遺伝子変異株 - 詳細

ME No. ME9276
菌株名 AQ3625
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ
遺伝子型 thr-1 leuB6 thi-1 lacY1 galK2 ara-4 xyl-5 mtl-1 proA2 his-60 argE3 rpsL31 tsx-33 supE44 recB21 recC22 sbcA23
親株(供与菌)
親株(受容菌)
変異導入方法
最終選別マーカー
選別マーカー
入手源 from A. J. Clark.
ゲノムマップ
文献 Asai T, Sommer S, Bailone A, Kogoma T.
Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.
EMBO J  (1993)  12(8)   3287-95  
[PubMed ID = 8344265]  [RRC文献情報
Tambosi R, Liotenberg S, Bourbon ML, Steunou AS, Babot M, Durand A, Kebaili N, Ouchane S.
Silver and Copper Acute Effects on Membrane Proteins and Impact on Photosynthetic and Respiratory Complexes in Bacteria.
mBio  (2018)  9(6)  
[PubMed ID = 30459190]  [RRC文献情報
Hasegawa H, Suzuki E, Maeda S.
Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms.
Front Microbiol  (2018)  9   2365  
[PubMed ID = 30337917]  [RRC文献情報
Sato R, Sawasato K, Nishiyama KI.
YnbB is a CdsA paralogue dedicated to biosynthesis of glycolipid MPIase involved in membrane protein integration.
Biochem Biophys Res Commun  (2019)  510(4)   636-642  
[PubMed ID = 30739787]  [RRC文献情報
培地条件 Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
その他備考 same as JC8679
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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