lac his str ile? tsx? proC? JE5607 LC102thy(=JE6888) CROSS Gal+ StrR 35657 JE6898 980X8 Hfr F HfrP4X8(lac <- proA) thr ponA980 ponB980 ts980 dacA1191 leu thi argH thy his malA str JE6866 JE10980 CROSS Xyl+Dap+Trp+ JE6901 980X13 thy F- dacB64 thy thr his leu thi argH str lacY malA ponA980 ponB980 ts980 JE6897 SPONTANEOUS thy TrimethoprimR JE6904 980XT2 td3 Hfr F HfrP4X8?(lac <- proA) dacB64 str malA his thi? leu thr dacA1191 ponB980 ponA980 W3110 JE6896 TR JE6915 TS9 F- str rif gal lpm? pps thy JE5607 JE5585 CROSS Trp+StrR JE6935 151-72 F- malA str JE5672 JE5435 AroB+ JE6936 151-14 F- str ponA151 JE5672 JE5435 AroB+ JE6937 151-15 F- str JE5672 JE5435 AroB+ JE6944 568-3 F- str ponA568 malA JE5674 JE5435 ponA568 AroB+ JE6950 1022-24 F- ponA1022 str JE5675 JE5435 ponA1022 AroB+ JE6951 1099-32 F- str ponA1099 malA JE5676 JE5435 ponA1099 AroB+ JE6952 1099-33 F- str malA JE5676 JE5435 AroB+ JE6954 1099-35 F- ponA1099 str JE5676 JE5435 ponA1099 AroB+ JE6960 1262-54 F- str ponA1262 JE5678 JE5435 ponA1262 AroB+ JE6964 1104-3 F- xyl str malA dacB12 dacA1191 JE5604 JE5605 AroB+ ponA+ isogenic strain of JE5351 JE6967 MS7 Hfr F HfrC(leuS <- purE) metB DE(lac72) tsx malA JE5620 JE6085 MetD+ JE6979 704Hfr-11 Hfr F HfrP4X8(lac <- proA) ts704 thr leu thi argH thy his trp mtl xyl malA str tonA ponB704 JE5599 JE10704 CROSS ponB704 & Hfr Lac+StrRMetB+ Plkc poor growth JE6980 704 Hfr-14 Hfr F HfrP4X8(lac <- proA) argH his mtl xyl malA str ts704 thi JE5599 JE10704 CROSS Hfr Lac+StrRMetB+ PlS JE6982 704 Hfr-17 Hfr F HfrP4X8(lac <- proA) ts704 thr leu thi argH his tonA str mtl xyl malA JE5599 JE10704 CROSS Hfr Lac+StrRMetB+ Plkc poor growth JE6988 704XT26 Hfr F HfrP4X8(lac <- proA) dacB64 his ts str dacA1191 malA JE6866 JE10704 CROSS dacA1191 dacB64 Xyl+Thy+Trp+Dap+ JE6992 M23 Hfr F HfrC(leuS <- purE) malA metB DE(lac72) JE10085 JE6085 MetD+ JE6993 D22 F- str purE trp his argG ile metA or B thi leu xyl gal mtl ara JE10085 JE5481 DapD+ ts on L - Nacl + 1%-Na.citrate
tr on L - Nacl JE7000 353X6 F- ponB353 thr? leu arg? str tonA ts353 JE5600 JE5601 CROSS ponB353 & Pls ProB+Thy+ TS , Amps JE7001 1238lip3 F- str galK xyl mtl purB thi rodA1238 supE44 lacY proA his JE5679 JE5453 rodA1238 Lip+ ts on LA+1%Na.Citrate-NaCl tr on LA-NaCl ovoid at 42C in L-NaCl rod at 30C in L-NaCl JE7011 151 male25 Hfr F HfrP4X8(lac <- proA) argH trp thy? his JE5599 JE10151 CROSS Hfr Lac+MetB+ MecillinamR, TR JE7016 1116 lip1 F- xyl thi his proA purB mtl galK lacY str supE44 JE7015 JE5453 Lip+ Slow growth JE7019 lip FLR1 F- galK lacY str supE44 purB proA his thi lip-9 xyl mtl JE5453 SPONTANEOUS MecillinamR Slow growth TS? on LA+1% Citrate-Na-NaCl JE7021 27'12 F- aroB xyl str dacB12 dacA1191 JE5679 JE5605 MecillinamR(20_micro_g/ml) TS on LA+1%Na-Citrate - NaCl JE7044 730rev17-1 F- thi ftsI730 thr leu argH thy his trp lacY mtl xyl malA str tonA JE10730 SPONTANEOUS TR PenGR at 42C PBC3- JE7046 730/pLC 26-6 F+ F Other xyl malA str tonA ftsI730 thr leu thi argH thy his trp lacY mtl JA200/pLC26-6 JE10730 CROSS pLC26-6 TR StrR JE7050 #6 _lambda_+ F- λ+ tsx proC lacY trp gal his ftsI730 dacA1191 dacB12 leu metA or B thi ile mtl xyl str JE5631 OTHER METHODS lambda_ infection lambda_+ lambda_R JE7051 730/pLC4-14 F- Other trp ftsI730 thr leu thi argH thy his lacY mtl xyl malA str tonA JA200/pLC4-14 JE10730 CROSS pLC4-14 TR StrR JE7061 M3.1' F- lacY thr thi metA or B ile mtl trp gal tsx proC dacA1191 dacB12 or 64 ponB353 ts353 str tonA his JE5629 JE5625 CROSS Xyl+Thy+ JE7066 dacB.1b85-21-22 F-? dacB12? tsx DE(lac) proA tonA metB str malA ponB85-21 JE5637 JE6843 CROSS ponB 85-21 Mtl+StrR JE7072 1b#80 F- dacB12 tonA metB malA str ponB353 dacA1191 JE5603 JE5606 CROSS ponB353 Xyl+StrR JE7075 1a#1 F- dacA1191 ponAts1104 str proA DE(lac) JE5603 JE5606 CROSS Xyl+StrR JE7077 1a#3 F- str metB ponAts1104 dacA1191 dacB12 JE5603 JE5606 CROSS Xyl+StrR JE7078 1a+1b+#81 F- str dacB12 dacA1191 DE(lac) malA JE5603 JE5606 CROSS Xyl+StrR JE7079 1a+1b+#5 F- dacA1191 str malA metB dacB12 JE5603 JE5606 CROSS Xyl+StrR JE7080 1a+1b+#21 F- malA ponAts1104 dacA1191 dacB12 metB str JE5603 JE5606 CROSS Xyl+StrR JE7081 1a+1b+#38 F- malA dacB12 dacA1191 ponAts1104 str JE5603 JE5606 CROSS Xyl+StrR JE7082 1a1b#18 spc F- str tonA metB spc ponAts1104 ponB353 dacA1191 dacB12 JE6082 JE7070 Spc SpcR lysed at 42C should be grown in 1% NaCl-L broth at 30C JE7085 1b argG mal 29 F- gal tonA ponB353 leu malA argG purE ara thi metA or B ile mtl his trp JE5603 JE5609 CROSS malA Xyl+ProA+ JE7087 m7 Hfr F HfrC(leuS <- purE) ponB85-21 tonA metB proA DE(lac) tsx JE5606 JE5637 Mal+ 30C JE7088 dac 8-2 F- str proA DE(lac) tsx dacA1191? ponB85-21 tonA metB? JE5637 JE5606 CROSS Xyl+StrR 30C ts (salt repairable) JE7089 dac8-3 F- metB? proA? DE(lac?) ponAts1104? dacA1191? str tonA tsx? dacB12 ponB85-21 JE5637 JE5606 CROSS Xyl+StrR 30C ts (salt repairable) JE7097 528-0 F- tsx ponB353 dacA1191 dacB12 str tonA malA JE5673 JE5610 AroB+ JE7098 528-6 F- dacA1191 ponB353 dacB12 str tonA tsx JE5673 JE5610 AroB+ JE7303 5636 pB1 aroB+#3 F- str tonA rodAts? dacB12 dacA1191 ponB353 ponAts1104 metB? proA? DE(lac?) tsx? JE5606 JE7100 ponAts1104 AroB+ JE7305 7303 ilvH 3-6 F- tonA str ilvH tsx? DE(lac?) proA? metB? ponAts1104 ponB353 dacA1191 dacB12 rodAts? JE5659 JE7303 IlvH JE7307 1a-14-6 F+ F Other dacB12 dacA1191 ftsI tonA proA DE(lac) ponAts1104 tsx str ponB353 nalA recAl JE5594 JE7306 CROSS recAl NalRStrR ftsI : ts(MFT33) JE7308 F- DE(lac) ponB353 leuA dacB12 dacA1191 str tonA metB JE5751 JE5756 AroB+ cf. ponAts1104 isogenic strain = JE5757 JE7310 911male90 Hfr F HfrP4X8(lac <- proA) xyl ts-911 thy mtl P4x8 (=JE5599) JE10911 CROSS Hfr ts-911 X+Lac+MetB+ JE7312 911male95 Hfr F HfrP4X8(lac <- proA) man malA xyl mtl str trp his thy ts-911 JE5599 JE10911 CROSS Hfr ts-911 X+Lac+MetB+ JE7316 JE57601b+dapD9 F- dapD str DE(lac) metB ftsI730 dacA1191 dacB12 aroB JE5481 JE5760 dapD ponB+ JE7330 Hfr thyA his galK tonA leu thr tgt malA str lacY P13 JE10907 CROSS xyl+StrR JE7331 Hfr lacY thr leu tonA thyA str tgt P13 JE10907 CROSS xyl+StrR JE7332 F- metA or B tgt ileS ara tsx purE galK trp his argG xyl mtl JE7331 LC102 CROSS ProC+ThyA+ JE7340 F- thi str tonA xyl mtl malA lacY supE argH thyA his trp leu thr W3110 JE10911 TR JE7343 ? not tested metA or B mtl xyl argG his trp galK ara ileS tgt JE7331 LC102 CROSS ProC+ThyA+ JE7350 ? not tested lacY entA trp str xyl mtl thi tgt leu ara tonA JE7330 AN193 CROSS ProC+His+ThyA+ JE7405 lip+AB#9 F- lacY1 thi-1 his-4 proA2 purB15 mtl-1 xyl-5 galK2 str-35 supE44 JE7403 JE5453 Lip+ TR. PlS cf. PBP-5-isogenic = JE7401&JE7402 JE7406 SP6trAB#1 F- ponA ilv trpE9829(Am) sup-126 tyr(am) JE7403 JE5780(=SP6) RodA+ (TR) cf. PBP-5-isogenic = JE7408 & JE7409 JE7408 SP6 trAB#3 F- trpE9829(Am) ilv tyr(am) pfv ponA sup-126 JE7403 JE5780 pfv RodA+ (TR) cf. PBP-5+isogenic = JE7406 & JE7407 JE7409 SP6trAB#4 F- tyr(am) ilv sup-126 ponA pfv trpE9829(Am) JE7403 JE5780 pfv RodA+ (TR) cf. PBP-5+isogenic = JE7406 & JE7407 JE7458 555 P1S Hfr F HfrP4X8?(lac <- proA) thi xyl str ara metA or B ile malA gal purE his trp tonA dacB555 P1S leu mtl JE5721 JE10555 CROSS P1S X+Thr+ArgH+Thy+Lac+ArgG+ JE7459 1S-12-11 F- dacB12 dacA1191 str xyl JE5662 JE7455 dacB12 ArgG+ JE7460 4+12-2 F- str xyl dacB12 JE5662 JE7456 dacB12 ArgG+ JE7461 1S12-10 F- dacA1191 xyl str JE5662 JE7455 ArgG+ JE7462 4+12-12 F- str xyl JE5662 JE7456 ArgG+ JE7463 1S64-11 F- xyl dacA1191 str JE7457 JE7455 ArgG+ JE7468 1S555-8 F- xyl dacA1191 str JE7458 JE7455 ArgG+ JE7481 P4x8-pfv-32 Hfr F HfrP4X8(lac <- proA) argE pfv str galK mtl thi? JE5599 JE7403 CROSS Hfr Lac+MetB+ JE7497 LS16 F- pfv thy str deoC JE7403 JE5452 pfv LeuS+ JE7499 lS18 F- deoC str thy JE7403 JE5452 LeuS+ JE7501 38 F- str lacY galK proA mtl his thi dacA1191 xyl purB supE JE5663 JE5453 dacA1191 Lip+ JE7507 5 F- purB thi his proA mtl xyl galK lacY str supE JE5693 JE5453 Lip+ JE7511 27 F- str deoC thy JE5693 JE5452 LeuS+ JE7513 7 F- thi leu proC trpE supE44 mtl xyl tsx tonA azi str ara lacY dacA1191 JE5693 JE7493 dacA1191 EntA+ JE7515 9 F- trpE leu thi tsx ara lacY str azi tonA xyl mtl supE44 proC JE5693 JE7493 EntA+ JE7517 Lip+leuS+1 F- galK thi his proA purB mtl xyl lacY str supE JE5724 JE5453 Lip+ JE7523 LeuS+ent-7 F- deoC str thy entA JE7493 JE5452 entA LeuS+ JE7525 RodA+ent-1 F- sup-126 ponA entA tyr(am) trp(am) ilv JE7493 JE5780 entA RodA+ JE7526 RodA+ent+5 F- ilv sup-126 trp(am) tyr(am) ponA JE7493 JE5780 RodA+ JE7527 Lip+ent+1 F- mtl xyl galK lacY str supE thi his proA purB JE7493 JE5453 Lip+ JE7529 Lip+ent-7 F- supE str lacY galK xyl thi mtl purB proA his entA JE7493 JE5453 entA Lip+ JE7531 EntA+lip+2 F- str thi proC leu ara lacY trpE xyl mtl supE44 tsx tonA azi JE5453 JE7493 EntA+ JE7533 EntA+lip-3 F- thi lip-9 lacY ara str azi tonA tsx xyl mtl supE44 trpE proC leu JE5453 JE7493 lip-9 EntA+ JE7536 4 F- thi pbp-S1704 lac his str arg JE6983 JE7535 CROSS AroC+MalA+ JE7540 xEM11 F- pbp-S1704 lac str arg thi JE6983 JE7535 CROSS AroC+MalA+ JE7568 50 F- gnd eda str nalA JE7564 JE7565 CROSS PtsF+ArgH+ JE7582 td1 Hfr F HfrP4X8(lac <- proA) thi thr ilvH leu JE5774 JE7476 Sep+ JE7603 MI183eL+33#2 F- str lacZ xyl lys gal ftsI tsx trp proC purE metE thi tonA JE5651 JE5774 ftsI Leu+ ftsI (MFT33) JE7610 7598rec3 F- thi metE xyl str recA1 JE5595 JE7598 CROSS recA1 Lys+Trp+PurE+StrR JE7611 7598rec5 F- thi metE proC xyl lacZ recA1 str tonA tsx ftsI730 JE5595 JE7598 CROSS recA1 Lys+Trp+PurE+StrR JE7612 7602rec2 F- lacZ recA1 ftsI tsx tonA str xyl proC metE thi JE5595 JE7602 CROSS recA1 Lys+Trp+PurE+StrR ftsI (MFT33) JE7613 7609rec3 F- lacZ thi metE proC xyl recA1 sep2158 tsx tonA str JE5595 JE7609 CROSS recA1 Lys+Trp+PurE+StrR JE7615 R32recA F- trp recA1 thr leu thi? argH lacY mtl xyl malA str ts JE5595 JE7614 CROSS recA1 Thy+His+StrR at 30C poor growth, top-10+? JE7619 LM1 Hfr F HfrP4X8(lac <- proA) leu tonA top-250? trp str JE5599 JE7626 CROSS Lac+Met+ JE7620 LM28 Hfr F HfrP4X8(lac <- proA) thy his trp top-250? mtl xyl JE5599 JE7626 CROSS Lac+Met+ JE7621 LM47 Hfr F HfrP4X8(lac <- proA) his trp argH thr leu tonA top-250? mtl xyl malA JE5599 JE7626 CROSS Lac+Met+ JE7624 LS7 Hfr F HfrP4X8(lac <- proA) leu tonA top-250? str thr argH mtl xyl malA JE5599 JE7626 CROSS Lac+StrR JE7629 AN10 F- tonA? thi argH leu xyl str mtl nalA his galK lacY thr? glc? metK? metG? metB? JE7630 JE7561 CROSS Ara+NalR JE7630 Hfr#19 Hfr F HfrP4X8(lac <- proA) ts str xyl metB argH thi? leu tonA dda-1040 JE5599 JE11040 CROSS Hfr Lac+StrR N. Hirasawa JE7631 Hfr#3 Hfr F HfrP4X8(lac <- proA) tonA ts dda-1040+ trp his str xyl mtl argH thi? leu JE5599 JE11040 CROSS Hfr Lac+StrR JE7632 MN9 F- argH dda-1040 proA leu ara thi xyl str nalA his galK lacY tonA? thr? metB? glc? metK? metG? JE7630 JE7561 CROSS dda-1040 Mtl+NalR JE7634 MA8 F- metK? tonA? ts lacY glc? galK his str xyl mtl leu thi thr? JE7631 JE7561 CROSS MetG+ArgH+ JE7639 GN2 ? not tested thi argH mtl xyl str nalA metG his tonA? thr? metK? glc? leu JE7631 JE7561 CROSS GalK+NalR ME5313 Hfr PC3-P4X Hfr F Hfr(proB -> Lac) his thi dnaG3 str purE ME5309 (HfrABCE#21) PC34 CROSS dnaG3 Thy+Leu+Lac+ ME5316 FF8011 F- str proC ptsH T. Nagata 29972 ME5319 FF8020 F- ptsH proC str T. Nagata 29972 ME5323 Hfr T46-P13 Hfr F HfrP13(pyrE-dnaA) his thi arg thr leu lac mal gal ara dnaA46 P13 CRT4624 CROSS Hfr Xyl+Cys+ ME5324 AT2092,CGSC3579 F- lacY1 or Z4 malA1 xyl-7 mtl-2 strA8,-9 or -14 tonA2 or -14 tsx-23 or -25 supE44? lambda_R (lambda)-? argH1 hisG1 pheA2::Mu purF1 thi-1 M. Abe,CGSC(B.Bachmann & M.Berlin) gly-? : mini colony without Gly 29991, 30008 ME5362 AT2457 F- glyA X- M. Abe ME5426 PC1075,AT3143 F- his proC pyrF pdxC purE ilv met lac xyl tonA tsx str Phabagen Collection ME5449 WM301 F- arg thyA deo gal his lac leu met pro str trp hsdK12 A. TAKETO ME5455 AN379 Hfr F pnrE nagB str F. Gibson ME5464 AT3141 Hfr F HfrG6(metC <- argG) Other Mu:in proA proA30 thi-1 supE44 leu-6 argD37 thr-1 str-8 lacZ4 CGSC(B.Bachmann & M.Berlin) 29941 ME5470 DF71,CGSC4880 Hfr F HfrH eda-1 rel-1 thi-1 (lambda)- lacI22 Y. KOMEDA,CGSC(B.Bachmann & M.Berlin) 29992 ME5483 PK8 F- Other ColV(xyl -> malA) colVR thr leu thi lac azi colE1R C600 P. Kahn 29946 ME5492 HfrBT313 Hfr F HfrP4X str thy dnaB313 P4x8 CR34BT313 CROSS Ts Lac+strR Thr+Leu+ Y. NISHIMURA ME6101 CRT46 spc recA#7 F- mal lac leu ilv dnaAT46 str spc thr rec LC248 CRT46 spc CROSS recA- thy+str ME7267 C600(PBR322) F- Other supE44 thr-1 leu-6 thi-1 lacY1 tonA21 C600 TF. ApR A. Nishimura ME8078 CSR603,CGSC5830 F- tsx-33 argE3 thi-1 uvrA6 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL31 supE44 thr-1 leuB6 proA2 phr-1 recA1 C. S. Rupert,CGSC(B.Bachmann & M.Berlin) mucous colony ME8310 IQ402 F- zfe-99::Tn5 supE44? (lambda)- rpsL mtl-2 xyl-7 lacY1 or lacZ4 thi-1 relA1? argH1 IQ99 JK84 TD./Others GlyA+ KmR 30097 ME8359 CBK090#1 F- thyA (lambda)-DE(lacA-argF)? pyrB::Tn5 W3110 thyA 29759, 30087 ME8360 CBK239#1 F- (lambda)-DE(lacA-argF) thyA argI::Tn5 W3110 thyA 29759, 30087 ME8376 NK6073#1 F- pro endL purF::Tn10 hsdR N. Kleckner require 10_micro_g/ml Ade in MM 29853 ME8382 NFB203 F- lac rpsL putA purE pyrC46 zcd-202::Tn10 gltA his Co-td with putA : 25% 30085 ME8431 NK6051,CGSC6186 Hfr F HfrH(valS <- uxuAB) DE(gpt-lac)5 purE79::Tn10 relA1 spoT1 thi-1 (lambda)- T. Nagata,CGSC(B.Bachmann & M.Berlin) 29821 ME9030 LCB498#1,CGSC4445 F- rpsL9 malT1(lambda_R) glgA1 xyl-7 mtlA2 grgH1 thi-1 hisG1 rfbD1 galP63 purE43 lacY1 pro-33 fhuA2 leuB6 thr-1 supE44 gal-6 (lambda)- CGSC(B.Bachmann & M.Berlin) 29994, 29954 ME9031 LCB499#1,CGSC4446 F- mtl-2 rpsL9 tonA2 (lambda)- supE44 pro-33 leuB6 thr-1 purE43 hisG1 argH1 thi-1 lacY1 gal-6 malA1(lambda_R) glgB2 xyl-7 CGSC(B.Bachmann & M.Berlin) CGSC4446 29994, 29954 ME9032 LCB618#1,CGSC4447 F- galP63 rpsL9 gltB31 malT1 (lambda_R) glgC3 xyl-7 mtl-2 argH1 thi-1 thr-1 leuB6 fhuA2 pro-33 lacY1 purE43 supE44 gal-6 (lambda)- cur-1 hisG1 rfbD1 CGSC(B.Bachmann & M.Berlin) CGSC4447 29994, 29954 ME9033 DH5 F- supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Takara Shuzo Co., Ltd 30081, 84955, 35386, 35355, 3313 ME9035 Q358,RDB004 hsdR phi_80R supE RIKEN DNA BANK host for lambda_phage(su+)Vector ME9036 ED8767,RDB018 supF58 supE44 galK2 recA56 hsdS3(r-B m-B) metB1 galT22 RIKEN DNA BANK host for cosmid vector ME9037 HMS174,RDB026 rifR hsdR recA1 RIKEN DNA BANK host for initial cloning into pET vectors and maintaining 29852 ME9038 DH5_alpha ,RDB108 hsdR17 endA1 gyrA96 thi-1 relA1 supE44 DE(lacU169(phi_80 (lacZ)DE(M15))) recA1 RIKEN DNA BANK RDB108, host for plasmid vector ME9039 BB4,RDB131 F+ F galK2 tonA metB1 trpR55 HsdR514 supE44 supF58 galT22 U169 DE(lac) RIKEN DNA BANK RDB131,host for plasmid vector ME9040 XL1-BLUE F+ F gyrA46 supE44 hsdR17 recA1 endA1 thi relA1 lac TOYOBO Co.LTD 3270 ME9041 JM110 F+ F dam dcm supE44 hsdR17 thi leu rpsL lacY galK galT ara tonA thr tsx DE(lac-proAB) TOYOBO Co.LTD 29828 ME9042 BL21(DE3) λ+ (lambda_cIts857 ind1 Sam7 nin5 lacUV5-T7 gene1) gal hsdS TOYOBO Co.LTD 3187, 3188, 35664, 3960, 3942, 3314, 3299, 3210, 3205, 3204, 3193, 3192 ME9043 JM101 supE DE(lac-proAB) thi RIKEN DNA BANK host for pUC plasmid Vector ME9044 MG1655 F- 3953 ME9045 TK3D-01,CGSC6447 F- trkD1 rha-4 DE(kdpABC-gltA)219 trkA405 thi-1 DE(galK-bioD)76 CGSC(B.Bachmann & M.Berlin) Require 120mM-KCl DE(galK-bioD)76 results in very poor growth 29751 ME9046 TK2204 F- kdpA4 lacZ(am) rha thi trkD1 trkA405 nagA W. Epstein Require 120mM-KCl ME9047 TK2469 F- λ+ lambda_p1<209> kdpA24::lacZYA thi rha DE(lac-argI)179 nagA trkA405 trkD1 W. Epstein Require 120mM-KCl 29791 ME9048 TK2691 F- trkH1 trkD1 trkG92 thi DE(kdpFAB)5 nadA lacZ rha W. Epstein Require 120mM-KCl ME9049 WP2/pKM101 Other trp umuCD JAPAN BIO ASSAY RESEARCH CENTER pKM101 : Ap20_micro_g/ml 29766, 30194 ME9050 WP2uvrA/pKM101 Other uvrA trp umuCD JAPAN BIO ASSAY RESEARCH CENTER UVS pKM101 : Ap20_micro_g/ml 29766, 30194 ME9051 CH1692 DE(Tn10) rpsL supD74(ts) argA topA57(Am) DE(tolC) DE(lac-514) ME8780 SPONTANEOUS ME8748 CBK103 F- thy cysG::Tn5 DE(lacA-argF)? (lambda)- 30087 ME9100 AQ151 F- rpsL31 argE3 his-4 proA2 galK2 xyl-5 mtl-1 ara-14 thr-1 thi-1 leuB6 lacY1 sup-37 tsx-33 from D'Ari Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JM1: Casterallazzi et al., M. G. G. 1972; 119, 153-174. 29762 ME9101 AQ152 F- proA2 thr-1 his-4 argE3 rpsL31 tsx-33 sup-37 tif-1 leuB6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 from D'Ari Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JM12: Casterallazzi et al., M. G. G. 1972; 119, 153-174.
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.

29762 ME9102 AQ153 F- leuB6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL31 tsx-33 sup-37 tif-1 lexA4 thr-1 from D'Ari Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JM124: Casterallazzi et al., M. G. G. 1972; 119, 153-174.
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 29762 ME9103 AQ157 F- leuB6 his-4 rpsL31 tsx-33 sup-37 lexA3 argE3 proA2 mtl-1 xyl-5 ara-14 galK2 lacY1 thi-1 thr-1 from D.W.Mount Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as DM49: Mount D. W., 1977, Proc. Natl. Acad. sci. USA, 74, 300-304.
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9104 AQ159 F- rpsL31 thr-1 leuB6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 tsx-33 sup-37 lexA3 tif-1 sfiA11 from David Mount Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as DM1180: Mount D. W., 1977, Proc. Natl. Acad. sci. USA, 74, 300-304.
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 29762 ME9105 AQ160 F- lexA3 sfiA11 spr-51( lexA51 ) xyl-5 mtl-1 proA2 his-4 argE3 rpsL31 tsx-33 sup-37 tif-1 thr-1 leuB6 thi-1 lacY1 galK2 ara-14 DM1180 MUTAGENESIS/Others Mitomycin-C reisistant MitomycinC resistant from David Mount Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as DM1187: Mount D. W., 1977, Proc. Natl. Acad. sci. USA, 74, 300-304.
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 29762 ME9107 AQ169 Hfr F HfrKL16(po61) HfrKL16 recB21 sbcA8 thr-300 ilv-318 spc300 Recommended medium:
Luria broth supplemented with glucose (0.1%). same as JC5412
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
ME9109 AQ229 Hfr F HfrH HfrH Δ((pro-lac) thi from CSH collection Recommended medium:
Luria broth supplemented with glucose (0.1%). same as CSH63: Miller J. H., 1972, Experimental genetics. Cold spring harbor laboratory, Cold spring harbor, N. Y.
30102 ME9111 AQ271 F- nalA18 proB48 his-29 metE90 trpA9605 trpR55 lacI22 lacZ118 rpsL171 azi-9 from B. Bachmann Recommended medium:
M9 salts glucose medium supplemented with Casamino acids (0.2%), Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as KL320: Lloyd R. G. and Low B. 1976, Genetics, 84, 675-695. 30102 ME9112 AQ290 F- F thi Δ((pro-lac) rpsL sup+(su-) ara thyA HfrH(CSH63) AQ284 (as CBO129) CROSS Pro-, Δ((pro-lac) Leu+, Str-r Recommended medium:
Luria broth supplemented with glucose (0.1%). 30102 ME9113 AQ291 F- nalA18 thyA708 deo-29 proB48 his-29 metE90 trpA9605 trpR55 azi-9 rpsL171 lacZ118 lacI22 AQ271 OTHER METHODS MUTAGENESIS/Others Trimethoprim selection Thy-, thyA708 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30102 ME9114 AQ300 Hfr F HfrC thyA42 HfrC metE101 arg-19 rnhA102 deoB20 HfrC(SL230) AQ145 CROSS HfrC Trp+, Pyr+ Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). E. coli15T-
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30102 ME9115 AQ331 F- rnhA102 rpsL thi thyA AQ300(HfrC) AQ290 CROSS cSDR, rnhA102 Pro+, Arg+ Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30102 ME9117 AQ377 F- nalA18 azi-9 rpsL171 lacZ118 recA200 trpR55 trpA9605 metE90 lacI22 rnhA102 deo-29 thyA708 KL268 AQ345 CROSS UV(Ts), recA200 His+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. 30102 ME9118 AQ527 F- rnhA102 metE90 trpA9605 trpR55 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 recA200 rin-15 KL268 AQ377 MUTAGENESIS/Others CROSS EMS treatment rin-15 SDR(stable DNA replication) at 42C by autoradiography Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. 30102 ME9120 AQ536 Hfr F HfrKL16 recA56 HfrKL16 rpsE300 thr-300 ilv-318 srlC300::Tn10 from Clark Recommended medium:
Luria broth supplemented with glucose (0.1%). same as JC10240: Csonska L. N. and Clark A. J. 1980, J. Bacteriol., 143, 529-530.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
29843 ME9121 AQ542 Hfr F HfrRa-2(PO77) sfiA11 HfrRa-2(PO77) lexA3 mal-28 Recommended medium:
Luria broth supplemented with glucose (0.1%). same as DM972
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. ME9122 AQ543 F- rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA56 srlC300::Tn10 metE90 trpA9605 trpR55 lacI22 lacZ118 JC10240 AQ377 TD./PI Rec-(30C), recA56 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
30102 ME9123 AQ547 F- azi-9 nalA18 thyA708 deo-29 rnhA102 recA56 srlC300::Tn10 trpA9605 metE90 trpR55 lacI22 lacZ118 rpsL171 rin-15 JC10240 AQ527 TD./PI UV-s, recA56 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
30102 ME9124 AQ548 F- rpsL lac thiA metB1 thi thy dnaE293(Ts) SL51 AQ466 CROSS Ts, dnaE293(Ts) Leu+, Met- from Connaughton Recommended medium:
Luria broth supplemented with glucose (0.1%). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30131 ME9126 AQ559 F- lexB30 thi-1 thr-1 leu-6 pyrF43 thyA6 deoC1 lacY1 tonA21 supE44 from D'Ari Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as GY1163: Morand et al., 1977,J. Bacteriol., 131, 572-582.
