MG1655
W3110
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Gene Report : dnaE
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)  
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  dnaE
Related strains
     Strains Search Search strains by gene name:  dnaE   Search strains by all related name:  dnaE b0184 ECK0183 JW0179 polC sdgC

General information  (Go to Linear View:)
 Gene Name dnaE  
 Alternative name b0184,ECK0183,JW0179,polC,sdgC  
 Location, Length 205,126 - 208,608 (  +  ) ;   4.41 min ; 3483 (bp) ,   1160 (aa) Go to Linear View
 Product DNA polymerase III alpha subunit  
 Note ECK0183:JW0179:b0184  
 Function  
 PID 4902925  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) DPO3A_ECOLI ( P10443 )
    -  Protein name RecName: Full=DNA polymerase III subunit alpha; EC=2.7.7.7;  
    -  Synonyms  
    -  Gene name Name=dnaE; Synonyms=polC; OrderedLocusNames=b0184, JW0179;  

 Linear View
View Location
   195.0  –  220.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP  
    PDB
        PSI-BLAST  
    SWISS-PROT
        BLAST  
        PSI-BLAST
    nr
         BLAST DNA polymerase III alpha subunit [Escherichia coli str. K-12 substr. MG1655]  
Pfam 27.0   (database updated : 2013-03 )
    Pfam

Other Cross-Reference
    EcoCyc dnaE 

MMBR References
J Bacteriol. 1992;174:4166-4168
Overproduction of DnaE protein ( subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I.
Witkin, E. M., and V. Roegner-Maniscalco.

Sequences
Amino acid
FASTA format
0001 MSEPRFVHLR VHSDYSMIDG LAKTAPLVKK AAALGMPALA ITDFTNLCGL VKFYGAGHGA GIKPIVGADF 
0071 NVQCDLLGDE LTHLTVLAAN NTGYQNLTLL ISKAYQRGYG AAGPIIDRDW LIELNEGLIL LSGGRMGDVG 
0141 RSLLRGNSAL VDECVAFYEE HFPDRYFLEL IRTGRPDEES YLHAAVELAE ARGLPVVATN DVRFIDSSDF 
0211 DAHEIRVAIH DGFTLDDPKR PRNYSPQQYM RSEEEMCELF ADIPEALANT VEIAKRCNVT VRLGEYFLPQ 
0281 FPTGDMSTED YLVKRAKEGL EERLAFLFPD EEERLKRRPE YDERLETELQ VINQMGFPGY FLIVMEFIQW 
0351 SKDNGVPVGP GRGSGAGSLV AYALKITDLD PLEFDLLFER FLNPERVSMP DFDVDFCMEK RDQVIEHVAD 
0421 MYGRDAVSQI ITFGTMAAKA VIRDVGRVLG HPYGFVDRIS KLIPPDPGMT LAKAFEAEPQ LPEIYEADEE 
0491 VKALIDMARK LEGVTRNAGK HAGGVVIAPT KITDFAPLYC DEEGKHPVTQ FDKSDVEYAG LVKFDFLGLR 
0561 TLTIINWALE MINKRRAKNG EPPLDIAAIP LDDKKSFDML QRSETTAVFQ LESRGMKDLI KRLQPDCFED 
0631 MIALVALFRP GPLQSGMVDN FIDRKHGREE ISYPDVQWQH ESLKPVLEPT YGIILYQEQV MQIAQVLSGY 
0701 TLGGADMLRR AMGKKKPEEM AKQRSVFAEG AEKNGINAEL AMKIFDLVEK FAGYGFNKSH SAAYALVSYQ 
0771 TLWLKAHYPA EFMAAVMTAD MDNTEKVVGL VDECWRMGLK ILPPDINSGL YHFHVNDDGE IVYGIGAIKG 
0841 VGEGPIEAII EARNKGGYFR ELFDLCARTD TKKLNRRVLE KLIMSGAFDR LGPHRAALMN SLGDALKAAD 
0911 QHAKAEAIGQ ADMFGVLAEE PEQIEQSYAS CQPWPEQVVL DGERETLGLY LTGHPINQYL KEIERYVGGV 
0981 RLKDMHPTER GKVITAAGLV VAARVMVTKR GNRIGICTLD DRSGRLEVML FTDALDKYQQ LLEKDRILIV 
1051 SGQVSFDDFS GGLKMTAREV MDIDEAREKY ARGLAISLTD RQIDDQLLNR LRQSLEPHRS GTIPVHLYYQ 
1121 RADARARLRF GATWRVSPSD RLLNDLRGLI GSEQVELEFD 

