|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
|
|
Related gene (MG1655 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
leuB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
leuB
Search strains by all related name:
leuB b0073 ECK0075 JW5807
|
General information (Go to Linear View: )
|
|
Gene Name
|
leuB
|
|
Alternative name
|
b0073,ECK0075,JW5807
|
|
Location, Length
|
80,867 - 81,958
(
-
)
;
1.74
min
;
1092
(bp) ,
363
(aa)
|
|
Product
|
3-isopropylmalate dehydrogenase
|
|
Note
|
ECK0075:JW5807:b0073
|
|
Function
|
|
|
PID
|
21321955
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
LEU3_ECOLI
(
P30125
;
P78043
)
|
|
- Protein name
|
RecName: Full=3-isopropylmalate dehydrogenase; EC=1.1.1.85; AltName: Full=Beta-IPM dehydrogenase; Short=IMDH; AltName: Full=3-IPM-DH;
|
|
- Synonyms
|
|
|
- Gene name
|
Name=leuB; OrderedLocusNames=b0073, JW5807;
|
|
- Comments
|
FUNCTION:Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
CATALYTIC ACTIVITY:(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
COFACTOR:Binds 1 magnesium or manganese ion per subunit.
PATHWAY:Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate:step 3/4.
SUBUNIT:Homodimer.
SUBCELLULAR LOCATION:Cytoplasm.
SIMILARITY:Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.
|
|
MMBR References
|
J Bacteriol. 1977;129(2):1078-90.
Isolation and characterization of lambda pleu bacteriophages.
Davis MG, Calvo JM.
(
320178
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSKNYHIAVL PGDGIGPEVM TQALKVLDAV RNRFAMRITT SHYDVGGAAI DNHGQPLPPA TVEGCEQADA
0071 VLFGSVGGPK WEHLPPDQQP ERGALLPLRK HFKLFSNLRP AKLYQGLEAF CPLRADIAAN GFDILCVREL
0141 TGGIYFGQPK GREGSGQYEK AFDTEVYHRF EIERIARIAF ESARKRRHKV TSIDKANVLQ SSILWREIVN
0211 EIATEYPDVE LAHMYIDNAT MQLIKDPSQF DVLLCSNLFG DILSDECAMI TGSMGMLPSA SLNEQGFGLY
0281 EPAGGSAPDI AGKNIANPIA QILSLALLLR YSLDADDAAC AIERAINRAL EEGIRTGDLA RGAAAVSTDE
0351 MGDIIARYVA EGV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ccatggtgca cgttctgaac aatatctggc
-070 gtgccgcaga agtcgaaaaa gagttgcaac gcaaagctca acacaacgaa aacaacaagg aaaccgtgtg
0001 atgtcgaaga attaccatat tgccgtattg ccgggggacg gtattggtcc ggaagtgatg acccaggcgc
0071 tgaaagtgct ggatgccgtg cgcaaccgct ttgcgatgcg catcaccacc agccattacg atgtaggcgg
0141 cgcagccatt gataaccacg ggcaaccact gccgcctgcg acggttgaag gttgtgagca agccgatgcc
0211 gtgctgtttg gctcggtagg cggcccgaag tgggaacatt taccaccaga ccagcaacca gaacgcggcg
0281 cgctgctgcc tctgcgtaag cacttcaaat tattcagcaa cctgcgcccg gcaaaactgt atcaggggct
0351 ggaagcattc tgtccgctgc gtgcagacat tgccgcaaac ggcttcgaca tcctgtgtgt gcgcgaactg
0421 accggcggca tctatttcgg tcagccaaaa ggccgcgaag gtagcggaca atatgaaaaa gcctttgata
0491 ccgaggtgta tcaccgtttt gagatcgaac gtatcgcccg catcgcgttt gaatctgctc gcaagcgtcg
0561 ccacaaagtg acgtcgatcg ataaagccaa cgtgctgcaa tcctctattt tatggcggga gatcgttaac
0631 gagatcgcca cggaataccc ggatgtcgaa ctggcgcata tgtacatcga caacgccacc atgcagctga
0701 ttaaagatcc atcacagttt gacgttctgc tgtgctccaa cctgtttggc gacattctgt ctgacgagtg
0771 cgcaatgatc actggctcga tggggatgtt gccttccgcc agcctgaacg agcaaggttt tggactgtat
0841 gaaccggcgg gcggctcggc accagatatc gcaggcaaaa acatcgccaa cccgattgca caaatccttt
0911 cgctggcact gctgctgcgt tacagcctgg atgccgatga tgcggcttgc gccattgaac gcgccattaa
0981 ccgcgcatta gaagaaggca ttcgcaccgg ggatttagcc cgtggcgctg ccgccgttag taccgatgaa
1051 atgggcgata tcattgcccg ctatgtagca gaaggggtgt aatcatggct aagacgttat acgaaaaatt
1121 gttcgacgct cacgttgtgt acgaagccga aaacgaaacc ccactgttat atatcgaccg ccacctggtg
1191 ca
|