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30102 ME9127 AQ569 F- pyrF43 leu-6 thr-1 thi-1 lexB30 srlC::Tn10 supE44 tonA21 lacY1 deoC1 thyA6 AQ536(JC10240) AQ559(GY1163) TD./PI srlC300::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30102 ME9128 AQ575 F- thyA thi rpsL rnhA102 srlC300::Tn10 JC10240 AQ331 TD./PI srlC300::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30102 ME9129 AQ580 Hfr F HfrRa-2 Sfa-4 lexA3 argE::Tn10 mal-28 Recommended medium:
Luria broth supplemented with glucose (0.1%), tetracycline (20μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 29810 ME9130 AQ598 F- srlC300::Tn10 lexB30 rnhA102 thi thy AQ569 AQ331 TD./PI UV-s, lexB30 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30102 ME9131 AQ624 F- rpoB340 ilv proB (or proC) his-29 deoB (or deoC) metB1 trpA9605 thyA argH AQ614(LS534) SPONTANEOUS thyA low thyimine requirement Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30168 ME9132 AQ634 F- proB trpA9605 his-29 ilv metB1 thyA deoB (or deoC) PC2 AQ624 TD./PI Rif-s, rpoB+ Arg+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30065, 3211 ME9133 AQ659 F- rpsL polA1 thy lacY14 rha malB from J. Wechsler Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JW164
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. 29843 ME9136 AQ666 F- deoB (or deoC) thyA metB1 rnhA224 ilv pro his-29 trpA9605 Ls Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30065 ME9137 AQ677 F- his-29 rnhA224 deoB (or deoC) thyA metB1 ilv trpA9605 SL230 (HfrC) AQ666 CROSS pro+ Pro+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9138 AQ685 F- thyA deoB (or deoC) rpoB340 lac-3 metD88 trpA9605 his-29 metB1 argH proA3 CD4(HfrC) AQ683 CROSS ilv+ Ilv+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30168 ME9139 AQ694 F- proA3 trpA9605 his-29 metB1 argH thyA lac-3 rpoB340 deoB (or deoC) rnhA224 AQ677 AQ685 TD./PI cSDR+, rnhA224 MetD+ Recommended medium:
M9 salts glucose medium supplemented with Casamino acids (0.2%), Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30168 ME9141 AQ705 F- deoB/C thyA pro trpA9605 his-29 dnaA46 metB1 AQ401(EH116) AQ634 TD./PI Ts, dnaA46 Ilv+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30113 ME9142 AQ738 F- metB1 rnhA102 metD88 rpoB340 deoB (or C) thyA trpA9605 his-29 Δlac-3 AQ300 AQ685 TD./PI cSDR, rnhA102 Pro+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29843 ME9143 AQ742 F- lacZ118 metE90 trpA9605 trpR55 lacI22 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 ΔrecA306 srlR301::Tn10 rin-15 JC10284 AQ527 TD./PI UVs, recA306-del Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. 30102 ME9144 AQ751 Hfr F Hfr (serA-<-lysA) thi-1 argA21 drm-3 recA200 srlC300::Tn10 Hfr (serA-<-lysA) AQ749 AQ270 TD./PI UV(Ts), recA200 Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. 30134 ME9146 AQ845 Hfr F HfrC malA36 tsx76 HfrC proA3 zag::Tn10 metD88 relA1 lac-3 metB1 from D. Clark Recommended medium:
Luria broth supplemented with glucose (0.1%). same as DC407 29843 ME9149 AQ978 F- zag::Tn10 his-29 pro ilv metB1 thyA deoB (or deoC) trpA9605 rnhA224 AQ845(DC407) AQ666 TD./PI zag::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29843 ME9150 AQ979 F- trpA9605 thyA metB1 ilv pro his-29 zag::Tn10 deoB (or deoC) AQ845 AQ666 Lr, rnh+ Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Lr: resistance for rich media.
Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
29843 ME9156 AQ1065 F- Δlac-3 metD88 proA3 deoB (or deoC) rpoB340 thyA argH metB1 his-29 trpA9605 dnaA5 ilv-192 740 AQ1034 TD./PI Ts, dnaA5 Suc+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as DK65 30168 ME9157 AQ1130 F- Δlac asnA31 asnB50::Tn5 ara thi gyrA from A. Wright Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as R2D2: Felton etal., 1980, J. Bacteriol., 142, 221-228. 30168 ME9158 AQ1173 F- deoB (or deoC) rpoB340 asnB50::Tn5 thyA argH metB1 trpA9605 his-29 dnaA5 tnaA600::Tn10 ilv rnhA224 Δlac-3 proA3 R2D2 AQ1043 TD./PI asnB50::Tn5 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). same as DK173
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30168 ME9166 AQ1355 F- dasF373 thyA deoB/C asnB::Tn5 argH metD88 rpoB340 ilv-192 trpA9605 his-29 metB1 dnaA5 TC373 DK175 TD./PI Ls, dasF373 Pro+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). same as DK175
Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30065 ME9167 AQ1363 F- his-29 trpA9605 ilv-192 rpoB340 metD88 argH asnB::Tn5 deoB/C thyA dasF377 dnaA5 metB1 TC377 DK175 Ls, dasF377 Pro+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30065 ME9168 AQ1409 F- lacY14(am) thyA deoC2 lacZ rpsL zig::Tn10 polA1 Rha+, Met+ Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). same as CM5280: Kelly W. S., 1980, Genetics, 95, 15-38.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. 30144 ME9170 AQ1427 F- Other rnhA224 zag::Tn10 polA1 thyA AQ978 AQ1347 TD./PI rnhA224 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml). This strain is Lr. Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29843 ME9174 AQ1553 F- Δlac-3 rnhA224 Δ(srlR-recA)306 srlR::Tn10 trpA9605 his-29 metB1 argH thyA rpoB340 deoB (or deoC) metD88 AQ633 AQ699 TD./PI UV-s, Δ(srlR-recA)306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29843 ME9265 AQ3456 F- recAo254 deo-29 srlC300::Tn10 lexA3 lacI22 trpR55 trpA9605 metE90 lacZ118 rpsL171 azi-9 nalA18 thyA708 rin-15 rnhA102 recA200 AQ2984 AQ3404 TD./PI UV-r, recAo254 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). AQ3404 is same as AQ3403
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29810 ME9266 AQ3474 F- lacI22 metE90 trpA9605 trpR55 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 sfiA11 AQ3394 SPONTANEOUS Km-s, pyrD+ Ura+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 29810 ME9267 AQ3476 F- lacI22 lacZ118 rpsL171 azi-9 nalA18 metE90 trpA9605 trpR55 lexA71::Tn5 sfiA11 recA200 rnhA102 deo-29 thyA708 AQ2153 AQ3474 TD./PI lexA71::Tn5 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 29810 ME9273 AQ3580 F- his-29 trpA9605 pro deoB (or deoC) tnaA::Tn10 dnaA5 thyA metB1 ilv AQ634 AQ3408 TD./PI Ts, dnaA5 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Ts 3305, 30142 ME9274 AQ3588 F+ F' relA1 supE44 recA1 Δ(lac-pro) thi gyrA96 hsdR17 endA1 Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JM109: Yanisch-Perron et al., 1985, Gene, 33, 103-119.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30130 ME9275 AQ3589 F- nalA18 metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 thyA708 deo-29 rnhA102 recA200 rin-15 tnaA::Tn10 dnaA5 AQ1103 AQ527 TD./PI Ls, dnaA5 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29810 ME9418 AQ8190 F- λ+ λp[sfiA::lac cI(Ind-)] rnhA224 λp[sfiA::lac cI(Ind-)] rpsL Δ(pro-lac) pro zag::Tn10 AQ8017 AQ978 TD./PI Ls, rnhA224 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9419 AQ8194 F- recJ284::dTn10(Tc-s) deoB(or deoC) thyA metB1 ilv pro his-29 trpA9605 recG258::miniTn10Km AQ8034 AQ8022 TD./PI UVs, recG258 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30132 ME9420 AQ8198 F- λ+ λp[sfiA::lac cI(Ind-)] Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] ruvC51 eda-51::Tn10 pro AQ7946 AQ8107 TD./PI UVs, ruvC51 Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30123 ME9593 AQ10479 F- tsx-33 argE3 his-4 proA2 galK2 mtl-1 xyl-5 ara-14 priA2::kan sfiA11 thyA sup-37 lacY1 thi-1 leu-6 thr-1 rpsL-31 AQ10071 AQ10459 TD./PI filamentous, priA2 Km-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30139 ME9595 AQ10576 F- ilv trpA9605 his-29 pro metB1 thyA deoB (or deoC) lexA3 malF3089::Tn10 AQ8300 AQ634 TD./PI UVs, lexA3 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30142 ME9596 AQ10601 F- trpA9605 his-29 pro ilv metB1 malF3089::Tn10 lexA3 proC::Tn5 Δ(lacIPOZY)x74 deoB (or deoC) thyA AQ8300 AQ9504 TD./PI UVs, lexA3 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30142 ME9176 AQ1613 F- deoB (or deoC) Δlac-3 rnhA224 recA56 srlC300::Tn10 his-29 metB1 argH thyA rpoB340 metD88 trpA9605 AQ536 AQ699 TD./PI UV-s, recA56 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29843 ME9177 AQ1629 F- endA supE thi- rna metE proA Recommended medium:
Luria broth supplemented with glucose (0.1%). same as ON112: Ogawa T. and Okazaki T., 1983, M. G. G. 30065 ME9178 AQ1630 F- rnh-91 proA metE endA rna thi- supE Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as ON152: Ogawa T. and Okazaki T., 1983, M. G. G.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30065 ME9179 AQ1631 F- rnh-59 thia- supE proA metE endA rna Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as ON121: Ogawa T. and Okazaki T., 1983, M. G. G.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30065 ME9183 AQ1732 F- his-29 metB1 argH thyA deoB (or deoC) rpoB340 proA3 rnh-91 dnaA5 asnB::Tn5 Δlac-3 trpA9605 ON152 AQ1175 TD./PI Ls, rnh-91 Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30065 ME9186 AQ1786 F- argH proA3 metD88 Δlac-3 srlR::Tn10 Δ(srlR-recA)306 rpoB340 thyA deoB (or deoC) metB1 his-29 trpA9605 JC10284(AQ663) AQ685 TD./PI UV-s, Δ(srlR-recA)306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. 29843 ME9193 AQ1874 F- rpsL151 rha-5 lacZ53 deoC2 thyA36 metE70 Recommended medium:
Luria broth supplemented with glucose (0.1%). same as JG108 ME9194 AQ1883 F- thyA rin-15 metD88 trpA9605 rnhA102 recA200 rpoB340 argH deo AQ600 AQ745 TD./PI rin-15 Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. ME9195 AQ1994 F- metD88 trpA9605 his-29 argH rnhA224 thyA deoB/C TC743 AQ699 TD./PI Rif-s, rpoB+ Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM3440
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29835 ME9196 AQ1995 F- dnaA46 tna::Tn10 deoB/C thyA rnhA224 metD88 his-29 trpA9605 TC743 AQ1081 TD./PI Rif-s, rpoB+ Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). same as CM3442
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29835 ME9197 AQ1997 F- metD88 dnaA850::Tn10 deoB/C thyA rnhA224 his-29 trpA9605 TC743 AQ1249 TD./PI Rif-s, rpoB+ Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). same as CM3452
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29844 ME9198 AQ2016 F- proA3 thyA asnB50::Tn5 metD88 deoB/C ilv-192 his-29 trpA9605 dnaA5 AQ1993 AQ1175 TD./PI Rif-s, rpoB+ Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). 29835 ME9199 AQ2020 F- thyA asnB50::Tn5 metD88 argH proA3 his-29 trpA9605 ilv-192 dnaA5 rnhA224 deoB/C TC743 AQ1173 TD./PI Rif-s, rpoB+ Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29835 ME9202 AQ2096 F- thyA deoB (or C) trpA9605 his-29 argH dnaA850::Tn10 rnhA224 Δlac-3 metD88 Rif-s, rpoB+ Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29835 ME9203 AQ2106 F- thr-1 sfiA11 tif-1 dinD1::Mu d(lac Ap) leu-6 his-4 argE3 ilv(ts) galK2 str-31 lac Δ(U169) Recommended medium:
Luria broth supplemented with glucose (0.1%). same as GW1040: Kenyon C. J. and Walker G. C., 1980, Proc. natl Acad. Sci. USA, 77, 2819-2823. 29820 ME9204 AQ2118 F- trpA9605 dnaA850::Tn10 rnhA224 Δlac-3 metD88 recA200 deoB (or deoC) thyA argH AQ270 AQ2096 TD./PI Ts, recA200 His+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29835 ME9206 AQ2149 F- recF332::Tn3 from A. J. Clark. Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JC10990
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30132 ME9209 AQ2152 F- lexA3(Ind-) xyl-5 mtl-1 proA2 his-4 argE3 rpsL31 tsx-33 supE44 recA281o(Con) srlC300::Tn10 thr-1 leuB6 thi-1 lacY1 galK2 ara-14 Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JC11867: Clark A. J., 1982, Biochimie, 64, 669-675.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29799 ME9210 AQ2153 F- thr-1 lexA71::Tn5 srlC300::Tn10 rpsL31 galK2 ilv(ts) argE3 his-4 proA2 leuB6 from A. J. Clark Recommended medium:
Luria broth supplemented with glucose (0.1%). same as K197
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 29810 ME9211 AQ2178 F- metB1 trpA9605 his-29 proB(or proC) ilv thyA deoB(or deoC) zig::Tn10 polA1 CM5280 AQ634 TD./PI MMS-s, polA1 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. 30114 ME9216 AQ2200 F- recB21 sbcC15 recF143 bglR lacY1 galK2 ara-14 xyl-5 mtl-1 rpsL31 tsx-33 supE4 recC22 thr-1 leuB6 proA2 his-4 argE3 thi-1 from A. J. Clark. Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JC7539
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29810 ME9218 AQ2288 F- trpA9605 his-29 proB(or proC) ilv metB1 thyA tnaA::Tn10 deoB(or deoC) recF322::Tn3 AQ2149(JC10990) AQ634 TD./PI UVs, recF322::Tn3 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29843 ME9219 AQ2294 F- recF322::Tn3 trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) rnhA224 tnaA::Tn10 AQ2149 AQ666 TD./PI UVs, recF322::Tn3 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
29843 ME9221 AQ2369 F- recA200 dnaA850::Tn10 metE90 trpA9605 trpR55 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 AQ1249 AQ377 TD./PI Ts, dnaA850::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29810 ME9223 AQ2441 F- rpsL171 recF322::Tn3 recA200 rnhA102 deo-29 thyA708 nalA18 azi-9 rin-15 lacZ118 lacI22 trpR55 trpA9605 metE90 AQ2149(JC10990) AQ527 TD./PI UVs, recF322::Tn10 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
29810 ME9224 AQ2452 F- metB1 ilv pro his-29 trpA9605 recF332::Tn3 rnhA224 deoB (or deoC) thyA AQ2149(JC10990) AQ666 TD./PI UVs, recF322::Tn3 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
29843 ME9297 AQ3821 F- supE44 zif-90::pML31 dnaA46 his-4 metE46 trp-3 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) AQ3746 AQ3804 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9298 AQ3823 F- ton-1 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 rpsL8(or 9) supE44 zif-90::pML31 dnaA205 AQ3746 AQ3805 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9299 AQ3825 F- rpsL8(or 9) ton-1 tsx-3 dnaA204 zif-90::pML31 supE44 ara-9 mtl-1 lacY1(or lacZ4) galK2 thi-1 his-4 trp-3 metE46 AQ3746 AQ3806 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9300 AQ3827 F- ara-9 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 tsx-3 ton-1 rpsL8(or 9) zif-90::pML31 supE44 dnaA203 AQ3746 AQ3807 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9301 AQ3829 F- tsx-3 ton-1 rpsL8(or 9) ara-9 mtl-1 lacY1(or lacZ4) galK2 thi-1 his-4 trp-3 metE46 dnaA508 zif-90::pML31 supE44 AQ3746 AQ3808 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9302 AQ3831 F- trp-3 metE46 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 zif-90::pML31 dnaA167 AQ3746 AQ3809 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9303 AQ3833 F- supE44 zif-90::pML31 dnaA602 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) AQ3746 AQ3810 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9304 AQ3835 F- supE44 zif-90::pML31 dnaA601 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) AQ3746 AQ3811 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9305 AQ3837 F- tsx-3 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 ton-1 rpsL8(or 9) supE44 zif-90::pML31 dnaA604 AQ3746 AQ3812 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9306 AQ3839 F- zif-90::pML31 dnaA606 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9tsx-3 ton-1 rpsL8(or 9) supE44 AQ3746 AQ3813 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9309 AQ4191 F- galK2 oriCdel-1071 supE44 rpsL8 (or 9) ton-1 tsx-3 ara-9 mtl-1 lacY1(or lacZ4) zif-90::pML31 thi-1 his-4 trp-3 metE46 AQ3788 AQ3817 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9310 AQ4221 F- htpR6(Am) rpsL thi Δlac araD zhf-50::Tn10 Recommended medium:
Luria broth supplemented with glucose (0.1%), tetracycline (20μg/ml). same as KY1429: Tobe et al., 1984, Mol. Gen. Genet., 195, 10-16. 29813 ME9312 AQ4249 F- rpsL8 (or 9) supE44 zif-90::pML31 oriCdel-1071 dnaA5 lacY1(or lacZ4) galK2 thi-1 trp-3 his-4 metE46 mtl-1 ara-9 tsx-3 ton-1 AQ3788 AQ3821 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9313 AQ4251 F- ara-9 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 tsx-3 ton-1 rpsL8 (or 9) supE44 zif-90::pML31 oriCdel-1071 dnaA46 AQ3788 AQ3821 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9314 AQ4253 F- ara-9 mtl-1 lacY1(or lacZ4) galK2 thi-1 his-4 trp-3 metE46 dnaA205 oriCdel-1071 zif-90::pML31 supE44 rpsL8(or 9) ton-1 tsx-3 AQ3788 AQ3823 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9315 AQ4255 F- mtl-1 ara-9 tsx-3 ton-1 galK2 his-4 trp-3 metE46 thi-1 lacY1(or lacZ4) dnaA204 oriCdel-1071 zif-90::pML31 rpsL8 (or 9) supE44 AQ3788 AQ3825 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9316 AQ4257 F- lacY1(or lacZ4) supE44 zif-90::pML31 oriCdel-1071 dnaA203 his-4 thi-1 galK2 rpsL8(or 9) mtl-1 ara-9 tsx-3 ton-1 trp-3 metE46 AQ3788 AQ3827 oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9317 AQ4259 F- trp-3 metE46 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 zif-90::pML31 oriCdel-1071 dnaA508 AQ3788 AQ3829 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9318 AQ4262 F- rpsL8(or 9) supE44 zif-90::pML31 oriCdel-1071 dnaA167 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 AQ3788 AQ3831 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9319 AQ4263 F- zif-90::pML31 oriCdel-1071 dnaA602 supE44 rpsL8(or 9) metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 AQ3788 AQ3833 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9320 AQ4265 F- ton-1 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 rpsL8(or 9) supE44 zif-90::pML31 oriCdel-1071 dnaA601 AQ3788 AQ3835 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9225 AQ2454 F- zig::Tn10 deoB (or deoC) thyA metB1 ilv pro trpA9605 his-29 recF332::Tn3 polA12 AQ2149(JC10990) AQ1523 TD./PI UVs, recF322::Tn3 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. 29843 ME9226 AQ2456 F- ilv metB1 thyA deoB (or deoC) rnhA224 zig::Tn10 polA12 recF332::Tn3 trpA9605 his-29 pro AQ2149(JC10990) AQ1525 TD./PI UVs, recF322::Tn3 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29843 ME9227 AQ2547 F- pro deoB (or deoC) thyA his-29 trpA9605 metB dnaA5 ilv AQ1993 AQ1065 TD./PI Rif-s, rpoB+ Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30144 ME9228 AQ2561 Hfr F HFrH deoC thi- galE Δ(att-bio) deoA102 lysA cytR upp udp zga::Tn10 fuc relA1 AQ2458 AQ2550 TD./PI Arg+, argA+ Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). 29844 ME9229 AQ2584 F- Other metB1 thyA trpA9605 deoB (or deoC) his-29 pro ilv recA200 polA1 rnhA224 AQ265 AQ2199 CROSS UV(Ts), recA200 His+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). transformed with pBR322
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29843 ME9230 AQ2591 F- trpA9605 argH recF143 ilv Δlac-3 metD88 deoB (or deoC) rpoB340 thyA metB1 his-29 AQ2200 AQ1065 TD./PI UVs, recF143 Tr(42C) Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. 29794 ME9233 AQ2652 F- deoC2 rha-5 recA200 srlC::Tn10 thyA36 rpsL151 lacZ53 AQ1883 AQ2072 TD./PI Rin+, rin+ Met+, metE+ Recommended medium:
M9 salts glucose medium supplemented with Casamino acids (0.2%), Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29810 ME9235 AQ2696 F- his-29 trpA9605 argH thyA deoB (or deoC) proA3 metD88 AQ1993 AQ685 TD./PI Rif-s, rpoB+ Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29835 ME9236 AQ2781 F- trpA9605 his-29 proB ilv metB1 deoB (or deoC) AQ165(JC5519) AQ634 TD./PI UVr Thy+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29799 ME9237 AQ2782 F- trpA9605 his-29 proB ilv metB1 recB21 recC22 deoB (or deoC) AQ165 AQ634 TD./PI UVs, recB21 recC22 Thy+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29799 ME9238 AQ2787 F- trpA9605 proB ilv metB1 thyA recA200 deoB (or deoC) rnhA224 AQ270 AQ666 CROSS UV(Ts); recA200, Thy-; thyA His+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.

30127 ME9240 AQ2873 F- argE::Tn10 lexA3 rin-15 trpA9605 trpR55 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 metE90 AQ580 AQ527 MUTAGENESIS/Others TD./PI UVs, lexA3 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29810 ME9241 AQ2891 F- deoC2 lacZ53 rpsL151 rin-15 zag::Tn10 rnhA224 rha-5 recA200 srlC::Tn10 thyA6 AQ978 AQ2657 TD./PI Ls, cSDR, rnhA224 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29810 ME9242 AQ2894 F- srlC::Tn10 deoC2 lacZ53 rpsL151 zag::Tn10 rnhA224 rha-5 recA200 thyA6 AQ978 AQ2652 TD./PI Ls, cSDR+, rnhA224 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29810 ME9243 AQ2923 F- azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 rin-15 bglR recF143 trpR55 trpA9605 metE90 lacI22 lacZ118 rpsL171 AQ2200 AQ527 TD./PI UVs, recF143 Bgl(arubutin)+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29810 ME9244 AQ2984 F- rpsL31 recA254o recA200 mtl-1 xyl-5 galK2 ara-14 lacZ118 lacI3 argE3 supE44 srlC300::Tn10 proB48 his-4 thi-1 tsx-33 Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). same as N1715: Lloyd R. G., 1983, M. G. G., 189, 157-161.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29810 ME9245 AQ2987 F- recN261 tyrA16::Tn10 Recommended medium:
Luria broth supplemented with glucose (0.1%). same as SP215: Picksley et al., 1984, M. G. G., 195, 267-274.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9247 AQ3021 F- xyl-5 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA recF144 thr-1 leu-6 thi-1 lacY1 galK2 ara-14 mtl-1 from A. J. Clark. Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JC8931
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29810 ME9248 AQ3022 F- mtl-1 thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA ΔrecF349 dnaA508 tnaA300::Tn10 from A. J. Clark. Recommended medium:
Luria broth supplemented with glucose (0.1%). same as JC14065
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29810 ME9249 AQ3023 F- thi-1 lacY1 galK2 tsx-33 rpsL-31 argE3 his-4 proA2 mtl-1 xyl-5 thyA sup-37 recJ284::Tn10 thr-1 ara-14 leu-6 Recommended medium:
Luria broth supplemented with glucose (0.1%). same as JC12123: Lovet S. T. and Clark A. J., 1984, J. Bacteriol., 157, 190-196.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9251 AQ3033 F- metE90 rin-15 tnaA::Tn10 rnhA102 recA200 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 trpR55 ΔrecF349 dnaA508 deo-29 trpA9605 AQ3022 AQ527 TD./PI Ts, UVs, ΔrecF349 Tc-r(30C) Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29810 ME9252 AQ3036 F- trpR55 thyA708 nalA18 azi-9 deo-29 rnhA102 recA200 rin-15 tnaA::Tn10 lacZ118 rpsL171 dnaA508 lacI22 metE90 trpA9605 AQ3022 AQ527 TD./PI Tr, UVr, recF+ Tc-r(30C) Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29810 ME9254 AQ3154 F- lacI22 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 rin-15 tnaA::Tn10 dnaA5 recF144 metE90 trpA9605 trpR55 lacZ118 AQ3123 AQ527 TD./PI UV-s(30C), Ts, recF144 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29810 ME9255 AQ3200 F- dX lacZ8305::Mu cts62/Mu Recommended medium:
Luria broth supplemented with glucose (0.1%). same as CAG5050: Baker et al., 1983, J. Bacteriol., 156, 970-974. 29813 ME9259 AQ3362 F- trpA9605 his-29 pro ilv thyA metB1 deoB (or deoC) recJ284::Tn10 AQ3032 AQ634 TD./PI UV-s, recJ284::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9260 AQ3364 F- metB1 dnaA5 tnaA::Tn10 metD88 proA3 deoB (or deoC) rpoB340 Δlac-3 thyA argH his-29 trpA9605 TC745 AQ685 TD./PI Ts, dnaA5 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30130 ME9262 AQ3403 F- thyA708 metE90 trpA9605 azi-9 rpsL171 trpR55 lacZ118 lacI22 lexA3 recA200 rnhA102 deo-29 nalA18 AQ2871 SPONTANEOUS Tc-s, arg+ Arg+ Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: : Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 29810 ME9264 AQ3408 F- dnaA5 thyA Δlac-3 deoB (or deoC) proA3 argH his-29 trpA9605 metD88 tnaA::Tn10 AQ799 AQ3364 Rif-s, rpoB+ Met+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30142 ME9276 AQ3625 F- sbcA23 thr-1 leuB6 thi-1 lacY1 galK2 ara-4 xyl-5 mtl-1 proA2 his-60 argE3 rpsL31 tsx-33 supE44 recB21 recC22 from A. J. Clark. Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JC8679
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30132, 59194, 56351, 56352 ME9278 AQ3626 F- thr-1 mtl-1 xyl-5 ara-4 galK2 lacY1 thi-1 leuB6 recE159 sbcA23 recC22 recB21 supE44 tsx-33 rpsL31 argE3 his-60 proA2 from A. J. Clark. Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as JC8691
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30132 ME9279 AQ3634 F- proA2 thr-1 his-4 argE3 rpsL31 recB270(Ts) thi Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as SK119: Kushner S. R., 1974, J. Bacteriol., 136, 1213-1218.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29799 ME9280 AQ3746 F- deoB (or deoC) trpA9605 his-29 argH thyA proA3 metD88 Δlac3 ilv dnaA5 zif-90::pML31 AQ3742 SPONTANEOUS zif-90::pML31 Tr Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29811 ME9281 AQ3764 F- rnhA339::cat deoB (or C) thyA metB1 ilv proB (or proC) his-29 trpA9605 AQ3297 AQ634 TD./PI rnhA339::cat Cm-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30113 ME9283 AQ3802 F- galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 metE46 trp-3 his-4 thi-1 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM735: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9284 AQ3803 F- rpsL8(or 9) mtl-1 dnaA5 supE44 galK2 thi-1 his-4 trp-3 metE46 ara-9 tsx-3 lacY1(or lacZ4) ton-1 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM740: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9285 AQ3804 F- rpsL8 (or 9) metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 supE44 dnaA46 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM742: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9286 AQ3805 F- dnaA205 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8 (or 9) supE44 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM744: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9287 AQ3806 F- dnaA204 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM746: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9288 AQ3807 F- dnaA203 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM748: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9289 AQ3808 F- dnaA508 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM2555: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9291 AQ3810 F- dnaA602 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM2733: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9292 AQ3811 F- ara-9 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 tsx-3 ton-1 rpsL8(or 9) supE44 dnaA601 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM2735: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9293 AQ3812 F- metE46 dnaA604 supE44 rpsL8(or 9) ton-1 tsx-3 ara-9 mtl-1 lacY1(or lacZ4) galK2 thi-1 his-4 trp-3 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM2738: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9294 AQ3813 F- dnaA606 supE44 rpsL8(or 9) ton-1 tsx-3 ara-9 mtl-1 lacY1(or lacZ4) galK2 thi-1 his-4 trp-3 metE46 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as CM2740: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396. 29811 ME9295 AQ3817 F- mtl-1 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 zif-90::pML31 AQ3746 AQ3802 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9296 AQ3819 F- zif-90::pML31 supE44 trp-3 metE46 galK2 his-4 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 thi-1 dnaA5 rpsL8(or 9) AQ3746 AQ3803 TD./PI zif-90::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9321 AQ4267 F- galK2 supE44 dnaA604 oriCdel-1071 zif-90::pML31 metE46 trp-3 his-4 thi-1 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) AQ3788 AQ3837 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9322 AQ4269 F- mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) metE46 trp-3 his-4 thi-1 supE44 zif-90::pML31 oriCdel-1071 dnaA606 galK2 lacY1(or lacZ4) AQ3788 AQ3839 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29811 ME9325 AQ4319 F- thi-1 thr-1 leu-6 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA nfo-1::kan relA1 spoT thiA Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as BW540: Cunningham et al., 1986, J. Bacteriol., 168, 1120-1127. 29813 ME9326 AQ4335 F- rpsL galK galU (lac)del-x74 (ara leu)del-7697 = (araABIOC)del araD139 Recommended medium:
Luria broth supplemented with glucose (0.1%). same as MC1000 ME9327 AQ4382 F- ton-1 rpsL8(or rpsL9) supE44 thyA deoB(or deoC) zif::pML31 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 metE46 trp-3 his-4 AQ3746 AQ4241 TD./PI zif::pML31 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29789 ME9328 AQ4401 F- zif::pML31 oriCdel-1071 lacY1(or lacZ4) mtl-1 ara-9 galK2 tsx-3 rpsL8(or 9) ton-1 thi-1 his-4 trp-3 metE46 supE44 thyA deoB(or C) AQ3788 AQ4382 TD./PI oriCdel-1071 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29789 ME9330 AQ4432 F- zga::Tn10 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 thyA deoB(or C) recB270 AQ4406 AQ4241 TD./PI UVs(Ts), recB270 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30134 ME9333 AQ4517 F- Δlac-4169 sup(am) soi-17::lacZ rpsL AQ4063 MUTAGENESIS/Others MudX phage LacZ inducible with paraquat, soi-17::lacZ Ap-r Recommended medium:
Luria broth supplemented with glucose (0.1%). 29813 ME9334 AQ4519 F- Δlac-4169 sup(am) soi-19::lacZ rpsL AQ4063 MUTAGENESIS/Others MudX phage LacZ inducible with paraquat, soi-19::lacZ Ap-r Recommended medium:
Luria broth supplemented with glucose (0.1%). 29813 ME9336 AQ4548 F- katG::Tn10 Recommended medium:
Luria broth supplemented with glucose (0.1%). same as UM202: Loewen P. C., 1983, J. Bacteriol., 159, 418-420. 29813 ME9337 AQ4591 F- Δ(argF-lac)205 araD139 flbB5301 non-9 gyrA219 relA1 metE70 btuB460::Tn10 ΔoxyR3 argE::Tn10 AQ896 AQ4547 TD./PI H2O2-s, ΔoxyR3 Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). 29813 ME9340 AQ4647 F- thyA ilv proB(or proC) his-29 trpA9605 deoB(or deoC) argE::Tn10 ΔoxyR3 metB1 AQ4591 AQ634 TD./PI H2O2-s, ΔoxyR3 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29812 ME9342 AQ4708 F- soi-28::lacZ rpsL (lac)del-4169 sup(am) htpR6(Am) zhf50::Tn10 AQ4221 AQ4258 TD./PI Ts, htpR6(am) Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). 29813 ME9343 AQ4718 F- ΔoxyR3 rpsL Δ(lac)4169 sup(am) soi-28::lacZ AQ4591 AQ4523 TD./PI H2O2-s, ΔoxyR3 Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). 29813 ME9344 AQ4742 F- Mu cts-62 sup(am) Δ(lac)U169 rpsL AQ4063(GC4468) OTHER METHODS lysogenized Recommended medium:
Luria broth supplemented with glucose (0.1%). AQ4063 lysogenized with Mu cts-62 29813 ME9347 AQ4800 F- recF322::Tn3 thyA deoB (or deoC) rnhA224 ilv metB1 pro his-29 trpA9605 AQ2149(JC10990) AQ666 TD./PI recF322::Tn3 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30127 ME9348 AQ5004 F- sup-37 thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 thyA from C. Davern Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30122 ME9354 AQ5441 F- tsx-3 ton-1 rpsL8(or 9) supE44 thyA deoB(or C) zga::dTn10(Tc-s) rnhA224 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 AQ5423 OTHER METHODS Bochner Selection zga::dTn10(Tc-s) Tc-s Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 29789 ME9355 AQ5486 F- tsx-3 tnaA600::Tn10 rpsL8(or 9) supE44 dnaA46 ton-1 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 AQ5425 AQ3804 TD./PI Ts, dnaA46 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30116 ME9356 AQ5496 F- supE44 metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) thyA deoB(or C) zga::dTn10(Tc-s) rnhA224 dnaA850::Tn10 AQ3519 AQ5441 TD./PI dnaA850::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044. 29789 ME9359 AQ5786 F- thyA metB1 ilv proB(or C) trpA9605 his-29 zga::Tn10 deoB(or C) AQ2561 AQ634 TD./PI Thy-, thyA Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 29799 ME9431 AQ8368 F- λ+ λp[sfiA::lac cI(Ind-)] ruvC51 λp[sfiA::lac cI(Ind-)] rpsL Δ(pro-lac) pro recG258::miniTn10kan eda-51::Tn10 AQ8034 AQ8198 TD./PI UVs, recG258 Km-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30123 ME9432 AQ8384 F- λ+ λp[sfiA::lac cI(Ind-)] Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] rnhA224 recB21 zga::Tn10 pro AQ8045 AQ8246 TD./PI UVs, recB21 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Ts, Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30134 ME9433 AQ8386 F- λ+ λp[sfiA::lac cI(Ind-)] λp[sfiA::lac cI(Ind-)] zig::Tn10 rpsL Δ(pro-lac) pro polA12 rnhA339::cat AQ1524 AQ8136 TD./PI UVs, polA12 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9434 AQ8387 F- λ+ λp[sfiA::lac cI(Ind-)] polA12 rpsL Δ(pro-lac) pro zig::Tn10 λp[sfiA::lac cI(Ind-)] AQ1524 AQ8107 TD./PI UVs, polA12 Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9435 AQ8426 F- trpA9605 his-29 pro ilv metB1 thyA deoB(or deoC) polA12 zig::Tn10 AQ1524 AQ634 UVs, polA12 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9436 AQ8428 F- trpA9605 polA12 rnhA339::cat deoB (or deoC) thyA metB1 ilv pro his-29 zig::Tn10 AQ1524 AQ8034 TD./PI UVs, polA12 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30134 ME9360 AQ5931 F- zga::Tn10 deoB(or deoC) metB1 ilv proB(or proC) his-29 trpA9605 recB21 AQ169 AQ5786 TD./PI UV-s, Rec-, recB21 Thy+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29799 ME9361 AQ5937 F- Δ(gpt-proA)62 galK2 thr-1 leuB6 lac+ ilv(ts) sulA221 lexA71::Tn5 recA441 ara-14 lac xyl-5 mtl-1 tsx-33 supE44 zga::Tn10 recB21 rpsL31 thi-1 argE3 his-4 AQ5931 AQ2125(JM441) TD./PI UV-s, Rec-, recB21 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29799 ME9362 AQ6039 F- rha-5 lacZ53 rpsL151 thyA36 polA12 deoC2 IN(rrnD-rrnE)1 Recommended medium:
Luria broth supplemented with glucose (0.1%). 30118 ME9363 AQ6152 F- Other his-4 srlC300::Tn10 lexA3(Ind-) recA281(Con) supE44 tsx-33 rpsL31 argE3 thr-1 leuB6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 JC11867 TF. Ap-r, Cm-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29799 ME9364 AQ6226 F- Other srlC300::Tn10 proA2 leuB6 thi-1 lacY1 galK2 ara-14 xyl-5 rpsL31 argE3 his-4 thr-1 mtl-1 lexA3(Ind-) recA281(Con) supE44 tsx-33 JC11867 TF. Ap-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
29799 ME9375 AQ7539 F- supF6(Ts) thyA deo thr ilv metE trpE9829(Am) tyrA(am) rnhA199(am) Recommended medium:
Luria broth supplemented with glucose (0.1%). same as YT378: Murakami et al., 1987, J. Bacteriol., 169, 1724-1730.
