MG1655
W3110
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Gene Report : hcp
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Eur J Biochem. 2000;267(3):666-76.
The hybrid-cluster protein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and [2Fe-2S].
van den Berg WA, Hagen WR, van Dongen WM. ( 10651802 )
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  hcp
Related strains
     Strains Search Search strains by gene name:  hcp   Search strains by all related name:  hcp b0873 ECK0864 JW0857 ybjW

General information  (Go to Linear View:)
 Gene Name hcp  
 Alternative name b0873,ECK0864,JW0857,ybjW  
 Location, Length 912,584 - 914,236 (  -  ) ;   19.64 min ; 1653 (bp) ,   550 (aa) Go to Linear View
 Product hybrid-cluster [4Fe-2S-2O] protein in anaerobic terminal reductases  
 Note ECK0864:JW0857:b0873  
 Function  
 PID 85674784  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) HCP_ECOLI ( P75825 ; Q9R7R0; Q9R7R1 )
    -  Protein name RecName: Full=Hydroxylamine reductase; EC=1.7.-.-; AltName: Full=Hybrid-cluster protein; Short=HCP;  
    -  Synonyms  
    -  Gene name Name=hcp; Synonyms=ybjW; OrderedLocusNames=b0873, JW0857;  
    -  Comments
  • FUNCTION:Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O. Is also able to reduce hydroxylamine analogs such as methylhydroxylamine and hydroxyquinone. Might have a role as a scavenger of potentially toxic by-products of nitrate metabolism.
  • COFACTOR:Binds 1 2Fe-2S cluster.
  • COFACTOR:Binds 1 hybrid 4Fe-2O-2S cluster.
  • ENZYME REGULATION:Inhibited by oxygen. Activated by cyanide except in the prolonged presence of excess cyanide, where the enzyme is inactivated.
  • BIOPHYSICOCHEMICAL PROPERTIES:pH dependence:Optimum pH is 9.0;
  • INTERACTION:P07003:poxB; NbExp=1; IntAct=EBI-561591, EBI-909338;
  • SUBCELLULAR LOCATION:Cytoplasm.
  • INDUCTION:Is expressed under anaerobic conditions in the presence of either nitrate or nitrite.
  • SIMILARITY:Belongs to the HCP family.  

    •  Linear View
    View Location
       900.0  –  925.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP  
        PDB
             PSI-BLAST  
        SWISS-PROT
            BLAST  
             PSI-BLAST RecName: Full=Hydroxylamine reductase; AltName: Full=Hybrid-cluster protein; Short=HCP
        nr
             BLAST hybrid-cluster [4Fe-2S-2O] protein in anaerobic terminal reductases [Escherichia coli str. K-12 substr. W3110]  
    Pfam 27.0   (database updated : 2013-03 )
        Pfam

