MG1655
W3110
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Gene Report : ispH
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Gen Microbiol. 1984;130(6):1315-23.
Temperature-sensitive beta-lactam-tolerant mutants of Escherichia coli.
Shimmin LC, Vanderwel D, Harkness RE, Currie BR, Galloway CA, Ishiguro EE. ( 6384423 )
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  ispH
Related strains
     Strains Search Search strains by gene name:  ispH   Search strains by all related name:  ispH b0029 ECK0030 JW0027 lytB yaaE

General information  (Go to Linear View:)
 Gene Name ispH  
 Alternative name b0029,ECK0030,JW0027,lytB,yaaE  
 Location, Length 26,277 - 27,227 (  +  ) ;   0.57 min ; 951 (bp) ,   316 (aa) Go to Linear View
 Product 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, 4Fe-4S protein  
 Note ECK0030:JW0027:b0029  
 Function  
 PID 21321911  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) ISPH_ECOLI ( P62623 ; P22565 )
    -  Protein name RecName: Full=4-hydroxy-3-methylbut-2-enyl diphosphate reductase; EC=1.17.1.2;  
    -  Synonyms  
    -  Gene name Name=ispH; Synonyms=lytB, yaaE; OrderedLocusNames=b0029, JW0027;  

 Linear View
View Location
   15.0  –  40.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP  
    PDB
        PSI-BLAST  
    SWISS-PROT
         BLAST RecName: Full=4-hydroxy-3-methylbut-2-enyl diphosphate reductase  
         PSI-BLAST RecName: Full=4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    nr
         BLAST IspH protein [Escherichia coli CFT073]  
Pfam 27.0   (database updated : 2013-03 )
    Pfam

Other Cross-Reference
    EcoCyc ispH 

Sequences
Amino acid
FASTA format
0001 MQILLANPRG FCAGVDRAIS IVENALAIYG APIYVRHEVV HNRYVVDSLR ERGAIFIEQI SEVPDGAILI 
0071 FSAHGVSQAV RNEAKSRDLT VFDATCPLVT KVHMEVARAS RRGEESILIG HAGHPEVEGT MGQYSNPEGG 
0141 MYLVESPDDV WKLTVKNEEK LSFMTQTTLS VDDTSDVIDA LRKRFPKIVG PRKDDICYAT TNRQEAVRAL 
0211 AEQAEVVLVV GSKNSSNSNR LAELAQRMGK RAFLIDDAKD IQEEWVKEVK CVGVTAGASA PDILVQNVVA 
0281 RLQQLGGGEA IPLEGREENI VFEVPKELRV DIREVD

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             GGCGACTCCA TTACCGTTGA TTTCAACCAT 
-070 CCGCTGGCCG GGCAGACCGT TCATTTTGAT ATTGAAGTGC TGGAAATCGA TCCGGCACTG GAGGCGTAAC 
0001 atgcagatcc tgttggccaa cccgcgtggt ttttgtgccg gggtagaccg cgctatcagc attgttgaaa 
0071 acgcgctggc catttacggc gcaccgatat atgtccgtca cgaagtggta cataaccgct atgtggtcga 
0141 tagcttgcgt gagcgtgggg ctatctttat tgagcagatt agcgaagtac cggacggcgc gatcctgatt 
0211 ttctccgcac acggtgtttc tcaggcggta cgtaacgaag caaaaagtcg cgatttgacg gtgtttgatg 
0281 ccacctgtcc gctggtgacc aaagtgcata tggaagtcgc ccgcgccagt cgccgtggcg aagaatctat 
0351 tctcatcggt cacgccgggc acccggaagt ggaagggaca atgggccagt acagtaaccc ggaaggggga 
0421 atgtatctgg tcgaatcgcc ggacgatgtg tggaaactga cggtcaaaaa cgaagagaag ctctccttta 
0491 tgacccagac cacgctgtcg gtggatgaca cgtctgatgt gatcgacgcg ctgcgtaaac gcttcccgaa 
0561 aattgtcggt ccgcgcaaag atgacatctg ctacgccacg actaaccgtc aggaagcggt acgcgccctg 
0631 gcagaacagg cggaagttgt gttggtggtc ggttcgaaaa actcctccaa ctccaaccgt ctggcggagc 
0701 tggcccagcg tatgggcaaa cgcgcgtttt tgattgacga tgcgaaagac atccaggaag agtgggtgaa 
0771 agaggttaaa tgcgtcggcg tgactgcggg cgcatcggct ccggatattc tggtgcagaa tgtggtggca 
0841 cgtttgcagc agctgggcgg tggtgaagcc attccgctgg aaggccgtga agaaaacatt gttttcgaag 
0911 tgccgaaaga gctgcgtgtc gatattcgtg aagtcgatta aGTCATTAGC AGCCTAAGTT ATGCGAAAAT 
0981 GCCGGTCTTG TTACCGGCAT TTTTTATGGA GAAAACATGC GTTTACCTAT CTTCCTCGAT ACTGACCCCG 
1051 G