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30116 ME9376 AQ7543 F- zzz::Tn10 Recommended medium:
Luria broth supplemented with glucose (0.1%). A mixture of 7 strains 30139 ME9379 AQ7676 F- ΔlacX74 hsdR mcrB araD Δ(araABC-leu)7679 galU galK rpsL thi Recommended medium:
Luria broth supplemented with glucose (0.1%). same as MC1061: Meissner et al., 1987, Proc. natl. Acad. Sci. USA., 84, 4171-4175. 30113 ME9381 AQ7678 F- recD1903::miniTc Recommended medium:
Luria broth supplemented with glucose (0.1%). same as DBP271: Biek D. P. and Cohen S. N., 1986, J. Bacteriol., 167, 594-603.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30113 ME9382 AQ7679 F- recD1903::miniTc priA1::kan Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml) Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.

30113 ME9383 AQ7763 F- deoB(or C) recD1903::miniTc metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 thyA AQ7678 AQ4241 TD./PI T4 gene2-, recD1903 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30130 ME9385 AQ7805 F- thyA zif::pML31(Kan-r) oriCdel-1071 his-4 trp-3 metE46 thi-1 galK2 lacY1(or lacZ4) mtl-1 recD1903::miniTc ara-9 deoB(or C) tsx-3 ton-1 rpsL8(or 9) supE44 AQ7678 AQ7797 TD./PI recD1903::miniTc Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
30128 ME9386 AQ7946 F- ruvC51 eda-51::Tn10 Recommended medium:
Luria broth supplemented with glucose (0.1%). same as TNM554: Sharples et al., 1990, Mol. Gen. Genet., 221, 219-226.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30123 ME9387 AQ7960 F- metB1 trpA9605 his-29 proB(or C) ilv thyA deoB(or C) ruvC51 eda51::Tn10 AQ7946 AQ2634 TD./PI UV-s, ruvC51 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30132 ME9390 AQ7996 F- fis::Km rpsL thi ara Δ(lac-pro) Recommended medium:
Luria broth supplemented with glucose (0.1%), kanamycin (55μg/ml). same as WM2016: Koch et al., 1988, Proc. Natl. Acad. Sci. USA., 85, 4237-4241. 30114 ME9391 AQ7998 F- himA::Tc himD::Cm thi ara Δ(lac-pro) Recommended medium:
Luria broth supplemented with glucose (0.1%), tetracycline (20μg/ml). same as WM2017: Koch et al., 1988, Proc. Natl. Acad. Sci. USA., 85, 4237-4241. 30146 ME9392 AQ8007 F- sup(am) recF332::Tn3 rpsL Δ(lac)U169 AQ2285 AQ4063 TD./PI recF332::Tn3 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9393 AQ8014 F- λ+ λp[sfiA::lacZ cI(Ind-)] thyA deoB(or C) xyl-5 ΔrecA306 leuB6 thr-1 his-4 argE3 ilv(ts) Δlac λp[sfiA::lacZ cI(Ind-)] supE44 sfiA211 galK2 mtl-1 srl::Tn10 ara-15 tsx33 rpsL31 AQ7982 Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). 30132 ME9394 AQ8017 F- ilv trpA9605 his-29 pro metB1 thyA deoB(or deoC) zga::Tn10 recB21 AQ5931 SPONTANEOUS thyA Thy-(trimethoprim selection) Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30142 ME9395 AQ8022 F- recJ284::dTn10(Tc-s) trpA9605 his-29 proB(or C) ilv metB1 thyA deoB(or C) AQ3362 OTHER METHODS Bochner selection recJ284::dTn10(Tc-s) Tc-(Bochner selection) Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9396 AQ8024 F- thyA rnhA224 deoB (or C) ruvC51 eda-51::Tn10 trpA9605 his-29 pro ilv metB1 AQ7946 AQ666 TD./PI UV-s, ruvC51 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
30127 ME9399 AQ8046 F- trpA9605 recB270(Ts) zga::Tn10 deoB(or C) thyA metB1 ilv pro his-29 rnhA339::cat AQ4432 AQ8033 TD./PI UVts, recB270 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
30134 ME9400 AQ8058 F- his-29 zga::dTn10(Tc-s) recB270(Ts) rnhA339::cat deoB(or C) thyA metB1 ilv pro trpA9605 AQ8046 OTHER METHODS Bochner selection zga::dTn10(Tc-s) Tc-s(Bochner selection) Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
30134 ME9402 AQ8070 F- recJ284::dTn10(Tc-s) deoB(or C) thyA- metB1 ilv pro his-29 trpA9605 recD1903::miniTc AQ7678 AQ8022 TD./PI UV-s, recD1903::miniTc Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9403 AQ8072 F- zga::Tn10 trpA9605 his-29 pro ilv metB1 thyA deoB(or C) recB21 AQ8045 AQ634 TD./PI UV-s, recB21 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9404 AQ8074 F- recB270(Ts) zga::dTn10(Tc-s) thyA metB1 ilv pro his-29 trpA9605 dnaA850::Tn10 rnhA339::cat deoB(or C) AQ3519 AQ8058 TD./PI Ts, dnaA850::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
30135 ME9405 AQ8101 F- thyA metB1 dnaA46 his-29 trpA9605 pro deoB/C rnhA339::cat AQ3519 AQ705 TD./PI Tr, rnhA339::cat Cm-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30113 ME9406 AQ8107 F- λ+ λp[sfiA::lac cI(Ind-)] λp[sfiA::lac cI(Ind-)] rpsL Δ(pro-lac) pro Recommended medium:
Luria broth supplemented with glucose (0.1%). same as GC4597: Huisman O. and D'Ari R., 1981, Nature, 290, 797-799. 30134 ME9407 AQ8110 Other λ+ λp[sfiA::lacZ cI(Ind-)] supE44 sfiA211 galK2 ara-15 xyl-5 mtl-1 srl::Tn10 argE3 rpsL31 thyA deoB(or C) his-4 leuB6 thr-1 ΔrecA306 tsx33 ilv(ts) Δlac λp[sfiA::lacZ cI(Ind-)] AQ8014 TF. miniF-recA428 Km-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30132 ME9409 AQ8115 F- Other λ+ λp[sfiA::lacZ+ cI(Ind-)] thr-1 Δ(srl-recA)306 leuB6 his4 argE3 ilv(ts) Δlac λp[sfiA::lacZ+ cI(Ind-)] supE44 sfiA211 galK2 ara-15 xyl-5 mtl-1 srl::Tn10 tsx-33 rpsL31 thyA deoB(or C) AQ8014 TF. miniF-recA+ Km-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30132 ME9410 AQ8130 F- trpA9605 recG258::miniTn10kan deoB(or C) thyA metB1 ilv pro his-29 AQ8034 AQ634 TD./PI UV-s, recG258 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
30132 ME9411 AQ8134 F- deoB(or deoC) trpA9605 his-29 pro ilv metB1 thyA recD1903::miniTc AQ8070 AQ634 TD./PI T4 gene 2 sensitive, recD1903 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
ME9412 AQ8135 F- rnhA224 thyA metB1 ilv pro his-29 trpA9605 recD1903::miniTc deoB (or deoC) AQ8070 AQ666 TD./PI T4 gene2 sensitive, recD1903 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
30134 ME9413 AQ8136 F- λ+ λp[sfiA::lac cI(Ind-)] rpsL λp[sfiA::lac cI(Ind-)] rnhA339::cat Δ(pro-lac) pro AQ3519 AQ8017 TD./PI rnhA339::cat Cm-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9415 AQ8146 F- thyA trpA9605 his-29 pro ilv metB1 deoB (or deoC) ruvC51 eda51::Tn10 recG258::miniTn10kan AQ8034 AQ7960 TD./PI UVs(<<10J), recG258 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30123 ME9417 AQ8164 F- λ+ λp[sfiA::lac cI(Ind-)] pro Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] rnhA339::cat recD1903::miniTc AQ8070 AQ8136 TD./PI T4 gene2 sensitive, recD1903 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9421 AQ8208 F- ilv recG258::miniTn10Km metB1 recD1903::miniTc thyA deoB(or deoC) trpA9605 his-29 pro AQ8034 AQ8134 TD./PI UVs, recG258 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30132 ME9422 AQ8224 F- recN1502::Tn5 his-4 xyl5 mtl-1 kdg51 supE44 tsx-33 lacY1 galK2 rpsL31 thi-1 thr-1 proA2 leuB6 argE3 ara14 Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as RDK1041: Lovett et al., 1988, Genetics, 120, 37-45.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30139 ME9424 AQ8272 F- λ+ λp[sfiA::lac cI(Ind-)] pro srlC300::Tn10 recA56 rnhA339::cat λp[sfiA::lac cI(Ind-)] rpsL Δ(pro-lac) AQ536 AQ8136 TD./PI UVs, recA56 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9425 AQ8273 F- λ+ λp[sfiA::lac cI(Ind-)] rnhA224 λp[sfiA::lac cI(Ind-)] rpsL Δ(pro-lac) pro recD1903::miniTc T4 gene2 sensitive, recD1903 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9426 AQ8300 F- lacY1 thr-1 leu-6 thi-1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 lexA3 malF3089::Tn10 AQ6471 AQ157 TD./PI UVs, lexA3 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9427 AQ8307 F- λ+ λp[sfiA::lac cI(Ind-)] rpsL λp[sfiA::lac cI(Ind-)] rnhA339::cat lexA3 malF3089::Tn10 pro Δ(pro-lac) AQ8300 AQ8136 TD./PI UVs, lexA3 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9428 AQ8326 F- deoB (or deoC) trpA9605 his-29 pro recJ284::dTn10(Tc-s) rnhA224 zag::Tn10 ilv metB1 thyA AQ978 AQ8022 TD./PI Ls, rnhA224 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
30127 ME9429 AQ8330 F- λ+ λp[sfiA::lac cI(Ind-)] rpsL λp[sfiA::lac cI(Ind-)] rnhA339::cat recJ284::Tn10 Δ(pro-lac) pro AQ3362 AQ8136 TD./PI UVs, recJ284 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9430 AQ8353 F- λ+ λp[sfiA::lac cI(Ind-)] λp[sfiA::lac cI(Ind-)] recG258::miniTn10kan pro Δ(pro-lac) rpsL AQ8034 AQ8107 TD./PI UVs, recG258 Km-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30123 ME9438 AQ8474 F- rnhA339::cat pro thyA metB1 ilv trpA9605 his-29 deoB (or deoC) srlC300::Tn10 recA200 zga::dTn10(Tc-s) recB270(Ts) AQ751 AQ8058 TD./PI UVs(Ts), recA200 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Ts, Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30134 ME9439 AQ8478 F- λ+ λp[sfiA::lac cI(Ind-)] rnhA339::cat pro Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] recF332::Tn3 AQ8007 AQ8136 TD./PI UVs, recF332 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9440 AQ8488 F- trpA9605 srlC300::Tn10 recA200 zga::dTn10(Tc-s) recB270(Ts) deoB(or deoC) thyA metB1 ilv his-29 AQ751 AQ8463 TD./PI UVs(Ts), recA200 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9441 AQ8489 F- recA200 trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) rnhA339::cat srlC300::Tn10 AQ751 AQ8032 TD./PI UVs(Ts), recA200 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30134 ME9442 AQ8494 F- thyA recB270(Ts) zga::dTn10(Tc-s) recJ284::Tn10 ilv pro his-29 trpA9605 metB1 deoB (or deoC) rnhA339::cat AQ3362 AQ8058 TD./PI UVs, recJ284 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30134 ME9443 AQ8501 F- λ+ λp[sfiA::lac cI(Ind-)] λp[sfiA::lac cI(Ind-)] Δ(pro-lac) pro rpoB5501 rnhA339::cat rpsL AQ8136 MUTAGENESIS/Others EMS rpoB5501 Rif-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30134 ME9444 AQ8505 F- λ+ λp[sfiA::lac cI(Ind-)] rpsL pro Δ(pro-lac) λp[sfiA::lac cI(Ind-)] rnhA339::cat rpoB5505 AQ8136 MUTAGENESIS/Others EMS rpoB5505 Rif-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9448 AQ8534 F- zih-35::Tn10 polA25::spc AQ6466 AQ8530 TD./PI UV-s, polA25 Tc-r, Spc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30130 ME9449 AQ8542 F- λ+ λp[sfiA::lac cI(Ind-)] λp[sfiA::lac cI(Ind-)] rpsL Δ(pro-lac) pro recN261 tyrA::Tn10 rnhA339::cat AQ2987 AQ8136 TD./PI UVs, recN261 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30134 ME9450 AQ8560 F- his-29 pro ilv metB1 thyA deoB(or deoC) polA25::spc zih-35::Tn10 trpA9605 AQ8534 AQ634 TD./PI UVs, polA25 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), spectinomycin (40μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30130 ME9452 AQ8583 F- λp[sfiA::lac cI(Ind-)] rnhA339::cat λp[sfiA::lac cI(Ind-)] rpsL Δ(pro-lac) pro rpoB2 AQ8564 AQ8136 TD./PI Rif-r, rpoB2 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9453 AQ8608 F- λ+ λp[sfiA::lac cI(Ind-)] rpsL λp[sfiA::lac cI(Ind-)] rpoB3406 rnhA339::cat pro Δ(pro-lac) AQ8575 AQ8136 TD./PI Rif-r, rpoB3406 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9454 AQ8619 F- polA25::spc metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 sfiA11 lexA71::Tn5 zih-35::Tn10 AQ8534 AQ3476 TD./PI UV-s, polA25 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9456 AQ8622 F- λ+ λp[sfiA::lac cI(Ind-)] pro rpsL λp[sfiA::lac cI(Ind-)] rnhA339::cat rpoB3595 Δ(pro-lac) AQ8580 AQ8136 TD./PI Rif-r, rpoB3595 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9457 AQ8627 F- λp[sfiA::lac cI(Ind-)] λp[sfiA::lac cI(Ind-)] pro rnhA339::cat Δ(pro-lac) rpoB8 rpsL AQ8566 AQ8136 TD./PI Rif-r, rpoB8 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9460 AQ8654 F- λp[sfiA::lac cI(Ind-)] Δ(pro-lac) rpoB2 λp[sfiA::lac cI(Ind-)] rpsL pro AQ8564 AQ8107 TD./PI Rif-r, rpoB2 Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9461 AQ8656 F- λ+ λp[sfiA::lac cI(Ind-)] pro Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] rpoB3595 AQ8580 AQ8107 TD./PI Rif-r, rpoB3595 Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9462 AQ8658 F- Other λ+ λp[sfiA::lac cI(Ind-)] λp[sfiA::lac cI(Ind-)] rnhA339::cat pro Δ(pro-lac) rpsL rpoB2 AQ8583 TF. pBR322 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9463 AQ8660 F- Other λ+ λp[sfiA::lac cI(Ind-)] rnhA339::cat pro Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] rpoB2 AQ8583 TF. pSK760 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9464 AQ8685 F- Other λp[sfiA::lac cI(Ind-)] pro rpoB3595 rnhA339::cat λp[sfiA::lac cI(Ind-)] rpsL Δ(pro-lac) AQ8622 TF. pBR322 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9465 AQ8687 F- Other λ+ λp[sfiA::lac cI(Ind-)] rpoB3595 pro Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] rnhA339::cat AQ8622 TF. pSK760 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9466 AQ8695 F- sfiA11 lexA71::Tn5 polA25::spc zih-35::dTn10(Tc-s) azi-9 rpsL171 lacZ118 lacI22 trpA9605 metE90 nalA18 thyA708 deo-29 rnhA102 recA200 AQ8619 OTHER METHODS Bochner selection zih-35::dTn10(Tc-s) Tc-s Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9467 AQ8718 F- sfiA11 metE90 trpA9605 trpR55 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 Δ(srlR-recA)306 lexA71::Tn5 AQ663 AQ3476 TD./PI UVs, recA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9468 AQ8720 F- lac+ ilv(ts) sulA221 lexA71::Tn5 malF3089::Tn10 recA441 ara-14 lac xyl-5 mtl-1 tsx-33 supE44 thr-1 leuB6 Δ(gpt-proA)62 his-4 argE3 thi-1 rpsL31 galK2 AQ6471 AQ4414 TD./PI lexA71::Tn5 malF3089::Tn10 TC-r, Km-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9469 AQ8747 F- azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 lexA71::Tn5 sfiA11 polA25::spc zih-35::dTn10(Tc-s) tnaA::Tn10 dnaA5 rpsL171 lacZ118 lacI22 trpA9605 metE90 AQ3364 AQ8694 TD./PI Ts, dnaA5 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9470 AQ8763 F- lacZ118 lacI22 trpR55 trpA9605 metE90 rnhA102 deo-29 thyA708 nalA18 azi-9 rpsL171 Δ(srlR-recA)306 AQ663 AQ377 TD./PI UVs, ΔrecA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30118 ME9471 AQ8809 F- metB1 pro his-29 trpA9605 zig::Tn10 polA1 deoB(or deoC) thyA ilv AQ1409 AQ634 TD./PI UVs, polA1 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30130 ME9472 AQ8817 F- rpsL171 nalA18 thyA708 deo-29 rnhA102 recA200 lexA71::Tn5 sfiA11 polA25::spc zih-35::dTn10(Tc-s) tnaA::dTn10(Tc-s) dnaA5 metE90 trpA9605lacI22 lacZ118 azi-9 AQ8747 OTHER METHODS Bochner selection tnaA::dTn10(Tc-s) Tc-s Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9473 AQ8820 F- proC::Tn5 trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) Δ(lacIPOZY)x74 AQ2013 AQ634 TD./PI Lac-, Δ(lacIPOZY)x74 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30142 ME9474 AQ8825 F- zih-35::Tn10 trpA9605 lacZ118 metE90 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 lexA71::Tn5 sfiA11 tnaA::dTn10(Tc-s) dnaA5 lacI22 AQ6466 AQ8817 TD./PI UVr, polA+ Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9476 AQ8850 F- tsx IN(rrnD-rrnE)1 tus::kan lacY(?) pro-2 arg427 thyA deoB or C trpA9605(Am) trpR ilv (Am) his-29 from T. Hill. Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as TH210 30127 ME9477 AQ8851 F- trpA9605 his-29 metB1 ilv priA1::kan pro thyA deoB (or deoC) AQ8845 AQ634 TD./PI filamentous, priA1 Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
3211, 30113 ME9479 AQ8862 F+ F' supE44 supF58 HsdR514 galK2 galT22 metB1 trpR55 lacY1 Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). 30131 ME9481 AQ8864 F- F' trpR55 metB1 galT22 galK2 lacY1 or Δ(lacIZY)6 supF58 supE44 hsdR514(r- m+) Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). same as RM2427 (CJ222): Lifsics et al., 1992, J. Bacteriol., 174, 6965-6973. 30131 ME9482 AQ8865 F+ F' metB1 galK2 galT22 supF58 supE44 hsdR514(r- m+) trpR55 lacY1 or Δ(lacIZY)6 Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). same as RM2428 (CJ251): Lifsics et al., 1992, J. Bacteriol., 174, 6965-6973. 30131 ME9484 AQ8867 F- metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 recA200 sfiA11 lexA71::Tn5 polA25::spc zih-35::dTn10(Tc-s) zag::Tn10 AQ979 AQ8695 TD./PI cSDR-, rnhA+ Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9485 AQ8869 F- sfiA11 polA25::spc zih-35::dTn10(Tc-s) metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 AQ8633 OTHER METHODS Bochner selection zih-35::dTn10(Tc-s) Tc-s Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9486 AQ8876 F- lacI22 tnaA::dTn10(Tc-s) dnaA5 sfiA11 lexA71::Tn5 rnhA102 deo-29 nalA18 azi-9 rpsL171 lacZ118 polA25::spc trpA9605 metE90 AQ6375 AQ8817 Tr, recA+ Thy+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9487 AQ8877 F- metE90 zih-35::dTn10(Tc-s) polA25::spc lexA71::Tn5 zag::dTn10(Tc-s) sfiA11 recA200 deo-29 thyA708 nalA18 azi-9 rpsL171 lacZ118 lacI22 trpA9605 AQ8867 OTHER METHODS Bochner selection zag::dTn10(Tc-s) Tc-s Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9489 AQ8882 F- rnhA102 metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 sfiA11 zih-35::dTn10(Tc-s) AQ6375 AQ8869 CROSS UVr, recA+ Thy+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9490 AQ8885 F- zag::dTn10(Tc-s) malF3089::Tn10 trpA9605 his-29 thyA deoB (or deoC) metD88 lac-del3 ilv dnaA5 recA200 polA25::spc AQ6471 AQ8877 TD./PI Km-s, lexA+ Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Ts
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30130 ME9491 AQ8886 F- sfiA11 lexA71::Tn5 polA25::spc zag::dTn10(Tc-s) Δ(srlR-recA)306 srl::Tn10 zih-35::dTn10(Tc-s) metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 recA200 AQ8115 AQ8877 TD./PI UVs, ΔrecA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9492 AQ8901 F- mtl-1 metE46 trp-3 his-4 thi-1 galK2 lacY1 (or lacZ4) ara-9 tsx-3 ton-1 rpsL8 (or rpsL9) supE44 thyA zga::dTn10(Tc-s) rnhA339::cat AQ3519 AQ8880 TD./PI rnhA339::cat Cm-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30135 ME9493 AQ8926 F- malF3089::Tn10 metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 sfiA11 zih-35::dTn10(Tc-s) lexA71::Tn5 AQ8720 AQ8882 TD./PI Km-r, lexA71::Tn5 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9494 AQ8931 F- recA200 tus::kan rnhA224 deoB (or deoC) thyA metB1 ilv pro trpA9605 AQ8850 AQ2787 TD./PI tus::kan Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30127 ME9496 AQ8939 F- rpsL lac thiA thi thy metB1 zae-502::Tn10 dnaE293 AQ6386 AQ548 TD./PI Ts, dnaE293 Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30131 ME9497 AQ8945 F- metB1 pro trpA9605 ilv dnaA850::Tn10 rnhA224 deoB (or deoC) recA200 thyA AQ3519 AQ2787 TD./PI dnaA850::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30127 ME9498 AQ8948 F- trpA9605 pro ilv metB1 thyA recA200 deoB (or deoC) rnhA224 tus::kan dnaA850::Tn10 AQ3519 AQ8931 TD./PI dnaA850::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30127 ME9500 AQ8967 F- Δ(srlR-recA)306 deoB (or deoC) thyA metB1 ilv pro his-29 trpA9605 AQ8115 AQ634 TD./PI UVs, ΔrecA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30142 ME9501 AQ8969 F- Δ(srlR-recA)306 trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) rnhA224 AQ8115 AQ666 TD./PI UVs, ΔrecA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30127 ME9502 AQ8980 F- dnaE293 zae-502::Tn10 rnhA102 deo-29 recA200 sfiA11 lexA71::Tn5 thyA708 nalA18 azi-9 rpsL171 lacZ118 lacI22 trpA9605 metE90 AQ8939 AQ3476 TD./PI Ts, dnaE293 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30131 ME9503 AQ8996 F- polA25::spc metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 sfiA11 lexA71::Tn5 zih-35::dTn10(Tc-s) Δ(srlR-recA)306 srlR301::Tn10 AQ663 AQ8695 TD./PI ΔrecA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9504 AQ9010 F- tsx-3 ton-1 rpsL8 (or rpsL9) supE44 thyA zga::dTn10(Tc-s) rnhA339::cat dnaA850::Tn10 metE46 trp-3 his-4 thi-1 galK2 lacY1 (or lacZ4) mtl-1 ara-9 AQ3519 AQ8901 TD./PI dnaA850::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30135 ME9505 AQ9013 F- trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) ΔpolA::kan AQ8866 AQ634 TD./PI Ls, ΔpolA::kan Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9506 AQ9016 F- F' trpR55 hsdR514(r- m+) ΔpolA::kan zih-35::Tn10 supF58 lacY1 or Δ(lacIZY)6 galK2 galT22 metB1 supE44 AQ6466 AQ8866 TD./PI UVs, ΔpolA::kan Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30131 ME9509 AQ9045 F- azi-9 metE90 trpA9605 lacI22 lacZ118 rpsL171 nalA18 thyA708 deo-29 rnhA102 recA200 lexA71::Tn5 sfiA11 tnaA::dTn10(Tc-s) dnaA5 ΔpolA::kan zih-35::Tn10 AQ9016 AQ8937 TD./PI Ls, ΔpolA Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30131 ME9510 AQ9077 F- oriCdel-1071 rpsL8 (or rpsL9) supE44 thyA zga::dTn10(Tc-s) rnhA339::cat recB270(Ts) zif90::pML31 metE46 trp-3 his-4 thi-1 galK2 lacY1 (or lacZ4) mtl-1 ara-9 tsx-3 ton-1 AQ3634 AQ8964 TD./PI Ts, recB270 Thy+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30135 ME9513 AQ9118 F- λ+ λp[sfiA::lac cI(Ind-)] pro Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] rnhA339::cat rpoB3595 Δ(srlR-recA)306 AQ8115 AQ9098 UVs, ΔrecA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9514 AQ9142 F- λ+ λp[sfiA::lac cI(Ind-)] pro Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] rnhA339::cat rpoB3595 lexA3 malF3089::Tn10 AQ8300 AQ9098 TD./PI UVs, lexA3 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30129 ME9517 AQ9179 F- Other trpA9605 metB1 deoB (or deoC) thyA pro ilv his-29 Δ(srlR-recA)306 rnhA224 AQ8969 TF. pAQ7985 (miniF::recA730) Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30127 ME9522 AQ9232 F- ΔpolB::spc Str-r thi Δ(lac-pro) ara From M. F. Goodman. Recommended medium:
Luria broth supplemented with glucose (0.1%). same as SH2101
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30130 ME9523 AQ9237 F- metE90 sfiA11 rnhA102 deo-29 thyA708 nalA18 azi-9 rpsL171 lacZ118 lacI22 trpA9605 AQ6466 AQ8752 TD./PI UVr, recA+ Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9524 AQ9239 F- ara zac-3051::Tn10 ΔpolB::spc thi Δ(lac-pro) AQ6384 AQ9232 TD./PI zac-3051::Tn10 Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.

Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.

Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.

Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.

Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30130 ME9526 AQ9250 F- AQ8937 rnhA102 metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 recA200 lexA71::Tn5 sfiA11 tnaA::dTn10(Tc-s) dnaA5 zih-35::dTn10(Tc-s) ΔpolB::spc zac-3051::Tn10 AQ9239 AQ8937 TD./PI Spc-r, ΔpolB Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9527 AQ9262 F- ruvA60::Tn10 Recommended medium:
Luria broth supplemented with glucose (0.1%). same as N2057: Shaples et al., 1990, Mol. Gen. Genet., 221, 219-226.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30123 ME9530 AQ9293 F- Other his-29 pro ilv trpA9605 thyA deoB (or deoC) priA1::kan metB1 AQ9215 TF. pET3c-K230R Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30139 ME9531 AQ9298 F- recA200 lexA71::Tn5 sfiA11 tnaA::dTn10(Tc-s) dnaA5 recF322::Tn3 trpA9605 metE90 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 AQ4790 AQ8937 TD./PI recF322::Tn3 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30130 ME9532 AQ9317 F- trpA9605 ruvA60::Tn10 deoB (or deoC) thyA metB1 ilv pro his-29 AQ9262 AQ634 TD./PI UVs, ruvA60 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30123 ME9533 AQ9334 F- recF322::Tn3 zih-35::dTn10(Tc-s) polA25::spc lexA71::Tn5 sfiA11 recA200 rnhA102 deo-29 metE90 thyA708 nalA18 azi-9 rpsL171 lacZ118 lacI22 trpA9605 AQ4791 AQ8695 TD./PI recF322::Tn3 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9534 AQ9336 F- trpA9605 his-29 pro ilv metB1 thyA deoB(or C) recG258::miniTn10kan ruvA60::Tn10 AQ9262 AQ8130 TD./PI UVs, ruvA60 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30123 ME9535 AQ9349 F- ruvA60::Tn10 rnhA224 deoB (or deoC) thyA metB1 ilv pro his-29 trpA9605 AQ9262 AQ666 TD./PI UVs, ruvA60 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30127 ME9536 AQ9352 F- rnhA102 recA200 sfiA11 recF322::Tn3 metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 AQ4790 AQ3474 TD./PI UVs, recF322 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30118 ME9539 AQ9436 F- λ+ λp[sfiA::lac cI(Ind-)] λp[sfiA::lac cI(Ind-)] ruvA60::Tn10 rpsL Δ(pro-lac) pro AQ9262 AQ8107 TD./PI UVs, ruvA60 Tc-r Recommended medium:
Luria broth supplemented with glucose (0.1%). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30123 ME9540 AQ9438 F- pro Δ(pro-lac) ruvA60::Tn10 λp[sfiA::lac cI(Ind-)] rpsL recG258::miniTn10kan AQ9262 AQ8353 UVs, ruvA60 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30123 ME9541 AQ9450 F- his-4 thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 argE3 rpsL-31 tsx-33 supE44 Δ(srlR-recA)306 srlC300::Tn10 AQ7982 AQ8846 TD./PI UVs, ΔrecA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30122 ME9542 AQ9504 F- λ+ λp[sfiA::lacZ cI(Ind-)] thyA deoB (or deoC) Δ(lacIPOZY)x74 proC::Tn5 λp[sfiA::lacZ cI(Ind-)] trpA9605 his-29 pro ilv metB1 λ[sfiA::lacZ] AQ8820 OTHER METHODS TD./Others PENICILLIN λ infection λp[sfiA::lacZ cI(Ind-)] Lac+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30142 ME9543 AQ9507 F- IN(rrnD-rrnE)1 lacZ53 rpsL151 thyA36 polA12 rha-5 deoC2 Δ(srlR-recA)306 AQ663 AQ6039 TD./PI UVs, ΔrecA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30118 ME9544 AQ9511 F- λ+ λGM108A(rnh+ dnaQ::lacZ) recA200 sfiA11 lexA71::Tn5 polA25::spc zih-35::Tn10 λGM108A(rnh+ dnaQ::lacZ) metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 λGM108A AQ8619 OTHER METHODS λ infection λGM108A(rnh+ dnaQ::lacZ) Blue colony on X-gal plate Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9545 AQ9513 F- λ+ λGM108A (rnhA+ dnaAQ::lacZ) lacI22 recA200 sfiA11 polA25::spc zih-35::Tn10λGM108A (rnhA+ dnaAQ::lacZ) deo-29 thyA708 nalA18 azi-9 rpsL171 lacZ118 rnhA102 trpA9605 metE90 λGM108A AQ8633 OTHER METHODS λ infection λGM108A (rnhA+ dnaAQ::lacZ) Blue colony on X-gal plate Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9546 AQ9552 F- Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). same as MG1655: Singer et al., 1989, Micobiol. Rev., 53, 1-24. 30114 ME9547 AQ9555 F- gidA95::kan AQ7664 AQ9552 TD./PI gidA95::kan Km-r Recommended medium:
Luria broth supplemented with glucose (0.1%). 30114 ME9548 AQ9561 F- thr-1 recB21 supE44 tsx-33 rpsL31 argE3 his-60 proA2 recC22 sbcA23 Δ(srlR-recA)306 srlC300::Tn10 mtl-1 xyl-5 ara-4 galK2 thi-1 lacY1 leuB6 AQ7982 AQ3625 TD./PI UVs, ΔrecA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30122 ME9549 AQ9601 F- thi-1 thr-1 leu-6 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA recD1903::miniTc AQ8070 AQ5004 TD./PI recD1903 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30122 ME9550 AQ9643 F- λ+ λDB101(gidA+) trpA9605 asnA101::cat deoB (or deoC) thyA metB1 ilv pro his-29 λDB101 AQ7664 OTHER METHODS λ infection Km-s, gidA+ Cm-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30114 ME9552 AQ9648 F- asn101::cat AQ9643 AQ9555 TD./PI Km-s, gidA+ Cm-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml). 30146 ME9556 AQ9717 F- galK2 lacY1 ara-14 xyl-5 mtl-1 kdg-51 supE44 tsx-33 thyA zga::Tn10 deoB (orC) rpsL31 thi-1 thr-1 proA2 leuB6 argE3 his-4 recN1502::Tn5 AQ5786 AQ8224 TD./PI low thymide, thyA Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30122 ME9557 AQ9747 F- thyA thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 deoB (orC) lacZU118 lacI42::Tn10 AQ6389 AQ5004 TD./PI Lac-, lacZU118 lacI42::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30122 ME9561 AQ9806 F- mtl-1 proA2 his-60 argE3 rpsL31 tsx-33 supE44 recB21 recC22 sbcA23 priA1::kan thr-1 leuB6 thi-1 lacY1 galK2 ara-4 xyl-5 AQ8845 AQ3625 TD./PI filamentous, priA1 Km-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30139 ME9562 AQ9826 F- thyA708 polA25::spc zih-35::dTn10(Tc-s) recQ61::Tn3 deo-29 rnhA102 recA200 sfiA11 lexA71::Tn5 metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 AQ9176 AQ8695 TD./PI recQ61::Tn3 Ap-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9563 AQ9833 F- Other λ+ λ[sfiA::lacZ cI(ind-)] thyA thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 deoB (orC) lacZU118 lacI42::Tn10 λp[sfiA::lacZ cI(Ind-)] AQ9789 TF. pHKχ1-cos, pFM123 Km-r, Ap-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml), kanamycin (55μg/ml). cultured at 30C
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30122 ME9565 AQ9857 F- nalA18 rnhA102 thyA708 ruvA60::Tn10 zih-35::dTn10(Tc-s) polA25::spc lexA71::Tn5 sfiA11 recA200 deo-29 metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 AQ9262 AQ8694 TD./PI ruvA60::Tn10 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30118 ME9569 AQ9897 F- Other his-29 rnhA339::cat recG258::miniTn10kan deoB (or deoC) thyA metB1 ilv pro trpA9605 AQ9853 TF. Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30116 ME9572 AQ9950 F- araD139 lrp-201::Tn10 thi rpsL Δ(lacIPOZYA)U169 Recommended medium:
Luria broth supplemented with glucose (0.1%). same as RO64: Lange et al., 1993, J. Bacteriol., 175, 7910-7917. 30142, 35355 ME9573 AQ9964 F- recG258::miniTn10kan trpA9605 his-29 pro ilv metB1 thyA deoB(or C) Δ(srlR-recA)306 srlC300::Tn10 AQ663 AQ8130 TD./PI UVs, ΔrecA306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30116 ME9574 AQ9999 F- trpA9605 metB1 thyA deoB(or deoC) his-29 pro ilv lrp-201::Tn10 AQ9950 AQ634 TD./PI lrp-201 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). 30142 ME9575 AQ10033 F- gidA95::kan rnhA224 deoB (or deoC) thyA metB1 ilv trpA9605 his-29 pro AQ7664 AQ666 TD./PI gidA95::kan Km-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30114 ME9576 AQ10051 F- rnhA224 Δ(srlR-recA)306 deoB (or deoC) thyA metB1 ilv pro his-29 trpA9605 AQ663 AQ666 TD./PI UVs, Δ(srlR-recA)306 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30116 ME9578 AQ10070 F- zic-4901::Tn10 recG162 AQ6460 AQ9947 TD./PI UVs, recG162 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30116 ME9579 AQ10071 F+ priA2::kan Recommended medium:
M9 salts glucose medium supplemented with Casamino acids (0.2%), Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). same as PN105: Nurse et al., 1991, J. Bacteriol., 173, 6686-6693.
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30139 ME9580 AQ10075 F- ilv metB1 pro his-29 trpA9605 deoB (or deoC) zig::Tn10 thyA polA12 recG258::miniTn10kan AQ1524 AQ8130 TD./PI Ts, UVs, polA12 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30116 ME9582 AQ10092 F- ilv trpA9605 his-29 pro metB1 thyA deoB (or deoC) recG162 zic::Tn10 AQ10070 AQ634 TD./PI UVs, recG162 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30116 ME9583 AQ10119 F- tnaA600::Tn10 trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) recG258::miniTn10kan dnaA46 AQ5456 AQ8130 TD./PI Ts, dnaA46 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30116 ME9586 AQ10180 F- λ+ λp[sfiA::lac cI(Ind-)] λp[sfiA::lac cI(Ind-)] recG162 zic::Tn10 pro Δ(pro-lac) rpsL AQ10070 AQ8107 TD./PI UVs, recG162 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30116 ME9588 AQ10303 F- fis::kan thi rnhA224 ara Δ(lac-pro) rpsL AQ798 AQ7996 TD./PI Ls, rnh224 Tc-r, Km-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml). Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30114 ME9590 AQ10452 F- galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA sfiA11 pyrD::Tn5 lacY1 thi-1 leu-6 thr-1 AQ1023 AQ8846 TD./PI less filamentous after UV irradiation, sfiA11 Km-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), Uracil (50 μg/ml), kanamycin (55μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30139 ME9591 AQ10459 F- lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA sfiA11 thr-1 leu-6 thi-1 AQ10452 SPONTANEOUS pyrD+ Ura+ Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30139 ME9598 AQ10810 F- trpA9605 thyA metB1 ilv pro his-29 deoB (or deoC) polA1 zih-35::Tn10 AQ1409 AQ634 TD./PI UVs, polA1 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
30144 ME9599 AQ10811 F- ilv trpA9605 his-29 zih-35::Tn10 polA1 rnhA224 deoB (or deoC) thyA metB1 pro AQ1409 AQ666 TD./PI UVs, polA1 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9600 AQ10812 F- metB1 zih-35::Tn10 ilv pro his-29 trpA9605 polA25::spc deoB (or deoC) thyA AQ8534 AQ634 TD./PI UVs, polA25 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9601 AQ10814 F- his-29 trpA9605 pro ilv metB1 thyA deoB (or deoC) ΔpolB::spc zac-3051::Tn10 AQ9239 AQ634 TD./PI UVs, ΔpolB Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9602 AQ10836 F- pro his-29 trpA9605 zih-35::Tn10 polA25::spc rnhA224 deoB (or deoC) thyA metB1 ilv AQ8534 AQ666 TD./PI UVs, polA25 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9603 AQ10838 F- deoB (or deoC) ΔpolB::spc zac-3051::Tn10 trpA9605 his-29 proB (or proC) ilv metB1 thyA rnhA224 AQ9239 AQ666 TD./PI Spc-r, H2O2-s, ΔpolB::spc Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9604 AQ10850 F- his-29 trpA9605 zih-35::Tn10 polA25::spc deoB (or deoC) thyA metB1 ilv pro AQ8534 AQ634 Spc-r, polA25 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9605 AQ10884 F- ilv trpA9605 his-29 thyA deoB (or deoC) metD88 Δlac3 dnaA5 rnhA224 AQ677 AQ2547 TD./PI Tr(42C), pro+ Pro+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9606 AQ10903 F+ F' metB1 deoB (or deoC) ΔpolA::kan trpA9605 his-29 pro ilv thyA AQ8862 AQ9013 F'-duction F' polA+ Tc-r, Cm-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9607 AQ10904 F+ F' his-29 pro trpA9605 ΔpolA::kan deoB (or deoC) thyA metB1 ilv AQ8863 AQ9013 F'-duction F' polA 5'->3' exo Tc-r, Cm-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9608 AQ10905 F+ F' his-29 ΔpolA::kan deoB (or deoC) thyA metB1 ilv proB (or proC) trpA9605 AQ8864 AQ9013 F'-duction F' klenow Tc-r, Cm-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9609 AQ10907 F- thyA trpA9605 his-29 zig::Tn10 polA1 rnhA224 dnaA5 ilv Δlac3 metD88 deoB (or deoC) AQ1409 AQ10884 TD./PI UVs, polA1 Tc-r Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9611 AQ10924 F+ F' metB1 trpA9605 his-29 pro ilv thyA deoB (or deoC) rnhA224 AQ8865 AQ666 F'-duction Lac+, F' vector Cm-r Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9612 AQ10925 F+ F' rnhA224 deoB (or deoC) thyA metB1 ilv pro his-29 trpA9605 AQ8862 AQ666 F'-duction F' polA+ Cm-r, Lac+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9613 AQ10926 F+ F' rnhA224 deoB (or deoC) thyA metB1 ilv pro his-29 trpA9605 AQ8863 AQ666 F'-duction F' exo Cm-r, Lac+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9614 AQ10927 F+ F' trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) rnhA224 AQ8864 AQ666 F'-duction F' klenow Cm-r, Lac+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9615 AQ10928 F+ F' trpA9605 rnhA224 pro deoB (or deoC) thyA metB1 ilv his-29 AQ8866 AQ666 F'-duction F' D355A D357A Cm-r, Lac+ Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9616 AQ10955 F+ F' metB1 thyA rnhA224 deoB (or deoC) his-29 trpA9605 dnaA5 zih::Tn10 polA25::spc ilv AQ8864 AQ10915 F'-duction F' klenow Cm-r, Lac+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Tr
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9617 AQ10957 F+ F' deoB (or deoC) trpA9605 his-29 argH thyA metD88 lac-del3 ilv polA25::spc zih::Tn10 AQ8863 AQ10915 F' exo Cm-r, Lac+ Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml). Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. 30144 ME9619 AQ11007 F- lacI22 rnhA102 deo-29 thyA708 nalA18 azi-9 rpsL171 metE90 lacZ118 lexA71::Tn5 sfiA11 AQ2153 AQ10984 F'-duction lexA71 Km-r Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml). Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. 30144 ME7788 SH2 F- ΔtrpE5 his W3110 ΔtrpE5 LB (37C) 29808 ME7789 SH3208 F- λ+ lambda ΔtrpE5 (λ+) his SH2 TF. lambda lysogen LB, 37C 29808 ME7790 SH3209 F- Other λ+ ΔtrpE5 his (λ) TF. pXX746 Amp-R (25 μg/ml) LB + Amp (25 μg/ml) ME7791 SH3210 F- Other λ+ lambda (λ+) his ΔtrpE5 SH3208 TF. (pXX747) Amp 25 μg/ml LB + Amp (25 μg/ml), 37C 29808 ME7792 SH3367 F- λ+ lambda (λ+) mukB106 his ΔtrpE5 SH3306 plasmid free Amp sensitive LB, 37C anucleate cell formation 29796 ME7793 SH3470 = AZ5177 F- λ+ ΔtrpE5 his mukB33 (λ) SH3459 plasmid free Amp-sensitive LB (37C) 30124 ME7794 SH9083 F- zcb::Tn10 mukB106 trpC9941 SH3910 YK1100 TD./PI mukB106 zcb::Tn10 Tet resistant, anucleate cell formation LB, 37C anucleate cell formation 30124 ME7795 SH9071 F- mukB33 zcb::Tn10 trpC9941 SH9019 YK1100 PENICILLIN TD./PI mukB33 zcb::Tn10 Tet resisrtant, anucleate cell formation LB, 37C anucleate cell formation 30124 ME7796 AZ5372 = KK47 F- trpC9941 ΔmukB::kan GC7528 YK1100 TD./PI ΔmukB::kan Kan resistant, no growth at 37C, anucleate cell formation LB, 22C temperature sensitive, anucleate cell foremation, novobiocin hypersensitive 30137, 3201 ME7797 AZ5381 = KK48 F- trpC9941 mukF::kan AZ5335 YK1100 TD./PI mukF::kan Kan resistant, no growth at 37C, anucleate cell formation LB, 22C temperature sensitive, anucleate cell formation, novobiocin hypersensitive 30137 ME7798 AZ5450 = KK62 F- trpC9941 mukE::kan AZ5449 YK1100 TD./PI mukE::kan Kan resistant, no growth at 37C, anucleate cell formation LB, 22C temperature sensitive, anucleate cell formation, novobiocin hyper sensitive 30137 ME7799 KK144 = 0T7 F- trpE61 tna-5 mukBΔ(XhoI-HpaI)::kan dadR trpA62 PB103 mukBΔ(XhoI-XhoI)::kan Kanamycin resistant (30 μg/ml) LB (22C) temperature sensitive, anucleate cell formation, novobiocin hyper sensitive 30159 ME7800 KK701 F- mukB::miniTn10-tet trpC9941 YK1100 mukB::tet Tet resistant (7.5 μg/ml), grow at 22C, but not grow 37C LB (22C) temperature sensitive, anucleate cell formation, tet-resistant, miniTn10-tet is derived from λNK1323 ME7801 BZ5 F- smbB105 (= rne) his ΔtrpE5 mukB106 (λ) SH3367 SPONTANEOUS smbB105(= rne) Growth at 42C LB, 37 C smbB105 cording for RNase E lacking a carboxyl-terminal half. 30141 ME7802 BZ31 F- (λ) his ΔtrpE5 mukB106 smbB131 (= rne) SH3367 SPONTANEOUS smbB131 (= rne) Growth at 42C LB, 37C smbB131 cording for RNase E lacking a carboxyl-terminal half. 30141 ME7803 BZ99 F- mukB106 (λl) smbB199 (= rne) his ΔtrpE5 SH3367 SPONTANEOUS smbB199 (= rne) Growth at 42C LB, 37C smbB199 cording for RNase E lacking a carboxyl-terminal half. 30141 ME7804 SH2587 F- Other hsdR sfiC supE44 gal met SH392 pXX332 Cm resistant LB + Cm, 37C 29866, 30037 ME7805 SH2588 F- Other supE44 met gal hsdR sfiC SH392 TF. pXX333 Cm resistant LB + Cm, 37C 30037, 29866 ME7806 SH2707 F- Other ilv thyA(low) recA1 lacI rpsL pro(A ore B) tyr metE trp thr KZ344 TF. pXX325 Amp resistant LB + Amp (25 μg/ml), 37C low copy plasmid, Amp should be low. 30056 ME7807 SH2708 F- Other thyA(low) recA1 lacI rpsL pro(A ore B) tyr metE trp thr ilv KZ344 pXX327 Amp resistant LB + Amp (25 μg/ml) loe copy plasmid, Amp should be low. 30056 ME7808 SH2721 = KZ344 F- metE ilv thr trp tyr pro(A ore B) rpsL lacI recA1 thyA(low) LB + thymine (37C) ME7809 SH2744 F- Other met gal hsdR sfiC supE44 sfiA::kan pyrD SH2744 pXX333 Cm resistant LB + Cm, 37C 30037, 29866 ME7810 SH2749 F- Other sfiC supE44 sfiA::kan pyrD hsdR gal met pXX332 Cm resistant LB + Cm, 37C 30037, 29866 ME7811 KK468 F- ΔmukB::kan dnaC(ts) thyA AZ5372 PC2 TF. TD./PI ΔmukB::kan Km (30 μg/ml) resistant at 22C for 4 days, anucleate cell formation by fluorescence microscopy LB + Thymine (50 μg/ml), 22C anucleate cell formation 30167 ME7812 IP101 = KK260 =KK299 rpsL trpC9941 YK1100 SPONTANEOUS rpsL Str resistant LB, 37 C 3187 ME7813 IP106 F- trpC9941 yibP::kan rprL IP101 See Ichimura et al.(2002) yibP::kan LB, 37C Not grow at 42C in LB medium (NaCl 5 g/l). Can grow at 42C in LB medium (NaCl 10 g/l). 3187 ME7814 KK465 Other trpC9941 YK1100 TF. pOT100 Amp resistant (50 μg/ml) LB + Amp (50 μg/ml), 37C The EcoRI-BamHI fragment (mini-F) of pKP2375 was jointed to the EcoRI-BamHI fragment of pBR322, yielding pOT100.
use for as DNA probe of mini-F
3195 ME7815 KK335 F- (complete dam deletion) trpC9941 dam-16::kan KK330 YK1100 TD./PI dam-16::kan (complete dam deletion) Kamycin resistant. Subcellular distribution of SeqA (by immunofluorescence microscopy) LB 37C 3197, 29815 ME7816 AZ5375 Other trpC9941 YK1100 TF. pAX850 Cm-resistant LB + Cm (37C) pAX850 carrying the smtA-mukFEB operon 30137 ME7817 AZ5376 = MY191 Other trpC9941 YK1100 pAX804 Cm LB + Cm (37C) pAX804 carrying the mukB gene 30137 ME7818 AZ5377 = MY192 F- Other trpC9941 YK1100 pKX748 Cm LB + Cm (37C) pKX748 carrying the mukF and mukE genes 30137 ME7819 AZ5378 F- Other trpC9941 YK1100 TF. pKX766 Cm LB + Cm (37C) pKX766 carrying the smtA and mukF genes 30137 ME7820 AZ5379 = MY193 F- Other trpC9941 YK1100 TF. pKX767 Cm LB + Cm (37C) pKX767 carrying the mukF gene 30137 ME7821 YM171 Other endA1 recA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F' proAB lacIqZΔM15 Tn10 (Tet-R)] XL1 blue TF. pMY105 LB + Cm, 37 C pMY105 carrying the His6-tagged mukE gene 30159 ME7822 YM198 = AZ5429 pMY105 LB + Cm, 37 C LB + Cm, 37 C pMY105 carrying the His6-tagged mukE gene 30159 ME7823 YM944 F- pMY171 LB + pMY171 carrying the His6-tagged mukF gene 30159 ME7824 YM901 pMY161 Cm + Ap LB + Cm (30 μg/ml) + Ap (100 μg/ml) pMY161 carrying the His6-tagged seqA gene 30159 ME7825 OT104 trpC9941 YK1100 = KK46 TF. pOT201 Ap (50 μg/ml) LB + Ap (50 μg/ml) pOT201 carrying the GFP-tagged seqA gene 30153 ME7826 OT106 trpC9941 ΔmukB::kan KK47 pOT201 Ap LB + Ap (50 μg/ml) pOT201 carrying the GFP-tagged seqA gene 30153 ME7827 OT114 F- (Cm-r) dam-13::Tn9 trp KK138 = KA468 pOT201 Ap LB + Ap (50 μg/ml) + Cm (7 μg/ml) pOT201 carrying the GFP-tagged seqA gene 30153 ME7828 YK1100 F- trpC9941 W3110 trpC9941 LB, 37C 29845, 30137, 3187, 3195, 3201, 3259, 3196 ME7829 SH6067 F- mukBΔ(XhoI-HpaI)::kan SH6066 W3110 TD./PI mukBΔ(XhoI-HpaI)::kan Kan (30 μg/ml) at 22C LB + Kan(30 μg/ml) at 22C ME7830 YM171 F- Other recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F' proAB lacIqZΔM15 Tn10 (Tet-R)] XL1 Blue pMY105 Amp resistant LB + Amp, 37C pMY105 30159 ME7831 YM198 = AZ5429 F- F' Other thi-1 gyrA96 recA1 relA1 lac supE44 hsdR17 endA1 XL1 Blue TF. pKX789 LB + Amp, 37C pKX789 carrying the His6 tagged mukF gene 30159 ME7832 YM289 F' trpA62 dadR [F' lacIq lacZDM15 proAB Tn10 (Tet-R)] tna-5 trpE61 PB103 CROSS F' lacIq lacZDM15 proAB Tn10 (Tet-R) LB + Tet, 37C 30159 ME7833 YM293 F' tna-5 trpE61 dadR [F' lacIq lacZDM15 proAB Tn10 (Tet-R)] trpA62 A33 F' lacIq lacZDM15 proAB Tn10 (Tet-R) LB + Tet, 22C Strain A33 is a mukF-disrupted strain, which was prepared by Dr. Aline Jaffe. The parental strain is PB103. 30159 ME7834 YM297 F' mukE::kan dadR trpE61 trpA62 tna-5 A34 = PB103 mukE:kan [F' lacIq lacZDM15 proAB Tn10 (Tet-R)] Tet resistant LB + Tet, 22C Strain A34 is a mukF-disrupted strain, which was prepared by Dr. Aline Jaffe. The parental strain is PB103. 30159 ME7835 YM301 F' ΔmukB::kan dadR trpE61 trpA62 tna-5 GC7528 [F' lacIq lacZDM15 proAB Tn10 (Tet-R)] LB + Tet, 22C GC7528 is a mukB-disrupted strain. The parental strain is PB103. ME7836 YM328 = BZ703 F' λ+ λDE3 thi-1 supE44 hsdR17 recA1 endA1 gyrA96 lac relA1 XL1-Blue λDE3 LB, 37C 30159 ME7837 YM385 = BZ662 λ+ λDE3 tna-5 trpA62 trpE61 dadR λDE3 PB103 λDE3 LB, 37C 30159 ME7838 YM448 Other λ+ λDE3 thi-1 supE44 relA1 lac λDE3 recA1 endA1 gyrA96 hsdR17 XL1-Blue pLysS LB + Cm, 37C 30159 ME7839 YM570 = A26 Other tna-5 trpA62 trpE61 dadR PB103 pAX850 LB + Cm, 37C pAX850 carrying the stmA-mukFEB operon 30159 ME7840 YM571 Other trpE61 dadR mukBΔ(XhoI-XhoI)::kan tna-5 trpA62 PB103 mukBΔ(XhoI-HpaI)::kan TF. pAX850 Cm-resistant LB + Cm, 37C pAX850 carrying the smtA-mukFEB operon 30159 ME7841 YM572 = OT5 hsr- araΔ139 Δ(ara leu)7697 ΔlacX74 galU galK hsm+ strA mukBΔ(XhoI-XhoI)::kan MC1061 mukB Δ(XhoI-XhoI)::kan LB, 22C ME7842 YM599 = BZ294 = BZ464 λ+ (λ) zcb::Tn10 his ΔtrpE5 mukB+ smb-131(λ) LB, 37C smb-131 = C-half trancated ams gene ME7843 YM600 = BZ215 F- λ+ (λ) smb-131 his ΔtrpE5 ΔmukB::kan (λ) LB, 22C ME7844 YM601 = KK124 = RK4788 F- metE70 zcb::Tn10 ΔompA Δbut non gyrA219 thi deoC flb-5301 rpsL150 recA1 araD139 Δ(argF-lac)U169 LB, 37C ME7845 YM621 = AZ5040 ΔmukB::kan tna-5 trpA62 trpE61 dadR smbA40 GC7528 smaA40 Grow at 42C (suppressor of the mukB null mutation) LB, 37C smaA is pyrH (UMP kinase) [Hiraga,2000, Annu. Rev. Genet. 34:21-59] 29790 ME7846 YM630 F' ΔmukB::kan dadR trpA62 trpE61 smbA40 tna-5 YM621 [F' proAB lacIqZΔM15 Tn10 (Tet-R)] Tet resistant LB + Tet, 37C ME7847 YM676 Other λ+ λDE3 (λDE3) trpA62 trpE61 dadR tna-5 PB103 λDE3 LB + Cm, 37C ME7848 YM678 F- λ+ λDE3 E. coli B F- dcm ompT hsdS(rB- mB-) gal (λDE3) BL21 (Stratagene) (λDE3) ME7849 YM685 Other mukBΔ(XhoI-XhoI)::kan araD139 Δ(ara leu)7697 ΔlacX74 galU galK hsr- hsm+ strA YM572 LB + Cm, 22C 30159 ME7850 YM687 Other YM572 LB + Cm, 22C 30159 ME7851 YM689 Other YM572 LB + Cm, 37C 30159 ME7852 YM691 Other YM572 LB + Cm, 22C 30159 ME7853 YM693 Other YM572 LB + Cm, 37C 30159 ME7854 YM695 Other YM572 LB + Cm, 37C 30159 ME7855 YM697 Other YM572 LB + Cm, 22C 30159 ME7856 YM699 Other YM572 LB + Cm, 22C 30159 ME7857 YM701 Other YM572 LB + Cm, 22C 30159 ME7858 YM707 Other YM572 LB + Cm, 22C 30159 ME7859 YM709 Other YM572 LB + Cm, 22C unpublished ME7860 YM720 = A21 Other PB103 LB + Cm, 37C ME7861 YM722 Other YM572 LB + Cm, 22C 30159 ME7862 YM724 Other YM572 LB + Cm, 37C 30159 ME7863 YM727 Other YM572 LB + Cm, 22C Grow slower at 37C than the wild-type strain. 30159 ME7864 YM732 λ+ λDE3 YM572 22C ME7865 YM747 Other λ+ λDE3 BL21 LB + Cm, 37C ME7866 YM749 Other λ+ λDE3 BL21 LB + Cm, 37C ME7867 YM758 = AR3076,GM124 lacZ118oc dam-4 rpsL275(Sm-R) 37C ME7868 YM879 = KK184 Other YK1100 LB + Amp, 37C pML6 is a plasmid which over expresses SeqA (from Dr. M. Kohiyama). ME7869 YM889 Other XL1-Blue LB + Amp, 37C vector expresses seqA-(His)6 ME7870 YM890 Other XL1-Blue LB + Amp, 37C vector expresses (His)6-seqA ME7871 YM897 Other λ+ λDE3 XL1-Blue LB + Amp, 37C vector expresses seqA-(His)6 ME7872 YM899 Other λ+ λDE3 XL1-Blue LB + Amp, 37C vector expresses (His)6-seqA ME7873 YM901 Other λ+ λDE3 XL1-Blue LB + Amp,Cm, 37C vector expresses seqA-(His)6 ME7874 YM903 Other λ+ λDE3 XL1-Blue LB + Amp,Cm, 37C vector expresses (His)6-seqA ME7875 YM908 Other XL1-Blue LB + Amp, 37C vector expresses FtsZ ME7876 YM914 Other λ+ λDE3 BL21 LB + Amp, 37C vector expresses FtsZ ME7877 YM944 Other XL1-Blue LB + Amp, 37C vector(pQE60) expresses MukB-His. ME7878 YM945 NC6100 from Dr. Gavin Recchia ME7879 YM948 supF6(Ts) metE KH5402-1(ilv thyA thr thr(Am) trpE9829(Am) deo KH5402-1 37C 29863, 3190 ME7880 YM949 F- ΔhobH KH5402-1 From M. Kohiyama 37C ME7881 YM952 Other W3110 LB + Amp, 37C from Elena (lac::aphA) ME7882 YM954 = KK144 F- ΔmukFEB PB103 22C ME7883 YM1002 Other λ+ λDE3 XL1-Blue LB + Amp, 37C mul operon expression vector ME7884 YM1022 = NK7254,KK133 F- his-1 argG str-104 tonA mtl-2 ΔlacZr1 leu-6 supE44 tsx trp-31 xyl-7 metB1 37C from Dr. Kreckner ME7885 YM1023 = NK7253,KK134 NK7254(seqA::tet) 37C from Dr. Kreckner ME7886 YM1024 λ+ λDE3 NK7254 37C ME7887 YM1026 λ+ λDE3 NK7253 37C ME7888 YM1028 = KS272 thi galE F-ΔlacX74 ΔphoA(PvuII) rpsL galK 37C from Dr. Beckwith ME7889 YM1029 = LMG194 KS272(Δara714,leu::Tn10) 37C from Dr. Beckwith ME7890 YM1030 F' OT7 22C ME7891 YM1032 λ+ λDE3 OT7 22C ME7892 YM1050 Other λ+ λDE3 BL21 LB + Amp, 37C Expression vector of B. subtilis SMC, form Dr. Erickson ME7893 YM1053 Other XL1-Blue LB + Amp, 37C from Dr. Yohichirou Itoh (Saitama University) ME7894 YM1054 = BZ690 λ+ λDE3 PB103(ΔmukF) A33 22C ME7895 YM1055 = B668 λ+ λDE3 PB103(ΔmukE) A34 22C ME7896 YM1056 = BZ666 F- λ+ λDE3 ΔmukB GC7528 22C PB103(DmukB) ME7897 YM1067 = KK7 PB103(ftsZ84) 30C ME7898 YM1068 = SH9077 zcb::Tn10 PB103 37C ME7899 YM1069 = SH9079 mukB106 zcb::Tn10 PB103 30C ME7900 YM1070 = SH9075 zcb::Tn10 mukB33 PB103 30C ME7901 YM1071 BL21 37C from Dr Yasuda (NIG) ME7902 YM1073 λ+ λDE3 ΔmukB::kan smbA40 AZ5040 LB, 37C ME7903 YM1080 Other XL1-Blue 37C vector expresses GST-BDTC(from Dr.Iwane at Osaka University, Dr. Yanagida's lab) ME7904 YM1099 = KH5402-1,KK137 thyA metE ilv thrA[amber] supF[Ts] deo trpE9829[amber] thr LB + Thymine, 37C ME7905 YM1100 = KA468,KK138 YM1099(dam-) 37C same as YM1099 except for dam13::Tn9[Spc-R] ME7906 YM1112 Other λ+ λDE3 BL21 LB + Cm, 37C another clone different from YM747 ME7907 YM1139 = KK143 dnaCts PC2 LB + thyminre (50 μg/ml), 30C ME7908 YM1140 = KK160 PC2 seqA::tet LB + thy 50, 30C ME7909 YM1143 F- PC2 (ΔmukB::km) LB + Thymine (50 μg/ml), 22C clone #1 ME7910 YM1144 PC2 (DmukB::km) LB + thy 50, 22C clone #2 ME7911 YM1145 PC2 (DmukB::km) LB + thy 50, 22C clone #3 ME7912 YM1146 PC2 (DmukB::km) LB + Thymine (50 μg/ml), 22C clone #4 ME7913 YM1147 PC2 (DmukB::km) LB + thy 50, 22C clone #5 ME7914 YM1149 = KK259 YK1100 seqA::tet 37C ME7915 YM1150 = ME8775 PC2 LB + thy 50, 22C from Dr. Nishimura, dnacC1 leu thi lac str ME7916 YM1160 thyA dnaCts PC2 LB + Thymine (50 μg/ml), 30C After single clone isolation of PC2(YM1150), tested for ts.
