Gene Symbol Mnemonic Synonyms Gene Product Selectable Phenotype Culture Condition Growth Condition Teachers Comment Other Phenotypes Notes Reference
aarF aminoglycoside Ac-transferase regulator yigQR aminoglycoside acetyltransferase regulator unable to grow on succinate as a sole source of carbon (0.5 %) Luria-Bertani (LB) broth L broth Macinga, D. R. et al.
J. Bacteriol. 1998. 180: 128-135
aas acyl-ACP synthase 2-acyl-glycerophosphoethanolamine acyltransferase
acyl-acyl carrier protein synthetase unknown rich broth containing 10 g/liter tryptone, 1 g/liter yeast extract and 5 g/liter NaCl L broth Hsu, L. et al.
J. Biol Chem. 1991. 266: 13783-13788
aat amino acyl-tRNA-protein transferase aminoacyl-tRNA-protein-transferase exhibit a marked lag before resuming growth when grown into stationary phase and resuspend in minimal medium with glycerol enriched medium containing 0.8 % nutrient broth L broth Soffer, R. L. & M. Savage
Proc. Natl. Acad. Sci. USA. 1974. 71: 1004-1007
abrB (aidB regulatory) unknown minimal medium containing E salts, 0.4 % of glucose and 0.2 % of Bacto Casamino acids L broth Volkert, M. R. et al.
J. Bacteriol. 1994. 176: 7638-7645
accA accetyl-coA carboxylase acetyl-CoA carboxylase, alpha, carboxyltransferase subunit unknown (no mutant yet) L broth Kim, H. & S. K. Farrand
J. Biol. Chem. 1992. 267: 16841-16847
accB accetyl-coA carboxylase fabE acetyl-CoA carboxylase, biotin carboxy carrier protein sensitive to nonpermissive temperature (37 °C and 42 °C) 2 x YT medium (g/l; tryptone 16, yeast extract 10, NaCl 5) L broth Li, S.-J. & J. E. Cronan, Jr.
J. Biol. Chem. 1992. 267: 855-863
accC acetyl-CoA carboxylase fabG acetyl-CoA carboxylase, biotin carboxylase (BC) subunit no mutant yet Kondo, H. et al.
Proc. Natl. Acad. Sci. USA. 1991. 88: 9730-9733
accD acetyl-CoA carboxylase dedB usg acetyl-CoA carboxylase, beta, carboxyltransferase subunit sensitive to nonpermissive temperature (40 °C) medium 63 containing 1 % tryptone, 10 μg/ml yeast extract and 0.1 % Brij 58 L broth Harder, M. E. et al.
Proc. Natl. Acad. Sci. USA. 1972. 69: 3105-3109
aceA acetate icl isocitrate lyase unable to grow on acetate no description L broth Ashworth, J. M. & H. L. Kornberg
Biochim. Biophys. Acta. 1964. 89: 383-384
aceB acetate mas malate synthase A unknown Luria broth (Miller. 1972. J. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) L broth •in glcB-: unable to grow on acetate as a sole carbon source (50 mM) Maloy, S. R. & W. D. Nunn
J. Bacteriol. 1982. 149: 173-180
aceE acetate pyruvate dehydrogenase (decarboxylase component) E1p require acetate (2 mM) for growth on glucose (10 mM) or succinate (2 mM) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Langley, D. & J. R. Guest
J. Gen. Microbiol. 1977. 99: 263-276
aceF acetate aceE2 pyruvate dehydrogenase (dihydrolipoyltransacetylase component) E2p require acetate (2 mM) for growth on glucose (10 mM) or succinate (2 mM) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Langley, D. & J. R. Guest
J. Gen. Microbiol. 1977. 99: 263-276
aceK acetate isocitrate dehydrogenase kinase/phosphatase unable to grow on acetate (2 % sodium acetate) L broth containing 1 % Bacto-tryptone, 1 % NaCl and 0.5 % Bacto-yeast extract. L broth LaPorte, D. C. et al.
J. Biol. Chem. 1985. 260: 10563-10568
ackA acetate kinase acetate kinase grow on media containing 25 mM pyruvate or proline as sole carbon sources despite the presence of 10 or 50 mM sodium fluoroacetate defined liquid media containing 50 mM-sodium/potassium phosphate buffer pH 7.2, 50 mM-ammonium chloride, essential salts (4 micro-g of CaCl2.6H20; 8 mg of MgSO4.7H2O; 0.4 mig of MnSO4.4H2O; 0.4 mg FeSO4.7H2O per 100 ml of medium) and carbon source at 25 mM L broth •grow poorly on acetate (25 mM) as sole carbon source •in ackA- pta-:grow poorly on high concentrations(more than 25 mM) (J. Bacteriol. 1995. 177: 2878-2886)
•in acs- ackA- pta-unable to grow on acetate at any concentration tested (J. Bacteriol. 1995. 177: 2878-2886) Brown, T. D. K. et al.
J. Gen. Microbiol. 1977. 102: 327-336
acnA aconitase aconitase increase the lag phase during grow th in peptone medium, decrease the lag phase in succinate minimal medium and grow slightly higher in acetate minimal medium L broth L broth Gruer, M. J. & J. R. Guest
Microbiology. 1994. 140: 2531-2541
acnB aconitase aconitase B unable to grow on acetate (40 mM) as the sole source of carbon L broth L broth Gruer, M. J. et al.
Microbiology. 1997. 143: 1837-1846
acpD (acyl-carrier-protein) (azoreductase) no mutant yet Nakanishi, M. et al.
J. Biol. Chem. 2001. 276: 46394-46399
acpP acyl-carrier-protein ACP (acyl carrier protein) no mutant yet Rawlings, M. & J. E. Cronan, Jr.
J. Biol. Chem. 1992. 267-5751
acpS acyl-carrier-protein dpj holo-[acyl carrier protein] synthase unable to grow on a low concentration of pantothenate (0.25 uM) but grow on a much higher level (25 uM) R broth containing (in grams/liter): tryptone, 10; glucose, 1; yeast extract, 1; and sodium chloride, 5 L broth supplemented with pantothenate (25 uM) Polacco, M. L. & J. E. Cronan, Jr.
J. Biol. Chem. 1981. 256: 5750-5754
acpT
acrA acridine Mb lir mbl mtcA sipB AcrAB efflux system sensitive to Novobiocin (5 μg/ml) LB broth (Luria, S. E. et. al. 1957. J. Bacteriol. 74: 461-476) L broth •sensitive to Acriflavine (5 μg/ml), sodium deoxycholate (8.9 mg/ml), sodium dodecylsulfate (250μg/ml), crystal violet (5 μg/ml), methylene blue (5 μg/ml), and nalidixic acid (2 μg/ml) Coleman, W. G. et al.
J. Bacteriol. 1979. 139: 899-910
acrB acridine acrE AcrAB efflux pump sensitive to acriflavine (10 μg/ml) L broth containing Bacto tryptone 1 %, yeast extract 0.5 %, NaCl 0.5 %, glucose 0.1 % L broth Nakamura, H. et al.
J. Bacteriol. 1978. 134: 1184-1187
acrC acridine transmembrane protein affecting acriflavin sensitivity sensitive to nonpermissive temperature (43 °C) broth media PGY (J. Bacteriol. 1965. 90: 8-14) or L (Virology. 1955. 1: 190-206) L broth •in acrA-: resistant to acriflavine (4 μg/ml) Nakamura, H.
J. Bacteriol. 1979. 139: 8-12
acrD acridine cytoplasmic membrane multidrug resistance protein sensitive to nonpermissive temperature (43 °C) broth media PGY (J. Bacteriol. 1965. 90: 8-14) or L (Virology. 1955. 1: 190-206) L broth •in acrA-: resistant to acriflavine (4 μg/ml) Nakamura, H.
J. Bacteriol. 1979. 139: 8-12
acrE acridine sensitive to crystal violet (10 μg/ml) LB medium L broth Klein, J. R. et al.
Mol. Gen. Genet. 1991. 230: 230-340
acrF acridine envD lipoprotein with signal peptide, envelope sensitive to crystal violet (10 μg/ml) LB medium L broth Klein, J. R. et al.
Mol. Gen. Genet. 1991. 230: 230-340
acrR acridine modulator of acrAB transcription unknown L broth L broth •in tolC-: sensitive to deoxycholate (30 ul of 5 % (wt/vol) solution per 0.64 cm diameter disc) and sodium dodecyl sulfate (30 ul of 10 % (wt/vol) solution per disc) Fralick, J. A.
J. Bacteriol. 1996. 178: 5803-5805
acs acetyl CoA synthetase acetyl CoA synthetase 2 grow poorly on low concentrations (10 mM or less) of acetate, tryptone broth containing 1% (wt/vol) tryptone and 0.5% (wt/vol) sodium chloride or Luria broth containing TB and 0.5% (wt/vol) yeast extract L broth •in acs- ackA- pta-:unable to grow on acetate at any concentration tested. Kumari, S. et al.
J. Bacteriol. 1995. 177: 2878-2886
add adenine deaminase adenine deaminase unknown basal salt medium supplemented with carbohydrates (0.2 %), deoxyadenosine (4 μg/ml), and hypoxanthine (15 μg/ml) L broth •in purB-: grow on deoxyadenosine (4 μg/ml) and hypoxanthine (15 μg/ml) Jochimsen, B. et al.
Mol. Gen. Genet. 1975. 143: 85-91
adhC alcohol dehydrogenase alcohol-acetaldehyde dehydrogenase grow on ethanol (0.5 %) rich broth contained (per liter) tryptone (10 g), NaCl (5 g), and yeast extract (1 g) L broth •hypersensitive to chloroacetaldehyde (8 mg/l) Cunningham, P. R. & D. P. Clark
Mol. Gen. Genet. 1986. 205:487-493
adhE alcohol/acetald.dehydrogenase ana adhC alcohol dehydrogenase, acetaldehyde dehydrogenase, CoA-linked resistant to allyl alcohol (100 mM) anaerobically rich broth containing (per liter) tryptone (10 g), NaCl (5 g), and yeast extract (1 g) L broth •unable to grow aerobically in minimal media and sensitive to oxiditive stress (deletion mutant)(J. Biol. Chem. 2003. 278: 30193-30198) Lorowitz, W. & D. Clark
J. Bacteriol. 1982. 152: 935-938
adhP alcohol/acetald.dehydrogenase no mutant yet Shafqat, J. et al.
Eur. J. Biochem. 1999. 263: 305-311
adiA arginine decarboxylase, induced adi arginine decarboxylase, induced by acid turn Moeller arginine decarboxylase medium yellow LB medium containing 10 g of NaCl, 10 g of tryptone and 5 g of yeast extract per litre L broth Auger, E. A. et al.
Mol. Microbiol. 1989. 3: 609-620
adiY arginine decarboxylase, induced regulator of adiA no mutant yet Stim-Herndon, K. P. et al.
Microbiology 1996. 142: 1311-1320
adk adenylate kinase dnaW plsA adenylate kinase sensitive to nonpermissive temperature (42 °C) yeast-extract tryptone (YET) medium (Genetics. 1964. 49: 237-246) supplemented with 0.5 % NaCl L broth Henson, J. M. et al.
Mol. Gen. Genet. 1982. 186: 488-492
aegA anaerobically expressed gene yffG unknown Luria-Bertani (LB) broth L broth Cavicchioli, R. et al.
J. Bacteriol. 1996. 178: 6968-6974
aer fail to follow oxygien gradients in soft agar plates containing succinate (30 mM) tryptone medium L broth •fail to congregate around air bubbles Bibikov, S. I. et al.
J. Bacteriol. 1997. 179: 4075-4079
afuC no data
agaA acetylgalactosamine (deacetylase) unknown Lennox broth without glucose L broth Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agaB acetylgalactosamine putative N-acetylgalactosamine-specific IIb component 1, PTS no mutant yet Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agaC acetylgalactosamine putative N-acetylgalactosamine-specific IIC component 1, PTS no mutant yet Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agaD acetylgalactosamine putative N-acetylgalactosamine-specific IID component of PTS no mutant yet Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agaI acetylgalactosamine putative galactosamine-6-phosphate isomerase no mutant yet Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agaR acetylgalactosamine putative repressor aga operon no mutant yet Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agaS acetylgalactosamine (isomerase ?) no mutant yet Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agaV acetylgalactosamine putative N-acetylgalactosamine-specific IIb component 2 of PTS system no mutant yet Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agaW acetylgalactosamine putative N-acetylgalactosamine-specific IIc component 2, PTS system unknown Lennox broth without glucose L broth Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agaY acetylgalactosamine (aldose subunit I) no mutant yet Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agaZ acetylgalactosamine putative tagatose 6-phosphate kinase no mutant yet Brinkkotter, A. et al.
Mol. Microbiol. 2000. 37: 125-135
agp acid glucose-1-phosphatase periplasmic acid glucose-1-phosphatase no mutant yet Pradel, E. & P. L. Boquet
J. Bacteriol. 1988. 170: 4916-4923
ahpC alkyl hydroperoxide alkyl hydroperoxide reductase subunit resistant to organic solvents such as tetralin, propylbenzen and Cyclohexane Luria-Bertani broth containing 1.0 % tryptone, 0.5 % yeast extract and 1.0 % NaCl L broth Ferrante, A. A. et al.
Proc. Natl. Acad. Sci. USA. 1995. 92: 7617-7621
ahpF alkyl hydroperoxide alkyl hydroperoxide reductase subunit no mutant yet Ferrante, A. A. et al.
Proc. Natl. Acad. Sci. USA. 1995. 92: 7617-7621
aidB alkylating agent induced unknown L broth L broth Volkert, M. R. & D. C. Nguyen
Proc. Natl. Acad. Sci. USA. 1984. 81: 4110-4114
ais aluminum inducible protein aluminum inducible protein no data
alaS alanine act ala-act lovB alanyl-tRNA synthetase 1B (ligase) sensitive to nonpermissive temperature (40 °C) TGYE containing 1 % tryptone, 0.5 % yeast extract, and 0.2 % glucose L broth •in thyA-: resistant to thymineless death (J. Bacteriol. 1968. 95:991-997) Ruffler, D. et al.
Mol. Gen. Genet. 1974. 134: 313-323
alaV alanine alanine tRNA 1B unknown (no mutant yet) L broth Dorazi, R. et al.
Mol. Microbiol. 2002. 44: 131-141
aldA form pink (instead of red) colonies on MacConkey-L-fucose agar mineral medium (0.034 M NaH2PO4, 0.064 M K2HPO4, 0.02 M (NH4)2SO4, 3x10-4 M MgSO4, 10-6 M FeSO4, 10-6 M ZnC12, and 10-5 M CaC12.) with appropriate supplements and 10 mM D-xylose as the carbon and energy source L broth Zhu, Y. &. E. C. C. Lin
J. Bacteriol. 1987. 169: 785-789
aldB unknown Luria Bertani (LB) broth L broth Xu, J. & R. C. Johnson
J. Bacteriol 1995. 177: 938-947
aldH no mutant yet Heim, R. & E. E. Strehler
Gene. 1991. 99: 15-23
alkA sensitive to methyl methane sulfonate but not to UV light or to gamma rays nutrient broth L broth Yamamoto,Y. et al.
J. Bacteriol. 1978. 135: 144-152
alkB sensitive to methyl methane sulfonate but not to UV light (40 J/m2) LB medium L broth Kataoka, H. et al.
J. Bacteriol. 1983. 153: 1301-1307
allA unknown basal medium (containing 34 mM NaH2PO4, 64 mM K2HPO4, 20 mM (NH4)2SO4, 1 MM FeSO4, 0.1 mM MgSO4, and 10 ,uM CaCl2.) supplemented with a carbon source (60 mM) L broth Cusa, E. et al.
J. Bacteriol. 1999.181: 7479-7484
allB no mutant yet Cusa, E. et al.
J. Bacteriol. 1999.181: 7479-7484
allC no mutant yet Cusa, E. et al.
J. Bacteriol. 1999.181: 7479-7484
allD unable to grow on D-xylose as carbon source with allantoin (60 mM) used as nitrogen source basal medium (containing 34 mM NaH2PO4, 64 mM K2HPO4, 20 mM (NH4)2SO4, 1 MM FeSO4, 0.1 mM MgSO4, and 10 ,uM CaCl2.) supplemented with a carbon source (60 mM) L broth Rintoul, M. R. et al.
J. Mol. Biol. 2002. 324: 599-610
allR unknown basal medium (containing 34 mM NaH2PO4, 64 mM K2HPO4, 20 mM (NH4)2SO4, 1 MM FeSO4, 0.1 mM MgSO4, and 10 ,uM CaCl2.) supplemented with a carbon source (60 mM) L broth Cusa, E. et al.
J. Bacteriol. 1999.181: 7479-7484
alpA unknown LB L broth Trempy, J. E. et al.
J. Bacteriol. 1994. 176: 2061-2067
alr require either NaCl (2 %), sucrose (20 %) or D-alanine (40 μg/ml) for the growth at 42 °C nutrient broth containing 8 g Difco Nutrient broth and 5 g NaCl per liter Wijsman, H. J. W.
Genet. Res. 1972. 20: 269-277
amiA unknown LB medium •grows in long chains of unseparated cells Heidrich, C. et al.
Mol. Microbiol. 2001. 41: 167-178
amiB unknown LB medium •grows in long chains of unseparated cells Heidrich, C. et al.
Mol. Microbiol. 2001. 41: 167-178
amn AMP nucleosidase AMP nucleosidase unknown Luria broth L broth Leung, H. B. & V. L. Schramm
J. Biol. Chem. 1984. 259: 6972-6978
ampC ampicillin ampA beta-lactamase produce morphologically aberrant cells (deletion) Luria-Bertani medium (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) L broth •resistant to ampicillin (10 μg/ml) (J. Bacteriol. 1977. 132: 912-922 (duplication), J. Bacteriol. 1965. 99: 54-62 (increased expression)) Henderson, T. A. et al.
J. Bacteriol. 1997. 179: 6112-6121
ampD ampicillin regulartory gene, repressor for the ampC transcription resistant to cefotaxime (3 mg/ml) L broth medium of Bertani (J. Bacteriol. 1951. 62: 293-300) supplemented with medium E (J. Biol. Chem. 1956. 218: 97-106), 0.2 % glucose, and 1 mg/ml thiamine L broth Lindberg, F. et al.
J. Bacteriol. 1987. 169: 1923-1928
ampE ampicillin membrane protein mediating ampicillin sensitivity resistant to cefotaxime (50 μg/ml) L-broth medium of Bertani (Bertani, G. J. Bacteriol. 1951. 62: 293-300) L broth Lindquist, S. et al.
Mol. Microbiol. 1989. 3: 1091-1102
ampG ampicillin membrane protein mediating ampicillin sensitivity sensitive to ampicillin (256 μg/ml) in the presense of plasmid pNU305 L-broth medium L broth Lingquist, S. et al.
Mol. Microbiol. 1993. 9: 703-715
ampH ampicillin yaiH penicillin-binding protein, classC beta-lactamase-like unknown Luria-Bertani medium L broth Henderson, T. A. et al.
J. Bacteriol. 1997. 179: 6112-6121
amtB ammonia transport ammonium transport grow slowly at low NH4+ concentrations (1 mM or blow) only at pH values below 7 nitrogen-free, carbon-free medium (N-C- medium) containing (per liter) K2SO4 (1 g), K2HPO4•H2O (17.7 g), KH2PO4 (4.7 g), MgSO4 7H2O (0.1 g) and NaCl (2.5 g) supplemented with glucose as the carbon source (0.4 %) and NH4Cl as the nitrogen source (5 mM) L broth Soupene, E. et al.
Proc. Natl. Acad. Sci. 1998. 95: 7030-7034
amyA amylase alpha-amylase, cytoplasmic no mutant yet Raha, M. et al.
J. Bacteriol. 1992. 174: 6644-6652
ansA asparaginase L-asparaginase I grow on (NH4)2SO4 or sodium aspartate (0.01 mM) but not on asparagine (10 mM) as the sole nitrogen source Luria broth L broth Casale, T. D. et al.
J. Bacteriol. 1983. 154: 513-515
ansB asparaginase L-asparaginase II, periplasmic no mutant yet Jennings, M. P. & I. R. Beacham
J. Bacteriol. 1990. 172: 1491-1498
ansP no data
apbA no data
appA acid (poly)phosphate acid phosphate, pH 2.5, exopolyphosphotase unable to degrade paranitrophenyl phosphate (PNPP) at pH 2.5 MLT medium containing tryptone (10 g/l), yeast extract (5 g/l), and NaCl (5 g/l) L broth Dassa, E. & P. L. Boquet
Mol. Gen. Genet. 1985. 200: 68-73
appY acid(poly)phosphatase M5 polypeptide unknown Luria-Bertani medium L broth Atlung, T. & L. Bronsted
J. Bacteriol. 1994. 176: 5414-5422
aqpZ form smaller colonies Luria-Bertani medium L broth Calamita, G. et al.
Proc. Natl. Acad. Sci. USA. 1998. 95: 3627-3631
araA arabinose ara L-arabinose isomerase grow on glucose but not on L-arabinose(1 %) L-broth (Lennox, E. S. Virology. 1995. 1: 190-206) L broth •L-Arabinose inhibits growth on synthetic agar medium containing casein hydrolyzate as sole carbon and energy sources. Gross, J. & E. Englesberg
Virology. 1959. 9: 314-331
araB arabinose ara ribokinase grow on glucose but not on L-arabinose (1 %) L-broth (Lennox, E. S. Virology. 1995. 1: 190-206) L broth •L-Arabinose inhibits growth on synthetic agar medium containing casein hydrolyzate as sole carbon and energy sources. Gross, J. & E. Englesberg
Virology. 1959. 9: 314-331
araC arabinose regulatory gene grow on glucose but not on L-arabinose (1 %) L-broth (Lennox, E. S. Virology. 1995. 1: 190-206) L broth Gross, J. & E. Englesberg
Virology. 1959. 9: 314-331
araD arabinose ara L-ribulosephosphate 4-epimerase form minute pink colonies on EMB L-arabinose agar mineral, casein hydrolyzate (1.0 %), liquid medium L broth •L-Arabinose inhibits growth on synthetic agar medium containing any of following compounds as sole carbon and energy sources: casein hydrolyzate, D-fructose, D-mannose, D-xylose, D-arabinose, L-rhamnose, D-sorbitol, dulcitol, D-melibiose, glycerol, and DL-alanine. Englesberg, E. et al.
J. Bacteriol. 1962. 84: 137-146
araE arabinose grow substantially slower on minimal plate supplemented with arabinose (0.2 %, vol/vol) YT (Schleif, R., and P. Wensink. 1981. Practical methods in molecular biology. Springer-Verlag, New York.) L broth Reeder, T. & R. Schleif
J. Bacteriol. 1991. 173: 7765-7771
araJ arabinose unknown YT (Schleif, R., and P. Wensink. 1981. Practical methods in molecular biology. Springer-Verlag, New York.) L broth Reeder, T. & R. Schleif
J. Bacteriol. 1991. 173: 7765-7771
arcB Aerobic pathways control sensor component of 2-component ArcBA sensor-regulator system sensitive to toluidine blue (0.2 μg/ml) xylose-mineral medium (consisted of 0.034 M NaH2PO4, 0.064 M K2HPO4, 0.02 M (NH4)2SO4, 3x10-4 M MgSO4, 10-6 M FeSO4, 10-6 M ZnCl2, and 10-5 M CaC12) L broth Iuchi, S. et al.
J. Bacteriol. 1989. 171: 868-873
argA arginine Arg1 Arg2 argB N-acetylglutamate synthase require arginine (100 μg/ml) minimal medium supplemented with glucose (0.4 %) and arginine (100 μg/ml) L broth •grow on acetylornithine (25 μg/ml) Crabeel, M. et al.
J. Bacteriol. 1975. 123: 898-904
argB arginine acetylglutamate kinase require arginine (100 μg/ml) or ornithine liquid broth (869) containing Difco Bacto-tryptone 1 %, Difco yeast extract 0.5 %, glucose 0.1 %, and NaCl 0.5 % L broth Glansodorff, N.
Genetics. 1965. 51: 167-179
argC arginine Arg2 argH N-acetyl -gamma-glutamyl-phosphate require arginine (100 μg/ml) or ornithine liquid broth (869) containing Difco Bacto-tryptone 1 %, Difco yeast extract 0.5 %, glucose 0.1 %, and NaCl 0.5 % L broth Glansodorff, N.
Genetics. 1965. 51: 167-179
argD arginine ArgI argG acetylornithine aminotransferase require arginine (22 μg/ml) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth •in proA-, proB-: grow without proline Berg, C. M & J. J. Rossi
J. Bacteriol. 1974. 118: 928-939
argE arginine Arg4 argA acetylornithine deacetylase require arginine (100 μg/ml) or ornithine liquid broth (869) containing Difco Bacto-tryptone 1 %, Difco yeast extract 0.5 %, glucose 0.1 %, and NaCl 0.5 % L broth •resistant to acetylorvaline (25 mg/ml) (Mol. Gen. Genet. 1974. 132: 131-136 ) Glansodorff, N.
Genetics. 1965. 51: 167-179
argF arginine Arg5 argD ornithine transcarbamylase unknown (suppressed by argI) minimal medium supplemented with glucose (0.2 %) L broth •in argI-: grow on citrulline but not on ornithine, or require arginine (100 μg/ml) Glansodorff, N. et al.
Mutat. Res. 1967. 4: 743-751
argG arginine Arg6 argB arginosuccinate synthetase require arginine (100 μg/ml) liquid broth (869) containing Difco Bacto-tryptone 1 %, Difco yeast extract 0.5 %, glucose 0.1 %, and NaCl 0.5 % L broth Legrain, C. et al.
J. Bacteriol. 1976. 128: 39-48
argH arginine arginosuccinate lyase require arginine (100 μg/ml) liquid broth (869) containing Difco Bacto-tryptone 1 %, Difco yeast extract 0.5 %, glucose 0.1 %, and NaCl 0.5 % L broth Glansodorff, N.
Genetics. 1965. 51: 167-179
argI arginine ornithine transcarbamylase unknown (suppressed by argF) minimal medium supplemented with glucose (0.2 %) L broth •in argF-: grow on citrulline but not on ornithine, or require arginine (100 μg/ml) Glansodorff, N., G. Sand, and C. Verhoef
Mutat. Res. 4: 743-751 (1967)
argR arginine xerA repressor of Arg regulon resistant to indospicine (1000 μg/ml) Difco arginine assay medium L broth •resistant to glycylglycyl-N-delta-(phosphonoacetyl)-L-arabinose-ornithine (J. Biol. Chem. 1979. 254: 6392-6396)
•resistant to canavanine (100 μg/ml) + azauracil (100 μg/ml), resistant to canavanine (100 μg/ml) + 2-thiouracil (100 μg/ml)(Mol. Gen. Genet. 1972. 118: 235-245) Leisinger, T. et al.