    Other Cross-Reference
        EcoCyc hcp 

    Sequences
    Amino acid
    FASTA format 
    0001 MFCVQCEQTI RTPAGNGCSY AQGMCGKTAE TSDLQDLLIA ALQGLSAWAV KAREYGIINH DVDSFAPRAF 
0071 FSTLTNVNFD SPRIVGYARE AIALREALKA QCLAVDANAR VDNPMADLQL VSDDLGELQR QAAEFTPNKD 
0141 KAAIGENILG LRLLCLYGLK GAAAYMEHAH VLGQYDNDIY AQYHKIMAWL GTWPADMNAL LECSMEIGQM 
0211 NFKVMSILDA GETGKYGHPT PTQVNVKATA GKCILISGHD LKDLYNLLEQ TEGTGVNVYT HGEMLPAHGY 
0281 PELRKFKHLV GNYGSGWQNQ QVEFARFPGP IVMTSNCIID PTVGAYDDRI WTRSIVGWPG VRHLDGDDFS 
0351 AVITQAQQMA GFPYSEIPHL ITVGFGRQTL LGAADTLIDL VSREKLRHIF LLGGCDGARG ERHYFTDFAT 
0421 SVPDDCLILT LACGKYRFNK LEFGDIEGLP RLVDAGQCND AYSAIILAVT LAEKLGCGVN DLPLSLVLSW 
0491 FEQKAIVILL TLLSLGVKNI VTGPTAPGFL TPDLLAVLNE KFGLRSITTV EEDMKQLLSA
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             tcaatctccc ttaaagttgc atgaaaaatc 
-070 ccttttatcc ccgcgttaag cgtcttaacc ttaaacatgt atattaaata taactttaaa aggtgtgatc 
0001 atgttttgtg tgcaatgtga acaaactatc cgtactccgg caggaaacgg ctgctcatac gcgcagggga 
0071 tgtgtggtaa aacggcggaa acttctgacc ttcaggattt actcatcgcg gcgctgcaag ggctttctgc 
0141 ctgggcggta aaagcgcgtg aatacggcat catcaaccac gatgttgaca gcttcgcgcc acgtgccttt 
0211 ttctcaaccc tgaccaacgt taacttcgat tctccgcgta ttgtcggcta cgctcgtgaa gcgattgccc 
0281 tgcgcgaggc gctgaaagcg caatgcctgg ctgtagatgc caacgcgcgc gtcgataacc cgatggctga 
0351 cctgcaactg gtgagcgacg atctcggcga gctgcaacgt caggcagcag aatttacccc taacaaagat 
0421 aaagcggcga ttggcgaaaa cattctcggc ctgcgtctgc tgtgcctgta tggcctgaaa ggtgcggcgg 
0491 cctatatgga acacgcgcat gttctcggtc aatacgacaa cgatatttat gcccagtacc ataaaatcat 
0561 ggcgtggctg gggacctggc ctgccgatat gaacgcgctt cttgagtgtt caatggaaat cggccagatg 
0631 aacttcaaag tgatgagcat tctggatgca ggcgaaaccg gtaaatacgg tcacccgacg ccaacccagg 
0701 tcaacgtcaa agcgacggcg ggtaaatgca ttctgatttc cggtcacgat ctcaaagatc tctacaacct 
0771 gctggaacag accgaaggca cgggcgttaa tgtctacacc cacggcgaaa tgctgcctgc gcatggctac 
0841 ccggagctgc gtaaattcaa gcatctggtc ggtaactacg gcagcggctg gcagaatcag caagtggagt 
0911 tcgctcgttt ccctggcccc atcgtgatga cctcgaactg catcatcgac ccaaccgtag gcgcttatga 
0981 cgatcgtatc tggacccgca gcattgttgg ctggcctggc gtgcgtcatc tggacggtga tgatttctct 
1051 gcggttatca cccaggcgca acagatggca ggcttcccgt acagcgaaat tccgcacctt atcaccgtgg 
1121 gttttggtcg ccagacgctg cttggcgctg ctgatacgct gattgatctg gtgagccgtg aaaaactgcg 
1191 tcatatcttc ctgcttggtg gctgtgacgg cgcacgcggc gagcgccact acttcaccga tttcgccacc 
1261 agcgtgccgg atgactgcct gatcctgacc ctcgcctgtg gtaaatatcg ctttaacaaa cttgagtttg 
1331 gcgatatcga aggtctgccg cgtctggtag atgcaggtca atgtaacgat gcttactcag cgattattct 
1401 ggctgtcact ctggcagaga aactgggctg cggtgtcaac gatctgccgc tgtcgctggt gctctcctgg 
1471 tttgaacaga aagcgatcgt cattctgctg acgctgcttt ctctgggcgt gaaaaatatc gtcaccggcc 
1541 cgactgcacc tggtttcctg acaccagacc tgctggcggt gctgaacgag aaattcggcc tgcgttctat 
1611 caccactgtt gaagaagaca tgaagcaact gttgagcgcg taaggagttt gtcgatgacg atgccaacga 
1681 atcaatgccc gtggcggatg caggttcatc acattacgca agaaacgccg gatgtgtgga cgatttccct 
1751 gat