Usede in Sunako et al. (2001) Mol. Mircobiol. 42:1233-11241
66247 ME7917 YM1161 Other λ+ λDE3 BL21 LB + Kn, 37C Novagen control N ME7918 YM1164 = KK339 PC2 (dnaC+) 37C Transduction by P1vir phage grown on YK1100. Can grow at42C. ME7919 YM1165 = KK341 PC2 (dam-) 30C Transduction by P1vir phage grown on KK330. 3259 ME7920 YM1170 Other seqAΔ::tet YM1026 LB + Cm, 37C ME7921 YM1174 seqAΔ::tet PC2 30C 3259 ME7922 YM1179 Other λ+ λDE3 BL21 LB + Amp, 37C vector expresses smtA/mukF/mukE-His ME7923 YM1183 Other λ+ λDE3 BL21 LB + Amp, 37C vector expresses smtA/mukF/mukE ME7924 AZ5415 = SH9073 F- zcb::Tn10 trpC9941 YK1100 TD./PI ME7925 AZ5416 = SH9071 zcb::Tn10 trpC9941 mukB33 YK1100 TD./PI zcb::Tn10 mukB33 ME7926 AZ5417 = SH9083 F- mukB106 trpC9941 zcb::Tn10 YK1100 TD./PI zcb::Tn10 mukB106 ME7927 AZ5374 Other trpC9941 YK1100 pACY184 Cm 30137 ME7928 AZ5380 F- Other trpC9941 YK1100 TF. pKX769 Cm 30137 ME7929 KY1429 F- deoC1 flbB5301 thiA1 relA1 rpsL150 araD139 rbsR Δ(argF-lac)U169 zhf50::Tn10 rpoH6(Amber) ptsF25 MC4100 TD./PI rpoH6(Amber) zhf50::Tn10
Tetracycline resistance L broth at 30C Unable to grow at high temperature (42C) 30094, 29769, 29851, 30017 ME7930 KY1429[λpF13-(PrpoDhs-lacZ)] F- λ+ λpF13-(PrpoDhs-lacZ) araD139 Δ(argF-lac)U169 zhf50::Tn10 rpoH6(Amber) flbB5301 rbsR ptsF25 deoC1 relA1 thiA1 rpsL150 KY1429 OTHER METHODS Lysogenization Prophage λpF13-(PrpoDhs-lacZ) λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 30017, 29769, 29851, 30094 ME7931 KY1429 [λpF13-(PgroE-lacZ)] F- λ+ λpF13-(PgroE-lacZ) araD139 relA1 thiA1 rpsL150 Δ(argF-lac)U169 zhf50::Tn10 rpoH6(Amber) flbB5301 deoC1 ptsF25 rbsR KY1429 OTHER METHODS Lysogenization Prophage λpF13-(PgroE-lacZ) λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 30017, 29769, 29851, 30094 ME7932 KY1430 F- relA1 flbB5301 deoC1 ptsF25 rbsR Δ(argF-lac)U169 zhf50::Tn10 rpoH11(Ochre) thiA1 rpsL150 araD139 MC4100 TD./PI rpoH11(Ochre) zhf50::Tn10 Tetracycline resistance L broth at 30C Unable to grow at high temperature (42C) 30017, 29851, 29769, 30094 ME7933 KY1430[λpF13-(PrpoDhs-lacZ)] F- λ+ λpF13-(PrpoDhs-lacZ) rbsR ptsF25 deoC1 flbB5301 relA1 rpoH11(Ochre) zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 KY1430 OTHER METHODS Lysogenization Prophage λpF13-(PrpoDhs-lacZ) λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 29769, 30017, 29851, 30094 ME7934 KY1430 [(λpF13-(PgroE-lacZ)] F- λ+ λpF13-(PgroE-lacZ) flbB5301 rpoH11(Ochre) zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 deoC1 ptsF25 rbsR KY1430 OTHER METHODS Lysogenization Prophage λpF13-(PgroE-lacZ) λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 29851, 30094, 30017, 29769 ME7935 KY1431 F- rbsR rpoH15 zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 MC4100 TD./PI rpoH15 zhf50::Tn10 Tetracycline resistance L broth at 30C Unable to grow at high temperature (42C) 30017, 29851, 29769, 30094 ME7936 KY1431[λpF13-(PrpoDhs-lacZ)] F- λ+ λpF13-(PrpoDhs-lacZ) zhf50::Tn10 rbsR ptsF25 deoC1 flbB5301 relA1 thiA1 rpsL150 araD139 Δ(argF-lac)U169 rpoH15 KY1431 OTHER METHODS Lysogenization Prophage λpF13-(PrpoDhs-lacZ) λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 30017, 29851, 29769, 30094 ME7937 KY1431[λpF13-(PgroE-lacZ)] F- λ+ λpF13-(PgroE-lacZ) rbsR ptsF25 deoC1 flbB5301 relA1 thiA1 rpsL150 araD139 Δ(argF-lac)U169 zhf50::Tn10 rpoH15 KY1431 OTHER METHODS Lysogenization Prophage λpF13-(PgroE-lacZ) λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 29769, 29851, 30017, 30094 ME7938 KY1431 Tets F- rpsL150 rpoH15 Δ(argF-lac)U169 araD139 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR KY1431 PENICILLIN SPONTANEOUS Loss of Δzhf50::Tn10 Tetracycline sensitivity L broth at 30C Unable to grow at high temperature (42C) 29769, 30094, 30017, 29851 ME7939 KY1432 F- rbsR rpoH16(Amber) zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 MC4100 TD./PI rpoH16(Amber) zhf50::Tn10
Tetracycline resistance L broth at 30C Unable to grow at high temperature (42C) 30017, 29769, 29851, 30094 ME7940 KY1432[λpF13-(PgroE-lacZ)] F- λ+ λpF13-(PgroE-lacZ) rbsR rpoH16(Amber) zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 KY1432 OTHER METHODS Lysogenization Prophage λpF13-(PgroE-lacZ)] λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 29769, 30094, 30017, 29851 ME7941 KY1433 F- relA1 rpoH18(Amber) zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 flbB5301 deoC1 ptsF25 rbsR MC4100 TD./PI rpoH18(Am) zhf50::Tn10
Tetracycline resistance L broth at 30C Unable to grow at high temperature (42C) 30094, 30017, 29851, 29769 ME7942 KY1433[λpF13-(PrpoDhs-lacZ)] F- λ+ λpF13-(PrpoDhs-lacZ) rpoH18(Amber) rbsR ptsF25 deoC1 flbB5301 relA1 thiA1 rpsL150 araD139 Δ(argF-lac)U169 zhf50::Tn10 KY1433 OTHER METHODS Lysogenization Prophage λpF13-(PrpoDhs-lacZ) λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 30094, 29769, 29851, 30017 ME7943 KY1433[λpF13-(PgroE-lacZ)] F- λ+ λpF13-(PgroE-lacZ) rbsR ptsF25 deoC1 flbB5301 relA1 thiA1 rpsL150 araD139 Δ(argF-lac)U169 zhf50::Tn10 rpoH18(Amber) KY1433 OTHER METHODS Lysogenization Prophage λpF13-(PgroE-lacZ) λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 30017, 29851, 29769, 30094 ME7944 KY1434 F- deoC1 rpoH22(Amber) zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 ptsF25 rbsR MC4100 TD./PI rpoH22(Amber) zhf50::Tn10
Tetracycline resistance L broth at 30C Unable to grow at high temperature (42C) 29769, 30094, 30017, 29851 ME7945 MC4100 rpoH165 F- λ+ rbsR rpoH165(Amber) zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 MC4100 TD./PI rpoH165(Amber) zhf50::Tn10 Tetracycline resistance L broth at 30C Unable to grow at high temperature (42C) 30094 ME7946 MC4100 rpoH165 [λpF13-(PrpoDhs-lacZ)] F- λ+ λpF13-(PrpoDhs-lacZ) zhf50::Tn10 ptsF25 rbsR deoC1 flbB5301 relA1 thiA1 rpsL150 araD139 Δ(argF-lac)U169 rpoH165(Amber) MC4100 OTHER METHODS Lysogenization Prophage λpF13-(PrpoDhs-lacZ) λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 30017, 29769, 30094, 29851 ME7947 MC4100 rpoH165 [λpF13-(PgroE-lacZ)] F- λ+ λpF13-(PgroE-lacZ) araD139 thiA1 relA1 flbB5301 rpoH165(Amber) zhf50::Tn10 Δ(argF-lac)U169 rbsR ptsF25 deoC1 rpsL150 MC4100 OTHER METHODS Lysogenization Prophage λpF13-(PgroE-lacZ) λ phage immunity L broth at 30C Unable to grow at high temperature (42C) 30094, 29851, 29769, 30017 ME7948 KY1601; MC4100 DrpoH F- λ+ λpF13-(PrpoDhs-lacZ) ptsF25 deoC1 flbB5301 relA1 araD139 thiA1 rpsL150 rbsR ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 MC4100 [λpF13-(PrpoDhs-lacZ)] TD./PI ΔrpoH30::kan zhf50::Tn10 Tetracycline resistance L broth at 20C Unable to grow at or above 30C 29819, 29817 ME7949 KY1612; MC4100 DrpoH::kan F- λ+ λpF13-(PgroE-lacZ) rpsL150 ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 araD139 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR MC4100 [λpF13-(PgroE-lacZ)] TD./PI ΔrpoH::kan zhf50::Tn10 Tetracycline resistance L broth at 20C Unable to grow at or above 30C 29819, 29817 ME7950 MC4100 DrpoH::cat F- araD139 Δ(argF-lac)U169 DrpoH::cat rbsR ptsF25 deoC1 flbB5301 relA1 thiA1 rpsL150 MC4100 TD./PI ΔrpoH::cat zhf50::Tn10 Chloramphenicol resistance L broth at 20C Unable to grow at or above 30C 29817, 29819 ME7951 MG1655 DrpoH F- λ+ zhf50::Tn10 ΔrpoH30::kan MG1655 TD./PI ΔrpoH30::kan zhf50::Tn10 Tetracycline resistance L broth at 20C Unable to grow at or above 30C 29817, 29819 ME7952 CAG9301 F- λ+ rpoH120::kan zhf50::Tn10 MG1655 TD./PI rpoH120::kan zhg-21::Tn10 Tetracycline resistance Carol Gross L broth at 20C Unable to grow at or above 30C 29817 ME7953 W3110 DrpoH::kan F- λ+ ΔrpoH30::kan zhf50::Tn10 W3110 TD./PI ΔrpoH30::kan zhf50::Tn10 Tetracycline resistance L broth at 20C Unable to grow at or above 30C 29817, 29819 ME7954 KY1612 pgroE F- Other λ+ λpF13-(PgroE-lacZ) ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR KY1612 TF. Plasmid pOF12 Ampicillin resistance L broth at 30C Unable to grow at 42C 29819 ME7955 KY1615; R30-1 F- λ+ λpF13-(PrpoDhs-lacZ) suhX301 ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR KY1601 SPONTANEOUS suhX301 Growth at higher temperature (30C) on L agar L broth at 30C Unable to grow at or above 37C 29819 ME7956 R30-2 F- λ+ λpF13-(PrpoDhs-lacZ) relA1 suhX302 ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 flbB5301 deoC1 ptsF25 rbsR KY1601 SPONTANEOUS suhX302 Growth at higher temperature (30C) on L agar L broth at 30C Unable to grow at or above 37C 29819 ME7957 R30-3 F- λ+ λpF13-(PrpoDhs-lacZ) zhf50::Tn10 rbsR ptsF25 deoC1 rpsL150 thiA1 relA1 suhX303 ΔrpoH30::kan araD139 flbB5301 Δ(argF-lac)U169 KY1601 SPONTANEOUS suhX303 Growth at higher temperature (30C) on L agar L broth at 30C Unable to grow at or above 37C 29819 ME7958 KY1603; R40-1 F- λ+ λpF13-(PrpoDhs-lacZ) ptsF25 relA1 thiA1 rpsL150 araD139 Δ(argF-lac)U169 zhf50::Tn10 ΔrpoH30::kan suhX401 flbB5301 rbsR deoC1 KY1601 SPONTANEOUS suhX401 Growth at higher temperature (40C) on L agar L broth at 30-40C Unable to grow at or above 42C or at 20C or below 29819 ME7959 KY1606; R40-2 F- λ+ λpF13-(PrpoDhs-lacZ) rbsR relA1 suhX402 thiA1 rpsL150 araD139 Δ(argF-lac)U169 zhf50::Tn10 ΔrpoH30::kan flbB5301 deoC1 ptsF25 KY1601 SPONTANEOUS suhX402 Growth at higher temperature (40C) on L agar L broth at 30-40C Unable to grow at or above 42C or at 20C or below 29819 ME7960 KY1607; R40-3 F- λ+ λpF13-(PrpoDhs-lacZ) ptsF25 suhX403 ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 rbsR KY1601 SPONTANEOUS suhX403 Growth at higher temperature (40C) on L agar L broth at 30-40C Unable to grow at or above 42C or at 20C or below 29819 ME7961 KY1617; R42-1 F- λ+ λpF13-(PgroE-lacZ) rbsR suhX302 suhY421 ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 KY1612; R30-2 SPONTANEOUS suhY421 Growth at higher temperature (42C) on L agar L broth at 37C 29819 ME7962 KY1618; R42-2 F- λ+ λpF13-(PgroE-lacZ) ptsF25 suhX302 suhY422 ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 rbsR KY1612; R30-2 SPONTANEOUS suhY422 Growth at higher temperature (42C) on L agar L broth at 37C 29819 ME7963 NRK117 F- ptsF25 groEL44 zje::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 rbsR MC4100 TD./PI groEL44 zje::Tn10 Tetracycline resistance L broth at 30C Unable to grow at high temperature (42C) 29806 ME7964 NRK156 F- dnaK756 rbsR ptsF25 deoC1 flbB5301 relA1 thiA1 rpsL150 araD139 Δ(argF-lac)U169 thr34::Tn10 MC4100 TD./PI dnaK756 thr34::Tn10 Tetracycline resistance N. Kusukawa L broth at 30C Unable to grow at high temperature (42C) 29801, 30057 ME7965 KY1880 F- Other rbsR ΔgroE68::tet thiA1 rpsL150 araD139 Δ(argF-lac)U169 relA1 flbB5301 deoC1 ptsF25 MC4100 pKV1561 TD./PI ΔgroE68::tet Tetracycline resistance L broth containing 50mM IPTG at37C Requires IPTG for growth particularly at high temperature (42C) 30121 ME7966 KY1156 F- λ+ λCharon25-lacUV5p-groESgroEL ptsF25 ΔgroE68::tet Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 rbsR KY1880 MC4100 (λCharon25-lacUV5p-groESgroEL) TD./PI ΔgroE68::tet Tetracycline resistance L broth containing 1mM IPTG at 37C Requires IPTG for maximum growth 30171 ME7967 KY2039 F- deoC1 rbsR ptsF25 flbB5301 ΔhslVU1172::tet Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 MC4100 ΔhslVU1172::tet Tetracycline resistance L broth at 37C 30155 ME7968 KY2263 F- thiA1 araD139 flbB5301 relA1 rpsL150 rbsR ptsF25 deoC1 Δ(argF-lac)U169 Δ(clpPX-lon)1196::cat MC4100 Δ(clpPX-lon)1196::cat Chloramphenicol resistance L broth at 37C Slow growth at or above 42C 30147 ME7969 KY2347 F- Δ(clpPX-lon)1196::cat MG1655 TD./PI Δ(clpPX-lon)1196::cat Chloramphenicol resistance L broth at 37C Slow growth below 37C 30154 ME7970 KY2966 F- ΔhslVU1172::tet MG1655 ΔhslVU1172::tet Tetracycline resistance L broth at 37C 30154, 35616 ME7971 MC4100[λpF13-(PrpoDhs-lacZ)] F- λ+ λpF13-(PrpoDhs-lacZ); Transcriptional (operon) fusion rpsL150 Δ(argF-lac)U169 araD139 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR MC4100 OTHER METHODS Lysogenization Prophage λpF13-(PrpoDhs-lacZ) λ phage immunity L broth at 30C λ prophage reporter for transcription from the rpoDhs heat shock promoter 30017, 30094, 29851, 29769 ME7972 MC4100[λpF13-(PgroE-lacZ)] F- λ+ λpF13-(PgroE-lacZ); transcriptional (operon) fusion deoC1 thiA1 relA1 flbB5301 ptsF25 rbsR Δ(argF-lac)U169 araD139 rpsL150 MC4100 OTHER METHODS Lysogenization Prophage λpF13-(PgroE-lacZ) L phage immunity L broth at 30C λ prophage reporter for transcription from the groE heat shock promoter 29769, 29851, 30094, 30017 ME7973 MC4100 (λOF-0) F- λ+ λOF-0 carrying an operon fusion PrpoH-lacZ Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 rbsR flbB5301 deoC1 ptsF25 MC4100 OTHER METHODS Lysogenization Prophage λOF-0 carrying an operon fusion PrpoH-lacZ L broth at 37C λ prophage reporter for transcription from the rpoH promoters 29795 ME7974 MC4100(λGF807) F- λ+ λGF807 carrying a translational (gene) fusion rpoH-lacZ relA1 rbsR thiA1 rpsL150 araD139 Δ(argF-lac)U169 ptsF25 deoC1 flbB5301 MC4100 OTHER METHODS Lysogenization Prophage λGF807 carrying gene fusion rpoH(base 1-807)-lacZ L broth at 37C λ prophage reporter for translation of rpoH (the resulting fusion protein is quite unstable) 29795 ME7975 MC4100 (λGF364) F- λ+ λGF364 carrying a translational (gene) fusion [rpoH(1-364)-lacZ] thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR Δ(argF-lac)U169 araD139 rpsL150 MC4100 OTHER METHODS Lysogenization Prophage λGF364 carrying a gene fusion rpoH(base 1-364)-lacZ L broth at 37C λ prophage reporter for translation of rpoH (the resulting fusion protein is stable) 29795 ME7976 MC4100 pKV1 F- Other araD139 Δ(argF-lac)U169 rbsR flbB5301 deoC1 ptsF25 relA1 thiA1 rpsL150 MC4100 TF. Plasmid pKV1 Ampicillin resistance L broth plus ampicillin at 37C 30017, 29769, 29851, 30094 ME7977 MC4100 pKV3 F- Other ptsF25 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 rbsR MC4100 TF. Plasmid pKV3
Ampicillin resistance L broth plus ampicillin at 37C 30094, 30017, 29851, 29769 ME7978 MC4100 pKV6 F- Other rbsR deoC1 flbB5301 relA1 thiA1 Δ(argF-lac)U169 araD139 rpsL150 ptsF25 MC4100 TF. Plasmid pKV6 [pKV2 carrying the rpoH165(Amber) mutation]
Ampicillin resistance L broth plus ampicillin at 37C 30094 ME7979 MC4100 pKV7 F- Other ptsF25 deoC1 flbB5301 relA1 thiA1 Δ(argF-lac)U169 rpsL150 araD139 rbsR MC4100 TF. Plasmid pKV7 Ampicillin resistance L broth plus ampicillin at 37C 29798 ME7980 MC4100 pKV10 F- Other flbB5301 thiA1 rpsL150 araD139 Δ(argF-lac)U169 rbsR ptsF25 deoC1 relA1 MC4100 TF. Plasmid pKV10 Ampicillin resistance L broth plus ampicillin at 37C 30094 ME7981 HI1006 pKV1561 F- Other galK galU Δ(lacIPOZY)x74 Δ(araABIOC-Leu)7697 araD139 HI1006 TF. Plasmid pKV1561 Ampicillin resistance L broth plus ampicillin at 30C Expression plasmid for GroES and GroEL 30121 ME7982 JM109 pDsbCD1 F- Other JM109 TF. pDsbCD1 Chloramphenicol resistance L broth plus chloramphenicol at 37C Expression plasmid for DsbCD 30163, 3327, 3313 ME7983 JM109 pDsbABCD1 F- Other JM109 TF. pDsbABCD1 Chloramphenicol resistance L broth plus chloramphenicol at 37C Expression plasmid for DsbABCD 30163 ME7984 MC4100 F- ptsF25 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 rbsR Koreaki Ito L broth at 37C Parental strain of most heat shock-related srains (Yura lab.) 29851, 66199, 30094, 30017, 29769 ME7985 W3110 F- A. Matsushiro L broth at 37C E. coli strain K12 prototroph 35427, 35437, 35491 ME7986 MG1655 F- B. Bachmann L broth at 37C "E. coli strain K12 prototroph
sequence (FASTQ format)
https://ddbj.nig.ac.jp/search/entry/sra-run/DRR058060" 66189, 4979, 4969, 59197, 35668, 56351, 35355, 4953, 4958, 4978, 4983, 35563, 35599, 35609, 4974, 3765, 4985, 35660, 4965, 4966 ME7987 MG1655 D(argF-lac)U169 F- zah::Tn10 Δ(argF-lac)U169 MG1655 TD./PI Δ(argF-lac)U169 zah::Tn10 Tetracycline resistance L broth at 37C Host for lacZ-reporter constructs ME7988 W3110 D(argF-lac)U169 F- Δ(argF-lac)U169 zah::Tn10 W3110 TD./PI Δ(argF-lac)U169 zah::Tn10 Tetracycline resistance L broth at 37C Host for lacZ-reporter constructs ME5305 AG1 F- relA1 recA1 endA1 gyrA96 thi-1 hsdR17(rk- mk+) supE44 Add 1 µg/ml of thiamine and 40 µg/ml of histidine into the medium, when you use minimal medium. "AG1 has uncharacterized mutations including mutation that improves transformation efficiency and mutation that causes auxotrophy for histidine.
sequence (FASTQ format)
https://ddbj.nig.ac.jp/search/entry/sra-run/DRR058069" 35654, 4963, 3294, 4962, 4913, 53793, 55829, 55816, 35186, 35367, 35289, 35555 ME5306 W3110 F- IN(rrnD-rrnE)1 A. Matsushiro -> T. Yura -> R. Okazaki -> Y. Kohara Sigma type B ME9062 BW25113 rph-1 (old genotype: lac1q rrnBT14 DlacZWJ16 hsdR514 DaraBADAH33 DrhaBADLD78) rrnB DElacZ4787 HsdR514 DE(araBAD)567 DE(rhaBAD)568 "sequence (FASTQ format)
https://ddbj.nig.ac.jp/search/entry/sra-run/DRR058068" 74401, 74402, 74373, 74375, 74376, 74379, 74372, 66195, 66236, 84953, 84956, 84950, 84948, 84952, 78976, 78977, 78984, 79175, 74371, 84958 IH1 C600S F- tonB+(φ80s) supE lac- thi- HfrH C600 mating φ80S Thr+ Leu+ Osaka University (A. Matushiro) ordinary conditions(LB 37degreeC) C600S is sensitive strain against φ80, although the parental strain C600 is resistant. IH2 BT13 F- supD lac1000 trpam metam3 strr bfeam tsxam CR63 BT3 P1-transduction Su+amber Trpam+ Kyoto University (F. Yamao) ordinary conditions(LB 37degreeC) IH3 BT22 F- bfeam tsxam supE lac1000 trpam strr C600 BT52 P1-transduction Su+amber Trpam+ Kyoto University (F. Yamao) ordinary conditions(LB 37degreeC) IH4 BT32 F- bfeam supF lac1000 trpam strr tsxam Ymel BT52 P1-transduction Su+amber Trpam+ Kyoto University (F. Yamao) ordinary conditions(LB 37degreeC) IH5 BT63 F- supP tsxam bfeam strr metam3 trpam lac1000 2B6 BT3 P1-transduction Su+amber Trpam+ Kyoto University (F. Yamao) ordinary conditions(LB 37degreeC) IH6 CAJ64 F+(?) SupUGA Su+UGA Cambridge University (S. Brenner) ordinary conditions(LB 37degreeC) A classical strain carrying an UGA suppressor. The mutation is a change of G24->A24 in the DHU-stem of Trp tRNA. IH7 BT52 F- su0 lac1000 strr bfeam tsxam trpam 1000BT spontaneous mutation bfeam, tsxam ordinary conditions(LB 37degreeC) Two amber mutations in the receptor for both phages of BF23 and T6. IH8 BT3 F- trpam bfeam metam3 su0 lac1000 tsxam strr BT52 spontaneous mutation metam Kyoto University (F. Yamao) ordinary conditions(LB 37degreeC) The suppression of lacZam and metam are specific for Su+ amber. IH9 BT235 F- trpam cysam235 tsxam bfeam strr lac1000 su0 BT52 spontaneous mutation cysam Kyoto University (F. Yamao) ordinary conditions(LB 37degreeC) The suppression of lacZam and cysam are specific for Su+ amber. IH10 CA274 F- tsx- strr su0 thr- leu- proA- his- argE- lacY- galK- ara- xyl- mlt- spontaneous mutation Osaka University (S. Kondoh) ordinary conditions(LB 37degreeC) The strain carrying double mutations of amber. IH11 AB1157Lac- F- strr mlt- tsx- lacZoc thr- leu- proA- his- argE- galK- ara- xyl- CA293 marker exchange with transducing phage lacZoc659 ordinary conditions(LB 37degreeC) The strain carrying triple mutations of ochre. IH12 9829 F- trpam tyram W3110 trpam spontaneous mutation tyram Kyoto University (T. Nagata) ordinary conditions(LB 37degreeC) Double markers for amino acid requirment are amber mutation. IH13 KY1340 F- suO recA tyram met- trpam his- arg- 9829 F'recA--duction uv-sensetivity thyA+ Kyoto University (T. Nagata) ordinary conditions(LB + thy 37degreeC) A recA- strain drived from the strain 9829. IH14 AB1157 F- trpAam9605 rifR lacZUGA659 leuUGA hisam29 ilv- thyA- metB- argH- strr lacZUGA、leuUGA Kyoto University (H. Ozeki) ordinary conditions(LB 37degreeC) The strain carrying lacZUGA and leuUGA, and also amber mutations. IH15 C600S/λ F- supE malT thi- lac- tonB+(φ80s) C600S spontaneous mutation λR Kyoto University ordinary conditions(LB 37degreeC) IH16 C600S/λ(80) F- Other φ80 malT lac- thi- tonB+(φ80s) supE C600S/λ lysogenization φ80 immunity Kyoto University ordinary conditions(LB 37degreeC) IH17 CAJ64/λ F(?) malT SupUGA CAJ64 spontaneous mutation λR Kyoto University ordinary conditions(LB 37degreeC) The strain carrying UGA suppressor IH18 CAJ64/80 F(?) tonB(φ80r) SupUGA CAJ64 spontaneous mutation φ80R Kyoto University ordinary conditions(LB 37degreeC) The strain carrying UGA suppressor IH19 LE392/λ F- hsdR514 supF58 supE44 malT lacY1 metB1 galT22 trpR55 galK2 LE392 spontaneous mutation λR Kyoto University ordinary conditions(LB 37degreeC) This strain behave two amber suppressors, supE and supF. IH20 LE392/80 F- hsdR514 tonB(φ80r) supE44 supF58 galK2 galT22 metB1 trpR55 lacY1 LE392 spontaneous mutation φ80R Kyoto University ordinary conditions(LB 37degreeC) This strain behave two amber suppressors, supE and supF. IH21 LE392(λgtc) F- Other λ tonB(φ80r) lacY1 trpR55 metB1 galT22 galK2 hsdR514 supF58 supE44 LE392/80 lysogenization λimmunity Kyoto University ordinary conditions(LB 30degreeC) This strain behave two amber suppressors, supE and supF. IH22 LE392 (φ80sus2) F- Other φ80sus2 trpR55 metB1 galT22 galK2 hsdR514 supF58 supE44 lacY1 LE392 lysogenization φ80 immunity Kyoto University ordinary conditions(LB 37degreeC) This strain behave two amber suppressors, supE and supF. IH23 LE392 (λimm21) F- Other λimm21 lacY1 supE44 supF58 hsdR514 galK2 galT22 metB1 trpR55 LE392 lysogenization φ21 immunity Kyoto University ordinary conditions(LB 37degreeC) This strain behave two amber suppressors, supE and supF. IH24 LE392 (λimm21nin5) F- Other λimm21nin5 metB1 galT22 galK2 hsdR514 supF58 supE44 lacY1 trpR55 LE392 lysogenization φ21 immunity Kyoto University ordinary conditions(LB 37degreeC) This strain behave two amber suppressors, supE and supF. IH25 C600 (λUam413) F- Other λUam413 tonA supE44 hsdR thr- leu- thi- lacY C600 lysogenization λ immunity Kyoto University ordinary conditions(LB 37degreeC) IH26 C600 (λKam24) F- Other λKam24 leu- tonA lacY thi- hsdR supE44 thr- C600 lysogenization λ immunity Kyoto University ordinary conditions(LB 37degreeC) IH27 594 (λLam63) F- Other λLam63 strr galT galK 594 lysogenization λ immunity Kyoto University ordinary conditions(LB 37degreeC) A defective lysate (head donor) able to prepare by prophage induction. IH28 C600 (λimm21) F- Other λimm21 lacY thr- supE46 leu- tonA hsdR thi- C600 lysogenization φ21 immunity Kyoto University ordinary conditions(LB 37degreeC) IH29 C600 (λimm434) F- Other λimm434 thr- tonA lacY thi- hsdR leu- supE46 C600 lysogenization φ434 immunity Kyoto University ordinary conditions(LB 37degreeC) IH30 CR63 (λimm21nin5h) F- Other λimm21nin5 supD prototroph λR CR63 lysogenization φ21 immunity Kyoto University ordinary conditions(LB + thy 37degreeC) h indicates host-range marker of phage λ; CR63 is resistant for the wild type λ. IH31 W3350 thyA- F- Other λcI857Sam7 galT galK thyA- W3350 TMP selection, lysogenization thy- λts-immunity Kyoto University ordinary conditions(LB 37degreeC) Useful for the prepareation of phage λ in large scale. IH32 TS709 F+(?) Other λ tsxam lacam su0 rnpB bfeam 4273 NTG temperature sensitivity Kyoto University ordinary conditions(LB 30degreeC) A mutant strain of tRNA processing; ts character is able to see more clearly at over 42degreeC and on a poor neutrient plate. IH33 W3110ΔssrA F- ssrA W3110 in vitro & P1(*) KmR ordinary conditions(LB 37degreeC) A disruptant of the gene for tmRNA (ssrA): (*)in viro nene manupulation -> transformation to JC7623 -> P1 transduction. IH34 TS15 F- Other λ prs W3110ΔssrA NTG & PS(*) temperature sensitivity ordinary conditions(LB 30degreeC) A ts mutant related the gene for tmRNA (ssrA) phenotypically; (*)Mutagenesis by NTG and penicillin-screening. IH35 TS101 F- Other λ prs W3110ΔssrA NTG & PS(*) temperature sensitivity ordinary conditions(LB 30degreeC) A ts mutant related the gene for tmRNA (ssrA) phenotypically; (*)Mutagenesis by NTG and penicillin-screening. IH36 LE392 prs ts101 F- galT22 galK2 prs supF58 HsdR514 lacY1 trpR55 metB1 supE44 LE392 P1-transduction temperature sensitivity ordinary conditions(LB 30degreeC) IH37 LE392ΔssrA prs ts101 F- galK2 supE44 supF58 HsdR514 prs ΔssrA galT22 metB1 trpR55 lacY1 LE392ΔssrA P1-transduction temperature sensitivity ordinary conditions(LB 30degreeC) A ts mutant related the gene for tmRNA (ssrA) phenotypically. IH38 W3110ΔsmpB ΔssrA F- ssrA smpB W3110 in vitro & P1(*) CmR Hirosaki University (A. Muto) ordinary conditions(LB 37degreeC) (*)Mutagenesis by NTG and penicillin-screening. IH39 TS2 F- Other λ hisS ΔssrA W3110ΔssrA NTG & PS(*) temperature sensitivity ordinary conditions(LB 30degreeC) A ts mutant related the gene for tmRNA (ssrA) phenotypically; (*)Mutagenesis by NTG and penicillin-screening. IH40 TS9 F- Other λ ΔssrA rho W3110ΔssrA NTG & PS(*) temperature sensitivity ordinary conditions(LB 30degreeC) A ts mutant related the gene for tmRNA (ssrA) phenotypically; (*)Mutagenesis by NTG and penicillin-screening. IH41 TS20 F- Other λ ΔssrA htrA W3110ΔssrA NTG & PS(*) temperature sensitivity ordinary conditions(LB 30degreeC) A ts mutant related the gene for tmRNA (ssrA) phenotypically; (*)Mutagenesis by NTG and penicillin-screening. IH42 W3110ΔssrA thyAts12-20 F- thyAts12-20 ΔssrA W3110ΔssrA NTG & PS(*) temperature sensitivity TMPR ordinary conditions(LB + thy 30degreeC) A ts mutant related the gene for tmRNA (ssrA) phenotypically; (*)Mutagenesis by NTG and penicillin-screening. IH43 TS2ΔthyA F- hisS ΔssrA ΔthyA TS2 in vitro & P1(*) thy CmR ordinary conditions(LB + thy + Cm 30degreeC) (*)Mutagenesis by NTG and penicillin-screening. IH44 WΔΔts2 (λthyAts12-20) F- Other λthyA hisS ΔssrA thyAts12-20 ΔthyA TS2ΔthyA lysogenization λimmunity, thyAts ordinary conditions(LB + thy 30degreeC) Double ts mutant related the gene for tmRNA (ssrA) phenotypically: behaving two drug-resistance markers, Cmr and Kmr IH45 KY2343 F- divE42 endA1100 recA1 thr metE rpoB tsx serC13 temperature sensitivity National Institute for Cancer Research (M. Ohki) ordinary conditions(LB 30degreeC) divE = a gene for tRNA Ser1. The phenotype of this mutant strain is temperature-sensitive. IH46 KY2343 TR4 F- rpoB metE thr recA1 divE ts-resistant suppressor endA1100 divE42 serC13 tsx KY2343 MUTAGENESIS/NTG ts revertant ordinary conditions(LB 37degreeC) A suppressor mutant of divE ts; it shows cold-sensitive phenotype. IH47 W3110ΔtRNALeu6 F- leuX W3110 in vitro & P1(*) KmR ordinary conditions(LB 30degreeC) (*)in viro nene manupulation -> transformation to JC7623 -> P1 transduction. IH48 W3110ΔmiaA F- miaA W3110 in vitro & P1(*) CmR ordinary conditions(LB 30degreeC) (*)in viro nene manupulation -> transformation to JC7623 -> P1 transduction. IH49 W3110ΔtRNALeu6ΔmiaA F- leuX miaA W3110ΔtRNALeu6 P1-transduction KmR, CmR ordinary conditions(LB 30degreeC) IH50 BT63 ts39 F- Other λ tsxam supP trpam metam3 strr bfeam lac1000 gidA BT63 NTG & PS(*) ts39 ordinary conditions(LB 30degreeC) (*)NTG-mutagenesis and penicillin-screening. IH51 W3110ΔtRNALeu6gidA* F- leuX gidA W3110ΔtRNALeu6 P1-transduction ts39 ordinary conditions(LB 30degreeC) IH52 BT63 ts29 F- Other λ trpam miaA lac1000 metam3 strr bfeam tsxam supP BT63 NTG & PS(*) ts29 ordinary conditions(LB 30degreeC) (*)NTG-mutagenesis and penicillin-screening. IH53 F1 F- thi supE Δ(lac-proAB) JM101 segregation of F' factor pro- ordinary conditions(LB 37degreeC) Usefull for a deletion strain of lacZ carrying Su+ suppressor supE. IH54 F1ΔthyA F- supE ΔthyA Δ(lac-proAB) thi TS2ΔthyA F1 P1-transduction thy- CmR ordinary conditions(LB + Cm 37degreeC) IH55 F1ΔssrAΔthyA F- ΔthyA thi ΔssrA Δ(lac-proAB) supE TS2ΔthyA F1ΔssrA P1-transduction thy- CmR ordinary conditions(LB + Cm 37degreeC) IH56 F1ΔssrA F- ΔssrA thi supE Δ(lac-proAB) W3110ΔssrA F1 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH57 9829ΔssrA F- tyram ΔssrA trpam W3110ΔssrA 9829 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH58 BT52ΔssrA F- bfeam strr trpam lac1000 su0 ΔssrA tsxam W3110ΔssrA BT52 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH59 C600ΔssrA F- lacY leu- thi- hsdR thr- supE46 ΔssrA tonA W3110ΔssrA C600 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH60 LE392ΔssrA F- metB1 supE44 ΔssrA supF58 lacY1 trpR55 HsdR514 galK2 galT22 W3110ΔssrA LE392 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH61 CA274ΔssrA F+(?) ΔssrA trpam lacam125 suO HfrC W3110ΔssrA CA274 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH62 BT13ΔssrA F- metam3 trpam ΔssrA lac1000 tsxam supD bfeam strr W3110ΔssrA BT13 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH63 BT22ΔssrA F- lac1000 ΔssrA trpam strr bfeam tsxam supE W3110ΔssrA BT22 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH64 BT32ΔssrA F- ΔssrA supF strr bfeam tsxam trpam lac1000 W3110ΔssrA BT32 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH65 BT3ΔssrA F- bfeam strr tsxam ΔssrA su0 lac1000 trpam metam3 W3110ΔssrA BT3 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH66 CR63ΔssrA F+(?) ΔssrA supD λR W3110ΔssrA CR63 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH67 BT63ΔssrA F- ΔssrA supP tsxam bfeam strr metam8 trpam lac1000 W3110ΔssrA BT63 P1-transduction ΔssrA KmR ordinary