Biochim. Biophys. Acta. 1972. 262: 214-219
argS arginine lov arginyl-tRNA synthetase resistant to canavanine (100 μg/ml) arginine-free medium (Novic, R. P. and W. K. Maas. J. Bacteriol. 81: 236- (1961)) Hirshifield, I. N. et al.
J. Mol. Biol. 1968. 35: 83-93
aroA aromatic 3-enolpyruvylshikimate-5-phosphate synthetase require tyrosine, phenylalanine and tryptophan (500 microM-2 mM each) L broth (Luria, S. E. et. al. J. Bacteriol. 1957. 74: 461-476) L broth Pittard, J. & B. J. Wallace
J. Bacteriol. 1966. 91: 1494-1508
aroB aromatic 3-Dehydroquinate synthase require shikimic acid (30 μg/ml) L broth (Luria, S. E. et. al. J. Bacteriol. 1957. 74: 461-476) L broth Pittard, J. & B. J. Wallace
J. Bacteriol. 1966. 91: 1494-1508
aroC aromatic Chorismate synthase require tyrosine, phenylalanine and tryptophan (500 microM-2 mM each), sometimes require tthese amino acids plus p-aminobenzoic acid and p-hydroxybenzoic acid L broth (Luria, S. E. et. al. J. Bacteriol. 1957. 74: 461-476) L broth Pittard, J. & B. J. Wallace
J. Bacteriol. 1966. 91: 1494-1508
aroD aromatic 3-dehydroquinate dehydratase require tyrosine, phenylalanine and tryptophan, or require shikimic acid (500 microM-2 mM each) L broth (Luria, S. E. et. al. J. Bacteriol. 1957. 74: 461-476) L broth Pittard, J. & B. J. Wallace
J. Bacteriol. 1966. 91: 1494-1508
aroE aromatic Dehydroshikimate reductase require shikimic acid, phenylalanine, and tyrosine, or require phenylalanine, tyrosine, tryptophan, p-aminobenzoic acid, and p-hydroxybenzoic acid (500 microM-2 mM each) L broth (Luria, S. E. et. al. J. Bacteriol. 1957. 74: 461-476) L broth •require phenylalanine, tyrosine, and tryptophan (Mol. Gen. Genet. 1975. 141: 207-211) Pittard, J. & B. J. Wallace
J. Bacteriol. 1966. 91: 1494-1508
aroH aromatic 3-deoxy-D-arabinoheptulosonate-7-phosphatesynthase unknown L-broth supplemented with 0.1 % glucose L broth •in aroF- aroG-: require tyrosine, phenylalanine and tryptophan Pittard, J. et al.
J. Bacteriol. 1969. 97: 1242-1247
aroP aromatic general aromatic amino acid transport; TyrR regulon resistant to beta-2-thienylalanine (10-4 M), DL-5-methyltryptophan (2x10-4 M), and DL-p-fluorophenylalanine (2x10-4 M) L broth of Luria and Burrous (J. Bacteriol. 1957. 74: 461-476) L broth Brown, K. D.
J. Bacteriol. 1970. 104: 177-188
aroT aromatic aroR resistant to indole acrylic acid (100 micro-l/ml) LC broth of Luria & Burrous (J. Bacteriol. 1957. 74: 461-476), Penassay broth, or nutrient broth (Difco) L broth Thorne, G. M. & L. M. Corwin
J. Gen. Microbiol. 1975. 90: 203-216
asd aspartate semialdehyde deHase dap hom aspartate semialdehyde dehydrogenase require diaminopimelic acid (0.01 %) even in complete medium ML medium supplemented with diaminopimelic acid (0.01 %) L broth supplemented with diaminopimelic acid (0.01 %) Thirion, J. P., and M. Hofnung
Genetics. 1972. 71: 207-216
asnA asparagine asparagine synthetase A unknown L broth or R broth (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth •in asnB-: require asparagine (100 μg/ml) Felton, J. et al.
J. Bacteriol. 1980. 142: 221-228
asnB asparagine asparagine synthetase B unknown L broth or R broth (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth •in asnA-: require asparagine (100 μg/ml) Felton, J. et al.
J. Bacteriol. 1980. 142: 221-228
aspA aspartate aspartate ammonia-lyase unable to grow on glutamate (0.5 %) as the sole source of carbon and energy (Biochim. Biophys. Acta. 1969. 177: 314-320) L or LG broth L broth Guest, J. R. et al.
J. Gen. Microbiol. 1984. 130: 1271-1278
aspC aspartate aspartate aminotransferase require aspartate (20 μg/ml) L-broth (pH 7.0-7.2) containing 1 % polypeptone, 0.5 % yeast sxtract, and 0.5 % NaCl L broth •unable to grow on glutamate (0.5 %) as the sole source of carbon and energy (Biochim. Biophys. Acta. 1969. 177: 314-320) •aspC13 mutants require 50 μg/ml of tyrosine and phenylalanine even in L broth. (J. Bacteriol. 1977. 130: 429-440) Kuramitsu, S. et al
J. Biochem. 1985. 97: 1259-1262
aspU aspartate aspartate tRNA 1 unknown (no mutant yet) Horiuchi, T. et al.
Mol. Gen. Genet. 1987. 206: 356-357
aspV aspartate aspartate tRNA unknown (no mutant yet) Horiuchi, T. et al.
Mol. Gen. Genet. 1987. 206: 356-357
asuE asparagine utilization temperature-sensitive (42 °C ) for growth on asparagine (5 mM) as the sole nitrogen source LB L broth Chesney, R. H. et al.
J. Gen. Microbiol. 1985. 131: 2079-2085
atoC acetoacetate butD positive rugulator in two-component system, with AtoS sensor kinase resistant to 4-pentenoate (10 mM), unable to grow on acetoacetate (10 mM) Luria broth or tryptone broth L broth Jenkins, L. S. & W. D. Nunn
J. Bacteriol. 1987. 169: 42-52
atpA ATP papA uncA membrane-bound ATP synthase, F1 sector, alpha-subunit unable to grow aerobically on a nonfermentable carbon source (e. g. succinate 0.4 %) LB containing 10 g of Difco tryptone, 5 g of Difco yeast extract, and 10 g of NaCl per liter L broth •unable to grow anaerobically even on rich medium (e. g. LB) containing glucose (3 mM) Scarpetta, M. A. et al.
J. Biol. Chem. 1991. 266: 18567-18572
avtA Alanine-isoketoValerate Transaminase alanine-alpha-ketoisovalerate transaminase unknown Lennox (L) complex medium L broth •in ilvE-: require isoleucine (0.15 mM) and valine (0.17 mM)
•in delta ilvGEDA: require isoleucine (0.15 mM), valine (0.17 mM), leucine (0.15 mM) and pantothenate (0.0021 mM) Whalen, W. A. & C. M. Berg
J. Bacteriol. 1982. 150: 739-746
be unknown unknown unknown Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y. L broth Ravi, R. S. et al.
Mol. Gen. Genet. 1985. 198: 390-392
bef unknown unknown unknown Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y. L broth Ravi, R. S. et al.
Mol. Gen. Genet. 1985. 198: 390-392
betA betaine choline dehydrogenase grow on high osmotic medium (including 0.8 M NaCl) supplemented with glycine betaine (1 mM) but not with choline (1 mM) medium 63 (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) with 22 mM glucose, 9 mM N-acetylglucosamine, or 1.7 mM sodiu citrate plus 11 mM lactose as the carbon source L broth Styrvold, O. B. et al.
J. Bacteriol. 1986. 165: 856-863
betB betaine betaine aldehyde dehydrogenase unknown (no mutant yet) medium 63 containing 100 mM KH2PO4 75 mM KOH, 15 mM (NH4)2SO4, 1mM MgSO4, 4 microM FeSO4 and 20 mM glucose L broth Andresen, P. A. et al.
J. Gen. Microbiol. 1988. 134: 1737-1746
betI betaine regulatory gene unknown (no mutant yet) medium 63 (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) supplemented with 20 mM glucose L broth Lamark, T. et al.
J. Bacteriol. 1996. 178: 1655-1662
betT betaine high-affinity choline transport unknown (no mutant yet) medium 63 containing 100 mM KH2PO4 75 mM KOH, 15 mM (NH4)2SO4, 1mM MgSO4, 4 microM FeSO4 and 20 mM glucose L broth Andresen, P. A. et al.
J. Gen. Microbiol. 1988. 134: 1737-1746
bglA beta-glucoside bglD phospho-beta-glucosidase A unknown medium A containing (per liter) 7 g of K2HPO4, 3 g of KH2PO4, and 0.1 g of (NH4)2SO4, supplemented with 0.2 % yeast extract (Difco) and 0.5 % succinate or LB L broth •nomenclature assigned previously or Newly (J. Bacteriol. 1974. 120: 638-650) Prasad, I. et. al
J. Bacteriol. 1973. 114: 909-915
bglB beta-glucoside bglA phospho-beta-glucosidase B grow on arbutin (0.5 %) medium A containing (per liter) 7 g of K2HPO4, 3 g of KH2PO4, and 0.1 g of (NH4)2SO4, supplemented with 0.2 % yeast extract (Difco) and 0.5 % succinate or LB L broth •nomenclature assigned previously or Newly (J. Bacteriol. 1974. 120: 638-650) Prasad, I. et al.
J. Bacteriol. 1973. 114: 909-915
bglG beta-glucoside bglC bglS positive regulatory RNA-binding protein unknown LB L broth •in bglR-: unable to grow on arbutin or salicin (0.5 % (w/v)) (same phenotype as that of wild type)
•nomenclature assigned previously or Newly (J. Bacteriol. 1974. 120: 638-650) Mahadevan, S. et al.
J. Bacteriol. 1987. 169: 2570-2578
bglR beta-glucoside regulatory gene of bgl operon grow on arbutin or salicin (0.5 % (w/v)) medium A containing (per liter) 7 g of K2HPO4, 3 g of KH2PO4, and 0.1 g of (NH4)2SO4, supplemented with 0.2 % yeast extract (Difco) and 0.5 % succinate L broth •form red colonies on MacConkey salicin indicator plate (Nature. 1981. 293: 625-629) •nomenclature assigned previously or Newly (J. Bacteriol. 1974. 120: 638-650) Prasad, I. & S. Schaefler
J. Bacteriol. 1974. 120: 638-650
bioA biotin diaminopelargonic acid synthetase require biotin (10-4 μg/ml) nutrient broth (Virology. 1967. 32: 553-569) L broth Rolfe, B. & M. A. Eisenberg
J. Bacteriol. 1968. 96: 515-524
bioB biotin biotin synthase require biotin (10-4 μg/ml) nutrient broth (Virology. 1967. 32: 553-569) L broth Rolfe, B. & M. A. Eisenberg
J. Bacteriol. 1968. 96: 515-524
bioC biotin blocked prior to pimeoloyl CoA formation require biotin (0.1 mg/l) tryptone broth L broth Cleary, P. P. & A. Campbell
J. Bacteriol. 1972. 112: 830-839
bioD biotin dethibiotin synthase require biotin (10-4 μg/ml) nutrient broth (Virology. 1967. 32: 553-569) L broth Rolfe, B. & M. A. Eisenberg
J. Bacteriol. 1968. 96: 515-524
bioF biotin 7-keto-8-aminopelargonic acid synthetase require biotin (10-4 μg/ml) nutrient broth (Virology. 1967. 32: 553-569) L broth Eisenberg, M. A. & C. Star
J. Bacteriol. 1968. 96: 1298-1297
bisC biotin sulfoxide biotin sulfoxde reductase unable to grow on D-biotin-d-sulfoxide (10-3 μg/ml) tryptone broth (Virology. 1961. 14: 22-32) L broth Dykhuizen, D.
J. Bacteriol. 1973. 115: 662-667
brnQ branched chain hrbA resistant to valine (100 μg/ml) in the presense of leucine (50 μg/ml) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth •sensitive to valine-containing dipeptide
•resistant to omicron-metylthreonine
(10 micro-l of a 5 mg/ml solution of the sabstance was pipetted onto a Whatman no.3 MM paper applied on the plate.) Guardiola, J. & M. Iaccarino
J. Bacteriol. 1971. 108: 1034-1044
brnR branched chain resistant to valine (50 micro-g/ml) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) or nutrient broth (5 g of NaCl and 8 g of nutrient broth [Difco] in 1 liter of distilled water) L broth • sensitive to glycylvaline (50 micro-g/ml) Guardiola, J. & M. Iaccarino
J. Bacteriol. 1974. 117: 393-405
btuB B12 uptake bfe, cer receptor for vitamin B12, E colicins, and phage BF23 resistant to BF23 or colicinE nutrient broth L broth Buxton, R. S.
Mol. Gen. Genet. 1971. 113: 154-156
can canavanine resistant to canavanine (100 μg/ml) minimal medium (Tanaka et. al. J. Bacteriol. 93: 642-648(1967)) containing 0.064 M NaH2PO4, 0.064 M K2HPO4, 0.02 M (NH4)2SO4, 3x10-4 MgSO4, 10-6 M FeSO4, 10-6 M ZnCl2, and 10-5 M CaCl2 (pH adjusted to 7.0 with HCl), supplemented with 0.4 % glycerol Rosen, B. P.
J. Bacteriol. 1973. 116: 627-635
carA carbamoyl P arg+ura cap pyrA carbamoylphosphate synthase (glutamine-hydrolysing) light subunit require arginine (100 μg/ml) and urasil (50 μg/ml) Liquid broth (869) containing 1 % Bacto-tryptone (Difco), 0.5 % yeast extract (Difco), 0.1 % glucose, 0.5 % NaCl L broth supplemented with urasil (50 μg/ml) Mergeary, M. et al.
Mol. Gen. Genet. 1974. 133: 299-316
carB carbamoyl P arg+ura cap pyrA carbamoylphosphate synthase (ammonia) heavy subunit require arginine (100 μg/ml) and urasil (50 μg/ml) Liquid broth (869) containing 1 % Bacto-tryptone (Difco), 0.5 % yeast extract (Difco), 0.1 % glucose, 0.5 % NaCl L broth supplemented with urasil (50 μg/ml) Mergeary, M. et al.
Mol. Gen. Genet. 1974. 133: 299-316
cdd deoxycytidine deaminase deoxycytidine deaminase resistant to 5-fluorodeoxycytidine (10 μg/ml) but sensitive to 5-fluorodeoxyuridine (5 μg/ml) in the presense of uracil glucose minimal medium (Biochim. Biophys. Acta. 1961. 80: 542-551) L broth •unable to grow on 0.04 % fructose (2.2 mM) or 0.44 % cytidine (18 mM) as the sole carbon source (J. Bacteriol. 1980. 144: 952-956) O'Donovan, G. A.et al.
J. Bacteriol. 1971. 105: 666-672
cdh CDP diglyceride hydrolase CDP-diglyceride hydrolase unknown LB (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) L broth Bulawa, C. E. & C. R. H. Raetz
J. Biol. Chem. 1984. 259: 11257-11264
cdsA CDP diglyceride synthase CDP diglyceride synthase unknown LB broth containing 10 g of NaCl, 10 g of Tryptone (Difco), and 5 g of yeast extract (Difco) per liter L broth Ganong, B. R. et al.
J. Biol. Chem. 1980. 255: 1623-1629
cirA colicin I resistance/receptor feuA colicin I receptor production resistant to colocin Ia LB L broth Boos, W. et al.
Mol. Gen. Genet. 1983. 191: 401-306
citA citrate cryptic gene for citrate transport system unknown L-broth (Miller. 1972. J. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) L broth •in citB-: grow on sodium citrate (0.42 g/l) as a source of carbon and energy Hall, B. G.
J. Bacteriol. 151: 1982. 269-273
citB citrate cryptic gene for citrate transport system unknown L-broth (Miller. 1972. J. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) L broth •in citA-: grow on sodium citrate (0.42 g/l) as a source of carbon and energy Hall, B. G.
J. Bacteriol. 1982. 151: 269-273
cld chain length determination wzzB wzz rol unknown (no mutant yet, mot studied in E. coli K-12) Batchelor, R. A. et al.
J. Bacteriol. 1991. 173: 5699-5704
cmlA chloramphenicol cmr? resistant to chloramphenicol (7 μg/ml) broth L broth Reeve, E. C. R.
Genet. Res. 1968. 11: 303-309
codA cytosine deaminase cytosine deaminase resistant to 5-fluorocytosine (20 μg/ml) but sensitive to fluorourasil (0.25 μg/ml) minimal medium L broth Ahmad, S. I. & R. H. Pritchard
Mol. Gen. Genet. 1972. 118: 323-325
codB cytosine deaminase cytosine permease unknown AB medium (J. Mol. Biol. 1967. 23: 99-112) supplemented with glucose (2 mg/ml) and thiamine (1 μg/ml) L broth •unable to utilize low concentration of cytosine (Genetika. 1973. 9: 116-121) Danielsen, S. et al.
Mol. Microbiol. 1992. 6: 1335-1344
cpsA capsular polysaccharide synthesis colanic acid (CPS) biosynthesis unknown LB L broth •suppress mucoidy of lon mutants (in lon-: form nonmucoid colonies) Trisler, P. & S. Gottesman
J. Bacteriol. 1984. 160: 184-191
cpsB capsular polysaccharide synthesis colanic acid (CPS) biosynthesis, mannose 1-P guanyltransferase unknown LB L broth •suppress mucoidy of lon mutants (in lon-: form nonmucoid colonies) Trisler, P. & S. Gottesman
J. Bacteriol. 1984. 160: 184-191
cpsC capsular polysaccharide synthesis colanic acid (CPS) biosynthesis unknown LB L broth •suppress mucoidy of lon mutants (in lon-: form nonmucoid colonies) Trisler, P. & S. Gottesman
J. Bacteriol. 1984. 160: 184-191
cpsD capsular polysaccharide synthesis colanic acid (CPS) biosynthesis unknown LB L broth •suppress mucoidy of lon mutants (in lon-: form nonmucoid colonies) Trisler, P. & S. Gottesman
J. Bacteriol. 1984. 160: 184-191
cpsE capsular polysaccharide synthesis colanic acid (CPS) biosynthesis unknown LB L broth •suppress mucoidy of lon mutants (in lon-: form nonmucoid colonies) Trisler, P. & S. Gottesman
J. Bacteriol. 1984. 160: 184-191
cpsF capsular polysaccharide synthesis colanic acid (CPS) biosynthesis unknown LB L broth •in lon-: form partially mucoid colonies Trisler, P. & S. Gottesman
J. Bacteriol. 1984. 160: 184-191
creC catabolite regulation phoM sensor in Pho regulon unknown LB L broth •in phoR-: form white colonies on plates containing 5-bromo-4-chloro-3-indolyl-phosphate-p-toluidine (2x10-3 %) and glucose (0.2 %) at high phosphate concentration (2x10-3 M) Wanner, B. L. & P. Latterell
Genetics. 1980. 96: 353-366
crl curli regulatory protein for curli unknown (no mutant yet) Olsen, A. et al.
Nature. 1989. 338: 652-655
crp cAMP receptor protein cAMP receptor protein resistant to 5-fluorouracil (2 μg/ml) plus 5'-AMP (1 mM) M9 medium (J. Mol. Biol. 1964. 9: 288-307) supplemented with glucose (0.2 %) L broth •unable to grow on AMP as carbon source
•resistant to phage lambda on arabinose + maltose indecator plates (J. Bacteriol. 1973. 116: 582-587)
•resistant to phage lambda and nalidixic acid (J. Bateriol. 1976. 125: 545-555) •in relA-: resistant to L-serine (Mol. Gen. Genet. 1979. 176: 343-350) Beacham, I. R. et al.
J. Bacteriol. 1973. 116: 957-964
csiB carbon-starvation induced stationary phase-induced protein unknown (no mutant yet) Weichart, D. et al.
Mol. Microbiol. 1993. 10: 407-420
cxm carbon-xylose metabolism cxr methyl glycerol synthesis grow on D-xylose and glycerol (both 5 g/l) in the presense of cAMP (2 mM) K115 medium containing 46 mM of K2HPO4, 23 mM of KH2PO4, 8 mM of (NH4)2SO4, 0.4 mM of MgSO4, 6 microM of FeSO4, 1 mM of sodium citratem 1 mg/l of thiamine hydrochloride, and 2 g/l of glycerol L broth Ackerman, R. S. et al.
J. Bacteriol. 1974. 119: 357-362
cyaA cyclase, adenylate adenylate cyclase resistant to phage lambda on arabinose + maltose indecator plates LB broth (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) L broth •resistant to phage lambda and nalidixic acid (J. Bateriol. 1976. 125: 545-555) •in relA-: resistant to L-serine (Mol. Gen. Genet. 1979. 176: 343-350) Brickman, E. et al.
J. Bacteriol. 1973. 116: 582-587
cycA cycloserine dagA D-alamine, D-serine, glycine permease resistant to C-cycloserin (1.2x10-4M) L broth (Luria, S.E. et. al. Virology. 1960. 12: 348-390) L broth Cosloy, S. D.
J. Bacteriol. 1973. 114: 679-684
cynR cyanase; cyanate metabolism unknown (no mutant yet) Sung, Y.-C. & J. A. Fuchs
J. Bacteriol. 1992. 174: 3645-3650
cynS cyanase; cyanate metabolism cnt cyanate aminohydrase sensitive to low concentrations of cyanate (<1 mM) in minimal A medium L broth L broth Guilloton, M. & F. Karst
J. Gen. Microbiol. 1987. 133: 645-653
cynT cyanase; cyanate metabolism carbonic anhydrase unable to grow at a significant rate with cyanate (0.5 mM) as the sole source of nitrogen at low pCO2 (aerated with air 0.3 % CO2) LB broth L broth •0.5 mM cyanate inhibits the growth in minimal glucose medium. Guilloton, M. B. et al.
J. Bacteriol. 1993. 175: 1443-1451
cynX cyanase; cyanate metabolism unknown LB broth L broth Guilloton, M. B. et al.
J. Bacteriol. 1993. 175: 1443-1451
cysB Cystein positive regulator of cys regulon require cystein (0.1 mM) L-broth (Luria, S. E. et. al. 1957. J. Bacteriol. 74: 461-476) L broth •unable to grow on O-acetyl-L-serine or sulfite Jagura-Burdzy, G. & D. Hulanicka
J. Bacteriol. 1981. 147: 744-751
cysC cystein adenylsaufate kinase require cystein (20 μg/ml), grow on 1 mM cysteine sulphinic acid minimal medium (J. Gen. Microbiol. 1961. 24: 335-353) supplemented with cystein (20μg/ml) and a carbon souce (glucose or lactose, 0.4 %) or Oxoid nutrient broth supplemented with cystein (20μg/ml) L broth •unable to grow on sulfate as the only sulfur source (J. Bio. Chem. 1988. 263: 2409-2416) Jones-Mortimer, M. C.
Heredity. 1973. 31: 213-221
cysD cystein sulfate acdnylyltransferase require cystein (20μg/ml), grow with 1 mM cysteine sulphinic acid minimal medium (J. Gen. Microbiol. 1961. 24: 335-353) supplemented with cystein (20μg/ml) and a carbon souce (glucose or lactose, 0.4 %) or Oxoid nutrient broth supplemented with cystein (20μg/ml) L broth •unable to grow on media containing sulfate as the only sulfur source (J. Bio. Chem. 1988. 263: 2409-2416) Jones-Mortimer, M. C.
Heredity. 1973. 31: 213-221
cysE cystein serine acetyltransferase require cystein or glutathione (both 0.4 mM) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Fimmel, A. L. & R. E. Loughlin
J. Gen. Microbiol. 1977. 103: 37-43
cysG cystein uroporphyrinoge III methyltransferase require cystein (15 μg/ml) Lennox broth L broth Macdonald, H. & J. Cole
Mol. Gen. Genet. 1985. 200: 328-334
cysH cystein adenylylsulfate reductase require cystein (20 μg/ml), grow on systein sulphonic acid (1 mM) minimal medium (J. Gen. Microbiol. 1961. 24: 335-353) supplemented with cystein (20μg/ml) and a carbon souce (glucose or lactose, 0.4 %) or Oxoid nutrient broth supplemented with cystein (20μg/ml) L broth Jones-Mortimer, M. C.
Heredity. 1973. 31: 213-221
cysI cystein cysQ sulfite reductase, alpha-subunit require cystein (20 μg/ml), but not grow on systein sulphonic acid (1 mM) minimal medium (J. Gen. Microbiol. 1961. 24: 335-353) supplemented with cystein (20μg/ml) and a carbon souce (glucose or lactose, 0.4 %) or Oxoid nutrient broth supplemented with cystein (20μg/ml) L broth Jones-Mortimer, M. C.
Heredity. 1973. 31: 213-221
dacA D-alanine carboxypeptidase pfv D-alanine carboxypeptidase IA; penicillin-binding protein sensitive to penisillin G (cross-streaking method) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth •sensitive to benzylpenicillin (10 μg/ml), ampicillin (2.5 μg/ml), and cephalexin (2.5 μg/ml) Nishimura, Y. et al.
J. Bacteriol. 1980. 143: 531-534
dacB D-alanine carboxypeptidase D-alanine carboxypeptidase IB; penicillin-binding protein unknown L medium L broth Takeda, Y. et al.
Plasmid. 1981. 6: 86-98
dacC D-alanine carboxypeptidase penicillin-binding protein 6 unknown L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Broome-Smith, J. K. & B. G. Spratt
J. Bacteriol. 1982. 152: 904-906
dadA D-amino acid dehydrogenase dadR D-amino acid dehydrogenase subunit form white colonies on tetrazolium agar plates (Bochner, and savageau 1977) supplemented with L- or D-alanine rich medium made from dry broth, from Biomed (Warszawa, Poland), of Difco (Detroit, USA) L broth •resistant to beta-chloro-D-alanine (The inhibitory effect was examined by applying 0.1 micro-mole aliquots of beta-chloro-D-alanine to filter paper discs on minimal agar plates overlayed with soft agar containing wild-type or mutant cells. The analog produced inhibition zones, with a diameter of about 3.5 cm, on wild-types strains only. ) •in trp- or other amino acid auxotroph: grow on D-tryptophan (20 μg/ml) or other D-amino acid (Proc. Natl. Acad. Sci. USA. 1971. 68: 2484-2487) Wild, J. & T. Klopotowski
Mol. Gen. Genet. 1981. 181: 373-378
dadB D-amino acid dehydrogenase alnA D-amino acid dehydrogenase subunit unable to grow on 0.5 % D-alanine or L-alanine as sole carbon source minimal medium containing per liter 6 g of Na2HPO4, 3 g of KH2PO4, 1 g of NH4Cl, 0.1 g of MgSO4, supplemented with 1 % glucose L broth Beelen, R. H. J. et al.
