遺伝子変異株 - 詳細

ME No. ME9216
菌株名 AQ2200
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ
遺伝子型 thr-1 leuB6 proA2 his-4 argE3 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 rpsL31 tsx-33 supE4 recB21 recC22 sbcC15 recF143 bglR
親株(供与菌)
親株(受容菌)
変異導入方法
最終選別マーカー
選別マーカー
入手源 from A. J. Clark.
ゲノムマップ
文献 Torrey TA, Kogoma T.
Genetic analysis of constitutive stable DNA replication in rnh mutants of Escherichia coli K12.
Mol Gen Genet  (1987)  208(3)   420-7  
[PubMed ID = 2823060]  [RRC文献情報] 
培地条件 Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
その他備考 same as JC7539
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
分譲依頼
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