Keio コレクション - Cell Shape
Cell Shape | Strains | |
---|---|---|
Normal | 3535 | |
Chain | 6 | |
Fat | 54 | |
Long | 153 | |
Long and Lysed | 1 | |
Long and Short | 2 | |
Round | 1 | |
Short | 57 | |
Lysed | 3 | |
Very long | 21 |
Procedures 1) Cells were inoculated in L broth containing Kan in 96 well plate overnight at 30℃. 2) Cells were diluted in L broth containing Kan and grown for 2 hours at 37℃. 3) Cells were harvested and resuspended. 4) Cells were spotted on poly-lysine coated slide glass,fixed in methanol and washed with H2O. 5) Cells were stained with DAPI and then observed. |