Mol. Gen. Genet. 1973. 121: 369-374
dam DNA adenine methylase DNA adenine methylase increase free phage in lysogenic cultures L-broth containing 10 g of tryptone, 5 g of yeast extract, and 5g of NaCl per liter L broth •, increase sensitivity to methyl methanesulfonate (0.05 M), increase sensitivity to UV •in recA-, recB-, or recC-: Vrm- inviability Marinus, M. G. & N. R. Morris
Mutat. Res. 1975. 28: 15-26
dapA diaminopimelate (lysine path) dihydropicolinate synthase require diamonopimelic acid (75 μg/ml in minimal medium or 50 μg/ml in rich medium) tryptone-yeast extract medium (L broth) supplemented with diaminopimelic acid (50 μg/ml) L broth supplemented with diaminopimelic acid (50 μg/ml) Bukhari, A. I. & A. L. Taylor
J. Bacteriol. 1971. 105: 844-854
dapC diaminopimelate (lysine path) require diaminopimelic acid (75 μg/ml in minimal medium or 50 μg/ml in rich medium) tryptone-yeast extract medium (L broth) supplemented with diaminopimelic acid (50 μg/ml) L broth supplemented with diaminopimelic acid (50 μg/ml) Bukhari, A. I. & A. L. Taylor
J. Bacteriol. 1971. 105: 844-854
dapD diaminopimelate (lysine path) require diaminopimelic acid (75 μg/ml in minimal medium or 50 μg/ml in rich medium) tryptone-yeast extract medium (L broth) supplemented with diaminopimelic acid (50 μg/ml) L broth supplemented with diaminopimelic acid (50 μg/ml) Bukhari, A. I. & A. L. Taylor
J. Bacteriol. 1971. 105: 844-854
dapE diaminopimelate (lysine path) dapB N-succinyl-diaminopimelate deacylase require diaminopimelic acid (75 μg/ml in minimal medium or 50 μg/ml in rich medium) tryptone-yeast extract medium (L broth) supplemented with diaminopimelic acid (50 μg/ml) L broth supplemented with diaminopimelic acid (50 μg/ml) Bukhari, A. I. & A. L. Taylor
J. Bacteriol. 1971. 105: 844-854
dcd dCTP deaminsase paxA dCTP deaminsase sensitive to 5-bromodeoxyuridine (100 μg/ml) at 42 °C L broth (Luria, S. E. et. al. 1957. J. Bacteriol. 74: 461-476) L broth Fuchs, J. A. & H. O. Karlstrom
J. Bacteriol. 1976. 128: 810-814
dcm DNA cytosine methylation mec DNA cytosine methylase resistant to 5-azacytidine (20 μg/ml) (see Notes) yeast extract tryptone medium L broth •This selection is available in E.coli B derivatives. K-12 derivatives are naturally resistant to 5-azacytidine. Friedman, S.
J. Bacteriol. 1982. 151: 262-268
ddlB D-alanine ligase dll D-alanine ligase sensitive to nonpermissive temperature (42 °C) in low osmotic condition yeast broth (Lugtenberg, E. J. J. & P. G. de Haan. 1971. J. Microbiol. Serol. 37: 537-552) L broth Lugtenberg, E. J. J. & v Schijndel-van Dam A.
J. Bacteriol. 1973. 113: 96-104
degP degradative protease htrA DegP protease, periplasmic endoprotease form reduced halo of alkaline phosphatase around colonies on the TYE plates containing 5-bromo-4-chloro-3-indolyl phosphate (150 μg/ml) TYE medium (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth Strauch, K. L. & J. Beckwith
Proc. Natl. Acad. Sci. USA. 1988. 85: 1576-1580
del deletion reduce the frequency of IS1-mediated deletion M9 minimal medium (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) supplemented with casamino acids and glycerol L broth Nevers, T. et al.
DNA insetion elements, plasmids and episomes. 1977. Cold Spring Harbor, N. Y. 125-128
deoA deoxyribose tpp-75 thymidine phosphorylase unable to grow on thymidine (5 mM) as the sole carbon source M9 supplemented with glucose (20 mM) L broth Ahmad, S. I. & R. H. Pritchard
Mol. Gen. Genet. 1969. 104: 351-359
deoB deoxyribose drm thyR tlr deoxyribouratase, phosphopentomutase unable to grow on thymidine, deoxyadnosine, or adenosine as the only source of carbon (all 0.1 %) glucose (glycerol) minimal medium L broth •in thy-: grow on low thymine (1-10 μg/ml) (J. Bacteriol. 1968. 96: 501-514) Hammer-Jespersen, K. et al.
Eur. J. Biochem. 1971. 19: 533-538
deoC deoxyribose dra thyR tlr deoxyribose-phosphate aldolase unable to grow on thymidine or deoxyadenosine as the only source of carbon (both 0.1 %) glucose (glycerol) minimal medium L broth •in thyA-: grow on low thymine (1-2 μg/ml)
•in deoD-: resistant to 2-deoxyadenoseine (0.1 %) (Mol. Gen. Genet. 1979. 176: 361-369) Hammer-Jespersen, K. et al.
Eur. J. Biochem. 1971. 19: 533-538
deoD deoxyribose pup deoxyribose-phosphate aldolase unable to grow on deoxyadnosine (0.1 %) as the only source of carbon glucose (glycerol) minimal medium L broth •in deoC- resistant to 2-deoxyadenoseine (0.1 %) (Mol. Gen. Genet. 1979. 176: 361-369) Hammer-Jespersen, K. et al.
Eur. J. Biochem. 1971. 19: 533-538
deoR deoxyribose nuc R nupG tse (regulatory gene for deo operator grow faster on inosine (0.1 %) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Much-Peterson, A. et al.
Eur. J. Biochem. 1972. 27: 208-215
dgk diglyceride kinase unable to grow well on nutrient media of low osmolarity (e.g. LB broth without NaCl) LB containing 10 g of NaCl, 10 g of Tryptone (Difco) and 5 g of yeast extract (Difco) L broth •in mdoB-: resistant to arbutin (15 mM or higher) (J. Bacteriol. 1984. 160: 976-981) Raetz, C. R. H. & K. F. Newman
J. Biol. Chem. 1978. 253: 3882-3887
dgoA D-galactonate 2-oxo-3-deoxygalactonate 6-phosphate aldose unable to grow on D-galactonate (10 mM) minimal medium 63 (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) supplemented with glycerol (20 mM) L broth Cooper, R. A.
Arch. Microbiol. 1978. 118: 199-206
dgt dGTPase optA deoxyguanosine 5'-triphosphate triphosphohydrolase sensitive but unable to support the grow of phage T7 defective in gene 1.2 L broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Saito, H. & C. C. Richardson
J. Virol. 1981. 37: 343-351
dksA dnaK suppressor msmA grow very poorly on M9 minimal medium supplemented with 20 amino acids and vitamins at all temperatures LB broth L broth •in dnaK-, grpE-, or dnaJ-: resistant to nonpermissive temperature (42° C) (suppress ts phenotypes of these mutations) Kang, P. J. & E. A. Craig
J. Bacteriol. 1990. 172: 2055-2064
dnaA DNA dnaH ? DNA biosynthesis; initiation, binding protein sensitive to nonpermissive temperature (42 °C) Oxoid No. 2 Nutrient Broth (Oxo Ltd) or M9 minimal medium supplememted with 0.2 % glucose L broth •It seems likely that dnaH mutation is in the dnaA gene. (J. Bacteriol. 1976. 128: 801-809) Wechsler, J. A & J. D. Gross
Mol. Gen. Genet. 1971. 113: 273-284
dnaB DNA groP grpA grpD DNA biosynthesis; chain elongation sensitive to nonpermissive temperature (42 °C) Oxoid No. 2 Nutrient Broth (Oxo Ltd) or M9 minimal medium supplememted with 0.2 % glucose L broth Wechsler, J. A & J. D. Gross
Mol. Gen. Genet. 1971. 113: 273-284
dnaC DNA dnaD DNA biosynthesis; initiation & chain elongation sensitive to nonpermissive temperature (42 °C) Oxoid No. 2 Nutrient Broth (Oxo Ltd) or M9 minimal medium supplememted with 0.2 % glucose L broth Wechsler, J. A & J. D. Gross
Mol. Gen. Genet. 1971. 113: 273-284
dnaE DNA polC sdgC DNA polymerase III, a-subunit sensitive to nonpermissive temperature (42 °C) Oxoid No. 2 Nutrient Broth (Oxo Ltd) or M9 minimal medium supplememted with 0.2 % glucose L broth Wechsler, J. A & J. D. Gross
Mol. Gen. Genet. 1971. 113: 273-284
dnaG DNA dnaP parB sdgA DNA polymerase III, a-subunit sensitive to nonpermissive temperature (42 °C) Oxoid No. 2 Nutrient Broth (Oxo Ltd) or M9 minimal medium supplememted with 0.2 % glucose L broth Wechsler, J. A & J. D. Gross
Mol. Gen. Genet. 1971. 113: 273-284
dnaJ DNA groP grpC shaperon with DnaK sensitive to nonpermissive temperature (43.5 °C) Tryptone/yeast broth containing 1 % Tryptone (Difco), 0.1 % yeast extract (Difco), 0.1 % glucose, and 0.8 % NaCl L broth •able to absorb lambda phages but unable to support their growths (J. Mol. Biol. 1977. 113: 1-25) Saito, H. & H. Uchida
Mol. Gen. Genet. 1978. 164: 1-8
dnaK DNA gro groP groPAB groPC groPF grpC grpF seq HSP-70-type molecular chaperone sensitive to nonpermissive temperature (43.5 °C) Tryptone/yeast broth containing 1 % Tryptone (Difco), 0.1 % yeast extract (Difco), 0.1 % glucose, and 0.8 % NaCl L broth •able to absorb lambda phages but unable to support their growths Saito, H. & H. Uchida
J. Mol. Biol. 1977. 113: 1-25
dnaQ DNA mutD DNA polymerase III Epsilon-subunit sensitive to nonpermissive temperature (44.5 °C) in salt-free L-broth L-broth containing 1% polypeptone (Daigo Eiyo Chem. Co., Japan), 0.5 % Difco yeast extract, 0.5 % NaCl, and 0.1 % glucose L broth •cause stimulation of rifampicin-, nalidixic acid-and streptomycin-resistant Degnen, G. E. & E. C. Cox
J. Bacteriol. 1974. 117: 477-487
dnaT DNA primasomal protein i sensitive to nonpermissive temperature (41 °C) M9 minimal medium L broth Lark, C. A. et al.
J. Bacteriol. 1978. 136: 1008-1017
dnaX DNA mutH dnaZ subunit of DNA plymerase III holoenzyme DNA elongation factor III; tau & gamma subunits sensitive to nonpermissive temperature (42 °C) yeast extract tryptone medium (Genetics. 1964. 49: 237-365) supplemented with 0.5 % NaCl L broth Henson, J. M. et al.
Genetics. 1979. 92: 1041-1059
drpA DNA, RNA biosynthesis sensitive to nonpermissive temperature (42 °C) tryptone-yeast extract broth L broth Lech, K. F. et al.
J. Bacteriol. 1985. 162: 117-123
dsdA D-serine deaminase D-serine deaminase sensitive to D-serine (500 ug/l) L-broth (Luria, S. E. et. al. 1960. Virology. 12: 348-390) L broth McFall, E.
Genetics. 1967. 55: 91-99
dut dUTPase dnaS sof deoxyuridinetriphosphatase sensitive to uracil (2 mM) nutrient broth (J. Bacteriol. 1976. 126: 1082-1088) L broth •sensitive to 5'-flu orodeoxyuridine (60 microM) Hochhauser, S. J. & B. Weiss
J. Bacteriol. 1978. 134: 157-166
dvl dye-visible light sensitive to sodium dodecyl sulfate (0.2 %) L medium containing 1 % Bacto-Tryptone, 0.5 % yeast extract, 0.5 % NaCl, and 0.1 % glucose L broth •highly sensitive to 2.5 microM toluidine blue plus light Wakayama, Y. et al.
J. Bacteriol. 1984. 159: 527-532
ecpD E. coli papD homolog possible pilin chaperone unknown (no mutant yet) Raina, S. et al.
J. Bacteriol. 1993. 175: 5009-5021
eda Enter-Douderoff aldose hga kdgA kga 2-keto-3-deoxygluconate 6-phosphate aldolase unable to grow on glucurnate, gluconate, or galacturonate (4 mg/ml) medium 63 (J. Biol. Chem. 1964.239: 2765-2771) supplemented wtih 1 % tryptone (Difco) and 0.4 % yeast extract (Difco) L broth •in uidA-: grow on glycerol + methylglucuronide (Mol. Gen. Genet. 1973. 120: 319-335) Fradkin, J. E. & D. G. Fraenkel
J. Bacteriol. 1971. 108: 1277-1283
edd Enter-Douderoff dehydratase phosphogluconate dehydratase grow slowly on gluconate (4 mg/ml) minimal medium 63 (J. Biol. Chem. 1964. 239: 2765-2771) supplemented with 1 % tryptone (Difco) and 0.4 % glucose L broth Zablotny, R. & D. G. Frankel
J. Bacteriol. 1967. 93: 1579-1581
endA endonuclease DNA specific endonuclease I: extensive DNA breakdown stained green by methyl green after toluene incubation ML medium containing (per 1000 ml) 10 g of tryptone, 5 g of yeast extract, 5 g of NaCl, 1g of glucose; pH 7.0 L broth Wright, M.
J. Bacteriol. 1971. 107: 87-94.
eno enolase enolase unable to grow on glucose (10 mM), glycerol (25 mM), or succinate (40 mM) as a single carbon source medium X containing 0.58 % Na2HPO4, 0.3 % KH2PO4, 0.1 % NH4Cl, 0.05 % NaCl, and 0.01 % MgSO4 supplemented with 1 μg/ml thiamine hydrochloride, 0.5 % tryptone, 40 mM sodium succinate and 4 mM glycerol; pH6.7 L broth without glucose Irani, M. & P. K. Maitra
Biochem. Biophys. Res. Commun. 1974. 56: 127-133
entA enterochelin 2,3-dihydro-2,3-dihidroxybenzoate dehydrogenase require 2,3-dihidroxybenzoate (10 microM) in iron-deficient media (about 2 microM of iron) containing succinate as a carbon source, or in iron-deficient media containing glucose as a carbon source and 2,2'-dipyridyl (0.1 mM) medium 56 (Biochim. Biophys. Acta. 1951. 7: 585-599) without FeSO4, supplemented with 2,3-dihydoroxybenzoate (10 microM), glucose (30 mM), enterochelin (10 microM), and ferrous sulfate (0.2 mM) L broth Young, I. G. et al.
J. Bacteriol. 1971. 106: 51-57
envN envelop affects envelop; defects osmotically remedied sensitive to nonpermissive temperature (42 °C) without 1 % NaCl or 12.5 % sucrose nutrient broth containing Oxoid Nutrient Broth no. 2 and 0.3 % Oxoid yeast extract L broth •sensitive to sodium deoxycholate (10 mg/ml), acridine orange (50 μg/ml), triphenyl tetrazolium chloride (500 μg/ml), actinomycin D (5 μg/ml), novobiocin (40μg/ml) on plates containing 1 % NaCl or 12.5 % sucrose Egan, A. F. & R. R. B. Russell
Genet. Res. 1973. 21: 139-152
esp efficiency site for phage site for efficient packaging of phage T1 unknown (no mutant yet) Drexler, H.
Mol. Gen. Genet. 1977. 152: 59-63
fabA fatty acid biosynthesis beta-hydroxydecanoylthioester dehydrase grow on oleate (0.005 %) but not on glucose, glycerol, acetate, or succinate as a carbon source (all 0.2 %) medium E (Fed. Proc. 1967. 26: 728-) supplemented with 0.2 % carbon source L broth •grow on glycerol with unsaturated fatty acid (e.g. 0.2 mM oleate) Silbert, D. F. & P. R. Vagelos
Proc. Natl. Acad. Sci. USA. 1967. 58: 1579-1586
fabD fatty acid biosynthesis malonyl-CoA-accyl carrier protein transacylase sensitive to nonpermissive temperature (42 °C) mineral medium containing Medium 63 salts, glycerol carbon source, and yeast extract (Proc. Natl. Acad. Sci. USA. 1972. 69: 3105-3109) L broth Harder, M. E. et al.
J. Biol. Chem. 1974. 249: 7468-7475
fabF fatty acid biosynthesis cvc fabJ vtr beta-ketoacyl-acyl carrier protein synthase II unknown rich broth containing 10 g of tryptone, 1 g of glucose, 1 g of yeast extract, and 5 g of NaCl (J. Bacteriol. 1972. 112: 206-211) L broth Garwin, J. L. et al.
J. Biol. Chem. 1980. 255: 3263-3265
fabZ fatty acid biosynthesis sefA (3R)-hydroxymyristoyl acyl carrier protein dehydrogenase unable to form colonies at 25 °C on ML broth plates ML broth containing per liter 5 g of yeast extract, 10 g of tryptone, 5 g of NaCl L broth Normark, S. et al.
J. Bacteriol. 1976. 128: 401-412
fadA fatty acid and degradation thiolase I unable to grow on oleate (5 mM) medium E or LB broth (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth Spratt, S. K. et al.
J. Bacteriol. 1984. 158: 535-542
fadB fatty acid and degradation oldB 3-hydroxyacyl-CoA dehydrogenase, 3-hydroxyacyl-CoA epimerase, dodecenoyl- CoA-Delta-isomerase, enoyle-CoA hydoratase, fatty acid oxidization-complex alpha-subunit unable to grow on oleate (5 mM) medium E or LB broth (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth Spratt, S. K. et al.
J. Bacteriol. 1984. 158: 535-542
fadD fatty acid and degradation oldD acyl-CoA synthetase unable to grow on oleate (0.1 %) M9 mineral salts medium supplemented with carbon source (0.5 % acetate, succinate, glucose, or tryptone) L broth Klein, K. et al.
Eur. J. Biochem. 1971. 19: 442-450
fadE fatty acid and degradation oldE unable to grow on oleate (0.1 %) M9 mineral salts medium supplemented with carbon source (0.5 % acetate, succinate, glucose, or tryptone) L broth Klein, K. et al.
Enu. J. Biochem. 1971. 19: 442-450
fadL fatty acid and degradation ttr fatty acid transport protein, outer membrane unable to grow on laurate (5 mM), myristate (4 mM), palmitate (3.5 mM), or oleate (3.0 mM) medium E (Cell. 1978 13: 387-398) supplemented with Brij 58 (4 mg/ml), acetate (0.4 %), and glucose (0.4 %) L broth Nunn, W. D. & R. W. Simons
Proc. Natl. Acad. Sci. USA. 1978. 75: 3377-3381
fadR fatty acid and degradation dec ole thdB regulatory gene for fad reguloon grow on decanoate (6 mM) as the sole carbon source medium E (Cell. 1978 13: 387-398) supplemented with Brij 58 (4 mg/ml), acetate (0.4 %), and glucose (0.4 %) L broth Nunn, W. D. & R. W. Simons
Proc. Natl. Acad. Sci. USA. 1978. 75: 3377-3381
feoA ferrous iron ferrous iron uptake system grow better with sodium citrate (10 mM) tryptone yeast extract medium containing per liter 8 g of tryptone, 5 g of yeast ectract, and 5 g of NaCl L broth Hantke, K.
FEMS Microbiol. Lett. 1987. 44: 53-57
feoB ferrous iron membrane protein of ferrous iron uptake system grow better with sodium citrate (10 mM) tryptone yeast extract medium containing per liter 8 g of tryptone, 5 g of yeast ectract, and 5 g of NaCl L broth Hantke, K.
FEMS Microbiol. Lett. 1987. 44: 53-57
fhuA ferric hydroxmate uptake T1 T5rec tonA OMP receptor for ferrichrome, colicin M, and phages T1, T5, and phi 80 unable to grow on ferrichrome, or ferricrocin (filter paper disk impregnated with 5 micro-l of ca. 1 mM solution) resistant to albomycin (0.05 ml of 0.3 mg/ml solution dropped at one site of the plate) L broth (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth •resistant to phage T5 Kadner, R. J. et al.
J. Bacteriol. 1980. 143: 256-264
fhuB ferric hydroxmate uptake hydroxamate-dependent iron uptake, cytoplasmic membrane component unable to grow on ferrichrome, or ferricrocin (filter paper disk impregnated with 5 micro-l of ca. 1 mM solution) resistant to albomycin (filter paper disk with 6 ug) L broth (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth Kadner, R. J. et al.
J. Bacteriol. 1980. 143: 256-264
fhuC ferric hydroxmate uptake hydroxamate-dependent iron uptake, cytoplasmic membrane component unable to grow on ferrichrome, resistant to albomycin (both filter paper disk with 6 ug) tryptone-yeast medium L broth Fecker, L. & V. Braun
J. Bacteriol. 1983. 156: 1301-1314
fhuD ferric hydroxmate uptake periplasmic binding protein for ferric hydrox transport unable to grow on ferrichrome, resistant to albomycin (both filter paper disk with 6 ug) tryptone-yeast medium L broth Fecker, L. & V. Braun
J. Bacteriol. 1983. 156: 1301-1314
fibB unknown unknown L broth L broth Heller, K. et al.
J. Bacteriol. 1985. 161: 896-903
fimB fimbriae pil recombinase? regulatory gene for fimA unable to agglutinate guinea pig erythrocytes L-broth without glucose L broth Klemm, P. et al.
Mol. Gen. Genet. 1985. 199: 410-414
fimC fimbriae pil pilB biosynthesis of fimbriae, periplasmic shaperone for type I fimbriae unable to agglutinate guinea pig erythrocytes L-broth without glucose L broth Klemm, P. et al.
Mol. Gen. Genet. 1985. 199: 410-414
fimD fimbriae pil pilC export and assembly of type I fimbriae outer membrane protein unable to agglutinate guinea pig erythrocytes L-broth without glucose L broth Klemm, P. et al.
Mol. Gen. Genet. 1985. 199: 410-414
fipB F1 phage morphogenesis of phage F1 resistant to phage lambda at 34 to 41.5 °C on glucose as the sole carbon source MTPA medium (Eur. J. Biochem. 1967. 1: 3-11) supplemented with 0.2 % glucose or maltose L broth Lopez, J. & R. E. Webster
J. Bacteriol. 1985. 163: 900-905
flgA flagella flaU (flagellar synthesis; flagellar regulon member) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & M. Simon
J. Bacteriol. 1973. 113: 105-113
flgE flagella flaK flagellar hook subunit protein (flagellar regulon member) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
flgG flagella flaL (flagellar synthesis; flagellar regulon member) basal rod protein resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
flgH flagella flaY flagellar synthesis; basal body L-ring protein resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
flgI flagella flaM (flagellar regulon member) basal body P-ring protein resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
flgK flagella flaS (flagellar synthesis; flagellar regulon member) hook-associated protein I resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
flgL flagella flaT (flagellar synthesis; flagellar regulon member) hook-associated protein resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
flhA flagella flaH (flagellar synthesis; flagellar regulon member) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
flhB flagella flaG (flagellar synthesis; flagellar regulon member) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
flhC flagella flaI (flagellar synthesis; regulatory gene) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
flhD flagella flbB transcriptional activator of Fla classII operon resistant to phage chi L-broth containing (Per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliA flagella flaD (regulation of late gene expression) delta transcription factor for class 3a and 3b operons resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliC flagella flaA hag (flagellar synthesis; flagellar regulon member) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliD flagella flbC hook-associated protein 2 resistant to phage chi L-broth containing (Per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliE flagella flaN (flagellar synthesis) basal body component resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliF flagella flaB flagellar basal body M-ring protein resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliG flagella flaBII (motor switching and energizing) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliH flagella flaBIII (flagellar biosynthesis) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliJ flagella flaO (flagellar biosynthesis) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliK flagella flaE hook filament junction (conrols hook length) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliM flagella cheC, flaA (flagellar synthesis; motor switching and energizing) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliN flagella motD flagellar switch protein resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliP flagella flaR (flagellar synthesis; flagellar regulon member) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliQ flagella flaQ (flagellar synthesis; flagellar regulon member) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fliR flagella flaP (flagellar synthesis; flagellar regulon member) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
fnr fumarate-nitrate-reductase frdB nirA nirR ossA regulatory gene for nitraite and nitrate reductases, hydrogenase, fumarate reductase unable to grow anaerobically glycerol or lactate minimal medium L-broth without glucose L broth Lambden, P. R. & J. R. Guest
J. Gen. Microbiol. 1976. 97: 145-160
folK folate dihidro-hydroxymethylprotein pyrophosphokinase unknown (no mutant yet) Talarico, T. L. et al.
J. Bacteriol. 1992. 174: 5971-5977
frr factor for ribosome recycling / release ribosome recycling factor unknown (essential) Janosi, L. et al.
Proc. Natl. Acad. Sci. USA. 1994. 91: 4249-4253
fruA fructose ptsF fructosephosphotransferase enzyme II unbale to grow on 0.5 mM fructose but grow on 20 mM fructose or on 10 mM glycerol nutrient broth or glycerol medium L broth Ferenci, T. & H. L. Kornberg, F. R. S.
Proc. R. Soc. Lond. B. Biol. Sci. 1974. 187: 105-119
fruK fructose fpk fruF fructose-1-phosphate kinase unable to grow on fructose or fructose 1-phosphate as sole carbon sources (5 or 10 mM) nutrient broth or glycerol medium L broth Ferenci, T. & H. L. Kornberg, F. R. S.
Proc. R. Soc. Lond. B. Biol. Sci. 1974. 187: 105-119
fruR fructose fruC shl cra repressor of fru and other catabolite-activated genes unable to grow on lactate (0.4 %) and pyruvate LB L broth Crasnier-Mednansky, M. et al.