conditions(LB + Km 37degreeC) IH68 VS101 F+ HfrC suO hemH lacam125 trpam CA274 NTG & PS(*) Light-sensitive (visA) dark condition LB 37degreeC (*)With NTG-mutagenesis and penicillin-screening IH71 VS200 F+ lacam125 ΔvisA(=ΔhemH) HfrC suO trpam CA274 marker exchange with transducing phage ΔvisA LB + 0.2 % glucose, dark condition A deletion mutant of hemH (not substitution with drug-resistance marker). IH72 VS281 F+ suO hemP* ΔvisA(=ΔhemH) lacam125 trpam HfrC VS200 spontaneous mutation hemP* LB + 10μg/ml hemin, dark condition 37degreeC A hemin permeable mutant: high frequency of reversion. IH73 VS550 F+ lacam125 visB trpam HfrC suO CA274 NTG & PS(*) Light-sensitive (visB) dark condition LB A mutant of ubiH. (*)NTG-mutagenesis and penicillin-screening. IH74 H103 F+ hemA, trpam suO HfrC lacam125 visA(=ΔhemH) hemK VS200 spontaneous mutation light-resistant mutat of ΔvisA LB + 0.2 % glucose A double mutant of hemA and hemK IH75 VSR751 F+ lacam125 hemG visA(=ΔhemH) HfrC su0 trpam VS200 spontaneous mutation light-resistant mutat of ΔvisA LB + 0.2 % glucose, dark condition A point mutant of hemG IH76 VSR800 F+ Other λxxg1 HfrC lacam125 visA(=ΔhemH) hemG trpam su0 VSR751 lysogenization hemG+ LB + 0.2 % glucose, dark condition The strain carrying a transducing phage of hemG+ IH77 N500 F- lacY1 hemH hemA supE44 galT22 supF58 trpR55 metB1 HsdR514 galK2 LE392ΔhemA marker exchange with transducing phage ΔhemH LB + 0.2 % glucose + 50ug/ml ALA, dark condition Light-sensitive with addition of 5-aminolevlinic acid (ALA) IH78 N503 F- galT22 metB1 trpR55 HsdR514 supF58 supE44 lacY1 galK2 hemP* N500 spontaneous mutation hemP* LB + 10 μg/ml hemin, dark condition A hemin-permeable mutant: high frequency of reversion. IH79 W3110 hemG F- hemG LG285 W3110 P1-transduction hemG::KmR LB + 0.2 % glucose IH80 W3110 hemB F- hemB JC7623hemB W3110 in vitro & P1(*) hemB::KmR LB + 0.2 % glucose (*)in vitro gene manipulation->transformation to the strain JC7623->P1 transduction. IH81 W3110 hemE F- hemE JC7624hemE W3110 in vitro & P1(*) hemE::CmR LB + 0.2 % glucose (*)in vitro gene manipulation->transformation to the strain JC7623->P1 transduction. IH82 W3110 cysG F- cysG JC7625cysG W3110 in vitro & P1(*) cysG::KmR LB + 0.2 % glucose (*)in vitro gene manipulation->transformation to the strain JC7623->P1 transduction. IH83 W3110 hemL F- hemL JC7626hemL W3110 in vitro & P1(*) hemL::SpcR LB + 0.2 % glucose (*)in vitro gene manipulation->transformation to the strain JC7623->P1 transduction. IH84 W3110 hemA F- hemA LE392ΔhemA W3110 P1-transduction hemA::KmR LB + 50μg/ml ALA IH85 BT3 hemG F- su0 hemG metam3 lac1000 trpam strr bfeam tsxam JC7623 hemG BT3 P1-transduction hemG::KmR LB + 0.2 % glucose IH86 CR63 hemG F+ supF λR hemG JC7624 hemG CR63 P1-transduction hemG::KmR LB + 0.2 % glucose IH87 BT13 hemG F- lac1000 hemG trpam metam3 strr bfeam tsxam supD JC7625 hemG BT13 P1-transduction hemG::KmR LB + 0.2 % glucose IH88 LG285 F- supE44 galK2 supF58 metB1 trpR55 lacY1 galT22 HsdR514 hemG JC7626 hemG LE392 P1-transduction hemG::KmR LB + 0.2 % glucose IH89 BT3 hemA F- metam3 tsxam trpam hemA lac1000 strr su0 bfeam LE392ΔhemA BT3 P1-transduction hemA::KmR LB + 50μg/ml ALA IH90 BT13 hemA F- lac1000 supD hemA trpam metam3 strr bfeam tsxam LE393ΔhemA BT13 P1-transduction hemA::KmR LB + 50μg/ml ALA IH91 VS207 F+ hemCD HfrC su0 trpam lacam125 visA(=ΔhemH) VS200 Light-resistant hem CD LB + 0.2 % glucose A point mutant in the hemCD operon. Complemented with Kohara clone of 12G1. IH92 LK783 F- supE44 hemK lacY1 metB1 galT22 galK2 trpR55 HsdR514 supF58 LE392 in vitro & P1(*)) hemK::CmR LB + 0.2 % glucose A disrupted strain of the gene of hemK. (*)in vitro gene manipulation->transformation to the strain JC7623->P1 transduction. IH93 9829 hemE F- hemE tyram trpam JC7624hemE 9829 P1-transduction hemE::CmR LB + 0.2 % glucose IH94 BT52 hemE F- strr hemE lac1000 trpam bfeam tsxam JC7624hemE BT52 P1-transduction hemE::CmR LB + 0.2 % glucose IH95 BT63 ts29ΔssrA F- Other λ miaA metam3 trpam lac1000 strr bfeam ΔssrA supP tsxam W3110ΔssrA BT63 ts29 P1-transduction ΔssrA::KmR ordinary conditions (LB 30degreeC) The ts29 phenotype is complementated by introduction of the wild-type leuX and Kohara clone #652 (miaA+) IH96 BT63 ts39ΔssrA F- Other λ gidA tsxam bfeam strr metam3 trpam lac1000 ΔssrA supP W3110ΔssrA BT63 ts39 P1-transduction ΔssrA::KmR ordinary conditions (LB 30degreeC) The ts39 phenotype is complementated by introduction of the wild-type leuX and Kohara clone #560 (gidA+) IH99 CATL-d1 F- Other λ W3110 NTG & PS(*) dependent mutation LB + 250μg/ml Catalase Catalase-dependent mutant; a mutation in glnA (F80S). (*)penicillin-screening IH100 CATL-d4 F- Other λ W3110 NTG & PS(*) dependent mutation LB + 250μg/ml Catalase Catalase-dependent mutant; a mutation in glnA. (*)penicillin-screening IH101 SAPO-d1 F- Other λ W3110 NTG & PS(*) dependent mutation LB + 100μg/ml Saponin Saponin-dependent mutant; a mutation in hflB. (*)penicillin-screening IH102 SAPO-d3 F- Other λ W3110 NTG & PS(*) dependent mutation LB + 100μg/ml Saponin Saponin-dependent mutant; a mutation in hflB. (*)penicillin-screening IH103 VALP-d1 F- Other λ W3110 NTG & PS(*) dependent mutation LB Valproate-dependent mutant; a mutation in cysS. (*)penicillin-screening IH104 VALP-d2 F- Other λ W3110 NTG & PS(*) dependent mutation LB Valproate-dependent mutant; a mutation in asnS. (*)penicillin-screening IH105 LS653 (pMB1) F- Other ilv- thyA- metB- argH- strr rifR lacZUGA659 leuUGA trpAam9605 hisam29 LS653 transformation colicin production Kyoto University ordinary conditions (LB 37degreeC) The plasmid is carrying colicine E1 and the genes for restriction and modification of Eco RI. IH106 HfrH (colE1Ap) Hfr Other colE1 Tn3(ApR) HfrH transformation ApR Kyoto University ordinary conditions (LB 37degreeC) IH107 R15 F- su0 Δattλ uvrB- gal- W3350 excision of λint- xis- uvr- λ- ordinary conditions (LB 37degreeC) A deletion mutant strain of attλ. IH110 W3110 glnS1 F- glnS1 AB4143 W3110 marker exchange with transducing phage temperature sensitivity ordinary conditions (LB 37degreeC) A ts mutant of glutaminyl-tRNA synthetase. IH111 W3110 glnS1ΔssrA F- glnS1 ΔssrA W3110ΔssrA W3110glnS1 P1-transduction ΔssrA::KmR ordinary conditions (LB 37degreeC) A ts mutant of glutaminyl-tRNA synthetase, and ΔssrA. IH112 CR63 glnS1 F+ supD glnS1 λR CR63 marker exchange with transducing phage temperature sensitivity ordinary conditions (LB 37degreeC) A ts mutant of glutaminyl-tRNA synthetase. IH113 CR63 glnS1 ΔssrA F+ supD glnS1 ΔssrA λR W3110ΔssrA CR63glnS1 P1-transduction ΔssrA::KmR ordinary conditions (LB 37degreeC) A ts mutant of glutaminyl-tRNA synthetase, and ΔssrA. IH114 WCA167 Hfr lacI22 sup0 thi-1 lacZ13 CA167 supC70 spontaneous mutation ordinary conditions (LB 37degreeC) IH115 WCA167ΔssrA Hfr lacZ13 ΔssrA sup0 lacI22 thi-1 W3110ΔssrA WCA167 P1-transduction ΔssrA::KmR ordinary conditions (LB 37degreeC) IH116 WCA167supF Hfr Other φ80psu+3 supF thi-1 lacI22 lacZ13 WCA167 lysogenization of φ80psu+3 φ80 immunity ordinary conditions (LB 37degreeC) IH117 WCA167ΔssrA supF Hfr Other φ80psu+3 lacZ13 ΔssrA supF thi-1 lacI22 WCA167ΔssrA lysogenization of φ80psu+3 φ80 immunity ordinary conditions (LB 37degreeC) IH69 JC7623 hemF F- hemF JC7623 in vitro & transformation hemF::KmR from Dr. Shin-ichiro Narita LB + 0.2 % glucose IH70 LE392 hemN F- hemN JC7623hemN LE392 in vitro & P1(*) hemN::CmR from Dr. Shin-ichiro Narita LB + 0.2 % glucose (*)in vitro manuplation of the gene->transformation to the strain JC7623->P1 transduction IH97 LF375 F- hemF JC7623hemF LE392 in vitro & P1(*) hemF::KmR from Dr. Shin-ichiro Narita LB + 0.2 % glucose (*)in vitro manuplation of the gene->transformation to the strain JC7623->P1 transduction IH98 CK783 Hfr hemK JC7823hemK CA293 in vitro & P1(*) hemK::CmR from Dr. Shin-ichiro Narita LB + 0.2 % glucose (*)in vitro manuplation of the gene->transformation to the strain JC7623->P1 transduction IH118 W3110 ts 01-03 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 219.
asnS(220?) IH119 W3110 ts 01-04 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 514.515.516. IH120 W3110 ts 01-08 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 472.
fba IH121 W3110 ts 01-11 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 238.
lolC,D,E IH122 W3110 ts 01-14 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 322.323.
pheT,S,rplT,rpmI,infC IH123 W3110 ts 01-21 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 571.572.
gmL,spoT IH124 W3110 ts 02-04 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 417.
liq,zipA IH125 W3110 ts 02-10 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 152.153.
adK IH126 W3110 ts 02-45 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 615.616. IH127 W3110 ts 02-52 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 301.302. IH128 W3110 ts 03-17 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 120.
map,rpsB,pvrB,frr IH129 W3110 ts 04-17 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 546.547. IH130 W3110 ts 04-33 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 166,167 IH131 W3110 ts 04-64 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 222.
fabA IH132 W3110 ts 04-66 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 121.
lpxD,hlpA,fabZ IH133 W3110 ts 04-76 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 109.110.
ftsL,I,murE,etc. IH134 W3110 ts 05-16 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 323.324.
thrS IH135 W3110 ts 05-36 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 438.439
rplS,trmD,rpsP,ffh,grpE IH136 W3110 ts 05-84 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 558 IH137 W3110 ts 06-22 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 625. IH138 W3110 ts 06-29 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 333.334. IH139 W3110 ts 06-42 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 101.102. IH140 W3110 ts 06-75 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 560. IH141 W3110 ts 07-87 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 551. IH142 W3110 ts 08-09 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 103.
lspA,lytB,(ileS) IH143 W3110 ts 08-14 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 157.158.
cysS IH144 W3110 ts 09-14 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 102.103.
ileS IH145 W3110 ts 09-28 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 337
aspS IH146 W3110 ts 09-31 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 215.
ftsK,lonA,serS IH147 W3110 ts 14-01 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 658 IH148 W3110 ts 14-06 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 626.
fusA,rpsG,L IH149 W3110 ts 14-15 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 423.424. IH150 W3110 ts 14-21 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 239.240.
asuE,ycfB IH151 W3110 ts 15-31 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 449.450. IH152 W3110 ts 16-07 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 122.
lpxB,dnaE,accA IH153 W3110 ts 17-04 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 534.
murI,B,secE,nusG,rplK,etc. IH154 W3110 ts 17-14 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 158. IH155 W3110 ts 17-22 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 407.408.
fabB IH156 W3110 ts 17-36 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 636.
dnaB IH157 W3110 ts 18-09 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 612.613.
ftsE,Y IH158 W3110 ts 18-21 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 529.
accC (accB?) IH159 W3110 ts 18-25 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 604.605. IH160 W3110 ts 18-31 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 601.
glyS,glyQ IH161 W3110 ts 18-37 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 555.556.
rhoL,rho IH162 W3110 ts 18-51 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 246. IH163 W3110 ts 22-10 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 427.428.
hisS IH164 W3110 ts 22-13 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 573.
kdtA,coaD,rpmG,rpmB,etc. IH165 W3110 ts 22-17 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 157. IH166 W3110 ts 22-18 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 416. IH167 W3110 ts 22-34 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 456.457 IH168 W3110 ts 23-33 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 172.
glnS IH169 W3110 ts 23-50 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 671.672.
dnaC,T IH170 W3110 ts 24-21 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 302. IH172 W3110 ts 25-21 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 617.618. IH173 W3110 ts 25-31 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 255.256.
fabI IH174 W3110 ts 25-37 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 110.111
murD,G,C,ftsW,Q,A IH175 W3110 ts 26-21 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 322. IH176 W3110 ts 26-36 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 520.
infB,nufA,etc? IH177 W3110 ts 27-09 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 312-314 IH178 W3110 ts 27-36 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 403.404. IH179 W3110 ts 28-01 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 502506. IH180 W3110 ts 28-12 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 247.248.
prfA, kdsA IH181 W3110 ts 28-3B F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 340
pqsA(343?) IH182 W3110 ts 29-09 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 634635. IH183 W3110 ts 29-13 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 316.
tyrS IH184 W3110 ts 30-19 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 554. IH185 W3110 ts 30-35 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 176,175 IH186 W3110 ts 30-52 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 146.
nusB,dxs,ispA IH187 W3110 ts 31-39 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 166167. IH188 W3110 ts 32-02 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 506
plsC, parC IH189 W3110 ts 32-19 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 153. IH190 W3110 ts 32-28 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 217.
rpsA,msbA IH191 W3110 ts 32-40 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 505.514.516 IH192 W3110 ts 32-42 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 455.
eno IH193 W3110 ts 32-55 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 520.521.
mrsA,hfl,rrmI IH194 W3110 ts 33-02 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 446.
alaS IH195 W3110 ts 33-09 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 521.
rpmA,rplU,murA IH196 W3110 ts 33-13 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 246.247.
pth,prs,hemM IH197 W3110 ts 33-24 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 235.236.
fabD,fabG,acpP,tmk,holB IH198 W3110 ts 33-35 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 565.
dnaN IH199 W3110 ts 33-37 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 223.224. IH200 W3110 ts 33-40 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 416.417.
gltX IH201 W3110 ts 33-46 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 418 IH202 W3110 ts 33-48 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 341 IH203 W3110 ts 34-05 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 564.565
dnaA,rpmH,rnpA,yidC IH204 W3110 ts 34-17 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 169.
leuS,holA IH205 W3110 ts 35-17 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 658.659.
valS IH206 W3110 ts 35-22 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 326.327.
nadE IH207 W3110 ts 35-27 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 460.461.
lgt IH208 W3110 ts 35-28 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 622.623.
trpS IH209 W3110 ts 35-30 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 175. IH210 W3110 ts 35-35 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 509.
ygjD,dnaG IH211 W3110 ts 35-40 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 119. IH212 W3110 ts 35-41 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC 121.122
lpxB,dnaE,accA IH213 W3110 ts 35-47 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ts phenotype is complemented with infection of Kohara phage clone 122.123.
proS IH214 W3110 ts 25-18 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC IH215 W3110 ts 32-27 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC IH216 W3110 ts 34-21 F- Other λ ts W3110 NTG & PS(*) ts LB 30degreeC ME9620 SP45 F- tyr trp pbpA45(Ts) supD126 ilv W3110 grow at 30°C 29809 ME9621 MC1061 F- araD139 ΔlacX74 strA hsm+ hsr galK galU Δ(ara leu)7697 LB broth The parental strain for ME9622-9626, ME9628 30140 ME9622 SY2 F- MC1061 sulA::lacZ'YA::kan MC1061 gene disruption method (Ohmori et al.,1995, J. Bacteriol. 177: 156-165) LB broth useful for measurement of SOS induction 30126 ME9623 SY52 F- Δ(dinG-ybiB)101::kan MC1061 MC1061 gene disruption method (Ohmori et al.,1995, J. Bacteriol. 177: 156-165) LB broth 30140 ME9624 SY117 F- MC1061 dinI1::kan MC1061 gene disruption method (Ohmori et al.,1995, J. Bacteriol. 177: 156-165) LB broth 30140 ME9625 SY181 F- (λC43 dinD::lacZ)+ MC1061 MC1061 λ phage LB broth 30150 ME9626 SY183 F- ΔdinI2::kan MC1061 MC1061 gene disruption method (Ohmori et al.,1995, J. Bacteriol. 177: 156-165) LB broth 30150 ME9627 SY184 F- zjb564::Tn10 SY2 lexA51(Def) KH07 SY2 P1 transduction LB broth 30150 ME9628 KN2012 F- MC1061 zjb564::Tn10 lexA1(Ind-) AB2494 MC1061 P1 transduction LB broth sensitive to DNA damage 30126 ME9629 HB101 (pgnt41) galK2 lacY1 mtl-1 hsdS20(r-B,m-B) xyl-5 supE44 rpsL20 recA13 ara-14 proA2 ampicilin-r L medium 29814 ME9630 HB101 (pLS353) lacY1 xyl-5 supE44 rpsL20 ara-14 galK2 hsdS20(r-B,m-B) mtl-1 proA2 recA13 ampicilin-r L medium 29847 ME9631 JM109 (pUC118) thi endA1 hsdR17 recAl supE44 gryA96 F'[traD36 proAB+ lacIq lacZΔM15] Δ(lac-proAB) ampicilin-r L medium 29792 ME9632 JM109 (pIOL02) recAl gryA96 F'[traD36 proAB+ lacIq lacZΔM15] Δ(lac-proAB) thi supE44 hsdR17 endA1 ampicilin-r L medium 29792 ME9633 JM109 (pIOL05) endA1 hsdR17 recAl supE44 thi Δ(lac-proAB) F'[traD36 proAB+ lacIq lacZΔM15] gryA96 ampicilin-r L medium 29792 ME9634 HB101 (pBC275HE) ara-14 galK2 hsdS20(r-B,m-B) lacY1 mtl-1 proA2 recA13 rpsL20 supE44 xyl-5 ampicilin-r L medium 30115 ME9635 HB101 (pSOFT2) ara-14 galK2 hsdS20(r-B,m-B) lacY1 mtl-1 proA2 recA13 rpsL20 supE44 xyl-5 ampicilin-r L medium 30115 ME9636 HB101 (pSOFT11) ara-14 galK2 hsdS20(r-B,m-B) lacY1 mtl-1 proA2 recA13 rpsL20 supE44 xyl-5 ampicilin-r L medium 30115 ME9637 JM109 (pgnt54) supE44 endA1 hsdR17 recAl thi Δ(lac-proAB) F'[traD36 proAB+ lacIq lacZΔM15] gryA96 ampicilin-r L medium 30133 ME9638 JM109 (pGNT61) endA1 gryA96 F'[traD36 proAB+ lacIq lacZΔM15] Δ(lac-proAB) thi supE44 recAl hsdR17 tetracycline-r L medium 30125 ME9639 JM109 (pGNT61 pCCPA19) F'[traD36 proAB+ lacIq lacZΔM15] Δ(lac-proAB) thi supE44 recAl hsdR17 endA1 gryA96 tetracycline-r, ampicillin-r L medium 30125 ME9640 JM109 (pAG58) gryA96 Δ(lac-proAB) thi supE44 recAl hsdR17 endA1 F'[traD36 proAB+ lacIq lacZΔM15] ampicillin-r L medium 30160 ME9641 C600 (ptrpBGI) thi-1 supE44 leuB6 lacY1 hsdR tonA21 ampicillin-r L medium 30160 ME9642 XL1-BLUE(pFBP119) lac/F'[proAB+, lac Iq, lacZΔM15:: Tn10(tetr)] hsdR17 gryA96 thi supE44 relA1 recAl endA1 ampicillin-r L medium 30151, 33597 ME9643 DH5α(pDG148) F- supE44 F- gyrA96 phoA hsdR17(rk- mk+) thi-1 relA1 recAl endA1 Δ(lacZYA-argF)U169 λ- φ80d lacZΔM15 deoR ampicillin-r L medium 30179, 84959 ME9644 JM109(pMUTIN1) hsdR17 recAl supE44 gryA96 F'[traD36 proAB+ lacIq lacZΔM15] thi Δ(lac-proAB) endA1 ampicillin-r L medium 30180 ME9645 JM109(pMUTIN2) endA1 hsdR17 recAl supE44 thi Δ(lac-proAB) gryA96 F'[traD36 proAB+ lacIq lacZΔM16] ampicillin-r L medium 30161 ME9646 JM109(pCRE-test) endA1 hsdR17 recAl supE44 thi Δ(lac-proAB) gryA96 F'[traD36 proAB+ lacIq lacZΔM17] ampicillin-r L medium 30160 ME9647 JM109 (pCRE-test2) Δ(lac-proAB) thi supE44 recAl hsdR17 endA1 gryA96 F'[traD36 proAB+ lacIq lacZΔM18] ampicillin-r L medium 33537 ME9648 JM109(pTC177) supE44 F'[traD36 proAB+ lacIq lacZΔM15] [plasmid; Tc Ori-177] gryA96 Δ(lac-proAB) endA1 hsdR17 thi recAl tetracycline-r L medium 30136 ME9649 JM109(pGNT177) F'[traD36 proAB+ lacIq lacZΔM15] [plasmid; cat(Pgnt), Tc ori-177] endA1 hsdR17 recAl supE44 Δ(lac-proAB) gryA96 thi tetracycline-r L medium 30136 ME9650 JM109(pGNT81,pGNT177) endA1 thi Δ(lac-proAB) gryA96 F'[traD36 proAB+ lacIq lacZΔM15] [plasmid1; gntR(Pgnt) Ap (pUC19), plasmid2; cat(Pgnt) Tc ori-177 (pTC177)] recAl supE44 hsdR17 tetracycline-r, ampicillin-r L medium 30136 ME9651 JM109(pGNT81(43L),pGNT177) hsdR17 F'[traD36 proAB+ lacIq lacZΔM15] [plasmid1; gntR43L(Pgnt) Ap (pUC19), plasmid2; cat(Pgnt)Tc ori-177 (pTC177)] gryA96 Δ(lac-proAB) thi supE44 recAl endA1 tetracycline-r, ampicillin-r L medium 30136 ME9652 JM109(pGNT81(66T),pGNT177) F'[traD36 proAB+ lacIq lacZΔM15] [plasmid1; gntR66T(Pgnt) Ap (pUC19), plasmid2; cat(Pgnt)Tc ori-177 (pTC177)] endA1 hsdR17 recAl supE44 thi Δ(lac-proAB) gryA96 tetracycline-r, ampicillin-r L medium 30136 ME9654 JM109(pGNT81(75Q),pGNT177) gryA96 endA1 hsdR17 recAl supE44 thi Δ(lac-proAB) F'[traD36 proAB+ lacIq lacZΔM15] [plasmid1; gntR75Q(Pgnt) Ap (pUC19), plasmid2; cat(Pgnt)Tc ori-177 (pTC177)] tetracycline-r, ampicillin-r L medium 30136 ME9655 BL21[DE3] F- λDE3 DE3 (T7 RNA polymerase/PlacUV5) gal hsdS(rB- mB-) F- L medium 30185 ME9656 JM109 (pIOLR3) gryA96 endA1 hsdR17 recAl supE44 thi Δ(lac-proAB) F'[traD36 proAB+ lacIq lacZΔM15] [plasmid; iolR (Plac) Ap, pUC19) ampicillin-r L medium 30185 ME9657 ME6279 (pOU71) F- str asnA asnB thi thy F- recA[plasmid; amp low copy-number] ampicillin-r L medium 33533 ME9658 ME6279 (pASNB) F- recA[plasmid; asnB, pOU71] asnA asnB str thi thy F- ampicillin-r L medium 33533 ME9659 ME6279 (pASNH) F- asnB asnA recA[plasmid; asnH, pOU71] F- thy thi str ampicillin-r L medium 33533 ME9660 ME6279 (pASNO) F- asnB asnA recA[plasmid; asnO, pOU71] F- thy thi str ampicillin-r L medium 33533 ME9661 JM109 (pIOLB) recAl supE44 hsdR17 endA1 F'[traD36 proAB+ lacIq lacZΔM15][plasmid; Plac-iolB, pUC18] gryA96 Δ(lac-proAB) thi ampicillin-r L medium 30180 ME9662 JM109 (pIOLC) recAl endA1 F'[traD36 proAB+ lacIq lacZΔM15][plasmid; Plac-iolC, pUC18] gryA96 Δ(lac-proAB) thi supE44 hsdR17 ampicillin-r L medium 30180 ME9663 JM109 (pIOLD) gryA96 endA1 hsdR17 recAl supE44 thi Δ(lac-proAB) F'[traD36 proAB+ lacIq lacZΔM15][plasmid; Plac-iolD, pUC18] ampicillin-r L medium 30180 ME9664 JM109 (pIOLE) thi supE44 recAl hsdR17 endA1 F'[traD36 proAB+ lacIq lacZΔM15][plasmid; Plac-iolE, pUC18] gryA96 Δ(lac-proAB) ampicillin-r L medium 30185 ME9665 JM109 (pLMRA) recAl endA1 hsdR17 gryA96 F'[traD36 proAB+ lacIq lacZΔM15] Δ(lac-proAB) thi supE44 ampicillin-r L medium 30181 ME9666 BL21(DE3) (pET-QdoR) F- λDE3 hsdS(rB- mB-) DE3 (T7 RNA polymerase/PlacUV5) F- gal ampicillin-r L medium 30187 ME9696 ON112 F- thi endA rna valr metE proA supE 34756 ME9697 ON121 F- proA+ rnhA59 ON112 34756 ME9698 ON152 F- rnhA91 proA+ ON112 34756 ME9699 ON2104 F- polA4113 metE+ ON152 34756 ME9700 KN72 F- polA4113 metE+ ON112 34760 ME9701 DPB271 F- recD::mini-tet 34758 ME9702 RSD497 F- ΔdatA::kan DPB271 Km 34758 ME9703 W3110 F- rph-1 IN(rrnD-rrnE)1, 34758 ME9704 RSD448 F- ΔdatA::kan W3110 Km 34758 ME9705 RSD561 F- (= datAΔbox2) datA1 W3110 34758 ME9706 B/rF26 F- his thyA 34758 ME9707 RSD338 F- ΔdatA::kan B/rF26 Km 34758 ME9708 RSD411 F- ΔdatA::kan zae502::Tn10datA:cat RSD421 Cm, Km 34758 ME9709 RSD412 F- ΔdatA::kan zbc3105::Tn10datA:cat RSD422 Cm, Km 34758 ME9710 RSD413 F- zce726::Tn10datA:cat ΔdatA::kan RSD423 Cm, Km 34758 ME9711 RSD414 F- ΔdatA::kan zde234::Tn10datA:cat RSD424 Cm, Km 34758 ME9712 RSD415 F- ΔdatA::kan zee3129::Tn10datA:cat RSD425 Cm, Km 34758 ME9713 RSD416 F- ΔdatA::kan zff208::Tn10datA:cat RSD426 Cm, Km 34758 ME9714 RSD417 F- ΔdatA::kan zgd210::Tn10datA:cat RSD427 Cm, Km 34758 ME9715 RSD418 F- zhe3085::Tn10datA:cat ΔdatA::kan RSD428 Cm, Km 34758 ME9716 RSD419 F- ΔdatA::kan zie296::Tn10datA:cat RSD429 Cm, Km 34758 ME9717 RSD420 F- ΔdatA::kan zje2241::Tn10datA:cat RSD430 Cm, Km 34758 ME9718 RSD421 F- ΔdatA::kan zae502::Tn10 CAG18436 Km 34758 ME9719 RSD422 F- zbc3105::Tn10 ΔdatA::kan CAG12021 Km 34758 ME9720 RSD423 F- ΔdatA::kan zce726::Tn10 CAG12078 Km 34758 ME9721 RSD424 F- ΔdatA::kan zde234::Tn10 CAG18459 Km 34758 ME9722 RSD425 F- zee3129::Tn10 ΔdatA::kan CAG12099 Km 34758 ME9723 RSD427 F- ΔdatA::kan zgd210::Tn10 CAG12168 Km 34758 ME9724 RSD428 F- ΔdatA::kan zhe3085::Tn10 CAG18452 Km 34758 ME9725 RSD429 F- ΔdatA::kan zie296::Tn10 CAG18501 Km 34758 ME9726 RSD430 F- zje2241::Tn10 ΔdatA::kan CAG18427 Km 34758 ME9727 RSD565 F- datAΔbox1 W3110 34762 ME9728 RSD567 F- datAΔbox5 W3110 34762 ME9729 RSD590 F- 5 4, datAΔbox1, W3110 34762 ME9730 RSD564 F- datAΔbox1-5 W3110 34762 ME9731 MC1061 F- mcrB1 araD139 Δ(araA-leu)7697 Δ(codB-lacI)3 galK16 galE15 LAM- e14- mcrA0 relA1 rpsL150 spoT1 hsdR2 34761 ME9732 dnaAΔ[87-104] F- dnaAΔ[87-104] MC1061 34761 ME9733 dnaAΔ[79-108] F- dnaAΔ[79-108] MC1061 34761 ME9734 dnaAΔ[81-110] F- dnaAΔ[81-110] MC1061 34761 ME9735 dnaAΔ[82-111] F- dnaAΔ[82-111] MC1061 34761 ME9736 dnaAΔ[83-112] F- dnaAΔ[83-112] MC1061 34761 ME9737 dnaAΔ[85-114] F- dnaAΔ[85-114] MC1061 34761 ME9738 dnaAΔ[88-117] F- dnaAΔ[88-117] MC1061 34761 ME9739 dnaAΔ[91-120] F- dnaAΔ[91-120] MC1061 34761 ME9740 dnaAΔ[94-123 F- dnaAΔ[94-123] MC1061 34761 ME9741 dnaAΔ[97-126] F- dnaAΔ[97-126] MC1061 34761 ME9742 dnaAΔ[98-132] F- dnaAΔ[98-132] MC1061 34761 ME9743 dnaAΔ[99-134] F- dnaAΔ[99-134] MC1061 34761 ME9744 dnaAΔ[100-135] F- dnaAΔ[100-135] MC1061 34761 ME9745 dnaAΔ[106-135] F- dnaAΔ[106-135] MC1061 34761 ME9746 datAcore F- datAcore W3110 34757 ME9747 datAdelA F- datAdelA W3110 34757 ME9748 datAcore+delA F- datAcore+delA W3110 34757 ME9749 datAdelB F- datAdelB W3110 34757 ME9750 datAdelC F- datAdelC W3110 34757 ME9751 datAdelBC F- datAdelBC W3110 34757 ME9752 datAdelD F- datAdelD W3110 34757 ME9753 datAinv2 F- datAinv2 W3110 34757 ME9754 datAinv3 F- datAinv3 W3110 34757 ME9755 datAinsA F- datAinsA W3110 34757 ME9756 datAinsB F- datAinsB W3110 34757 ME9757 datAinsA+ F- datAinsA+ W3110 34757 ME9758 datAinsB+ F- datAinsB+ W3110 34757 ME9759 ON379 F- hip-6his-FRT-kan-FRT MG1655 Km 34757 ME9760 ON373 F- hip-6his MG1655 34757 ME9761 ON374 F- datAcore hip-6his MG1655 34757 ME9762 dnaA-eyfp tetR-mCherry F- ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-tetR-mCherry) SN101 34757 ME9763 dnaA-eyfp tetR-mCherry / pMW119 tetO array F- ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-tetR-mCherry) SN101 Gen 34757 ME9764 dnaA-eyfp tetR-mCherry / pMW119EX tetO array F- ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-tetR-mCherry) SN101 Gen 34757 ME9765 dnaA-eyfp tetR-mCherry / pMW119EX2 tetO array F- ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-tetR-mCherry) SN101 Gen 34757 ME9766 dnaA-eyfp tetR-mCherry / pMW119EXcore tetO array F- Δ(353820-353822)::(araC pBAD-tetR-mCherry) ΔdnaA::dnaA-eyfp SN101 Gen 34757 ME9767 SN109 F- Δ(353820-353822)::(araC pBAD-lacI-ecfp) ori1:lacO array MG1655 Km 34757 ME9768 SN110 F- datAΔbox1-5 array ori1:lacO Δ(353820-353822)::(araC pBAD-lacI-ecfp) SN109 Km 34757 ME9769 SN101 F- ΔdnaA::dnaA-eyfp MG1655 34759 ME9770 SN111 F- array ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-lacI-ecfp) ori1:lacO SN101 Km 34759 ME9771 SN114 F- dat1:lacO ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-lacI-ecfp) array SN101 Km 34759 ME9772 SN117 F- ter2:lacO ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-lacI-ecfp) array SN101 Km 34759 ME9773 dnaA-eyfp lacI-edfp tetR-mCherry ori1:tetO array dat1:lacO array F- dat1:lacO ΔdnaA::dnaA-eyfp ori1:tetO array ΔyajR::(araC pBAD lacI-ecfp tetR-mCherry FRT-cat-FRT) SN101 Gen, Km, Cm 34759 ME9774 dnaA-eyfp tetR-mCherry / pNZK1 F- Δ(353820-353822)::(araC pBAD-tetR-mCherry) ΔdnaA::dnaA-eyfp SN101 Gen 34759 ME9775 dnaA-eyfp tetR-mCherry / pNZK1-datA F- ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-tetR-mCherry) SN101 Gen 34759 ME9776 dnaA-eyfp tetR-mCherry / pNZK1-datAΔbox2 F- ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-tetR-mCherry) SN101 Gen 34759 ME9777 dnaA-eyfp tetR-mCherry / pNZK1-oriCΔ13-mer F- Δ(353820-353822)::(araC pBAD-tetR-mCherry) ΔdnaA::dnaA-eyfp SN101 Gen 34759 ME9778 SN122 F- ori1::lacO datAΔbox1-5 ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-lacI-ecfp) array SN111 Km 34759 ME9779 dnaA-eyfp lacIecfp dat1:lacO array datAΔbox1-5 F- dat1::lacO datAΔbox1-5 ΔdnaA::dnaA-eyfp Δ(353820-353822)::(araC pBAD-lacI-ecfp) array SN114 Km 34759 ME9780 AI-0714 F- Escherichia coli K12 W3110 type-A This strain was used throughout the Genomic SELEX screening of regulation targets for all sigma subunits and all transcription factors (TFs).