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CGCGACCGAA CACCATCGGC GCAGCTTTGG 
-070 GCCTGTCAAA CGCGCACTGG GACTTGCGTC CTGATTCTTG TGTCGAGATT AAGTAAACCG GAATCTGAAG 
0001 atgtctgaac cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ctggccaaaa 
0071 ccgcaccgtt ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt tcaccaacct 
0141 ttgtggtctg gtgaagttct acggagcggg acatggcgca gggattaagc ctatcgtcgg ggcagatttt 
0211 aacgtccagt gcgacctgct gggtgatgag ttaacccacc tgacggtact ggcggcgaac aataccggct 
0281 atcagaatct gacgttgctg atctcaaaag cgtatcagcg cgggtacggt gccgccgggc cgatcatcga 
0351 tcgcgactgg cttatcgaat taaacgaagg gttgatcctt ctttccggcg gacgcatggg cgacgtcgga 
0421 cgcagtcttt tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg 
0491 atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc tatctgcacg cggcggtgga 
0561 actggcggaa gcgcgcggtt tgcccgtcgt ggcgaccaac gacgtgcgct ttatcgacag cagcgacttt 
0631 gacgcacacg aaatccgcgt cgcgatccac gacggcttta ccctcgacga tcctaaacgc ccgcgtaact 
0701 attcgccgca gcaatatatg cgtagcgaag aggagatgtg tgagctgttt gccgacatcc ccgaagccct 
0771 tgccaacacc gttgagatcg ccaaacgctg taacgtaacc gtgcgtcttg gtgaatactt cctgccgcag 
0841 ttcccgaccg gggacatgag caccgaagat tatctggtca agcgtgcaaa agagggcctg gaagagcgtc 
0911 tggccttttt attccctgat gaggaagaac gtcttaagcg ccgcccggaa tatgacgaac gtctggagac 
0981 tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg ttatggaatt tatccagtgg 
1051 tcgaaagata acggcgtacc ggtagggcca ggccgtggct ccggtgcggg ttcactggtg gcctacgcgc 
1121 tgaaaatcac cgacctcgat ccgctggaat ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt 
1191 ctccatgcct gacttcgacg ttgacttctg tatggagaaa cgcgatcagg ttatcgagca cgtagcggac 
1261 atgtacggtc gtgatgcggt atcgcagatc atcaccttcg gtacaatggc ggcgaaagcg gtgatccgcg 
1331 acgtaggccg cgtgctgggg catccgtacg gctttgtcga tcgtatctcg aaactgatcc cgcccgatcc 
1401 ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa tctacgaagc ggatgaagaa 
1471 gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg tcacccgtaa cgccggtaag cacgccggtg 
1541 gggtggttat cgcgccgacc aaaattaccg attttgcgcc gctttactgc gatgaagagg gcaaacatcc 
1611 ggtcacccag tttgataaaa gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt 
1681 acgctcacca tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccgccgc 
1751 tggatatcgc tgcgatcccg ctggatgata agaaaagctt cgacatgctg caacgctcgg aaaccacggc 
1821 ggtattccag cttgaatcgc gcggcatgaa ggacctgatc aagcgtctac aacctgactg cttcgaagat 
1891 atgatcgccc tagtggcact gttccgcccc ggtccgttgc aatcagggat ggtggataac tttatcgacc 
1961 gtaaacatgg tcgtgaagag atctcctatc cggacgtaca gtggcagcat gaaagcctga aaccggtact 
2031 ggagccaacc tacggcatta tcctgtatca ggaacaggtc atgcagattg cgcaggtgct ttctggttat 
2101 accctcggtg gcgcggatat gctgcgtcgt gcgatgggta agaaaaagcc ggaagagatg gctaagcaac 
2171 gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa cgctgaactg gcgatgaaaa tcttcgacct 
2241 ggtggagaaa ttcgctggtt acggatttaa caaatcgcac tctgcggcct atgctttggt gtcatatcaa 
2311 acgttatggc tgaaagcgca ctatcctgcg gagtttatgg cggcggtaat gaccgccgat atggacaaca 
2381 ccgagaaggt ggtgggtctg gtggatgagt gctggcggat ggggctgaaa atcctgccac cagatataaa 
2451 ctccggtctt taccatttcc acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc gatcaaaggg 
2521 gtcggtgaag gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg 
2591 atctctgcgc ccgtaccgac accaaaaagt tgaaccgtcg cgtgctggaa aaactgatca tgtccggggc 
2661 gtttgaccgt cttgggccac atcgcgcagc gctgatgaac tcgctgggcg atgcgttaaa agcggcagat 
2731 caacacgcga aagcggaagc tatcggtcag gccgatatgt tcggcgtgct ggccgaagag ccggaacaaa 
2801 ttgaacaatc ctacgccagc tgccaaccgt ggccggagca ggtggtatta gatggggaac gtgaaacgtt 
2871 aggcctgtac ctgaccggac accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta 
2941 aggctgaaag acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc 
3011 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg ggcggctgga 
3081 agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa aagaccgcat acttatcgtc 
3151 agcggacagg tcagctttga tgacttcagc ggtgggctta aaatgaccgc tcgcgaagtg atggatattg 
3221 acgaagcccg ggaaaaatat gctcgcgggc ttgctatctc gctgacggac aggcaaattg atgaccagct 
3291 tttaaaccga ctccgtcagt ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag 
3361 agggcggatg cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa 
3431 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taaTACAGGA ATACTATGAG 
3501 TCTGAATTTC CTTGATTTTG AACAGCCGAT TGCAGAGCTG GAAGCGAAAA TCGATTCTCT GACTGCGGTT 
3571 AGCCGTCAGG ATG