Microbiology. 1997. 143: 785-792
ftsA filamentation, temperature sensitive divA (cell division, septation) sensitive to nonpermissive temperature (41 °C) Penassay broth (Difco antibiotic medium no. 3 (J. Bacteriol. 1977. 131: 293-305)) L broth •resistant to penicillin G (250 U/ml) Ricard, M. & Y. Hirota
J. Bacteriol. 1973. 116: 314-322
ftsD filamentation, temperature sensitive unknown sensitive to nonpermissive temperature (41 °C ) Penassay broth (Difco antibiotic medium no. 3 (J. Bacteriol. 1977. 131: 293-305)) L broth •resistant to penicillin G (250 U/ml) Ricard, M. & Y. Hirota
J. Bacteriol. 1973. 116: 314-322
ftsE filamentation, temperature sensitive cell division, ATP-binding protein sensitive to nonpermissive temperature (41 °C) Penassay broth (Difco antibiotic medium no. 3 (J. Bacteriol. 1977. 131: 293-305)) L broth Ricard, M. & Y. Hirota
J. Bacteriol. 1973. 116: 314-322
ftsI filamentation, temperature sensitive pbpB sep penicillin-binding protein 3 sensitive to nonpermissive temperature (42 °C) Penassay broth (Difco antibiotic medium no. 3) L broth •resistant to cephalexin (5 μg/ml) Spratt, B. G.
J. Bacteriol. 1977. 131: 293-305
ftsL filamentation temperature sensitivity cytoplasmic membrane protein, 13.6 kDa form filamentous cells (lethal) (NZY liquid broth differing from LB in that tryptone is replaced by NZ amine and the salt concentration is 8g of NaCl per liter) L broth Guzman, L.-M. et al.
J. Bacteriol. 1992. 174: 7716-7728
ftsQ filamentation temperature sensitivity septal protein sensitive to nonpermissive temperature (42 °C) Oxoid nutrient broth plus 50 μg/ml of thymine L broth Begg, K. J. et al.
J. Bacteriol. 1980. 144: 435-437
ftsW filamentation temperature sensitivity rodA FtsW sensitive to nonpermissive temperature (42 °C) modified Lennox broth containing 1 % Polypeptone (BBL Microbiology Systems), 0.5 % yeast extract, 0.1 % glucose, 20 mg/l of thymine and 0.5 % NaCl L broth Ishino, F. et al.
J. Bacteriol. 1989. 171: 5523-5530
ftsZ filamentation, temperature sensitive sfiB sulB inhibition of septation, GFP binding protein sensitive to nonpermissive temperature (43 °C) Oxoid nutrient broth L broth Lutkenhaus, J. F. et al.
J. Bacteriol. 1980. 142: 615-620
fucP fucose fucose permease unable to grow on fucose (2 mg/ml) as the sole source of carbon and energy LB broth L broth •form pale colonies on MacConkey fucose (10 mg/ml) plates •fuc-3072::Tn10 in CAG12079 (ME8840) and fuc-3154::Tn10kan in CAG12115 (ME8932) are fucP. Chakrabarti, T. et al.
J. Bacteriol. 1984. 157: 984-986
fur ferric uptake regulation ferric iron uptake, negative regulatory gene resistant to manganese (10 mM) tryptone yeast extract medium L broth Hantke, K
Mol. Gen. Genet. 1987. 210: 135-139
gadA glutamate deCase gadS glutamate decarboxylase grow on glutamate (0.5 %) minimal medium (Davis, B. D. & E. S. Mingioli. J. Bacteriol. 1950. 60:17-28) supplemented with succinate (0.5 %) L broth Halpern, Y. S. & H. E. Umbarger
J. Gen. Microbiol. 1961. 26: 175-183
gadR glutamate deCase regulatory gene for gad unknown minimal medium (Davis, B. D. & E. S. Mingioli. J. Bacteriol. 1950. 60:17-28) without citrate supplemented with glucose L broth Lupo, M. & Y. S. Halpern
J. Bacteriol. 1970. 103: 382-386
galE galactose UDP galactose 4-epimerase, hexose-1-phosphate udridylyltransferase sensitive to galactose antibiotic medium No. 3 (Difco) L broth •resistant to phage U3 (J. Virol. 1971. 8: 669-674) Fietta, A. et al.
Plasmid, 1981. 6: 78-85
galK galactose (galactose resistance) galactokinase form white colonies on MacConkey agar plate containing galactose Luria broth L broth •in galE-: resistant to galactose (Proc. Natl. Acad. Sci. USA. 1963. 50: 463-469)
•in galT-: resistant to galactose (Proc. Natl. Acad. Sci. USA. 1959. 45: 1786-1791)
•in galU-: resistant to galactose (Mol. Gen. Genet. 1968. 102-79-88) Grossiord, B. P et al.
J. Bacteriol. 2003. 185: 870-878
galM galactose alldose-1-epimerase grow slowly on phenyl-beta-D-galactoside with IPTG L-broth (Miller. 1972. J. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) L broth Bouffard, G. G. et al.
J. Mol. Biol. 1994. 244: 269-278
galP galactose D-galactose/H+symporter unknown double-strength Oxoid CM1 nutrient broth L broth •in ptsI- mgl-: unable to grow on glucose (10 mM) as a carbon source Riordan, C. & H. L. Kornberg, F. R. S.
Proc. R. Soc. Lond. B. Biol. Sci. 1977. 198: 401-410
galR galactose repressor of galETK operon form negative colonies on EMB galactose plates tryptone broth L broth Saedler, H. et al.
Mol. Gen. Genet. 1968. 102: 79-88
galT galactose galB galactose-1-phosphate uridylyltransferase unable to grow on and sensitive to galactose (1x10-2 - 1x10-4 M) ammonium sulphate mineral medium (Medium 56 (Biochim. Biophys. Acta. 1951. 7: 585-)or Medium A (1956. Carnegie Inst. Washingon Publication No. 612)) L broth Kalckar, H. M. et al.
Proc. Natl. Acad. Sci. USA. 1959. 45: 1776-1786
galU galactose glucose-1-P uridylyltransferase(UDP-glucose pyrophosphorylase) resistant to phage P1 tryptone broth L broth •resistant to ampicillin (J. Bacteriol. 1971. 108: 1210-1223)
•resistant to phage U3 (J. Virol. 1971. 8: 669-674) Franklin, N. C.
Virology. 1969. 38: 189-191
garA glucarate unable to grow on glucarate (30 mM) double strength nutrient broth L broth Robertson, A. M. et al.
J. Gen. Microbiol. 1980. 117: 377-382
garB glucarate D-glucarate utilization unable to grow on glucarate (30 mM) double strength nutrient broth L broth Robertson, A. M. et al.
J. Gen. Microbiol. 1980. 117: 377-382
gatA galactitol galactitol-specific enzyme IIA of phosphotransferase system (PTS) unable to grow on galactitol (0.2 %) minimal medium (J. Bacteriol. 1967. 93: 642-648) supplemented with casein acid hydrolysate (1 %) and glycerol (0.2 %) L broth Lengeler, J.
J. Bacteriol. 1975. 124: 26-38
gcd gluconid glucose dehydrogenase unknown L broth L broth Cleton-Jansen, A. M. et al.
J. Bacteriol. 1990. 172: 6308-6315
gdhA glutamate dehydrogenase glutamate dehydrogenase unknown Luria broth (J. Biol. Chem. 1971. 246: 6288-6296) L broth Pahel, G. et al.
J. Bacteriol. 1978. 133: 139-148
glcB glycolate malate synthase G unknown minimal medium (Proc. R. Soc. Lond., B, Biol. Sci. 1966. 165: 179-188) supplemented with 30 mM sodium glycolate, 20 mM sodium glyxylate, 20 mM sodium acetate, 10 mM glucose, and 0.5 % Casamino Acids (Oxoid) L broth •in aceB-: unable to grow on acetate as a sole carbon source (50 mM)(J. Bacteriol. 1982. 149: 173-180) Ornston, L. N. & M. K. Ornston
J. Bacteriol. 1969. 98: 1098-1108
glcC glycolate regulatory gene unable to grow on glycolate (30 mM) Luria broth L broth Pellicer, M.-T.et al.
J. Bacteriol. 1996. 178. 2151-2059
glcD glycolate regulatory protein transcriptional activator unable to grow on glycolate (30 mM) Luria broth L broth Pellicer, M.-T.et al.
J. Bacteriol. 1996. 178. 2151-2059
glcE glycolate glcF glycolate oxidase subunit, FeS protein unable to grow on glycolate (30 mM) Luria broth L broth Pellicer, M.-T.et al.
J. Bacteriol. 1996. 178. 2151-2059
glcG glycolate unknown unknown Luria broth L broth Pellicer, M.-T.et al.
J. Bacteriol. 1996. 178. 2151-2059
glgA glycogen glycogen synthase give colorless colonies by staining with iodine solution ( 0.2 % I2, 0.4 % KI) on nitrogen limiting glucose solid medium nutrient broth L broth Damotte, M & C. Lares
Mol. Gen. Genet. 1977. 150: 325-329
glgB glycogen 1,4-alpha-glucan branching enzyme give blue colonies by staining with iodine solution ( 0.2 % I2, 0.4 % KI) on nitrogen limiting glucose solid medium nutrient broth L broth Damotte, M & C. Lares
Mol. Gen. Genet. 1977. 150: 325-329
glgC glycogen glucose-1-phosphate adenylyltransferase give super-brown (dark brown) colonies by staining with iodine solution ( 0.2 % I2, 0.4 % KI) on nitrogen limiting glucose solid medium nutrient broth L broth Damotte, M & C. Lares
Mol. Gen. Genet. 1977. 150: 325-329
glmS glucosamine glucosamine-6-phosphate synthase require D-glucosamine(200 μg/ml) even in PPBE, L, or nutrient broth PPBE broth containing 1 % proteose peptone no. 3, 0.1 % beef extract, and 0.5 % NaCl, L broth containing 0.2 % glucose, 1 % tryptone, 0.5 % yeast extract, and 0.5 % NaCl, or nutrient broth containing 3 g of beef extract and 5 g of peptone per liter, all broth supplemented withD-glucosamine(200 μg/ml) L broth supplemented with D-glucosamine(200 μg/ml) Wu, H. C. & T. C. Wu
J. Bacteriol. 1971. 105: 455-466
glnA glutamine glutamine synthetase require glutamine (0.2 %) LB L broth supplemented with glutamine (0.2 %) •resistant to methylammonium (100 mM) in medium containing NH4Cl (0.5 mM), arginine, proline or glutamate (each 0.2 %) as nitrogen source (J. Gen. Microbiol. 1987. 133: 1631-1639) •in relA-: require glutamine (0.2 %) even in LB Powell, B. S. & D. L. Court
J. Bacteriol. 1998. 180: 1053-1062
glnD glutamine uridylyltransferase require glutamine (0.2 %) LB L broth glutamine (0.2 %) •in relA-: require glutamine (0.2 %) even in LB
•in ftsZ-: resistant to nonpermissive temperature(42 ° C) without glutamine supplementation in LB Powell, B. S. & D. L. Court
J. Bacteriol. 1998. 180: 1053-1062
glnP glutamine L-glutamate periplasmic binding protein unable to grow on glutamine (2 microM) as a sole source of carbon minimal medium containing minimal salts medium E (J. Biol. Chem. 1956. 218: 97-102) supplemented with 0.5 % carbon source L broth •resistant to gamma-glutamyl-hydrazide or L-methionine-DL-sullfoximine (both 30-40 μg/ml) Masters, P. S. & J.-S. Hong.
J. Bacteriol. 1981. 147: 805-819
glnV glutamine supE Su2 glutamine tRNA2 unknown nutrient broth L broth •in case of supE-: resistant to caffeine (0.2 to 0.8 %) Delvaux, A.-M. & R. Devoret
Mutat. Res. 1969. 7: 273-285.
glnX glutamine supE Su2 unknown nutrient broth L broth •in case of supE-: resistant to caffeine (0.2 to 0.8 %) Delvaux, A.-M. & R. Devoret
Mutat. Res. 1969. 7: 273-285.
glpA glycerol phosphate glycerol-3-phosphate dehydrogenase (anaerobic) large subunit unknown minral medium (J. Bacteriol. 1966. 93: 642-648) supplemented with 0.1 % casein hydrolysate L broth •in glpD-: unable to grow anaerobically on glycerol (0.02 M) or L-alpha-glycerophosphate (0.04 M of DL-alpha-glycerophosphate, D-isomoer not utilizable) as a sole carbon souce Kistler, W. S. & E. C. C. Lin
J. Bacteriol. 1971. 108: 1224-1234
glpD glycerol phosphate glyD glycerol-3-phosphate dehydrogenase unable to grow on glycerol (0.02 M) as a sole carbon source minral medium (J. Bacteriol. 1966. 93: 642-648) supplemented with 0.1 % casein hydrolysate L broth •resistant to streptozotosin (50 μg/ml) (Mol. Gen. Genet. 1980. 179: 49-54) •in glpA-: unable to grow anaerobically on glycerol (0.02 M) or L-alpha-glycerophosphate (0.04 M of DL-alpha-glycerophosphate, D-isomoer not utilizable) as a sole carbon souce (J. Bacteriol. 1971. 108: 1224-1234) Kistler, W. S. & E. C. C. Lin
J. Bacteriol. 1971. 108: 1224-1234
glpK glycerol phosphate glycerol kinase form yellow or yellow-white colonies on MacConkey-glycerol plates LB L broth Lupski, J. R. et tal.
J. Bacteriol. 1990. 172: 6129-6134
glpR glycerol phosphate repressor of glp operon unable to grow on either glycerol or L-alpha-glycerophosphate (0.2 %) (non-inducible mutant) basal medium (J. Bacteriol. 1967. 93: 642) supplemented with 0.2 % carbon source and 0.2 % casein hydrolysaste L broth •constitutive mutants: the tetrazolium spray method (Biochim Biophys Acta. 1962. 60: 422-424) depended upon the ability of colonies to reduce the dye with either glycerol or L-alpha-glycerophosphate as the indicator substrate without prior induction Cozzarelli, N. R. et al.
J. Mol. Biol. 1968. 31: 371-387
glpT glycerol phosphate sn-glycerol-3-phosphate permease resistant to phosphonomycin (0.3 mM) L broth or PPBE broth (J. Bacteriol. 1971. 105: 455-466) L broth •unable to utilize 0.4 % alpha-glycerol phosphate as a carbon source (J. Bacteriol. 1976. 128: 810-814) •in uidA-: resistant to fosfomycin (J. Gen. Microbiol. 1977. 103: 37-43) Venkateswaran, P. S. & H. C. Wu
J. Bacteriol. 1972. 110: 935-944
gltA glutamate glut icdB citrate synthase require glutamate (0.2 mg/ml) or alpha-ketoglutarate (0.2 %) minimal medium A (Davis, B. D. & E. S. Mingioli. J. Bacteriol. 1950. 60:17-28) minus citrate supplemented with 0.5 % glucose and 0.2 μg/ml of glutamate L broth Gilvarg, C. & B. D. Davis
J. Biol. Chem. 1956. 222: 307-309
gltB glutamate aspB ossB glutamate synthase, large subunit require aspartate or glutamate (0.2 %) Luria broth (J. Biol. Chem. 1971. 246: 6288-6296) L broth Pahel, G. et al.
J. Bacteriol. 1978. 133: 139-148
gltS glutamate glutamate permease grow on glutamate as a sole carbon source (0.5 %) minimal medium (Davis, B. D. & E. S. Mingioli. J. Bacteriol. 1950. 60:17-28) without citrate supplemented with a carbon source L broth Marcus, M. & Y. S. Halpern
J. Bacteriol. 1967. 93: 1409-1415
glyA glycine serine hydroxymethyltransferase require glycine (200 μg/ml) LB (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth Stauffer, G. V. et al.
Gene. 1981. 14: 63-72
gmhA lpcA tfrA phosphoheptose isomerase resistant to phages T4, T3, T7, and lambda L broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Curtiss III, R.
J. Bacteriol. 1965. 89: 28-40
gnd gluconate-P dehydrogenase gluconate-P dehydrogenase unknown minimal medium 63 (J. Biol. Chem. 1964. 239: 2765-2771) supplemented with 1 % tryptone (Difco) and 0.4 % glucose L broth Fraenkel, D. G.
J. Bacteriol. 1968. 95: 1267-1271
gor GSH oxidoreductase glutathione oxidoreductase unable to convert 5,5'-dithiobis-(2-nitrobenzoic acid) (Nbs2) to the yellow Nbs- anion oxoid nutrient broth (Proc. R. Soc. Lond. B. Biol. Sci. 1966. 399: 10-22) L broth Davis, N. K. et al.
J. Gen. Microbiol. 1982. 128: 1631-1634
gpt guanine-xanthine phosphotransferase glyD gpp gxu guanine-xanthine phosphoribosyltransferase resistant to guanine (50 μg/ml) + sulphanilamide (20 μg/ml) but sensitive to the addition of hypoxanthine (10 μg/ml) defined salts medium (minimal medium) (Experiments in Microbial Genetics. 1968. Oxford: Blackwell) L broth Bruce, I et al.
J. Gen. Microbiol. 1984. 130: 2489-2495
grpP unknown (no data)
guaA guanine GMP synthetase require guanine (20 μg/ml) L broth supplemented with guanine (20 μg/ml) L broth supplemented with guanine (20 μg/ml) •resistant to psicofuranine (0.5 or 1.0 mg/ml) (J. Biol. Chem. 1963. 238: 2797-2803 ) Lambden, P. R. & W. T. Drabble
J. Bacteriol. 1973. 115: 992-1002
guaB guanine guaR IMP dehydrogenase require guanine (20 μg/ml) L broth supplemented with guanine (20 μg/ml) L broth supplemented with guanine (20 μg/ml) •resistant to psicofuranine (0.5 or 1.0 mg/ml) (J. Biol. Chem. 1963. 238: 2797-2803 ) Lambden, P. R. & W. T. Drabble
J. Bacteriol. 1973. 115: 992-1002
guaC guanine GMP reductase resistant to guanosine (30 μg/ml) Luria Broth L broth Peterson, C.
J. Biol. Chem. 1999. 274: 5348-5356
gutM glucitol exhibit very weak fermentation on EMB plates containing 0.5 % glucitol when compared with wild type cells LB L broth Yamada, M. & M. H. Saier, Jr.
J. Mol. Biol. 1988. 203: 569-583
gutQ glucitol unknown (no mutant yet) Yamada, M. & M. H. Saier Jr.
J. Mol. Biol. 1988. 203: 569-583
gyrA gyrase hisW nalA parD nfxA norA cold shock regulon DNA gyrase subunitA resistant to nalidixic acid (40 μg/ml) BT broth containing 0.3 % Nutrient Broth (Difco), 0.5 % tryptone (Difco), and 0.5 % NaCl L broth Hane, M. W. & T. H. Wood
J. Bacteriol. 1969. 99: 238-241
gyrB gyrase Cou couA acrB himB nalC nalD pcbA hisU DNA gyrase, subunit B resistant to coumermycin (30 μg/ml) L broth L broth •sensitive to acriflavin (10 μg/ml) Hansen, F. G. & von Meyenburg, K.
Mol. Gen. Genet. 1979. 175: 135-144
hdhA hydroxysteroid deHase hsdh 7-alpha-hydroxysteroid dehydrogenase unknown (no mutant yet) Yoshimoto, T. et al.
J. Bacteriol. 1993. 175: 5730
hemA hemin hemin biosynthesis, glutamyl tRNA dehydrogenase require delta-aminolevulinic acid (50 μg/ml) nutrient medium L broth supplemented with delta-aminolevulinic acid (50 μg/ml) Sasarman, A. et al.
J. Bacteriol. 1968. 96: 570-572
hemF hemin popB sec coproporphyrinogen III oxidase, aerobic conditions sensitive to hydrogen peroxide (1.0 mM) Luria-Bertani (LB) broth containing 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl L broth Mukhopadhyay, S. and H. E. Schellhorn
J. Bacteriol. 1997. 179: 330-338
hemL hemin popC gsa glutamate-1-semialdehyde aminotransferase reauire delta-aminolevulinic acid (25 μg/ml) L broth supplemented with delta-aminolevulinic acid (25 μg/ml) L broth supplemented with delta-aminolevulinic acid (25 μg/ml) Ilag, L. L. et al.
J. Bacteriol. 1991. 173: 3408-3413
hflA high frequency lysogeny operon including hfg, hflX, -K, -C; part of complex mutL-hflC transcription unit give turbid plaques by phage lambda-cIII tryptone broth containing 1.0 % tryptone and 0.5 % NaCl or Luria broth L broth •unable to support the growth of phage lambda-c17 Belfort, M. & D. L. Wulff
J. Bacteriol. 1973. 115: 299-306
hisA histidine N-(5'-phospho-L-ribosylformimino)-5-amino-1-(5'-phosphoribosyl)-4-imidazolecarboxamide require histidine (100 μg/ml) Difco nutriend broth supplemented with 0.5 g of NaCl per liter L broth Goldschmidt, E. P. et al.
Genetics. 1970. 66: 219-229
hisB histidine imidazoleglycerolphosphate dehydratase, histidinol phosphatase require histidine (100 μg/ml) Difco nutriend broth supplemented with 0.5 g of NaCl per liter L broth Goldschmidt, E. P. et al.
Genetics. 1970. 66: 219-229
hisC histidine histidinol-phosphate aminotransferase require histidine (100 μg/ml) Difco nutriend broth supplemented with 0.5 g of NaCl per liter L broth Goldschmidt, E. P. et al.
Genetics. 1970. 66: 219-229
hisD histidine histidinol dehydrogenase require histidine (100 μg/ml) Difco nutriend broth supplemented with 0.5 g of NaCl per liter L broth Goldschmidt, E. P. et al.
Genetics. 1970. 66: 219-229
hisF histidine cyclase component of IGP synthase complex require histidine (100 μg/ml) Difco nutriend broth supplemented with 0.5 g of NaCl per liter L broth Goldschmidt, E. P. et al.
Genetics. 1970. 66: 219-229
hisG histidine ATP-phosphoribosyltransferase require histidine (100 μg/ml) Difco nutriend broth supplemented with 0.5 g of NaCl per liter L broth Goldschmidt, E. P. et al.
Genetics. 1970. 66: 219-229
hisH histidine amino transferase (AICAR-generating) component of HISHF IGP synthase reaction require histidine (100 μg/ml) Difco nutriend broth supplemented with 0.5 g of NaCl per liter L broth Goldschmidt, E. P. et al.
Genetics. 1970. 66: 219-229
hisI histidine hisE phosphoribosyl-ATP pyrophosphatase require histidine (100 μg/ml) Difco nutriend broth supplemented with 0.5 g of NaCl per liter L broth Goldschmidt, E. P. et al.
Genetics. 1970. 66: 219-229
hisS histidine histidyl-tRNA synthetase require histidine (40 μg/ml) at 37 °C rich medium containing 1 % tryptone, 0.5 % yeast extract, and 0.4 % glucose at 30 ° C L broth •grow more slowly at 42 ° C even in the presense of histidine in minimal medium
•grow more slowly above 37 ° C in rich medium Nass, G.
Mol. Gen. Genet. 1967. 100: 216-224
hlpA histone-like protein orf(skp) firA skp ompH histone-like protein HLP-1 unknown LB medium L broth •in rifampin-resistant strains: sensitive to rifampin (50 μg/ml) Dicker, I. B. & S. Seetharam
J. Bacteriol. 1991. 173: 334-344
hns histone (HNS)-like protein cur fimG topX pilG virR bglY drdX osmZ msyA irk drs drc H-NS (H1a) DNA-binding protein, histone-like form orange colonies on indicator plates containing salicin and bromothymol blue L-broth L broth •require isoleucine (50 μg/ml) (deletion mutant)
•form mucoidy colonies on minimal glucose medium at 29 ° C (Mol. Gen. Genet. 1980. 180: 425-428) Yamada, H. et al.
Mol. Gen. Genet. 1991. 230: 332-336
hofB homologous to fim (pil) genes of Pseudomonas hopB defective in stable maintenance of mini-F plasmids L medium containing 1 % Bacto Tryptone (Difco Laboratories), 0.5 % yeast extract, and 0.5 % NaCl L broth Niki, H. et al.
J. Bacteriol. 1988. 170: 5272-5278
hofC homologous to fim (pil) genes of Pseudomonas hopC defective in stable maintenance of mini-F plasmids L medium containing 1 % Bacto Tryptone (Difco Laboratories), 0.5 % yeast extract, and 0.5 % NaCl L broth Niki, H. et al.
J. Bacteriol. 1988. 170: 5272-5278
hpcR unknown LB (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth •K-12 is a strain naturally lacking the hpc genes. Marek, L. E. & J. M. Henson
J. Bacteriol. 1988. 170: 991-994
hppT unknown unknown L broth containing 1 % tryptone (Difco), 0.5 % yeast extract (Difco), 0.2 % glucose, and 0.5 % NaCl at pH7.3 L broth • in Tyr- strains: grow on p-hydroxyphenylpyruvate (20 μg/ml) instead of tyrosine Gelfand, D. H. & R. A. Steinberg
J. Bacteriol. 1977. 130: 429-440
hpt HGPRT hypoxanthine-guanine phosphoribosyltransferase unable to grow on hypoxanthine as purine source (15 μg/ml) basal salt medium supplemented with carbohydrates (0.2 %) and purines (15 μg/ml) L broth Jochimsen, B. et al.
Mol. Gen. Genet. 1975. 143: 85-91
hrpB helicase (RNA) -like protein unknown undescribed Moriya, H. et al.
Nucleic Acids Res. 1995. 23: 595-598
hsdM host specificity hs hsm hsp rm DNA methylase M resistant to serial infection by phages (lambda cI857 with the tenperature-sensitive repressor, and lambda b2cI defective in integration and the establishment of repression) tryptone broth containing 10 g of tryptone (Difco) and 5 g of NaCl per liter L broth Giudice, L. D.
J. Bacteriol. 1979. 137: 673-676
hsdR host specificity hs hsp hsr rm endonuclease R resistant to serial infection by phages (lambda cI857 with the tenperature-sensitive repressor, and lambda b2cI defective in integration and the establishment of repression) tryptone broth containing 10 g of tryptone (Difco) and 5 g of NaCl per liter L broth Giudice, L. D.
J. Bacteriol. 1979. 137: 673-676
hsdS host specificity hss specificty determent for hsdM and hsdR resistant to serial infection by phages (lambda cI857 with the tenperature-sensitive repressor, and lambda b2cI defective in integration and the establishment of repression) tryptone broth containing 10 g of tryptone (Difco) and 5 g of NaCl per liter L broth Giudice, L. D.
J. Bacteriol. 1979. 137: 673-676
htrE high temperature (requirement) (sequence homology with polin protein PapC) sensitive to nonpermissive temperature (43.5 °C) M9 minimal medium (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) supplemented with glucose (0.2 %), MgSO4 (1 mM), MgCl2 (3 mM), CaCl2 (0.1 mM), and FeCl3 (0.3 mM) L broth •reduce colony-forming ability on Luria-Bertani medium in the presence of a high salt concentration (e.g. 0.7 M NaCl) Raina, S. et al.