TEC 36734, 50617, 50619, 50621, 36733 ME9781 UE60 F- ∆ara714 ∆(λattL-lom)::(bla araC PBAD-pgsA) ∆pgsA::FRT-kan-FRT MG1655 Amp, Kan 12 mM arabinose, 20 µg/ml Amp, 20 µg/ml Kan UE60 requires arabinose because pgsA is essential gene. ME9782 UE127 F- ∆(λattL-lom)::(bla araC PBAD-pssA) ∆pssA::FRT-kan-FRT ∆ara714 EDCM367 = MG1655 ∆(Plac-lacZY) Amp, Kan 12 mM arabinose, 20 µg/ml Amp, 20 µg/ml Kan "UE127 requires arabinose because ppsA is essential gene. EDCM367 is described in PMID: 12218041" ME9783 SN1071 F- tsx-33 rpsL31 thr-1 leuB6 thi-1 lacY1 galK2 ara-4 xyl-5 mtl-1 proA2 his-60 ΔhsdR::frt sbcA23 recC22 argE3 recB21 supE44 JC8679 (AQ3625) Please refer to the paper (DOI: 10.1128/JB.00660-18.) about the method to construct this strain. L 78983, 54451 ME9784 GN10 /pMS5(pssA+ derivative of pMAN035) ΔpssA10::cam W3110 "The procedure for the construction of the strain is described in the paper entitled \"A regulatory mechanism for the balanced syntheis of membrane phospholipid species in Escherichia coli \" (Biosci. Biotech. Biochim. (1996) 60, 111-116). Curering at 43◦C for 3 hr of covering plasmid pMS5 (pssA+) in LB medium containing 50mM MgCl2, confirm Ap-sensitivity for the curing." cam, Aps LB medium +50 mM MgCl2 ME9785 GN10Y ΔpssA10::cam /pMS5(pssA+ derivative of pMAN035) lacY::Tn5 W3110 "The procedure for the construction of the strain is described in the paper entitled \"A regulatory mechanism for the balanced syntheis of membrane phospholipid species in Escherichia coli \" (Biosci. Biotech. Biochim. (1996) 60, 111-116).Curering at 43◦C for 3 hr of covering plasmid pMS5 (pssA+) in LB medium containing 50mM MgCl2, confirm Ap-sensitivity for the curing and of lacY::Tn5. " cam, Kan, Aps LB +50 mM MgCl2 ME9786 S301 ksgB1lpp-2 (formerly lpo) W3110 "The procedure for the construction of the strain is described in the paper entitled \"Viability of an Escherichia coli pgsA null mutant lacking detectable phosphatidylglycerol and cardiolipin\" (J. Bacteriol. (2000) 182, 371-376)." LB medium 48085 ME9787 S303 uvrC::ΔTn10 ksgB1lpp-2 pgsA3 W3110 "The procedure for the construction of the strain is described in the paper entitled \"Viability of an Escherichia coli pgsA null mutant lacking detectable phosphatidylglycerol and cardiolipin\" (J. Bacteriol. (2000) 182, 371-376)." LB medium 48085 ME9788 S330 lpp-2 rcsA330::IS5 ksgB1 pgsA30::kan W3110 "The procedure for the construction of the strain is described in the paper entitled \"Viability of an Escherichia coli pgsA null mutant lacking detectable phosphatidylglycerol and cardiolipin\" (J. Bacteriol. (2000) 182, 371-376). P1transduction of pgsA::kan from MDL12." kan LB medium 30°C 48085 ME9789 YS330 pgsA30::kan ksgB1 pp-2 rcsA+ W3110 "The procedure for the construction of the strain is described in the paper entitled \"RcsA-dependent and -independent growth defects caused by the activated Rcs phosphorelay system in the Escherichia coli pgsA null mutant. J.Gen. Appl. Microbiol. (2006) 52, 91-98. P1transduction of pgsA::kan from MDL12 (at 30◦C)." kan LB medium 30°C 3287 ME9790 OS2101 rpsL118 pssA1pyrD34 glpKp glpK glpR2 glpD3 phoA8 galK35 thyA33 W3110 "The procedure for the construction of the strain is described in the paper entitled \"Membrane phospholipid synthesis and phenotypic correlation of an Escherichia coli pss mutant\" (J. Bacteriol. (1977) 132, 434-443. " NBY medium temperature-sensitive 48082 ME9791 R4 of TS-revertant OS2101 W3110 "The procedure for the construction of the strain is described in the paper entitled \"Membrane phospholipid synthesis and phenotypic correlation of an Escherichia coli pss mutant\" (J. Bacteriol. (1977) 132, 434-443. " NBY medium temperature-sensitive 48082 ME9792 R6 TS-revertant of OS2101 W3110 "The procedure for the construction of the strain is described in the paper entitled \"Membrane phospholipid synthesis and phenotypic correlation of an Escherichia coli pss mutant\" (J. Bacteriol. (1977) 132, 434-443. " NBY medium 48082 ME9793 S107 pssA1 phe::ΔTn10 W3110 "The procedure for the construction of the strain is described in the paper entitled \"Membrane phospholipid synthesis and phenotypic correlation of an Escherichia coli pss mutant\" (J. Bacteriol. (1977) 132, 434-443. " NBY medium 48082 ME9794 SD12-CLR galK35 pyrD34 Δcls::kan rpsL118 glpKp glpK glpR2 glpD3 phoA8 his-68 W3110 "The procedure for the construction of the strain is described in the paper entitled \"Disruption of the Escherichia coli cls gene responsible for cardiolipin synthesis\" (J. Bacteriol. (1988) 170, 775-780." LB medium 48083 ME9795 SD12-CLI cls::kan rpsL118 glpKp glpK glpR2 glpD3 phoA8 galK35 his-68 pyrD34 W3110 "The procedure for the construction of the strain is described in the paper entitled \"Disruption of the Escherichia coli cls gene responsible for cardiolipin synthesis\" (J. Bacteriol. (1988) 170, 775-780." LB medium 48083 ME9796 DS1317 mreB-mCherrySW ΔyhdE::cat BW25113 The procedure for the construction of the strain is described in the paper by Kawazura et al., 2017 MolMicro. Cm L 48547 ME9797 DS1322 ΔyhdE mreB-mCherrySW DS1317 The procedure for the construction of the strain is described in the paper by Kawazura et al., 2017 MolMicro. Cm sensitive L 48547 ME9798 RU305 mreB-mCherrySW ΔcsrD::cat ΔyhdE DS1322 The procedure for the construction of the strain is described in the paper by Kawazura et al., 2017 MolMicro. Cm L 48547 ME9799 RU306 ΔcsrD::kan mreB-mCherrySW ΔyhdE DS1322 The procedure for the construction of the strain is described in the paper by Kawazura et al., 2017 MolMicro. Kan L 48547 ME9800 RU331 ΔmreB::cat BW25113 The procedure for the construction of the strain is described in the paper by Kawazura et al., 2017 MolMicro. Cm M9 glucose 48547 ME9801 RU336 ΔmreB BW25113 The procedure for the construction of the strain is described in the paper by Kawazura et al., 2017 MolMicro. Cm sensitive M9 glucose 48547, 56346 ME9802 RU597 mreB-mCherrySW ΔcsrD::cat BW25113 The procedure for the construction of the strain is described in the paper by Kawazura et al., 2017 MolMicro. Cm L 48547 ME9803 RU600 mreB-D83Y-mCherrySW ΔcsrD::cat BW25113 The procedure for the construction of the strain is described in the paper by Kawazura et al., 2017 MolMicro. Cm L 48547 ME9804 RU856 ΔcsrD::kan mreB-mCherrySW MG1655 The procedure for the construction of the strain is described in the paper by Kawazura et al., 2017 MolMicro. Kan L 48547 ME9805 RU857 rcsF::miniTn10cam Δara714 ΔclsA, lpp2 ΔymdB ΔcsrD::kan mreB-mCherrySW ΔpgsA ΔclsC ΔclsB, MG1655 The procedure for the construction of the strain is described in the paper by Kawazura et al., 2017 MolMicro. Kan Kan, Cm L 48547 ME9806 SN1187 F- ΔendA ΔrecA ΔhsdR MG1655 S. Nozaki A strain for iVEC (iVEC3). Patent pending. 54451, 74355, 59194, 59196 ME9807 DS1083 rodZ-3xFLAG BW25113 The procedure for the construction of the strain is described in the paper by Ikebe et al., 2018 Gens to Cells Cm 50384 ME9808 RU383 sfGFP-rodZ BW25113 The procedure for the construction of the strain is described in the paper by Ikebe et al., 2018 Gens to Cells Cm sensitive 50384 ME9809 DS192 ΔmreB MC4100 The procedure for the construction of the strain is described in the paper by Ikebe et al., 2018 Gens to Cells Cm sensitive 50384 ME9810 RU764 sfGFP-rodZΔ134-155 BW25113 The procedure for the construction of the strain is described in the paper by Ikebe et al., 2018 Gens to Cells Cm sensitive 50384 ME9811 RU802 sfGFP-rodZΔ134-180 BW25113 The procedure for the construction of the strain is described in the paper by Ikebe et al., 2018 Gens to Cells Cm sensitive 50384 ME9812 DS290 ∆rodZ::kan JW2500 (∆rodZ::kan) BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 Kan L 52865 ME9813 DS339 ∆rodZ::Kan ispA-A164T BW25113 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 Kan L 52866 ME9814 DS343 ∆rodZ DS290 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 Kan sensitive L 52866 ME9815 DS366 rrfG-1 zff-208::Tn10 rodZWT ME8835 DS338 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 Tet L 52866 ME9816 DS368 rrfG rodZWT zff-208::Tn10 ispAA164T ME8835 DS339 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 kan L 52866 ME9817 DS410 rrfGWT ∆yfiR::kan ispAWT JW2584 DS366 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 kan L 52866 ME9818 DS413 ∆yfiR::kan ispAA164T rrfGWT JW2584 DS368 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 kan sensitive L 52866 ME9819 DS417 ispAWT rrfGWT ∆yfiR DS410 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 kan sensitive L 52866 ME9820 DS419 rrfGWT ispAA164T ∆yfiR DS413 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 kan sensitive L 52866 ME9821 DS426 ∆yfiR ispAWT rrfGWT ∆rodZ::Kan DS417 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 L 52866 ME9822 DS428 ∆yfiR rrfGWT ∆rodZ::Kan ispAA164T DS419 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 kan L 52866 ME9823 DS452 ∆yhdE::cat BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9824 DS453 mreBL17Q ∆yhdE::cat BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9825 DS454 ∆yhdE::cat ∆rodZ::Kan DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9826 DS455 ∆rodZ::Kan mreBL17Q ∆yhdE::cat DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9827 DS552 ∆yhdE::cat mreB-D83Y BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9828 DS554 upstream zipA mutation BW25113 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 L 52867 ME9829 DS559 ∆yhdE::cat mreB-A174T BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9830 DS560 mreB-A174T ∆yhdE::cat ∆rodZ::Kan DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 kan cm L 52865 ME9831 DS561 ∆rodZ::Kan ∆yhdE::cat mreB-G329C DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 kan cm L 52865 ME9832 DS592 ∆mreB::Tn10 (Kan) BW25113 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 kan M9 glucose 52867 ME9833 DS594 ∆rodZ ∆mreB::Tn10 (Kan) DS343 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 kan M9 glucose 52867 ME9834 DS600 ∆pbp2::Tn10 (Kan) BW25113 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 kan M9 glucose 52867 ME9835 DS602 ∆pbp2::Tn10 ∆rodZ (Kan) DS343 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 kan M9 glucose 52867 ME9836 DS612 ∆yhdE::cat mreB-A125V BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9837 DS629 ∆yhdE::cat mreB-D83Y ∆rodZ::Kan DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 kan cm L 52865 ME9838 DS630 ∆rodZ::Kan mreB-A125V∆yhdE::cat DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 kan cm L 52865 ME9839 DS631 ∆rodZ::Kan ∆yfeR::cat zipA upstream DS290 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 kan cm L 52867 ME9840 DS645 ∆yfeR::cat BW25113 BW25113 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 cm L 52867 ME9841 DS673 BW25113 ∆rlpA::cat BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9842 DS674 ∆rodZ::Kan ∆rlpA::cat DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 kan cm L 52865 ME9843 DS679 ∆rodZ::Kan ∆yfeR::cat DS290 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 kan cm L 52867 ME9844 DS684 ∆rlpA::cat pbp2-Q51L BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9845 DS685 pbp2-T52N ∆rlpA::cat BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9846 DS686 ∆rlpA::cat rodA-A234T BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9847 DS687 ∆rlpA::cat rodA-T249P BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9848 DS688 ∆rodZ::Kan ∆rlpA::cat pbp2-Q51L DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 kan cm L 52865 ME9849 DS689 ∆rlpA::cat pbp2-T52N ∆rodZ::Kan DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 kan cm L 52865 ME9850 DS690 ∆rodZ::Kan rodA-A234T ∆rlpA::cat DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 kan cm L 52865 ME9851 DS691 rodA-T249P ∆rlpA::cat ∆rodZ::Kan DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 kan cm L 52865 ME9852 DS708 ∆yfeR::cat WM1074 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 cm L 52867 ME9853 DS709 zipAp ∆yfeR::cat WM1074 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 L 52867 ME9854 DS710 ∆yfeR::cat WM1125 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 cm L 52867 ME9855 DS711 zipAp ∆yfeR::cat WM1125 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 cm L 52865 ME9856 DS797 ∆rlpA BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm sensitive L 52865 ME9857 DS798 pbp2-Q51L ∆rlpA BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm sensitive L 52865 ME9858 DS799 rodA-A234T ∆rlpA BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm sensitive L 52865 ME9859 DS951 zipAP ∆mreB::Tn10 (Kan) ∆yfeR::cat DS592 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 kan cm M9 glucose 52867 ME9860 DS952 zipAP ∆yfeR::cat (Kan) ∆pbp2::Tn10 DS600 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 kan cm M9 glucose 52867 ME9861 DS953 ∆rodZ zipAP ∆yfeR::cat (Kan) ∆mreB::Tn10 DS594 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 kan cm M9 glucose 52867 ME9862 DS954 ∆yfeR::cat ∆pbp2::Tn10 ∆rodZ zipAP (Kan) DS602 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2013 kan cm M9 glucose 52867 ME9863 DS1155 mreB-D83E ∆yhdE::cat BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9864 DS1156 ∆rodZ::Kan ∆yhdE::cat mreB-D83E DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9865 DS1157 ∆yhdE::cat mreB-R124S BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9866 DS1158 ∆yhdE::cat mreB-R124S ∆rodZ::Kan DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9867 DS1159 mreB-R124C ∆yhdE::cat BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9868 DS1160 ∆rodZ::Kan mreB-R124C ∆yhdE::cat DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9869 DS1165 mreB-R127H ∆yhdE::cat BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9870 DS1166 ∆rodZ::KanmreB-R127H ∆yhdE::cat DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9871 DS1171 mreB-E137A ∆yhdE::cat BW25113 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9872 DS1172 ∆yhdE::cat mreB-E137A ∆rodZ::Kan DS290 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm L 52865 ME9873 DS1173 ∆yfiR::kan ispAWT rrfGWTyajO::cat DS410 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 kan cm tet L 52866 ME9874 DS1174 yajO::cat ispAA164T ∆yfiR::kan rrfGWT DS413 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 kan cm tet L 52866 ME9875 DS1186 ∆yajO::cat W3110 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 cm L 52866 ME9876 DS1187 ∆yajO::cat ispA-A164T W3110 The procedure for the construction of the strain is described in the paper by Shiomi and Niki, 2011 cm L 52866 ME9877 DS1324 ∆rlpA pbp2-T52N DS685 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm sensitive L 52865 ME9878 DS1325 rodA-T249P ∆rlpA DS687 The procedure for the construction of the strain is described in the paper by Shiomi et al., 2013 cm sensitive L 52865 ME9884 SN202 F- ΔpanZ::frt MG1655 Please refer to the paper (DOI: 10.1002/mbo3.34.) about the method to construct this strain. L 35431 ME9885 SN205 F- ΔpanD::frt MG1655 Please refer to the paper (DOI: 10.1002/mbo3.34.) about the method to construct this strain. L 35431 ME9886 SN208 F- panD-flag:frt MG1655 Please refer to the paper (DOI: 10.1002/mbo3.34.) about the method to construct this strain. L 35431 ME9887 SN216 F- ΔpanZ::frt panD-flag:frt MG1655 Please refer to the paper (DOI: 10.1002/mbo3.34.) about the method to construct this strain. L 35431 ME9888 SN219 F- ΔpanD::panDBS:frt MG1655 Please refer to the paper (DOI: 10.1002/mbo3.34.) about the method to construct this strain. L 35431 ME9889 SN223 F- ΔpanD::panDBS:frt ΔpanZ::frt MG1655 Please refer to the paper (DOI: 10.1002/mbo3.34.) about the method to construct this strain. L 35431 ME9890 SN220 F- ΔpanD::panDBS-flag:frt MG1655 Please refer to the paper (DOI: 10.1002/mbo3.34.) about the method to construct this strain. L 35431 ME9891 SN224 F- ΔpanZ::frt ΔpanD::panDBS-flag:frt MG1655 Please refer to the paper (DOI: 10.1002/mbo3.34.) about the method to construct this strain. L 35431 ME9892 SN225 F- ΔpanF::kan MG1655 Please refer to the paper (DOI: 10.1002/mbo3.34.) about the method to construct this strain. L 35431 ME9893 SN227 F- hsdS(rB-mB-), gal, ompT, dcm, ΔpanZ::frt (LambdaDE3), BL21(DE3) Please refer to the paper (DOI: 10.1002/mbo3.34.) about the method to construct this strain. L 35431 ME9894 SN1054 F- ΔhsdR::frt MG1655 Please refer to the paper (DOI: 10.1128/JB.00660-18.) about the method to construct this strain. L 54451 ME9895 SN1194 F- ΔhsdR::frt ΔrecA(Δ2,820,759-2,821,785) SN1054 Please refer to the paper (DOI: 10.1128/JB.00660-18.) about the method to construct this strain. L 54451 ME9896 SN1097 F- ΔhsdR::frt ΔrecET::kan SN1054 Please refer to the paper (DOI: 10.1128/JB.00660-18.) about the method to construct this strain. kan L 54451 ME9897 SN1077 F- ΔxthA::kan ΔhsdR::frt SN1054 Please refer to the paper (DOI: 10.1128/JB.00660-18.) about the method to construct this strain. kan L 54451 ME9898 SN1203 F- ΔxthA::kan ΔrecA(Δ2,820,759-2,821,785) ΔhsdR::frt SN1077 Please refer to the paper (DOI: 10.1128/JB.00660-18.) about the method to construct this strain. kan L 54451 ME9899 SN1201 F- ΔhsdR::frt ΔrecET::frt ΔxthA::kan SN1097 Please refer to the paper (DOI: 10.1128/JB.00660-18.) about the method to construct this strain. kan L 54451 ME9900 SN1085 F- ΔhsdR::frt polAΔC::kan SN1054 Please refer to the paper (DOI: 10.1128/JB.00660-18.) about the method to construct this strain. kan L 54451 ME9901 SN1146 F- ΔhsdR(Δ4,581,454-4,584,720) NG1655 Please refer to the paper (DOI: 10.1128/JB.00660-18.) about the method to construct this strain. L 54451 ME9902 SN1171 F- ΔhsdR ΔendA(Δ3,088,303-3,089,037) SN1146 Please refer to the paper (DOI: 10.1128/JB.00660-18.) about the method to construct this strain. L 54451 ME9882 SN1248 F- ΔhsdR ΔlacZ::(T7 gene1:frt) ΔendA MG1655 S. Nozaki Leaky expression of T7 RNA polymerase was observed in L medium without IPTG. Addition of glucose into the medium significantly reduced the leaky expression. Transcription from the T7 promoter can be induced in L medium without glucose or L medium with IPTG. iVEC express (lac), an iVEC strain for protein expression. ME9883 SN1411 F- ΔaraBAD::(T7 gene1) ΔhsdR ΔendA MG1655 S. Nozaki Transcription from the T7 promoter can be induced in L medium with L-arabinose. iVEC express (ara), an iVEC strain for protein expression (arabinose inducible). ME9936 SY2 ∆phr::Cm ∆uvrA::Km ∆recA::Tet JM107 Cm, Km, Tet LB medium is usually available for the cultivation of the strain. However, a minimal synthetic medium, for example, M9 medium, is suitable for selecting F’. "This strain is hypersensitive to UV irradiation because of a defect in repairing pyrimidine dimers.
