遺伝子変異株 - 詳細

ME No. ME7946
菌株名 MC4100 rpoH165 [λpF13-(PrpoDhs-lacZ)]
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ λ+ λpF13-(PrpoDhs-lacZ)
遺伝子型 rpoH165(Amber) zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
親株(供与菌)
親株(受容菌) MC4100
変異導入方法 OTHER METHODSLysogenization
最終選別マーカー Prophage λpF13-(PrpoDhs-lacZ)
選別マーカー λ phage immunity
入手源
ゲノムマップ
文献 Yura T, Tobe T, Ito K, Osawa T.
Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.
Proc Natl Acad Sci U S A  (1984)  81(21)   6803-7  
[PubMed ID = 6387714]  [RRC文献情報] 
Casadaban MJ.
Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
J Mol Biol  (1976)  104(3)   541-55  
[PubMed ID = 781293]  [RRC文献情報] 
Yano R, Imai M, Yura T.
The use of operon fusions in studies of the heat-shock response: effects of altered sigma 32 on heat-shock promoter function in Escherichia coli.
Mol Gen Genet  (1987)  207(1)   24-8  
[PubMed ID = 3299002]  [RRC文献情報] 
Tobe T, Ito K, Yura T.
Isolation and physical mapping of temperature-sensitive mutants defective in heat-shock induction of proteins in Escherichia coli.
Mol Gen Genet  (1984)  195(1-2)   10-6  
[PubMed ID = 6092838]  [RRC文献情報] 
培地条件 L broth at 30C
その他備考 Unable to grow at high temperature (42C)
分譲依頼
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