J. Bacteriol. 1993. 175: 5009-5021
hutC histidine utilization grow on histidine (0.2 %) as sole nitrogen source (see Notes) Luria broth (J. Biol. Chem. 1971. 246: 6288-6296) L broth •This gene is introduced From Klebsiella aerogenes and remains intact. Phenotype described above is therefore that of wild type.
•in gltB-: unable to grow on histidine (0.2 %) as sole nitrogen source Pahel, G. et al.
J. Bacteriol. 1978. 133: 139-148
iclR isocitrate lyase isocitrate lyase form blue colonies on X-gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 60 μg/ml) plates L-broth containing 1 % Tryptone (Difco), 1 % NaCl,, and 0.5 % yeast extract L broth Sunnarborg, A. et al.
J. Bacteriol. 1990. 172: 2642-2649
ihfA integration, host factor himA hid sequence-specific DNA-binding transctiptional activator, IHF, alpha subunit resistant to mutant phage lambda (lambda N-cam int-c) LB L broth Miller, H. I. & D. I. Friedman
DNA insetion elements, plasmids and episomes. 1977. Cold Spring Harbor, N. Y. 349-356
ihfB integration, host factor himD hip sequence-specific DNA-binding transcriptional activator, IHF, beta subunit resistant to phage (lambda cin1, lambda cI c17, phi80, or Mu) LB L broth Kikuchi, A. et al.
J. Mol. Biol. 1985. 183: 129-140
ileS isoleucine isoleucyl-tRNA synthetase require isoleucine (50 μg/ml) L-broth (Lennox, E. S. Virology. 1995. 1: 190-206) L broth Iaccarino, M. & P. Berg
J. Bacteriol. 1971. 105: 527-537
ileV isoleucine isoleucine tRNA1 unknown (no mutant yet)
ilvA isoleucin-valine ile threonine deaminase require isoleucine or alpha-ketobutyrate (both 0.15 mM) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth •resistant to glycylleucine (J. Bacteriol. 1972. 112: 142-147) Berg, C. M. et al.
Genetics. 1979. 93: 309-319
ilvC isoleucin-valine ilvA ketol-acid reductoisomerase require isoleucine and valine (both 0.15 mM) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Berg, C. M. et al.
Genetics. 1979. 93: 309-319
ilvD isoleucin-valine dehydroxyacid dehydrase require isoleucine and valine (both 0.15 mM) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Berg, C. M. et al.
Genetics. 1979. 93: 309-319
ilvE isoleucin-valine ilvC ilvJ branched-chain amino acid aminotransferase require isoleucine and valine (both 0.15 mM) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Berg, C. M. et al.
Genetics. 1979. 93: 309-319
ilvF isoleucin-valine resistant to valine (1.2 mM) mineral salts glucose medium L broth Alexander-Caudle, C. et al.
J. Bacteriol. 1990. 172: 3060-3065
ilvH isoleucin-valine brnP acetolactate synthase• resistant to valine (50 μg/ml) L broth (Virology. 1995. 1: 190-206) or nutrient broth containing per liter 5 go of NaCl and 8 g of nutrient broth (Difco) L broth •resistant to phenylalanylleucine (0.2 mM) and glycylleucine (0.5 mM)(Gallop, N., H. Tavori, and Z. Barak. J. Bacteriol. 149: 387-390 (1982)) •in ilvG- ilvI-: require isoleucin and valine De Felice, Maurilio et al.
J. Bacteriol. 1974. 120: 1058-1067
ilvI isoleucine-valine unknown minimal citrate (J. Biol. Chem. 1956. 218: 97-106) supplemented with glucose (0.4 %) L broth •in ilvG- ilvH-: require isoleucine and valine (50 μg/ml)
•in lvH-: sensitive to valine (1.5 mM) De Felice, M. et al.
J. Bacteriol. 1974. 120: 1068-1077
ilvU isoleucine-valine resistant to thiaisoleucine minimal medium (Davis, B. D. & E. S. Mingioli. J. Bacteriol. 1950. 60:17-28) with citrate omitted and 0.5 % glucose or L-broth L broth Fayerman, J. T. et al.
J. Biol. Chem. 1979. 254: 9429-9440
ilvY isoleucin-valine positive regulator for ilvC unknown L broth (J. Bacteriol. 1951. 62: 289-297) L broth Biel, A. J. & H. E. Umbarger
J. Bacteriol. 1981. 146: 718-724
IN(rrnD-rrnE) exhibit a slight reduction in growth L broth supplemented with glucose (0.3 %) L broth Hill, C. W. & B. W. Harnish
Proc. Natl. Acad. Sci. USA. 1981. 78: 7069-7072
katC catalase resistant to hydrogen peroxide (0.15 M for 20 minutes) LB broth (J. Bacteriol. 1989. 171: 1196-1198) L broth Volkert, M. R. et al.
J. Bacteriol. 1994. 176:1297-1302
katE catalase catalase hydroperoxidase III fail to evolve bubbles of oxygen with a drop of 30 % H2O2 on LB plate LB medium containing 10 g of tryptone (Difco), 5 g of yeast extract (Difco), and 10 g of NaCl per liter L broth Loewen, P. C.
J. Bacteriol. 1984. 157: 622-626
katG catalase catalase hydrogen peroxidase I fail to evolve bubbles of oxygen with a drop of 30 % H2O2 on LB plate LB medium containing 10 g of tryptone (Difco), 5 g of yeast extract (Difco), and 10 g of NaCl per liter L broth Loewen, P. C. et al.
J. Bacteriol. 1985. 162: 661-667
kdgK ketodeoxygluconate ketodeoxygluconokinase unable to grow on glucuronate or galacturonate (2 mg/ml) rich medium containing (per ml) 10 mg of Bactotryptone (Difco), 5 mg of yeast extract (Difco), and 10 mg of NaCl L broth Pouyssegur, J. & F. Stoeber
J. Bacteriol. 1974. 117: 641-651
kdpA potassium dependence kac K+-translocating ATPase Kdp subunit require more than 1 mM K for full growth ML broth containing per liter 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl •in kdpBC-trkAD- trkD-: require more than 100 mM K (Meth. Enzymol. 1988. 157: 655-667) Epstein, W. & M. Davies
J. Bacteriol. 1970. 101: 836-843
kdpB potassium dependence kac high-affinity potassium transport require more than 1 mM K for full growth ML broth containing per liter 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl •in kdpAC- trkAD- trkD-: require more than 100 mM K (Meth. Enzymol. 1988. 157: 655-667) Epstein, W. & M. Davies
J. Bacteriol. 1970. 101: 836-843
kdpC potassium dependence kac high-affinity potassium transport require more than 1 mM K for full growth ML broth containing per liter 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl •in kdpAB- trkAD- trkD-: require more than 100 mM K (Meth. Enzymol. 1988. 157: 655-667) Epstein, W. & M. Davies
J. Bacteriol. 1970. 101: 836-843
kdpD potassium dependence sensor kinase for K+ kdp system require more than 1 mM K for full growth ML broth containing per liter 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl Epstein, W. & M. Davies
J. Bacteriol. 1970. 101: 836-843
kdpE potassium dependence transcriptional effector of kdp operon unknown KML containing per liter 10 g of tryptone, 10 g of KCl, and 5 g of yeast extract •in trkA- trkD-: require approximately 20 mM K+ to achieve half the rate of growth seen above 100 mM K+ Polarek, J. W. et al.
J. Bacteriol. 1992. 174: 2145-2151
kdpF potassium dependence small peptide subunit of KdpABCF complex (stabilizes complex) unknown K10 medium containing 46 mM of Na2HPO4, 23 mM of NaH2PO4, 8 mM of (NH4)2SO4, 0.4 mM of MgSO4, 10 mM of KCl, and 6 microM of FeSO4 supplemented with 10 g/l of glucose Gassel, M. et al.
J. Biol. Chem. 1999. 274: 37901-37907
kefB K efflux trkB NEM-activatable K+/H+ antiporter unknown KML, a high-K version of ML medium, containing per liter 10 g of tryptone, 10 g of KCl, and 5 g of yeast extract L broth •in kdpABC-: require potassium (5 mM) Epstein, W. & B. S. Kim
J. Bacteriol. 1971. 108: 639-644
ksgB kasugamycin (high-level resistance to kasugamycin) resistant to kasugamycin (60-125 μg/ml) minimal medium A (Davis, B. D. & E. S. Mingioli. J. Bacteriol. 1950. 60:17-28) supplemented with carbohydrates (0.2 %) or L medium L broth Sparling, P. F. et al.
J. Bacteriol. 1973. 113: 704-710
lacA lactose galactoside acetyltransferase show inhibited growth on lactose or lactulose (alpha-D -galactosyl-beta-1,4-D-fructose) by adding isopropyl-beta-thiogalactose (IPTG, 5 mM) beta-galactoside (15 mM) mineral medium supplemented with bitamine B1 L broth Andrews, K. J. & E. C. C. Lin
J. Bacteriol. 1976. 128: 510-513
lacI lactose repressor protein o f lac operon form white or light blue colonies on mineral medium plates containing glucose (0.5 %) and 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (xgal, 40 μg/ml) Dyt-broth containing 1.6 % Bacto tryptone (Difco), 1.0 % yeast extract (Difco), 0.5 % NaCl L broth Pfahl, M.
Genetics. 1972. 72: 393-410
lacM unknown (no data)
lacY lactose galactoside permease (M protein) form white colonies on MacConkey lactose plates DYT broth containing 1.6 % Bacto tryptone (Difco), 1.0 % yeast extract (Difco), and 0.5 % NaCl L broth •form blue colonies on MacConkey-lactose plate containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (xgal, 0.04 g/li with 4 g/l of glucose as a carbon source) and ispropyl-thio-beta-D-galactoside (IPTG, 0.048 g/l) (WT: dark purple, lacZ-lacY-: white)
•resistant to o-nitrophenyl-beta-D-galactopyranoside (2 mM) (J. Bacteriol. 1973. 114: 882-884 ) Hobson, A. C. et al.
J. Bacteriol. 1977. 131. 830-833
lacZ lactose beta-D-Galactosidase form white colonies on lactose-tetrazolium indicator plates glycerol-M63 synthetic medium (J. Mol. Biol.1959. 11: 23-34) L broth •resistant to o-nitrophenyl-beta-Dgalactopyranoside (2 mM) (J. Bacteriol. 1973. 114: 882-884 Scaife, J. & J. R. Beckwith
Cold Spring Harb. Symp. Quant. Biol. 1966. 31: 403-408
lamB lambda phage lambda receptor protein resistant to phage lambda broth containing 1 % peptone (Difco), 0.5 % of meat extract (Liebig), and 0.5 % NaCl L broth Thirion, J. P., and M. Hofnung
Genetics. 1972. 71: 207-216
ldcC lysine decarboxylase lysine decarboxylase isozyme unknown LB medium L broth Kikuchi, Y. et al.
J. Bacteriol. 1997. 179: 4486-4492
leuA leucine alpha-isopropylmalate synthase require leucine (20 μg/ml) L broth (Lennox, E. S. Virology. 1955. 1: 190-206) L broth Somers, J. M. et al.
J. Bacteriol. 1973. 113: 1268-1272
leuB leucine beta-isopropylmalate dehydrogenase require leucine (20 μg/ml) L broth (Lennox, E. S. Virology. 1955. 1: 190-206) L broth Somers, J. M. et al.
J. Bacteriol. 1973. 113: 1268-1272
leuC leucine alpha-siopropylmate isomerase subunit require leucine (20 μg/ml) L broth (Lennox, E. S. Virology. 1955. 1: 190-206) L broth Somers, J. M. et al.
J. Bacteriol. 1973. 113: 1268-1272
leuD leucine alpha-siopropylmate isomerase subunit require leucine (20 μg/ml) L broth (Lennox, E. S. Virology. 1955. 1: 190-206) L broth Somers, J. M. et al.
J. Bacteriol. 1973. 113: 1268-1272
leuO leucine regulatory protein, LysR-like unknown TB medium (1984. Experiments with gene fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) L broth Ueguchi, C. et al.
J. Bacteriol. 1998. 180:190-193
lexA lambda exicision exrA recA spr umuA global regulator for SOS regulon sensitive to ionizing radiation, UV, and methylmethanesulfonate (0.02 M) LB broth or N broth containing 8 g of tryptone (Difco), 5 g of NaCl. 2 g of glucose, 2.5 g of Casamino acid, and 20 ug of thiamine per liter L broth Mount, D. W. et al.
J. Bacteriol. 1972. 112: 886-893
ligA ligase lig, dnaL, pdeC DNA ligase sensitive to UV at 40 °C glucose-salts medium L broth Pauling, C. & L. Hamm
Proc. Natl. Acad. Sci. USA. 1968. 60: 1495-1502
ligT ligase RNA ligase, 2'-5' unknown LB L broth Arn, E. A. & J. N. Abelson
J. Biol. Chem. 1996. 271: 31145-31153
lipA lipoate lipoate biosynthesis require lipoic acid (5 ng/ml) L-broth L broth Herbert, A. A. & J. R. Guest
J. Gen. Microbiol. 1968. 53: 363-381
lipB lipoate lipoyl-protein ligase require lipoic acid (5 ng/ml) rich broth (Virology. 1982. 120:287-306) or 2xYT medium (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth Boom, T. J. V. et al.
J. Bacteriol. 1991. 173: 6411-6420
lldD L-lactose dehydrogenase lct lctD L-lactose dehydrogenase, FMN dependent unable to grow on L-lactose (0.2 %) as a carbon source tryptone broth conatining 1% Difco Bacto-Tryptone and 0.5 % NaCl L broth Lin, R.-J. & C. W. Hill
J. Bacteriol. 1983. 156: 914-916
lon long form capR deg din muc ATP-dependent protease sensitive to UV, form mucoid colonies tryptone-yeast extract liquid medium (MTY) L broth Molnar, J. et al.
Genet. Res. 1977. 30: 13-20
lpcA lipopolysaccharide core gmhA yafI phosphaheptose isomerase supersensitive to novobiocin (40 μg/ml or less) nutrient broth containing per liter 10 g of polypeptone (Daigo Eiyo Kagaku Co.), 10 g of Ehrlich meat extract (Kyokuto Seiyaku Co.), and 2 g of NaCl L broth •resistant to phage T4 Tamaki, S. et al.
J. Bacteriol. 1971. 105: 968-975
lpd lipoamide dehydrogenase dhl lipoamide dehydrogenase require acetate plus succinate (both 2 mM) for best growth on minimal medium supplemented with glucose (0.2 %) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Guest, J. R. & I. T. Creaghan
J. Gen. Microbiol. 1973. 75: 197-210
lpp lipoprotein mlpA lpm lpo murein lipoprotein structural gene resistant to globomycin (25 μg/ml) L-broth (Proc. Natl. Acad. Sci. USA. 1969. 64: 957-961) L broth •sensitive to cationid dyes, detergents, and ethylenediaminetetraacetic acid (EDTA) (Mol. Gen. Genet. 1978. 167: 1-9) Zwiebel, L. J. et al.
J. Bacteriol. 1981: 145: 654-656
lpxA lipid A eXpression UDP-N-acetylglucosamine acyltransferase sensitive to nonpermissive tmperature (42 °C) LB or YT medium L broth Coleman, J. & C. R. H. Raetz
J. Bacteriol. 1988. 170: 1268-1274
lpxB lipid A expression pgsB lipid A disaccharide synthase sensitive to nonpermissive temperature (42 °C) LB broth (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) containing (per liter) 10 g of NaCl, 10 g of tryptone (Difco), and 5 g of yeast extract (Difco) L broth Nishijima, M. & Raetz, R. H.
J. Biol. Chem. 1979. 254: 7837-7844
lpxC lipid A eXpression envA asmB UDP-3-O(R-3-hydroxymyristoyl)GlcNac deacetylase sensitive to actinomycin D (10 μg/ml), rifampicin (1 μg/ml), and gentian violet (2 μg/ml) LB (J. Bacteriol. 1951. 62: 293-300) supplemented with medium E (J. Biol. Chem. 1956. 218: 97-106)and 0.2 % glucose L broth Normark, S.
Genet. Res. 1970. 16: 63-78
lpxD lipid A eXpression skp hlpA fir ssc firA UDP-O(R-3-hydroxymyristoyl)-glucosamine-N-acyltransferase sensitive to nonpermissive temperature (43 °C) LB broth L broth Kelly, T. M. et al.
J. Biol. Chem. 1993. 268: 19866-19874
lrb L-ribose (util.) L-ribose reductase, NADPH-linked activity grow on L-ribose (0.4 %) in the presense of a ribitol operon from E. coli C L L broth Trimbur, D. E. & R. P. Mortlock
J. Bacteriol. 1991. 173: 2459-2464
lysA lysine diaminopimelate decarboxylase require lysine (30 μg/ml) tryptone-yeast extract medium (L broth) L broth Bukhari, A. & A. L. Taylor
J. Bacteriol. 1971. 105: 844-854
lysA lysine diaminopimelate decarboxylase require lysine (30 μg/ml) tryptone-yeast extract medium (L broth) L broth Bukhari, A. & A. L. Taylor
J. Bacteriol. 1971. 105: 844-854
lysT lysine Su beta lysT alpha supG supL lysine tRNA (multiple loci lysQTVWYZ) unknown glucose minimal medium L broth •in case of supG or supL: suppress misssense mutation (ochre) Murgola, E. J. & F. T. Pagel
J. Bacteriol. 1983. 156: 917-919
mafB maintenance of F defective in replication of F-like plasmids (e.g. F, R386, and R222) at a high temperature (42 °C) Penassay broth (Difco), L-broth, or typtone broth supplemented with 0.2 % maltose L broth Wada, C. & T. Yura
J. Bacteriol. 1979. 140: 864-873
malA maltose malQPT cluster resistant to phage lambda broth containing 1% peptone (Difco), 0.5 % meat extract, and 0.5 % NaCl L broth including malQPT cluster Schwartz, M.
J. Bacteriol. 1966. 92: 1083-1089
malB maltose malG-M cluster resistant to phage lambda broth containing 1% peptone (Difco), 0.5 % meat extract, and 0.5 % NaCl L broth include malGFEK lamB malM cluster Schwartz, M.
J. Bacteriol. 1966. 92: 1083-1089
malE maltose maltose-binding protein,periplasmic form negative colonies on eosin methylene blue/maltose plate, unable to grow on maltose (10 mM) growth medium containing mineral salts (H1) medium), glycerol (50 mM), and maltose (5 mM) L broth Hazelbauer, G. L.
J. Bacteriol. 1975. 122: 206-214
malF maltose maltose transport complex, inner membraine-sapnning subunit form negative colonies on eosin methylene blue/maltose plate, unable to grow on maltose (10 mM) growth medium containing mineral salts (H1) medium), glycerol (50 mM), and maltose (5 mM) L broth Hazelbauer, G. L.
J. Bacteriol. 1975. 122: 206-214
malI maltose repressor of malXY operon unknown double yeast tryptone medium L broth Ehrmann, M. & W. Boos
J. Bacteriol. 1987. 169: 3539-3545
malP maltose blu maltodextrin phosphorylase form blue colonies on maltose Blu indicator plate (Blu phenotype) broth L broth Roehl, R. A. & R. T. Vinopal
J. Bacteriol. 1979. 139: 683-685
malQ maltose amylomaltase form white colonies on tetrazolium-maltose agar plates broth containing 1% peptone (Difco), 0.5 % meat extract, and 0.5 % NaCl L broth •form negative colonies on EMB plates Hatfield, D. et al.
J. Bacteriol. 1969. 98: 559-567
malT maltose positive regulator for mal regulon resistant to phage lambda broth containing 1 % peptone (Difco), 0.5 % of meat extract (Liebig), and 0.5 % NaCl L broth Hatfield, D. et al.
J. Bacteriol. 1969. 98: 559-567
malX maltose phosphotransferase enzyme II homolog unknown Luria broth supplemented with 0.2 % carbon source L broth Reidl, J. & W. Boos
J. Bacteriol. 1991. 173: 4862-4876
malY maltose C-S lyase, cystathionase unknown (no mutant yet) Luria broth supplemented with 0.2 % carbon source L broth Reidl, J. & W. Boos
J. Bacteriol. 1991. 173: 4862-4876
manA mannose pmi mannosephosphate isomerase resistant to streptozotosin (50 μg/ml) minimal medium (J. Bacteriol. 1967. 93: 642-648) L broth Lengeler, J.
Mol. Gen. Genet. 1980. 179: 49-54
manY mannose pel ptsM ptsP insensitive to lambda in a cross streak tryptone broth containing 1 % tryptone and 0.5 % NaCl L broth Scandella, D. & W. Arber
Virology. 1974. 58: 504-513
map methionine aminopeptidase methionine aminopeptidase unknown (essential) Ben-Bassat, A. et al.
J. Bacteriol. 1987. 169: 751-757
mdh malate dehydrogenase malate dehydrogenase unable to grow on malate (0.5 %) Antibiotic Medium No.3 (Difco) or minimal glucose (0.4 %) medium L broth Courtright, J. B. & U. Henning
J. Bacteriol. 1970. 102: 722-728
mdoB membrane-derived oligosaccharide phosphoglycerol transferase I activity unknown LB L broth •in dgk-: resistant to arbutin (15 mM or higher) Jackson, B. J. et al.
J. Bacteriol. 1984. 160: 976-981.
melA melibiose mel-7 alpha-galactosidase form negative colonies on EMB melibiose plates (no color change) LB broth L broth Schmitt, R.
J. Bacteriol. 1968. 96: 462-471
melB melibiose mel-4 thiomethylgalactoside permease II form negative colonies on EMB melibiose plates (no color change) LB broth L broth Schmitt, R.
J. Bacteriol. 1968. 96: 462-471
menC menaquinone o-saccinylbenzoate synthase II require 2-succinylbenzoate (0.1 mM) for anaerobic growth L broth L broth •unable to grow anaerobically on fumarate Guest, J. R.
J. Bacteriol. 1977. 130: 1038-1046
mesJ yaeN unknown unknown (no mutant yet) Kail, M. et al.
Yeast. 1996. 12: 799-807
metA methionine homoserine transsuccinylase require methionine (20 mg/l), cystathionine, or homocystein L broth L broth Ahmed, A.
Mol. Gen. Genet. 1973. 123: 299-324
metB methionine met1 met-1 cystathionine gamma-synthase require methionine (20 mg/l), cystathionine, or homocystein L broth L broth Ahmed, A.
Mol. Gen. Genet. 1973. 123: 299-324
metC methionine cystathionine beta-lyase require methionine (20 mg/l) or homocystein L broth L broth Ahmed, A.
Mol. Gen. Genet. 1973. 123: 299-324
metD methionine D-methionine transport unknown LB broth L broth •in methionine auxotrophs: unable to grow on D-methionine or N-acetyl-L-methionine (100 μg/ml) Kadner, R. J. & W. J. Watson
J. Bacteriol. 1974. 119: 401-409
metE methionine tetrahydropteroyltriglutamate methyltransferase require methionine (20 mg/l) or vitamin B12(0.1 mg/l) L broth L broth Ahmed, A.
Mol. Gen. Genet. 1973. 123: 299-324
metF methionine 5,10-methylenetetrahydrofolate reductase require methionine (20 mg/l) L broth L broth Ahmed, A.
Mol. Gen. Genet. 1973. 123: 299-324
metG methionine methionyl-tRNA synthase require methionine (20 mg/l) L broth L broth Ahmed, A.
Mol. Gen. Genet. 1973. 123: 299-324
metJ methionine repressor resistant to ethionine (2x10-3M) LB L broth Su, C.-H. & R. C. Greene
Proc. Natl. Acad. Sci. USA. 1971. 68: 367-371
metK methionine methionine adenosyltransferase resistant to ethionine (2 mM) LB broth L broth •50 μg/ml of leucine should be supplemented for the cells not to be filamentous (J. Bacteriol. 1998. 180: 3614-3619) Greene, R. C. et al.
J. Bacteriol. 1973. 115: 57-67
mglA methyl-galactoside mglP methyl-galactoside permease, cytoplasmic membrane protein(methyl-galactoside transport and galactose taxis) unable to form a ring on a galactose swarm plate tryptone broth containing 1 % tryptone and 0.5 % NaCl L broth Ordal, G. W. & J. Adler
J. Bacteriol. 1974. 117: 509-516
mglB methyl-galactoside methyl-galactoside permease, galactose-binding protein(receptor for galactose taxis) unable to form a ring on a galactose swarm plate tryptone broth containing 1 % tryptone and 0.5 % NaCl L broth Ordal, G. W. & J. Adler
J. Bacteriol. 1974. 117: 509-516
mglC methyl-galactoside mglP methyl-galactoside permease(methyl-galactoside transport and galactose taxis) unable to form a ring on a galactose swarm plate tryptone broth containing 1 % tryptone and 0.5 % NaCl L broth Ordal, G. W. & J. Adler
J. Bacteriol. 1974. 117: 509-516
minA minicells positioning division septum form minicells (?) nutrient broth L broth Adler, H. I. et al.
Proc. Natl. Acad. Sci. USA. 1967. 57: 321-326
minB minicells positioning division septum form minicells nutrient broth L broth Adler, H. I. et al.
Proc. Natl. Acad. Sci. USA. 1967. 57: 321-326
mltB membrane-bound lytic transglycosylase slt murein hydrase lopoprotein unknown (no mutant yet) Ehlert, K. et al.
Mol. Microbiol. 1995. 16: 761-768
mltD membrane-bound lytic transglycosylase dniR unknown LB L broth Kajie, S. et al.
FEMS Microbiol. Lett. 1991. 83: 205-212
modA molybdenum periplasmic molybdate-binding protein resistant to potassium chlorate (0.2 %) (The procedure is described in Notes.) L broth supplemented with glucose (0.3 %) L broth •selection procedure: The cells are diluted into 10 ml of molten L agar containing 0.2 % potassium chlorate. The agar is poured into a petri dixh, allowed to harden, and then overlaid with 15 ml of L agar plus 0.2 % potassium chlorate. Maupin-Furlow, J. A. et al.
J. Bacteriol. 1995. 177: 4851-4856
modB molybdenum chlJ membrane-spanning component of molybdate transport (ABC) system resistant to potassium chlorate (0.2 %) (The procedure is described in Notes.) L broth supplemented with glucose (0.3 %) L broth •selection procedure: The cells are diluted into 10 ml of molten L agar containing 0.2 % potassium chlorate. The agar is poured into a petri dixh, allowed to harden, and then overlaid with 15 ml of L agar plus 0.2 % potassium chlorate. Maupin-Furlow, J. A. et al.