Data Sheet" 69176 ME9937 SN2099 F- λ+(cI857 Δ(SRRz-cos)::cat) ΔhsdR ΔendA Cm 30°C 73494 ME9938 SN2100 F- ΔhsdR ΔendA λ+(cI857 Δ(SRRz-cos)::kan) Km 30°C 73494 ME9903 W3110ΔompR F- (W3110 typeA←ΔompR) ΔompR W3110, W3110 type A LB medium 59088 ME9904 W3110ΔphoB F- W3110, ΔphoB (W3110 typeA←ΔphoB) W3110 type A LB medium 59088 ME9905 W3110ΔphoP F- (W3110 typeA←ΔphoP) ΔphoP W3110, W3110 type A LB medium 59088 ME9906 W3110ΔkdpE F- (W3110 typeA←ΔkdpE) W3110, ΔkdpE W3110 type A LB medium 59088 ME9907 W3110ΔcusR F- (W3110 typeA←ΔcusR) ΔcusR W3110, W3110 type A LB medium 59088 ME9908 W3110ΔrstA F- (W3110 typeA←ΔrstA) W3110, ΔrstA W3110 type A LB medium 59088 ME9909 W3110ΔhprR F- (W3110 typeA←ΔhprR) W3110, ΔhprR W3110 type A LB medium 59088 ME9910 W3110ΔcreB F- W3110, (W3110 typeA←ΔcreB) ΔcreB W3110 type A LB medium 59088 ME9911 W3110ΔarcA F- (W3110 typeA←ΔarcA) W3110, ΔarcA W3110 type A LB medium 59088 ME9912 W3110ΔbasR F- (W3110 typeA←ΔbasR) W3110, ΔbasR W3110 type A LB medium 59088 ME9913 W3110ΔbaeR F- (W3110 typeA←ΔbaeR) ΔbaeR W3110, W3110 type A LB medium 59088 ME9914 W3110ΔqseB F- W3110, ΔqseB (W3110 typeA←ΔqseB) W3110 type A LB medium 59088 ME9915 W3110ΔtorR F- ΔtorR (W3110 typeA←ΔtorR) W3110, W3110 type A LB medium 59088 ME9916 W3110ΔcpxR F- (W3110 typeA←ΔcpxR) ΔcpxR W3110, W3110 type A LB medium 59088 ME9917 W3110ΔompRΔphoB F- ΔompR, (W3110ΔphoB←ΔompR) ΔphoB W3110, W3110 type A LB medium 59088 ME9918 W3110ΔompRΔphoP F- ΔompR, W3110, (W3110ΔphoP←ΔompR) ΔphoP W3110 type A LB medium 59088 ME9919 W3110ΔphoBΔphoP F- ΔphoP W3110, ΔphoB, (W3110ΔphoB←ΔphoP) W3110 type A LB medium 59088 ME9920 W3110ΔompRΔphoBΔphoP F- ΔompR, ΔphoB, (W3110ΔphoBΔphoP←ΔompR) ΔphoP W3110, W3110 type A LB medium 59088 ME9921 W3110ΔompRΔenvZ F- ΔenvZ W3110, ΔompR, (W3110ΔompR←ΔenvZ) W3110 type A LB medium 59088 ME9922 W3110ΔphoBΔphoR F- (W3110ΔphoB←ΔphoR) W3110, ΔphoB, ΔphoR W3110 type A LB medium 59088 ME9923 W3110ΔphoPΔphoQ F- W3110, ΔphoQ (W3110ΔphoP←ΔphoQ) ΔphoP, W3110 type A LB medium 59088 ME9924 W3110ΔphoPΔkdpE F- ΔkdpE (W3110ΔphoP←ΔkdpE) ΔphoP, W3110, W3110 type A LB medium 59088 ME9925 W3110ΔphoBΔcreB F- W3110, ΔcreB ΔphoB, (W3110ΔphoB←ΔcreB) W3110 type A LB medium 59088 ME9926 W3110ΔompRΔcpxR F- W3110, (W3110ΔompR←ΔcpxR) ΔcpxR ΔompR, W3110 type A LB medium 59088 ME9927 W3110ΔompRΔrstA F- W3110, ΔompR, ΔrstA (W3110ΔompR←ΔrstA) W3110 type A LB medium 59088 ME9928 W3110ΔrstAΔcusRΔhprR F- ΔrstA, ΔcusR, ΔhprR W3110, W3110 type A LB medium 59088 ME9929 W3110ΔenvZ F- W3110, (W3110 typeA←ΔenvZ) ΔenvZ W3110 type A LB medium 59088 ME9930 W3110ΔphoR F- (W3110 typeA←ΔphoR) W3110, ΔphoR W3110 type A LB medium 59088 ME9931 W3110ΔphoQ F- ΔphoQ (W3110 typeA←ΔphoQ) W3110, W3110 type A LB medium 59088 ME9932 W3110ΔenvZΔphoR F- W3110, ΔphoR ΔenvZ, (W3110ΔphoR←ΔenvZ) W3110 type A LB medium 59088 ME9933 W3110ΔenvZΔphoQ F- W3110, ΔenvZ, ΔphoQ (W3110ΔphoQ←ΔenvZ) W3110 type A LB medium 59088 ME9934 W3110ΔphoRΔphoQ F- (W3110ΔphoQ←ΔphoR) ΔphoQ ΔphoR, W3110, W3110 type A LB medium 59088 ME9935 W3110ΔenvZΔphoRΔphoQ F- ΔphoR, ΔphoQ ΔenvZ, W3110, (W3110ΔphoQΔphoR←ΔenvZ) W3110 type A LB medium 59088 ME9939 W3110ΔatpD F- of W3110, substitution a to codon Lue 4th TAA with atpD defective W3110 type A LB medium 74726 ME9940 W3110ΔatpE F- of a with atpE defective W3110, substitution 2nd Ala codon to TAA W3110 type A LB medium 74726 ME9941 W3110ΔfimZ F- with to codon Phe fimZ defective TAA W3110, 13th of substitution a W3110 type A LB medium 74726 ME9942 W3110ΔphoBΔatpD F- TAA W3110ΔphoB, defective atpD with a substitution of 4th Lue codon to W3110 type A LB medium 74726 ME9943 W3110ΔphoBΔatpE F- a TAA to codon Ala 2nd with atpE defective W3110ΔphoB, of substitution W3110 type A LB medium 74726 ME9944 W3110ΔphoBΔfimZ F- codon W3110ΔphoB, defective fimZ with a substitution of 13th Phe to TAA W3110 type A LB medium 74726 ME9945 W3110ΔphoPΔatpD F- W3110ΔphoP, TAA to codon Lue 4th of substitution a with atpD defective W3110 type A LB medium 74726 ME9946 W3110ΔphoPΔatpE F- Ala W3110ΔphoP, defective atpE with a substitution of 2nd codon to TAA W3110 type A LB medium 74726 ME9947 W3110ΔphoPΔfimZ F- W3110ΔphoP, TAA to codon Phe 13th of substitution a with fimZ defective W3110 type A LB medium 74726 ME9948 W3110ΔphoBΔphoPΔatpD F- TAA W3110ΔphoBΔphoP, defective atpD with a substitution of 4th Lue codon to W3110 type A LB medium 74726 ME9949 W3110ΔphoBΔphoPΔatpE F- W3110ΔphoBΔphoP, defective atpE with a substitution of 2nd Ala TAA codon to W3110 type A LB medium 74726 ME9950 W3110ΔphoBΔphoPΔfimZ F- to TAA codon Phe 13th of substitution a with fimZ defective W3110ΔphoBΔphoP, W3110 type A LB medium 74726 ME10027 YG1012 As TA1538/1,8-DNP but has pYG213; acetyltransferase overproducer Ap M. Yamada and T. Nohmi Salmonella typhimurium 76858 ME10028 YG1021 As TA98 but has pYG216; nitroreductase overproducer Ap, Tc M. Yamada and T. Nohmi Salmonella typhimurium 76857 ME10029 YG1024 As TA98 but has pYG219; acetyltransferase overproducer Ap, Tc M. Yamada and T. Nohmi Salmonella typhimurium 76856 ME10030 YG1026 As TA100 but has pYG216; nitroreductase overproducer Ap, Tc M. Yamada and T. Nohmi Salmonella typhimurium 76857 ME10031 YG1029 As TA100 but has pYG219; acetyltransferase overproducer Ap, Tc M. Yamada and T. Nohmi Salmonella typhimurium 76856 ME10032 YG1041 As TA98 but has pYG233; nitroreductase and acetyltransferase overproducer Ap, Km M. Yamada and T. Nohmi Salmonella typhimurium 76859 ME10033 YG1042 As TA100 but has pYG233; nitroreductase and acetyltransferase overproducer Ap, Km M. Yamada and T. Nohmi Salmonella typhimurium 76859 ME10034 YG2975 As TA1975 but has pKM101 Ap M. Yamada and T. Nohmi Salmonella typhimurium 76855 ME10035 YG3001 As TA1535 but is deficient in mutMST Km M. Yamada and T. Nohmi Salmonella typhimurium 76863 ME10036 YG3002 As TA1975 but is deficient in mutMST Km M. Yamada and T. Nohmi Salmonella typhimurium 76863 ME10037 YG3003 As TA102 but is deficient in mutMST Ap, Km, Tc M. Yamada and T. Nohmi Salmonella typhimurium 76863 ME10038 YG3008 As TA100 but is deficient in mutMST Ap, Km M. Yamada and T. Nohmi Salmonella typhimurium 76943 ME10039 YG3021 As TA1535 but is deficient in mutMST, mutYST Cm, Km M. Yamada and T. Nohmi Salmonella typhimurium 76865 ME10040 YG3022 As TA1975 but is deficient in mutMST, mutYST Cm, Km M. Yamada and T. Nohmi Salmonella typhimurium 76865 ME10041 YG3201 As TA1535 but is deficient in neiST Cm M. Yamada and T. Nohmi Salmonella typhimurium 76943 ME10042 YG3203 As TA1535 but is deficient in nthST - M. Yamada and T. Nohmi Salmonella typhimurium 76943 ME10043 YG3206 As TA1535 but is deficient in neiST, nthST Cm M. Yamada and T. Nohmi Salmonella typhimurium 76943 ME10044 YG3216 As YG3206 but has pKM101 Ap, Cm M. Yamada and T. Nohmi Salmonella typhimurium 76943 ME10045 YG5120 As TA2659 but is deficient in umuDCST Km M. Yamada and T. Nohmi Salmonella typhimurium 76860 ME10046 YG5144 As TA2659 but is deficient in umuDCST and samAB Cm, Km M. Yamada and T. Nohmi Salmonella typhimurium 76860 ME10047 YG5145 As TA2659 but is deficient in samAB Cm M. Yamada and T. Nohmi Salmonella typhimurium 76860 ME10048 YG5147 As TA1538 but is ∆umuDCST::Kmr, ∆samAB::Cmr Cm, Km M. Yamada and T. Nohmi Salmonella typhimurium 76866 ME10049 YG5160 As TA1538 but has plasmid pYG787, which is a derivative of pWKS30 with the E. coli polB gene. Ap M. Yamada and T. Nohmi Salmonella typhimurium 76867 ME10050 YG5161 As TA1538 but has plasmid pYG768, which is a derivative of pWKS30 with the E. coli dinB gene. Ap M. Yamada and T. Nohmi Salmonella typhimurium 76867 ME10051 YG5185 As YG7158 but has pYG768, which is a derivative of pWKS30 with the E. coli dinB gene. Ap, Cm Km M. Yamada and T. Nohmi Salmonella typhimurium 76943 ME10052 YG6205 As TA1538 but is ∆dinBST::Spr Sp M. Yamada and T. Nohmi Salmonella typhimurium 76866 ME10053 YG6208 As TA1538 but is ∆polBST::Tcr Tc M. Yamada and T. Nohmi Salmonella typhimurium 76866 ME10054 YG6215 As TA1538 but is ∆umuDCST::Kmr, ∆samAB::Cmr, ∆dinBST::Spr, ∆polBST::Tcr Cm, Km, Sp, Tc M. Yamada and T. Nohmi Salmonella typhimurium 76866 ME10055 YG7100 As TA1535 but is deficient in adaST Km M. Yamada and T. Nohmi Salmonella typhimurium 76944 ME10056 YG7104 As TA1535 but is deficient in ogtST Cm M. Yamada and T. Nohmi Salmonella typhimurium 76861 ME10057 YG7108 As TA1535 but is deficient in ogtST adaST Cm, Km M. Yamada and T. Nohmi Salmonella typhimurium 76861 ME10058 YG7125 As TA1535 but is deficient in acetyltransferase gene Cm M. Yamada and T. Nohmi Salmonella typhimurium 76864 ME10059 YG7126 As TA100 but is deficient in acetyltransferase gene Ap, Cm M. Yamada and T. Nohmi Salmonella typhimurium 76864 ME10060 YG7127 As TA1535 but is deficient in nitroreductase gene Km M. Yamada and T. Nohmi Salmonella typhimurium 76862 ME10061 YG7128 As TA100 but is deficient in nitroreductase gene Ap, Km M. Yamada and T. Nohmi Salmonella typhimurium 76862 ME10062 YG7129 As TA1538 but is deficient in acetyltransferase gene (oat) Cm M. Yamada and T. Nohmi Salmonella typhimurium 76864 ME10063 YG7130 As TA98 but is deficient in acetyltransferase gene Ap, Cm M. Yamada and T. Nohmi Salmonella typhimurium 76864 ME10064 YG7131 As TA1538 but is deficient in nitroreductase gene Km M. Yamada and T. Nohmi Salmonella typhimurium 76862 ME10065 YG7132 As TA98 but is deficient in nitroreductase gene Ap, Km M. Yamada and T. Nohmi Salmonella typhimurium 76862 ME10066 YG7158 As TA1538 but deficient in acetyltransferase gene (oat) and nitroreductase gene (nfsB) Cm, Km M. Yamada and T. Nohmi Salmonella typhimurium 76943 ME10067 YG7167 As TA1537 but is deficient in nitroreductase gene Km M. Yamada and T. Nohmi Salmonella typhimurium 76868 ME10068 YG7168 As TA102 but is deficient in nitroreductase gene Ap, Km, Tc M. Yamada and T. Nohmi Salmonella typhimurium 76868 ME10069 TO114 F- nhaB::Emr chaA::Cmr nhaA::Kmr W3110 derivative kanamycin, chloramphenicol, erythromycin N. Uozumi, Tohoku Univ., January 2024 LB medium + 100mM KCl Phenotype: Na-sensitive 78086 ME10070 LB2003 F- metE rpsL ΔkdpABC5 aroE kup1 F- (trkD1) aroE+ ΔtrkA gal rha thi N. Uozumi, Tohoku Univ., January 2024 LB medium + 100mM KCl Phenotype: K-dependent growth 78087 ME10071 Δ4 (Δdghu) F- ΔkdpA Δkup ΔtrkH ΔtrkG BW25113 N. Uozumi, Tohoku Univ., January 2024 Phenotype: K-dependent growth 74360 ME10072 Δdhu (trkG+) F- Δkup Δkdp ΔtrkH BW25113 N. Uozumi, Tohoku Univ., January 2024 Phenotype: K-dependent growth 74360 ME10073 Δdgu (trkH+) F- Δkdp Δkup ΔtrkG BW25113 N. Uozumi, Tohoku Univ., January 2024 Phenotype: K-dependent growth 74360 ME10074 ΔtrkG ΔtrkH F- ΔtrkG ΔtrkH BW25113 N. Uozumi, Tohoku Univ., January 2024 74360 ME9951 KS26 F- ΔspeC::FRT-Cam-FRT rph-1 Δggt-2 ΔspeAB::FRT-kan-FRT ΔpotFGHI::FRT ΔpuuP::tet ΔydcSTUV::FRT ΔpotE::FRT ΔyeeF::FRT MG1655 Km, Cm, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 66225 ME9952 KS27 F- ΔpotE::FRT rph-1 Δggt-2 ΔspeAB::FRT-kan-FRT ΔspeC::FRT-Cam-FRT ΔpotABCD::FRT ΔpotFGHI::FRT ΔpuuP::tet ΔyeeF::FRT MG1655 Km, Cm, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 66225 ME9953 KS28 F- rph-1 ΔyeeF::FRT ΔydcSTUV::FRT ΔpuuP::tet ΔpotFGHI::FRT ΔpotABCD::FRT ΔspeC::FRT-Cam-FRT ΔspeAB::FRT-kan-FRT Δggt-2 MG1655 Km, Cm, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 66225 ME9954 KS29 F- Δggt-2 ΔpotE::FRT ΔyeeF::FRT ΔydcSTUV::FRT ΔpuuP::tet ΔpotABCD::FRT ΔspeC::FRT-Cm-FRT rph-1 ΔspeAB::FRT-kan-FRT MG1655 Km, Cm, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 66225 ME9955 SK549 F- rph-1 ΔpotE::FRT ΔpotFGHI::FRT ΔpuuP::Tet ΔydcSTUV::FRT ΔpotABCD::FRT ΔspeC::FRT-Cam-FRT ΔspeAB::FRT-kan-FRT Δggt-2 MG1655 Km, Cm, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 66225 ME9956 SK556 F- ΔpotABCD::FRT rph-1 Δggt-2 ΔspeAB::FRT-kan-FRT ΔspeC::FRT-Cam-FRT ΔydcSTUV::FRT ΔpotFGHI::FRT ΔpotE::FRT ΔyeeF::FRT MG1655 Km, Cm H. Suzuki, Kyoto Institute of Technology, March 2023 66225 ME9957 SK557 F- ΔpuuP::Tet Δggt-2 rph-1 ΔpotABCD::FRT ΔspeC::FRT ΔspeAB::FRT-kan-FRT ΔyeeF::FRT-Cam-FRT ΔpotE::FRT ΔpotFGHI::FRT ΔydcSTUV::FRT MG1655 Km, Cm, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 66225 ME9958 SO23 F- rph-1 Δggt-2 ΔpuuP::tet MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80717 ME9959 SK421 F- ΔydcSTUV::FRT ΔpotABCD::FRT rph-1 Δggt-2 ΔpotE::FRT ΔpotFGHI::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80717 ME9960 SK422 F- ΔpuuP::tet rph-1 Δggt-2 ΔpotABCD::FRT ΔpotE::FRT ΔydcSTUV::FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80717 ME9961 SK423 F- ΔpotFGHI::FRT ΔpuuP::tet ΔydcSTUV::FRT rph-1 Δggt-2 ΔpotE::FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80717 ME9962 SK424 F- ΔpotE::FRT ΔpotFGHI::FRT ΔpotABCD::FRT Δggt-2 rph-1 ΔpuuP::tet MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80717 ME9963 SK365 F- ΔydcSTUV::FRT ΔpotABCD::FRT ΔpuuP::tet Δggt-2 rph-1 ΔpotFGHI::FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80717 ME9964 SK425 F- Δggt-2 rph-1 ΔpotABCD::FRT ΔpotE::FRT ΔpotFGHI::FRT ΔpuuP::tet ΔydcSTUV::FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80717 ME9965 MR37 F- ΔspeF::FRT ΔspeAB::FRT Δggt-2 rph-1 ΔspeC::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 putrescine biosynthesis-deficient 66225 ME9966 MR40 F- Δggt-2 rph-1 ΔspeF::FRT ΔspeG::FRT ΔspeC::FRT ΔspeAB::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 putrescine biosynthesis-deficient 66225 ME9967 MR42 F- Δggt-2 rph-1 ΔspeF::FRT ΔspeG::FRT Δgss::Kan-FRT ΔspeC::FRT ΔspeAB::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 putrescine biosynthesis-deficient 66225 ME9968 SH1837 F- rph-1 Δggt-2 ΔspeAB-FRT ΔspeC-FRT ΔspeF-FRT Δgss::kan-FRT MG1655 Km H. Suzuki, Kyoto Institute of Technology, March 2023 putrescine biosynthesis-deficient 66225 ME9969 MR90 F- ΔspeF::FRT ΔspeC::FRT ΔspeAB::FRT Δggt-2 rph-1 ΔcadA::FRT ΔldcC::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 putrescine biosynthesis-deficient
cadaverine biosynthesis-deficient 66225 ME9970 MR92 F- ΔspeAB::FRT Δggt-2 ΔldcC::FRT ΔcadA::FRT ΔspeC::FRT ΔspeF::FRT ΔpotE::FRT-kan-FRT rph-1 MG1655 Km H. Suzuki, Kyoto Institute of Technology, March 2023 putrescine biosynthesis-deficient
cadaverine biosynthesis-deficient
spermidine biosynthesis-deficient 66225 ME9971 SH2166 F- ΔspeF::FRT rph-1 Δggt-2 ΔspeAB::FRT ΔpotD::FRT-kan-FRT ΔcadA::FRT ΔldcC::FRT ΔspeC::FRT MG1655 Km H. Suzuki, Kyoto Institute of Technology, March 2023 putrescine biosynthesis-deficient
cadaverine biosynthesis-deficient 66225 ME9972 SH1990 F- ΔcadA-FRT rph-1 Δggt-2 ΔspeAB-FRT ΔspeC-FRT ΔpatA-FRT ΔldcC-FRT ΔspeF-FRT Δpuu(PA-DR)::tet ΔspeE-FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 putrescine biosynthesis-deficient
cadaverine biosynthesis-deficient
spermidine biosynthesis-deficient ME9973 SH1994 F- ΔspeAB-FRT ΔspeF-FRT Δpuu(PA-DR)::tet ΔspeE-FRT ΔadiA::FRT-kan-FRT ΔpatA-FRT ΔspeC-FRT ΔcadA-FRT ΔldcC-FRT Δggt-2 rph-1 MG1655 Km, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 putrescine biosynthesis-deficient
cadaverine biosynthesis-deficient
spermidine biosynthesis-deficient ME9974 SH2179 F- ΔpatA-FRT ΔargA:::FRT-cam-FRT rph-1 Δggt-2 ΔspeAB-FRT ΔldcC-FRT ΔspeC-FRT ΔcadA-FRT ΔspeF-FRT Δpuu(PA-DR)::tet ΔspeE-FRT ΔadiA::kan-FRT MG1655 Km, Cm, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 putrescine biosynthesis-deficient
cadaverine biosynthesis-deficient
spermidine biosynthesis-deficient ME9975 SK123 F- ΔpuuD::FRT Δggt-2 rph-1 MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 This strain cannot hydrolyze γ-glutamyl-p-nitroanilide 80721 ME9976 SH639 F- rph-1 Δggt-2 MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 This strain cannot hydrolyze γ-glutamyl-p-nitroanilide ME9977 SH641 F- strR srl300::Tn10 rph-1 Δggt-2 recA56 MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 This strain cannot hydrolyze γ-glutamyl-p-nitroanilide ME9978 SH703 F- Δggt-2 rph-1 htp+-Tn10 MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 This strain cannot hydrolyze γ-glutamyl-p-nitroanilide ME9979 SH1967 F- ΔargA::FRT rph-1 Δggt-2 ΔadiA::FRT ΔspeAB::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 agmatine biosynthesis-deficient strain 74412 ME9980 SH2204 F- ΔspeG::FRT ΔargA::FRT ΔpuuPA::FRT zie-296::Tn10 rph-1 Δggt-2 yifE(Q100TAG) ΔargR::FRT ΔpatA::FRT ΔpotE::FRT ΔspeD::FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 70557 ME9981 SH2134 F- rph-1 Δ(ackA-pta)::FRT-camR-FRT ΔldhA::FRT-kanR-FRT ΔadhE::FRT ΔsdhA::FRT ΔiclR::FRT Δggt-2 MG1655 Km, Cm H. Suzuki, Kyoto Institute of Technology, March 2023 This strain accumulates succinate. 66185 ME9982 YG74 F- ΔpheP rph-1 ΔaroP ΔlivHMGF MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80713 ME9983 YG106 F- rph-1 ΔbrnQ ΔpheP ΔaroP MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80713 ME9984 YG195 F- ΔbrnQ ΔtyrP ΔaroP rph-1 Δmtr Δtna ΔpheP MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80713 ME9985 YG198 F- ΔlivHMGF ΔbrnQ rph-1 ΔaroP ΔtyrP Δmtr Δtna ΔpheP MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80713 ME9986 YG201 F- rph-1 ΔlivHMGF ΔbrnQ ΔpheP ΔaroP MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80713 ME9987 YG228 F- ΔbrnQ ΔpheP ΔaroP rph-1 Δ(livJ-yhhk-livKHMGF) MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80714 ME9988 YG299 F- Δ(livJ-yhhK-livKHMGF)::FRT-kan-FRT rph-1 MG1655 Km H. Suzuki, Kyoto Institute of Technology, March 2023 80714 ME9989 YG301 F- rph-1 Δ(livJ-yhhK-livKHMGF)::FRT-kan-FRT ΔaroP MG1655 Km H. Suzuki, Kyoto Institute of Technology, March 2023 80714 ME9990 KES2 F- rpsL pepB1 pepA11 pepN102 thyA met Δ(pro-lac) leu-9 CM86 H. Suzuki, Kyoto Institute of Technology, March 2023 80712 ME9991 KES22 F- rspL leu-9 Δ(pro-lac) met thyA pepN102 pepB1 cycA30::Tn10 CM86 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80712 ME9992 KES11 F- thyA met leu-9 Δ(pro-lac) pepN102 pepA11 pepB1 zff-208::Tn10 CM86 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80712 ME9993 KES17 F- met zae-502::Tn10 rpsL pepB1 pepA11 pepN102 thyA leu-9 CM86 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80712 ME9994 KES13 F- pepB1 pepA11 pepN102 thyA leu-9 Δ(pro-lac) met zcb-3059::Tn10 CM86 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80712 ME9995 SI28 F- rph-1Δggt-2 htp+-Tn10 ΔgsiAB::FRT-kan-FRT MG1655 Km, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80715 ME9996 SI101 F- rph-1 ΔgsiAB::FRT-kan-FRT htp+-Tn10 MG1655 Km, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80715 ME9997 KEI06 F- rph-1Δggt-2 ΔyneI::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80718 ME9998 KEI08 F- ΔgabD::FRT rph-1Δggt-2 MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80718 ME9999 KEI11 F- rph-1Δggt-2 ΔaldA::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80718 ME10000 KEI17 F- ΔyneI::FRT rph-1Δggt-2 ΔaldA::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80718 ME10001 KEI18 F- rph-1Δggt-2 ΔgabD::FRT ΔyneI::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80718 ME10002 KEI19 F- rph-1Δggt-2 ΔgabD::FRT ΔaldA::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80718 ME10003 KJ107 F- ΔgabT::FRT rph-1Δggt-2 MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80718 ME10004 SK187 F- rph-1Δggt-2 ΔpuuE::kan MG1655 Km H. Suzuki, Kyoto Institute of Technology, March 2023 80718 ME10005 KJ109 F- rph-1Δggt-2 ΔpuuE::FRT-kan-FRT ΔgabT::FRT MG1655 Km H. Suzuki, Kyoto Institute of Technology, March 2023 80718 ME10006 SK247 F- ΔpuuDR::FRT ΔpuuCBE::FRT rph-1 MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80716 ME10007 SO30 F- Δggt-2 rph-1 Δ(puuB-puuE)::kan ΔpuuPADR::tet MG1655 Km, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 ME10008 SK245 F- rph-1Δggt-2 ΔpuuCBE::FRT-kan-FRT ΔpuuDR::FRT-cam-FRT MG1655 Km, Cm H. Suzuki, Kyoto Institute of Technology, March 2023 ME10009 SO58 F- rph-1Δggt-2 ΔpuuADR::(FRT-cam-FRT) ΔpuuCBE::(FRT-kan-FRT) MG1655 Km, Cm H. Suzuki, Kyoto Institute of Technology, March 2023 ME10010 SK251 F- ΔpuuPADR::tet rph-1Δggt-2 ΔpuuCBE::(FRT-kan-FRT) MG1655 Km, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 ME10011 SK252 F- rph-1Δggt-2 ΔpuuPADR::tet ΔpuuCBE::FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 ME10012 SK430 F- ΔspeC::FRT ΔspeAB::FRT rph-1Δggt-2 ΔpotABCD::FRT-Cam-FRT MG1655 Cm H. Suzuki, Kyoto Institute of Technology, March 2023 80719 ME10013 SK468 F- ΔspeAB::FRT rph-1 Δggt-2 ΔpotABCD::FRT-Cam-FRT ΔspeC::FRT ΔpotE::FRT-Kan-FRT MG1655 Km, Cm H. Suzuki, Kyoto Institute of Technology, March 2023 80719 ME10014 YT21 F- ΔspeC::FRT ΔpotFGHI::FRT-kanR-FRT ΔpotABCD::FRT-Cam-FRT rph-1 Δggt-2 ΔspeAB::FRT MG1655 Km, Cm H. Suzuki, Kyoto Institute of Technology, March 2023 80719 ME10015 SK469 F- ΔspeAB::FRT ΔpotABCD::FRT-Cam-FRT ΔpuuP::Tet Δggt-2 rph-1 ΔspeC::FRT MG1655 Cm, Tet H. Suzuki, Kyoto Institute of Technology, March 2023 80719 ME10016 SK454 F- ΔpotABCD::FRT-Cam-FRT ΔspeC::FRT ΔspeAB::FRT Δggt-2 rph-1 ΔydcSTUV::FRT-Kan-FRT MG1655 Km, Cm H. Suzuki, Kyoto Institute of Technology, March 2023 80719 ME10017 SK505 F- ΔspeAB::FRT ΔplaP::FRT-Cam-FRT ΔpotABCD::FRT ΔspeC::FRT Δggt-2 rph-1 MG1655 Cm H. Suzuki, Kyoto Institute of Technology, March 2023 80719 ME10018 SK582 F- ΔplaP::FRT ΔydcSTUV::FRT ΔpotABCD::FRT ΔspeC::FRT ΔspeAB::FRT Δggt-2 rph-1 MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 80719 ME10019 SO23 F- Δggt-2 ΔpuuP::tet rph-1 MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 ME10020 SK421 F- rph-1 ΔpotFGHI::FRT ΔydcSTUV::FRT ΔpotE::FRT Δggt-2 ΔpotABCD::FRT MG1655 H. Suzuki, Kyoto Institute of Technology, March 2023 ME10021 SK422 F- ΔpuuP::tet rph-1 Δggt-2 ΔpotABCD::FRT ΔydcSTUV::FRT ΔpotE::FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 ME10022 SK423 F- ΔpotE::FRT rph-1 Δggt-2 ΔydcSTUV::FRT ΔpuuP::tet ΔpotFGHI::FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 ME10023 SK424 F- ΔpuuP::tet ΔpotFGHI::FRT rph-1 ΔpotE::FRT ΔpotABCD::FRT Δggt-2 MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 ME10024 SK365 F- ΔpuuP::tet+ ΔpotFGHI::FRT ΔydcSTUV::FRT rph-1 Δggt-2 ΔpotABCD::FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 ME10025 SK425 F- ΔpuuP::tet ΔpotE::FRT ΔpotFGHI::FRT Δggt-2 rph-1 ΔydcSTUV::FR ΔpotABCD::FRT MG1655 Tet H. Suzuki, Kyoto Institute of Technology, March 2023 ME10026 KEI12 F- puuPADR(3Ala)CBE+ pspF+ cat+ rph-1 kan+ ymjA+ MG1655 Km, Cm H. Suzuki, Kyoto Institute of Technology, March 2023 80720 ME10080 RLG7438 F- P1-parS ter at PMT-parS (Km), (Cm) oriC at VH1000 Cm, Km Richard L Gourse, University of Wisconsin, Apr 2024 81376 ME10081 RLG10650 F- at PMT-parS (Km), rrnE at P1-parS rrnB (Cm) VH1000 Cm, Km Richard L Gourse, University of Wisconsin, Apr 2024 81376 ME10082 RLG10654 F- rrnA (Km), PMT-parS at rrnH (Cm) P1-parS at VH1000 Cm, Km Richard L Gourse, University of Wisconsin, Apr 2024 81376 ME10083 RLG10669 F- (Km), (Cm) rrnE at PMT-parS rrnH at P1-parS VH1000 Cm, Km Richard L Gourse, University of Wisconsin, Apr 2024 81376 ME10084 RLG11975 F- at (Cm) rrnG P1-parS at rrnD (Km), PMT-parS VH1000 Cm, Km Richard L Gourse, University of Wisconsin, Apr 2024 81376 ME10085 RLG11980 F- (Cm) P1-parS at rrnG (Km), PMT-parS at rrnD VH1000 Cm, Km Richard L Gourse, University of Wisconsin, Apr 2024 81376 ME10086 RLG11999 F- P1-parS at rrnC (Km), PMT-parS at oriC (Cm) VH1000 Cm, Km Richard L Gourse, University of Wisconsin, Apr 2024 81376 ME10087 RLG12000 F- (Km), P1-parS at rrnC PMT-parS at rrnA (Cm) VH1000 Cm, Km Richard L Gourse, University of Wisconsin, Apr 2024 81376 ME10088 RLG13970 F- rrnG P1-parS at ΔrrnD (Cm) (Km), PMT-parS at VH1000 Cm, Km Richard L Gourse, University of Wisconsin, Apr 2024 81376 ME10089 RLG14083 F- P1-parS (Cm) rrnG at PMT-parS (Km), rrnDmut at VH1000 Cm, Km Richard L Gourse, University of Wisconsin, Apr 2024 81376 ME10090 RLG7474 F- VH1000 Amp Richard L Gourse, University of Wisconsin, Apr 2024 Amp 81376 ME10091 eCOMB F- Δ(yjjG-deoB) ΔthyA MG1655 Medium supplemented with thymidine (2 μg/ml or more) Recombinant status:non-recombinant
Phenotype:Requires thymidine
BrdU pulse labeling possible in 1-4 minutes (56/2 synthetic medium, 50 μg/ml BrdU) 85521 ME10092 MTA220 F- ΔendA Δ(yjjG-deoB) ΔthyA MG1655 Medium supplemented with thymidine (2 μg/ml or more) Recombinant status:non-recombinant
Phenotype:Requires thymidine
Hi-C analysis is possible with thymidine-requiring strains. ME10093 MTA274 F- Δ(yjjG-deoB) ΔthyA holD-TAP-kan MG1655 Km Medium supplemented with thymidine (2 μg/ml or more) Recombinant status:non-recombinant
Phenotype:Requires thymidine
DNA polymerase III-ChIP analysis is possible in thymidine-requiring strains.
References for holD-TAP-kan: Butland et al., Nature, 433, 531- 537, 2005. (DOI: 10.1038/nature03239)