遺伝子変異株 - 詳細

ME No. ME9122
菌株名 AQ543
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ
遺伝子型 metE90 trpA9605 trpR55 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA56 srlC300::Tn10
親株(供与菌) JC10240
親株(受容菌) AQ377
変異導入方法 TD./PI
最終選別マーカー Rec-(30C), recA56
選別マーカー Tc-r
入手源
ゲノムマップ
文献 Torrey TA, Kogoma T.
Suppressor mutations (rin) that specifically suppress the recA+ dependence of stable DNA replication in Escherichia coliK-12.
Mol Gen Genet  (1982)  187(2)   225-30  
[PubMed ID = 6757658]  [RRC文献情報
培地条件 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
その他備考 Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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