J. Bacteriol. 1995. 177: 4851-4856
modC molybdenum narD chlD molybdate transport ATP-binding protein, ATPase resistant to potassium chlorate (0.2 %) (The procedure is described in Notes.) L L broth •unable to form nitrate when stabed on L-agar in a test tube (If the strain produces a gas, the top layer of agar is forced up the tube as a result of gas evolution.) •selection procedure: The cells are diluted into 10 ml of molten L agar containing 0.2 % potassium chlorate. The agar is poured into a petri dixh, allowed to harden, and then overlaid with 15 ml of L agar plus 0.2 % potassium chlorate. Glaser, J. H. & J. A. DeMoss
J. Bacteriol. 1971. 108: 854-860
modE molybdenum modR repressor of mod (molybdate transport operon) unknown Luria broth supplemented with 0.3 % glucose L broth Grunden, A. M. et al.
J. Bacteriol. 1996. 178: 735-744
modF molybdenum phrA unknown Luria broth supplemented with 0.3 % glucose L broth Grunden, A. M. et al.
J. Bacteriol. 1996. 178: 735-744
motA motility flaJ (flagellar-regulon member; flagellar rotation) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
motB motility flaJ (flagellar-regulon member; flagellar rotation) resistant to phage chi L-broth containing (per liter) 10 g (Difco) of tryptone, 10 g of NaCl, 5 g of yeast extract (Difco), 2 g of glucose, 0.1 g of thymine L broth Silverman, M. & Simon, M.
J. Bacteriol. 1973. 113: 105-113
mraY murein cluster a UDP-N-acetylmuramoyl-pentapeptide undecaprenyl-PO4 phospho-N-acetylmuramoyl-pentapeptide transferase unknown (no mutant yet) Ikeda, M. et al.
J. Bacteriol. 1991. 173: 1021-1026
mrcA murein cluster c ponA penicillin-binding protein 1A unknown L broth containing 1 % NaCl L broth Suzuki, H. et al.
Proc. Natl. Acad. Sci. USA. 1978. 75: 664-668
mrcB murein cluster c ponB penicillin-binding protein 1Bs hypersensitive to cephalosporins such as cephaloridine (0.05 μg/ml) L broth containing 1 % NaCl L broth Suzuki, H. et al.
Proc. Natl. Acad. Sci. USA. 1978. 75: 664-668
mrdB murein cluster d rodA affects cell shape resistant to mitomycin C (4 μg/ml) nutrient broth containing per liter 5 g of polypeptone (Daigo Eiyo Kagaku Co.) , 10 g of Ehrlich meat extract (Kyokuto Seiyaku Co.), and 2 g of NaCl, or L-broth containing per liter 10 g of polypeptone, 5 g of yeast extract (Kyokuto Seiyaku Co.), and 5 g of NaCl supplemented with1 g of glucose L broth Matsuzawa, H. et al.
J. Bacteriol. 1973. 115: 436-442
mreB murein cluster e envB mon rodY resistant to mecillinam (10 μg/ml) medium (J. Bacteriol. 1974. 117: 578-587) containing 17.5 g of Difco antibiotic medium 3 and 5 g of yeast extract per liter L broth Iwaya, M. et al.
J. Bacteriol. 1978. 133: 196-202
mtlA mannitol mtlC mannitol-specific enzyme II of PTS form white colonies on MacConkey mannitol plates basal minimal medium (J. Bacteriol. 1967. 93: 642-648) supplemented with 1 % Casein acid hydrolysate and 0.2 % carbon source (except for mannitol) L broth Solomon, E. & E. C. C. Lin
J. Bacteriol. 1972. 111: 566-574
mtlD mannitol mannitol-1-phosphate dehydrogenase form white colonies on MacConkey mannitol plates basal minimal medium (J. Bacteriol. 1967. 93: 642-648) supplemented with 1 % Casein acid hydrolysate and 0.2 % carbon source (except for mannitol) L broth Solomon, E. & E. C. C. Lin
J. Bacteriol. 1972. 111: 566-574
mtlR mannitol mannitol receptor unknown Luria-Bertani (LB) broth L broth Figge, R. M. et al.
J. Bacteriol. 1994. 176: 840-847
mtr methyltryptophan high-affinity Trp permease resistant to 5-methyltryptophan (5 μg/ml) minimal glucose medium containing 0.2 % Casamino Acids L broth Hiraga, S. et al.
J. Bacteriol. 1968. 96: 1880-1881
murC murein L-alanine adding enzyme sensitive to nonpermissive temperature (42 °C) on yeast-agar yeast broth (J. Microbiol. Serol. 1971. 37: 537-552) L broth Lugtenberg, E. J. J. & v Schijndel-van Dam A.
J. Bacteriol. 1972. 110: 35-40
murD murein UDP-N-acetylmuramoyl-L-alanine: D-glutamate ligase unknown (no mutant yet) Menglin-Lecreulx, D. et al.
J. Bacteriol. 1989. 171: 6126-6134
murE murein meso-diaminopimelate adding enzyme sensitive to nonpermissive temperature (42 °C) on yeast-agar yeast broth (J. Microbiol. Serol. 1971. 37: 537-552) L broth Lugtenberg, E. J. J. & v Schijndel-van Dam A.
J. Bacteriol. 1972. 110: 41-46
murF murein mra D-alanine adding enzyme sensitive to nonpermissive temperature (42 °C) on yeast-agar yeast broth (J. Microbiol. Serol. 1971. 37: 537-552) L broth Lugtenberg, E. J. J. & v Schijndel-van Dam A.
J. Bacteriol. 1972. 110: 35-40
murG murein UDP-NAc-glucosamine: NAcmuramyl-(pentapeptide) pyrophos'ryl-undecaprenol NAc-glucosamine transferase sensitive to nonpermissive temperature (42 °C) Oxoid nutrient broth L broth Salmond, G. P. C. et al.
J. Bacteriol. 1980. 144: 438-440
mutS mutator ant? fdv (methyl directed mismatch repair) resistant to azaserine (200 μg/ml) nutrient broth containing 0.8 % Difco Nutrient Broth and 0.5 % NaCl L broth Siegel, E. C. & V. Bryson
J. Bacteriol. 1967. 94: 38-47
mutT mutator nudA nucleoside diphosphate-X hydrolase MutT (NudA) unknown L-broth L broth •cause A:T ---> C:G transversions and then increase the mutation rates in the absense of mutagens Cox, E. C.
Genetics Supplement. 1973. 73: 67-80
nadA nicotinamide adenine dinucleotide quinolinate synthetase, A protein require quinolinate or nicotinic acid (1 microM) miniimal Yates Pardee (YP) medium containing (per liter) 2 g of K2HPO4, 7 g of KH2PO4, 2.5 g of glucose, 0.5 g of sodium citrate•H2O, 1 g of (NH4)2SO4, 0.1 g of MgSO4•H2O, supplemented with nicotinic acid (1 microM) L broth Flachmann, R.et al.
Eur. J. Biochem. 1988. 175: 221-228
nadB nicotinamide adenine dinucleotide quinolinate synthetase, B protein require nicotinic acid (1 microM) miniimal Yates Pardee (YP) medium containing (per liter) 2 g of K2HPO4, 7 g of KH2PO4, 2.5 g of glucose, 0.5 g of sodium citrate•H2O, 1 g of (NH4)2SO4, 0.1 g of MgSO 4•H2O, supplemented with nicotinic acid (1 microM) L broth •Nicotinic acid requirement can be satisfied by nicotinamide (0.1 mM) (J. Bacteriol. 1976. 126: 1082-1088) •in pnc-: require nicotinic acid but unable to grow on nicotinamide (0.1 mM) (J. Bacteriol. 1976. 126: 1082-1088) Flachmann, R. et al.
Eur. J. Biochem. 1988. 175: 221-228
nadC nicotinamide adenine dinucleotide quinolinate phosphoribosyl transferase require nicotinic acid (0.005 mg/ml) Davis and Mingioli (J. Bacteriol. 1950. 60:17-28) minimal medium supplemented with nicotinic acid (0.005 mg/ml) L broth Trits, G. J. et al.
J. Bacteriol. 1970. 104: 45-49
nagA N-acetylglucosamine N-acetylglucosamine-6-phosphate deacetylase resistant to N-iodoacetyl-D-glucosamine (0.05 mM) 1.3 % (w/v) nutrient broth L broth White, R. J. & P. W. Kent
Biochem. J. 1970. 118: 81-87
nagB N-acetylglucosamine glmD N-acetylglucosamine-6-phosphate deaminase resistant to N-iodoacetyl-D-glucosamine (0.05 mM) 1.3 % (w/v) nutrient broth L broth White, R. J. & P. W. Kent
Biochem. J. 1970. 118: 81-87
nalB nalidixic acid (sensitivity to nalidixic acid) resistant to nalidixic acid (4 μg/ml) BT broth containing 0.3 % Nutrient Broth (Difco), 0.5 % tryptone (Difco), and 0.5 % NaCl L broth Hane, M. W. & T. H. Wood
J. Bacteriol. 1969. 99: 238-241
nlpE new lipoprotein cutF outer membrane lopoprotein NlpE / CutF sensitive to copper (4 mM CuSO4) L-broth (LB) L broth •sensitive to nonpermissive temperature (42 ° C) Gupta, S. et al.
J. Bacteriol. 1995. 177: 4207-4215
non nonmucoid (affects capsule formation) unknown M-9 minimal medium L broth •in capR-(mucoid): form nonmucoid cells Lieberman, M. M. & A. Markovitz
J. Bacteriol. 1970. 101: 965-972
nrdA nucleotide reductase dnaF ribonucleoside diphosphate reductase subunit B1 sensitive to nonpermissive temperature (42 °C) Oxoid No. 2 Nutrient Broth (Oxo Ltd) or M9 minimal medium supplememted with 0.2 % glucose L broth Wechsler, J. A & J. D. Gross
Mol. Gen. Genet. 1971. 113: 273-284
nrdB nucleotide reductase ftsB ribonucleoside diphosphate reductase subunit B2 sensitive to hydroxyurea (0.5 mg/ml) on L agar minus NaCl L broth L broth •sensitive to nonpermissive temperature (42 ° C) on L agar minus NaCl Kren, B. & J. A. Fuchs
J. Bacteriol. 1987. 169: 14-18
nupC nucleoside permease cru nucleoside permease (transport of nucleoside, except guanosine) unknown LB broth L broth •in nupG-: resistant to 5'-azacytidine (10 μg/ml, 10 micro-l/disc)
•in nupG-: resistant to resistant to fluorouracil (2.5 μg/ml), fluorouridine (10 μg/ml), and fluorocytidine (10 μg/ml) (J. Cell. Physiol. 1976. 89: 551-560) Craig, J. E. et al.
Mol. Microbiol. 1994. 11: 1159-1168
nupG nucleoside permease gru nucleoside permease sensitive to showdomycin (0.16 mM) phosphate-buffered minimal medium containing 15 mM of (NH4)2SO4, 42 mM of Na2HPO4, 50 mM of NaCl, 22 mM of KH2PO4, 2 mM of MgCl2, 0.1 mM of CaCl2, and 0.003 mM of FeCl3 supplemented with glycerol (0.2 %) L broth •unable to grow on guanine as sole carbon source •in nupC-: resistant to resistant to fluorouracil (2.5 μg/ml), fluorouridine (10 μg/ml), and fluorocytidine (10 μg/ml) Munch-Petersen, A. & B. Mygind
J. Cell. Physiol. 1976. 89: 551-560
nusA N(lambda protein) utilization substance antitermination L factor resistant to phage lambda delivatives (lambda cI857, lambda imm434 lambda c17cI, lambda imm21) at 42 °C tryptone broth L broth Friedman, D. I.
The Bacteriophage lambda 733-738
ompA outer membrane protein con tolG tut outer membrane protein 3a (II*;G;d) resistant to phage TuII* antibiotic medium no. 3 (Difco) L broth •tolerant to bacteriocin JF246(J. Bacteriol. 1974. 117: 1354-1355) Datta, D. B. et al.
J. Bacteriol. 1976. 128: 834-841
ompC outer membrane protein meoA par outer membrane protein 1b resistant to phage PA-2 Luria broth (LB) L broth •resistant to phage Mel (Mol. Gen. Genet. 1977. 150: 103-105) Bassford, P. J. et al.
J. Bacteriol. 1977. 131: 608-622
ompF outer membrane protein cmlB coa cry tolF outer membrane protein 1a (Ia, b, F) resistant to chloramphenicol (5 μg/ml) polypeeptone-glucose broth or L broth L broth Sato, T. & T. Yura
J. Bacteriol. 1979. 139: 468-477
ompR outer membrane protein cry envZ ompB activator protein for osmoregulation of OmpC and OmpF resistant to hy-2 phage LB L broth Wanner, B. L. et al.
J. Bacteriol. 1979. 140: 229-239
ompT outer membrane protein outer membrane protein 2b, protease VII unknown LB medium supplemented with 0.2 % glucose L broth Baneyx, F. & G. Georgiou
J. Bacteriol. 1990. 172: 491-494
oppE oligopeptide permease oligopeptide transport resistant to oligopeptides such as Val3 (100 ug on a filter paper disk), Leu3 (400 ug), Leu-Gly-Gly (400 ug), Val2(150 ug), and Ala-Ala-P (150 ug) complete minimal medium containing minimal salts described by Vogel and Bonner (J. Biol. Chem. 1956. 218:97-106) supplemented with glucose (0.2 %) L broth Andrews, J. C. & S. A. Short
J. Bacteriol. 1985. 161: 484-492
pabA para-aminobenzoate p-aminobenzoate biosynthesis require p-aminobenzoic acid (10-6 M) broth (Luria and Burrous. J. Bacteriol. 74: 461-476) supplemented with 0.1 % glucose L broth Huang, M. & J. Pittard
J. Bacteriol. 1967. 93: 1938-1942
pabB para-aminobenzoate p-aminobenzoate biosynthesis require p-aminobenzoic acid (10-6 M) broth (Luria and Burrous. J. Bacteriol. 74: 461-476) supplemented with 0.1 % glucose L broth Huang, M. & J. Pittard
J. Bacteriol. 1967. 93: 1938-1942
panB pantothenate ketopantoate hydroxymethyltransferase require pantothenic acid, pantoic acid (or its lactone), or ketopantoic acid (or its lactone) (0.01 %) R broth containing (per lliter) 10 g of tryptone, 1 g of glucose, 1 g of yeast extract, 5 g of NaCl (J. Bacteriol. 1972. 112: 206-211) L broth Cronan, Jr, J. E.
J. Bacteriol. 1980. 141: 1291-1297
panC pantothenate pantothenate synthetase require pantothenic acid (0.01 %) R broth containing (per lliter) 10 g of tryptone, 1 g of glucose, 1 g of yeast extract, 5 g of NaCl (J. Bacteriol. 1972. 112: 206-211) L broth Cronan, Jr, J. E.
J. Bacteriol. 1980. 141: 1291-1297
panD pantothenate aspartate 1-decarboxylase require beta-alanine or pantothenate (0.01 %) R broth containing (per lliter) 10 g of tryptone, 1 g of glucose, 1 g of yeast extract, 5 g of NaCl (J. Bacteriol. 1972. 112: 206-211) L broth Cronan, Jr, J. E.
J. Bacteriol. 1980. 141: 1291-1297
panF pantothenate pantothenate permease (symoporter) unknown rich broth containing per liter10 g of tryptone 5 g of NaCl 1 g of yeast extract supplemented with 20 microM beta-alanine L broth •in pantothenate auxotrophs: unable to grow on pantothenate(1 microM) Vallari, D. S. & C. O. Rock
J. Bacteriol. 1985. 164: 136-142
pbpS unknown (no data)
pcnB plasmid copy number polyA polymerase I reduce plasmid copy number of pBR322 and its derivatives LB medium L broth Lopilato, J. et al.
Mol. Gen. Genet. 1986. 205: 285-290
pdhR pyruvate dehydrogenase genA pyruvate dehydrogenase repressor unknown L-broth L broth Haydon, D. J. et al.
FEBS Lett. 1993. 336: 43-47
pdxA pyridoxine pyridoxine biosynthesis require pyridoxine or pyridoxine phosphate (6.0 microM) L-broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth •unable to grow on 2'-hydroxypyridoxine (0.4 mM) Hockney, R. C. & T. A. Scott
J. Gen. Microbiol. 1979. 110: 275-238
pepD peptides peptidase D, a dipeptidase unknown nutrient broth (Difco) or LB medium L broth Miller, C. G. & G. Schwartz
J. Bacteriol. 1978. 135: 603-611
pfkB phosphofructokinase phosphofructokinase, Pfk-2 unknown minimal medium 63 (J. Biol. Chem. 1964. 239:2765-2771) supplemented with 1 % tryptic digest (BBL) and 0.4 % yeast extract (Difco) L broth •in pfkA-: grow on mannitol (4 mg/ml) Morrissey, A. T. E. & D. G. Fraenkel
J. Bacteriol. 1972. 112: 183-187
pfl pyruvate formate-lyase give white colonies by overlaid soft agar (see Notes) (wild type: purple colonies) rich broth containing (per liter) 10 g of tryptone, 5 g of NaCl, 1 g of yeast extract L broth •soft agar (5 ml) is containing 2.5 ml of 1.5 % agar, 1.37 ml of 10 % sodium pyruvate, 1 ml of 0.5 % benzyl viologen, and 0.125 ml of 1 M potassium phosphate buffer (pH 7.0) Chang, Y.-Y. & J. E. Cronan, Jr.
J. Bacteriol. 1982. 151: 1279-1289
pgi phosphoglucose isomerase glucosephosphate isomerase grow slowly on glucose (0.4 %) minimal medium 63(J. Biol. Chem. 1964. 239: 2765-2771) supplemented with 1 % tryptone (Difco) and 0.4 % glucose L broth Fraenkel, D. G. & S. R. Levisohn
J. Bacteriol. 1967. 93: 1571-1578
pgk phosphoglycerate kinase phosphoglycerate kinase unable to grow on glucose (10 mM), glycerol (25 mM), or succinate (40 mM) as a carbon source basal medium containing 0.58 % Na2HPO4, 0.3 % KH2PO4, 0.1 % NH4Cl, 0.05 % Nacl, and 0.01 % MgSO4 supplemented with 1 μg/ml thiamine hydrochloride, 0.5 % tryptone, 40 mM sodium succinate, and 4 mM glycerol L broth Irani, M. & P. K. Maitra
Biochem. Biophys. Res. Commun. 1974. 56: 127-133
pgl phosphogluconnalactonase blu 6-phosphogluconolactonase when grown on plates containing maltose, turn blue after iodine treatment (flooded with 4 ml of solution containing 0.01 M I2 and 0.03 M KI) minimal medium 63(J. Biol. Chem. 1964. 239: 2765-2771) supplemented with 1 % tryptic digest and 0.4 % yeast extract (Difco) L broth Kupor, S. R. and D. G. Fraenkel
J. Bacteriol. 1969. 100: 1296-1301
pgsA phosphotidylglycerophosphate synthase phosphotidylglycerophosphate synthase unknown LB broth (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) containing (per liter) 10 g of NaCl, 10 g of tryptone (Difco), and 5 g of yeast extract (Difco) L broth Nishijima, M. & R. H. Raetz
J. Biol. Chem. 1979. 254: 7837-7844
pheA phenylalanine chorismate mutase-P-prephenate dehydratase require phenylalanine (10-4M) medium A (Davis, B. D. & E. S. Mingioli. J. Bacteriol. 1950. 60:17-28) supplemented with phenylalanine (10-4M) L broth •resistant to ortho-fluorophenylalanine(1 mM) or para-fluorophenylalanine(10 mM)(J. Bacteriol. 1971. 106: 784-790 ) Brown, K. D.
Genetics. 1968. 60: 31-48
pheS phenylalanine phe-act phenylalanyl-tRNA synthase alpha-subunit resistant to p-fluorophenylalanine (6 mM) enriched media containing 20 % Solution P (1.34 % Na2HPO4•H2O, 1.34 % KH2PO4, 0.0011 % CaCl2, and 0.0246 % MgSO4•H2O), 1% tryptone, 0.5 % yeasst extract, and 0.4 % glucose L broth •sensitive to nonpermissive temperature (37 ° C) (J. Bacteriol. 1965. 89: 706-711) Fangman, W. L. & F. C. Neidhardt
J. Biol. Chem. 1964. 239: 1839-1843
phoA phosphate alkaline phosphatase form white colonies on plates containing 5-bromo-4-chloro-3-indolyl-phosphate-p-toluidine (2x10-3%) and glucose (0.2 %) at low phosphate concentration (10-4 M) LB L broth Wanner, B. L. & P. Latterell
Genetics. 1980. 96: 353-366
phoE phosphate ompE outer membrane porin protein E resistant to phage TC45 yeast broth (Mol. Gen. Genet. 1976. 147: 251-262) L broth Tommassen, J. & B. Lugtenberg
J. Bacteriol. 1981. 147: 118-123
phoR phosphate nmpB phoR1 R1pho positive and negative regulatory gene for pho regulon form blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-phosphate-p-toluidine (2x10-3 %) and glucose (0.2 %) at high phosphate concentration (2x10-3 M) LB L broth Wanner, B. L. & P. Latterell
Genetics. 1980. 96: 353-366
phr photoreactivation phrB deoxyribodipyrimidine photolyase fail to photoactivate (by exposure to light for 50 min) after UV irradiation LB or K medium (J. Mol. Biol. 1971. 61: 25-44) L broth Sancar, A. & C. S. Rupert
Gene. 1978: 295-308
pit Pi transport low-affinity Pi transport require DL-glycerol-3-phosphate (0.04 %) medium E (vogel and bonner 1956) supplemented with 0.4 % carbon sources and DL-glycerol-3-phosphate (0.04 %) or LB L broth •resistant to arsenate (20 mM) Sprague, G. F. et al.
Mol. Gen. Genet. 1975. 143. 71-77
plsB phospholipid synthesis glycerophosphate acyltransferase activity unknown minimal glucose, maltose medium, or LB medium L broth •in plsX-: require glycerol (0.4 %) or sn-glycerol-3-phosphate (0.2 %) Larson, T. J. et al.
J. Bacteriol. 1984. 160: 711-717
plsX phospholipid synthesis glycerol P auxotrophy in plsB background unknown minimal glucose, maltose medium, or LB medium L broth •in plsB-: require glycerol (0.4 %) or sn-glycerol-3-phosphate (0.2 %) Larson, T. J. et al.
J. Bacteriol. 1984. 160: 711-717
pnc pyridine nucleotide cycle nam nicotinamide deaminase resistant to 6-aminonicotinamide (2 mM) nutrient (tryptone-yeast) medium containig per liter 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl L broth •in nicotinic acid auxotroph (i.e. nad-): require nicotinic acid but unable to grow on nicotinamide (0.1 mM) White, B. J. et al.
J. Bacteriol. 1976. 126: 1082-1088
pnp polynucleotide phosphorylase polynucleotide phosphorylase unknown yeast-tryptone (YT) broth containing 5 g of NaCl, 8 g of tryptone (Difco), and 5 g of yeast extract per liter L broth Reiner, A. M.
J. Bacteriol. 1969. 97: 1431-1436
polA polymerase resA DNA polymerase I sensitive to methylmethanesuphonate (0.04 %) Penassay broth L broth •sensitive to UV De Lucia, P. & J. Cairns
Nature. 1969. 224: 1164-1166
polB polymerase dinA DNA polymerase II sensitive to H2O2 (20 mM) LB medium L broth •slightly more sensitive to nitrogen mustard (J. Bacteriol. 1999. 181: 2878-2882) Escarceller, M. et al.
J. Bacteriol. 1994. 176: 6221-6228
poxB pyruvate oxidase pyruvate oxidase unknown rich broth containing 10 g of tryptone, 5 g of NaCl, 1 g of yeast extract (J. Bacteriol. 1982. 151: 1279-1289) L broth •in aceEF-: require acetate (10 mM) Chang, Y.-Y. & J. E. Cronan, Jr.
J. Bacteriol. 1983. 154: 756-762
pps PEP synthase ppsA phosphoenolpyruvate synthase unable to grow on pyruvate or lactate (50 mM) as the sole carbon source 'Oxyoid' nutrient agar L broth Cooper, R. A. & H. L. Kornberg, F. R. S.
Proc. R. Soc. Lond. B. Biol. Sci. 1967. 168: 263-280
proA proline pro1 gamma-glutamyl phosphate reductase require proline (40 μg/ml) Penassay Broth L broth •resistant to 4-nitropyridine-N-oxide (1 to 3 μg/ml) Hirota, S. et al.
J. Bacteriol. 1966. 91: 2392
proB proline pro2 gamma-glutamyl phosphate kinase require proline (40 μg/ml) Penassay Broth L broth •resistant to 4-nitropyridine-N-oxide (1 to 3 μg/ml) Hirota, S. et al.
J. Bacteriol. 1966. 91: 2392
proC proline pro2 pro3 pyrroline-5-carboxylate reductase require proline (0.1 mg/ml) inorganic salts medium supplemented with 0.5 % glucose and proline (0.1 mg/ml) L broth Baich, A. & D. J. Pierson
Biochim. Biophys. Acta. 1965. 104: 397-404
proS proline drp prolyl-tRNA synthetase sensitive to nonpermissive temperature (42 °C) LB + M9 supplemented with phosphate buffer and NH4Cl L broth Bohman, K. & L. A. Isaksson
Mol. Gen. Genet. 1980. 177: 603-605
prpB propionate 2-methylisocitrate lyase unknown (no mutant yet) Brock, et al.
Eur. J. Biochem. 2001. 268: 3577-3586
prpC propionate 2-methylcitrate synthase unknown (no mutant yet) Texter, S. & V. F. Wendisch et al.
Arch. Microbiol. 1997. 168: 428-436
prpD propionate yahT acnC 2-methylcitrate dehydrogenase, PrpD unknown L broth (Lennox, E. S. 1955. Virology. 1: 190-206) L broth Blank, L. et al.
Microbiology. 2002. 148: 133-146
prpE propionate unknown (no mutant yet) Texter, S. & V. F. Wendisch et al.
Arch Microbiol. 1997. 168: 428-436
prpR propionate regulation of prpB operon unknown (no mutant yet)
psd phosphatidylserine decarboxylase phosphatidylserine decarboxylase sensitive to nonpermissive temperature (42 °C ) L-broth L broth Hawrot, E & E. P. Kennedy
Proc. Natl. Acad. Sci. USA. 1975. 72: 1112-1116
psu pleiotropic suppressor resistant to oxolinic acid (1.6 - 3.2 μg/ml) Luria broth (LB) L broth Ephrati-Elizur, E.
