MG1655
W3110
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Gene Report : serC
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
J Bacteriol. 1990;172(11):6518-28.
Metabolic relationships between pyridoxine (vitamin B6) and serine biosynthesis in Escherichia coli K-12.
Lam HM, Winkler ME. ( 2121717 )
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  serC
Related strains
     Strains Search Search strains by gene name:  serC   Search strains by all related name:  serC b0907 ECK0898 JW0890 pdxC pdxF

General information  (Go to Linear View:)
 Gene Name serC  
 Alternative name b0907,ECK0898,JW0890,pdxC,pdxF  
 Location, Length 958,075 - 959,163 (  +  ) ;   20.62 min ; 1089 (bp) ,   362 (aa) Go to Linear View
 Product 3-phosphoserine/phosphohydroxythreonine aminotransferase  
 Note ECK0898:JW0890:b0907  
 Function  
 PID 1651429  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) SERC_ECOLI ( P23721 ; P78266 )
    -  Protein name RecName: Full=Phosphoserine aminotransferase; EC=2.6.1.52; AltName: Full=Phosphohydroxythreonine aminotransferase; Short=PSAT;  
    -  Synonyms  
    -  Gene name Name=serC; Synonyms=pdxC, pdxF; OrderedLocusNames=b0907, JW0890;  
    -  Comments
  • FUNCTION:Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. Is involved in both pyridoxine and serine biosynthesis.
  • CATALYTIC ACTIVITY:O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate.
  • CATALYTIC ACTIVITY:4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate.
  • COFACTOR:Binds 1 pyridoxal phosphate per subunit.
  • BIOPHYSICOCHEMICAL PROPERTIES:Kinetic parameters:KM=15 uM for 3-phosphonooxypyruvate; KM=17 uM for O(3)-phospho-L-serine; KM=110 uM for 4-phosphonooxy-L-threonine; Vmax=45 nmol/sec/mg enzyme with 3-phosphonooxypyruvate as substrate; Vmax=9.9 nmol/sec/mg enzyme with O(3)-phospho-L-serine as substrate; Vmax=3.8 nmol/sec/mg enzyme with 4-phosphonooxy-L-threonine as substrate; Note=No activity could be observed with non-phosphorylated substrates; pH dependence:Optimum pH is 7.5-8.8;
  • PATHWAY:Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate:step 2/3.
  • PATHWAY:Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate:step 3/5.
  • SUBUNIT:Homodimer.
  • INTERACTION:P00963:asnA; NbExp=1; IntAct=EBI-557952, EBI-1117481; P0A6Y8:dnaK; NbExp=1; IntAct=EBI-557952, EBI-542092; P14081:selB; NbExp=1; IntAct=EBI-557952, EBI-551705;
  • SUBCELLULAR LOCATION:Cytoplasm.
  • SIMILARITY:Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.  

    •  Linear View
    View Location
       945.0  –  970.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP  
        PDB
             PSI-BLAST  
        SWISS-PROT
             BLAST RecName: Full=Phosphoserine aminotransferase; AltName: Full=Phosphohydroxythreonine aminotransferase; Short=PSAT  
            PSI-BLAST
        nr
             BLAST 3-phosphoserine/phosphohydroxythreonine aminotransferase [Escherichia coli str. K-12 substr. MG1655]  
    Pfam 27.0   (database updated : 2013-03 )
        Pfam

    Other Cross-Reference
        EcoCyc serC 

    Sequences
    Amino acid
    FASTA format 
    0001 MAQIFNFSSG PAMLPAEVLK QAQQELRDWN GLGTSVMEVS HRGKEFIQVA EEAEKDFRDL LNVPSNYKVL 
0071 FCHGGGRGQF AAVPLNILGD KTTADYVDAG YWAASAIKEA KKYCTPNVFD AKVTVDGLRA VKPMREWQLS 
0141 DNAAYMHYCP NETIDGIAID ETPDFGADVV VAADFSSTIL SRPIDVSRYG VIYAGAQKNI GPAGLTIVIV 
0211 REDLLGKANI ACPSILDYSI LNDNGSMFNT PPTFAWYLSG LVFKWLKANG GVAEMDKINQ QKAELLYGVI 
0281 DNSDFYRNDV AKANRSRMNV PFQLADSALD KLFLEESFAA GLHALKGHRV VGGMRASIYN AMPLEGVKAL 
0351 TDFMVEFERR HG
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             GTCACTGAAT GATAAAACCG ATAGCCACAG 
-070 GAATAATGTA TTACCTGTGG TCGCAATCGA TTGACCGCGG GTTAATAGCA ACGCAACGTG GTGAGGGGAA 
0001 atggctcaaa tcttcaattt tagttctggt ccggcaatgc taccggcaga ggtgcttaaa caggctcaac 
0071 aggaactgcg cgactggaac ggtcttggta cgtcggtgat ggaagtgagt caccgtggca aagagttcat 
0141 tcaggttgca gaggaagccg agaaggattt tcgcgatctt cttaatgtcc cctccaacta caaggtatta 
0211 ttctgccatg gcggtggtcg cggtcagttt gctgcggtac cgctgaatat tctcggtgat aaaaccaccg 
0281 cagattatgt tgatgccggt tactgggcgg caagtgccat taaagaagcg aaaaaatact gcacgcctaa 
0351 tgtctttgac gccaaagtga ctgttgatgg tctgcgcgcg gttaagccaa tgcgtgaatg gcaactctct 
0421 gataatgctg cttatatgca ttattgcccg aatgaaacca tcgatggtat cgccatcgac gaaacgccag 
0491 acttcggcgc agatgtggtg gtcgccgctg acttctcttc aaccattctt tcccgtccga ttgacgtcag 
0561 ccgttatggt gtaatttacg ctggcgcgca gaaaaatatc ggcccggctg gcctgacaat cgtcatcgtt 
0631 cgtgaagatt tgctgggcaa agcgaatatc gcgtgtccgt cgattctgga ttattccatc ctcaacgata 
0701 acggctccat gtttaacacg ccgccgacat ttgcctggta tctatctggt ctggtcttta aatggctgaa 
0771 agcgaacggc ggtgtagctg aaatggataa aatcaatcag caaaaagcag aactgctata tggggtgatt 
0841 gataacagcg atttctaccg caatgacgtg gcgaaagcta accgttcgcg gatgaacgtg ccgttccagt 
0911 tggcggacag tgcgcttgac aaattgttcc ttgaagagtc ttttgctgct ggccttcatg cactgaaagg 
0981 tcaccgtgtg gtcggcggaa tgcgcgcttc tatttataac gccatgccgc tggaaggcgt taaagcgctg 
1051 acagacttca tggttgagtt cgaacgccgt cacggttaaT GCCGAAATTT TGCTTAATCC CCACAGCCAG 
1121 CCTGTGGGGT TTTTATTTCT GTTGTAGAGA GTTGAGTTCA TGGAATCCCT GACGTTACAA CCCATCGCT