遺伝子変異株 - 詳細

ME No. ME7938
菌株名 KY1431 Tets
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ
遺伝子型 rpoH15 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
親株(供与菌)
親株(受容菌) KY1431
変異導入方法 SPONTANEOUS PENICILLIN
最終選別マーカー Loss of Δzhf50::Tn10
選別マーカー Tetracycline sensitivity
入手源
ゲノムマップ
文献 Tobe T, Ito K, Yura T.
Isolation and physical mapping of temperature-sensitive mutants defective in heat-shock induction of proteins in Escherichia coli.
Mol Gen Genet  (1984)  195(1-2)   10-6  
[PubMed ID = 6092838]  [RRC文献情報
Yura T, Tobe T, Ito K, Osawa T.
Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.
Proc Natl Acad Sci U S A  (1984)  81(21)   6803-7  
[PubMed ID = 6387714]  [RRC文献情報
Casadaban MJ.
Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
J Mol Biol  (1976)  104(3)   541-55  
[PubMed ID = 781293]  [RRC文献情報
Yano R, Imai M, Yura T.
The use of operon fusions in studies of the heat-shock response: effects of altered sigma 32 on heat-shock promoter function in Escherichia coli.
Mol Gen Genet  (1987)  207(1)   24-8  
[PubMed ID = 3299002]  [RRC文献情報
培地条件 L broth at 30C
その他備考 Unable to grow at high temperature (42C)
分譲依頼
page-top
/ecoli/strain