J. Bacteriol. 1993. 175: 207-213
ptsG phosphotransferase system CR car cat gpt umg glucosephosphotransferase enzyme II form blue coloies on BCIG plates ML broth containing per liter 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl L broth •form light red colonies on glucose-MacConkey plates (WT: red colonies) Curtis, S. J. & W. Epstein
J. Bacteriol. 1975. 122: 1189-1199
ptsH phosphotransferase system Hpr ctr hpr phosphohistidinoprotein-hexose phosphotransferase form dark-red colony on peptone-tetrazolium (TP) plate ML broth containing per liter 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl L broth Epstein, W. et al.
J. Bacteriol. 1970. 104: 793-797
ptsI phosphotransferase system ctr phosphotransferase system enzyme I form dark-red colony on peptone-tetrazolium (TP) plate ML broth containing per liter 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl L broth Epstein, W. et al.
J. Bacteriol. 1970. 104: 793-797
purA purine adenylosuccinate synthetase require adenine (20 μg/ml) minimal medium (J. Bacteriol. 1950. 60:17-28) supplemented with glucose (2 mg/ml) and adenine (20 μg/ml) or nutrient broth (Oxoid CM67) L broth supplemented with adenine (20 μg/ml) •sensitive to 6-methylpurine (3 mM) but resistant to 6-methylpurine + hypoxanthine (0.6 mM) (J. Bacteriol. 1970. 101: 872-880) Mehra, R. K. & W. T. Drabble
J. Gen. Microbiol. 1981. 123: 27-37
purB purine adenylosuccinate lyase require adenine (0.15 mM) minimal medium of ammonium salts and glucose (J. Bacteriol. 1966. 91: 1037-1049) supplemented with casein hydrolysate (0.1 %) and adenine (0.15 mM) L broth supplemented with adenine (0.15 mM) •resistant to nalidixic acid (4 μg/ml)(J. Bacteriol. 1970. 1027-1029) Benson, C. E. et al.
J. Bacteriol. 1970. 101: 872-880
purC purine adeg phosphoribosylaminoimidazolesuccinocarboxamide synthetase require adenine (20 μg/ml) L-broth supplemented with 0.2 % (wt/vol) glucose or 0.2 % maltose L broth supplemented with adenine (20 μg/ml) Parker J.
J. Bacteriol. 1984. 157: 712-717
purE purine Pur2, ade3, adef, purE1 phosphoribosylaminoimidazole carboxylase, catalytic subunit require purines (20 μg/ml) L-broth (Miller. 1972. J. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) L broth supplemented with purines (20 μg/ml) Kamholz, J.
Gene. 1986. 44: 55-62
purF purine adeub purC amidophosphoribosyl transferase require purines (50 μg/ml) L-broth L broth supplemented with purines (50 μg/ml) Smith, J. M. & J. S. Gots
J. Bacteriol. 1980. 143: 1156-1164
purH purine phosphoribosylaminoimidazolecarboxamide formyltransferase require adenine (10 μg/ml) LB L broth supplemented with adenine (10 μg/ml) Aiba, A. & K. Mizobuchi
J. Biol. Chem. 1989. 264: 21239-21246
purL purine purI phosphoribosylformylglycineamide synthetase require adenine (10 μg/ml) LB L broth supplemented with adenine (10 μg/ml) Sampei, G. & K. Mizobushi
J. Biol. Chem. 1989. 264: 21230-21238
purM purine purG phosphoribosylaminoimidazole synthetase require purines (20 μg/ml) M9 supplemented with purines (20 μg/ml) L broth supplemented with purines (20 μg/ml) Parker, J. & S. E. Fishman
J. Bacteriol. 1979. 138: 264-267
putA proline utilization proline dehydogenase unkown LB medium L broth •in putP-: resistant to azetidine carboxylate (25 μg/ml) Wood, J. M.
J. Bacteriol. 1981. 146: 895-901
putP proline utilization proline/Na+, Li+ symport protein resistant to dehydroproline (10 μg/ml) and azetidine carboxylate (25 μg/ml) LB medium L broth •in putA -: resistant only to azetidine carboxylate(25μg/ml) Wood, J. M.
J. Bacteriol. 1981. 146: 895-901
pyrB pyrimidine aspartate transcarbamylase, catalytic subunit require uracil (20 μg/ml) M63 medium containing per liter 2 g of (NH4)2SO4, 0.2 g of Mg SO4•H2O, 0.5 mg of FeSO4, and 0.1 M potassium phosphate supplemented with 0.2 % glycerol and 20 μg/ml of uracil L broth supplemented with uracil (20 μg/ml) Beckwith, J. R. et al.
J. Mol. Biol. 1962. 5: 618-634
pyrC pyrimidine dihydroorotase require uracil (20 μg/ml) M63 medium containing per liter 2 g of (NH4)2SO4, 0.2 g of Mg SO4•H2O, 0.5 mg of FeSO4, and 0.1 M potassium phosphate supplemented with 0.2 % glycerol and 20 μg/ml of uracil L broth supplemented with uracil (20 μg/ml) Beckwith, J. R. et al.
J. Mol. Biol. 1962. 5: 618-634
pyrD pyrimidine dihydroorotate dehydrogenase require uracil (20 μg/ml) M63 medium containing per liter 2 g of (NH4)2SO4, 0.2 g of MgSO4•H2O, 0.5 mg of FeSO4, and 0.1 M potassium phosphate supplemented with 0.2 % glycerol and 20 μg/ml of uracil L broth supplemented with uracil (20 μg/ml) Beckwith, J. R. et al.
J. Mol. Biol. 1962. 5: 618-634
pyrE pyrimidine orotate phosphoribosyltransferase require uracil (20 μg/ml) M63 medium containing per liter 2 g of (NH4)2SO4, 0.2 g of Mg SO4•H2O, 0.5 mg of FeSO4, and 0.1 M potassium phosphate supplemented with 0.2 % glycerol and 20 μg/ml of uracil L broth supplemented with uracil (20 μg/ml) Beckwith, J. R. et al.
J. Mol. Biol. 1962. 5: 618-634
pyrF pyrimidine orotidine-5'-phosphate decarboxylase require uracil (20 μg/ml) M63 medium containing per liter 2 g of (NH4)2SO4, 0.2 g of Mg SO4•H2O, 0.5 mg of FeSO4, and 0.1 M potassium phosphate supplemented with 0.2 % glycerol and 20 μg/ml of uracil L broth supplemented with uracil (20 μg/ml) Beckwith, J. R. et al.
J. Mol. Biol. 1962. 5: 618-634
pyrH pyrimidine smbA UMP kinase sensitive to cold temperature (22 °C) L medium L broth •sensitive to SDS •in mukB-: resistant to nonpermissive temperature (42 ° C) (suppress mukB phenotype Yamanaka, K. et al.
J. Bacteriol. 1992. 174: 7517-7526
rac recombination activation (defective prophage Rac loci) unknown Luria broth L broth Low, B.
Mol. Gen. Genet. 1973. 122: 119-130
ranA RNA metabolism unknown sensitive to nonpermissive temperature (45 °C) in enriched medium enriched liquid medium containing 8 g of nutrient broth and 8 g of technical Casamino Acids per liter or liquid minimal medium containing only inorganic salts and glucose (J. Mol. Biol. 1966. 16: 285-301) L broth Apirion, D. & N. Watson
J. Bacteriol. 1975. 124: 317-324
rbs ribose ribokinase unable to grow on ribose as sole carbon source Oxoid nutrient broth L broth Anderson, A. & R. A. Cooper
J. Gen. Microbiol. 1970. 62: 335-339
rcsF regulation capsule (colanic acid) synthesis regulatory gene for expolysaccharide synthesis: colanic acid biosynthesis unknown L broth containing 1.0 % tryptone (NZ-Amine A; ICN Biochemicals Inc.), 0.5 % yeast extract, and 0.5 % NaCl L broth Gervais, F. G. & G. R. Drapeau
J. Bacteriol. 1992. 174: 8016-8022
recA recombination srf lexB umuB zab general recombinatio and DNA repair; pairing and strand exchange; role in cleavage of LexA repressor, SOS mutagenesis sensitive to UV complex broth comtaining 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl per liter L broth •recA200: sensitive to UV at nonpermissive temperature (43 ° C) (J. Bacteriol. 1974. 120: 407-415)
•sensitive to methyl methanesulfonate (0.1 %), mitomycin C (1 μg/ml), phleomycin (0.5 μg/ml), and hydxyurea (10 mM) (J. Bacteriol. 2002. 184: 6836-6844) Clark, A. J. & A. D. Margulies
Proc. Natl. Acad. Sci. USA. 1965. 53: 451-459
recB recombination rorA RecBCD enzyme (exonuclease V) subunit sensitive to UV Penassay broth (Difco Antibiotic Medium 3) or L broth L broth •sensitive to mitomycin C (1 μg/ml), phleomycin (0.5 μg/ml), and hydxyurea (10 mM) (J. Bacteriol. 2002. 184: 6836-6844) Ganesan, A. K. & K. C. Smith
J. Bacteriol. 1968. 96: 365-373
recC recombination RecBCD enzyme (exonuclease V) subunit sensitive to UV Penassay broth (Difco Antibiotic Medium 3) or L broth L broth Ganesan, A. K. & K. C. Smith
J. Bacteriol. 1968. 96: 365-373
recD recombination hopE RecBCD enzyme(exonuclease V) form indistinguishable large plaques by Chi+ and Chi0 Red- Gam- lambda phage LB medium or tryptone broth L broth •increase amount of small plasmids (J. Bacteriol. 1987. 169:4841-4844) Russell, C. B. et al.
J. Bacteriol. 1989. 171: 2609-2613
recE recombination exonuclease VII unknown Luria broth (J. Bacteriol. 1969. 97: 244-249) L broth Fouts, K. E. et al.
J. Bacteriol. 1983. 156: 718-726
recN recombination radB LexA regulon; recombination and repair sensitive to mitomycin C (2 μg/ml) and gamma radiation Luria broth L broth Picksley, S. M. et al.
Mol. Gen. Genet. 1984. 195: 267-274
recO recombination RecA-like strand assimilation sensitive to UV L broth L broth Kolodner, R. et al.
J. Bacteriol. 1985. 163: 1060-1066
recQ recombination RecQ helicase sensitive to phleomycin (0.5 μg/ml) Luria-Bertani (LB) medium containing 1 % of Bacto Tryptone, 0.5 % yeast extract, and 0.5 % NaCl L broth •in thyA-: resistant to thymineless death (Mol. Gen. Genet. 1984. 195: 474-480)
•in recBC- sbcB-: sensitive to UV (Mol. Gen. Genet. 1984. 195: 474-480) Beam, C. E. et al.
J. Bacteriol. 2002. 184: 6836-6844
relA relax rel unknown resistant to near UV (300 to 380 nm) M9 minimal medium L broth •sensitive to nonpermissive temperature (42 ° C) (J. Bacteriol. 2003. 185: 5765-5771) •in rpoB-: resistant to nonpermissive temperature (suppress Ts phenotype)(J. Bacteriol. 2003. 185: 5765-5771) Ramabhadran, T. V.
J. Bacteriol. 1976. 127: 1587-1589
relB relax unknown unable to recover for about 3 h following release from 1 h isoleucine starvation (minimal medium without all amino acids but isoleucine (800 μg/ml)) broth containing per liter 1 g of glucose, 10 g of bactortyptone, 5 g of yeast extract, 5 g of NaCl, 0.3 g of KH2PO4 and 0.7 g of K2HPO4 L broth Diderichsen, B. & L. Desmarez
Mol. Gen. Genet. 1980. 180: 429-437
rep replicase dasC mbrA mmrA Rep helicase resistant to phiX174 TKCaB containing per liter 10 g of Bacto-Tryptone (Difco), 5 g of KCl, amd 0.015 M CaCl2 or tryptone broth containing per liter 10 g of tryptone, 5 g of NaCl, and 0.01 M MgSO4 L broth •sensitive to UV
•instable to lysogenize lambda Denhardt, D. T. et al.
Proc. Natl. Acad. Sci. USA. 1967. 57: 813-820
rfa rough con lps phx sensitive to novobiocin (< 40 μg/ml) LB broth L broth •unable to grow on eosin-methylelne blue plates or on plates containing 0.1 % deoxycholate
•resistant to phage K20 and phage U3 Austin, E. A. et al.
J. Bacteriol. 1990. 172: 5312-5325
rfbC rough rfbD dTDP-4-deoxyrhamnose-3,5-epimerase unknown L broth (constituents from Difco) L broth Stevenson, G. et al.
J. Bacteriol. 1994. 176: 4144-4156
rhaA rhamnose rhamnose isomerase form white colonies on Endo agar plates containing 1 % L-rhamnose nutrient broth (Difco) L broth Power, J.
Genetics. 1967. 55: 557-568
rhaB rhamnose rhamnulokinase form white colonies on Endo agar plates containing 1 % L-rhamnose nutrient broth (Difco) L broth Power, J.
Genetics. 1967. 55: 557-568
rhaD rhamnose rhamnulosephosphate aldolose D form white colonies on Endo agar plates containing 1 % L-rhamnose nutrient broth (Difco) L broth •sensitive to L-rhamnose (0.2 %) in 0.15 % casein hydrolysate mineral medium Power, J.
Genetics. 1967. 55: 557-568
rhaR rhamnose rhaC positive regulatory gene form white colonies on Endo agar plates containing 1 % L-rhamnose nutrient broth (Difco) L broth Power, J.
Genetics. 1967. 55: 557-568
rhaS rhamnose rhaC positive regulatory gene form white colonies on Endo agar plates containing 1 % L-rhamnose nutrient broth (Difco) L broth Power, J.
Genetics. 1967. 55: 557-568
rhaT rhamnose rhamnose permease unable to grow on L-rhamnose (10 mM) as sole carbon source minimal medium supplemented with glycerol (20 mM) L broth Tate, C. G. et al.
J. Biol. Chem. 1992. 267: 6923-6932
rho Rho termination factor nitA nusD psuA rnsC sun tsu transcription termination factor Rho sensitive to nonpermissive temperature (42 °C) tryptone broth or L broth L broth •sensitive to UV
•resistant to phage lambda susN7nin5 but sensitive to lambda spi (Mol. Gen. Genet. 1976. 143: 211-221) Das, A. et al.
Proc. Natl. Acad. Sci. USA. 1976. 73: 1959-1963
rhsC recombination hotspot unknown minimal medium (J. Biol. Chem. 1956. 218: 97-106) supplemented with glucose (0.5 %) and Difco certified casamino acids (0.1 %) L broth Sadosky, A. B. et al.
J. Bacteriol. 1989. 171: 636-642
rna RNaseA rnsA RNase I unknown nutrient broth, Casamino acids or Casamino acids glucose medium (compositions described in Notes) L broth •nutrient broth containing per liter10 g of Bactotryptone (Difco), 1 g of yeast extract (Difco), 8 g of NaCl and 2 mM of CaCl2,
•Casamino acids glycerol medium containing per liter 5 g of Casamino acids (Difco), 5 g of NaCl, 1 g of NH4Cl, 4 ml of glycerol, 0.1 mM of Na2HPO4, 10 microM of FeCl3, 1 mM of MgSO4, 1.21 g of tris (Sigma 121) and 2 mM of CaCl2
•Casamino acids glucose medium containing per liter 2 g of Casamino acids (Difco, vitamine-free), 1 g of NaCl, 2 g of dextrose, 2 g of (NH4)2SO4, 20 ml of 1 M potassium phosphate buffer (pH 6.8) and 0.5 ml of salt solution containing 32 of MgCl2, 0.6 g of CaCl2 and 0.2 g of Fe2(SO4)3 in 100 ml Gessteland, R. F.
J. Mol. Biol. 1966. 16: 67-84
rnc RNase C RNase III give clear plaques by phage lambda or lambda imm434 LB L broth •make increased level of polynucleotide phosphorylase
•altered sizes of rRNA produced determined by gel electrophoresis Takiff, H. E. et al.
J. Bacteriol. 1989. 171: 2581-2590
rnhA RNase H cer dasF herA rnh sdrA sin RNase HI sensitive to rich media (e. g. L broth) M9 salts/glucose M9 medium supplemented with glucose •in dnaA-: resistatnt to nonpermissive temperature (42 ° C) Ogawa, T. et al.
Proc. Natl. Acad. Sci. USA. 1984. 81: 1040-1044
rnhB RNaseH RNase HII unknown (no mutant yet) L broth Itaya, M.
Proc. Natl. Acad. Sci. USA. 1990. 87: 8587-8591
rnpA RNaseP RNase P, protein component sensitive to nonpermissive temperature (43 °C) low phosphate peptone medium containing 10 g of peptone and 5 g of glucose per liter L broth Apirion, D.
Genetics. 1980. 94: 291-299
rpoB RNA polymerase ftsR groN mbrD nitB rif ron stl stv tabB sdgB RNA polymerase, beta-subunit resistant to rifanpicin (50 μg/ml) LB L broth Jin, D. J. & C. A. Gross
J. Mol. Biol. 1988. 202: 45-58
rpoN RNA polymerase glnF ntrA RNA polymerase sigma60-subunit require glutamine (0.2 %) LB L broth glutamine (0.2 %) •in relA-: require glutamine (0.2 %) even in LB
•in ftsZ-: resistant to nonpermissive temperature(42 ° C) without glutamine supplementation in LB Powell, B. S. & D. L. Court
J. Bacteriol. 1998. 180: 1053-1062
rpoS RNA polymerase abrD dpeB katF mur appR RNA polymerase sigma, stationary phase fail to evolve bubbles of oxygen with a drop of 30 % H2O2 on LB plate LB medium containing 10 g of tryptone (Difco), 5 g of yeast extract (Difco), and 10 g of NaCl per liter L broth Loewen, P. C. & B. L. Triggs
J. Bacteriol. 1984. 160: 668-675
rpsB ribosomal protein, small 30S ribosomal subunit protein S2 sensitive to nonpermissive temperature (42 °C) TA containing tryptone (1 %), NaCl (0.5 %), and vitamine B1 (1 mg/l) L broth Bollen, A. et al.
J. Mol. Biol. 1979. 132: 219-233
rpsE ribosomal protein, small eps spc spcA 30S ribosomal subunit protein S5 resistant to spectinomycin (100 μg/ml) TY medium (J. Mol. Biol. 1969. 46: 73-84) L broth •Some rpsE mutants require 20 % sucrose for resistance to spectinomycin. Miyoshi, Y. & H. Yamagata
J. Bacteriol. 1976. 125: 142-148
rpsL ribosomal protein, small asuB strA 30S ribosomal subunit protein S12 resistant to streptomycin (500 μg/ml) nutrient broth L broth Skavronskaya, A. G. et al.
Mutat. Res. 1973. 19: 49-56
rrfH rRNA large, 23S 5S rRNA of rrnH operon unknown (no mutant yet)
rrlH rRNA large, 23S 23S rRNA of rrnH operon unknown (no mutant yet)
rrsH rRNA large, 23S 16S rRNA of rrnH operon unknown (no mutant yet)
rtcA RNA terminal P cyclase RnA 3'-terminal phosphate cyclase unknown (no mutant yet) Genschik, P. et al.
J. Biol. Chem. 1998. 273: 25516-25526
rtcB RNA terminal P cyclase unknown (no mutant yet) Genschik, P. et al.
J. Biol. Chem. 1998. 273: 25516-25526
ruvA repair UV ? (lexA regulon; Holiday junction recognition) sensitive to UV L broth containing (per liter) 5 g of yeast extract, 10 g of polypeptone, 5 g of NaCl, and 1 g of glucose, pH 7.2 L broth •sensitive to mitomycin C (0.5 μg/ml) Otsuji, N. et al.
J. Bacteriol. 1974. 117: 337-344
ruvB repair UV ? (lexA regulon; branch migration of Holicay junction) sensitive to UV L broth containing (per liter) 5 g of yeast extract, 10 g of polypeptone, 5 g of NaCl, and 1 g of glucose, pH 7.2 L broth •sensitive to mitomycin C (0.5 μg/ml) Otsuji, N. et al.
J. Bacteriol. 1974. 337-344
ruvC repair UV ? RuvC endonuclease sensitive to UV LB broth (J. Bacteriol. 1974. 120: 407-415) L broth •sensitive to mitomycin C (0.2 and 0.5 μg/ml) Sharples, G. J. et al.
Mol. Gen. Genet. 1990. 221: 219-226
sad succinate-semialdehyde deHase succinate-semialdehyde dehydrogenase, NAD dependent unable to grow on 2.5 mM succinic semialsehyde LB (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth Marek, L. E. & J. M. Henson
J. Bacteriol. 1988. 170: 991-994
sbcB suppression of recBC xonA exonuclease I unknown L broth L broth •in recB- recC-: resistant to UV, resistant to mitomycin C (1 μg/ml) (suppress recBC- phenotypes) Kushner, S. R. et al.
Proc. Natl. Acad. Sci. USA. 1972. 69: 1366-1370
sdh succinate dehydrogenase unable to grow on succinate (0.025 M) basal medium containing per liter 70 g of K2HPO4, 30 g of KH2PO4, 1.0 g of (NH4)2SO4, 0.25 g of MgSO4•H2O supplemented with 0.025 M of glucose L broth Hirsch, C. A. et al.
J. Biol. Chem. 1963. 238: 3770-3774
secA secretory azi pea prlD translocation ATPase for protein export resistant to phenethyl alcohol (0.23-24 % for liquid medium, 0.22-0.23 % for agar medium) minimal medium E (Vogel, H. J., and D. M. Bonner. J. Biol. Chem. 1956. 218: 97-106) with glucose or peptone-glucose medium containing 20 g of polypeptone (Wako Drug Co.), 5 g of NaCl, and 2 g of glucose per liter L broth •resistant to sodium azide (3.4 mM) Yura, T., & C. Wada
Genetics. 1968. 59: 177-190
secY secretory prlA multispanning membrane protein, translocator of proteins unknown LB broth L broth •in lamB-: sensitive to phage lambda, grow on maltodextrins (suppress LamB- phenotypes) Emr, S. D. et al.
Cell. 1981. 23: 79-88
selD selenium fdhB selenophosphate synthase unknown rich medium containing 1% tryptone, 0.5 % yeast extract, L broth Leinfelder, W. et al.
J. Bacteriol. 1988. 170: 540-546
serA serine phosphoglycerate dehydrogenase require serine (40 mg/l) L broth L broth •grow on elevated lavels of leucine, arginine, lysine, threonine, and methionine (each 240 mg/l) Ravnikar, P. D. & R. L. Somerville
J. Bacteriol. 1987. 169: 2611-2617
serB serine phosphoserine phosphatase require serine (40 mg/l) L broth L broth •grow on elevated lavels of leucine, arginine, lysine, threonine, and methionine (each 240 mg/l) Ravnikar, P. D. & R. L. Somerville
J. Bacteriol. 1987. 169: 2611-2617
serC serine pdxC pdxF phosphoserine aminotransferase require pyridoxine (0.1 mg/liter) and serine (300 mg/liter) glucose minimal medium (J. Bacteriol. 1969. 97: 1403-1410) supplemented with pyridoxine (0.1 mg/liter) and serine (300 mg/liter) L broth •grow on elevated lavels of leucine, arginine, lysine, threonine, and methionine (each 240 mg/l) (J. Bacteriol. 1987. 169: 2611-2617) Dempsey, W. B.
J. Bacteriol. 1969. 100: 1114-1115
serR serine unknown Luria and Burrous (LB) medium L broth •suppress Ts phenotype Theall, G. et al.
Mol. Gen. Genet. 1979. 169: 205-211
serS serine seryl-tRNA synthetase resistant to L-serine hydroxamate (1.28 mM) minimal medium supplemented with glucose (2 mg/ml) L broth Tosa, T. & L. I. Pizer
J. Bacteriol. 1971. 106: 972-982
serU serine su Su-1 ftsM supD supH serine tRNA2 unknown LG broth containing 10 g of tryptone, 5 g of yeast extract, 5 g of NaCl, and 5 g of glycerol per liter L broth •in case of supD: suppress missense mutation (amber) Steege, D. A. & J. I. Horabin
J. Bacteriol. 1983. 155: 1417-1425
serV serine supD serine tRNA3 unknown LB broth (J. Bacteriol. 1957. 74: 461-476) L broth •in case of supD: suppress missense mutation (amber) Steege, D. A. & B. Low
J. Bacteriol. 1975. 122: 120-128
sfa (chromosomal feature rather than a gene per se in F+ strains) sex factor affinity unknown broth (Luria, S. E. et. al. 1957. J. Bacteriol. 74: 461-476) L broth Aderlberg, E. A. & S. H. Burns
J. Bacteriol. 1960. 79: 321-330
sfiC septum fromation inhibition element of e14, inhibitor of cell division unknown LB broth L broth •in recA441(Tif) sulA-: form uniformly small cells after incubation for 3 hours at 42 ° C D'ari, R. & O. Huisman
J. Bacteriol. 1983. 156: 243-250
sloB slow growth unknown tolerant to nalidixic acid (7 μg/ml) or amidino penicillin (10 μg/ml) LB (J. Bacteriol. 1951. 62:293-300) supplemented with medium E (J. Biol. Chem. 1956. 218: 97-106) and 0.2 % glucose L broth •grow slowly Westling-Haggstrom, B. & S. Normark
J. Bacteriol. 1975. 123: 75-82
sodA SOD, superoxide dismutase superoxide dismutase, Mn sensitive to paraquat (3x10-7 M) in minimal medium LB L broth Carlioz, A & D. Touati
EMBO J. 1986. 5: 623-630
sodB SOD, superoxide dismutase superoxide dismutase, Fe sensitive to paraquat (10-5 M) in minimal medium LB L broth Carlioz, A & D. Touati
EMBO J. 1986. 5: 623-630
speA spermidine biosynthetic decarboxylase require putrescine in the presence of arginine but not in its absence basal medium of Davis & Mingioli. (J. Bacteriol. 1950. 60:17-28) supplemented with 0.5 % glucose Maas, W. K.
Mol. Gen. Genet. 1972. 119: 1-9
speB spermidine agmatinase require putrescine in the presence of arginine but not in its absence basal medium of Davis & Mingioli. (J. Bacteriol. 1950. 60:17-28) supplemented with 0.5 % glucose Maas, W. K.
Mol. Gen. Genet. 1972. 119: 1-9
speD spermidine S-adenosylmethionine decarboxylase unknown defined medium (J. Biol. Chem. 1958. 218: 97-106) L broth Tabor, C. W. et al.
Proc. Natl. Acad. Sci. USA. 1986. 83: 6040-6044
speE spermidine spermidine synthase unknown defined medium (J. Biol. Chem. 1958. 218: 97-106) L broth Tabor, C. W. et al.
Proc. Natl. Acad. Sci. USA. 1986. 83: 6040-6044
spoT spot (magic spot) guanosine 5'-diphosphate, 3'-diphosphate pyrophosphatase unknown Trisglucose medium containing 0.1 M Tris-HCl pH 7.4, 422.5 mg/l of sodium citrate (4H2O), 100 ug/l of MgSO4, 0.405 mg/l of FeCl3, 1 g/l of (NH4)2SO4, 2 x 10-3 M potassium phosphate pH 7 and 0.2 % glucose L broth Laffler, T. & J. Gallant
Cell. 1974. 1: 27-30
sppA signal peptide peptidase protease IV, siganl peptide peptidase unknown LB (24Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) containing 10 g of tryptone (Difco), 5 g of yeast extract (Difco), and 5 go of NaCl per liter L broth Suzuki, T. et al.
J. Bacteriol. 1987. 169: 2523-2528
srlA sorbitol gutA D-glucitol-specific enzyme II of phosphotransferase system unable to grow on glucitol (0.2 %) L-broth L broth Csonka, L. N. & A. L. Clark
Genetics. 1979. 93: 321-343
srlB sorbitol gutB D-glucitol(sorbitol)-specific enzyme II of the phosphotransferase system unknown (no mutant yet) Yamada, M. & M. H. Saier, Jr.
J. Mol. Biol. 1987. 262: 5455-5463
srlD sorbitol gutD sorbitol-6-phosphate dehydrogenase sensitive to glucitol (0.2 %) L-broth L broth Csonka, L. N. & A. L. Clark
Genetics. 1979. 93: 321-343
srlE sorbitol gutE protein encoded by reading frame adjacent to srlA unknown (no mutant yet)
srlR sorbitol gutR unknown L-broth L broth •in mtl-: grow on mannitol (0.2 %) Csonka, L. N. & A. L. Clark
Genetics. 1979. 93: 321-343
ssb single-stranded binding exrB lexC single-stranded DNA-binding protein sensitive to UV L broth-thymine containing per liter 5 g of NaCl, 5 g of yeast ectract, 10 g of tryptone, 1.1 ml of 1 M NaOH, and 50 mg of thymine L broth •restrict the growth of single-stranded phages and phage lambda at 42 ° C Glassberg, J. et al.
J. Bacteriol. 1979. 140: 14-19
ssyD suppression of secY sensitive to low temperature (25 to 30 °C) LB medium L broth •in secY24-: grow at 42 ° C (suppress SecY phenotype) Shiba, K. et al.
J. Bacteriol. 1986. 166: 849-856
strC streptomycin strB low-level streptomycin resistance; modifies ribosome structure resistant to streptomycin (5 μg/ml) L-broth containing 10 g of Difco Bacto Tryptone, 5 g of Difco Yeast Extract, 5 g of NaCl, 1 g of glucose, and 0.2 g of thymine per liter L broth Roberts, L. M. & E. C.
Genet. Res. 1970. 16: 359-365
sts starvation-temperature sensitivity altered RNase activity unable to recover on broth agar plate at 30 °C after starvation on phosphate-agar plate at 42 ° C for 3 days or longer broth medium containing 10 g of Difco nutrient broth and 10 g of Difco vitamin-free Casamino acids per liter L broth Lennette, E. T. & D. Apirion
J. Bacteriol. 1971. 108: 1322-1328
sucB succinate lys met dihydrolipoamide succinyltransferase, component of alpha-ketoglutarate deHase unable to grow on glucose (0.2 %) as a sole carbon source L-broth L broth •grow on glucose (0.2 %) supplemented with succinate (4 mM) or lysine + methionine
•grow anaerobically on glucose alone (0.2 %) Herbert, A. A. & J. R. Guest
Mol. Gen. Genet. 1969. 105: 182-190
sulA suppressor of lon sfiA inhibits cell division and ftsZ ring formation unknown LB medium containing per liter 5 g of Difco yeast extract, 10 g of Difco tryptone and 10 g of NaCl L broth •in lon-: resistant to UV (suppress lon phenotype) George, J. et al.
Mol. Gen. Genet. 1975. 140: 309-332
supQ suppressor uncharacterized suppressor unknown LB supplemented with glucose (0.1 %) L broth •in pheS-353: resistant to nonpermissive temperature (42 ° C) (suppress pheS-353 phenotype) Russell, R. R. B. & A. J. Pittard
J. Bacteriol. 1971. 107: 736-740
sus unknown (no data)
tap taxis protein methyl-accepting protein IV. peptide receptor unresponsive to dipeptide minimal A medium (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) supplemented with 1 mM MgSO4 and 0.2 % glucose L broth Manson, M. D. et al.
Nature. 1986. 321: 253-256
tar taxis, aspartate and repellants cheM chemotactic signal transducer protein II unresponsive to attractants (aspartate or maltose) or repellents (Ni(II) or Co(II)) tryptone broth L broth Springer, M. S. et al.
Proc. Natl. Acad. Sci. USA. 1977. 74: 3312-3316
tbpA thiamine binding protein unknown (no mutant yet) Hollenbach, A. D. et al.
Protein Expr. Purif. 2002. 25: 508-18
tdcB threonine dehydratase, catabolic tdc mukA threonine dehydratase unknown LB or TB medium L broth Goss, T. J. & P. Datta
J. Bacteriol. 1984. 158: 826-831
tdi transduction inhibition sensitive to UV nutrient broth or tryptone broth L broth •unable to give recombinants with DNA transduced by phage P1 or Mu, nor transformants after exposure to R factor DNA
•grow slower even on rich media Stacey, K. A. & P. Oliver
J. Gen. Microbiol. 1977. 98: 569-578
tdk thymidine kinase deoxythimidine kinase resistant to near UV after growth with 5-bromodeoxyuridine (50 μg/ml) peptone-glucose medium containing (per liter) 20 g of polypeptone (Wako Drug Co.) , 5 g of NaCl, and 2 g of glucose L broth Hiraga, S. et al.
Biochim. Biophys. Acta. 1967. 145: 41-51
tgt tRNA-guanine transglycosylase tRNA-guanine transglycosylase grow slightly faster L broth supplemented with thymine (50 μg/ml) L broth Noguchi, S. et al.
J. Biol. Chem. 1982. 257: 6544-6550
thi thiamine require thiamine (0.01 microM) minimal medium (Davis, B. D. & E. S. Mingioli. J. Bacteriol. 1950. 60:17-28) supplemented with thiamine (0.01 microM) L broth •resistant to pyrithiamine (10 microM or 1 mM)(J. Biochem. 1969. 65: 417-425 ) Kawasaki, T. et al.
J. Bacteriol. 1968. 95: 1483-1485
thrA threonine thrD Hs aspartokinase I-homoserine dehydrogenase I require threonine (0.3 mM) or homoserine plus diaminopimelate minimal medium supplemented with carbon source (glucose or fructose, 4 mg/ml) and threonine (0.3 mM) L broth Theze, J. et al.
J. Bacteriol. 1974. 117: 133-143
thrB threonine homoserine kinase require threonine (0.3 mM) minimal medium supplemented with carbon source (glucose or fructose, 4 mg/ml) and threonine (0.3 mM) L broth Theze, J. et al.
J. Bacteriol. 1974. 117: 133-143
thrC threonine threonine synthase require threonine (0.3 mM) minimal medium supplemented with carbon source (glucose or fructose, 4 mg/ml) and threonine (0.3 mM) L broth Theze, J. et al.
J. Bacteriol. 1974. 117: 133-143
thrS threonine autogenously regulated threonyl-tRNA synthetase resistant to borrelidin(13.9microM) rich medium containing 1 % tryptone, 0.5 % yeast extract, and 0.4 % glucose L broth Nass, G., & J. Thomale
FEBS Lett. 1974. 39: 182-186
thrW threonine threonine tRNA2 unknown (no mutant yet) "•\"thrX\" mutation (Mol. Gen. Genet. 1978. 162: 95-100) can be in thrW gene." Comer, M. M.
Mol. Gen. Genet. 1982. 187: 132-137
ths unknown (no data)
thyA thymine thymidylate synthetase require thymine (50 μg/ml) even in LB LB supplemented with thymine (50 μg/ml) L broth supplemented with thymine(50 μg/ml) •in alaS-: resistant to thymineless death (J. Bacteriol. 1968. 95:991-997)
•in deoC-: grow on low thymine (1-2 μg/ml) Hickson, I. D. et al.
Gene. 1982. 18: 257-260
tnaA tryptophanase ind tnaR tryptophabase sensitive to indole (10 μg/ml) + 5-methyltryptophan (50 μg/ml) L-broth containing 1 % tryptone (Difco), yeast extract (Difco), 0.5 % NaCl, and 0.1 % glucose L broth Yudkin, M. D.
J. Gen. Microbiol. 1976. 92: 125-132
tnaB tryptophanase trpP low-affinity Trp permease sensitive to indole (10 μg/ml) + 5-methyltryptophan (50 μg/ml) L-broth containing 1 % tryptone (Difco), yeast extract (Difco), 0.5 % NaCl, and 0.1 % glucose L broth Yudkin, M. D.
J. Gen. Microbiol. 1976. 92:125-132
tolC tolerance colE1-i mtcB ref• weeA toc(topoisomerase compensation) mukA (outer menbrane channel) resistant to colicinE1 L broth L broth •sensitive to acriflavin HCl (5 μg/ml) or sodium deoxycholate (0.05 %) Whitney, E. N.
Genetics. 1971. 67: 39-53
tonB T-one T1rec exbA unptake of chelated Fe and cyanocobalamin, energy transfer resistant to phage T1, phi80 and colicin B double strength of Nutrient broth (Difco, 0003) plus 5 mg/ml of sodium chloride L broth Davis, J. K. & P. Reeves
J. Bacteriol. 1975. 123: 96-101
topA topoisomerase supX topoisomerase I, omega protein I sensitive to methyl methanesulfonate (0.65 ml/l) Penassay (Difco) or LB L broth •slightly sensitive to UV Sternglanz, R. et al.
Proc. Natl. Acad. Sci. USA. 1981. 78: 2747-2751
topB topoisomerase mutR topoisomerase III unknown LB medium L broth •in Lac-: increase Lac+ revertant frequency Whoriskey, S. K. et al.
Genetics. 1991: 127: 21-30
tpx thiol peroxidase thiol peroxidase, thioredoxin-linked grow slower on LB plate containing t-butyl hydroperoxide (0.25 mM) Luria-Bertani (LB) medium L broth •grow slightly slower in LB medium
•grow slower on LB plate containing paraquat (0.125-0.25 mM) Cha, M.-K. et al.
J. Bacteriol. 1996. 178: 5610-5614
traA (mutation in F plasmid) transfer-deficient F-pilin resistant to male-specific phages (such as f1, f2, and Qbeta) L broth L broth Achtman, M. et al.
J. Bacteriol. 1972. 110: 831-842
traD (mutation in F plasmid) transfer-deficient inner membrane protein, TraD resistant to phage f2 L broth L broth Achtman, M. et al.
J. Bacteriol. 1972. 110: 831-842
trg taxis to ribose and galactose methyl-accepting chemotaxis protein•, ribose acceptor, fllagellar regulon unresponsive to galactose (0.05 mM) and ribose (0.1 mM) LB L broth Park, C. & G. L. Hazelbauer
J. Bacteriol. 1986. 167: 101-109
trkA transport K+ potassium transport inner membrane protein subunit unknown KML, a high-K version of ML medium, containing per liter 10 g of tryptone, 10 g of KCl, and 5 g of yeast extract •in kdpABC-, trkD- : require potassium (115 mM)
•in kdpA-, trkD- : require potassium (20 mM) Polarek, J. W. et al.
Meth. Enzymol. 1988. 157: 655-667
trkD transport K+ major constitutive K+ transport system unknown KML, a high-K version of ML medium, containing per liter 10 g of tryptone, 10 g of KCl, and 5 g of yeast extract •in kdpABC-, trkA- : require potassium (115 mM)
•in kdpA-, trkA- : require potassium (20 mM) Polarek, J. W. et al.
Meth. Enzymol. 1988. 157: 655-667
trmB tRNA methyltransferase tRNA (guanine-7)-methyltransferase reduce efficiency in the suppression of amber mutations carried by T4 or lambda phages complete medium containing 20 g/l of brain heart infusion (Difco) plus 10 μg/ml of thymine L broth •grow slowly even in LB broth Marinus, M. G. et al.
J. Bacteriol. 1975. 122: 257-265
trpA tryptophan tryptophan synthase subunit A require tryptophan or indole(0.1 mg/ml) minimal salts medium (J. Biol. Chem. 1956. 218: 97-106) supplemented with 0.1 % glucose and tryptophan (0.1 mg/ml) L broth •grow on indoleglycerol as well as tryptonphan (Genetics. 1967. 57: 95-105) Hardman, J. K. et al.
J. Biol. Chem. 1975. 250: 4634-4642
trpB tryptophan tryptophan synthase subunit B require tryptophan (40 μg/ml) L-broth L broth •unable to grow on indole Smith, O. H.
Genetics. 1967. 57: 95-105
trpC tryptophan bifunctional enzyme N-(5-phosphoribosyl) anthranilate isomerase, indole-3-glycerolphosphate synthetase require tryptophan (40 μg/ml) L-broth L broth •grown on indole as well as tryptophan (J. Biol. Chem. 1960. 235: 2051-2057) Smith, O. H.
Genetics. 1967. 57: 95-105
trpD tryptophan bifunctional enzyme anthranilate synthase component iii, phosphoribosyl anthranilate transferase require tryptophan (40 μg/ml) L-broth L broth •grown on indole as well as tryptophan (J. Biol. Chem. 1960. 235: 2051-2057) Smith, O. H.
Genetics. 1967. 57: 95-105
trpE tryptophan anth tryD tryo-4 anthranilate synthase component • resistant to 5-methyltryptophan (10 μg/ml) peptone-glucose medium containing per liter 20 g of polypeptone (Wako Drug Co.), 5 g of NaCl, 2 g of glucose, and 1 M NaOH (for adjusting the pH to 7.2) L broth resistant to 3-methyanthranilic acid (0.2 micro-mol/ml) (J. Bacteriol. 1970. 101: 209-217) Hiraga, S.
J. Mol. Biol. 1969. 30: 159-179
trpR tryptophan regulator of trp operon and aroH, sutogenously regulated tryptophan repressor protein resistant to 5-methyltryptphan (5x10-4 M) minimal medium (J. Biol. Chem. 1956. 218: 97-106) supplemented with 0.5 % glucose or L-broth L broth Held, W. A. & O. H. Smith
J. Bacteriol. 1970. 101: 209-217
trpS tryptophan tryptophanyl-tRNA synthease grow on 4-fluorotyptophan and require tryptophan (total 20 μg/ml) Luria-Bertani (LB) L broth •when duplicate: resistant to indolmycin (1 to 5 μg/ml)(J. Bacteriol. 1983. 153: 1120-1123) Bacher, J. M. & A. D. Ellington
J. Bacteriol. 2001. 183: 5414-5425
trxA thioredoxin fipA tsnC thioredoxin killed by phage T7, but fail to produce bursts of the phage L broth supplemented with thymine (50 μg/ml) L broth Mark, D. F. et al.
Mol. Gen. Genet. 1977. 155: 145-152
trxB thioredoxin thioredoxin reductase unknown L-broth L broth Fuchs, J.
J. Bacteriol. 1977. 129: 967-972
tsaA tRNA-SAM methyltransferase ? tRNA (m6t6A37) methyltransferase unknown Luria-Bertani (LB) medium (Bertani, G. J. Bacteriol. 1951. 62: 293-300) or nutrient broth (0.8 %; Difco Laboratories) supplemented with 0.5 % NaCl L broth Qian, Q. et al.
J. Bacteriol. 1998. 180: 1808-1813
tsf Ts (Elongation) factor (EF-Ts) EF-Ts sensitive to nonpermissive temperarure (42 °C) polypeptone broth containing per liter 5 g of NaCl, 1 g of yeast extract, and 10 g of polypeptone L broth Kuwano, M. et al.
J. Mol. Biol. 1974. 86: 689-698
tsr taxis, serine and repellants cheD serine chemoreceptor, methyl accepting chemotaxis protein II, membrane receptor unresponsive to attractants (serine or alpha-aminoisobutyrate) or repellents (acetate, benzoate, indole, or leucine) tryptone broth L broth Springer, M. S. et al.
Proc. Natl. Acad. Sci. USA. 1977. 74: 3312-3316
tsx t-six T6rec, nupA nucleoside channel resistant to T6 TY medium containing (per liter) 10 g of bacto tryptone, 5 g of yeast extract, and 5 g of NaCl L broth •resistant to colicinK (Pugsley, A. P. J. Bacteriol. 1975. 123: 102-117) Hantke, K.
FEBS Lett. 1976. 70:109-112
tyrA tyrosine chrismate mutase T-prephenate dehydrogenase require tyrosine (500 microM-2 mM) L broth (Luria, S. E. et. al. J. Bacteriol. 1957. 74: 461-476) L broth Pittard, J. & B. J. Wallace
J. Bacteriol. 1966. 91: 1494-1508
tyrB tyrosine tyrosine aminotransferase require tyrosine (50 μg/ml) L broth containing 1% tryptone (Difco), 0.5 % yeast extract, 0.2 % glucose, and 0.5 % NaCl L broth •tyrB507 mutants require 50 μg/ml of tyrosine and phenylalanine even in L broth. (J. Bacteriol. 1977. 130: 429-440) Gelfand, D. H. & R. A. Steinberg
J. Bacteriol. 1977. 130: 429-440
tyrR tyrosine tyrR regulon repressor resistatnt to L-3-fluorotyrosine (0.1 mM) mineral medium 56 (Biochim. Biophys. Acta.1951.7: 585-599) supplemented with 0.5 % glucose L broth •sensitive to L-beta-thienylalamine (5 mM) •in aroG- aroH-: resistant to 4-aminophenylalanine (10-4 M)(J. Bacteriol. 1969. 97: 1234-1241) Camakaris, H. & J. Pittard
J. Bacteriol. 1982. 150: 70-75
tyrS tyrosine tyrosyl-tRNA synthase require tyrosine (1 mM) medium containing 5.32 g of KH2PO4, 10.6 g of K2HPO4, 2.0 g of (NH4)2SO4, 0.2 g of MgSO4, 2.0 g of glucose, and 0.005 g of thiamine per liter L broth •grow badly on broth
•otherwise sensitive to nonpermissive temperature (42oC) (Eur. J. Biochem. 1982. 125: 357-360) Schlesinger, A. & E. W. Nester
J. Bacteriol. 1969. 100: 167-175
tyrT tyrosine tyrTa suIII Su-3 Su-4 supC supF supO? tandemly duplicated tyrosine tRNA (tyrTV) unknown L broth containing 1 % Difco tryptone, 0.5 % Difco yeast extract, 0.5 % NaCl, and 0.1 % glucose L broth •in case of supC: suppress missense mutation (ochre)
•in case of supF: suppress missense mutation (amber) Kimura, M. et al.
J. Bacteriol. 1979. 140: 825-834
ubiA ubiquinone 4-hydroxybenzoate polyprenyltransferase unable to grow on malate (3 x 10-2 M) as sole carbon source tryptone-yeast extract broth L broth Cox, G. B. et al.
J. Bacteriol. 1968. 95: 1591-1598
ubiF ubiquinone reaction: 2-octaprenyl-3-methyl-6-methoxy-1, 4-benzoquinone to 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1, 4-benzoquinone unable to grow on malate (3 x 10-2 M) as sole carbon source (J. Bacteriol. 1968. 95: 1591-1598) tryptone-yeast extract broth (J. Bacteriol. 1968. 95: 1591-1598) L broth Young, I. G. et al.
J. Bacteriol. 1971. 105: 769-778
ugpA uptake glycerol phosphate psiB psiC glycerol P transport system, integral membrane protein grow on minimal glucose plates in the presence of 3,4-dihydroxybutyl-1-phosphonate (2.5 mM) LB or DYT medium (Miller, J. Experiments in molecular genetics. 1972. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.) L broth Schweizer, H. et al.
J. Bacteriol. 1982. 150: 1164-1171
uidA hexUronIDes gurA gusA beta-D-glucuronidase grow on methyl-beta-galacturonide (2 mg/ml) as the sole carbon source M63 mineral medium (Biochim. Biopys. Acta. 1958. 29: 579-587) supplemented or L-broth L broth •in glpT-: resistant to fosfomycin (J. Gen. Microbiol. 1977. 103: 37-43)
•in eda-: grow on glycerol + methylglucuronide (Mol. Gen. Genet. 1973. 120: 319-335) Novel, M. & G. Novel
J. Bacteriol. 1976. 127: 406-417
uidB hexUronIDes gusB glucuronide permease unknown (no mutant yet)
uidR hexUronIDes gusR regulatory gene form yellow colonies with a few drops of 10 mM p-nitrophenyl-beta-D-glucuronide and chloramphenicol (50 μg/ml) after overnight growth on glycerol-agar medium M63 mineral medium (Biochim. Biopys. Acta. 1958. 29: 579-587) supplemented or L-broth L broth Novel, M. & G. Novel
J. Bacteriol. 1976. 127: 406-417
umuC UcV mutator uvm UV induction of mutations, error-prone repair, forms complex with UmuD and UmuD' sensitive to UV Luria broth (L broth) L broth Kato, T. & Y. Shinoura
Mol. Gen. Genet. 1977. 156: 121-131.
ung uracil nucleic acid glycosylase urasil-DNA-glycosylase sensitive to urasil-containing T4 phage TY medium containing 1.0 % tryptone (Difco), 0.5 % yeast extract, and 1.0 % sodium chloride, pH 7.0 L broth Duncan, B. K. et al.
J. Bacteriol. 1978. 134: 1039-1045
upp uracil PRTase uraP uracil phosphoribosyltransferase resistant to azauracil (100 μg/ml) liquid broth containing 1 % Difco Bacto-tryptone, 0.5 % Difco yeast extract, 0.1 % glucose, and 0.5 % NaCl L broth Pierard, A. & N. Glansdorff
Mol. Gen. Genet. 1972. 118: 235-245
ushA UDP sugar hydrolase UDP-glucose hydrolase (5'-nucleotidase) resistant to 5-fluorouracil (2 μg/ml) plus AMP (1 mM) M9 medium (J. Mol. Biol. 1964. 9: 288-307) supplemented with glucose (0.2 %) L broth •unable to grow on AMP as carbon source Beacham, I. R. et al.
J. Bacteriol. 1973. 116: 957-964
uvrA UV resistant dar excision nuclease subunit A sensitive to UV M9 supplemented with 0.4 mg/ml of casamino acids L broth Boyce, R. P. & P. Haward-Flanders
Proc. Natl. Acad. Sci. USA. 1964. 51: 293-300
uvrB UV resistant dar-1 dar-6 excision nuclease subunit B sensitive to UV nutrient broth containing 8 g of Difco nutrient broth and 5 g of NaCl per lliter. L broth van de Putte, P. et al.
Mutat. Res. 1965. 2: 97-110
uvrC UV resistant excision nuclease subunit C sensitive to UV (20 J/m2) Luria broth (LB) L broth Forster, J. W. & P. Strike
Gene. 1985. 35: 71-82
uvrD UV resistant dar-2 dda mutU pdeB rad recL srjC uvr502 uvrE DNA-dependent ATPase I-DNA helicase II sensitive to UV lambda-broth containing 10 g of polypeptone (Daigo-Eiyo) and 2.5 % NaCl in one liter of water L broth •sensitive to hydrogen peroxide (1.0 mM) (J. Bacteriol. 1997. 179: 330-338) Ogawa, H. et al.
Mol. Gen. Genet. 1968. 101: 227-244
uxaA utilization hexuronate galacturonate altronate hydrolase unable to grow on galacturonate complete medium containing 10 μg/ml of Bactotryptone (Difco), 5 mg/ml of yeast extract (Difco), and 5 mg/ml of NaCl L broth Portalier, R. C. et al.
Mol. Gen. Genet. 1972. 118: 335-350
uxaC utilization hexuronate galacturonate uronate isomerase unable to grow on galacturonate or glucuronate (2.5 mg/ml) complete medium containing 10 mg/ml of Bactotryptone (Difco), 5 mg/ml of yeast extract (Difco), and 5 mg/ml of NaCl L broth Portalier, R. C. et al.
Mol. Gen. Genet. 1974. 128: 301-319
uxuA utilization hexuronate galacturonate mannonate hydrolase unable to grow on glucuronate (4 mg/ml) complete medium containing 10 mg/ml of Bactotryptone (Difco), 5 mg/ml of yeast extract (Difco), and 5 mg/ml of NaCl L broth Robert-Baudouy, J. et al.
Mol. Gen. Genet. 1972. 118: 351-362
uxuB utilization hexuronate galacturonate mannonate hydrolase unable to grow on glucuronate (2.5 mg/ml) complete medium containing 10 mg/ml of Bactotryptone (Difco), 5 mg/ml of yeast extract (Difco), and 5 mg/ml of NaCl L broth Robert-Baudouy, J. & R. C. Portalier
Mol. Gen. Genet. 1974. 131: 31-46
uxuR utilization hexuronate galacturonate regulateory gene for uxuBA operon grow on metylgalacturonide (2 mg/ml) as the sole carbon source L (Lennox, E. S. 1955. Virology. 1: 190-206) with 5 mg/ml of NaCl L broth Novel, M. & G. Novel
J. Bacteriol. 1976. 127: 418-432
valS valine val-act valyl-tRNA synthetase sensitive to nonpermissive temperature (40 °C) LB medium L broth Baker, M. et al.
J. Bacteriol. 1979. 139: 165-175
xthA exonuclease III exonuclease III unknown nutrient broth (Biochem. Biophys. Res. Commun. 1965. 18: 788) L broth Milcarek, C. & B. Weiss
J. Mol. Biol. 1972. 68: 303-318
xts unknown (no data)
xylA xylose D-xylose isomerase form colorless colonies on MacConkey xylose plates Luria Broth L broth Rosenfeld, S. A. et al.
Mol. Gen. Genet. 1984. 194: 410-415
xylB xylose xylulokinase form colorless colonies on MacConkey xylose plates and MacConkey xylulose plates Luria Broth L broth Rosenfeld, S. A. et al.
Mol. Gen. Genet. 1984. 194: 410-415